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Beuthanasia

Beuthanasia refers to the humane and ethical practice of ending the life of a terminally ill or suffering animal in a painless manner.
This approach aims to minimize animal suffering and provide a dignified end-of-life experience.
Veterinarians and animal welfare professionals follow established protocols and guidelines to ensure the procedure is carried out with the utmost care and compassion.
Beuthanasia is an important consideration in veterinary medicine and animal rescue operations, allowing for a peaceful and dignified transition for animals facing unrelievable pain or an inevitable decline in health.

Most cited protocols related to «Beuthanasia»

Decellularized Porcine Ventricular ECM

Cited 22 times

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Publication 2009

Beuthanasia Blood Vessel Detergents Eosin Euthanasia Freezing Heart Heart Atrium Heart Ventricle Ketamine Phosphates Pigs Powder Saline Solution Sedatives Sulfate, Sodium Dodecyl Tissues Triton X-100 Xylazine

Immunohistochemical Analysis of Phosphorylated α-Synuclein

Cited 5 times

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Publication 2019

Ammonium Animals anti-synaptophysin Antibodies Avidin Beuthanasia Biotin Brain Cell Nucleus Cloning Vectors Common Cold cresyl violet Cryoprotective Agents Drug Overdose Dry Ice Ethanol Freezing Glycol, Ethylene Goat Immunoglobulins Inclusion Bodies Mice, 129 Strain Mice, House Microscopy Microtomy Neurons nickel sulfate hexahydrate Normal Saline paraform Pentobarbital Peroxide, Hydrogen Phosphates Rabbits Rattus Saline Solution Serine Serum Sucrose Triton X-100 Tyrosine 3-Monooxygenase Xylene

Necropsy and Tuberculosis Lesion Analysis

Cited 5 times

Necropsy was performed as previously described [17 (link),24 (link),25 (link),29 (link)]. Briefly, an ¹⁸F-FDG PET-CT scan was performed on every animal 1–3 days prior to necropsy to measure disease progression and identify individual granulomas as described [29 (link)]. At necropsy, monkeys were maximally bled and humanely sacrificed using pentobarbital and phenytoin (Beuthanasia; Schering-Plough, Kenilworth, NJ). Individual lesions previously identified by PET-CT and those that were not seen on imaging from lung and mediastinal lymph nodes were obtained for histological analysis, bacterial burden, and immunological studies [29 (link)]. A veterinary pathologist described gross pathologic findings. To quantify gross pathologic disease (disease burden), a necropsy score was developed in which points were given for TB disease: number, size, and pattern of granulomas distributed in each lung lobe and mediastinal lymph node and in other organs each lung lobe, lymph node, and visceral organ were included and enumerated, and an overall score was determined as previously described [25 (link)]. The size of each granuloma was measured at necropsy and by pre necropsy scan [69 ]. Representative sections of each tissue were homogenized into single-cell suspensions for immunologic studies, flow cytometric analysis, and bacterial burden, as previously described [17 (link),24 (link),26 (link),65 (link)].

Gideon H.P., Phuah J., Myers A.J., Bryson B.D., Rodgers M.A., Coleman M.T., Maiello P., Rutledge T., Marino S., Fortune S.M., Kirschner D.E., Lin P.L, & Flynn J.L. (2015). Variability in Tuberculosis Granuloma T Cell Responses Exists, but a Balance of Pro- and Anti-inflammatory Cytokines Is Associated with Sterilization. PLoS Pathogens, 11(1), e1004603.

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Publication 2015

Animals Autopsy Bacteria Beuthanasia Cells Disease Progression F18, Fluorodeoxyglucose Flow Cytometry Granuloma Lobar Pneumonia Lung Mediastinum Monkeys Nodes, Lymph Pathologic Processes Pathologists Pentobarbital Phenytoin Radionuclide Imaging Scan, CT PET Tissues Vision

Rat Model of Vocal Fold Scarring and bFGF Treatment

Cited 5 times

Twenty four 4-month-old male Sprague Dawley rats were used in this study and assigned to chronic scar, bFGF treated, sham (saline) treated, and control groups. Eight rats underwent unilateral vocal fold stripping with laryngeal harvest 2 months post injury (chronic scar group). Four rats underwent unilateral vocal fold stripping, injection of bFGF 2 months post injury, and laryngeal harvest 4 months post injury (bFGF treated group). Six rats underwent unilateral vocal fold stripping, injection of saline 2 months post injury, and laryngeal harvest 4 months post injury (sham treated group). Six rats underwent no vocal fold stripping or injection (control group).
Vocal fold injuries were created as previously reported (Tateya et al., 2005 ). Rats underwent anesthesia induction with isoflurane (2–3% delivered at 0.8–1.5 L/min) followed by maintenance using an intraperitoneal (IP) injection of ketamine hydrochloride (90 mg/kg) and xylazine hydrochloride (9 mg/kg). Atropine sulfate (0.05 mg/kg) was also injected IP to reduce the secretion of saliva and sputum in the laryngeal lumen. The animals were placed on an operating platform in a near-vertical position and a custom fabricated 1 mm diameter steel wire laryngoscope was inserted to facilitate vocal fold visualization. Vocal fold monitoring was performed using a 1.9 mm diameter 25° endoscope (Richard Wolf, Vernon Hills, IL) connected to an external light source and video monitor. Injuries were created by vocal fold stripping using a 25 G needle and microforceps.
Vocal fold injections were performed using a 50 µL, 50 mm, 26-gauge needle, under endoscopic guidance as described above. bFGF (Sigma-Aldrich, St. Louis, MO) was injected at a concentration of 100 ng in 10 µL; a 100 ng dose was selected based on Hirano et al. (2005) . Normal saline (0.9% w/v NaCl) was injected in a volume of 10 µL.
Euthanasia was performed via intracardiac injection of Beuthanasia (0.22 mg/kg) (Schering-Plough Animal Health, Union, NJ). Larynges were harvested en bloc (Figure 1), quick frozen using liquid nitrogen, and stored at −80°C until use. Larynges from the chronic scar group intended for histological processing were embedded in optimal cutting temperature compound (Tissue-Tek, Sakura, Tokyo, Japan) prior to quick freezing.

Welham N.V., Montequin D.W., Tateya I., Tateya T., Hee Choi S, & Bless D.M. (2009). A Rat Excised Larynx Model of Vocal Fold Scar. Journal of speech, language, and hearing research : JSLHR, 52(4), 1008-1020.

Publication 2008

Anesthesia Animals Beuthanasia Cicatrix Endoscopes Endoscopy Euthanasia Injections, Intraperitoneal Injuries Isoflurane Ketamine Hydrochloride Laryngeal Prosthesis Laryngoscopes Larynx Light Males Needles Nitrogen Normal Saline Rats, Sprague-Dawley Rattus norvegicus Saline Solution Salivation Sputum Steel Sulfate, Atropine Tissues Vocal Cords Wolves Xylazine Hydrochloride

Microglia/Macrophage IHC Analysis Protocol

Cited 4 times

Animals and tissues were processed for IHC as described in our previous study (22 (link)). The tissues used for IHC and densitometry analysis in Schartz et al. (22 (link)) were further analyzed for microglia/macrophage cell and morphology counts in the current study. Briefly, animals were anesthetized with beuthanasia (200 mg/kg) and perfused with ice cold 1× PBS followed by 4% paraformaldehyde (PFA). After overnight post-fixation (4%-PFA) and cryoprotection (30% sucrose), brains were frozen in pre-chilled isopentane and stored at −80°C until used. Coronal brain sections (50 µm) were stored in 1×PBS + 0.1% sodium azide at 4°C. Serial sections from rostral to caudal (4–6 sections per brain) were collected at approximately every 500 µm along the dorsoventral axis between the Bregma coordinates −3.00 mm and −5.28 mm. These sections were immunostained with anti-rabbit IBA1 (1:500; Wako Chemicals Cat# 019-19741, RRID: AB_839504) followed by biotinylated goat anti-rabbit secondary antibodies (1:2,000; Vector Laboratories Cat# BA-1000 RRID:AB_2313606), incubated in ABC avidin/biotin complex solution and developed using the DAB Peroxidase (HRP) Substrate Kit, 3,3′-diaminobenzidine (Vector Laboratories). Brain sections were mounted on gelatin-coated slides, Nissl stained, dehydrated in alcohol, de-fatted in Xylene, and coverslipped using Permount mounting media. All chemicals were obtained from Fisher Scientific unless otherwise indicated.

Wyatt-Johnson S.K., Herr S.A, & Brewster A.L. (2017). Status Epilepticus Triggers Time-Dependent Alterations in Microglia Abundance and Morphological Phenotypes in the Hippocampus. Frontiers in Neurology, 8, 700.

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Publication 2017

Animals Anti-Antibodies Avidin Beuthanasia Biotin Brain Cloning Vectors Cold Temperature Densitometry Epistropheus Ethanol Freezing Gelatins Goat isopentane Macrophage Microglia paraform Peroxidase Rabbits Sodium Azide Sucrose Tissues Xylene

Most recents protocols related to «Beuthanasia»

Mapping Melanocortin Receptor Neurons

Subjects were injected with a lethal dose of sodium pentobarbital (Beuthanasia-D, 0.25 ml i.p., Schering, Union, NJ, United States) and sacrificed by rapid decapitation. Brains were extracted, frozen in optimum cutting temperature compound (VWR Scientific Products) and stored at −80°C. Brains were later sliced coronally on a cryostat at 14 μm thickness and sections were mounted on Colorfrost Plus slides (Fisher Scientific, Waltham, MA, United States). Some studies in rats and mice have provided an extensive analysis of range of the distribution of melanocortin receptor expressing neurons. Here, we limited our focus to the forebrain and midbrain with tissue collected from slices containing the prefrontal cortex through to slices containing the dorsal raphe nucleus. Slides were stored at −80°C until RNAscope procedure treatment.

Hall M.A., Kohut-Jackson A.L., Peyla A.C., Friedman G.D., Simco N.J., Borland J.M, & Meisel R.L. (2023). Melanocortin receptor 3 and 4 mRNA expression in the adult female Syrian hamster brain. Frontiers in Molecular Neuroscience, 16, 1038341.

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Publication 2023

Beuthanasia Brain Decapitation Freezing Melanocortin Receptor Mesencephalon Mice, Laboratory Neurons Pentobarbital Sodium Prefrontal Cortex Prosencephalon Raphe, Dorsal Nucleus Rattus Tissues

Prenatal ENDS Exposure and Offspring Asthma

For this in vivo study, we used pregnant 12-week-old female BALB/c mice (Charles River, Wilmington, MA, USA). These mice were exposed in two separate sessions to either filtered air or vanilla-flavored ENDS aerosols for 2 h per day for 20 consecutive days during gestation. The exposures of the pregnant mice to ENDS aerosols were conducted as previously described [22 (link),23 (link),28 (link)]. In total, 15 dams exposed to air and 9 dams exposed to vanilla-flavored e-cig aerosol gave birth, with similar average litter sizes of 6.93 and 6.44 pups, respectively. The average birth weight of the pups was also similar, with 1.39 g and 1.40 g for the pups exposed in utero to air and e-cig aerosol, respectively. We controlled for ‘litter effects’ by using one offspring per sex per litter for the biological endpoint analyzed. At birth, sub-groups of mouse offspring were euthanized by using an intraperitoneal injection of Beuthanasia-D (Schering-Plough, Kenilworth, NJ, USA), followed by decapitation. The lungs of those 1-day-old offspring were excised and stored in RNAlater, for subsequent RNA sequencing analysis. After weaning (4 weeks of age), additional sub-groups of male mouse offspring were treated with intranasal instillation of either HDM in order to induce asthmatic responses or saline for the control treatment. Intranasal instillations were carried out once a week for 3 consecutive weeks. At 7 weeks of age, following the completion of the HDM or saline treatment, the male offspring were euthanized by using an intraperitoneal injection of Beuthanasia-D (Schering-Plough, NJ, USA). The samples were collected and stored for subsequent analyses. All of the mice were housed in an AAALAC-approved animal care facility at the Louisiana State University School of Veterinary Medicine under a 12 h light/dark cycle (from 6:00 a.m. to 6:00 p.m.). The mice had access to water and food ad libitum, except during the 2 h exposure periods. The mice were housed and handled in accordance with the NIH Guide for the Care and Use of Laboratory Animals. All of the procedures and protocols were approved by the Louisiana State University Institutional Animal Care and Use Committee.

Noël A., Yilmaz S., Farrow T., Schexnayder M., Eickelberg O, & Jelesijevic T. (2023). Sex-Specific Alterations of the Lung Transcriptome at Birth in Mouse Offspring Prenatally Exposed to Vanilla-Flavored E-Cigarette Aerosols and Enhanced Susceptibility to Asthma. International Journal of Environmental Research and Public Health, 20(4), 3710.

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Publication 2023

Animals Animals, Laboratory Asthma Beuthanasia Biopharmaceuticals Birth Birth Weight Decapitation Food Injections, Intraperitoneal Institutional Animal Care and Use Committees Lung Men Mice, House Mice, Inbred BALB C Pregnancy Pregnant Women Rivers Saline Solution Sequence Analysis Uterus Vanilla

Post-Mortem Heart Imaging Protocol

The animals were euthanized using intravenous Beuthanasia and their hearts were extracted and imaged with an average of 107 days after the inducibility study as reported in supplementary material. After washing the hearts with sodium chloride and cleaning and cutting the fat around the heart, they were placed in cold phosphate-buffered saline (PBS). We then prepared the hearts for imaging by filling them with dental alginate mix through the pulmonary veins and superior and inferior vena cava to keep the atrial geometry and shape intact and prevent collapsing of the atrial wall. We then soaked them in fomblin to get rid of any trapped bubbles. After taking them out of fomblin, we placed them in 4% buffered formalin and stored them at room temperature at least 24 hours prior to MR imaging. The hearts that were not imaged right away were filled with alginate and stored in 10% neutral buffered formalin for a week. After a week of fixation, the hearts were stored in PBS in a refrigerator.

Kamali R., Kwan E., Regouski M., Bunch T.J., Dosdall D.J., Hsu E., Macleod R.S., Polejaeva I, & Ranjan R. (2023). Contribution of atrial myofiber architecture to atrial fibrillation. PLOS ONE, 18(1), e0279974.

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Publication 2023

Alginate Animals Beuthanasia Cold Temperature Dental Health Services Formalin Heart Heart Atrium NOS2A protein, human Phosphates Saline Solution Sodium Chloride Veins, Pulmonary Vena Cavas, Inferior

Evaluating Cortical Lesion Volume

Immediately following the terminal procedure, the rats were injected with (Beuthanasia-D) and transcardially perfused with 0.1M saline solution with heparin and lidocaine followed by 4% paraformaldehyde. The brains were extracted, gelatin embedded, and sectioned coronally at a thickness of 50-μm using a freezing, sliding microtome. The sections were mounted on slides and stained with cresyl violet. An example of coronal hemisections corresponding to CFA are shown with examples of the small, subtotal lesions generated in these experiments is shown in Figure 2. Sections were analyzed under a light microscope (Zeiss AxioImager M2) and sampled at 1.2-mm intervals. Hemispheric volume through the sensorimotor cortex was estimated using the Cavalieri method, labelling the region of interest, which was drawn around gaps and non-viable tissue in the injured cortex to outline the lesion area, with markers (Figure 2B).

Hayley P., Tuchek C., Dalla S., Borrell J., Murphy M., Nudo R, & Guggenmos D. (2023). Post-Ischemic Reorganization of Sensory Responses in Cerebral Cortex. bioRxiv.

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Publication Preprint 2023

Beuthanasia Brain Cortex, Cerebral cresyl violet Gelatins Heparin Lidocaine Light Microscopy Microtomy paraform Rattus Saline Solution Sensorimotor Cortex Tissues

Biomechanical Phenotyping of Progeria Mice

All live animal procedures were approved by the Yale IACUC. Lmna^G609G/G609G (progeria) mice were generated by breeding Lmna^G609G/+ mice, which yielded wild-type Lmna^+/+ (WT) mice as littermate controls, noting that these mice are on a C57BL/6 background. Male and female mice were euthanized with an intraperitoneal injection of Beuthanasia-D at the appropriate postnatal age (P42, P100, P140, or P168), and five arterial segments (ATA, DTA, CCA, ICA, and MA) were gently excised and prepared for biomechanical phenotyping, with a sixth segment (abdominal aorta) included for RNA sequencing, noting that changes in the thoracic and abdominal aorta are comparable in progeria and independent of sex (Murtada et al., 2020 (link)). We thus report mixed-sex findings herein. In all cases, death was confirmed by loss of cardiovascular function following exsanguination. Finally, ATA, DTA, and ICA segments were similarly excised from separate C57BL/6J WT mice at P168 and P850 (~2.3 years of natural aging) for comparison.

Murtada S.I., Kawamura Y., Cavinato C., Wang M., Ramachandra A.B., Spronck B., Tellides G, & Humphrey J.D. (2023). Smooth Muscle Cell Death Drives an Osteochondrogenic Phenotype and Severe Proximal Vascular Disease in Progeria. bioRxiv.

Publication Preprint 2023

Animals Aortas, Abdominal Arteries Beuthanasia Cardiovascular Physiological Phenomena Exsanguination Injections, Intraperitoneal Institutional Animal Care and Use Committees Males Mice, House Mice, Inbred C57BL Progeria TPX2 protein, human Woman

Top products related to «Beuthanasia»

Beuthanasia d by Merck Group

Sourced in United States

Beuthanasia-D is a laboratory equipment used for the humane euthanasia of laboratory animals. It is designed to provide a rapid and painless means of ending the life of research animals. The product functions by administering a barbiturate solution that induces deep anesthesia and cardiac arrest, resulting in a peaceful and humane death.

Beuthanasia d by Merck & Co

Sourced in United States

Beuthanasia-D is a laboratory equipment product manufactured by Merck & Co. It is a euthanasia solution intended for use in research and veterinary settings.

Beuthanasia by Merck Group

Sourced in United States

Beuthanasia is a laboratory equipment product. It is used for the humane euthanasia of laboratory animals. The core function of Beuthanasia is to provide a controlled and painless method of ending the life of laboratory animals when necessary.

Beuthanasia d special by Merck Group

Sourced in United States

Beuthanasia-D Special is a laboratory equipment product manufactured by Merck Group. It is designed for the purpose of euthanasia. The core function of this product is to facilitate the humane and controlled termination of life, specifically for research or medical applications.

Beuthanasia by Merck & Co

Beuthanasia is a laboratory equipment product manufactured by Merck & Co. It is designed for the purpose of euthanasia, a controlled and humane method of ending the life of an animal. The core function of Beuthanasia is to provide a safe and effective means to perform this procedure.

Paraformaldehyde (pfa) by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Germany, China, France, Australia, Canada, India, Belgium, Japan, Morocco, Switzerland

Paraformaldehyde is a chemical compound used as a preservative and fixative in various laboratory applications. It is a solid, white, crystalline substance that slowly releases formaldehyde gas. Paraformaldehyde is commonly used to fix and preserve biological samples, such as cells and tissues, for microscopic examination and analysis.

Beuthanasia d by Performance Health

Beuthanasia-D is a laboratory equipment product. It is used for the purpose of euthanasia.

Oct compound by Sakura Finetek

Sourced in Japan, United States, Netherlands, Germany, Switzerland, United Kingdom, France

The OCT compound is a specialized laboratory equipment designed for optical coherence tomography (OCT) analysis. It enables high-resolution, non-invasive imaging of biological samples by utilizing low-coherence interferometry. The core function of the OCT compound is to capture detailed, cross-sectional images of various materials and tissues.

Superfrost plus by Thermo Fisher Scientific

Sourced in United States, Germany, United Kingdom, Spain, Canada, Australia, France

Superfrost Plus is a microscope slide product manufactured by Thermo Fisher Scientific. It is designed to provide a superior adhesive surface for the attachment of tissue sections and cell samples during histological and cytological analyses.

Beuthanasia d pentobarbital solution by Cardinal Health

Beuthanasia-D is a pentobarbital sodium solution for euthanasia of animals. It is a sterile, nonpyrogenic solution intended for intravenous administration.

What are the different types of Beuthanasia procedures?

There are several common Beuthanasia methods used in veterinary medicine, including intravenous injection of barbiturates, inhalation of gases like carbon monoxide or nitrogen, and occasionally physical methods like gunshot or captive bolt. The specific technique chosen depends on factors like the animal's size, temperament, and health condition, as well as the expertise of the veterinarian or animal care professional.

How can PubCompare.ai help optimize the use of Beuthanasia protocols?

PubCompare.ai allows researchers to more efficiently screen the literature on Beuthanasia protocols and leverage AI to identify critical insights. The platform can help pinpoint the most effective Beuthanasia methods for your specific research goals, highlighting key differences in protocol effectiveness so you can choose the best option for reproducibility and accuracy. This can be especially helpful when dealing with sensitive procedures like Beuthanasia, where having the right approach is crucial.

What are some common challenges in performing Beuthanasia properly?

Ensuring a humane and pain-free end-of-life experience is critical when performing Beuthanasia. Common challeges include properly sedating or restraining the animal, administering the euthanasia agent correctly, and confirming death has occurred. Veterinarians and staff must also be mindful of the emotional toll on owners and themselves. Following established protocols and guidelines is essential to overcome these hurdles and provide the best possible care.

How does Beuthanasia differ from other end-of-life options for animals?

Unlike other end-of-life options like natural death or euthanasia, Beuthanasia is a controlled process carried out by trained professionals to end an animal's suffering in a humane and painless manner. This approach is considered more ethical and compassionate than allowing a terminally ill or suffering animal to endure a prolonged and potentially painful death. Beuthanasia is a vital tool in veterinary medicine and animal welfare to ensure a dignified transition for the animal.

More about "Beuthanasia"

Beuthanasia, also known as euthanasia in veterinary medicine, refers to the humane and ethical practice of ending the life of a terminally ill or suffering animal in a painless manner.
This approach aims to minimize animal suffering and provide a dignified end-of-life experience.
Veterinarians and animal welfare professionals follow established protocols and guidelines, such as those outlined in Beuthanasia-D, Beuthanasia, and Beuthanasia-D Special, to ensure the procedure is carried out with the utmost care and compassion.
Beuthanasia is an important consideration in veterinary medicine and animal rescue operations, allowing for a peaceful and dignified transition for animals facing unrelievable pain or an inevitable decline in health.
The process may involve the use of substances like pentobarbital solution, paraformaldehyde, or OCT compound, which are designed to provide a quick and painless death.
Superfrost Plus slides may also be utilized in the process to ensure proper tissue preservation.
By understanding the protocols and guidelines surrounding beuthanasia, pet owners and animal care professionals can make informed decisions that prioritize the well-being and comfort of the animals in their care.
This can help to alleviate the suffering of terminally ill or severely distressed animals, providing them with a dignified and humane end-of-life experience.