> Chemicals & Drugs > Organic Chemical > Berberine

Berberine

Berberine is a naturally occurring alkaloid with a wide range of potential health benefits.
It has been studied for its anti-inflammatory, antioxidant, and metabolic effects, making it a popular subject for research in areas like cardiovascular health, diabetes, and neurological disorders.
PubCompare.ai can help optimize your Berberine research by using AI-driven comparisons to identify the best protocols and products from literature, preprints, and patents, enhancing reproducibility and accuracy.
Easily locate the most relevant information and take your Berberine study to the next level with the power of PubCompare.ai.

Most cited protocols related to «Berberine»

Berberine Mitigates High-Fat Diet-Induced Diabetes

Cited 15 times

100 Wistar rats were randomly divided into 5
groups: control group (CON3), control plus STZ injection group (C-STZ), high-fat
diet group (HFD), high-fat diet plus STZ injection group (HFD-STZ), Berberine treated
high-fat diet plus STZ injection group (BER); control group and control plus
STZ injection group were fed with regular chow, and other three groups were
given high-fat diet for 4 weeks; the C-STZ, HFD-STZ groups and BER group were
injected IP with a low dose of STZ (according to the second section, choosing
the dose of the group with higher
successful rate: 30 mg/kg). After one week, FBG was measured in these three
groups, the rats with FBG < 7.8 mmol/L were injected with STZ again (30 mg/kg), while the control rats were
given vehicle citrate buffer (pH 4.4) in a dose volume of 0.25 mL/kg, IP,
respectively. The fasting blood glucose was measured every week. After 4 weeks of
STZ injection, the rats with the fasting blood glucose of ≥7.8 mmol/L twice or with nonfasting blood glucose of ≥11.1 mmol/L were considered diabetic. Berberine (100 mg/kg body weight) was
administered orally as suspension by mixing with vehicle 1% Na-CMC at a dose
volume of 0.5 mL/kg body weight of rats in treatment group for another 4 weeks.
The body weight and food intake of the animals were also measured. The rats were
allowed to continue to feed on their respective diets until the end of the
study. At the end of the study, IPGTT and ITT were conducted in the five groups;
fasting plasma was collected for further measurement of insulin, TG, TC, and
glucose. The insulin sensitivity index (ISI) was calculated according to the fasting insulin and glucose
concentration.

Zhang M., Lv X.Y., Li J., Xu Z.G, & Chen L. (2009). The Characterization of High-Fat Diet and Multiple Low-Dose Streptozotocin Induced Type 2 Diabetes Rat Model. Experimental Diabetes Research, 2008, 704045.

Full text: Click here

Publication 2008

Berberine Blood Glucose Body Weight Buffers Citrates Diet Diet, High-Fat Feeds, Animal Insulin Insulin Sensitivity Plasma Rats, Wistar Rattus norvegicus Therapy, Diet

Singlet Oxygen Production Assay

Cited 6 times

An ethanolic DMA solution was prepared (0.35 mM) and transferred (190 µL per well) into of a 96-well plate. A DMSO solution (10 µL, 1 mg mL⁻¹) of each extract was added to the respective wells. The absorption of the extract alone (50 µM, ethanol) was measured as well as the suppression of the DMA absorbance in the presence of l-ascorbic acid (5 mM, pH 7.0–7.4). An additional control experiment (extract and l-ascorbic acid) was performed. DMSO (5% in ethanol) was used as solvent control. Berberine (50 µg mL⁻¹, 149 µM) was used as positive control. Excitation was performed with the 96 LED setup, whereby four irradiation steps (468 nm, 5 min each, 6.2 J cm⁻² each) were chosen. The measurement of the absorption was performed with a microplate reader (Tecan, Spark M10) and done in triplicate. The singlet oxygen production after 20 min (24.8 J cm⁻²) was calculated relative to berberine using formula (eqn (2)). A detailed discussion of this formula can be found in the ESI.‡

Siewert B., Vrabl P., Hammerle F., Bingger I, & Stuppner H. (2019). A convenient workflow to spot photosensitizers revealed photo-activity in basidiomycetes. RSC Advances, 9(8), 4545-4552.

Full text: Click here

Publication 2019

Ascorbic Acid Berberine Ethanol Radiotherapy Singlet Oxygen Solvents Sulfoxide, Dimethyl

Extracellular Recordings of Drosophila Taste Sensilla

Cited 6 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2011

Action Potentials Apathy Berberine Caffeine Chest Diptera Drosophila Head Ringer's Solution Sensilla

Erythrocyte Cytotoxicity Evaluation Protocol

Cited 5 times

Cytotoxicity was determined in a suspension of human erythrocytes by the hemolysis test. Serum was separated and EDTA (1.5 mg/mL blood) was added to wash out the erythrocytes, which mix and separate at 1,000 rpm (5 min/37 °C). The erythrocytes were centrifuged three times in pH 7.4 (10 mM PBS) phosphate buffer solution removing the supernatants. The erythrocytes obtained were used to prepare the 5% v/v red blood cell suspension in PBS). For the evaluation of Am and berberine cytotoxicity in erythrocytes, the prepared suspension was incubated with different concentrations of the extract, berberine and controls (50 to 1,000 μg/mL) for 30 min (37 °C), these were classified as treatments (Tr). The negative control was untreated erythrocytes (C-), positive control was distilled water to produce osmotic hemolysis (C+) (18 ). After incubation, treatments were centrifuged for 4 min at 13,000 rpm (4 °C), 200 μL were taken and placed in a 96-well microplate. Hemolysis was determined spectrophotometrically at 540 nm. The readings were recorded as absorbance (Abs) for each treatment minus the absorbance presented by the vehicle (AbsTr). The percentage of hemolysis was calculated with the formula:

% Hemolysis = \frac{(AbsTr) - (AbsC -)}{(AbsC +) - (AbsC -)} \times 100

ELIZONDO-LUÉVANO J.H., CASTRO-RÍOS R., VICENTE B., FERNÁNDEZ-SOTO P., LÓPEZ-ABAN J., MURO A, & CHÁVEZ-MONTES A. (2021). In Vitro Antischistosomal Activity of the Argemone mexicana Methanolic Extract and Its Main Component Berberine. Iranian Journal of Parasitology, 16(1), 91-100.

Publication 2021

Berberine BLOOD Buffers Cytotoxin Edetic Acid Erythrocytes Hemolysis Homo sapiens Osmosis Phosphates Serum

Comprehensive Root Tissue Analysis

Cited 5 times

Modified pseudo-Schiff propidium iodide (mPS-PI) staining was performed as described for floral stalks in Truernit et al. (2008 (link)) on root tips of plants 8 DAG. The staining with Schiff reagent and PI was carried out using vacuum. The samples were examined with either a 25x oil objective with a numeric aperture (NA) of 0.8 using a Zeiss laser scanning microscope (LSM) 510 Meta or a 40x water objective with a NA of 1.20 using a Zeiss LSM 780. PI was excited with a 561 nm Argon laser with emission detection at 566–718 nm.
For cross sections of the root hair zone, roots were embedded in melted 5% agarose and sectioned manually with a sharp razor blade. Endodermis staining with berberine hemisulfate was carried out as described in Lux et al. (2005 (link)). The samples were examined with a 40x water objective with a NA of 1.20 using a Zeiss LSM 780. Green fluorescence was excited with a 488 nm Argon laser with emission detection at 490–544 nm. Transmitted light pictures were taken with a transmitted light detector (T-PMT).
EdU staining was performed with the Click-iT EdU Imaging Kit (Invitrogen) and the fluorophor Alexa568 as described in the manufacturer's manual with the following modifications: root tips of plants 8 DAG were covered with 10 μM EdU in dH₂O and placed in the phytochamber for the respective incubation time. Root tips were fixed for 1 h under vacuum and permeabilized for 1 h at room temperature. The Click-iT reaction was carried out for 1 h under vacuum in darkness. DNA-counterstaining was performed with 1 μg/ml DAPI in PBS for 1 h in darkness under vacuum. The samples were cleared for around 14 days at 4°C in clearing solution described in Warner et al. (2014 (link)). The roots were examined with a 40x water objective with a NA of 1.20 using the Zeiss LSM 780. DAPI was excited with a 405 nm Diode with emission detection at 410–560 nm, Alexa568 was excited with a 514 nm Argon laser and emission was detected at 545–697 nm in a separate track. The pinhole was set to 2,05 Airy units. Pictures were taken with the tile scan function with 10% overlap, a threshold of 0.70 and automatically stitched by the microscope software.
RNA in situ hybridizations were examined with a plan-neofluar 20x objective with a NA of 0.50 or a plan-neofluar 40x objective with a NA of 0.75 using a Zeiss Axioskop light microscope.

Kirschner G.K., Stahl Y., Von Korff M, & Simon R. (2017). Unique and Conserved Features of the Barley Root Meristem. Frontiers in Plant Science, 8, 1240.

Full text: Click here

Publication 2017

A-A-1 antibiotic Argon Ion Lasers Berberine DAPI Darkness Endoderm Fluorescence GART protein, human Hair In Situ Hybridization Laser Scanning Microscopy Light Light Microscopy Microscopy Plant Roots Plants Propidium Iodide Radionuclide Imaging Root Tip Schiff's reagent Sepharose Stalking Vacuum

Most recents protocols related to «Berberine»

HPLC for Quality Control of Herbal Extract

HPLC was used for quality control of the total extract. The analysis was performed in an Agilent 1260 system, including G1315D diode array detector and ChemStation chromatography workstation. Chromatographic separation was performed with an Agilent Hypersil BDS C₁₈ column (250 mm × 4.6 mm, 5 μm) and monitored at 280 nm at 30 °C. The mobile phase was a mixture of water containing 0.3% phosphoric acid (triethylamine controlled pH 4) (A) and acetonitrile (B), and the elution method was as follows: 0–18 min (30–40% B), 18–25 min (40–57% B) with a flow rate of 1 ml/min. The injection volume was 10 μl (0.5 g/ml). Berberine hydrochloride (RT 8.18 min; purity >98%; lot: 165230–201203) and 6-gingerol (RT 22.65 min; purity >98%; lot: 0713–9906) were purchased from National Institutes for Food and Drug Control, China, and used as chemical markers.

Sun Y., Yu C.L., Yan Y.L., Zhang F.L., Chen J., Hu Z.Y., He J., Meng X.Y, & Wu Q.F. (2023). Inhibitory Effects and Related Molecular Mechanisms of Huanglian-Ganjiang Combination Against H1N1 Influenza Virus. Revista Brasileira De Farmacognosia, 33(3), 514-522.

Publication 2023

acetonitrile Berberine Chromatography Food gingerol High-Performance Liquid Chromatographies phosphoric acid triethylamine

Chitosan-based Nanoparticles for Drug Delivery

Chitosan-based nanoparticles including blank CS NPs, SF-loaded chitosan nanoparticles (SF-CS NPs), and Tween 80-coated SF-loaded CS-NPs (T- SF-CS NPs) were prepared using essentially a TPP ionic gelation method with some modification [25 (link)]. In brief, low molecular weight CS was dissolved in an acetic acid solution with magnetic stirring overnight. After pH adjustment to 4.7-4.8 with 20% NaOH, the CS solution was filtered using a 0.45 μm nylon syringe filter. A cold-filtered TPP solution (3 mL) was added to the CS solution (10 mL) in a water bath at 60°C under magnetic stirring for about 10 min and the formed blank SF-CS NPs were separated. To adjust the physical properties of plain CS-NPs, particularly, the particle size and size distribution, a series of preliminary trials based on the preparation of NPs by changing the experimental variables one at a time while keeping other variables constant was undertaken. The variables included the concentration of CS and TPP solutions (0.5 mg/mL vs. 2 mg/mL), the temperature of the CS solution during TPP addition (25°C vs. 60°C) as well and the method of separation of the formed CS NPs (low-speed centrifugation at 3000 rpm for 10 min at ambient temperature vs probe sonication for 10 and 20 min).
SF-CS-NPs were prepared by dissolving SF (2 mg/mL) in the CS solution as reported earlier for berberine-loaded CS NPs [26 (link)], and the procedure was completed as described above. For the preparation of T-SF-CS-NPs, freshly prepared SF-CS NPs were resuspended in 1% Tween 80 solution and sonicated for 20 min in a water bath sonicator [27 (link)].

Sheweita S.A., Alian D.M., Haroun M., Nounou M.I., Patel A, & El-Khordagui L. (2023). Chitosan Nanoparticles Alleviated the Adverse Effects of Sildenafil on the Oxidative Stress Markers and Antioxidant Enzyme Activities in Rats. Oxidative Medicine and Cellular Longevity, 2023, 9944985.

Full text: Click here

Publication 2023

Acetic Acid Anabolism Bath Berberine Centrifugation Chitosan Cold Temperature Ions Nylons Physical Processes Syringes Tween 80

Standardized Extraction of YQF Components

YQF consists of Panax ginseng C.A.Mey (Renshen), Coptis chinensis Franch (Huanglian), and Ligusticum chuanxiong S.H.Qiu, Y.Q.Zeng, K.Y.Pan, Y.C.Tang and J.M.Xu (Chuanxiong). These herbs were purchased from Hebei Baicao Kangshen Pharmaceutical Co., Ltd., (Hebei, China). Donepezil hydrochloride (production lot No. 2104113; Shenzhen Anlixin, Shenzhen, China) was purchased from Eisai (Tokyo, Japan). Ginsenosides Rg1 reference substance, purity 99.70% (A0237); ginsenosides Re reference substance, purity 99.52% (A0244); ginsenosides Rd reference substance, purity 98.55% (A0245); ginsenosides Rb1 reference substance, purity 98.58% (A0234); ferulic acid reference substance, purity 99.32% (A0050); provided by Chengdu Desite Biotechnology Co., Ltd., Chengdu, China. Ginsenosides Rh2 reference substance, purity 98% (B21729); Ginsenosides Rg2 reference substance, purity 98% (B21727); epiberberine reference substance, purity 98% (B20108); coptisine chloride reference substance, purity 98% (B21438); palmatine reference substance, purity 98% (B21646);berberine reference substance, purity 98% (B21379); and provided by Shanghai Yuanye Biotechnology Co., Ltd., Shanghai, China.

Wei W., Pei H., Ma L.N., Zheng R., Huang Q.Y., Chang S.R., Cao Y, & Li H. (2023). Comparison of Yizhiqingxin formula extraction methods and their pharmacodynamic differences. Frontiers in Neuroscience, 17, 1097859.

Full text: Click here

Publication 2023

Berberine Coptis chinensis coptisine chloride Donepezil Hydrochloride epiberberine ferulic acid ginsenoside Rb1 ginsenoside Rd ginsenoside Re ginsenoside Rg1 ginsenoside Rg2 ginsenoside Rh2 huanglian Lovage, Alpine palmatine Panax ginseng Pharmaceutical Preparations

Preparation and Characterization of ZYP Decoction

ZYP decoction was prepared with Huanglian (Coptis chinensis Franch., 5g, Cat: 21080108) and Wuzhuyu (Tetradium ruticarpum (A. Jussieu) T. G. Hartley, 5g, Cat: 21110103). The herb mixtures were boiled two times in water. For the first time, the Huanglian and Wuzhuyu tablets were mixed to 40 g at a ratio of 1:1 and heated with 500 mL water at 100 °C for 30 min, and then filtrated to gather the filtrate. Herb broth was collected at the second time by adding 250 mL water to the filtered crude herbs, boiling for another 30 minutes and filtering. After that, a freeze-drying step followed the concentration of both herb broth. According to our previous high performance liquid chromatography (HPLC) results, ZYP contained 36.8 mg/g of berberine, 14.9 mg/g of coptisine, 0.78 mg/g of evodiamine, and 0.33 mg/g of rutecarpine.17 (link)

Pan Y., Feng X., Song W., Zhou X., Zhou Z., Chen G., Shen T, & Zhang X. (2023). Effects and Potential Mechanism of Zhuyu Pill Against Atherosclerosis: Network Pharmacology and Experimental Validation. Drug Design, Development and Therapy, 17, 597-612.

Publication 2023

Berberine Coptis chinensis coptisine Euodia ruticarpa evodiamine High-Performance Liquid Chromatographies huanglian rutecarpine

Identification of Targets for Bioactive Components

The bioactive components of ZYP were acquired from our previous study. They are berberine, coptisine, evodiamine, and rutaecarpine.17 (link) Targets of these four chemical components were obtained from the TCMSP database (

https://old.tcmsp-e.com/tcmsp.php

), the SwissTargetPrediction database (

http://www.swisstargetprediction.ch/

), and the STITCH database (

http://stitch.embl.de/

). Since the targets’ names collected from the TCMSP database were not standardized, they were transformed using the UniProt database (

https://www.uniprot.org

). Targets collected from different databases were merged and the duplicates were removed.

Publication 2023

Berberine coptisine evodiamine rutecarpine

Top products related to «Berberine»

Berberine by Merck Group

Sourced in United States, Germany, Sao Tome and Principe, China

Berberine is a natural compound extracted from various plant species. It is a highly purified, powdered substance used in laboratory research and analysis. Berberine has chemical and biological properties that make it a useful tool for scientific investigation, but its specific applications and intended uses are not provided here.

Berberine chloride by Merck Group

Sourced in United States, United Kingdom

Berberine chloride is a natural compound that can be used as a laboratory reagent. It is a crystalline solid that is soluble in water and organic solvents. Berberine chloride is commonly used in research applications, but its detailed use and function should not be interpreted or extrapolated upon in this response.

Dimethyl sulfoxide (dmso) by Merck Group

Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh

DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal

Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Acetonitrile by Merck Group

Sourced in Germany, United States, Italy, India, China, United Kingdom, France, Poland, Spain, Switzerland, Australia, Canada, Brazil, Sao Tome and Principe, Ireland, Belgium, Macao, Japan, Singapore, Mexico, Austria, Czechia, Bulgaria, Hungary, Egypt, Denmark, Chile, Malaysia, Israel, Croatia, Portugal, New Zealand, Romania, Norway, Sweden, Indonesia

Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.

Formic acid by Merck Group

Sourced in Germany, United States, Italy, United Kingdom, France, Spain, China, Poland, India, Switzerland, Sao Tome and Principe, Belgium, Australia, Canada, Ireland, Macao, Hungary, Czechia, Netherlands, Portugal, Brazil, Singapore, Austria, Mexico, Chile, Sweden, Bulgaria, Denmark, Malaysia, Norway, New Zealand, Japan, Romania, Finland, Indonesia

Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.

Methanol by Merck Group

Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan

Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.

Berberine hydrochloride by Merck Group

Sourced in United States, United Kingdom, Germany

Berberine hydrochloride is a chemical compound used in laboratory research. It is a yellow crystalline powder that is soluble in water. Berberine hydrochloride has applications in various fields of study, including biochemistry and molecular biology.

Acetonitrile by Thermo Fisher Scientific

Sourced in United States, United Kingdom, China, Belgium, Germany, Canada, Portugal, France, Australia, Spain, Switzerland, India, Ireland, New Zealand, Sweden, Italy, Japan, Mexico, Denmark

Acetonitrile is a highly polar, aprotic organic solvent commonly used in analytical and synthetic chemistry applications. It has a low boiling point and is miscible with water and many organic solvents. Acetonitrile is a versatile solvent that can be utilized in various laboratory procedures, such as HPLC, GC, and extraction processes.

Quercetin by Merck Group

Sourced in United States, Germany, Italy, India, Spain, United Kingdom, France, Poland, China, Sao Tome and Principe, Australia, Brazil, Macao, Switzerland, Canada, Chile, Japan, Singapore, Ireland, Mexico, Portugal, Sweden, Malaysia, Hungary

Quercetin is a natural compound found in various plants, including fruits and vegetables. It is a type of flavonoid with antioxidant properties. Quercetin is often used as a reference standard in analytical procedures and research applications.

More about "Berberine"

Berberine is a naturally occurring alkaloid that has garnered significant interest for its potential health benefits.
This versatile compound has been extensively studied for its anti-inflammatory, antioxidant, and metabolic effects, making it a popular subject for research in various fields, including cardiovascular health, diabetes, and neurological disorders.
One of the key advantages of Berberine is its ability to modulate a wide range of biological processes.
For instance, it has been shown to exhibit anti-inflammatory properties, which may be beneficial in addressing conditions characterized by chronic inflammation, such as cardiovascular disease, metabolic disorders, and neurological conditions.
Similarly, Berberine's antioxidant properties have been explored, as it can help mitigate the damaging effects of oxidative stress, a key contributor to various health problems.
Researchers have also investigated Berberine's potential to positively impact metabolic processes, suggesting its possible applications in the management of conditions like diabetes and obesity.
Beyond its therapeutic potential, Berberine has also been studied in the context of other compounds, such as Berberine chloride, DMSO, FBS, Acetonitrile, Formic acid, Methanol, and Berberine hydrochloride.
These substances may play a role in the extraction, purification, or formulation of Berberine-based products, contributing to the overall understanding and utilization of this remarkable natural compound.
To optimize your Berberine research, PubCompare.ai offers a powerful tool that leverages AI-driven comparisons to identify the best protocols and products from literature, preprints, and patents.
By utilizing this platform, you can enhance the reproducibility and accuracy of your studies, while also easily locating the most relevant information to take your Berberine research to the next level.
Experience the power of PubCompare.ai and unlock the full potential of this versatile natural compound.