The 6530 QTOF mass spectrometer for measurement of reference compounds was operated with the following parameters. An Agilent JetStream electrospray source was used in infusion mode at a flow rate of 0.25 ml/min for acquiring QTOF MS and MS/MS spectra. Data were collected with a 0.25 s scan rate in both profile and centroid modes, and mass calibration was maintained by constant infusion of reference ions at 121.0509 and 922.0098 m/z. MS/MS data was generated utilizing data-dependent MS/MS triggering with dynamic exclusion. Precursor ions, with a minimum 1 k signal intensity were isolated with a 4 m/z isolation width (medium setting), and a variable collision energy was applied based on precursor ion m/z (10 eV + 0.03 eV × ion m/z). Data were exported into the open exchange format mzXML. Samples were measured in negative and positive mode. For lipid profiling with liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-MS/MS) we used settings from an external reference25 (link), except we choose a scan rate of 4-8 spectra per scan event and collision energies ranging from 20-40eV.
Butyl acetate
It is commonly used as a solvent, in lacquers, varnishes, and other coatings, as well as in the manufacture of perfumes and flavors.
Butyl acetate is easily absorbed through the skin and can cause irritation and drowsiness if inhaled or ingested in large quantities.
Researchers can use PubCompare.ai's AI-powered platform to optimize their protocols for reproducibility and accuracy when working with this chemical, exploring the latest literature, pre-prints, and patents to find the best procedures and products.
Most cited protocols related to «Butyl acetate»
The 6530 QTOF mass spectrometer for measurement of reference compounds was operated with the following parameters. An Agilent JetStream electrospray source was used in infusion mode at a flow rate of 0.25 ml/min for acquiring QTOF MS and MS/MS spectra. Data were collected with a 0.25 s scan rate in both profile and centroid modes, and mass calibration was maintained by constant infusion of reference ions at 121.0509 and 922.0098 m/z. MS/MS data was generated utilizing data-dependent MS/MS triggering with dynamic exclusion. Precursor ions, with a minimum 1 k signal intensity were isolated with a 4 m/z isolation width (medium setting), and a variable collision energy was applied based on precursor ion m/z (10 eV + 0.03 eV × ion m/z). Data were exported into the open exchange format mzXML. Samples were measured in negative and positive mode. For lipid profiling with liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-MS/MS) we used settings from an external reference25 (link), except we choose a scan rate of 4-8 spectra per scan event and collision energies ranging from 20-40eV.
Most recents protocols related to «Butyl acetate»
Samples of packing materials covered with the biofilm were taken at a depth of 20 cm from the top of each biofilter. A standard sample procedure was applied previously published for CFM analyses. [16] (link) To observe the complex bioreactor ecology, the entire volume, 50 mL of BSM medium, was applied at once to each biofilter to wash out any possible protozoa, metazoa and mite. The leachate was collected and examined by microscopy using an Olympus BX40 microscope equipped with a Canon EOS 700D camera. Samples were taken on 119th and 300th days of the biofilter operation.
The experimental apparatus consisted of standard units providing the inlet air humidification, the liquid pollutant being dosed into air using a syringe pump, NE-500 (New Era Pump Systems Inc., USA) equipped with a 500 mL glass syringe (Setonic Inc., Germany). The system also contained an airborne ethyl acetate detector, VOC-TRAQ flow cell (AMETEK MOCON, USA) equipped with a Purple piD-TECH eVx photoionization sensor (AMETEK MOCON, USA), and a BSM medium dosage device with programmable peristaltic pumps, LC-Cube PPS-9K6 (Watrex Inc., Czech Republic). The biofilter bed pressure drop measured in Pa was determined using a U-shaped tube differential pressure manometer on 0 (dry packing), 1st (wet packing), 38th, 115th and 300th (last) days of the biofilter operation. n-Butyl acetate (PENTA Ltd., pure grade with minimum 99% content) was selected as a representative contaminant to simulate the real-world broad-range mixture of volatile odorous fatty acids and their esters emitted from a typical textile plant, Juta a.s. (https://www.juta.eu/, Czech Republic). The effect of loading types on the biofilter performance was tested for 300 days (Table 1). Two loading patterns were applied. One of them included the loading variation by air flow rate (V g , in a range of 8-24 L min -1 at C in of 500 and 100 mg m -3 ) to simulate waste air with a low pollutant concentration yet causing a pungent odor). The second loading pattern was based on the variation of inlet concentration (C in , in a range of 100-1500 mg m -3 at V g of 3, 6 and 12 L min -1 ) to simulate heavily polluted air with relatively low flow rates. The biofiltration performance was evaluated using standard biofiltration parameters, elimination capacity, organic load, removal efficiency and empty bed residence time defined as follows:
Elimination capacity (EC):
Organic load (OL):
Removal efficiency (RE): Empty bed retention time (EBRT):
where C in and C out are the inlet and outlet n-butyl acetate concentrations in air (g m -3 ), respectively; V b is the packed bed volume (m 3 ) and Q is the air flow rate (m 3 h -1 ).
A mixed enriched culture taken from a biofilter treating a mixture of acetone and styrene, which was defined and successfully used previously [12] (link) was applied as an inoculum. The inoculum contained culturable isolated and identified bacteria, Pseudomonas sp., Bacillus sp., Arthrobacter sp., yeasts, Rhodococcus sp., Komagataella pastoris and fungus Fusarium solani. The selected mineral medium composition (BSM) allowed one to control pH (as a phosphate buffer) and supply the biofilm with water and mineral nutrients. 30 mL of this medium were added twice a day to each biofilter. It had the following composition (concentration given in g � L -1 ): KH 2 PO 4 (2.3), K 2 HPO 4 (2.9), (
Example 62
Step 1: tert-butyl 2-(4-(7-chloro-4-(1H-imidazol-1-yl)quinolin-2-yl)-2-oxo-1,4-diazepan-1-yl)acetate. To a solution of 4-(7-chloro-4-(1H-imidazol-1-yl)quinolin-2-yl)-1,4-diazepan-2-one (20 mg) and tert-butyl 2-bromoacetate (30 mg) in anhydrous DMF was added NaH (10 mg, 65% in mineral oil). After stirring 3 hours, the reaction mixture was diluted with EtOAc (10 mL) and carefully quenched with water (5 mL). Isolation of the organic layer and a column chromatography eluting with a gradient of hexanes and EtOAc afforded the desired intermediate tert-butyl 2-(4-(7-chloro-4-(1H-imidazol-1-yl)quinolin-2-yl)-2-oxo-1,4-diazepan-1-yl)acetate (20 mg) (MS: [M+1]+ 456).
Step 2: 2-(4-(7-chloro-4-(1H-imidazol-1-yl)quinolin-2-yl)-2-oxo-1,4-diazepan-1-yl)acetic acid. tert-butyl 2-(4-(7-chloro-4-(1H-imidazol-1-yl)quinolin-2-yl)-2-oxo-1,4-diazepan-1-yl)acetate was further treated with TFA (0.4 mL) in DCM (0.8 mL). Removal of DCM and TFA under reduced pressure and lyophilization afforded the desired product (10 mg)-2-(4-(7-chloro-4-(1H-imidazol-1-yl)quinolin-2-yl)-2-oxo-1,4-diazepan-1-yl)acetic acid (MS: [M+1]+ 400).