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AT 125

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Most cited protocols related to «AT 125»

1
Nab-paclitaxel plus Gemcitabine for Pancreatic Cancer
Cited 26 times
In this international, multicenter, open-label, randomized, phase 3 study, we randomly assigned eligible patients, in a 1:1 ratio, to receive a 30-to-40–minute intravenous infusion of nab-paclitaxel at a dose of 125 mg per square meter, followed by an infusion of gemcitabine according to the gemcitabine label at a dose of 1000 mg per square meter, on days 1, 8, 15, 29, 36, and 43, or to receive gemcitabine alone at a dose of 1000 mg per square meter weekly for 7 of 8 weeks (cycle 1). In subsequent cycles, all patients were administered treatment on days 1, 8, and 15 every 4 weeks.
Patients were stratified according to performance status, presence or absence of liver metastases, and geographic region. Treatment continued until disease progression or until there was an unacceptable level of adverse events. Per protocol, crossover was not allowed at any time after randomization.
Von Hoff D.D., Ervin T., Arena F.P., Chiorean E.G., Infante J., Moore M., Seay T., Tjulandin S.A., Ma W.W., Saleh M.N., Harris M., Reni M., Dowden S., Laheru D., Bahary N., Ramanathan R.K., Tabernero J., Hidalgo M., Goldstein D., Van Cutsem E., Wei X., Iglesias J, & Renschler M.F. (2013). Increased Survival in Pancreatic Cancer with nab-Paclitaxel plus Gemcitabine. The New England journal of medicine, 369(18), 1691-1703.
Publication 2013
130-nm albumin-bound paclitaxel AT 125 Disease Progression Gemcitabine Intravenous Infusion Liver Neoplasm Metastasis Patients
2
Transcriptome Analysis of Malaria Parasites
Cited 16 times
Total RNA was prepared directly from the frozen pellets of parasitized erythrocytes, where approximately 1 ml of cell pellet was lysed in 7.5 ml Trizol (GIBCO) and RNA was extracted according to the manufacturer's instructions. mRNA was isolated from total RNA preparations using the Oligotex mRNA Mini Kit (Qiagen, Valencia, CA). For the hybridization experiments, 12 μg total RNA was used for first-strand cDNA synthesis as follows: RNA was mixed with a mixture of random hexamer (pdN6) oligonucelotides and oligo-(dT20) at final concentration 125 μg/μl for each oligonucleotide. The mixture was heated to 70°C for 10 min and then incubated on ice for 10 min. Reverse transcription was started by adding dNTPs to a final concentration of 1 mM dATP and 500 μM each: dCTP, dGTP, dTTP and 5-(3-aminoallyl)-2'-deoxyuridine-5'-triophosphate, (aa-dUTP) (Sigma), with 150 units of StrataScript (Stratagene, La Jolla, CA). The reaction was carried out at 42°C for 120 min and the residual RNA was hydrolyzed with 0.1 mM EDTA and 0.2 M NaOH at 65°C for 15 min. The resulting aa-dUTP-containing cDNA was coupled to CyScribe Cy3 or Cy5 (Amersham, Piscataway, NJ) monofunctional dye in the presence of 0.1 M NaHCO3 pH 9.0. Coupling reactions were incubated for a minimum of 1 h at room temperature. The labeled product was purified using QIAquick PCR purification system (Qiagen). Hybridizations and final washing procedures were carried out as described [9 (link)] with slight modifications. Briefly, the hybridization medium contained 3 × SSC, 1.5 μg/μl poly(A) DNA (Pharmacia Biotech, Uppsala), and 0.5% SDS. Hybridizations were incubated at 65°C for 8-16 h. Arrays were washed in 2 × SSC/0.2% SDS and then 0.1 × SSC at room temperature. The microarrays were scanned with a GenePix 4000B scanner and the images analyzed using GenePix Pro 3.0 software (Axon Instruments, Union City, CA). Subsequently, the data were normalized using the AMAD microarray database and subjected to the cluster analysis using the CLUSTER and TREEVIEW software, as described [53 (link)]. For the CLUSTER analysis, low-quality features and features with a signal level less than fivefold the background were filtered from the initial raw data set, yielding 4,737 elements. Subsequently, features with an arbitrary twofold fluorescence signal difference in at least four experiments were considered. All programs and microarray-related protocols are available online [55 ].
Bozdech Z., Zhu J., Joachimiak M.P., Cohen F.E., Pulliam B, & DeRisi J.L. (2003). Expression profiling of the schizont and trophozoite stages of Plasmodium falciparum with a long-oligonucleotide microarray. Genome Biology, 4(2), R9.
Publication 2003
2'-deoxycytidine 5'-triphosphate Acid Hybridizations, Nucleic Anabolism AT 125 Axon Bicarbonate, Sodium Cells deoxyguanosine triphosphate Deoxyuridine deoxyuridine triphosphate DNA, Complementary Edetic Acid Erythrocytes Fluorescence Freezing Microarray Analysis Oligonucleotides Pellets, Drug Poly A Reverse Transcription RNA, Messenger thymidine 5'-triphosphate trizol
3
Analyzing Single-Cell Transcriptomic Profiles of Microglia
Cited 13 times

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Publication 2018
AT 125 Brain Cells DNA Replication Fingers Gene Expression Genes Genome Injuries Microglia Mus Neurons White Matter
4
ChIP-seq and FAIRE-seq in ESCs
Cited 13 times

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Publication 2014
Antibodies AT 125 Cells Chromatin Chromatin Immunoprecipitation Sequencing DNA Chips Formaldehyde G-substrate Genome Glycine Immunoglobulins Immunoprecipitation, Chromatin
5
Characterizing Neuronal Membrane Properties
Cited 13 times
To characterize the intrinsic membrane properties of neurons, hyperpolarizing and depolarizing current steps of 500-ms duration were applied in 10-pA increments at 0.125 Hz as described previously15 (link),19 (link). Two parameters were measured: resting membrane potential (Vrest), defined as the stable membrane potential reached after break in with no holding current applied, and input resistance (Rm), defined as the slope of the linear regression of the steady-state I-V curve. We used the value of the spike threshold as the EPSP amplitude for the neurons that elicited spikes in Figure 1.
Lee S., Kruglikov I., Huang Z.J., Fishell G, & Rudy B. (2013). A disinhibitory circuit mediates motor integration in the somatosensory cortex. Nature neuroscience, 16(11), 1662-1670.
Publication 2013
AT 125 Excitatory Postsynaptic Potentials Membrane Potentials Neurons Resting Potentials Tissue, Membrane

Most recents protocols related to «AT 125»

1
Schistosoma mansoni Carm1 RNAi Assay
The Smcarm1 coding sequence was obtained from the GeneDB database.2 The T7 promoter sequence was added to the 5′-end of primers designed to amplify a template of 569 bp for double-stranded RNAs (dsRNAs) synthesis. A fragment of 360 bp from the green fluorescent protein (GFP) cloned in the pCRII plasmid vector, was used as non-schistosome RNA interference (RNAi) control. The PCR amplified fragments were purified with the QIAquick Gel Extraction KIT (QIAGEN) and used for dsRNA synthesis using the T7 RiboMAX Express RNAi System kit (Promega) according to the supplier’s protocol; except for the time of reactions which was changed to 16 h. DsRNAs annealing and integrity were verified in 1% agarose gel electrophoresis, and the quantification was estimated in a Nanodrop Spectrometer ND-1000 (Thermo Fischer Scientific).
Approximately 2,000 schistosomula were exposed to 100 nM of dsRNAs (Smcarm1 or GFP), immediately after mechanical transformation, in 24-well plate and incubated for 7 days at 37°C, 5% CO2, and 95% humidity with 2 ml of GMEM supplemented as previously mentioned.
Eight adult worms (males and females, separately) were placed in 100 μl of RPMI 1640 medium with 25 μg of dsRNA. The worms were electroporated with specific Smcarm1-dsRNA or unspecific GFP-dsRNA into 4 mm cuvettes at 125 V for 20 ms and cultivated in 24-well plate with 1 ml RPMI 1640 medium supplemented with 10% heat-inactivated FBS and 2% penicillin/streptomycin. Similarly, to count the number of eggs laid, eight worm pairs were electroporated and cultured in six-well plate and the medium was changed daily.
Coelho F.S., Gava S.G., Andrade L.F., Geraldo J.A., Tavares N.C., Lunkes F.M., Neves R.H., Machado-Silva J.R., Pierce R.J., Oliveira G, & Mourão M.M. (2023). Schistosoma mansoni coactivator associated arginine methyltransferase 1 (SmCARM1) effect on parasite reproduction. Frontiers in Microbiology, 14, 1079855.
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Publication 2023
Adult Anabolism AT 125 Cloning Vectors Eggs Electrophoresis, Agar Gel Females Green Fluorescent Proteins Helminths Humidity Males neuronectin Oligonucleotide Primers Open Reading Frames Penicillins Plasmids Promega RNA, Double-Stranded RNA Interference Schistosoma Streptomycin
2
Monoclonal Antibody Production in Expi293 Cells

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Publication 2023
Antigens AT 125 Bacteria beta-Globins Cells Centrifugation Cloning Vectors Culture Media Escherichia coli Humidity Light LY-COV555 Monoclonal Antibodies Plasmids Transfection
3
Synthesis and Characterization of Specialty Chemicals
The compound 2-fluoro 5-nitrotoluene (99%), 2-chloro-5-nitrotrifluoromethyl (98%), 1-chloro-4-nitrobenzene (99.5%), bisphenol A (99%), 4,4′-(hexafluoroisopropylidene)diphthalic anhydride(6FDA, 99%), DB18-CE-6 (98%), were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd (China). Dianhydride was dried in vacuum at 125 °C for 24 h before samples preparation. All other reagents were obtained from Sinopharm Chemical Reagent Co., Ltd (China) and used as received.
Li H., Wang X., Gong Y., Zhao H., Liu Z., Tao L., Peng Y., Ma K., Hu Z, & Dastan D. (2023). Polyimide/crown ether composite film with low dielectric constant and low dielectric loss for high signal transmission. RSC Advances, 13(11), 7585-7596.
Publication 2023
4-chloronitrobenzene Anhydrides AT 125 bisphenol A Vacuum
4
Genome-wide Chromatin Profiling by ChIP-seq and ATAC-seq
Chromatin immunoprecipitation (ChIP) and Assay for Transposase-Accessible Chromatin (ATAC) sequencing was performed as previously described36 (link). For ChIP-seq a total of 10 × 107 cells were crosslinked with 1% formaldehyde while shaking for 7 min at room temperature, quenched with 125 mM glycine, lysed and sonicated with the S2 Covaris for 30 min to obtain 200–300 bp long fragments. Chromatin fragments were immunoprecipitated overnight using 1 µg antibody of SOX11-PAb antibody (custom made by Absea biotechnology, China) and 20 µl Protein A UltraLink® Resin (Thermo Scientific, #53139) beads per 10 × 107 cells. Reverse crosslinking was done at 65 °C for 15 h and chromatin was resuspended in TE-buffer, incubated for 2 h at 37 °C with 0.2 mg/ml RNase and followed by an incubation of 2 h at 55 °C with 0.2 mg/ml proteinase K. DNA was isolated using 400 µl phenol:chloroform:isoamylalcohol (P:C:IA) in phase lock gel tubes (5Prime). Upon centrifugation, the aqueous layer was transferred to a new tube with 200 mM NaCl, 30 µg glycogen and 800 µl 100% ethanol, and incubated for 30 min at −20 °C. Upon centrifugation, the pellet was washed with 80% Ethanol and resuspended in RNase/DNase free water. DNA concentration was measured using the Qubit® dsDNA HS Assay Kit. Library prep was done using the NEBNExt Ultra DNA library Prep Kit for Illumina (E7370S) with 500 ng starting material and using 8 PCR cycles according to the manufacturer’s instructions. For ATAC-seq, 50,000 cells were lysed and fragmented using digitonin and Tn5 transposase. The transposed DNA fragments were amplified and purified using Agencourt AMPure XP beads (Beckman Coulter). ChIP and ATAC library concentrations were measured with the Illumina Kapa Library quantification kit (Roche #07960140001) and libraries were sequenced on the NextSeq 500 (Illumina) using the Nextseq 500 High Output kit V2 75 cycles single-end (Illumina).
Decaesteker B., Louwagie A., Loontiens S., De Vloed F., Bekaert S.L., Roels J., Vanhauwaert S., De Brouwer S., Sanders E., Berezovskaya A., Denecker G., D’haene E., Van Haver S., Van Loocke W., Van Dorpe J., Creytens D., Van Roy N., Pieters T., Van Neste C., Fischer M., Van Vlierberghe P., Roberts S.S., Schulte J., Ek S., Versteeg R., Koster J., van Nes J., Zimmerman M., De Preter K, & Speleman F. (2023). SOX11 regulates SWI/SNF complex components as member of the adrenergic neuroblastoma core regulatory circuitry. Nature Communications, 14, 1267.
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Publication 2023
AT 125 ATAC-Seq Biological Assay Buffers Cells Centrifugation Chloroform Chromatin Deoxyribonucleases Digitonin DNA, Double-Stranded DNA Library Ethanol Formaldehyde Glycine Glycogen Immunoglobulins Immunoprecipitation, Chromatin Phenol proteinase C Resins, Plant ribonuclease C Ribonucleases Sodium Chloride SOX11 protein, human Staphylococcal Protein A Tn5 transposase Transposase XCL1 protein, human
5
Histological and Immunohistochemical Analysis
Sections for histological analysis were deparaffinized in Clear Care Plus solution (xylene substitute 1276; Kaltek, Padua, Italy), rehydrated, and stained in hematoxylin and eosin (H&E) solution. All observations and photography were performed using an Olympus BX41 microscope equipped with a DP70 digital camera (Olympus, Tokyo, Japan). Sections for immunohistochemistry were deparaffinized in xylene, dehydrated in graded alcohols, and rinsed with phosphate-buffered saline (PBS, pH 7.4, P3813; Sigma-Aldrich). For antigen retrieval, they were incubated in a decloaking chamber with 10-mM sodium citrate buffer with 0.05% Tween 20 (pH 6.0) at 125°C for 10 minutes. The slides were then cooled down to room temperature and blocked for 1 hour with 10% normal donkey serum in PBS solution (pH 7.4, P3813; Sigma-Aldrich) and 0.1% Triton X-100 at room temperature. Subsequently, sections were incubated overnight at 4°C in a humid chamber with the polyclonal antibody C-Nap1 (E-19) 1:100 (sc74349; Santa Cruz Biotechnology, Dallas, TX). After they were washed with PBS, the slides were incubated with DyLight 488 Donkey anti-Rabbit (1:250; Jackson Immuno Research Laboratories, West Grove, PA). Sections were washed in double distilled water, and nuclei were counterstained with 4′,6-diamidino-2-phenylindole (71-03-01; SeraCare, Mildord, MA). The slides then were mounted with VECTASHIELD mounting medium (H1000; Vector Laboratories, Newark, CA). An Olympus BX41 microscope equipped with a DP70 digital camera was used for observations and photography.
Abu-Diab A., Gopalakrishnan P., Matsevich C., de Jong M., Obolensky A., Khalaileh A., Salameh M., Ejzenberg A., Gross M., Banin E., Sharon D, & Khateb S. (2023). Homozygous Knockout of Cep250 Leads to a Relatively Late-Onset Retinal Degeneration and Sensorineural Hearing Loss in Mice. Translational Vision Science & Technology, 12(3), 3.
Publication 2023
Antigens AT 125 Buffers Cell Nucleus Cloning Vectors Eosin Equus asinus Ethanol Fingers Immunoglobulins Immunohistochemistry Microscopy nucleic acid probe 1 Phosphates Rabbits Saline Solution Serum Sodium Citrate Triton X-100 Tween 20 Xylene

Top products related to «AT 125»

1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
2
Expi293f cells by Thermo Fisher Scientific
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Expi293F cells are a suspension-adapted mammalian cell line derived from HEK293F cells. They are designed for high-level recombinant protein expression in biopharmaceutical and biotechnology applications.
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RPMI 1640 is a common cell culture medium used for the in vitro cultivation of a variety of cells, including human and animal cells. It provides a balanced salt solution and a source of essential nutrients and growth factors to support cell growth and proliferation.
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5
Expi293 expression medium by Thermo Fisher Scientific
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Expi293 Expression Medium is a high-performance cell culture medium designed for the transient expression of recombinant proteins in HEK293 cells. It supports efficient transfection and high-level protein production in suspension culture.
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L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
9
Decloaking chamber by Biocare Medical
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The Decloaking Chamber is a laboratory equipment designed to aid in the process of antigen retrieval. It is used to expose antigens in fixed tissue samples, which is a crucial step in immunohistochemical and immunofluorescent staining procedures. The chamber provides a controlled environment for the heating and cooling of samples, allowing for the effective retrieval and subsequent detection of target antigens.
10
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.

More about "AT 125"

AT 125 is a research topic that involves the optimization and streamlining of various protocols and techniques used in scientific research.
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The advanced comparison tools offered by PubCompare.ai can enhance reproducibility and drive research forward in the AT 125 domain.
Researchers can leverage these tools to find and optimize protocols related to cell culture techniques, such as the use of FBS (Fetal Bovine Serum), Expi293F cells, and RPMI 1640 medium.
Additionally, the platform can assist in the optimization of protocols involving chemical treatments, like the use of formaldehyde, as well as the preparation of expression media, such as Expi293 Expression Medium, which often includes L-glutamine.
The identification and comparison of sequencing techniques, including the use of HiSeq 2500 instruments, can also be facilitated by PubCompare.ai.
Furthermore, the platform can help researchers optimize protocols related to antibiotics, such as the use of Penicillin, as well as specialized equipment like the Decloaking Chamber.
By incorporating these diverse aspects of AT 125 research, PubCompare.ai can provide a comprehensive solution for researchers to streamline their work, enhance reproducibility, and drive their research forward in this important field.
The platform's expertise and advanced tools can unlock the full potential of AT 125 research, leading to more efficient and impactful scientific discoveries.