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Bakelite
Bakelite
Bakelite is a thermoset plastic material made from phenol and formaldehyde.
It was the first synthetic plastic to be commercially produced and has a wide range of applications, including electrical insulation, telephone handsets, and decorative objects.
Bakelite is known for its durability, heat resistance, and electrical insulating properties.
It has been used in the manufacture of a variety of products since its invention in the early 20th century.
Researchers can use PubCompare.ai's AI-driven platform to easily locate and compare protocols for Bakelite from literature, pre-prints, and patents, helping to identify the most reproducible and accurate methods and enhacing the efficieny of their Bakleite research.
It was the first synthetic plastic to be commercially produced and has a wide range of applications, including electrical insulation, telephone handsets, and decorative objects.
Bakelite is known for its durability, heat resistance, and electrical insulating properties.
It has been used in the manufacture of a variety of products since its invention in the early 20th century.
Researchers can use PubCompare.ai's AI-driven platform to easily locate and compare protocols for Bakelite from literature, pre-prints, and patents, helping to identify the most reproducible and accurate methods and enhacing the efficieny of their Bakleite research.
Most cited protocols related to «Bakelite»
2-Mercaptoethanol
Amino Acids, Essential
bakelite
Cells
Culture Media
Embryo
Fibroblast Growth Factor 2
Gelatins
Growth Factor
Heparin
Hyperostosis, Diffuse Idiopathic Skeletal
Immunoglobulins
inhibitors
Kidney Cortex
LIF protein, human
Lipids
matrigel
Methylcellulose
Mus
neuronectin
Organoids
Oxygen
Permeability
Pyruvate
Serum
Sodium
STAT3 Protein
Transforming Growth Factor beta
Vitamin A
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An automated protein purification system (ED-01; GP BioSciences Ltd., Yokohama, Japan) was used to immunoprecipitate M2BP from serum specimens. In brief, sera (2 μl) were diluted 10-fold with PBS/0.2%(w/v) SDS, heated at 95°C for 20 min, mixed with 10 μl of Triton X-100 in TBS (TBSTx) and injected into a 96-well SUMILON microtiter plate (Sumitomo Bakelite Co., Ltd., Tokyo, Japan). The plate and working reagents, including biotinylated anti-M2BP antibody (10 ng/μl), streptavidin-coated magnetic beads, washing buffer (1% TBSTx) and elution buffer (TBS containing 0.2% SDS), were loaded into the system. This generated 110 μl of purified M2BPs per serum sample (96 samples in 3.5 h).
Antibodies, Anti-Idiotypic
bakelite
Buffers
Proteins
Serum
Streptavidin
Triton X-100
For SFEBq culture, hESCs were dissociated to single cells in TrypLE Express (Invitrogen) containing 0.05 mg ml−1 DNase I (Roche) and 10 μM Y-27632 (TOCRIS), and quickly reaggregated using low-cell-adhesion 96-well plates with V-bottomed conical wells (Sumilon PrimeSurface plate; Sumitomo Bakelite) in differentiation medium (9,000 cells per well, 100 μl) containing 20 μM Y-27632 under 5% CO2. The differentiation medium was Glasgow-MEM (Invitrogen) supplemented with 20% (vol/vol) KSR, 0.1 mM nonessential amino acids, 1 mM pyruvate, 0.1 mM 2-mercaptoethanol, 100 U ml−1 penicillin and 100 μg ml−1 streptomycin. Defining the day on which the SFEBq culture was started as day 0, 3 μM IWR1e (Wnt inhibitor; Calbiochem) and 5 μM SB431542 (TGFβ inhibitor; TOCRIS) were added to culture from day 0 to day 18. The culture method after day 18 was as follows.
For neocortical induction (condition 1,Fig. 1 )12 (link), at day 18, the floating aggregates were transferred from a 96-well plate to a 9-cm Petri dish (non-cell adhesive) and further cultured in suspension using DMEM/F-12, GlutaMAX(TM) (Gibco) medium supplemented with 1% N-2 supplement (Invitrogen), 1% Chemically Defined Lipid Concentrate (Invitrogen), 0.25 mg ml−1 fungizone (Gibco), 100 U ml−1 penicillin and 100 μg ml−1 streptomycin under 40% O2/5% CO2 conditions.
For choroid plexus-like tissue generation (condition 2,Fig. 1 ), from day 18, the floating aggregates were transferred to a Petri dish (non- cell adhesive) and further cultured in suspension using DMEM/F-12 GlutaMAX(TM) (Gibco) supplemented with 1% N-2 supplement (Invitrogen), 1% Chemically Defined Lipid Concentrate (Invitrogen), fetal bovine serum (FBS; 10% vol/vol), 3 μM CHIR 99021 (GSK3 inhibitor; Stemgent) and 0.5 nM BMP4 (R&D) under 40% O2/5% CO2 conditions. Medium was changed once every 3 days.
For medial pallium induction (condition 3,Fig. 2 ), the culture condition from day 0 to day 21 was the same as the choroid plexus induction condition. On day 21, the medium was changed to DMEM/F-12 GlutaMAX(TM) supplemented with 1% N-2 supplement, 1% Chemically Defined Lipid Concentrate, and FBS (10% vol/vol). Medium was changed once every 3 days.
For long-term culture of medial pallium tissues, the aggregates were cultured under the following conditions. On day 27, the base medium was changed to Neurobasal medium (Gibco) supplemented with 2% B-27 supplement without vitamine A (Gibco), 2 mML -glutamine, 100 U ml−1 penicillin, 100 μg ml−1 streptomycin, 0.25 mg ml−1 fungizone (Gibco) and FBS (10% vol/vol). At day 35, the aggregates were cut into half-size with fine forceps under a dissecting microscope for the prevention of cell death in the central portions, and were cultured using a Lumox dish (SARSTEDT; high-O2 penetration) after day 50. Medium change was performed once every 3 days.
For neocortical induction (condition 1,
For choroid plexus-like tissue generation (condition 2,
For medial pallium induction (condition 3,
For long-term culture of medial pallium tissues, the aggregates were cultured under the following conditions. On day 27, the base medium was changed to Neurobasal medium (Gibco) supplemented with 2% B-27 supplement without vitamine A (Gibco), 2 mM
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2-Mercaptoethanol
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide
Amino Acids
bakelite
Bone Morphogenetic Protein 4
Cell Adhesion
Cell Death
Cells
Chir 99021
Cortex, Cerebral
Culture Techniques
Deoxyribonuclease I
Dietary Supplements
Forceps
Fungizone
Glutamine
Glycogen Synthase Kinase 3
Human Embryonic Stem Cells
Hyperostosis, Diffuse Idiopathic Skeletal
Lipids
Microscopy
Penicillins
Plexus, Chorioid
Pyruvate
Streptomycin
Tissues
Transforming Growth Factor beta
Vitamin A
Y 27632
Most recents protocols related to «Bakelite»
In the fabrication of the nopal powder-based TENG, the outer surfaces of two bakelite plates (52.2 mm × 52.2 mm × 1.5 mm) are covered with a copper tape (62.2 mm × 52.2 mm × 66 μm) as shown in Fig. 2 (a,d). A section (10 mm × 52.2 mm × 66 μm) of the copper tape exceeds the bakelite plate, which is adhered to the underside of this plate. In addition, a polyimide film adhesive tape (52.2 mm × 52.2 mm × 60 μm) was adhered to the copper electrode located on the upper plate (Fig. 2 (b)). Next, a 0.644 mm-diameter tinned copper wire was soldered to the section of the copper tape adhered underside of the plate (Fig. 2 (c)). Furthermore, the nopal powder was adhered to the copper electrode placed on the lower plate (Fig. 2 (e)). To adhere the nopal powder to the copper electrode/bakelite plate, a controlled layer of white adhesive was applied to the outer surface of the copper electrode using the spin-coating process at a rotation speed of 1550 rpm for 15 s. The nopal powder was evenly spread across the glue-coated surface of the copper electrode. In the following step, we collocated the nopal powder adhered to a copper tape/bakelite plate to air dry for 24 h at ambient room temperature. Subsequently, the residues of nopal powder were removed using an air compressor. After, we solder a tinned copper wire to the electrode on the lower plate (Fig. 2 (f)). For the structural frame of the nanogenerator, a recycled PET sheet (106.79 mm × 52.20 mm × 0.30 mm) was used as shown in Fig. 2 (g). Fig. 2 (h) depicts both bakelite plates glued to a recycled PET sheet, forming the complete structure of the nopal powder-based TENG. Fig. 2 (i)) illustrates the two triboelectric layers and bakelite plates glued to the PET sheet to develop the nopal powder-based TENG. In this nanogenerator, the polyimide film acts as an electronegative triboelectric layer, while nopal powder operates as the electropositive triboelectric layer. Each proposed TENG has a mass close to 25 g, considering its bakelite plates, electrodes, both triboelectric layers and PET sheet.Fig. 2 ![]()
Fabrication of the nopal powder-based TENG. (a–c) Stages to develop the upper triboelectric layer with polyimide tape. (d–f) Stages to fabricate the bottom triboelectric layer with nopal powder. (g–i) Assembly of both triboelectric layers in a recycled PET sheet to obtain the nopal powder-based TENG.
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Softwood kraft lignin (KL)
Indulin AT was supplied by Ingevity, and soda lignin (SL) Protobind
1000 was purchased from Tanovis AG. SL (average particle size 1–10
μm) was used as received, while KL (average particle size 10–100
μm) was subjected to ball milling prior to use in order to achieve
for both lignins similar granulometry [scanning electron microscopy
(SEM) images are reported inFigure S1 in
the Supporting Information].
For the functionalization reaction
of both lignins, SAn, 1-methylimidazole (1-MI) and tetrahydrofuran
(THF) were used as received from Sigma-Aldrich. The commercial PF
resin formulation employed was provided by Camfart S.r.l. and was
composed by the novolac Bakelite PF SM 1112 [containing phenols and
8.5–9.5 wt % hexamethylenetetramine (HMTA), in powder form]
and the liquid resole Bakelite PF 2770, consisting of phenols and
<1 wt % of free formaldehyde.
Indulin AT was supplied by Ingevity, and soda lignin (SL) Protobind
1000 was purchased from Tanovis AG. SL (average particle size 1–10
μm) was used as received, while KL (average particle size 10–100
μm) was subjected to ball milling prior to use in order to achieve
for both lignins similar granulometry [scanning electron microscopy
(SEM) images are reported in
the Supporting Information].
For the functionalization reaction
of both lignins, SAn, 1-methylimidazole (1-MI) and tetrahydrofuran
(THF) were used as received from Sigma-Aldrich. The commercial PF
resin formulation employed was provided by Camfart S.r.l. and was
composed by the novolac Bakelite PF SM 1112 [containing phenols and
8.5–9.5 wt % hexamethylenetetramine (HMTA), in powder form]
and the liquid resole Bakelite PF 2770, consisting of phenols and
<1 wt % of free formaldehyde.
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MDCK
cells were dispersed on a 96-well plate at a U-shaped low protein
adhesion surface (Prime Surface, Sumitomo Bakelite Co. Ltd., Tokyo,
Japan) at a concentration of 3000 cells/well and cultured for 4 days
in DMEM containing 10% FBS at 37 °C under 5% CO2 conditions.
cells were dispersed on a 96-well plate at a U-shaped low protein
adhesion surface (Prime Surface, Sumitomo Bakelite Co. Ltd., Tokyo,
Japan) at a concentration of 3000 cells/well and cultured for 4 days
in DMEM containing 10% FBS at 37 °C under 5% CO2 conditions.
The synovial cells were cultured following previously established protocols.36 (link) The synovium was digested using a solution of 3 mg/mL collagenase (Sigma–Aldrich Co., LLC, Merck KGaA, Darmstadt, Germany) at 37°C. After 3 h, the digested cells were filtered through a 70 μm cell strainer (Greiner Bio-One GmbH, Frickenhausen, Germany) and washed with phosphate-buffered saline (Thermo Fisher Scientific, MA, USA). The cells were cultured in a 10 cm dish (Nunc, Thermo Fisher Scientific) using α-minimum essential medium (α-MEM; Thermo Fisher Scientific) supplemented with 1% antibiotic–antimycotic (Thermo Fisher Scientific) and 10% fetal bovine serum (Thermo Fisher Scientific) in a cell culture incubator (Astec Co., Ltd., Fukuoka, Japan) at 37°C in a 5% CO2 atmosphere. To evaluate the culture vessel, cells were seeded in six-well plates (BD Falcon, NJ, USA), a dish with a 15 cm diameter (Corning by Thermo Fisher Scientific), T75 flask (Sumitomo Bakelite Co., Ltd., Tokyo, Japan), and T225 flask (Sumitomo Bakelite Co.). Each cell was ruled out for mycoplasma infection by PCR.
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Cell concentrations was measured using Hemocytometer Standard Specification (Improved Neubauer) (HIRSCHMANN LAB, Baden-Württemberg, Germany). 3 ml of PBS (D-PBS(-) without Ca and Mg, #14249–95, Nacalai Tesque, Kyoto, Japan) containing 0.25–1.0×104 cells were added onto a dish (PrimeSurface Dish 35, #MS-9035X, Sumitomo Bakelite, Tokyo, Japan).
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Top products related to «Bakelite»
Sourced in Japan
PrimeSurface is a laboratory equipment product from Sumitomo Bakelite. It is a surface treatment device designed to enhance the wettability and adhesion properties of materials.
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
Sourced in Japan
Sumilon is a laboratory equipment product manufactured by Sumitomo Bakelite. It is designed for general laboratory use.
Sourced in Japan
PrimeSurface 96U is a 96-well microplate designed for various laboratory applications. It features a U-shaped well configuration, providing a larger surface area compared to standard flat-bottom plates. The product is made of high-quality materials to ensure durability and consistent performance in laboratory settings.
Sourced in Japan
PrimeSurface 96U plate is a laboratory equipment product designed for cell culture applications. It features a 96-well format and is made with a proprietary surface treatment to promote cell attachment and growth. The core function of this product is to provide a standardized and consistent platform for various cell-based experiments and assays.
Sourced in Japan, United Kingdom, Germany
The GIF-Q260J is a laboratory equipment product from Olympus. It is designed to perform a core function, but the specific details of its intended use are not available in this concise, unbiased, and factual description.
Sourced in United States, China, Germany, United Kingdom, Canada, Japan, France, Netherlands, Montenegro, Switzerland, Austria, Australia, Colombia, Spain, Morocco, India, Azerbaijan
Matrigel is a complex mixture of extracellular matrix proteins derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is widely used as a basement membrane matrix to support the growth, differentiation, and morphogenesis of various cell types in cell culture applications.
Sourced in Japan
BlotGlyco is a lab equipment product developed by Sumitomo Bakelite. It is designed for the analysis and detection of glycoproteins. The core function of BlotGlyco is to facilitate the separation and visualization of glycosylated proteins using electrophoretic techniques.
Sourced in United States, Germany, United Kingdom, Japan, Italy, China, Macao, Switzerland, France, Canada, Sao Tome and Principe, Spain, Australia, Ireland, Poland, Belgium, Denmark, India, Sweden, Israel, Austria, Brazil, Czechia, Netherlands, Portugal, Norway, Holy See (Vatican City State), New Zealand, Hungary, Senegal, Argentina, Thailand, Singapore, Ukraine, Mexico
FBS, or Fetal Bovine Serum, is a commonly used cell culture supplement. It is derived from the blood of bovine fetuses and provides essential growth factors, hormones, and other nutrients to support the growth and proliferation of a wide range of cell types in vitro.
Sourced in United States, United Kingdom, Germany, Japan, Canada, China, Italy, France, Switzerland, Spain, Israel, Australia, Austria, Poland, Denmark, Palestine, State of
B27 supplement is a serum-free and animal component-free cell culture supplement developed by Thermo Fisher Scientific. It is designed to promote the growth and survival of diverse cell types, including neurons, embryonic stem cells, and other sensitive cell lines. The core function of B27 supplement is to provide a defined, optimized combination of vitamins, antioxidants, and other essential components to support cell culture applications.