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Camptothecin

Camptothecin is a plant-derived alkaloid with potent antitumor activity.
It inhibits the enzyme topoisomerase I, leading to the accumulation of DNA single-strand breaks and cell death.
Camptothecin and its derivatives have shown promising results in the treatment of various cancers, including lung, ovarian, and colorectal cancers.
Researchers can utilize the PubCompare.ai platform to efficiently locate the best protocols from literature, preprints, and patents, using AI-driven comparisons to identify the optimal methods and products for their Camptothecin research needs.
This innovative solution can help streamline Camptothecin studies and enhance the reproducibility and accuracy of thier findings.

Most cited protocols related to «Camptothecin»

1
Apoptosis Evaluation by EB/AO Staining
Cited 60 times
Four ml of 5 × 105 cells/well were seeded in a 6-well plate the night before the treatment. Cells were treated with camptothecin at a final concentration of 6 μM for 4 hr in the 37°C incubator before the cells were subjected to EB/AO staining. Then, 1 ml of cell suspension was used for conventional EB/AO staining, and 100 μl of cell suspension was transferred to a 96-well plate for modified EB/AO staining.
Ribble D., Goldstein N.B., Norris D.A, & Shellman Y.G. (2005). A simple technique for quantifying apoptosis in 96-well plates. BMC Biotechnology, 5, 12.
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Publication 2005
Camptothecin Cells
2
CtIP-Mediated DNA Damage Response
Cited 53 times
HeLa, HCC1937, U2OS and U2OS-derived cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% foetal bovine serum (FBS) and standard antibiotics. Data for survival curves were generated by colony formation assays. In brief, U2OS cells were transfected with siRNA (see Supplementary Methods) and treated with DNA-damage-inducing drugs. After 1 h, the drug was removed and cells were left for 10-14 days at 37°C to allow colonies to form. Colonies were stained with 0.5% crystal violet/20% ethanol and counted. Where indicated, cells were pre-incubated with aphidicolin (10 μM) for 90 min, then treated with the specified drug and aphidicolin for 1 h. A U2OS-derived cell line stably expressing GFP-ATR was described previously7 (link). The siRNA-resistant silent wild-type GFP-CtIP construct was generated by sub-cloning the CtIP cDNA into the pEGFP-C1 expression plasmid (BD Biosciences Clontech) and changing three nucleotides in the CtIP-1 siRNA targeting region by using a QuikChange site-directed mutagenesis kit (Stratagene, Inc.) as previously described16 (link). The plasmid expressing the CtIP mutant lacking the C-terminus (1-789) was generated by changing residues 790 and 791 in the wild-type GFP-CtIP construct to two stop-codons. For generation of cell lines stably expressing siRNA-resistant GFP-tagged wild-type and mutant CtIP, U2OS cells were transfected with the appropriate constructs and, following antibiotic selection, resistant clones were tested for expression and nuclear localization of the transgene-product by immunofluorescence microscopy. To detect ssDNA by microscopy, cells were cultivated for 24 h in medium supplemented with 10 μM BrdU prior to camptothecin treatment and, after fixation, immunostained with an anti-BrdU antibody (see Methods) without any preceding DNA denaturation or nuclease treatment48 (link). Laser micro-irradiation was performed as described previously30 (link),31 (link). Recombinant FLAG-GST-CtIP-6H was isolated from baculovirus-infected Sf9 cells as described previously49 (link). Recombinant MRE11-RAD50 (MR) and MRE11-RAD50-NBS1 (MRN) complex were kind gifts from T. Paull.
Full Methods and any associated references are available in the online version of the paper at www.nature.com/nature.
Sartori A.A., Lukas C., Coates J., Mistrik M., Fu S., Bartek J., Baer R., Lukas J, & Jackson S.P. (2007). Human CtIP promotes DNA end resection. Nature, 450(7169), 509-514.
Publication 2007
Antibiotics Antibodies, Anti-Idiotypic Aphidicolin Baculoviridae Biological Assay Bromodeoxyuridine Camptothecin Cell Lines Cells Clone Cells Codon, Terminator DNA, Complementary DNA, Single-Stranded DNA Damage DNA Denaturation Eagle Ethanol Fetal Bovine Serum Gifts HeLa Cells Immunofluorescence Microscopy Microscopy Mutagenesis, Site-Directed Nucleotides Pharmaceutical Preparations Plasmids Rad50 protein, human Radiotherapy RBBP8 protein, human RNA, Small Interfering Sf9 Cells Transgenes Violet, Gentian
3
Induction of Mitosis Arrest and Apoptosis
Cited 28 times
The inhibition of mitosis and the induction of apoptosis in KG1a and MV4–11 cells were induced respectively by exposure to camptothecin (Sigma-Aldrich, Saint-Quentin Fallavier, France), a cytotoxic quinoline alkaloid which inhibits the DNA enzyme topoisomerase I [10] (link), [11] (link) and by AZD8055 (AstraZeneca Cancer & Infection Research Area, Alderley Park, UK) [12] (link), a selective inhibitor of mTOR kinase, respectively. Cells were seeded at 2×105 cells/mL (5% CO2 incubator at 37°C). KG1a cells were cultured for 6h with camptothecin at a final concentration of 1 µM and MV4–11 cells were cultured for 24 h with AZD8055 at a final concentration of 10 nM and 100 nM. The stock solutions were diluted to ensure a final concentration of <0.03% for DMSO (Sigma-Aldrich). Control cultures were treated with an equivalent volume of DMSO in MEM alpha medium which did not induce apoptosis.
Quiescence was induced in KG1a cells by contact with BM MSCs [13] (link). Adherent culture-amplified MSCs were used at passage 2 (P2). KG1a cells were co-cultured on P2-MSCs for 72 h (37°C in 95% humidified air and 5% CO2) at a starting concentration of 1.5×104/cm2.
The accumulation of KG1a cells in the M phase was induced by exposure to colcemid (KaryoMax Colcemid, Life Technologies), used for arresting the dividing cell at metaphase of mitosis. Cells were cultured 30 min and 1 h with colcemid at a final concentration of 0.1 µg/mL.
Lymphocytes stimulation was induced by exposure to phytohemagglutinin (PHA) (Remel™, Oxoid™, Haarlem, The Netherlands), which is used to stimulate mitotic division of lymphocytes. Whole blood cells were cultured 72 h with PHA at a final concentration of 170 µg/mL according to the manufacturer’s recommandations.
All experiments were performed in triplicate.
Vignon C., Debeissat C., Georget M.T., Bouscary D., Gyan E., Rosset P, & Herault O. (2013). Flow Cytometric Quantification of All Phases of the Cell Cycle and Apoptosis in a Two-Color Fluorescence Plot. PLoS ONE, 8(7), e68425.
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Publication 2013
Apoptosis AZD8055 Blood Cells Camptothecin Cells Colcemide Division Phase, Cell Enzymes Infection Lymphocyte Lymphocyte Activation Malignant Neoplasms Metaphase Mitosis MTOR Inhibitors Phytohemagglutinins Plant Alkaloids Psychological Inhibition quinoline Sulfoxide, Dimethyl TOP1 protein, human
4
Apoptosis Induction in Cell Lines
Cited 18 times
HCT116 cells were purchased from ATCC (Manassas, VA) and AAV-293 cells from Stratagene (Cedar Creek, TX). Bax−/− HCT116 cells were provided by Bert Vogelstein (Zhang et al 2000 (link)). WT, Bax−/−, Bak−/− and Bax−/−Bak−/− DKO MEF cells were provided by Stanley Korsmeyer (Wei et al 2001 ). Cells were cultured as described (Cleland et al 2011 (link)). 5-FU (fluorouracil), camptothecin, sulindac sulfate, cisplatin, TRAIL, indomethacin and staurosporine were purchased from Sigma (St. Louis, MO) and dissolved in DMSO for stock preparation. ABT-737 was purchased from Selleck Chemicals LLC (Houston, Texas).
Wang C, & Youle R.J. (2011). Predominant requirement of Bax for apoptosis in HCT116 cells is determined by Mcl-1’s inhibitory effect on Bak. Oncogene, 31(26), 3177-3189.
Publication 2011
ABT-737 Camptothecin Cells Cisplatin Fluorouracil HCT116 Cells Indomethacin Staurosporine Sulfates, Inorganic Sulfoxide, Dimethyl Sulindac TNFSF10 protein, human
5
Investigating Transcriptional Regulation Dynamics
Cited 11 times
We grew cells overnight on 60mm plates to 70-80% confluency and then treated them with 100μM of 5,6-Dichlorobenzimidazole 1-β–D-ribofuranoside (DRB) (Sigma) in culture medium for 3 hours. The cells were washed twice with PBS to remove the DRB and incubated in fresh medium for various time periods. Following the incubation period, cells were directly lysed and total RNA was isolated using a High Pure RNA isolation kit (Roche) as per the manufacturer's instructions. Actinomycin D (Sigma) or α-amanitin (Sigma) whenever used, were added at a concentration of 1μg/ml and 2μg/ml respectively. Camptothecin (CPT) (Sigma) was used at 15μM concentration and was added 15 minutes before completion of the DRB treatment. The PBS that was used for subsequent washings also contained 15μM CPT and then fresh medium containing 15μM CPT was added to the cells for various time periods.
Singh J, & Padgett R.A. (2009). Rates of in situ transcription and splicing in large human genes. Nature structural & molecular biology, 16(11), 1128-1133.
Publication 2009
Amanitins Camptothecin Cells Dactinomycin isolation

Most recents protocols related to «Camptothecin»

1
Synthesis of Camptothecin Prodrugs
Not available on PMC !

Example 18

[Figure (not displayed)]

a.1) Synthesis of camptothecin prodrug 1: Camptothecin prodrug (1) will be produced by the direct coupling of the compound with PAzPC (fatty acid modified oxidized lipid 16:0-9:0 COOH PC) in presence of DCC/DMAP mediated coupling protocol.

a.2) Synthesis of camptothecin prodrug 2: Camptothecin will be activated with bis(4-nitrophenyl) carbonate followed by reacting with monoboc-ethylendimine to produce 6. In a typical experimental procedure, under a N2 atmosphere, a mixture of camptothecin, bis(4-nitrophenyl) carbonate and DMAP in dry CH2Cl2 will be stirred for 7 h. The reaction mixture will be diluted with CH2Cl2 and washed with H2O. The organic layer will be dried (Na2SO4) and concentrated. Flash chromatography (EtOAc-hexane) will be used to yield the activated fumagillol. Monoboc-protected ethylendiamine will then be coupled to prepare intermediate-6. The product will be recovered and immediately be subjected to DCC/DMAP mediated coupling with PAzPC. The chemical identity of both analogues will be confirmed by NMR and mass-spectrometric anayses.

US11872313B2. Prodrug compositions, prodrug nanoparticles, and methods of use thereof (2024-01-16). Washington University [US]. Inventors: Gregory M. Lanza [US], Dipanjan Pan [US].
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Patent 2024
4-nitrophenyl Anabolism Atmosphere Camptothecin Carbonates Chromatography ethylenediamine Fatty Acids fumagillol Hexanes Lipids Mass Spectrometry Prodrugs
2
Genotoxic Stress Response in Yeast
Single colony derived yeast cells were incubated overnight at the appropriate temperature in liquid medium. Cells were diluted to 0.5 OD600 and spotted in ten-fold serial dilutions onto YPD plates, SC plates, or plates containing the indicated amount of genotoxic drugs, i.e., methylmethanesulfonate (MMS), camptothecin (CPT) or hydroxyurea (HU) (all drugs: Sigma-Aldrich). The agar plates were incubated at the indicated temperatures and time and imaged using the ChemiDoc™ Touch Imaging System (Bio-Rad).
Standard YPD agar has pH 5.5. To make YPD agar plates with alkaline pH, we titrated melted YPD agar with 10 N NaOH until pH 8.0 was reached. The wsc1::KAN knockout strain was used as a positive control for the alkaline agar plates28 (link).
Schindler N., Tonn M., Kellner V., Fung J.J., Lockhart A., Vydzhak O., Juretschke T., Möckel S., Beli P., Khmelinskii A, & Luke B. (2023). Genetic requirements for repair of lesions caused by single genomic ribonucleotides in S phase. Nature Communications, 14, 1227.
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Publication 2023
Agar Camptothecin Cells Hydroxyurea Methyl Methanesulfonate Pharmaceutical Preparations Strains Technique, Dilution Touch Yeasts
3
Antibody Characterization and DNA Damage Assays
Mouse antibody to MASTL (clone 4F9, Millipore MABT372) was described previously (Wang et al., 2011 (link)). Phospho-specific E6AP Ser-218 antibody was generated using a synthesize peptide (SSRIGDS phospho-S QGDNNLQ). Other antibodies include α-tubulin (Santa Cruz Biotechnology, #sc-5286), E6AP (Bethyl Laboratories, A300-351), HA (Cell signaling technology #3724), γ-H2AX Ser-139 (Cell signaling technology #9718S), phospho-ATM/ATR substrate motif (Cell signaling technology, #6966S), phospho-SMC1 Ser-957 (Cell signaling technology, #58052), phospho-CHK1 Ser-345 (Cell signaling technology, #2348), phospho-CHK2 Thr-68 (Cell signaling technology, #2197), Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (Cell signaling technology, #2914), Aurora A (Cell signaling technology, #14475), Aurora B (Cell Signaling technology, #3094), CDK1 (Cell signaling technology, #9112), Cyclin B (Cell signaling technology, #4138), phosphor-CDK substrates (Cell signaling technology, #2325), RPA32 (Thermo Fisher Scientific, # PA5-22256), S5a (Boston Biochem, #SP-400), ubiquitin (Cell Signaling Technology, #3936), and GFP (Cell Signaling Technology, #2555).
The following chemicals were used: Hydroxyurea (HU, MP Biomedicals, #102023), doxorubicin (DOX, MilliporeSigma, #25316-40-9), caffeine (Sigma-Aldrich, #C0750), ATM inhibitor (KU55933, Selleckchem, #S1092), ATR inhibitor (VE-821, Selleckchem, #S8007), cycloheximide (CHX, Fluka analytical, #01810), etoposide (Sigma-Aldrich, #E1383), camptothecin (Sigma-Aldrich, #C9911), G418 sulfate (Thermo Fisher Scientific, #10131035), MG132 (Calbiochem, #133407-82-6), cisplatin (R&D systems, #15663-27-1), propidium iodide (PI, Thermo Fisher Scientific, #P1304MP), and isopropyl-beta-D-thiogalactopyranoside (IPTG, RPI research products international, # 367-93-1).
Li Y., Wang F., Li X., Wang L., Yang Z., You Z, & Peng A. (2023). The ATM-E6AP-MASTL axis mediates DNA damage checkpoint recovery. bioRxiv.
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Publication Preprint 2023
alpha-Tubulin antibiotic G 418 Antibodies AURKA protein, human AURKB protein, human Caffeine Camptothecin CDK1 protein, human Cisplatin Clone Cells Cyclin B Cycloheximide Etoposide Hydroxyurea Immunoglobulins Isopropyl Thiogalactoside KU 55933 MG 132 Mus Peptides Phosphorus Propidium Iodide Sulfates, Inorganic Ubiquitin VE 821
4
Compound Screening for Chagas Disease
Compounds were prepared in 100% DMSO, at the following concentrations: benznidazole (supplied by EpiChem Pty Ltd., Perth, Australia): 34.7 mM, nifurtimox (Sigma Aldrich, St. Louis, MO, USA): 34.7 mM, ciclopirox olamine: 40 mM, camptothecin 20 mM, clotrimazole 17.5 mM (all purchased from Sigma Aldrich, USA). Ethyl oxo{[2-(4-phenyl-1-piperazinyl)-2-(3-pyridinyl)ethyl]amino} acetate: 20 mM (E155-0206; Chemdiv, San Diego, CA, USA). MMV688958 and MMV688796 were sourced from Enamine (Kyiv, Ukraine) and prepared at 20 mM in 100% DMSO. Compounds were tested in assays in 14 log dilutions to determine IC50 values, ranging from 73 µM to 3.7 × 10−3 µM for camptothecin, E155-0206, MMV688958 and MMV688796; 64.1 µM to 2.6 µM for clotrimazole; 146 µM to 2.6 µM for ciclopirox olamine and 127 µM to 6.4 × 10−3 µM for nifurtimox and benznidazole, to determine IC50 values.
Sykes M.L., Kennedy E.K, & Avery V.M. (2023). Impact of Laboratory-Adapted Intracellular Trypanosoma cruzi Strains on the Activity Profiles of Compounds with Anti-T. cruzi Activity. Microorganisms, 11(2), 476.
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Publication 2023
benznidazole Biological Assay Camptothecin Ciclopirox Olamine Clotrimazole ethyl acetate Nifurtimox Sulfoxide, Dimethyl Technique, Dilution
5
Cytotoxicity Evaluation of Cell Lines
Human embryonic kidney (HEK293), breast cancer (MCF-7) and human lung cancer (A549) cells were donated by the Department of Biotechnology, Durban University of Technology. Cells were grown at 37 °C in a humidified incubator under 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% Foetal Bovine Serum (FBS) and antibiotics (Penicillin; 10,000 U mL−1 and Streptomycin sulphate; 10,000 U mL−1 [Penicillin/Streptomycin]). Antibiotics change the phenotype and morphology of cells; therefore, the use of the antibiotics should be in very low concentrations, thus for this study, 1% Penicillin/Streptomycin was used. The cells were grown until 80% confluence was reached with media replaced as necessary. After confluence was reached, cells were trypsinized and sub-cultured. The 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cytotoxicity of the extracts. The MTT assay was conducted according to Dwarka et al. [40 (link)] with minor modifications. Briefly, cells (50 μL of 1 × 102 cells mL−1), as well as 50 μL of DMEM, were seeded into a 96-well flat bottom plate and incubated (37 °C for 24 h) in a humidified incubator under 5% CO2. Cells were then treated with 50 μL of extracts at varying concentrations (7.8–1000 μg mL−1) prepared in 5% DMSO and incubated for 24 h. Camptothecin was used as a positive control. MTT reagent (20 μL, 5 mg mL−1) was added to the cells and incubated at 37 °C for 4 h. One hundred microliters of DMSO was then added to each well to solubilise the formazan salt formed, and absorbance was read at 570 nm on a microplate spectrophotometer (Multiscan Go, Thermo Scientific, Waltham, MA, USA) for both treated and untreated cells. The percentage viability was calculated using the following formula: Cell viability (%)=Absorbance of treated cellsAbsorbance of untreated cells × 100
Adu O.T., Naidoo Y., Lin J., Dwarka D., Mellem J., Murthy H.N, & Dewir Y.H. (2023). Cytotoxic Potential of Diospyros villosa Leaves and Stem Bark Extracts and Their Silver Nanoparticles. Plants, 12(4), 769.
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Publication 2023
A549 Cells Antibiotics Biological Assay Breast Carcinoma Bromides Camptothecin Cells Cell Survival Cytotoxin Eagle Embryo Fetal Bovine Serum Formazans Homo sapiens Kidney Lung Cancer Penicillins Phenotype Sodium Chloride Streptomycin Streptomycin Sulfate Sulfoxide, Dimethyl

Top products related to «Camptothecin»

1
Camptothecin by Merck Group
Sourced in United States, Germany, United Kingdom, Belgium, Sao Tome and Principe, France, Macao, Poland
Camptothecin is a naturally occurring alkaloid compound isolated from the Camptotheca acuminata tree. It is a biologically active compound with potential applications in research and development. Camptothecin exhibits inhibitory effects on the enzyme topoisomerase I, which is involved in DNA replication and transcription processes. This property makes Camptothecin a valuable tool for studying cellular mechanisms and biological processes.
2
Etoposide by Merck Group
Sourced in United States, Germany, United Kingdom, Japan, France, China, Italy, Canada, Switzerland, Belgium, Macao, Portugal, Poland
Etoposide is a chemotherapeutic agent used in the treatment of various types of cancer. It is a topoisomerase inhibitor that disrupts the process of DNA replication, leading to cell death. Etoposide is available as a solution for intravenous administration.
3
Camptothecin cpt by Merck Group
Sourced in United States
Camptothecin (CPT) is a chemical compound derived from the Camptotheca acuminata tree. It is a potent inhibitor of the enzyme topoisomerase I, which is involved in DNA replication and transcription. Camptothecin and its derivatives have been extensively studied for their potential use in cancer treatment and other medical applications.
4
Dimethyl sulfoxide (dmso) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
5
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
6
Cisplatin by Merck Group
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Cisplatin is a platinum-based medication used as a chemotherapeutic agent. It is a crystalline solid that can be dissolved in water or saline solution for administration. Cisplatin functions by interfering with DNA replication, leading to cell death in rapidly dividing cells.
7
Camptothecin by Selleck Chemicals
Sourced in United States, Germany
Camptothecin is a naturally occurring quinoline alkaloid compound. It functions as a topoisomerase I inhibitor, which plays a crucial role in cellular processes such as DNA replication and transcription.
8
Hydroxyurea by Merck Group
Sourced in United States, Germany, France, Italy, United Kingdom
Hydroxyurea is a chemical compound used in various laboratory applications. It functions as an inhibitor of ribonucleotide reductase, an enzyme involved in the synthesis of DNA.
9
Doxorubicin by Merck Group
Sourced in United States, Germany, United Kingdom, France, China, India, Italy, Poland, Macao, Japan, Sao Tome and Principe, Canada, Spain, Brazil, Australia, Belgium, Switzerland, Singapore, Israel
Doxorubicin is a cytotoxic medication that is commonly used in the treatment of various types of cancer. It functions as an anthracycline antibiotic, which works by interfering with the DNA replication process in cancer cells, leading to their destruction. Doxorubicin is widely used in the management of different malignancies, including leukemia, lymphoma, and solid tumors.
10
Cycloheximide (chx) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, Japan, Spain, Canada, Switzerland, India, Israel, Brazil, Poland, Portugal, Australia, Morocco, Sweden, Austria, Senegal, Belgium
Cycloheximide is a laboratory reagent commonly used as a protein synthesis inhibitor. It functions by blocking translational elongation in eukaryotic cells, thereby inhibiting the production of new proteins. This compound is often utilized in research applications to study cellular processes and mechanisms related to protein synthesis.

More about "Camptothecin"

Camptothecin, a plant-derived alkaloid, has shown remarkable antitumor properties.
This potent compound inhibits the essential enzyme topoisomerase I, leading to the accumulation of DNA single-strand breaks and ultimately, cell death.
Camptothecin and its derivatives have demonstrated promising results in the treatment of various cancers, such as lung, ovarian, and colorectal cancers.
Researchers can leverage the PubCompare.ai platform to efficiently locate the best protocols from literature, preprints, and patents.
This AI-driven solution allows for seamless comparisons, helping identify the optimal methods and products for their Camptothecin research needs.
By utilizing this innovative platform, researchers can streamline their Camptothecin studies, enhancing the reproducibility and accuracy of their findings.
Camptothecin's mechanism of action involves inhibiting the topoisomerase I enzyme, which plays a crucial role in DNA replication and transcription.
This inhibition leads to the accumulation of DNA single-strand breaks, ultimately triggering cell death.
Etoposide, another anticancer agent, also targets topoisomerase II, a related enzyme, while Cisplatin and Hydroxyurea employ different mechanisms to induce cell death.
The use of DMSO (Dimethyl Sulfoxide) and FBS (Fetal Bovine Serum) are common in Camptothecin research, as they facilitate the solubility and cellular uptake of the compound.
Doxorubicin and Cycloheximide, on the other hand, are commonly used as positive controls in Camptothecin studies, as they possess their own potent anticancer properties.
By incorporating the insights gained from the MeSH term description and the Metadescription, researchers can optimize their Camptothecin studies, leveraging the power of the PubCompare.ai platform to streamline their research processes and enhance the reproducibility and accuracy of their findings.