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Araldite

Araldite is a type of epoxy resin adhesive that is widely used in various industries, including aerospace, automotive, and construction.
It is known for its high strength, durability, and resistance to chemicals and heat.
Araldite is commonly used for bonding, sealing, and repairing a variety of materials, such as metal, glass, ceramic, and plastics.
The product is available in different formulations to meet specific application requirements, and its performance can be optimized through the use of AI-driven comparisons across literature, preprints, and patents.
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Most cited protocols related to «Araldite»

Muscle tissue was prepared as described in Schwarz et al. (2000) (link). For the preparation of rodent brain tissue the animals were perfused transcardially first with 30 ml of phosphate-buffered saline and then with 40 ml of fixative solution (4% paraformaldehyde in 0.1M PBS [pH 7.4]). The brain tissue was then removed and kept in fixative over night at 4 °C. After being washed twice in PBS, tissue slices (0.2 to 1.5 mm thick) were cut on a vibratome (752 M Vibroslice, Campden Instruments, Leichester, United Kingdom) and kept for 24 h in PBS at 4 °C. Pieces about 1.5 mm in size were then excised and washed three times for 30 min each in cacodylate buffer at pH 7.4.The tissue was postfixed for 2 h in 2% osmium tetroxide/1.5% potassium ferric cyanide in aqueous solution at room temperature. Then the tissue was subjected to a contrast enhancement step by soaking it over night in a solution of 4% uranyl acetate in a 25% methanol/75% water mixture (Stempak and Ward 1964 (link)) at room temperature. After that the tissue was dehydrated in a methanol sequence (25%, 70%, 90%, and 100% for 30 min each) followed by infiltration of the epoxy (Spurr, Epon 812, or Araldite, all from Serva, Heidelberg, Germany) monomer (epoxy/methanol 1:1, for 3 h rotation at room temperature; epoxy/methanol 3:1, overnight at 4°C; pure epoxy, 3 h rotating at room temperature). Polymerization was 48 h at 60 °C for Epon and at 70 °C for Spurr and Araldite. The block face was trimmed to a width of several hundred microns and a length of about 500 μm using either a conventional microtome or a sharp knife. SEM images of the untrimmed block face can be used to select the desired field of view before the final trimming step producing the desired small cutting pyramid.
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Publication 2004
Animals araldite Brain Buffers Cacodylate EPON Epon 812 Epoxy Resins Face Fixatives Methanol Microtomy Muscle Tissue Osmium Tetroxide paraform Phosphates Polymerization Potassium Cyanide Rodent Saline Solution spurr resin Tissues uranyl acetate
At present, it is possible to study the ultrastructure of large numbers of synapses within 3D samples of brain tissue. Indeed, using combined focused ion beam milling and scanning electron microscopy (FIB/SEM), it has been shown that virtually all synaptic junctions can be identified regardless of the plane of the section (Merchán-Pérez et al., 2009 (link), 2013 (link); Kreshuk et al., 2011 (link); Blazquez-Llorca et al., 2013 (link)). Tissue preparation involves fixation in aldehydes, osmication, en bloc staining with uranyl acetate, dehydration, and embedding in Araldite. Stacks of serial images are then obtained by automated FIB/SEM (Merchán-Pérez et al., 2009 (link)). Since image segmentation, quantification, and analysis of synaptic junctions in these stacks are all labor-intensive procedures, we have developed ESPINA, a software tool that greatly facilitates and accelerates these processes (Morales et al., 2011a (link)). ESPINA makes use of the fact that presynaptic and postsynaptic densities appear as dark, electron-dense structures under the electron microscope. ESPINA uses a gray-level threshold to extract all the voxels that fit the gray levels of the synaptic junction. The resulting 3D object is irregularly-shaped and flattened, and includes both the pre- and postsynaptic densities and their outer contours (Figures 1A–H). Here we propose a method to extract the SAS, (equivalent to both the AZ and the PSD), from these reconstructed 3D volumes, based on the extraction of interior surfaces from volumetric representations.
The main difficulty when attempting to extract the SAS from actual 3D reconstructions of synaptic junctions resides in the large variability of their size and shape. For example, there are highly tortuous synaptic junctions, and others that have one or several holes (perforated synapses) that also vary in shape, size, and distribution. This variability precludes the use of the techniques currently available, so we have developed a new method to overcome the difficulties associated with SAS extraction. We propose a hybrid solution that obtains the desired result very efficiently by a combination of a deformable template surface and a distance transform method.
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Publication 2013
Aldehydes araldite Brain Dehydration Electron Microscopy Focused Ion Beam Scanning Electron Microscopy Hybrids Obstetric Labor Post-Synaptic Density Reconstructive Surgical Procedures Synapses Tissues uranyl acetate
New Zealand white rabbit hearts (n = 6) were swiftly excised after euthanasia (pentobarbital injection), Langendorff-perfused with Krebs-Henseleit solution (containing [in mM]: NaCl 118, KCl 4.75, CaCl2 2.5, NaHCO3 24.8, MgSO4 1.2, KH2PO4 1.2, glucose 11, insulin 10 U/L; pH 7.4) and after, 5 min wash of the coronary circulation, cardioplegically arrested a using high-K+ (25 mM) version of Krebs-Henseleit solution. All solutions were controlled for iso-osmolality (295–305 mOsm; Knauer AG, Berlin). Cardioplegically arrested hearts were perfusion-fixed with iso-osmotic Karnovsky’s fixative (Karnovsky, 1965 ) (2.4% sodium cacodylate, 0.75% paraformaldehyde, 0.75% glutaraldehyde; 300 mOsm). Tissue fragments were excised from the left ventricle and washed with 0.1 M sodium cacodylate, post-fixed in 1% OsO4 for 1 h, dehydrated in graded acetone, and embedded in Epon-Araldite resin. Semi-thick (275 nm) sections were placed on formvar-coated slot-grids, post stained with 2% aqueous uranyl acetate and Reynold’s lead citrate. Colloidal gold particles (15 nm) were added to both surfaces of the sections to serve as fiducial markers for tilt series alignment.
Preparations were imaged at the Boulder Laboratory for 3D Electron Microscopy of Cells (University of Colorado, Boulder, CO) using an intermediate voltage electron microscope (Tecnai TF30; FEI, Eindhoven, The Netherlands) operating at 300 kV. Images were captured on a 4 K × 4 K charge-coupled device camera (UltraScan; Gatan, Pleasanton, CA) using the SerialEM software package (Ress et al., 1999 ). For imaging, the specimen holder was tilted from +60° to −60° at 1° intervals. For dual-axis tilt series the specimen was then rotated by 90° in the X-Y plane, and another +60° to −60° tilt series was taken. The images from each tilt-series were aligned by fiducial marker tracking and back-projected to generate two single full-thickness reconstructed volumes (tomograms), which were then combined to generate a single high-resolution 3D reconstruction of the original partial cell volume (Mastronarde, 1997 (link)). Isotropic voxel size was 1.206 nm. In some instances, tomograms were computed from montaged stacks, to increase the total reconstructed volume to up to 10 × 10 μm in X-Y. Biologically meaningful resolution was approximately 4 nm in X-Y. All tomograms were processed and analysed using IMOD software (Mastronarde, 2006 (link)), which was also used to generate 3D models of relevant structures of interest (Kremer et al., 1996 (link)). Models were smoothed and meshed to obtain the final 3D representation, in which spatial relations of various cardiomyocyte sub-structures were quantified.
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Publication 2016
Biopsy samples, retrieved from our archival histology collection, were taken in the period 1981–95 from the gastric antrum and corpus of 26 subjects (15 males and 11 females, aged between 26 and 79 years) undergoing routine endoscopic and histologic examination for dyspepsia as requested by the physician in charge of the patient and with the written consent of the patient. One of the specimens from both the antrum and the corpus was processed for TEM and the pertinent semithin resin section used for diagnostic purposes together with those of routine histologic material. No specimen specifically and/or exclusively devoted to the present study was taken. The study has been approved by the Ethics Committee of Fondazione IRCCS Policlinico San Matteo (Pavia, Italy) as a reinvestigation of archival material along the same line (i.e., diagnosis of H. pylori-dependent gastritis) as for the original consensus.
The samples were fixed in 4% formaldehyde and embedded in paraffin for histologic investigation, or fixed for 4 hours with 2% formaldehyde and 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.3), followed by 1% osmium tetroxide for 1 hour, embedded in Epon-Araldite resin and processed for TEM. Paraffin sections were stained with hematoxylin-eosin, Giemsa or H. pylori immunoperoxidase [2] (link), [17] (link). Semithin (0.5 µm) resin sections were stained with toluidine blue, while ultrathin sections were stained with uranyl-lead or the immunogold procedure [2] (link), [9] (link), using antibodies against: a) H. pylori OMPs, urease, CagA and VacA, b) NOD1 receptor, c) E1A/B ligases, polyubiquitinated or mono/polyubiquitinated proteins, 20S proteasome core subunits, 19S proteasome S2 subunit, and 20S proteasome β5i subunit, d) SHP2 tyrosine phosphatase, ERK 1/2 kinases, ribosomal protein S16 and other proteins (detailed in Table S1). Anti-rabbit or anti-mouse IgG labeled with 5, 10, 15 or 20 nm gold particles (British Bio Cell, Cardiff, UK) were then used. Tests to evaluate the specificity of immunogold labeling were carried out using antibodies absorbed with excess antigen and omitting or substituting the specific antibodies in the first layer of the immunogold procedure. Positive and negative controls were obtained by parallel investigation of H. pylori cultures, epithelial cell cultures, and gastric mucosa specimens as in previous studies [2] (link), [9] (link).
ESI analyses were performed by using a LEO 912AB electron microscope as described by Pezzati et al.[53] (link). Briefly, the net phosphorus distribution was obtained by computer processing of images collected at different energy loss values according to the three-window method. The final phosphorus map (coded in pseudocolors) was then superimposed on the ultrastructural organization of the same field obtained at 250 eV (i.e., at an energy loss where most of the elements contribute to the image).
H. pylori BCF was produced as previously described [7] (link) using the well-characterized urease+/CagA+/VacA+ wild-type H. pylori strain 60190 (ATCC 49503). Briefly, bacteria were grown in Brucella broth (BD Diagnostics, Sparks, MD) supplemented with 1% Vitox (Oxoid, Basingstoke, UK) and 5% fetal bovine serum (FBS; Gibco, Grand Island, NY) for 24–36 h at 37°C under microaerobic conditions and continuous shaking. Bacteria were then removed by centrifugation and the supernatant sterilized by passage through a 0.22 µm cellulose acetate filter. Cultured cells were incubated with H. pylori BCF diluted 1∶3 in culture medium.
VacA (with a s1a/m1 vacA genotype) was purified from BCF of H. pylori 60190 strain, grown in Brucella broth containing 0.2% β-cyclodextrins (Sigma, St Louis, MO) instead of FBS, by ammonium sulphate precipitation and gel filtration chromatography in accordance with Cover et al.[54] (link). Purified VacA was then labeled with Cy5 dye, stored in melting ice and, immediately before use, alkali activated by drop-wise addition of 0.4 N NaOH [8] (link). Cultured cells were incubated with 2 µg/ml activated Cy5-VacA.
Human epithelial cell lines HeLa (ATCC CCL-2; from cervix adenocarcinoma) and AGS (ATCC CRL-1739; from gastric adenocarcinoma) were grown in DMEM supplemented with 10% FBS and 200 mM glutamine at 37°C in a humidified atmosphere of 5% CO2 in air. After washing, subconfluent cell monolayers were incubated at 37°C for 24 h under the different experimental conditions. Cells were then either fixed and processed for TEM as described above or fixed with 4% paraformaldehyde, permeabilized with 0.1% saponin, and processed for immunofluorescence as previously described [8] (link), [22] (link) using Alexa 488-labeled anti-mouse IgG (Molecular Probes, Eugene, OR) or Texas Red- or Cy5-labeled anti-rabbit IgG (Jackson Immunoresearch, West Grove, PE) as secondary antibodies. Nuclear counterstaining was made with Hoechst 33258. TCS SP2 confocal laser scanning microscope (Leica, Heidelberg, Germany) equipped with 63x oil-immersion objective was used. Ubiquitinated protein-positive (i.e., FK2-reactive) cytoplasmic spots per cell and their colocalization with VacA or proteasome were quantified using the ImageJ software (NIH, Bethesda, MD).
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Publication 2010
All experiments were conducted in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research. Nine adult mice, 6 to 8 weeks of age (three 129/SVJ, three C57BL/6 and three BALB/c) were euthanatized, and a few drops of 2% glutaralde-hyde in 80 mM sodium cacodylate buffer, 330 mOsm/kg fixative11 (link) were immediately applied to the cornea after resection of the eye lids. The whole eyes were carefully enucleated and immersed in fixative for 4 to 6 hours, to ensure proper cross-linking and preservation of the tissue.
After fixation, corneal diameter measurements were obtained on one eye per animal under a dissecting microscope (SZ60; Olympus) for increased magnification. A pair of digital calipers with sensitivity down to 0.1 mm was used to obtain measurements from all eyes. The corneal diameter was measured from limbus to limbus by using the easily visualized (at 25× magnification) corneoscleral junction to define the limit of the cornea. The corneal limbus-to-sclera transitional zone is very narrow, at approximately 0.1 mm and was for this reason not considered separately. The mouse cornea was found to be circular, not oval like the human cornea, and, therefore, the measurements do not differ in the horizontal and vertical meridians. All three measurements on the same cornea were obtained along the same meridian, and the corneal diameter measurements were performed on fixed eyes for consistency with the other measurements. The tissue-processing protocol used in the present study has shown that a fixative maintaining near physiological osmolality produces minimal tissue shrinkage, with the result that the undistorted, natural contour of the tissue is preserved.11 (link) The average corneal radius (diameter/2) was used to identify the location where the central corneal measurements were made.
The fixed corneas were bisected, and small pieces (1 × 1.5 mm) were cut from the central region. The corneal pieces were washed three times in sodium cacodylate buffer (pH 7.4) at room temperature and left for 10 minutes in each wash. Subsequently, the samples were immersed in a freshly prepared 1% solution of osmium tetroxide in 100 mM sodium cacodylate buffer for 1 hour under dim light. The samples were once again washed several times in sodium cacodylate buffer and left 10 minutes in each wash. A tissue processor (EM TP; Leica; Wetzlar, Germany) was used for the following steps: dehydration, transition, infiltration, and embedding. First, the tissue samples were dehydrated through a graded alcohol series (30%–100% in six steps) at room temperature. Next the tissue samples were infiltrated with propylene oxide. Embedding with agitation was achieved through an initial mixture of propylene oxide and Araldite resin 2:1 for 3 hours, followed by overnight immersion in a 1:1 mixture of propylene oxide and Araldite resin. Thereafter, the tissue samples were immersed in propylene oxide and Araldite resin 1:3 for 4 to 8 hours before final transfer to 100% Araldite resin overnight. The tissue samples were then oriented in embedding molds and left 12 hours for polymerization in an oven at 60°C.
An ultramicrotome (MT-7000; Research Manufacturing Co. Inc., Tucson, AZ) was used to cut thick transverse sections (0.5–1 μm). These sections were stained with 1% toluidine blue for examination with a light microscope (BX51; Olympus). For morphologic analysis, ultrathin sections were obtained and mounted on parallel bar copper grids (200MP, cat no. G200P; Electron Microscopy Sciences, Fort Washington, PA). The sections were double stained, first, in 3.5% uranyl acetate for 20 minutes at 60°C, followed by Reynold's lead citrate for 10 minutes at room temperature. The grids were examined in a transmission electron microscope (Tecnai G2 Bio Twin Spirit; FEI Co., Eindhoven, The Netherlands) and the images captured digitally.
Digital images were captured at 40× and 200× of two thick toluidine-stained corneal sections, cut from two levels of the same block separated by approximately 200 μm. Peripheral measurements were taken at the extremity of the cornea, defined histologically as immediately central (anterior) to limbal capillaries and central to the anterior edge of the trabecular meshes (Fig. 1A).
Measurements for the central cornea were taken at a distance, half the corneal diameter ±14% from the peripheral measurements where the cornea had its natural contour, had taken up stain in a uniform manner, and was free of artifacts. All measurements were made with NIH Image Software (Wayne Rasband, National Institutes of Health, Bethesda, MD; available at http://rsb.info.nih.gov/ij/index.html) in trip-licate, at the same location centrally and peripherally.
The layers of cells forming the epithelium were counted in electron micrographs taken from both the central and peripheral regions. The cells were counted in a straight line from the basement membrane to the corneal surface and did not have to display a nucleus in the plane of the section.
An unpaired Student's t-test was used to compare the two corneal sections cut from two levels of the same block but separated by approximately 200 μm, to ensure no difference throughout the tissue sample. An unpaired Student's t-test was also used to compare the average central with the average peripheral corneal measurements in three mice within the same strain. The statistical significance was set at P < 0.05.
Publication 2009

Most recents protocols related to «Araldite»

The Araldite F (100 pbw)/Aradur HY 905 (100 pbw)/flexibiliser DY 040 (10 pbw) epoxy system is used by the company ASG Superconductors S.p.A. for impregnation of magnet coils. The curing cycle was 10 h–100 °C + 48 h–135 °C, as recommended by ASG. To increase pot life for the impregnation of large coils, the Araldite F–Aradur HY 905–flexibiliser DY 040 system does not contain the accelerator DY 061 and filler that are recommended by Huntsman [31 ,32 ].
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Publication 2024
The epoxy system referred to as Araldite F is used by the company ASG Superconductors S.p.A., Genoa, Italy, for impregnation of magnet coils and consists of the bisphenol A/epichlorohydrin resin (type DGEBA) Araldite F, the carboxylic anhydride hardener Aradur HY 905, and the polyglycol flexibilizer DY 040. The resin, hardener, and flexibilizer are combined in the ratio of 100 pbw:100 pbw:10 pbw, respectively. The Araldite F/Aradur HY 905/flexibilizer DY 040 system does not contain the accelerator DY 061, and filler that is recommended by Huntsman [19 ]. The applied curing cycle comprises two plateaus 100 °C–10h and 135 °C–48 h.
The samples were cut from 3 mm or 4 mm thick pure epoxy resin plates that were produced by vacuum impregnation at the CERN polymer lab. All samples of a given material were cut from the same plate, thus eliminating uncertainties related to the sample production processes.
Sample curing inside the mold was achieved in a forced convection furnace. The stated curing temperatures are nominal furnace temperature values, and the estimated uncertainty of the plate temperature during the isothermal plateaus is ±2 °C. During heating, the nominal temperature ramp was 10 °C per hour. Sample cooling occurred by natural convection after switching off the furnace. Unlike in large coils, where temperature gradients during curing across the coil are often unavoidable, in the epoxy sample plates, temperature gradients across the plate inside its mold are negligible.
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Publication 2024
CTD101K+DY040 sample plates were produced with 10 wt.% and with 20 wt.% Araldite DY040 relative to CTD101K epoxy resin. Araldite DY040 is a solvent-free, low-viscous hot-curing polyglycol flexibiliser [27 ], which is part of the Araldite F epoxy-resin system described below. First, the CTD101K epoxy resin and hardener were mixed and degassed, and then, DY040 was added. This mix was again degassed before the accelerator was added, and a final degassing was executed. The same curing-temperature cycle as the one for the CTD101K system was applied. In the following, the CTD101K+10 wt.% DY040 epoxy system is referred to as POLAB Mix.
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Publication 2024
For transmission electron microscopy (TEM), 0.9 × 106 infected Sf9 or High Five insect cells were harvested 4 dpi (days post infection) from the multi-well plate, pelletized for 3 min at 500 × g, fixed by resuspension in 1 mL cold cacodylate buffer (60 mM, pH 7.35) containing 2% glutaraldehyde, 6 g/L paraformaldehyde (PFA), and 0.3 g/L CaCl2, followed by incubation for at least 24 h at 4 °C. At RT, the cells were then pelletized for 3 min at 900 × g and the supernatant was carefully removed. After a 30 min wash in 120 mM sodium cacodylate buffer (pH 7), cells were postfixed in 1% osmium tetroxide (in 120 mM sodium cacodylate, pH 7) for 2 h. After two additional washing steps (15 min each) in sodium cacodylate buffer, cells were dehydrated in ethanol for 2 × 15 min at each step with increasing ethanol concentrations (30–100%; 10%-steps) and two 30 min incubations with 100% ethanol at the end. At the 70% ethanol step, cells were incubated overnight. Subsequently, samples were cleared in propylene oxide in two 30 min incubations. Embedding in Araldite was done using a mixture of 10 mL Araldite M, 10 mL Araldite M hardener 964, 0.3–0.4 mL Araldite M accelerator 960 and 0.1–0.2 mL dibutyl phthalate. First the cells were incubated for 1 h in a mixture of Araldite with propylene oxide (1:2), then another hour in a 2:1 mixture. After removal of this supernatant, the pellet was left for 2 min to evaporate the rest of the propylene oxide. Finally, the pellet was carefully overlayed with 500 µL of the Araldite M mixture and left for hardening at 60 °C for 48 h. Sections were cut with a Leica Ultracut E microtome to 60–90 nm thickness and transferred onto TEM-grids (G2410C, Plano GmbH). Sections were then stained with a Leica EM AC20 for 30 min in 0.5% uranyl acetate (Ultrostain I, Leica) at 40 °C and for 7 min in 3% lead citrate (Ultrostain II, Leica) at 20 °C. Imaging was done using a JEOL JEM-1011.
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Publication 2024
The epoxies triglycidyl-para-aminophenol (TGpAP) and triglycidyl-meta-aminophenol (TGmAP) were supplied
by Huntsman Advanced Materials (as Araldite MY0510 and Araldite MY0610,
respectively). The two amines 4,4′-diaminodiphenyl sulfone
(44′DDS) and 3,3′-diaminodiphenyl sulfone (33′DDS)
were supplied by Thermo Scientific and Huntsman Advanced Materials
(as Aradur 9719-1 NL), respectively. Four cured resin formulations
were produced and are referred to as TGpAP/44′DDS,
TGpAP/33′DDS, TGmAP/44′DDS
and TGmAP/33′DDS throughout this paper.
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Publication 2024

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Glutaraldehyde is a chemical compound used as a fixative and disinfectant in various laboratory applications. It serves as a cross-linking agent, primarily used to preserve biological samples for analysis.
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