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Calcium acetate

Calcium acetate, a versatile compound with a wide range of applications in research and industry.
This chemical substance is known for its use in a variety of processes, including the production of pharmaceuticals, food additives, and industrial materials.
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Most cited protocols related to «Calcium acetate»

1
Olfactory Neuronal Calcium Imaging in Flies
Cited 50 times
Flies were reared on standard cornmeal agar medium. We used the Gal4/UAS system 41 (link) to direct the expression of the calcium sensors to PNs. GH146-Gal4 flies were a gift from L. Luo (Stanford University, Stanford, CA). UAS-GCaMP1.6 flies were a gift from D. Reiff and A. Borst (MPI, Martinsried, Germany). All experimental animals were adult females, 3–5 days after eclosion. Adult flies were dissected using previously described methods 11 (link). Flies were anaesthetized in a vial on ice just until movement stopped (<15 second) and then gently inserted into a hole in a piece of aluminum foil. Small drops of wax (55°C) were used to suspend the fly in the hole, with the edge of foil defining a horizontal plane around the head and thorax, from the first antennal segment anteriorly to the scutellum posteriorly. The dorsal side of the foil was bathed in saline, while the ventral side (including antennae and maxillary palps) remained dry and accessible to odours. A window was cut in the dorsal head cuticle between the eyes, extending from the ocelli to the first antennal segment. Fat and air sacs dorsal and anterior to the brain were removed, but the perineural sheath was left intact. The proboscis was affixed with a small drop of wax to a strand of human hair to limit brain movement. Spontaneous leg movements were typically observed in this preparation for the duration of the recording (1.5–3 hr). The saline composition used in all experiments was 42 (link) (in mM): 103 NaCl,3 KCl, 5 N-tris(hydroxymethyl) methyl-2-aminoethane-sulfonicacid, 10 trehalose, 10 glucose, 2 sucrose, 26 NaHCO3, 1 NaH2PO4,1.5 CaCl2, and 4 MgCl2, adjusted to 275 mOsm, pH 7.3 when bubbled with 95% O2/5%CO2.
Odours (cis-3-hexen-1-ol (cis), and isoamyl acetate (ia)) were delivered using a custom-made odour-delivery system and a Teflon nozzle (entry diameter 1/8″) directed towards the antennae. Odours were delivered in a constant stream of air (1 l/min) at final concentrations of ca. 15%. Odour delivery times were measured using a mini-PID (Aurora Scientific Inc., Ontario, Canada). Odours were presented for either 3s or 5s. All comparisons of sensor performance were made using experiments with identical odour presentation times. The results reported are based on data obtained from 3 GCaMP1.6-expressing flies (4 ALs) and 4 GCaMP3-expressing flies (4 ALs).
Tian L., Hires S.A., Mao T., Huber D., Chiappe M.E., Chalasani S.H., Petreanu L., Akerboom J., McKinney S.A., Schreiter E.R., Bargmann C.I., Jayaraman V., Svoboda K, & Looger L.L. (2009). Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators. Nature methods, 6(12), 875-881.
Publication 2009
2
Structural Determination of CRISPR-Cas9 Complexes
Cited 31 times
Full details of experimental procedures are provided in the supplementary materials. SpyCas9 and its point mutants were expressed in Escherichia coli Rosetta 2 strain and purified essentially as described (8 (link)). SpyCas9 crystals were grown using the hanging drop vapor diffusion method from 0.1 M tris-Cl (pH 8.5), 0.2 to 0.3 M Li2SO4, and 14 to 15% (w/v) PEG 3350 (polyethylene glycol, molecular weight 3350) at 20°C. Diffraction data were measured at beamlines 8.2.1 and 8.2.2 of the Advanced Light Source (Lawrence Berkeley National Laboratory), and at beamlines PXI and PXIII of the Swiss Light Source (Paul Scherrer Institut) and processed using XDS (50 (link)). Phasing was performed with crystals of selenomethionine (SeMet)–substituted SpyCas9 and native Cas9 crystals soaked individually with 10 mM Na2WO4, 10 mM CoCl2, 1 mM thimerosal, and 1 mM Er(III) acetate. Phases were calculated using autoSHARP (51 (link)) and improved by density modification using Resolve (52 (link)). The atomic model was built in Coot (53 (link)) and refined using phenix.refine (54 (link)).
A. naeslundii Cas9 (AnaCas9) was expressed in E. coli Rosetta 2 (DE3) as a fusion protein containing an N-terminal His10 tag followed by MBP and a TEV (tobacco etch virus) protease cleavage site. The protein was purified by Ni-NTA (nickel–nitrilotriacetic acid) and heparin affinity chromatography, followed by a gel filtration step. Crystals of native and SeMet-substituted AnaCas9 were grown from 10% (w/v) PEG 8000, 0.25 M calcium acetate, 50 mM magnesium acetate, and 5 mM spermidine. Native and SeMet single-wavelength anomalous diffraction (SAD) data sets were collected at beamline 8.3.1 of the Advanced Light Source, processed using Mosflm (55 (link)), and scaled in Scala (56 (link)). Phases were calculated in Solve/Resolve (52 (link)), and the atomic model was built in Coot and refined in Refmac (57 (link)) and phenix.refine (54 (link)).
For biochemical assays, crRNAs were synthesized by Integrated DNA Technologies, and tracrRNA was prepared by in vitro transcription as described (8 (link)). The sequences of RNA and DNA reagents used in this study are listed in table S2. Cleavage reactions were performed at room temperature in reaction buffer [20 mM tris-Cl (pH 7.5), 100 mM KCl, 5 mM MgCl2, 5% glycerol, 1 mM dithiothreitol] using 1 nM radio-labeled dsDNA substrates and 1 nM or 10 nM Cas9:crRNA:tracrRNA. Cleavage products were resolved by 10% denaturing (7 M urea) PAGE and visualized by phosphorimaging. Cross-linked peptide-DNA heteroconjugates were obtained by incubating 200 pmol of catalytically inactive (D10A/H840A) Cas9 with crRNA:tracrRNA guide and 10-fold molar excess of BrdU containing dsDNA substrate for 30 min at room temperature, followed by irradiation with UV light (308 nm) for 30 min. S1 nuclease and phosphatase–treated tryptic digests were analyzed using a Dionex UltiMate3000 RSLCnano liquid chromatograph connected in-line with an LTQ Orbitrap XL mass spectrometer equipped with a nanoelectrospray ionization source (Thermo Fisher Scientific).
For negative-stain EM, apo-SpyCas9, SpyCas9: RNA, and SpyCas9:RNA:DNA complexes were reconstituted in reaction buffer, diluted to a concentration of ~25 to 60 nM, applied to glow-discharged 400-mesh continuous carbon grids, and stained with 2% (w/v) uranyl acetate solution. Data were acquired using a Tecnai F20 Twin transmission electron microscope operated at 120 keV at a nominal magnification of either ×80,000 (1.45 Å at the specimen level) or ×100,000 (1.08 Å at the specimen level) using low-dose exposures (~20 e− Å−2) with a randomly set defocus ranging from −0.5 to −1.3 μm. A total of 300 to 400 images of each Cas9 sample were automatically recorded on a Gatan 4k × 4k CCD (charge-coupled device) camera using the MSI-Raster application within the automated macromolecular microscopy software Leginon (58 (link)). Particles were preprocessed in Appion (45 (link)) before projection matching refinement with libraries from EMAN2 and SPARX (59 (link), 60 (link)) using RCT reconstructions (34 (link)) as initial models.
Jinek M., Jiang F., Taylor D.W., Sternberg S.H., Kaya E., Ma E., Anders C., Hauer M., Zhou K., Lin S., Kaplan M., Iavarone A.T., Charpentier E., Nogales E, & Doudna J.A. (2014). Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation. Science (New York, N.Y.), 343(6176), 1247997.
Publication 2014
3
Multimodal Analysis of Visual Cortex Circuitry
Cited 31 times
We performed two-photon imaging in the mouse visual cortex as described previously27 (link),30 (link) by recording calcium responses to visual stimuli consisting of drifting gratings in each of 16 directions. We then acquired an in vivo fluorescent anatomical volume after injecting the tail vein with SR101 (100 mM) to label vasculature. The animal was perfused transcardially (2% paraformaldehyde/2.5% glutaraldehyde) and the brain was processed for serial-section TEM. Serial thin (<50 nm) sections were cut, picked up on pioloform-coated slot grids, and then post-stained with uranyl acetate and lead citrate. 1,215 serial sections were imaged at 120 kV on a JEOL 1200 EX with a custom scintillator atop optical-quality leaded vacuum glass at the end of a custom-built vacuum chamber extension. Custom software controlled automated x–y stage motion and image acquisition with a 2x2 array of CCD cameras (Imperx IPX-11M5) and Zeiss lenses. Images suitable for circuit reconstruction were acquired at a net rate of 5–8 MPix/s. Camera images were aligned in 2–D by registering adjacent camera images and dewarping, followed by histogram equalization and stitching. Then adjacent sections were registered and 3-D deformations were equalized in aligning the EM volume. Axonal and dendritic arbours of the functionally characterized neurons were manually reconstructed using TrakEM2 and objects were classified using classical criteria33 . Neurons or dendritic fragments receiving synapses from multiple functionally characterized cells were included in analysis of convergence. For each synapse participating in a convergence, a second individual (blind to the original reconstruction) traced the pre- and the post-synaptic processes, starting from the synapse. Segmentation that diverged between the two tracers was excluded from further analysis. Cumulative synaptic proximity (CSP) of pairs of axons was calculated by centring a 3-D Gaussian density function at each synaptic bouton and taking the sum of their dot products over all pairs of synapses.
Bock D.D., Lee W.C., Kerlin A.M., Andermann M.L., Hood G., Wetzel A.W., Yurgenson S., Soucy E.R., Kim H.S, & Reid R.C. (2011). Network anatomy and in vivo physiology of visual cortical neurons. Nature, 471(7337), 177-182.
Publication 2011
Animals Axon Brain Calcium Cells Citrates Dendrites Glutaral Lens, Crystalline Mus Neurons paraform pioloform Presynaptic Terminals Reconstructive Surgical Procedures Synapses Tail uranyl acetate Vacuum Veins Vision Visual Cortex Visually Impaired Persons
4
Phytochemical Profiling of Acacia nilotica Pods
Cited 24 times

Plant collection and identification. Fresh pods of A. nilotica were collected in June 2008 from Potiskum, Yobe State, Nigeria. The pods were identified by a taxonomist in Department of Biological Sciences, University of Maiduguri, Maiduguri, Nigeria. The pods were air dried for three weeks under the shade and ground into fine powder.
Preparation of aqueous extract. Three hundred and fifty grams (350 g) of the powdered extract sample were exhaustively extracted with distilled water using reflux method. The crude aqueous extract was concentrated in vacuo and a brown colored extract weighing two hundred and sixty three grams (263 g) w/w was obtained. It was thereafter stored in a refrigerator at 4 ˚C until used.4 Fractionation of the aqueous pod extract. The method used for fractionation of A. nilotica pod powder has already been reported.5 (link),6 The crude aqueous pod extract was suspended in cold distilled water and then filtered using Whatman filter paper. The filtrate was thereafter subjected to fractionation using, chloroform, ethyl acetate and n-butanol. The fractionation with the organic solvents of different polarity was done until the organic layers were visibly clear to obtain ethyl acetate (58 g), n-butanol (25 g) soluble fractions and the residue (180 g). The product did not dissolve in chloroform, hence no product was obtained as shown in Fig. 1.
Phytochemicalanalysis of theextracts of A.nilotica. The aqueous extract and ethyl acetate, N-butanol and residual fractions of A. nilotica extracts were subjected to qualitative chemical screening for identification of various classes of active chemical constituents.7 , 9 Test for tannins (Ferric chloride test). Two millilitres (2 mL) of the aqueous solution of the extract were added to a few drops of 10% Ferric chloride solution (light yellow). The occurrence of blackish blue colour showed the presence of gallic tannins and a green-blackish colour indicated presence of catechol tannins.
Test for saponins (Frothing Test). Three millilitres (3 mL) of the aqueous solution of the extract were mixed with 10 mL of distilled water in a test-tube. The test-tube was stoppered and shaken vigorously for about 5 min, it was allowed to stand for 30 min and observed for honeycomb froth, which was indicative of the presence of saponins.
Test for alkaloids. One gram (1 g) of the extract was dissolved in 5 mL of 10% ammonia solution and extracted with 15 mL of chloroform. The chloroform portion was evaporated to dryness and the resultant residue dissolved in 15 mL of dilute sulphuric acid. One quarter of the solution was used for the general alkaloid test while the remaining solution was used for specific tests.
Mayer’s reagent (Bertrand’s reagent). Drops of Mayer’s reagent was added to a portion of the acidic solution in a test tube and observed for an opalescence or yellowish precipitate indicative of the presence of alkaloids.
Dragendorff’s reagent. Two millilitres (2 mL) of acidic solution in the second test-tube were neutralized with 10% ammonia solution. Dragendorff’s reagent was added and turbidity or precipitate was observed as indicative of presence of alkaloids.
Tests for carbohydrate (Molisch’s test). A few drops of Molischs solution was added to 2 mL of aqueous solution of the extract, thereafter a small volume of concentrated sulphuric acid was allowed to run down the side of the test tube to form a layer without shaking. The interface was observed for a purple colour as indicative of positive for carbohydrates.
Testsforcarbohydrate (Barfoed’stest). One milliliter (1 mL) of aqueous solution of the extract and 1ml of Barfoed’s reagent were added into a test-tube, heated in a water bath for about 2 min. Red precipitate showed the presence of monosaccharaides.
Standard test for combined reducing sugars. One milliliter (1 mL) of the aqueous solution of the extract was hydrolyzed by boiling with 5 mL of dilute hydrochloric acid (HCl). This was neutralized with sodium hydroxide solution. The Fehling’s test was repeated as indicated above and the tube was observed for brick-red precipitate that indicated the presence of combine reducing sugars.
StandardtestforfreereducingSugar (Fehling’stest). Two milliliters (2 mL) of the aqueous solution of the extract in a test tube was added into 5 mL mixture of equal volumes of Fehling’s solutions I and II and boiled in a water bath for about 2 min. The brick-red precipitate was indicative of the presence of reducing sugars.
Test for ketones. Two millilitres (2 mL) of aqueous solution of the extract were added to a few crystals of resorcinol and an equal volume of concentrated HCl, and then heated over a spirit lamp flame and observed for a rose colouration that showed the presence of ketones.
Testforpentoses. Two millilitres (2 mL) of the aqueous solution of the extract were added into an equal volume of concentrated HCl containing little phloroglucinol. This is heated over a spirit lamp flame and observed for red colouration as indicative of the presence of pentoses.
Test for phlobatannins (HCl test). Two millilitres (2 mL) of the aqueous solution of the extract were added into dilute HCl and observed for red precipitate that was indicative the presence of phlobatannins.
Test for cardiac glycosides. Two millilitres (2 mL) of the aqueous solution of the extract was added into 3 drops of strong solution of lead acetate. This was mixed thoroughly and filtered. The filtrate was shaken with 5 mL of chloroform in a separating funnel. The chloroform layer was evaporated to dryness in a small evaporating dish. The residue was dissolved in a glacial acetic acid containing a trace of ferric chloride; this was transferred to the surface of 2 mL concentrated sulphuric acid in a test tube. The upper layer and interface of the two layers were observed for bluish-green and reddish-brown colouration respectively as indicative of the presence of cardiac glycosides.
Test for steroids (Liebermann-Burchard’s test). The amount of 0.5 g of the extract was dissolved in 10 mL anhydrous chloroform and filtered. The solution was divided into two equal portions for the following tests. The first portion of the solution above was mixed with one ml of acetic anhydride followed by the addition of 1 mL of concentrated sulphuric acid down the side of the test tube to form a layer underneath. The test tube was observed for green colouration as indicative of steroids.
Test for steroids (Salkowski’s test). The second portion of solution above was mixed with concentrated sulphuric acid carefully so that the acid formed a lower layer and the interface was observed for a reddish-brown colour indicative of steroid ring.
Test for flavonoids (Shibita’s reaction test). One gram (1 g) of the water extract was dissolved in methanol (50%, 1-2 mL) by heating, then metal magnesium and 5 - 6 drops of concentrated HCl were added. The solution when red was indicative of flavonols and orange for flavones.
Testforflavonoids (pew’stest). Five millilitres (5 mL) of the aqueous solution of the water extract was mixed with 0.1 g of metallic zinc and 8ml of concentrated sulphuric acid. The mixture was observed for red colour as indicative of flavonols.
Test for anthraquinones (Borntrager’s reaction for free anthraquinones). One gram (1 g) of the powdered seed was placed in a dry test tube and 20 mL of chloroform was added. This was heated in steam bath for 5 min. The extract was filtered while hot and allowed to cool. To the filtrate was added with an equal volume of 10% ammonia solution. This was shaken and the upper aqueous layer was observed for bright pink colouration as indicative of the presence of Anthraquinones. Control test were done by adding 10 mL of 10 % ammonia solution in 5ml chloroform in a test tube.
Elemental analysis. The elemental content was determined using the standard calibration curve method.10 (link),11 (link) Zero point (0.5 g) of air dried sample in an evaporating dish was placed in an oven at 80 ˚C and dried to a constant weight. The sample was placed in a weighing crucible and ashed at 500 ˚C in a hot spot furnance for three hours. The ashed material was prepared for the determination of trace element. A portion of zero point (0.5 g) of the ashed sample was digested by heating for two min with a mixture of 10 mL each of nitric acid (HNO3), HCl and a perechloric acid in a 500 mL flask. The aliquot obtained from this mixture by filtration was mixed with a 10 mL of 2M HNO3 and 30 mL of distilled water in a 100 mL volumetric flask. The volume was made up to zero mark with distilled water. Blank sample and standard solution for the various elements were similarly done. All samples placed in a plastic container and stored in a refrigerator maintained at 4 ˚C prior to analysis. Flame emission spectrometer (Model FGA-330L; Gallenkamp, Weiss, UK) was used to determine sodium (Na) and potassium (K) concentrations. Other elements, magnesium (Mg), calcium (Ca), iron (Fe), lead (Pb), zinc (Zn), manganese (Mn), cadmium (Cd), copper (Cu) and arsenic (As) were determined by atomic absorption spectrometry with (Model SPG No. 1; Unicam, Cambridge, UK) at the appropriate wave-length, temperature and lamp current for each element.12
Auwal M.S., Saka S., Mairiga I.A., Sanda K.A., Shuaibu A, & Ibrahim A. (2014). Preliminary phytochemical and elemental analysis of aqueous and fractionated pod extracts of Acacia nilotica (Thorn mimosa). Veterinary Research Forum : an International Quarterly Journal, 5(2), 95-100.
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Publication 2014
5
Isolation of Ribosomes from E. coli Strains
Cited 18 times
A fresh O/N culture of E. coli JE28 was used to inoculate 1 l. LB with 50 µg/ml kanamycin and grown with aeration at 37°C. At A600 1.0, the culture was slowly cooled to 4°C to produce run-off ribosome and harvested by centrifugation at 4000 rpm for 30 min. The cell-pellet was resuspended in lysis buffer (20 mM Tris–HCl pH 7.6, 10 mM MgCl2, 150 mM KCl, 30 mM NH4Cl and PMSF protease inhibitor 200 µl/l) with lysozyme (0.5 mg/ml) and DNAse I (10 µg/ml) and further lysed using a French Press or sonicator (for smaller cell pellets <2–3 g). The lysate was clarified by centrifuging twice at 18 000 rpm at 4°C, 20 min each. The cleared lysate was divided in half. From one-half 70S ribosome was purified in the conventional method and the affinity-purification method was employed with the other half. In parallel, wild-type ribosome was also purified from the parent strain MG1655 in the conventional way for comparison.
For affinity purification, a HisTrapTMHP column (Ni2+–sepharose pre-packed, 5 ml, GE Healthcare) was connected to an ÄKTA prime chromatography system (GE Healthcare) equilibrated with the lysis buffer. After loading the lysate, the column was washed with 5 mM imidazole until A260 reached the baseline. The tetra-(His)6-tagged ribosomes were then eluted with 150 mM imidazole, pooled immediately and dialyzed 4 × for 10 min in 250 ml lysis buffer to remove the imidazole. Furthermore, the ribosomes were concentrated by centrifugation at 150 000 × g for 2 h at 4°C, resuspended in 1× polymix buffer containing 5 mM ammonium chloride, 95 mM potassium chloride, 0.5 mM calcium chloride, 8 mM putrescine, 1 mM spermidine, 5 mM potassium phosphate and 1 mM dithioerythritol (23 (link)) and shock-frozen in liquid nitrogen for storage or dissolved in the overlay buffer (20 mM Tris–HCl pH 7.6, 60 mM NH4Cl, 5.25 mM Mg acetate, 0.25 mM EDTA and 3 mM 2-mercaptoethanol) for sucrose gradient analysis. As a control, lysate from wild-type E. coli MG1655 was applied to the same column and was treated accordingly.
For purifying JE28 and MG1655 ribosomes in the conventional ultracentrifugation method (24 (link)), the cleared lysate was layered on top of equal volume of 30% w/v sucrose cushion made in a buffer containing 20 mM Tris–HCl pH 7.6, 500 mM NH4Cl, 10.5 mM Mg acetate, 0.5 mM EDTA, and 7 mM 2-mercaptoethanol and centrifuged at 100 000 × g for 16 h at 4°C. This step was repeated twice and in between the ribosome pellet was gently rinsed with the same buffer. Then the pellet was dissolved in 1× polymix buffer for storage or in the overlay buffer for sucrose gradient analysis as in case of the affinity-purified ones.
Ederth J., Mandava C.S., Dasgupta S, & Sanyal S. (2008). A single-step method for purification of active His-tagged ribosomes from a genetically engineered Escherichia coli. Nucleic Acids Research, 37(2), e15.
Publication 2008
2-Mercaptoethanol Acetate Buffers Calcium chloride Cells Centrifugation Chloride, Ammonium Chromatography Chromatography, Affinity Deoxyribonuclease I Dithioerythritol Edetic Acid Escherichia coli Freezing imidazole Kanamycin Magnesium Chloride Muramidase Nitrogen Parent Pellets, Drug Potassium Chloride potassium phosphate Protease Inhibitors Putrescine Ribosomes Sepharose Shock Spermidine Strains Sucrose Tetragonopterus Tromethamine Ultracentrifugation

Most recents protocols related to «Calcium acetate»

1
Soil Amorphous Silica Extraction
Not available on PMC !
The CAL extraction was performed at a solid-to-solution ratio of 1:20 (Schüller, 1969) . Simply, 4 g of the dried soil-ASi mixture was weighed in 100 mL plastic bottles and mixed with 80 mL CAL extraction solution (0.05 M C 6 H 10 CaO 6 , 0.05 M (CH 3 COO) 2 Ca, 96% HAc). The samples were shaken for 2 h, and centrifuged at 10,000xg for 5 min.
Uhuegbue P.O., Stein M., Kalbitz K., & Schaller J. (2024). Silicon effects on soil phosphorus availability: results obtained depend on the method used. Frontiers in Environmental Science, 12.
Publication 2024
2
Bacterial Calcium Carbonate Precipitation Protocol
Assuming that urea can be completely decomposed, 30 mL of a 1 mol/L urea solution contains 1.8018 g of urea, and complete decomposition will yield 3 × 10−2 mol CO32− ; thus, 3 × 10−2 mol Ca2+ or a 5.285 g calcium acetate test sample is required. Twelve test samples with this mass of calcium acetate were weighed and set aside. A 1 mol/L urea solution was prepared in 12 reaction bottles, each set to 30 mL, divided into two groups, A and B. Group A was set as the calcium addition first group by mixing the urea solution with calcium acetate thoroughly, then adding the bacterial solution, and then filtering, drying, and weighing after 48 h of resting. Group B was the later calcium addition group; the urea solution was mixed with the bacterial solution, followed by 24 h of rest, and then the calcium acetate was added to the solution. The solution was allowed to rest for 24 h and was then filtered, dried, and weighed. There were six bacteria-to-calcium ratios: 1/9, 2/9, 3/9, 4/9, 5/9, and 6/9, and the amount of bacterial solution added was 3.3 mL, 6.6 mL, 9.9 mL, 13.2 mL, 16.5 mL, and 19.8 mL, respectively. When the conductivity was measured in this study, the ratio of the bacterial solution to the 1 mol/L urea solution was 1/9, and for the purpose of the control test, the starting bacteria-to-calcium ratio was set to 1/9. Calcium-first and calcium-later addition modes are shown in Figure 1.
Zhao T., Du H, & Shang R. (2024). The Effect of Bacteria-to-Calcium Ratio on Microbial-Induced Carbonate Precipitation (MICP) under Different Sequences of Calcium-Source Introduction. Materials, 17(8), 1881.
Full text: Click here
Publication 2024
3
Calcium Supplementation Impact on RAs
After obtaining the optimal mineralization conditions, calcium ions in the environment were supplemented to analyze the effect of calcium sources on the performance of RAs. The RAs were immersed in 0.1 mol/L calcium acetate solution for 24 h and removed.
Li M., Yi H, & Su Y. (2024). Study on Reducing Water Absorption of Recycled Aggregates (RAs) by Microbial Mineralization. Materials, 17(7), 1612.
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Publication 2024
4
Structural Characterization of Transthyretin Complexes
Crystals of V30M-TTR-4 and V30M-TTR-7 were obtained by a soaking technique.
Parent crystals in an apo form were grown with a crystallization buffer
containing 20 mg/mL V30M-TTR, 29% polyethylene glycol 400 (PEG400),
0.4 M calcium chloride, and 0.1 M sodium acetate pH 5.6. The crystals
were soaked in a buffer containing 1 mM 4 or 7, 30% PEG400, 0.4 M calcium chloride, and 0.1 M sodium acetate pH
5.6 for a week at 293 K. Crystals of V30M-TTR-20 were
obtained by the cocrystallization method with the buffer containing
20 mg/mL V30M-TTR, 1 mM 20, 0.4 M calcium chloride, and
0.1 M sodium acetate pH 5.6. The crystals were cryo-protected using
a cryo-buffer containing 32% PEG400, 0.4 M calcium chloride, 0.1 M
sodium acetate pH 5.6, and 5% DMSO and then directly frozen in liquid
nitrogen until data collection. X-ray diffraction experiments were
conducted on beamline BL-17A at the Photon Factory (Japan), on beamline
NE3A at the Photon Factory Advanced ring, or on beamline X06SA at
the Swiss Light Source (Switzerland). The obtained diffraction data
were processed with XDS software.57 (link) The
crystal complex belonged to the space group P21212 and showed isomorphism with previously obtained
crystals.58 (link),59 (link) Consequently, structure refinements were
directly performed without molecular replacement phasing, utilizing
the apo V30M-TTR (PDB ID 4pwe) as an initial model.58 (link),59 (link) Manual implementation
of crystal structure refinement from diffraction data was carried
out using PHENIX.REFINE and COOT.60 (link),61 (link) The 3D structures
and compound library data were prepared using PRODRG {Schuttelkopf,
2004 #1122}. The final coordinates and structure factors of the V30M-TTR
complexed with 4, 7, and 20 have been deposited in the Protein Data Bank (PDB) under the accession
codes 8WGS, 8WGT, and 8WGU, respectively.
Mizuguchi M., Nakagawa Y., Yokoyama T., Okada T., Fujii K., Takahashi K., Luan N.N., Nabeshima Y., Kanamitsu K., Nakagawa S., Yamakawa S., Ueda M., Ando Y, & Toyooka N. (2024). Development of Benziodarone Analogues with Enhanced Potency for Selective Binding to Transthyretin in Human Plasma. Journal of Medicinal Chemistry, 67(9), 6987-7005.
Publication 2024
5
Crystallization and Structure Determination of YjfP and YqiA
Not available on PMC !
YjfP and YqiA were crystallized using sitting drop vapour diffusion and screened for crystallization against several broad screens. 0.2 µL of protein solutions at 10 mg/mL were mixed with 0.2 µL of mother liquor. YjfP appeared as needle-like crystals in several different conditions, however the final structure of YjfP was determined from cubic crystals grown in 0.1 M ammonium sulfate, 35 % w/v PEG 8,000, 0.1 M sodium acetate pH 5.0. A YjfP crystal from this condition was soaked in mother liquor supplemented with 25 % v/v ethylene glycol for one minute before freezing in liquid nitrogen for analysis at the Australian Synchrotron. YqiA crystallized in several conditions that mostly contained either calcium chloride or calcium acetate, a crystal grown in 0.3 M calcium chloride hexahydrate, 10 % w/v PEG 6,000, 0.1 Tris pH 8.0 led to successful structure determination of YqiA. This crystal was soaked in mother liquor supplemented with 25 % v/v glycerol for one minute, before being frozen in liquid nitrogen.
Uddin M.J., Randall G., Zhu J., Upadhyay T., Eijk L.v., Stege P.B., Masson F.M., Viveen M.C., Bogyo M., Fellner M., Zoete M.R.d., Johannessen M., & Lentz C.S. (2024). Activity-Based Protein Profiling Identifies Klebsiella pneumoniae Serine Hydrolases with Potential Roles in Host-Pathogen Interactions.
Publication 2024

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2
Sodium chloride (nacl) by Merck Group
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NaCl is a chemical compound commonly known as sodium chloride. It is a white, crystalline solid that is widely used in various industries, including pharmaceutical and laboratory settings. NaCl's core function is to serve as a basic, inorganic salt that can be used for a variety of applications in the lab environment.
3
Hydrochloric acid (hcl) by Merck Group
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4
Methanol by Merck Group
Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan
Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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Ammonium acetate by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, Spain, India, France, China, Australia, Switzerland, Macao, Sao Tome and Principe, Canada, Ireland, Czechia, Belgium, Netherlands, Poland, Japan, Austria, Hungary, Finland, Mexico, Sweden, Romania
Ammonium acetate is a chemical compound with the formula CH3COONH4. It is a colorless, crystalline solid that is soluble in water and alcohol. Ammonium acetate is commonly used in various laboratory applications, such as pH adjustment, buffer preparation, and as a mobile phase component in chromatography.
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Acetonitrile by Merck Group
Sourced in Germany, United States, Italy, India, China, United Kingdom, France, Poland, Spain, Switzerland, Australia, Canada, Brazil, Sao Tome and Principe, Ireland, Belgium, Macao, Japan, Singapore, Mexico, Austria, Czechia, Bulgaria, Hungary, Egypt, Denmark, Chile, Malaysia, Israel, Croatia, Portugal, New Zealand, Romania, Norway, Sweden, Indonesia
Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.
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Phorbol myristate acetate (pma) by Merck Group
Sourced in United States, Germany, United Kingdom, France, Italy, China, Canada, Switzerland, Sao Tome and Principe, Macao, Poland, Japan, Australia, Belgium, Hungary, Netherlands, India, Denmark, Chile
The PMA is a versatile laboratory equipment designed for precision measurement and analysis. It functions as a sensitive pressure transducer, accurately measuring and monitoring pressure levels in various applications. The PMA provides reliable and consistent data for research and testing purposes.
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Sodium hydroxide (naoh) by Merck Group
Sourced in Germany, United States, India, United Kingdom, Italy, China, Spain, France, Australia, Canada, Poland, Switzerland, Singapore, Belgium, Sao Tome and Principe, Ireland, Sweden, Brazil, Israel, Mexico, Macao, Chile, Japan, Hungary, Malaysia, Denmark, Portugal, Indonesia, Netherlands, Czechia, Finland, Austria, Romania, Pakistan, Cameroon, Egypt, Greece, Bulgaria, Norway, Colombia, New Zealand, Lithuania
Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.
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Sodium acetate by Merck Group
Sourced in United States, Germany, United Kingdom, Spain, India, Italy, France, China, Poland, Canada, Australia, Ireland, Hungary, Mexico, Chile, Switzerland, Brazil, Macao, Netherlands, Belgium, New Zealand, Portugal, Czechia, Sweden, Sao Tome and Principe
Sodium acetate is a chemical compound with the formula CH3COONa. It is a common salt that is widely used in various laboratory and industrial applications. Sodium acetate functions as a buffer solution, helping to maintain a specific pH level in chemical reactions and processes.
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Acetic acid by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, India, China, France, Spain, Switzerland, Poland, Sao Tome and Principe, Australia, Canada, Ireland, Czechia, Brazil, Sweden, Belgium, Japan, Hungary, Mexico, Malaysia, Macao, Portugal, Netherlands, Finland, Romania, Thailand, Argentina, Singapore, Egypt, Austria, New Zealand, Bangladesh
Acetic acid is a colorless, vinegar-like liquid chemical compound. It is a commonly used laboratory reagent with the molecular formula CH3COOH. Acetic acid serves as a solvent, a pH adjuster, and a reactant in various chemical processes.

More about "Calcium acetate"

Calcium Acetate: A Versatile Compound with Diverse Applications

Calcium acetate, also known as E260 or acetic acid calcium salt, is a chemical compound with a wide range of uses in research, industry, and everyday life.
This versatile substance is essential for the production of pharmaceuticals, food additives, and various industrial materials.
Researchers and scientists can leverage the power of PubCompare.ai, an AI-driven platform, to optimize their calcium acetate studies.
This innovative tool provides access to a wealth of reliable protocols from literature, pre-prints, and patents, helping users identify the most effective methods and products for their specific needs.
With automated comparisons, PubCompare.ai enables researchers to enhance the reproducibility and accuracy of their calcium acetate experiments.
By streamlining the research process, the platform empowers scientists to take their calcium acetate studies to new heights, unlocking valuable insights and breakthroughs.
Calcium acetate's versatility extends beyond its primary applications.
Related compounds, such as calcium chloride (CaCl2), sodium chloride (NaCl), and hydrochloric acid (HCl), can also play crucial roles in various research and industrial processes.
Similarly, solvents like methanol (CH3OH), ammonium acetate (CH3COONH4), and acetonitrile (CH3CN) are often utilized in conjunction with calcium acetate.
Other relevant substances, such as PMA (Polymethacrylic Acid), sodium hydroxide (NaOH), sodium acetate (CH3COONa), and acetic acid (CH3COOH), may also be integrated into calcium acetate-based studies and applications.
By exploring the diverse connections and synergies between calcium acetate and these related compounds, researchers can uncover new possibilities and optimize their experimental approaches, leading to groundbreaking discoveries and advancements in their respective fields.
OtherTerms: Calcium acetate, E260, acetic acid calcium salt, CaCl2, NaCl, HCl, CH3OH, CH3COONH4, CH3CN, PMA, NaOH, CH3COONa, CH3COOH