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Betadine

Betadine is a topical antiseptic solution containing povidone-iodine, commonly used to cleanse and disinfect skin before medical procedures.
It has broad-spectrum antimicrobial properties and is effective against a variety of bacteria, viruses, fungi, and protozoa.
Betadine is often utilized to prevent surgical site infections, treat minor cuts and abrasions, and prepare the skin for intravenous access.
Researchers can explore the efficacy and applications of Betadine through PubCompare.ai's AI-driven platform, which facilitates the identification of optimal research protocols and products.
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Most cited protocols related to «Betadine»

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Publication 2012
Anesthesia Animals Asepsis Betadine Clostridium Collagenase collagenase 1 Cranium Dura Mater Gender Institutional Animal Care and Use Committees Isoflurane Loma Matrix Metalloproteinase 13 Needles Operative Surgical Procedures Oxygen Rats, Sprague-Dawley Reading Frames Scalp Sutures Syringes Trephining
Approval was obtained from the University Committee on Animal Research. Six-to-eight-week-old female C57BL/6 mice (Jackson Laboratories, Bar Harbor, ME) were anesthetized by intraperitoneal injection of 4 mg/kg xylazine and 60 mg/kg ketamine. The right plantar paw and medial hind limb were shaved and prepared with alcohol and betadine. Under magnification, a longitudinal incision was made starting from the base of the digits of the plantar hind foot and extending proximally to the mid-postero-medial calf. The distal FDL tendon was exposed and two horizontal 8-0 nylon sutures in a modified Kessler pattern were placed in the intact tendon. The tendon was then transected between the sutures and repaired by approximating the transected ends using the suture. The tendon was also transected proximally along the tibia at the myotendinous junction to protect the repair. The skin was closed with running 4-0 nylon sutures. The mice were returned to their cages and allowed free active motion and weight bearing following recovery from anesthesia. On days 3, 7, 10, 14, 21, 28, and 35 post-repair, animals were sacrificed and the tendons were harvested for histological analysis (N = 5 repairs per time point and 5 age-matched contralateral sham specimens per time point), in situ hybridization, and RNA extraction (N = 5 repairs per time point). Specimens for biomechanical testing were harvested at 0, 14, 21, 28, 42, and 63 days post-repair (N = 8–10 repairs per time point with age matched contralateral sham specimens harvested from each mouse at each time point).
Sham surgeries were performed on control groups. Identical anesthesia and exposure were performed. When the distal FDL tendon was isolated, two horizontal 8-0 sutures in a modified Kessler pattern were sutured through the tendon. This suture was then removed without transecting the tendon. However, the proximal FDL tendon was released at the myotendinous junction as in the repair group. The skin was closed with running 4-0 nylon sutures.
Publication 2009
Anesthesia Animals Betadine Ethanol Females Hindlimb Injections, Intraperitoneal In Situ Hybridization Ketamine Mice, House Mice, Inbred C57BL Myotendinous Junction Nylons Operative Surgical Procedures Skin Sutures Tendons Tibia Toes Wound Healing Xylazine
A nonlethal model of segmental (70%) hepatic warm ischemia was used. The I/R protocol was initiated with the abdominal wall being clipped of hair and cleansed with betadine. Under sodium pentobarbital (40 mg/kg, i.p.) and methoxyflurane (inhalation) anesthesia, a midline laparotomy was performed. With the use of an operating microscope, the liver hilum was dissected free of surrounding tissue. All structures in the portal triad (hepatic artery, portal vein, bile duct) to the left and median liver lobes were occluded with a microvascular clamp (Fine Science Tools) for 60 min; reperfusion was initiated by removal of the clamp. This method of segmental hepatic ischemia prevents mesenteric venous congestion by permitting portal decompression through the right and caudate lobes. The abdomen was covered with a sterile plastic wrap to minimize evaporative loss. Throughout the ischemic interval, evidence of ischemia was confirmed by visualizing the pale blanching of the ischemic lobes. The clamp was removed and gross evidence of reperfusion that was based on immediate color change was assured before closing the abdomen with continuous 4–0 polypropelene suture. The absence of ischemic color changes or the lack of response to reperfusion was a criterion for immediate sacrifice and exclusion from further analysis. Temperature was monitored by rectal temperature probe and was maintained at 37°C by means of a warming pad and heat lamp. At the end of the observation period following reperfusion, the mice were anesthetized with inhaled methoxyflurane and were killed by exsanguination.
Publication 2005
Abdominal Cavity Anesthesia, Inhalation Betadine Decompression Duct, Bile Exsanguination Hair Hepatic Artery Ischemia Laparotomy Liver Mesentery Methoxyflurane Microscopy Mus Pentobarbital Sodium Rectum Reperfusion Sterility, Reproductive Sutures Tissues Triad resin Veins, Portal Wall, Abdominal Warm Ischemia
When mice were 2 to 3 months of age they were anesthetized with 5% isoflurane and placed in a stereotaxic frame with rounded Kopf head holders (David Kopf Instruments, Tujunga, CA). Pointed ear bars were not used. Temperature was controlled at 37°C using a feedback temperature controller (Cell Microcontrols, Norfolk, VA). Isoflurane was delivered by nose cone at 2% in air. The heads were shaved and prepped with Betadine. A midline skin incision was made and the skull was exposed. No craniotomy was performed.
A rubber tip (Precision Associates, Inc., Minneapolis, MN) was mounted on an electromagnetic stereotaxic impact device in place of the metal tip used in previous controlled cortical impact studies (21 (link)). The rubber tip was 9 mm in diameter and the rubber had a spring constant of 3.01 N/mm. The tip was fully extended and lowered at a 20° angle until the vertex touched the skull at 1.8 mm caudal to bregma and 3.0 mm left of midline. This was confirmed with a hand lens in all cases. The tip was then retracted automatically. The stereotaxic device was moved down by 3.3 mm and the electromagnetic device was triggered, driving the tip 3.3 mm into the exposed skull at 5.0 m/s with a dwell time of 100 milliseconds. The deformation of the rubber tip spread the impact force over the skull. There were <3% skull fractures and no immediate fatalities after these injuries. Mice with skull fractures were killed and were not used in any experiments. After impact, the skin was sutured and the mice were allowed to recover from anesthesia on a warming pad and then returned to their home cages. After 24 ± 1 hour, a second identical closed-skull TBI procedure was performed. For sham injuries, the same procedure was performed except that the impact device was discharged in the air; the handling of the mice and duration of anesthesia were the same for TBI and sham procedures.
Impacts and sham procedures were performed by 2 independent investigators (Y.S. and R.E.B.) without direct contact during the procedures. Behavioral performance, silver staining, and Iba1 immunohistochemical appearance were essentially indistinguishable for injuries performed by the 2 investigators. The mice used for behavioral assessments were not used for histological analyses. Nonetheless, after completion of behavioral testing these mice were killed and their brains were examined for hemorrhage. Mice with hemorrhages were excluded from the behavioral analyses.
Publication 2011
Anesthesia Betadine Brain Cells Cortex, Cerebral Craniotomy Cranium Electromagnetics Head Hemorrhage Injuries Isoflurane Lens, Crystalline Medical Devices Metals Mice, Laboratory Nose Reading Frames Retinal Cone Rubber Skin Skull Fractures
Seven adult barn owls were used in this study. Bird care and surgical and Materials and Methods were approved by the Stanford University Institutional Animal Care and Use Committee and were in accordance with the National Institutes of Health and the Society for Neuroscience guidelines for the care and use of laboratory animals.
Owls were prepared for multiple electrophysiological experiments. Before the first experiment, an owl was anesthetized with isofluorane (1%) and a mixture of nitrous oxide and oxygen (45:55), and a head bolt was mounted at the rear of the skull. Plastic cylinders that permitted access to the brain were implanted over the optic tectum bilaterally. Polysporin antibiotic ointment was applied to the exposed brain surface, and the recording chambers were sealed. All wounds were cleaned with betadine and infused with a local anesthetic. After recovering from surgery, the owl was returned to its flight room.
On the day of an experiment, the owl was anesthetized with isofluorane (1%) and a mixture of nitrous oxide and oxygen (45:55) and was placed in a restraining tube in a prone position within a sound-attenuating booth. The head was secured to a stereotaxic device, and the visual axes were aligned relative to a calibrated tangent screen (the eyes of the owl are essentially stationary in the head). Owls were maintained on just the mixture of nitrous oxide and oxygen from the time when they were secured in the booth through the end of the experiment.
Epoxy-coated tungsten microelectrodes (4-6 MΩ at 1kHz) were used to record extracellularly from single and multiple units (sites) in the intermediate and deep layers (layers 11-15) of the OT. These layers were identified by distinctive neuronal responses that have been associated reliably with these layers based on lesion-reconstruction experiments (DeBello and Knudsen, 2004 (link)). Visual and auditory stimuli were presented for 250 ms. Spikes were recorded from 400-1000 ms before stimulus onset until 500-1250 ms after stimulus onset. Inter-stimulus intervals (ISIs) ranged from 2.5 to 5.0 s, and the number of trials repeated at a stimulus location ranged from 10-25. Spike times were stored using Tucker-Davis hardware (RA-16) controlled by customized MATLAB (The Mathworks, Inc., Natick, MA) software.
Publication 2010
Adult Animals, Laboratory Antibiotics Auditory Perception Aves Barn Owls Betadine Brain Cranium Epistropheus Epoxy Resins Eye Head Institutional Animal Care and Use Committees Local Anesthesia Medical Devices Microelectrodes Neurons Operative Surgical Procedures Oxide, Nitrous Oxygen polysporin ointment Reconstructive Surgical Procedures Sound Strigiformes Tectum, Optic Tungsten Wounds

Most recents protocols related to «Betadine»

For all surgeries, mice were anesthetized with 1–2% isoflurane, and placed in a stereotaxic apparatus (Kopf Instruments) on a heating pad (Harvard Apparatus). Fur was removed from the scalp, the incision site was cleaned with betadine and a midline incision was made. Sterile surgical techniques were used, and mice were injected with sustained-release buprenorphine for post-operative recovery. Mice were allowed to recover for at least two weeks after surgery before behavioural experiments.
For intracranial optogenetic experiments, virus was injected using a 33-gauge beveled needle and a 10-µl Nano-fil syringe (World Precision Instruments), controlled by an injection pump (Harvard Apparatus). Five hundred nanolitres of AAVdj-hSyn::iC++-eYFP or AAVdj-hSyn::eYFP (5×1011 vg ml−1) was injected at 150 nl per min and the syringe was left in place for at least 10 min before removal. The following coordinates were used (relative to Bregma): posterior insula (−0.58 (anterior–posterior (AP)), ±4.2 (medial–lateral (ML)), −3.85 (dorsal–ventral (DV)); mPFC (1.8 (AP), ±0.35 (ML), −2.9 (DV)). Optical fibres (0.39 NA, 200 µm; Thorlabs) were implanted 200 µm above virus injection coordinates. Fibres were secured to the cranium using Metabond (Parkell). Mice were allowed to recover for at least two weeks before behavioural testing.
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Publication 2023
Betadine Buprenorphine Cranium Fibrosis Insula of Reil Isoflurane Mice, House Needles Operative Surgical Procedures Optogenetics Scalp Sterility, Reproductive Syringes Virus
Bovine Collagen type I, 8 wt.% aqueous solution (Coll, Collado s.r.o., Brno, Czech Republic), chitosan from shrimp shells, 70% DDA, low viscosity (Chit, Sigma-Aldrich, Darmstadt, Germany), calcium salt of oxidized cellulose–degree of oxidation 16–24% and Mn = 350 kg/mol (CaOC, Synthesia, Pardubice, Czech Republic), acetic acid (99%, Penta s.r.o, Chrudim, Czech Republic), poly(ε-caprolactone) (PCL, 80 kg/mol), gelatin (Gel, Type B, Bioreagent, powder from bovine skin), N-(3-Dimethylaminopropyl)-N´-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), 98% dopamine hydrochloride, tris (hydroxymethyl) aminomethane hydrochloride, ethanol p.a. 99.8%, sodium phosphate dibasic for molecular biology (≥ 98,5%), sodium chloride, calcium chloride, sodium phosphate dibasic dodecahydrate (Na2HPO4 ·12H2O), potassium dihydrogen phosphate (KH2PO4), potassium chloride (KCl), collagenase from Clostridium histolyticum, lysozyme human, the murine fibroblast cell lines 3T3-A31, Dulbecco's modified eagle medium DMEM (D6429), fetal bovine serum FBS (F7524), 2′,7′-bis (2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM), propidium iodide (P4864), (all from Sigma Aldrich, Darmstadt, Germany), penicillin/streptomycin (15140–122) and DiOC6(3) (D273), (Life Technologies, Eugene, OR, USA), octenidine solution (Octenisept®, Schülke, Germany), Butomidor® inj. (butorphanol tartrate, Vétoquinol, Czech republic), Domitor®, Medetomidine, Orion corporation, Finland) Propofol® (Propofolum 1%, Fresenius Kabi Deutschland, Bad Homburg, Germany), Metacam® (meloxicam, Boehringer Ingelheim Vetmedica, Ingelheim/Rhein, Germany), Enroxil® (Enrofloxacin, Krka, Novo mesto, Slovenia) Betadine®, (2.5% solution of povidone iodine, EGIS Pharmaceuticals PLC, Budapest, Hungary) were used as received without further purification.
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Publication 2023
2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 3,3'-dihexaoxycarbocyanine iodide Acetic Acid Betadine Bos taurus Butorphanol Tartrate Calcium, Dietary Calcium chloride caprolactone carboxyfluorescein Cell Lines Chitosan chloropentaammineosmium(III) chloride Collagenase, Clostridium histolyticum Collagen Type I Eagle Enrofloxacin Esters Ethanol Fibroblasts Gelatins Homo sapiens Hydrochloride, Dopamine Medetomidine Meloxicam methylamine hydrochloride Muramidase Mus N-hydroxysuccinimide octenidine Octenisept Oxidized Cellulose Penicillins Pharmaceutical Preparations Poly A potassium phosphate, monobasic Povidone Iodine Powder Propidium Iodide Propofol rhein Skin Sodium Chloride sodium phosphate Streptomycin Tetranitrate, Pentaerythritol Tromethamine Viscosity
The first suture is a 4-0 poliglecaprone (Monocryl) between point A and the 12 o’clock point of the NAC. The second suture is a 3-0 polydioxanone (PDS II) between points B and C and the 6 o’clock point of the NAC (Fig. 4D). Superior traction is applied to the ends of the second suture.3 (link),4 (link) This elevates the inferior vertical limb, which is then closed with deep interrupted 3-0 polydioxanone (PDS II) sutures.
The peri-areolar closure is completed with 4-0 poliglecaprone (Monocryl) deep interrupted sutures at the 3 and 9 o’clock positions, followed by four more sutures, halfway between these points. A 3-0 white polyester suture soaked in Betadine is then placed as a buried purse-string suture around the areola, which is then tightened around the areola marker.37 ,38 Placement of the purse-string suture also helps define the length of the vertical scar.
The patients are placed in a seated position. If necessary, tailor tacking is performed on the vertical inferior ellipse and any planned horizontal closure in the IMF. Usually, as the vertical incision is closed, the IMF establishes its own level, and a vertical incision continues onto the abdominal wall (Fig. 4E). Excess subcutaneous fat at the inferior end of the vertical incision can be reduced using liposuction or direct excision. If an IMF horizontal excision is performed, it is closed with deep 3-0 polydioxanone. The primary author prefers to evaluate the breast shape postoperatively performing a delayed horizontal excision after 6 months.
The final closure of the vertical and horizontal incision is completed with deep interrupted and subcuticular 4-0 poliglecaprone. The final periareolar closure is a subcuticular 4-0 poliglecaprone. The closure of the vertical incision is initiated 1 cm below the 6 o’clock position of the areola, to prevent areola creep3 (link) and distortion of the areola. Half-inch Steri strips are applied.
Xeroform gauze is placed on the areola with the nipple exposed, followed by gauze secured with Tegaderm. A soft postsurgical bra (Design Veronique) is applied but is optional.
Publication 2023
Areola Betadine Breast Cicatrix Monocryl Nipples Patients Polydioxanone Polyesters Silene Sitting Subcutaneous Fat Suction Lipectomy Sutures Traction Wall, Abdominal Xeroform
As previously described, the caecal ligation and puncture (CLP) method was carried out in mice.33 (link) First, after anaesthetization and disinfection with betadine solution, we performed laparotomy in the abdomen to expose the caecum and the adjoining intestine of mice. Then, a 6.0 thread suture was used to tightly ligate the intestinal canal under the ileocaecal section, puncture the canal wall with a 19-gauge needle, and gently squeeze a small number of intestinal contents into the abdominal cavity to establish a mouse sepsis model. Finally, the mice were subcutaneously injected 1mL saline for post-operative resuscitation. Mice in the sham group underwent laparotomy techniques without ligation and puncture. The ROS scavenger NAC (20 mg/kg) or vehicle was intraperitoneally injected into C57BL/6 mice 1 hour before CLP surgery.
Publication 2023
Abdominal Cavity Betadine Cecum Disinfection Gas Scavengers Intestinal Contents Intestines Laparotomy Ligation Mice, House Mice, Inbred C57BL Needles Operative Surgical Procedures Pulp Canals Punctures Resuscitation Saline Solution Sepsis Sutures
For the gene delivery we used AAV2 serotype that has a high tropism to RGC, long-term expression and low immunogenic profile. The high tropism of AAV2 could be due to the high expression of heparin sulfate proteoglycan by RGC, which mediates attachment of the AAV2 virus. After general and topical anesthesia was administered, the right eye of each animal was rinsed with a 10% povidone-iodine solution (Betadine, EGIS, Hungary). Animals from the experimental group received a 3 μL intravitreal injection of 108 AAV-shRNA-HuR. The control group received 3 μL injections containing 108 AAV-shRNA-scramble control. The injection was performed using a 5 μL Hamilton syringe with a 6 mm-long 34 G needle. The microneedle was gently introduced into the vitreous cavity, avoiding contact with the lens. After injection, the eye was topically treated with 2% chloramphenicol ointment (Detreomycin 2%, Chema-Elektromet, Poland) and secured with a clean dressing. Animals were housed singly in new cages for the next 24 h to avoid injury or infection after the procedure. The intravitreal injection of MT was performed in a similar manner as described above.
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Publication 2023
Animals Animals, Laboratory Antigens Betadine Chloramphenicol Contact Lenses Dental Caries Detreomycin Genes heparin proteoglycan Infection Injuries N-acetyl-S-(1-cyano-2-hydroxyethyl)cysteine Needles Obstetric Delivery Ointments Povidone Iodine Short Hairpin RNA Syringes Topical Anesthetics Tropism Virus Attachment

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Vetbond is a tissue adhesive product manufactured by 3M for use in veterinary applications. It is designed to quickly and effectively bond tissues together, facilitating wound closure and healing. The core function of Vetbond is to provide a reliable and secure means of joining various types of tissue, such as skin, muscle, and membranes, without the need for sutures or other invasive methods.
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Isoflurane is a volatile anesthetic agent used for the induction and maintenance of general anesthesia in veterinary medicine. It is a clear, colorless, and nonflammable liquid with a characteristic, pungent odor. Isoflurane is designed for administration via vaporizer to provide controlled, inhalation anesthesia.
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C57BL/6 mice are a widely used inbred mouse strain commonly used in biomedical research. They are known for their black coat color and are a popular model organism due to their well-characterized genetic and physiological traits.

More about "Betadine"

Betadine, a widely-used topical antiseptic, is a solution containing povidone-iodine that is effective against a broad spectrum of microorganisms, including bacteria, viruses, fungi, and protozoa.
This versatile product is commonly utilized to cleanse and disinfect the skin prior to medical procedures, helping to prevent surgical site infections.
Betadine is also often employed to treat minor cuts, abrasions, and prepare the skin for intravenous access.
Researchers can explore the efficacy and applications of Betadine through PubCompare.ai's innovative AI-driven platform.
This tool facilitates the identification of optimal research protocols and products, empowering researchers to enhance the reproducibility and quality of their studies.
Beyond Betadine, researchers may also be interested in exploring other related products and procedures, such as Rompun (an anesthetic agent), Perma-Hand Silk and 641G Perma-Hand Silk (suture materials), Tegaderm (a transparent dressing), Flunixin meglumine (an anti-inflammatory drug), Bupivacaine (a local anesthetic), Vetbond (a tissue adhesive), Matrigel (a basement membrane extract), Isoflurane (an inhalation anesthetic), and the commonly used C57BL/6 mouse model.
PubCompare.ai's platform can help researchers navigate the vast landscape of research protocols and products, identifying the optimal solutions for their specific needs and enhancing the overall quality and reproducibility of their work.
Experence the power of this innovative tool to take your research to the next level.