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Campesterol

Campesterol is a phytosterol, a type of plant-derived sterol, that plays a crucial role in plant cell membrane structure and function.
It is commonly found in various plant sources, including soybeans, corn, and wheat.
Campesterol has been the subject of extensive research due to its potential health benefits, such as its ability to modulate cholesterol levels and its anti-inflammatory properties.
The study of campesterol is important for understanding plant physiology as well as developing potential therapeutic applications in areas like cardiovascular health and disease prevention.
PubCompare.ai can help streamline your campesterol research by providing access to the best protocols and products, along with AI-driven comparisons to ensure reproducibility and accuracy in your findings.

Most cited protocols related to «Campesterol»

1
GC-MS Quantification of Phenolic Acids and Sterols
Cited 4 times
The absolute quantification of ρ-coumaric acid, isoferulic acid, caffeic acid, sinapic acid, campesterol, and β-sitosterol was accomplished using calibration curves of each standard compound. Nine concentration solutions were prepared to achieve a concentration of 0.625 to 200 μg/mL by dissolving precisely weighed amounts of ρ-coumaric acid, isoferulic acid, caffeic acid, campesterol, and β-sitosterol in ethanol and sinapic acid in methanol. Two hundred microliters of each standard solution was transferred into GC vials and dried with nitrogen gas for 5 min. For derivatization, 30 μL of 20,000 μg/mL methoxylamine hydrochloride in pyridine, 50 μL of BSTFA (N,O-bis (trimethylsilyl) trifluoroacetamide; Alfa Aesar, Ward Hill, MA, USA) containing 1% trimethylchlorosilane, and 10 μL of 3000 μg/mL myristic acid-d27 (Tokyo Chemical Industry Co., Ltd.) in pyridine used as an internal standard (IS), were added to each standard compound of various concentrations. The samples were incubated in a 65°C water bath for 60 min, and the derivatized standard solutions of each compound were injected into the GC-MS in triplicate. The standard calibration curves were achieved by plotting concentration against intensity ratio between compounds and the internal standard. The means of the slopes (S) and standard deviation of the intercepts (σ) were calculated. The limit of detection (LOD) and the limit of quantification (LOQ) were calculated by following equations.
Kim J.Y., Kim H.Y., Jeon J.Y., Kim D.M., Zhou Y., Lee J.S., Lee H, & Choi H.K. (2017). Effects of coronatine elicitation on growth and metabolic profiles of Lemna paucicostata culture. PLoS ONE, 12(11), e0187622.
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Publication 2017
Bath caffeic acid campesterol Coumaric Acids Ethanol Gas Chromatography-Mass Spectrometry isoferulic acid Methanol methoxyamine hydrochloride ML-3000 Myristic Acid N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide Nitrogen pyridine sinapinic acid sitosterol trifluoroacetamide trimethylchlorosilane
2
Quantification of Sterols in Human Serum
Cited 3 times
Peaks of desmosterol (purity ≥84%), 7-dehydrocholesterol (purity ≥95), lathosterol (purity >99%), campesterol (purity ∼65%) and b-sitosterol (purity TraceCERT® grade) were identified by comparison with corresponding HPLC grade analytical standards (Supelco, Bellefonte, PA, USA). Deuterated internal standard (IS) d6-cholesterol (HPLC grade) purchased from Sigma-Aldrich (St. Louis, MO, USA) was used. Cholesterol standard of ≥99% purity was obtained from Sigma Aldrich (St. Louis, MO, USA). KOH was purchased from POCH (Center Valley, PA, USA), and ethanol, methanol, n-hexane and acetonitrile (HPLC grade) from Fisher (Pittsburgh, PA, USA). Bovine serum albumin (BSA) was obtained from Sigma-Aldrich (St. Louis, MO, USA).
Commercial cholesterol HDL precipitating reagent produced by BioSystems (Costa Brava, Barcelona, Spain) contained phosphotungstate (0.4 mmol/L) and magnesium chloride (20 mmol/L).
Human serum samples were obtained from healthy volunteers after the general medical examination with permission from local Ethical committee and following the guidelines defined by the Declaration of Helsinki. Samples were used for method development and clinical verification. All participants signed an informed consent form before the enrolment.
HPLC method development and sample analyses were done using C-18 Porochell 120-EC column (150×4.6 mm×2.7 μm) (Agilent Technologies, USA).
Vladimirov S., Gojković T., Zeljković A., Jelić-Ivanović Z, & Spasojević-Kalimanovska V. (2020). Determination of non-cholesterol sterols in serum and HDL fraction by LC/MS-MS: Significance of matrix-related interferences. Journal of Medical Biochemistry, 39(3), 299-308.
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Publication 2020
7-dehydrocholesterol acetonitrile campesterol Cholesterol Desmosterol Ethanol Healthy Volunteers High-Performance Liquid Chromatographies High Density Lipoprotein Cholesterol Homo sapiens lathosterol Magnesium Chloride Methanol n-hexane Ribs Serum Serum Albumin, Bovine sitosterol
3
Sterol Profiling by LC-MS/MS
Cited 3 times
Compounds were separated using a Zorbax SB-C18 reversed-phase analytical column (100 mm × 3.0 mm i.d., 5 μm particle) fitted with a guard column Zorbax SB-C18, both operated at 40°C. Sterols were separated under isocratic conditions using a mobile phase consisting of 10:90 (v/v) methanol and acetonitrile. The flow rate was 1 mL/min and the injection volume was 5 μL. Mass spectrometry analysis was performed on an Agilent Ion Trap 1100 VL mass spectrometer with atmospheric pressure chemical ionization (APCI) interface. The instrument was operated in positive ion mode. Operating conditions were optimized in order to achieve maximum sensitivity values: gas temperature (nitrogen) 325°C at a flow rate of 7 L/min, nebulizer pressure 60 psi and capillary voltage -4000 V.
The identification of sterols was performed by comparing the retention times and mass spectra with those of standards in the same chromatographic conditions. To avoid or limit the interference from background, the multiple reactions monitoring analysis mode was used instead of single ion monitoring (e.g., MS/MS instead of MS). Linearity of calibration curves was very good (R2 > 0.998), with detection limits in the range of 69 to 3312 ng/mL for ergosterol, 62 to 2952 ng/mL for brassicasterol, 59 to 2808 ng/mL for campesterol, 136 to 6528 ng/mL for stigmasterol, and 132 to 6336 ng/mL for β-sitosterol. The results are expressed as μg per mL of extract (μg/mL).
The software ChemStation (vA09.03) and DataAnalysis (v5.3) from Agilent, United States were used for the acquisition and analysis of chromatographic data (Vlase et al., 2013 (link)).
Toiu A., Mocan A., Vlase L., Pârvu A.E., Vodnar D.C., Gheldiu A.M., Moldovan C, & Oniga I. (2018). Phytochemical Composition, Antioxidant, Antimicrobial and in Vivo Anti-inflammatory Activity of Traditionally Used Romanian Ajuga laxmannii (Murray) Benth. (“Nobleman’s Beard” – Barba Împăratului). Frontiers in Pharmacology, 9, 7.
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Publication 2018
acetonitrile Atmospheric Pressure brassicasterol campesterol Capillaries Chromatography Ergosterol Hypersensitivity Mass Spectrometry Methanol Nebulizers Nitrogen Phytosterols Pressure Retention (Psychology) sitosterol Stigmasterol Tandem Mass Spectrometry
4
Phytosterol Extraction and GC-MS Analysis
Cited 3 times
Phytosterol extraction was modified from Itkin et al. (54 (link)). Fifty milligrams of frozen, powdered plant material was extracted in 2 mL choloform/MeOH (2:1 vol/vol) at 75 °C for 1 h. After 1 h at room temperature, the solvent was evaporated in a vacuum concentrator. The dried residue was saponified in 500 µL 6% (wt/vol) KOH in MeOH for 1 h at 90 °C. After samples cooled to room temperature, 500 µL H2O and 500 µL n-hexane were added, shaken for 30 s, and centrifuged briefly to separate the phases. The hexane phase was transferred to a clean 1,500-µL Eppendorf tube and dried in a vacuum concentrator. The remaining aqueous phase was extracted twice more with 500 µL n-hexane, which was added to the dried first extract and dried. Dried samples were resuspended in 50 μL N-methyl-N-trimethylsilyl trifluoroacetamide (Sigma), transferred to 2-mL glass vials with a 100-μL glass insert containing 0.01 µg 5-α-cholestane (Sigma), and incubated at room temperature at least 15 min before analysis.
GC-MS analysis was performed on a Shimadzu GCMS-TQ8040 with a DB-1 (30 m × 0.25 mm, 0.25-µm film thickness; Agilent) or HP-5 (30 m × 0.32 mm, 0.25-µm film thickness; SGE Analytical Science) capillary column following the method described by Seki et al. (27 (link)) with modifications. Injection temperature was set at 250 °C with a split ratio of 15. Temperature program was as follows: 80 °C for 1 min followed by temperature increase to 300 °C at a rate of 20 °C/min and hold at 300 °C for 10–28 min. Cholesterol, campesterol, stigmasterol, and β-sitosterol were identified based on authentic standards purchase from Tama Biochemical Co. Authentic standards for 24-methylenecholesterol and [26-13C]-labeled 24-methyldesmosterol were prepared with their structural confirmation in the previous study (55 , 56 ). Quantification of sterols was based on a standard calibration curve of authentic standards.
Knoch E., Sugawara S., Mori T., Poulsen C., Fukushima A., Harholt J., Fujimoto Y., Umemoto N, & Saito K. (2018). Third DWF1 paralog in Solanaceae, sterol Δ24-isomerase, branches withanolide biosynthesis from the general phytosterol pathway. Proceedings of the National Academy of Sciences of the United States of America, 115(34), E8096-E8103.
Publication 2018
24-methyldesmosterol 24-methylenecholesterol campesterol Capillaries Cholestanes Cholesterol Fever Freezing Gas Chromatography-Mass Spectrometry n-hexane Phytosterols Plants sitosterol Solvents Stigmasterol trifluoroacetamide Vacuum
5
Quantification of Sterols and Oxysterols in PKAN
Cited 3 times

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Publication 2011
24-hydroxycholesterol 27-hydroxycholesterol Argon ARID1A protein, human campesterol Cells Cholesterol Cultured Cells Cyclohexane Edetic Acid Ethanol Ethers Gas Chromatography-Mass Spectrometry Hallervorden-Spatz Syndrome Helium hexamethyldisilazane Hydrolysis Hydroxycholesterols Hydroxytoluene, Butylated Ions lathosterol Nitrogen Oxysterols Patients Phocidae Phytosterols Plasma potassium hydroxide Proteins pyridine sitosterol Sodium Chloride Solvents Sterols Teflon trimethylchlorosilane

Most recents protocols related to «Campesterol»

1
Colocalization Analysis of Campesterol and Cholelithiasis
We also performed a colocalization analysis between the hit (campesterol) and cholelithiasis with coloc R package (https://cran.r-project.org/web/packages/coloc/vignettes/a01_intro.html). The SNP would be considered as causal to one trait when the default prior probability was lower than 1 × 10−4. If the posterior probability of one SNP being shared between the two traits in one region was greater than 0.95, we considered it as a signal of colocalization. The Locus zoom plots were generated using the online website tool (http://locuszoom.org/) based on the GRCh17 reference genome.
Mi J., Jiang Q., Qi Z., Liu Z., Bai X., Zheng X., Wu J., Fang Y., Yang A, & Chen H. (2024). Plasma campesterol and ABCG5/ABCG8 gene loci on the risk of cholelithiasis and cholecystitis: evidence from Mendelian randomization and colocalization analyses. Human Genomics, 18, 19.
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Publication 2024
2
Mendelian Randomization for Cholesterol Metabolism
The Wald ratio was used to estimate the effect size when there was only one SNP available as an instrumental variable. The inverse-variance weighted (IVW) was used as the main method for causal estimation when there were multiple SNPs used as proxies. In addition, we used MR-Egger and weighted median methods for sensitivity analyses. The MR-Egger method was used to identify potential pleiotropy effects based on the p-value for the intercept. The weighted median method is utilized to strengthen causal estimates. We also performed bi-directional MR using cholelithiasis as the exposures and campesterol from CLSA cohort as the outcome. We selected IVs using the conventional genome-wide significance (p < 5 × 10−8) with linkage disequilibrium (LD) threshold of R2 < 0.001 within ± 5000 kilobase (kb) distance for the extraction of LD-independent SNPs from the corresponding GWAS used as instrumental variables.
Mi J., Jiang Q., Qi Z., Liu Z., Bai X., Zheng X., Wu J., Fang Y., Yang A, & Chen H. (2024). Plasma campesterol and ABCG5/ABCG8 gene loci on the risk of cholelithiasis and cholecystitis: evidence from Mendelian randomization and colocalization analyses. Human Genomics, 18, 19.
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Publication 2024
3
Phytosterol profiling of oil samples
Not available on PMC !
Oil samples were processed and analyzed by GC-FID to obtain their phytosterol profile as previously described 21 (link) . Standards of single sterols (β-sitosterol ≥95%, campesterol ~65% and stigmasterol ~95%) (Sigma-Aldrich, Schnelldorf, Germany) were used for identification and quantification. Analyses were carried out in triplicate and results are reported as mean value ± standard deviation.
Rincón‐Cervera M.Á., Romero N., Jiménez P., Palma D.S., Farías C., Cisternas C., Guzmán C., Hevia L., & Valenzuela R. (2024). Effect of refining and pressing temperature on chemical attributes of canola oil produced in Chile. Revista chilena de nutrición, 51(1), 7-14.
Publication 2024
4
Fatty Acid Composition Analysis
Not available on PMC !
Glacial acetic acid, potassium iodine, sodium thiosulfate, sodium hydroxide, soluble starch, analytical-grade chlorophorm, pyridine, isoctone, THF, and methanol were purchased from Merck (Darmstadt, Germany). Methanolic boron trifluoride (BF 3 ) (10%), Fatty Acid Methyl Esters (Standard FAME 37), N, and O-bis(trimethylsilyl)trifluoroacetamide with trimethylchlorosilane (BSFTA + TMCS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The external phytosterols standards (cholesterol, stigmasterol, campesterol, brassicasterol, and β-sitosterol) were purchased from Dr. Ehrenstorfer (Augsburg, Germany).
Ordúz-Díaz L.L., Lozano-Garzón K., Quintero-Mendoza W., Díaz R., Cardona J., Carrillo M.P., Guerrero D.C., & Hernández M. (2024). Effect of Fermentation and Extraction Techniques on the Physicochemical Composition of Copoazú Butter (Theobroma grandiflorum) as an Ingredient for the Cosmetic Industry. Cosmetics, 11(3), 77-77.
Publication 2024
5
Lipid Profiling of Natural Standards
Not available on PMC !
FAME standard mixture (C4-C24) and isooctane were purchased from Supelco (UK). Protease (from Bacillus amyloliquefaciens), cellulase (from Trichoderma reesei), Folin-Ciocalteu reagent, 5a-cholestan-3b-ol ($95%), sodium methoxide solution (0.5 M, ACS grade), b-sitosterol ($95%), cholesterol ($99%), squalene ($98%), campesterol (∼65%), stigmasterol (∼95%), a-tocopherol ($96%, HPLC grade), g-tocopherol ($96%, HPLC grade), and d-tocopherol ($90%) standards were purchased from Sigma-Aldrich (UK). The Tri-sil HTP reagent was purchased from Thermo Scientic (UK).
Zhang G., Li Z., & Fu M. (2024). Comparison of quality and oxidative stability of pumpkin seed oil (Cucurbita maxima) between conventional and enzymatic extraction methods. Sustainable Food Technology.
Publication 2024

Top products related to «Campesterol»

1
Campesterol by Merck Group
Sourced in United States, Germany, China, Australia, Italy, France
Campesterol is a plant sterol compound found in various plant sources. It is a natural component that can be isolated and used as a reference standard or analytical tool in laboratory settings.
2
β sitosterol by Merck Group
Sourced in United States, Germany, China, Australia, Italy, France, Japan
β-sitosterol is a phytosterol compound commonly found in plants. It is a naturally occurring sterol that is structurally similar to cholesterol. β-sitosterol is often used as a reference standard in analytical applications.
3
Stigmasterol by Merck Group
Sourced in United States, Germany, China, Australia, Spain, United Kingdom, Italy, France
Stigmasterol is a plant-derived sterol compound commonly used as a reference standard and analytical tool in laboratory settings. It serves as a key component in various analytical techniques, such as chromatography and spectroscopy, to identify and quantify similar sterol compounds in samples. Stigmasterol's core function is to provide a reliable and well-characterized reference point for the analysis and identification of other sterols in research and testing applications.
4
Cholesterol by Merck Group
Sourced in United States, Germany, United Kingdom, India, Japan, Sao Tome and Principe, China, France, Spain, Canada, Switzerland, Italy, Australia, Israel, Brazil, Belgium, Poland, Hungary, Macao
Cholesterol is a lab equipment product that measures the concentration of cholesterol in a given sample. It provides quantitative analysis of total cholesterol, HDL cholesterol, and LDL cholesterol levels.
5
5α cholestane by Merck Group
Sourced in United States, Germany, China, Poland
5α-cholestane is a laboratory standard compound used as a reference material for analytical procedures. It is a naturally occurring steroid hydrocarbon found in various biological samples. 5α-cholestane serves as a marker compound for identification and quantification purposes in analytical techniques such as gas chromatography and mass spectrometry.
6
Brassicasterol by Merck Group
Sourced in United States, Germany, Australia
Brassicasterol is a phytosterol compound found in the oils of certain plants, such as brassica species. It is commonly used as a reference standard in analytical laboratory equipment for the identification and quantification of plant-derived sterols. The core function of Brassicasterol is to serve as a reference material for analytical purposes, providing a known standard for comparison and validation of test results.
7
Sitosterol by Merck Group
Sourced in United States, Germany
Sitosterol is a phytosterol compound derived from plant sources. It is used as a raw material in the manufacturing of various pharmaceutical and biochemical products. Sitosterol serves as a core ingredient in the production of steroids and other related compounds. Its primary function is to provide a natural source of sterols for further chemical processing and formulation development.
8
Ergosterol by Merck Group
Sourced in United States, Germany, China, Spain, Brazil, Sao Tome and Principe, Poland
Ergosterol is a lipid molecule found in the cell membranes of fungi. It is a key component of the fungal cell membrane and plays a critical role in maintaining the structural integrity and permeability of the membrane. Ergosterol is often used as a biomarker for the presence and quantification of fungi in various applications, such as environmental monitoring and pharmaceutical quality control.
9
Squalene by Merck Group
Sourced in United States, Germany, United Kingdom, France, China, Spain, Japan, Italy
Squalene is a natural organic compound that is widely used in laboratory settings as a component in various types of lab equipment. It functions as a key ingredient in the formulation of various substances used in scientific research and testing. Squalene is derived from natural sources and is a commonly used material in the manufacture of specialized laboratory equipment and consumables.
10
Methanol by Merck Group
Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan
Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.

More about "Campesterol"

Campesterol is a phytosterol, a type of plant-derived sterol that plays a crucial role in plant cell membrane structure and function.
It is commonly found in various plant sources, including soybeans, corn, and wheat.
Campesterol is closely related to other plant sterols like β-sitosterol, stigmasterol, and brassicasterol, all of which share similar chemical structures and physiological functions.
Campesterol has been the subject of extensive research due to its potential health benefits, such as its ability to modulate cholesterol levels and its anti-inflammatory properties.
It is often compared to cholesterol, a structurally similar animal sterol, and 5α-cholestane, another plant-derived sterol.
The study of campesterol is important for understanding plant physiology as well as developing potential therapeutic applications in areas like cardiovascular health and disease prevention.
PubCompare.ai can help streamline your campesterol research by providing access to the best protocols and products, along with AI-driven comparisons to ensure reproducibility and accuracy in your findings.
The platform also offers insights into related plant sterols like sitosterol and ergosterol, as well as squalene, a precursor to campesterol and other sterols.
With PubCompare.ai, you can effortlessly locate the optimal methods from literature, pre-prints, and patents, while our AI-driven comparisons ensure the quality and reliability of your campesterol research.