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Captisol

Captisol is a patented, chemically modified cyclodextrin that enhances the solubility, stability, and bioavailability of pharmaceutical compounds.
It is commonly used in drug formulations to improve the delivery of poorly soluble drugs.
Captisol has been shown to increase the aqueous solubility of many drug molecules, making it a valuable tool for researchers developing new therapies.
Its unique chemical structure allows it to form inclusion complexes with a wide range of drugs, improving their physical and chemical properties.
Captisol is widely used in the pharmaceutical industry and has been approved for use in numerous drug products.
Researchers can utilze PubCompare.ai's AI-driven protocol comparisons to optimzie their Captisol reserach and boost reproducibility and accuracy.

Most cited protocols related to «Captisol»

1
Xenograft Tumor Growth Inhibition
Cited 6 times
Three million non-stem cancer cells (monolayer) or MCS cells (spheroid) were suspended in 100 μl phosphate buffered saline containing 30% matrigel followed by subcutaneous injection into the two front flanks of NOD scid IL2 receptor gamma chain knockout mice (NSG) using a 26.5 gauge needle. Five mice were used per treatment group. Tumor growth was monitored by measuring tumor diameter and calculating tumor volume = 4/3π × (diameter/2)3. For small molecular inhibitor studies, 3 million cells were injected into each front flank, and after 8 wks, when tumors were first palpable, inhibitor treatment was initiated by IP injection at final concentrations of 0, 20 and 50 mg/kg three times per week (M/W/F). A 0.3 M NC9 stock was prepared in DMSO and diluted into captisol at 2 and 4 mg/ml and 0.2 ml was IP injected per treatment for a final treatment concentration of 20 and 50 mg/kg body weight. NC9 treatment was initiated at the time tumors were detected at eight weeks post-injection. These studies were approved by the institutional board and followed accepted international practices for the treatment and welfare of animals.
Adhikary G., Grun D., Alexander H.R., Friedberg J.S., Xu W., Keillor J.W., Kandasamy S, & Eckert R.L. (2018). Transglutaminase is a mesothelioma cancer stem cell survival protein that is required for tumor formation. Oncotarget, 9(77), 34495-34505.
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Publication 2018
Body Weight Captisol Cells Injections, Intraperitoneal Interleukin Receptor Common gamma Subunit Malignant Neoplasms matrigel Mice, Knockout Mus Needles Neoplasms Phosphates Saline Solution SCID Mice Stem Cells Subcutaneous Injections Sulfoxide, Dimethyl
2
Combinatorial Therapy for Xenograft Tumors
Cited 4 times
When xenografts reached 100 mm3, mice were randomized to 1 of 4 groups (n=12/group): (1) the vehicle group (10% DMSO/10% captisol/PBS daily for 3 days by intraperitoneal injection); (2) the olaparib group (olaparib 50 mg/kg daily for 3 days by intraperitoneal injection); (3) the irradiation group (tumor-localized irradiation at a single dose of 10 Gy [RS320 irradiation system; 1.82 Gy/min; Gulmay Medical Ltd) as previously described (23) (link); and (4) the combination group (olaparib 50 mg/kg daily for 3 days, and tumor irradiation [10 Gy] given 30 minutes after the first dose of olaparib).
Three animals from each group were sacrificed at 24 or 72 hours after the first treatment, respectively, and tumors were processed for immunohistochemistry (IHC). The remaining animals (n=6/group) were monitored for tumor growth until the sizes of tumors reached 500 mm3 or 42 days after initiation of treatment, whichever occurred first.
Jiang Y., Verbiest T., Devery A.M., Bokobza S.M., Weber A.M., Leszczynska K.B., Hammond E.M, & Ryan A.J. (2016). Hypoxia Potentiates the Radiation-Sensitizing Effect of Olaparib in Human Non-Small Cell Lung Cancer Xenografts by Contextual Synthetic Lethality. International Journal of Radiation Oncology, Biology, Physics, 95(2), 772-781.
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Publication 2016
Animals Captisol Heterografts Immunohistochemistry Injections, Intraperitoneal Mus Neoplasms olaparib Radiotherapy Sulfoxide, Dimethyl
3
Ovarian Cancer Xenograft Modeling
Cited 3 times

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Publication 2017
Captisol Cells Chloride, Ammonium Cyclodextrins Erythrocytes Gamma Rays Heterografts Hypromellose Institutional Animal Care and Use Committees matrigel Mice, Inbred NOD Mus Neoplasms olaparib Peritoneum SCID Mice Solvents Tumor Burden Woman
4
Assessing Intratumoral Immune Responses
Cited 3 times

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Publication 2017
Alkaline Phosphatase Anti-Antibodies Antibodies BLZ-945 Brain Captisol Cell Cycle Checkpoints Cells Clodronate Cloning Vectors Combined Modality Therapy Cytotoxic T-Lymphocyte Antigen 4 Formalin Hamsters Immunoglobulin Isotypes Immunohistochemistry Liposomes Macrophage Mus Neoplasms Paraffin Embedding Psychological Inhibition Rabbits Tube Feeding
5
Orthotopic Mouse Model for Glioblastoma
Cited 3 times
All animal experiments were performed after obtaining IACUC approval, and by using UTHSCSA institution’s protocol guidelines. Male mice (athymic nude mice, homozygous) of 8 weeks’ old were purchased from Charles River (Wilmington, MO, USA). GSCs were labeled with GFP-Luciferase reporter and CD133-positive GSC10 (1 × 104) and U251-GSCs (5 × 104) were injected orthotopically into the mouse right cerebrum. Once the tumor is established, mice were randomized into control or treatment groups based on tumor volume. Investigators were not blinded in the animal studies. A sample size of mice/treatment was derived using information from previous studies and with the assumption of equal variance for the two groups and two-sided significance level 0.05. The control group received vehicle (30% Captisol) and the treatment group received NCL-1 (10 mg/kg body weight/day) or NCD-38 (5 mg/kg body weight/day) orally in 30% Captisol. Tumor growth was measured weekly using the Xenogen IVIS system. After treatment, the mice were euthanized, and brains were collected and processed for histological studies. For KDM1A knockdown experiments, GFP-Luciferase labeled U251-GSCs were infected with control shRNA or KDM1A-shRNA lentivirus and selected with puromycin (1 μg/ml) before injection. Mouse survival was determined using Kaplan–Meier survival curves and log-rank test using GraphPad Prism 6 software (GraphPad Software, San Diego, CA, USA).
Sareddy G., Viswanadhapalli S., Surapaneni P., Suzuki T., Brenner A, & Vadlamudi R. (2016). Novel KDM1A inhibitors induce differentiation and apoptosis of glioma stem cells via unfolded protein response pathway. Oncogene, 36(17), 2423-2434.
Publication 2016
Animals Body Weight Brain Captisol Cerebral Hemisphere, Right Homozygote Institutional Animal Care and Use Committees KDM1A protein, human Lentivirus Luciferases Males Mice, Nude Mus Neoplasms prisma Puromycin Rivers Short Hairpin RNA Specimen Handling

Most recents protocols related to «Captisol»

1
Genetic Modulation of Lung Tumor Growth
KrasLSL–G12D/+ transgenic mice were obtained from the Jackson Laboratory. The Setd2fl/fl mice were generated by Beijing Biocytogen Co. Ltd. KrasLSL–G12D/+ and Setd2fl/fl mice were bred to generate KrasLSL–G12D/+ Setd2fl/+ and KrasLSL–G12D/+ Setd2fl/fl mice. All animals were maintained on a mixed C57BL/6J × 129SvJ genetic background. Intranasal instillation of 2.5 × 107 plaque-forming units (pfu) of adenovirus-expressing Cre (Viral Vector Core Facility, University of Iowa, Iowa City, Iowa, USA) was performed on mice at 6–10 weeks of age as previously described (49 (link)). Tumor growth was monitored by MRI scans. Sex-matched KrasLSL–G12D/+ Setd2fl/fl mice, 4 weeks after adeno-Cre infection, were randomized into vehicle control and Dinaciclib or CBL0137 treatment groups. CBL0137 (Selleck Chemicals) was formulated in 50 mg/mL Captisol and administered i.v. twice weekly at 60 mg/kg for 4 weeks. Dinaciclib (Selleck Chemicals) was formulated in 20% hydroxypropyl β-cyclodextrin (MilliporeSigma) and administered i.p. 3 times weekly at 20 mg/kg. Lung tumor growth was assessed by MRI scans at 7 weeks after the first treatment. The body weights of the mice were monitored twice weekly.
Xie Y., Sahin M., Wakamatsu T., Inoue-Yamauchi A., Zhao W., Han S., Nargund A.M., Yang S., Lyu Y., Hsieh J.J., Leslie C.S, & Cheng E.H. (2023). SETD2 regulates chromatin accessibility and transcription to suppress lung tumorigenesis. JCI Insight, 8(4), e154120.
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Publication 2023
Adenovirus Vaccine Animals Body Weight Captisol CBL0137 Cloning Vectors Cyclodextrins dinaciclib Genetic Background Hypromellose Infection Lung Neoplasms Mice, Laboratory Mice, Transgenic MRI Scans Neoplasms Senile Plaques
2
Pharmacological Evaluations of Novel Compounds
The following drugs were used in our experiments: polyinosinic–polycytidylic potassium salt (Sigma, P9582-5MG, batch #0000080809), JNJ47965567 (Tocris Bioscience), ITH15004 (personal contribution from Instituto Teófilo Hernando), and ARL 67156 trisodium salt (Tocris Bioscience, 1283, batch #16A/253182). All drugs were dissolved in sterile saline, except for JNJ47965567 and ITH15004, which were dissolved in 30% Captisol solution (sulfobutyl ether-7-cyclodextrin) and 5% DMSO, respectively.
Mut-Arbona P., Huang L., Baranyi M., Tod P., Iring A., Calzaferri F., de los Ríos C, & Sperlágh B. (2023). Dual Role of the P2X7 Receptor in Dendritic Outgrowth during Physiological and Pathological Brain Development. The Journal of Neuroscience, 43(7), 1125-1142.
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Publication 2023
ARL-67156 Captisol Cyclodextrins Ethyl Ether ITH15004 Pharmaceutical Preparations Potassium Saline Solution Sodium Chloride Sterility, Reproductive Sulfoxide, Dimethyl
3
Rat Model of Middle Cerebral Artery Occlusion
The model has been reported in detail elsewhere [15 (link)]. In short, Wistar pup rats from each litter were randomly assigned to MCAO or control. All experimental groups were gender balanced. Each MCAO animal (MCAO, n = 133) was anesthetized by sevoflurane (5% induction, 1% maintenance). The left carotid artery was dissected up to the internal and external branches division to introduce a nylon filament 0.21 mm in diameter, which was inserted 8.5–9 mm through the internal carotid artery until left MCA occlusion was attained. All procedures lasted less than 30 min during which rectal temperature was kept at 38 ± 0.5 °C using a servo-controlled heat mattress. The occlusion was maintained for 3 h. Pups were anesthetized similarly to carefully remove the filament. After sealing carotid and skin wounds the pup then returned to the dam. Control group pups (SHAM, n = 112) were similarly managed but without MCAO.
Some MCAO rats received the DA reuptake inhibitor GBR-12909 dihydrochloride (MCAO + GBR, n = 22), the well-established effect of which is to increase DA concentration in the brain [24 (link)]. A stock solution of GBR-12909 2.5 mg/mL was obtained by dissolving 50 mg of GBR 12909 in 2 mL of dimethylsulfoxide (Sigma Aldrich, St. Louis, MO, USA), then adding 18 mL of 20% Captisol® (Abmole Bioscience, Dallas, TX, USA) and storing at −20 °C. An appropriate volume of the stock solution was further diluted in 0.9% saline to administer 5 mg/kg in 100 µL to each rat in a daily i.p. injection from P11 to P17.
The number of animals for each experiment is shown in Supplementary Materials (Table S1).
Villa M., Martínez-Vega M., del Pozo A., Muneta-Arrate I., Gómez-Soria A., Muguruza C., de Hoz-Rivera M., Romero A., Silva L., Callado L.F., Casarejos M.J, & Martínez-Orgado J. (2023). The Role of the Dopamine System in Post-Stroke Mood Disorders in Newborn Rats. International Journal of Molecular Sciences, 24(4), 3229.
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Publication 2023
Animals Brain Captisol Carotid Arteries Common Carotid Artery Cytoskeletal Filaments Dental Occlusion GBR 12909 Internal Carotid Arteries Normal Saline Nylons Rats, Wistar Rectum Sevoflurane Skin Sulfoxide, Dimethyl Wounds
4
Synthesis of OXA Analog CIDD-72229

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Publication 2023
Captisol High-Performance Liquid Chromatographies Pharmaceutical Preparations potassium phosphate Sodium Acetate Sodium Citrate
5
Modeling Kras/Trp53/Ezh2 Lung Cancer in Mice
All care and treatment of experimental animals were in accordance with Boston Children’s Hospital and University of Kentucky institutional animal care and use committee (IACUC) approved protocols. Cohorts of LSL:KrasG12D/+; Trp53flox/flox; Ezh2flox/flox (LSL: lox-stop-lox) genetically modified mouse models49 (link),50 (link) were maintained in virus-free conditions on a mixed C57BL/6x129SVj background. Mice were bred predominately as Ezh2fl/+ so that littermates and close relatives of the three Ezh2 genotypes could be analyzed for differential survival, metastasis, and tumor burden studies. For survival, mice that had healthy body characteristics at time of sacrifice were excluded from analysis. Both male and female mice were used for all experiments and no sex differences were noted. Adult mice received 2.5–2.9 × 107 PFU adeno-Cre by intranasal instillation. Mice were evaluated by Magnetic resonance imaging (MRI) 12 weeks after adeno-Cre infection for baseline scans. For Kras/Trp53/Ezh2 mouse models, JQ1 in aqueous suspension were made by diluting DMSO stock dropwise with 1:1 w/w Captisol in sterile saline while vortexing and was i.p. injected at 50 mg/kg q.d. for 2 weeks. For Nude mouse allograft experiment, Kras/Trp53/Ezh2 mouse tumor spheroids were dissociated into single cells, counted, and resuspended at 1 × 105 cells per 200 μL of 1:1 media/Matrigel (Corning, #356231). Female Athymic Nude Foxn1Nu/Nu mice (Envigo) were injected subcutaneously with 1 × 105 cells in 2–4 spots on flanks. Tumors were allowed to grow for 21 days to a mean size of 60 mm3. Mice were then randomized into groups that received GSK-J4, JQ1 or vehicle. 100 mg/kg GSK-J4 or 50 mg/kg JQ1 dissolved with 100% DMSO were administered by i.p. injection q.d. for 10 consecutive days. Tumors were measured by electronic caliper and tumor diameters were used to calculate tumor volumes (tumor volume = (length × width2)/2).
Chen F., Byrd A.L., Liu J., Flight R.M., DuCote T.J., Naughton K.J., Song X., Edgin A.R., Lukyanchuk A., Dixon D.T., Gosser C.M., Esoe D.P., Jayswal R.D., Orkin S.H., Moseley H.N., Wang C, & Brainson C.F. (2023). Polycomb deficiency drives a FOXP2-high aggressive state targetable by epigenetic inhibitors. Nature Communications, 14, 336.
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Publication 2023
Adult Allografts Animals Captisol CARE protocol Cells Exanthema EZH2 protein, human Genotype GSK-J4 Human Body Infection K-ras Genes Males matrigel Mus Neoplasm Metastasis Neoplasms Radionuclide Imaging Saline Solution Sterility, Reproductive Sulfoxide, Dimethyl Therapies, Investigational TP53 Gene Tumor Burden Virus Diseases Woman

Top products related to «Captisol»

1
Matrigel by BD
Sourced in United States, United Kingdom, Germany, China, Canada, Japan, Italy, France, Belgium, Australia, Uruguay, Switzerland, Israel, India, Spain, Denmark, Morocco, Austria, Brazil, Ireland, Netherlands, Montenegro, Poland
Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. It is widely used as a substrate for the in vitro cultivation of cells, particularly those that require a more physiologically relevant microenvironment for growth and differentiation.
2
Dimethyl sulfoxide (dmso) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
3
Mk 2206 by Selleck Chemicals
Sourced in United States, China, United Kingdom, Germany
MK-2206 is a selective allosteric Akt inhibitor that binds to the pleckstrin homology (PH) domain of Akt and inhibits its phosphorylation and activation. It has been used in research applications to study the role of Akt signaling in various cellular processes.
4
Captisol by Selleck Chemicals
Sourced in United States, Germany
Captisol is a chemically modified cyclodextrin used as a drug delivery excipient. It is designed to improve the solubility, stability, and bioavailability of pharmaceutical compounds.
5
Captisol by MedChemExpress
Captisol is a chemically modified cyclodextrin that is used to improve the solubility, stability, and bioavailability of various pharmaceutical compounds. It functions as a drug delivery excipient, enhancing the solubilization and formulation of poorly soluble drugs.
6
Matrigel by Corning
Sourced in United States, China, Germany, United Kingdom, Canada, Japan, France, Netherlands, Montenegro, Switzerland, Austria, Australia, Colombia, Spain, Morocco, India, Azerbaijan
Matrigel is a complex mixture of extracellular matrix proteins derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is widely used as a basement membrane matrix to support the growth, differentiation, and morphogenesis of various cell types in cell culture applications.
7
Tween 80 by Merck Group
Sourced in United States, Germany, United Kingdom, India, Italy, France, Sao Tome and Principe, Spain, Poland, China, Belgium, Brazil, Switzerland, Canada, Australia, Macao, Ireland, Chile, Pakistan, Japan, Denmark, Malaysia, Indonesia, Israel, Saudi Arabia, Thailand, Bangladesh, Croatia, Mexico, Portugal, Austria, Puerto Rico, Czechia
Tween 80 is a non-ionic surfactant and emulsifier. It is a viscous, yellow liquid that is commonly used in laboratory settings to solubilize and stabilize various compounds and formulations.
8
Captisol by Merck Group
Captisol is a patented, chemically modified cyclodextrin molecule developed by Cydex Pharmaceuticals, a subsidiary of Ligand Pharmaceuticals. It is designed to improve the solubility, stability, and/or bioavailability of drugs.
9
Erlotinib by Selleck Chemicals
Sourced in United States, China, Germany, Australia
Erlotinib is a laboratory reagent used in research applications. It is a tyrosine kinase inhibitor that targets the epidermal growth factor receptor (EGFR). Erlotinib is commonly used in cell-based assays and in vitro studies to investigate EGFR signaling pathways.
10
Mk2206 by MedChemExpress
Sourced in United States, China, Germany
MK2206 is a laboratory compound used for research purposes. It functions as an inhibitor of the AKT kinase, a key regulator of cellular processes such as metabolism, proliferation, and survival. The core function of MK2206 is to modulate AKT activity in experimental settings. No additional details or interpretations about the intended use of this product are provided.

More about "Captisol"

Captisol is a chemically modified cyclodextrin that enhances the solubility, stability, and bioavailability of pharmaceutical compounds.
It is commonly used in drug formulations to improve the delivery of poorly soluble drugs.
Captisol has been shown to increase the aqueous solubility of many drug molecules, making it a valuable tool for researchers developing new therapies.
Its unique chemical structure allows it to form inclusion complexes with a wide range of drugs, improving their physical and chemical properties.
Captisol is widely used in the pharmaceutical industry and has been approved for use in numerous drug products.
Researchers can utilize AI-driven protocol comparisons from PubCompare.ai to optimize their Captisol research and boost reproducibility and accuracy.
This powerful tool helps researchers locate the best protocols from literature, pre-prints, and patents, and provides intelligent analysis to enhance research outcomes.
Captisol is often used in conjunction with other excipients like Matrigel, DMSO, Tween 80, and MK-2206 to further improve drug delivery and formulation.
Researchers can leverage PubCompare.ai's advanced analytics to compare and optimize the use of these complementary compounds, leading to more effective and reproducible results.
PubCompare.ai's AI-driven protocol comparisons can also be applied to research involving Erlotinib and MK2206, two widely studied pharmaceutical compounds.
By systematically analyzing and comparing the best protocols from the literature, researchers can unlock new insights and streamline their drug development processes.
Overall, the utilization of Captisol and complementary excipients, combined with the power of PubCompare.ai's intelligent analysis, can significantly enhance the outcomes of pharmaceutical research and drug discovery efforts.
Experiance the benefits of this powerful tool and take your Captisol research to new heights.