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Capsaicin

Capsaicin, the pungent compound found in chili peppers, is a widely studied bioactive molecule with diverse therapeutic applications.
This AI-driven tool, PubCompare.ai, optimizes Capsaicin research by enhancing reproducibility and accuracy.
It helps researchers locate the best protocols from literature, pre-prints, and patents, using intelligent comparisons to identify the most effective methods and products.
Streamlining Capsaicin research, this powerful tool enables informed decisions and acheives better results, accelerating scientific progress in this important field.

Most cited protocols related to «Capsaicin»

1
Comparative Assessment of Mechanosensory Thresholds
Cited 111 times
All prospective mechanosensitivity experiments with animals were conducted in accordance with the guidelines from the Canadian Council on Animal Care and approval of the Laval University Animal Care Committee. PWT was measured in adult male C57BL/6 mice using von Frey filaments 2 through 9. Mice were placed in acrylic chambers (5.5 × 10 cm) suspended above a wire mesh grid and allowed to acclimatize to the testing apparatus for 1 hour prior to experiments. When the mouse was not moving the von Frey filaments were pressed against the plantar surface of the paw until the filament buckled and held for a maximum of 3 seconds. A positive response was noted if the paw was sharply withdrawn on application of the filament. Flinching immediately upon removal of the filament was also considered a positive response as previously described [3 (link)]. Testing began with filament number 5 and progressed according to an up-down method. Mice were randomly assigned to be tested either with the method of Chaplan et al. [3 (link)] or SUDO. After the first measurement of PWT, a second measurement of PWT was conducted using the alternate test. In some prospective experiments, hyperalgesia was induced by intraplantar injection of capsaicin (0.5% w/v, 5 μl) or complete Freund’s adjuvant (CFA; 10 μl) in one hind paw under brief anesthesia with isoflurane (<3 minutes, 4% isoflurane). PWT was measured 3 hours after intraplantar injection of either compound and again 3 days after injection of CFA. The PWT estimates derived using SUDO or the method of Chaplan et al. [3 (link)] for each condition were compared using a paired t-test in Graphpad Prism 5.
Bonin R.P., Bories C, & De Koninck Y. (2014). A simplified up-down method (SUDO) for measuring mechanical nociception in rodents using von Frey filaments. Molecular Pain, 10, 26.
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Publication 2014
Adult Anesthesia Animal Care Committees Animals ARID1A protein, human Capsaicin Cytoskeletal Filaments Freund's Adjuvant Hyperalgesia Isoflurane Males Mice, House Mice, Inbred C57BL prisma
2
Intensity Ratings of Taste and Chemesthetic Stimuli
Cited 32 times

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Publication 2012
Auditory Perception Capsaicin Osmosis Potassium Chloride Quinine Hydrochloride Savory Sodium Chloride Sucrose Taste Touch
3
TRPV1 Structural Rearrangements Revealed
Cited 15 times
The apo-structure of TRPV1 was docked into the 3D density maps of capsaicin- and RTX/DkTx-bound structures as a rigid body. The S1-S4 domain fits nicely into both maps, therefore only slight adjustments were performed at this part of the model. On the other hand, the S4-S5 linker, pore module (S5-P-S6) and TRP domain of the TRPV1 apo-structure exhibit substantial deviation from both maps, indicating structural rearrangements during ligand binding. This part of the model was rebuilt in Coot 53 (link),54 (link) using the apo-structure as a reference. The density map for the pore module of capsaicin bound structure is of sufficient quality to assign most side chains in this region, allowing us to obtain an accurate pore profile by calculating pore radii using HOLE program55 (link). However, density for the TRP domain, especially the part that immediately follows S6, does not allow for side chain resolution, likely reflecting the dynamic nature of this region during gating. Nonetheless, the invariant W697 in helical TRP domain shows excellent density and serves as a landmark to orient the entire helix. The map of RTX/DkTx bound structure is of sufficient quality to build the model without ambiguity. A polyalanine model (a total of 31 aa) based on the NMR structure of hanatoxin (pdb code: 1D1H) 56 (link) was docked onto the DkTx density with slight adjustment.
Cao E., Liao M., Cheng Y, & Julius D. (2013). TRPV1 structures in distinct conformations reveal mechanisms of activation. Nature, 504(7478), 113-118.
Publication 2013
AA 31 Apolipoproteins A Capsaicin Gene Rearrangement hanatoxin Helix (Snails) Human Body Ligands Microtubule-Associated Proteins Muscle Rigidity polyalanine Radius Seizures
4
Dissecting Substance P-Mediated Signaling
Cited 11 times
The study was designed to examine the contribution of SP-induced endocytosis of the NK1R to signal transduction in subcellular compartments, excitation of spinal neurons, and nociception. Endocytosis of the NK1R was examined in HEK293 cells by using BRET to assess the proximity between the NK1R and proteins resident in the plasma membrane and early endosomes and by localizing fluorescent SP by confocal microscopy. BRET was also used to examine the assembly of signaling complexes, which were localized in endosomes by immunofluorescence and super-resolution microscopy. Signaling in subcellular compartments of HEK293 cells was studied by expressing genetically encoded FRET biosensors, which allowed analysis of signaling with high spatial and temporal fidelity. NK1R endocytosis was studied in spinal neurons in slice preparations and in vivo by immunofluorescence and confocal microscopy. To examine the excitation of pain-transmitting neurons, cell-attached patch clamp recordings were made from second-order neurons in slices of rat spinal cord. Nociceptive behavior was evaluated in conscious mice after intraplantar administration of capsaicin, formalin, or CFA. To examine the contribution of NK1R endocytosis to signaling, neuronal excitation, and nociception, HEK293 cells, rat spinal cord slices, or mice were treated with pharmacological or genetic inhibitors of clathrin, dynamin, or βARRs, or with peptide inhibitors of NK1R/βARR interactions. Peptidic and small-molecule antagonists of the NK1R were conjugated to the lipid cholestanol, which facilitated endosomal targeting and retention of antagonists. Cholestanol-conjugated antagonists were used to directly evaluate the contribution of NK1R signaling in endosomes to SP-induced compartmentalized signaling in HEK293 cells, excitation of spinal neurons, and nociception. Institutional Animal Care and Use Committees approved all studies.
Jensen D.D., Lieu T., Halls M.L., Veldhuis N.A., Imlach W.L., Mai Q.N., Poole D.P., Quach T., Aurelio L., Conner J., Herenbrink C.K., Barlow N., Simpson J.S., Scanlon M.J., Graham B., McCluskey A., Robinson P.J., Escriou V., Nassini R., Materazzi S., Geppetti P., Hicks G.A., Christie M.J., Porter C.J., Canals M, & Bunnett N.W. (2017). Neurokinin 1 receptor signaling in endosomes mediates sustained nociception and is a viable therapeutic target for prolonged pain relief. Science translational medicine, 9(392), eaal3447.
Publication 2017
Aftercare antagonists Biosensors Capsaicin Cell Membrane Proteins Cells Cholestanol Clathrin Consciousness Dynamins Endocytic Vesicles Endocytosis Endosomes Fluorescence Resonance Energy Transfer Formalin HEK293 Cells Immunofluorescence inhibitors Institutional Animal Care and Use Committees Lipids Microscopy Microscopy, Confocal Mus Neurons Nociception Pain Peptides Reproduction Retention (Psychology) Spinal Cord
5
Respiratory Muscle Activity in Anesthetized Rats
Cited 11 times

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Publication 2010
Airway Obstruction Animals Capsaicin Dental Occlusion Hypoxia Neck Nerve Block Operative Surgical Procedures Oximetry, Pulse Phrenic Nerve Pulse Rate Rattus Respiratory Rate Silver

Most recents protocols related to «Capsaicin»

1
Chemosensitivity Assessment of 3D Cells
The chemosensitivity of the cells to a variety of conventional chemotherapeutic drugs, small molecule inhibitors, and natural products was determined using an MTT assay. The choice of chemotherapeutic agents was motivated by the desire to compare cell response between two models, 3D cells in the present study and semi-solid Matrigel-embedded cells in our previous study (22 (link)). Standard chemotherapeutic agents that target proliferating cell mechanisms, as well as drugs with multi-targeted actions that do not rely on the proliferative status of the cells, were used. Doxorubicin, etoposide, vinblastine, paclitaxel, 2-deoxyglucose (2-DG), emodin, apigenin, resveratrol, caffeic acid phenethyl ester (CAPE), curcumin, capsaicin, shikonin, and dihydroxybenzaldehyde (DHBZ) were purchased from MilliporeSigma. Cucurbitacin I (CBC-I), AG-490, and BAY 11-7085 were purchased from Calbiochem. Chrysin was kindly provided by Dr Sirivan Athikomkulchai (Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok, Thailand). Briefly, 100 µl of the cell suspension was seeded into each well of a 96-well plate (1×104 cells), then 100 µl of cytotoxic agents in a range of concentrations or a vehicle (cell culture media) were added. After 48 h of incubation, each well was replaced with 100 µl of 0.5 mg/ml MTT solution (MilliporeSigma) and incubated for another 2 h at 37°C. Absorbance was measured at 550 nm (650 nm was subtracted as the reference wavelength) using a microplate reader. The IC50 value for each cytotoxic drug (the drug concentration exhibiting 50% cell viability) was calculated.
Keeratichamroen S., Sornprachum T., Ngiwsara L., Ornnork N, & Svasti J. (2023). p‑STAT3 influences doxorubicin and etoposide resistance of A549 cells grown in an in vitro 3D culture model. Oncology Reports, 49(4), 71.
Publication 2023
AG-490 Antineoplastic Agents Apigenin BAY 11-7085 Biological Assay caffeic acid phenethyl ester Capsaicin Cell Culture Techniques Cells Cell Survival chrysin cucurbitacin I Culture Media Curcumin Cytotoxin Doxorubicin Drug Delivery Systems Emodin Etoposide Faculty, Pharmacy inhibitors matrigel Natural Products Paclitaxel Pharmaceutical Preparations Pharmacotherapy Resveratrol shikonin Vinblastine
2
Quantifying Capsaicinoids in Digested Emulsion Gels

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Publication 2023
acetonitrile Capsaicin Chromatography Digestion dihydrocapsaicin Emulsions Ethanol Gels High-Performance Liquid Chromatographies Intestines Liquid Chromatography Methanol Micelles
3
Simulated Digestion of Capsaicinoids

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Publication 2023
Aspergillus niger Bath Bile Bos taurus Capsaicin Digestion dihydrocapsaicin Food Intestines Lipase Mucosa, Gastric Pancreas Pancreatin Pefabloc Pepsin A Pigs Polysorbate 80 Proteins Soybean oil Stomach Tween 80 Whey Proteins
4
Thermal Stimulation and Capsaicin Sensitization
All heat stimuli were applied using a 25x50 mm contact thermode (SENSELab—MSA Thermotest, SOMEDIC Sales AB, Sweden). The baseline temperature was set to 30 °C. Rise and fall rates of the temperature were set to 5 °C/s. All thermal stimuli were applied to the inner forearm of participants’ non-dominant hand after sensitization of the skin using 0.075% topical capsaicin cream to allow for potent pain relief as reward and pain increase as punishment without the risk of skin damage (Becker et al., 2015 (link); Gandhi et al., 2013 (link)). By activating temperature-dependent TRPV1 (vanilloid transient receptor potential 1) ion channels capsaicin as the active ingredient of chili pepper induces heat sensitization (Holzer, 1991 (link)). To ensure that the entire area of thermal stimulation during the wheel of fortune game was sensitized the cream was applied to an area on the forearm exceeding the area of stimulation by about 1 cm on each side. After 20 min, the capsaicin cream was removed (Dirks et al., 2003 (link); Gandhi et al., 2013 (link)) and the thermode was applied. If participants reported the baseline temperature of the thermode (30 °C) as painful because of the preceding sensitization this temperature was lowered until it was perceived as non-painful, which was needed in 8 out of 83 sessions (3 placebo sessions, 1 levodopa session, and 4 naltrexone sessions) that were finally entered into the analysis (see below). The temperature was decreased to 28 °C (1 placebo session, 4 naltrexone sessions) or 26 °C (1 placebo session, 1 levodopa session). The need to lower the baseline temperature was not significantly different between drug conditions (Fisher’s exact test, P=0.52).
Desch S., Schweinhardt P., Seymour B., Flor H, & Becker S. (2023). Evidence for dopaminergic involvement in endogenous modulation of pain relief. eLife, 12, e81436.
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Publication 2023
Capsaicin Forearm Levodopa Naltrexone Pain Peppers, Chile Pharmaceutical Preparations Placebos Skin Transients TRPV Cation Channels
5
Neuronal response to ApoE isoforms
Cerebral cortices were harvested from newborn (P0–P2) C57BL/6 mice as previously described15 (link). Neurons were cultured with 150 nM human recombinant ApoE3 (PeproTech 350-02, NJ, USA) or ApoE4 (PeproTech 350-04) combined with 100 μM PA for 24 h. In some experiments, neurons were preincubated with 10 μM capsaicin for 30 min followed by the addition of ApoE3 or ApoE4 and PA. Human neuroblastoma-derived SH-sy5y cells were exposed to 150 nM human recombinant ApoE3 or ApoE4 combined with 100 μM PA for 24 h. Microglia-conditioned medium (MCM) was collected from the culture medium of human recombinant ApoE3- and ApoE4-treated BV2 cells. BV2 cells were preincubated with 10 μM capsaicin for 30 min, and then ApoE3 or ApoE4 was added. DIV8 neurons were incubated with 95% neuronal medium and 5% MCM at 37 °C with 5% CO2 for 24 h.
Wang C., Lu J., Sha X., Qiu Y., Chen H, & Yu Z. (2023). TRPV1 regulates ApoE4-disrupted intracellular lipid homeostasis and decreases synaptic phagocytosis by microglia. Experimental & Molecular Medicine, 55(2), 347-363.
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Publication 2023
Apolipoprotein E3 Apolipoprotein E4 Capsaicin Cells Cortex, Cerebral Culture Media, Conditioned Homo sapiens Infant, Newborn Mice, Inbred C57BL Microglia Neuroblastoma Neurons

Top products related to «Capsaicin»

1
Capsaicin by Merck Group
Sourced in United States, Germany, United Kingdom, Sao Tome and Principe, Japan, Italy, France, Canada, Brazil, Australia, Macao, Belgium, Ireland, Mexico
Capsaicin is a chemical compound found in various chili peppers. It is used as a laboratory reagent and is often employed in the study of pain perception and the somatosensory system. Capsaicin acts as an agonist for the TRPV1 receptor, which is involved in the detection of heat, pain, and certain pungent chemicals.
2
Capsazepine by Merck Group
Sourced in United States, Germany, France, Sao Tome and Principe
Capsazepine is a synthetic compound primarily used as a laboratory reagent. It functions as a competitive antagonist of the capsaicin receptor, also known as the transient receptor potential cation channel subfamily V member 1 (TRPV1). Capsazepine is utilized in research settings to study the role of TRPV1 in various physiological and pathological processes.
3
Capsaicin by Bio-Techne
Sourced in United Kingdom, United States
Capsaicin is a laboratory reagent used for various research applications. It is the primary active compound found in chili peppers and is known for its pungent properties. As a laboratory product, Capsaicin is utilized for its specific chemical and biological characteristics, without any interpretations or extrapolations on its intended use.
4
Dimethyl sulfoxide (dmso) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
5
Menthol by Merck Group
Sourced in United States, Germany, Spain, United Kingdom, Brazil, France, Belgium, China
Menthol is a crystalline organic compound derived from peppermint oil or other mint oils. It has a characteristic minty aroma and cooling sensation. Menthol is commonly used as a flavoring agent and has various industrial applications.
6
Fura 2 am by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Italy, France, Spain, Australia, Japan, China, Canada, Belgium, Austria, Hungary, Macao
Fura-2 AM is a fluorescent calcium indicator used for measuring intracellular calcium levels. It is a cell-permeable derivative of the parent compound Fura-2. Fura-2 AM can be loaded into cells, where intracellular esterases cleave off the acetoxymethyl (AM) ester group, trapping the Fura-2 indicator inside the cell.
7
Dihydrocapsaicin by Merck Group
Sourced in United States, Germany
Dihydrocapsaicin is a laboratory compound used in research applications. It is a naturally occurring analog of capsaicin, the primary pungent compound found in chili peppers. Dihydrocapsaicin serves as a research tool for studying various biological processes and mechanisms.
8
Cinnamaldehyde by Merck Group
Sourced in United States, Germany, Brazil, United Kingdom, Spain, Portugal, Australia, Sao Tome and Principe, India
Cinnamaldehyde is a chemical compound used in various laboratory applications. It is a pale yellow liquid with a characteristic cinnamon-like aroma. Cinnamaldehyde serves as a key ingredient in many fragrance and flavoring formulations.
9
Capsaicin by Fujifilm
Sourced in Japan
Capsaicin is a chemical compound found in chili peppers. It is a white crystalline substance that has a pungent, burning sensation when in contact with the skin or mucous membranes. Capsaicin is commonly used in laboratory equipment for various research and analytical purposes.
10
Indomethacin by Merck Group
Sourced in United States, Germany, United Kingdom, Brazil, Italy, Sao Tome and Principe, China, India, Japan, Denmark, Australia, Macao, Spain, France, New Zealand, Poland, Singapore, Switzerland, Belgium, Canada, Argentina, Czechia, Hungary
Indomethacin is a laboratory reagent used in various research applications. It is a non-steroidal anti-inflammatory drug (NSAID) that inhibits the production of prostaglandins, which are involved in inflammation and pain. Indomethacin can be used to study the role of prostaglandins in biological processes.

More about "Capsaicin"

Capsaicin, a pungent compound found in chili peppers, is a widely studied bioactive molecule with diverse therapeutic applications.
This compound, also known as 8-methyl-N-vanillyl-6-nonenamide, has gained significant attention in the scientific community due to its potential benefits in various areas, such as pain management, inflammation reduction, and metabolic regulation.
The AI-driven tool, PubCompare.ai, has been developed to optimize Capsaicin research by enhancing reproducibility and accuracy.
This powerful platform helps researchers locate the best protocols from literature, pre-prints, and patents, using intelligent comparisons to identify the most effective methods and products.
By streamlining Capsaicin research, PubCompare.ai enables informed decisions and acheives better results, accelerating scientific progress in this important field.
Related compounds, such as Capsazepine (a Capsaicin antagonist), DMSO (a solvent used to deliver Capsaicin), Menthol (a cooling agent with similarities to Capsaicin), Fura-2 AM (a fluorescent indicator used to measure Capsaicin-induced calcium influx), Dihydrocapsaicin (a structural analog of Capsaicin), and Cinnamaldehyde (a compound with potential analgesic properties), have also been studied in the context of Capsaicin research.
Indomethacin, a non-steroidal anti-inflammatory drug, has been used in combination with Capsaicin to investigate its effects on pain and inflammation.
Through the use of PubCompare.ai, researchers can optimze their Capsaicin studies, leading to more reproducible and accurate results that accelerate scientific progress in this important field of study.