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Benzaldehyde

Benzaldehyde is a colorless, aromatic liquid compound with the chemical formula C6H5CHO.
It is a widely used industrial chemical, serving as a precursor for various organic synthesees and as a flavoring agent in food and cosmetics.
Benzaldehyde has a distinctive almond-like odor and is naturally occuring in many fruits and plants.
It is an important intermediate in the production of pharmaceuticals, dyes, and other chemicals.
Researchers can optimize their Benzaldehyde studies using PubCompare.ai, an AI-driven platform that helps locate reliable protocols from literature, preprints, and patents, while providing insighful comparisons to identify the best products and procedures.
This innovative solution enhances reproducibility and accueracy in Benzaldehyde research.

Most cited protocols related to «Benzaldehyde»

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Publication 2008
Acclimatization Adult Agar Animals Azides Bacteria benzaldehyde Biological Assay Buffers Cells Chemotaxis Diacetyl Disgust Edema Epiphyseal Cartilage Ethanol Fingers Heat-Shock Response Laser Ablation Magnesium Chloride methylethyl ketone Odors Pharmaceutical Preparations Phosphates Reading Frames Sodium Azide Solvents Strains ST Segment Elevation Myocardial Infarction Sulfate, Magnesium Sulfoxide, Dimethyl Technique, Dilution
Approval for the collection of the cancer specimens and for the use of the animal model was obtained through the appropriate review boards. The University of Michigan's guide for the care and use of laboratory animals was followed. Samples of HNSCC cancer were obtained from subjects undergoing surgical resection or biopsy of their tumor. Specimens were immediately transported to the lab in DMEM containing 10% Fetal Bovine Serum on ice. Single cell suspensions were created.
Specimens were cut into small fragments with sterile scissors and minced with a sterile scalpel, rinsed with Hanks' Balanced Salt Solution (HBSS) containing 2% heat inactivated calf serum (HICS) and centrifuged for 5 min at 1000 rpm. The resulting tissue specimen was placed in a solution of DMEM F-12 containing 300u/mL collagenase and 100u/mL hyaluronidase (Stem Cell Technologies). The mixture was incubated at 37°C mixing to dissociate cells. The digestion was arrested with the addition of Fetal Bovine Serum and the cells were filtered through 40-μm nylon sieve. The cells were washed twice with HBSS/2% HICS and stained for flow cytometry as described below.
Aldehyde dehydrogenase activity was identified in the cancer cells using the ALDEFLUOR® substrate per the manufacturer's protocol (STEMCO Biomedical). Specimens that were analyzed for ALDH activity were counter stained with anti-CD44 (allophycocyanin (APC) conjugated: BD Pharmingen) at the appropriate dilution. Cells of other lineages were identified and removed using markers anti-CD2, CD3, CD10, CD16, CD18, (CyChrome (Cy) conjugated: BD Pharmingen) that are not expressed on the tumor cells. Non-viable cells were eliminated using DAPI (BD Pharmingen). During flow cytometry analysis other lineage cells and the DAPI stained dead cells were eliminated by gating. The specific flow gates for ALDH positive cells were set using a control sample of the isolated tumor cells in which ALDH activity was inhibited with diethylamino-benzaldehyde (DEAB). Subsequent flow cytometry runs were used to identify populations of cells with high aldehyde dehydrogenase activity (ALDHhigh) and those that express the surface marker CD44 (CD44+). When cell numbers allowed measurements assessed the percent of each population present; ALDHhigh, ALDHlow, CD44+ and CD44-. Overlap between these populations was assessed to identify populations of cells with the characteristics CD44+ ALDHhigh, CD44+ALDHlow, CD44-ALDHhigh and CD44- ALDHlow in HNSCC.
HNSCC subpopulations of interest were collected based on their ALDH expression. Subpopulations of ALDHhigh and ALDHlow cells were injected subcutaneously into NOD/SCID mice and evaluated for their tumorigenic potential. When sufficient numbers were available the cells were serially diluted prior to injection. The cells were mixed with Matrigel Basement Membrane Matrix (BD Pharmingen) solution to form a final volume of 200 μL. Injection sites were sealed with a liquid skin adhesive. The animals were assessed for tumor growth.
The tumorgenicity of the injected cell populations was evaluated by evidence of tumor growth in NOD/SCID mice and by histology. When the number of cells allowed for serial dilutions to be injected we determined the minimum number of cells required for each population to produce a tumor in the NOD/SCID mouse. The resultant tumors were assessed by flow cytometric and histologic analysis for tumor heterogeneity and proportion of cells with ALDH activity.
Publication 2010
Nutlin-3a, an inhibitor of MDM2 that is reported to bind directly to MDM2, release, stabilize and activate p53 10 (link), was acquired from Cayman Chemical Company. Brefeldin A, N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone(zVAD-fmk) and other chemicals were purchased from Sigma Aldrich or Fisher Scientific or were synthesized according to literature procedures. The synthesis of Arylquin 1, which utilized 4-(N,N-dimethylamino)-2-aminobenzaldehyde in a Friedländer condensation with 2-fluorophenylacetontrile 15 , and other heterocyclic families is described in Supplementary Note. The condensation of 2-amino-4-(N,N-dimethylamino)benzaldehyde with 2-(2-fluorophenyl)acetyl chloride secured 7-(dimethylamino)-3-(2-fluorophenyl)quinolin-2(1H)-one, and treatment with Lawesson's reagent 16 provided 7-(dimethylamino)-3-(2-fluorophenyl)quinoline-2(1H)-thione. S-alkylation of this intermediate with (+)-biotinyl-iodoacetamidyl-3,6-dioxaoctanediamine led to biotinylated Arylquin 9 (Supplementary Note). Solvents were used from commercial vendors without further purification unless otherwise noted. Nuclear magnetic resonance spectra were determined on a Varian instrument (1H, 400MHz; 13C, 100Mz). High resolution electrospray ionization (ESI) mass spectra were recorded on a LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). The FT resolution was set at 100,000 (at 400 m/z). Samples were introduced through direct infusion using a syringe pump with a flow rate of 5 µL/min. MALDI mass spectra were obtained on a Bruker Utraflexstreme time-of-flight mass spectrometer (Billerica, MA), using DHB (2,5-dihydroxybenzoic acid) matrix. Purity of compounds was established by combustion analyses by Atlantic Microlabs, Inc., Norcross, GA. Compounds were chromatographed on preparative layer Merck silica gel F254 unless otherwise indicated.
Publication 2014
2,3-dihydroxybenzoic acid 2-aminobenzaldehyde acetyl chloride Alkylation Anabolism Arylquin 1 benzaldehyde benzyloxycarbonyl-valyl-alanyl-aspartic acid benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone Brefeldin A Caimans Ketones Lawesson's reagent Magnetic Resonance Imaging Mass Spectrometry MDM2 protein, human nutlin-3A quinoline Silica Gel Solvents Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Syringes Thiones
Mapping was completed using the apo form of the proteins submitted to the FTMap server, http://ftmap.bu.edu.21 (link) (The FTMap site was accessed between June and October of 2011, and will be maintained to remain functional in the future.) For nNOS, both the heme and the cofactor were modeled with the apo structure during mapping; all other ligands were removed for all other systems. The FTMap algorithm21 (link) uses 16 small molecules as probes (ethanol, isopropanol, isobutanol, acetone, acetaldehyde, dimethyl ether, cyclohexane, ethane, acetonitrile, urea, methylamine, phenol, benzaldehyde, benzene, acetamide, and N,N dimethylformamide) and consists of four steps as follows.

The rotational/translational space of each probe is systematically sampled on a grid around the fixed protein, consisting of 0.8 Å translations and of 500 rotations at each location. The energy function includes a stepwise approximation of the Van der Waals energy with attractive and repulsive contributions, and an electrostatics/solvation term based on the Poisson-Boltzmann continuum model with dielectric constants of ε=4 and ε=80 for the protein and the solvent respectively61 . The energy expression is written as the sum of correlations functions, and hence it can be very efficiently evaluated using fast Fourier transforms.21 (link) The 2000 best poses for each probe are retained for further processing.

The 2000 complexes are refined by off-grid energy minimization during which the protein atoms are held fixed while the atoms of the probe molecules are free to move. The energy function includes the bonded and van der Waals terms of the CHARMM potential62 and an electrostatics/solvation term based on the Analytic Continuum Electrostatic (ACE) model63 as implemented in CHARMM.

The minimized probe conformations are grouped into clusters using a simple greedy algorithm. The lowest energy structure is selected and the structures within 4 Å RMSD are joined in the first cluster. The members of this cluster are removed, and the next lowest energy structure is selected to start the second cluster. This step is repeated until the entire set is exhausted. Clusters with less than 10 members are excluded from consideration. The retained clusters are ranked on the basis of their Boltzman averaged energies. Six clusters with the lowest average free energies are retained for each probe.

In order to identify consensus clusters where a number of probe clusters overlap19 (link) the probe clusters are themselves clustered using 4 Å distance between cluster centers as the clustering radius. The consensus clusters are ranked on the basis of the number of probe clusters contained.21 (link) To determine if any of the consensus clusters includes the core moiety from the fragment screening, the core-bound protein is superimposed on the unbound protein results using PyMol to obtain appropriate positioning and orientation of the core fragment. If a consensus cluster has 5 or more atoms within 1.25 Å of the core, it is considered to coincide with the core moiety, and is identified as “core consensus cluster” in Table 2. Similarly, if a (non-core) consensus cluster has 5 or more atoms within 1.25 Å of any atom of an extended ligand, which is not part of the core, it is defined as “extension consensus cluster”.

Publication 2011
Acetaldehyde acetamide Acetone acetonitrile ARID1A protein, human benzaldehyde Benzene Cyclohexane dimethyl ether Dimethylformamide Disgust Electrostatics Ethane Ethanol Heme isobutyl alcohol Isopropyl Alcohol Ligands methylamine Molecular Probes NOS1 protein, human Phenol Protein Biosynthesis Proteins Radius Solvents Urea urinary gonadotropin fragment
Prior to conditioning, subjects were given food without Doxycycline for 2 days. During fear conditioning, subjects were placed in a shock chamber (30 × 35 × 32 cm) scented with 0.25% benzaldehyde in 70% ethanol for 500 s. (Ctx A). Foot shocks (0.75 mA, 2 s duration) were administered at the198 s, 278 s, 358 s, and 438 s time points. For reward conditioning, each subject was exposed to a single female mouse for 2 h in their home cage in a room separate from the housing room (Ctx B). Immediately after, in both fear and reward conditioning, subjects returned to a Dox (40 mg/kg) diet.
Publication 2014
benzaldehyde Diet Doxycycline Ethanol Fear Females Food Foot Mice, House Shock

Most recents protocols related to «Benzaldehyde»

The apricot kernel powder was added to the debitterizing wastewater at to the ratio (material to liquid) of 1:125 (g/mL), and then the pH was adjusted. Subsequently, the reaction was carried out at a certain temperature. The reaction product underwent steam distillation, followed by extraction with 80 mL of n-hexane and subsequent pressure concentration, resulting in the isolation of benzaldehyde.
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Publication 2024
Analysis of 2‐HPP and benzaldehyde was performed using a gas chromatograph (Agilent 6890) equipped with an FID detector, as described previously (Kordesedehi et al., 2023 (link)).
Publication 2024
Determination of benzaldehyde: An isocratic elution was applied for the separation of benzaldehyde with the mobile phase of methanol-H2O (35:65; v/v), a 1.0 mL/min flow rate, a 35 °C column temperature, a 250 nm detection wavelength and a 20 mL injection volume [14 (link)]. The standard curve was y = 1345.2442x − 0.6348 (R2 = 0.9998), and the content of benzaldehyde was calculated
Determination of amygdalin: We referred to Zhang’s method and made slight modifications. The HPLC working parameters were as follows: a sample injection volume of 20 mL, a 35 °C column temperature and a 1 mL/min flow rate, with the ratio of solvent A (methanol):solvent B (water) ¼ as 28:72 (v/v), and the wavelength was 214 nm [15 (link)]. The standard curve was y = 131.90764x − 3.04545 (R2 = 0.9984), and the content of amygdalin was calculated.
Determination of hydrocyanic acid: The pH of the solution was adjusted using acetic acid and sodium hydroxide, followed by the addition of chloramine T for the reaction. Subsequently, isonicotinic acid-barbituric acid was introduced to develop the color, and the absorbance was measured at a wavelength of 600 nm [16 (link)]. The standard curve was y = 0.85882x − 0.00781 (R2 = 0.9991), and the content of hydrocyanic acid was calculated.
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Publication 2024
Electrochemical measurements were carried out in a home-built flow cell with an electrode area of 10 × 2.7 cm2. To prepare the working electrode, β-Ni(OH)2 nanosheets (5.4 mg) were dispersed in 20 ml of a methanol solution containing 100 μl of ethylene glycol, with the resulting catalyst ink then sprayed onto carbon paper. The electrochemical test was performed on a high-power electrochemical workstation (CHI1130C, Shanghai Chenhua, China). Nickel foam (0.1 × 10 × 2.7 cm3) was used as the cathode. KOH (5.0 M) was used as the anodic and cathodic electrolytes, with a one-way flow rate of 1 ml min−1. A 9.3 M BA solution (15 ml of BA + 0.5 ml of cyclohexane) was injected into the organic flow chamber and cycled at a flow rate of 2 ml min−1. The flow rate was controlled by peristaltic pumps (T16, LHZW Technology, China). An 80% manual internal resistance compensation was performed for the flow cell benzaldehyde electrosynthesis test.
Publication 2024
In the Schlenk tube equipped with a stirring element, a ligand was placed (0.1 equiv.), and the tube was flushed three times with argon. Then, the solvent was added (1.25 mL). After 10 min, 1.1 M ZnEt2 in toluene (2 equiv., 0.945 mL) was added. After 30 min of stirring at room temperature, freshly distilled benzaldehyde (0.5 mmol, 51 μL) was added, and then the reaction was stirred at the indicated time until TLC showed the disappearance of the aldehyde. The reaction was diluted with diethyl ether and quenched with 1 M HCl. The organic layer was separated, and the aqueous layer was extracted twice with ether. The combined organic layers were then concentrated and purified by column chromatography (hexane-EtOAc 7:3) to give 1-phenyl-1-propanol as a colorless oil. The 1H-NMR (300 MHz, CDCl3) δ 7.43–7.23 (m, 5H, C6H5), 4.62 (t, J 6.6 Hz, 1H, PhCH(OH)Et), 1.90–1.70 (m, 2H, CH2), and 0.95 (t, J 7.4 Hz, 3H, CH3).
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Publication 2024

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Benzaldehyde is a clear, colorless liquid with a characteristic almond-like odor. It is a widely used organic compound that serves as a precursor and intermediate in the synthesis of various chemicals and pharmaceuticals.
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Hexanal is a chemical compound used as a reagent in various laboratory applications. It is a clear, colorless liquid with a pungent, grassy odor. Hexanal is commonly used as a standard or reference material in analytical procedures, particularly in the fields of chemistry, biochemistry, and food science.
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4-(dimethylamino) benzaldehyde is a chemical compound used in various laboratory applications. It is a crystalline solid with a yellow color. The compound is commonly used as a reagent in analytical and organic chemistry procedures.
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Acetic acid is a colorless, vinegar-like liquid chemical compound. It is a commonly used laboratory reagent with the molecular formula CH3COOH. Acetic acid serves as a solvent, a pH adjuster, and a reactant in various chemical processes.
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1-octen-3-ol is a colorless liquid compound used in laboratory settings. It serves as a chemical reagent and is a key ingredient in various analytical and research procedures. The core function of 1-octen-3-ol is to provide a specific chemical properties and reactions that are useful for scientific investigations and experiments.
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Benzyl alcohol is a colorless organic liquid that is commonly used as a solvent and preservative in various laboratory applications. It has a mild, aromatic odor and is miscible with water, alcohol, and many organic solvents. Benzyl alcohol is a versatile chemical that serves as a key component in numerous laboratory procedures and experiments.
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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The ALDEFLUOR assay kit is a fluorescent-based detection system designed to identify and isolate cells with high aldehyde dehydrogenase (ALDH) enzyme activity. ALDH is an enzyme that plays a role in stem cell function. The kit provides reagents and a protocol to measure ALDH activity in live cells using flow cytometry or fluorescence-activated cell sorting (FACS).

More about "Benzaldehyde"

Benzaldehyde is a versatile organic compound that has a wide range of applications in various industries.
It is a colorless, aromatic liquid with the chemical formula C6H5CHO, known for its distinctive almond-like odor.
Benzaldehyde is a naturally occurring compound found in many fruits and plants, and it serves as a precursor for numerous organic syntheses.
As an important industrial chemical, benzaldehyde is used in the production of pharmaceuticals, dyes, and other chemicals.
It is also commonly used as a flavoring agent in food and cosmetics.
Researchers studying benzaldehyde can utilize the AI-driven platform PubCompare.ai to optimize their research.
This innovative solution helps locate reliable protocols from literature, preprints, and patents, while providing insightful comparisons to identify the best products and procedures.
In addition to benzaldehyde, researchers may also be interested in studying related compounds such as hexanal, 4-(dimethylamino) benzaldehyde, acetic acid, 1-octen-3-ol, linalool, nonanal, benzyl alcohol, and methanol.
These compounds share structural similarities or functional properties with benzaldehyde and may be relevant in various research contexts.
The ALDEFLUOR assay kit is another tool that can be useful in benzaldehyde-related studies, as it is designed to detect and quantify aldehyde dehydrogenase (ALDH) activity, which is associated with various cellular processes.
By incorporating these insights and related terms, researchers can enhance the SEO optimization of their content, improving its visibility and accessibility to others in the field.
The resulting text should be informative, clear, and easy to read, providing a comprehensive overview of the topics surrounding benzaldehyde and its applications.