Carmine red, which cannot be absorbed from the lumen of the gut, was used to study total GI transit time (Kimball et al., 2005 (link)). A solution of carmine red (300 µl; 6%; Sigma-Aldrich) suspended in 0.5% methylcellulose (Sigma-Aldrich) was administered by gavage through a 21 gauge round-tip feeding needle. The time at which gavage took place was recorded as T0. After gavage, fecal pellets were monitored at 10 min intervals for the presence of carmine red. Total GI transit time was considered as the interval between T0 and the time of first observance of carmine red in stool.
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Carmine
Carmine
Carmine is a natural red dye derived from the dried bodies of the female cochineal insect.
It is used in a variety of applications, including food coloring, cosmetics, and textiles.
PubCompare.ai's AI-driven research protocol comparison can help users enhance reproducibility and accuracy when working with Carmine by locating the best protocols from literature, pre-prints, and patents using intelligent comparison tools.
Optimize your Carmine research with PubCompare.ai's cutting-degge technology and unlock new insights.
It is used in a variety of applications, including food coloring, cosmetics, and textiles.
PubCompare.ai's AI-driven research protocol comparison can help users enhance reproducibility and accuracy when working with Carmine by locating the best protocols from literature, pre-prints, and patents using intelligent comparison tools.
Optimize your Carmine research with PubCompare.ai's cutting-degge technology and unlock new insights.
Most cited protocols related to «Carmine»
Carmine
Feces
Methylcellulose
Needles
Pellets, Drug
Transits, Gastrointestinal
Tube Feeding
Animals
Biological Assay
Carmine
Feces
Intestines
Light
Methylcellulose
Mice, House
Sterility, Reproductive
Tube Feeding
Protocol full text hidden due to copyright restrictions
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Carmine
Feces
Mice, House
Tube Feeding
Biological Assay
Carmine
Feces
Fenclonine
Methylcellulose
Mus
Sterility, Reproductive
Transits, Gastrointestinal
Tube Feeding
5-bromo-4-chloro-3-indolyl beta-galactoside
Carmine
Cells
E-600
Fluorescence
Grafts
Infection
Microscopy
Mus
Pad, Fat
Transplantation
Most recents protocols related to «Carmine»
The adult worms recovered after 37 days of mouse infection were fixed and stored in Alcohol-Formalin-Acetic Acid (AFA, 95% ethanol, 3% formaldehyde, and 2% glacial acetic acid), at room temperature, and stained with 2.5% chloride carmine, dehydrated in alcoholic series (70, 90%, and absolute), clarified in methyl salicylate with Canadian balsam (1:2), and prepared as whole-mounts (Neves et al., 1998 (link)). We analyzed at least six males and six females recovered from mice infected with schistosomula previously exposed to Smcarm1-, GFP-dsRNA, or untreated, from the three biological replicates.
Computer images (Image Pro Plus, Media Cybernetics), from male and female worms captured by a camera (640/480 pixels, RGB) coupled to a light microscope (BX50, Olympus), were used for morphometric analyses. We evaluated the number and area of testicular lobes, ovary area, presence of tubercles, presence of eggs and vitelline glands, and integrity of the tegument (Neves et al., 2004 (link)).
Whole mounts of male and female worms were also analyzed under confocal laser scanning microscopy (LSM-410, Zeiss) using a 543 nm laser and a BP560-615 IR filter, in reflected mode. We examined male (testicular lobes, seminal vesicle) and female (yolk glands, ovary, uterus, and ootype) reproductive systems, as well as the integrity of the tegument and the shape of the oral and ventral suckers.
Computer images (Image Pro Plus, Media Cybernetics), from male and female worms captured by a camera (640/480 pixels, RGB) coupled to a light microscope (BX50, Olympus), were used for morphometric analyses. We evaluated the number and area of testicular lobes, ovary area, presence of tubercles, presence of eggs and vitelline glands, and integrity of the tegument (Neves et al., 2004 (link)).
Whole mounts of male and female worms were also analyzed under confocal laser scanning microscopy (LSM-410, Zeiss) using a 543 nm laser and a BP560-615 IR filter, in reflected mode. We examined male (testicular lobes, seminal vesicle) and female (yolk glands, ovary, uterus, and ootype) reproductive systems, as well as the integrity of the tegument and the shape of the oral and ventral suckers.
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Acetic Acid
Adult
Alcoholics
Alcohols
Biopharmaceuticals
Carmine
Chlorides
Eggs
Ethanol
Females
Formaldehyde
Formalin
Genitalia
Helminths
Infection
Light Microscopy
Males
methyl salicylate
Mice, House
Microscopy, Confocal
Ovary
RNA, Double-Stranded
Seminal Vesicles
Testis
Uterus
Whole mammary glands were spread on a glass slide and fixed with Carnoy’s fixative (60% ethanol, 30% chloroform, 10% glacial acetic acid) overnight at RT. Fixed tissue was rehydrated by washing with decreasing ethanol concentrations (70%, 50%, 30%, 10%) 2 times each for 10 min. Rehydrated tissue was then stained with Carmine Alum (Stem Cell Technologies, 07,070) for 48–72 h. Mammary glands were then dehydrated using increasing ethanol concentrations (70%, 95%, 100%) 2 times each for 15 min, and cleared in xylene overnight. Cleared mammary glands were then mounted with Permount mounting medium (Fischer Chemical, SP15-100) and glass coverslips and allowed to dry overnight. Slides were imaged on the PerkinElmer Vectra3 slide scanner.
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Acetic Acid
alum, potassium
Carmine
Chloroform
Ethanol
Fixatives
Mammary Gland
Stem Cells
Tissues
Xylene
Three experimental batches of 50 capsules containing 20 mg of erythromycin were formulated with using a weight-based method. Traditionally, our capsules were compounded with a volume-based method, but recent findings have encouraged us to use a weight-based method for hospital preparations [23 (link)]. First, the tapped density of the erythromycin/cellulose mixture was found to be equal to 0.460 g.mL-1. One gram of pharmaceutical-grade erythromycin (Cooper® France) and 3.8 grams of microcrystalline cellulose (Cooper® France) were weighed on a qualified precision balance (Precisa XT220A, Precisa®). Approximatively 2 mg ± 0.4 mg (one spatula tip) of red carmine (Fagron® France) were added as a homogenization tracer. The mixture was transferred to a mortar for gentle mixing with a pestle. Fifty hard gelatin capsules (size 4, ivory) were placed on a manual capsule-filling machine (ProFiller 3700, LGA®). The caps were separated from the empty bodies and the entire mixture was inserted into the capsule bodies. The caps were then replaced, and the capsules were sealed. Routinely, these quantities were multiplied by 6 to obtain batches of 300 hard gelatin capsules.
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Capsule
Carmine
Cellulose
Erythromycin
Gelatins
Human Body
microcrystalline cellulose
Pharmaceutical Preparations
The fifth inguinal mammary gland was removed from the mouse, according to [25 (link)], and placed on a positively charged slide (StatLab, McKinney, TX, USA). It was subsequently fixed in Carnoy’s solution (Fisher Scientific, Pittsburgh, PA, USA) overnight and stained in a Carmine solution, prepared as previously described. Following staining, tissue was dehydrated with increasing ethanol, 70–100%, for 15 min each. To clear, tissue was placed in xylene for 12–72 h. Once the tissue became transparent, it was mounted using Permount Mounting Medium (Fisher Scientific, Pittsburgh, PA, USA). Images were captured using an Olympus VS120 Slide Scanner microscope (Olympus Lifescience, Center Valley, PA, USA) with 10×U Plan S Apo/0.75 NA objective. Quantification of mammary gland branching, and terminal end buds, was conducted, as previously described [25 (link)], using five mice per genotype and five randomly selected standard size fields of view per mouse.
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Carmine
Ethanol
Genotype
Groin
Mammary Gland
Mice, Laboratory
Microscopy
Tissues
Xylene
The behavioural phenomena of oxaliplatin-induced nausea and vomiting were evaluated via measurement of the pica response. Mice do not vomit but show an equivalent measurable behaviour called pica, defined as eating non-food items such as bedding material. Thus, pica is an indirect marker of emesis that was measured by the ingestion of kaolin clay (a non-nutritive substance). Mice ingest kaolin as an innate curative response for GI disturbances. Oxaliplatin-induced pica was quantified by measuring the consumption of kaolin pellets. Kaolin pellets were prepared as previously described [14 (link),15 (link)]. Briefly, kaolin (hydrated aluminum silicate; 98.5%) was mixed with 0.5% carmine and 1% gum Arabic to form a thick paste. The paste was then processed such that the shape and size of the kaolin pellets resembled that of the normal laboratory mouse food pellets.
After three days of acclimatization to the presence of kaolin (day 0), mice received i.p. injections either of oxaliplatin or saline, with kaolin and normal feed given immediately after i.p. injections. The amount of kaolin versus food intake was measured daily to observe the presence of abnormal eating patterns. Feces were collected at 0, 3, 7, and 14 days. After collection, fecal samples were counted and homogenised to determine the carmine concentration at absorbances of 550 and 700 nm using a spectrophotometer (PharmaSpec UV1700, Shimadzu, Japan).
After three days of acclimatization to the presence of kaolin (day 0), mice received i.p. injections either of oxaliplatin or saline, with kaolin and normal feed given immediately after i.p. injections. The amount of kaolin versus food intake was measured daily to observe the presence of abnormal eating patterns. Feces were collected at 0, 3, 7, and 14 days. After collection, fecal samples were counted and homogenised to determine the carmine concentration at absorbances of 550 and 700 nm using a spectrophotometer (PharmaSpec UV1700, Shimadzu, Japan).
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Acclimatization
Aluminum
Carmine
Clay
Feces
Food
Gum Arabic
Kaolin
Mice, Laboratory
Mus
Nausea
Oxaliplatin
Paste
Pellets, Drug
Pica
Saline Solution
Silicates
Vomiting
Top products related to «Carmine»
Sourced in United States, Germany, Italy
Carmine red is a natural dye derived from the dried bodies of the female cochineal insects. It is a vibrant red pigment commonly used in various laboratory applications as a coloring agent, stain, and indicator.
Sourced in United States, Germany, United Kingdom, Japan, Belgium, Switzerland, Italy, Australia, Canada, China, Sao Tome and Principe, Poland, Israel, France, Spain, Austria
Methylcellulose is a water-soluble, synthetic polymer derived from cellulose. It is a white, odorless, and tasteless powder that is commonly used as a thickening, stabilizing, and emulsifying agent in various industries, including pharmaceutical, food, and personal care products.
Sourced in United States
Carmine is a natural red colorant derived from the cochineal insect. It is used as a coloring agent in various laboratory applications, including staining and visualization techniques.
Sourced in United States, Canada, Japan, Germany, United Kingdom, Gabon, China
Permount is a mounting medium used in microscopy to permanently mount specimens on glass slides. It is a solvent-based, xylene-containing solution that dries to form a clear, resinous film, securing the specimen in place and providing optical clarity for microscopic examination.
Sourced in United States
Carmine alum is a laboratory chemical compound that serves as a mordant, which is a substance used to set dyes in various applications. It is a reddish-purple crystalline solid with a chemical formula of C₁₄H₈Al₃(OH)₂₄·nH₂O. Carmine alum is commonly used in histology, microscopy, and other scientific applications that require a stable and reliable coloring agent.
Sourced in Canada, Italy
Carmine Alum is a staining reagent used in histology and microscopy applications. It is a complex formed between carmine, a red pigment derived from the cochineal insect, and aluminum salts. Carmine Alum is used to stain nuclei and cytoplasm in tissue sections and cell preparations.
Sourced in United States
Carmine red dye is a natural pigment extracted from the dried bodies of the female cochineal insect. It is a vibrant, red-colored dye commonly used in the food, cosmetic, and textile industries. The dye's core function is to provide a stable, long-lasting red coloration to various products.
The C1022 is a piece of laboratory equipment manufactured by Merck Group. It is designed to perform a core function within the laboratory setting. The detailed specifications and intended use of this product are not available in this factual and unbiased description.
Sourced in Japan, United Kingdom, Germany
The GIF-Q260J is a laboratory equipment product from Olympus. It is designed to perform a core function, but the specific details of its intended use are not available in this concise, unbiased, and factual description.
Sourced in United States, Canada, Germany, Poland, Japan
Canada balsam is a naturally occurring resin extracted from the bark of the balsam fir tree. It is a clear, viscous liquid that has been used as a mounting medium and adhesive in various laboratory applications, such as slide preparation for microscopy. Canada balsam exhibits optical properties that make it suitable for use in certain scientific and technical applications.
More about "Carmine"
Carmine, a natural red dye derived from the dried bodies of the female cochineal insect, is widely used in a variety of applications, including food coloring, cosmetics, and textiles.
This vibrant pigment has a rich history and diverse applications.
Beyond its use as a colorant, Carmine also has medicinal properties and is sometimes used in pharmaceutical formulations.
Closely related terms include Carmine red, Carmine alum, and Carmine Alum, all of which refer to different forms or derivatives of this versatile dye.
Researchers working with Carmine can enhance the reproducibility and accuracy of their studies by utilizing AI-driven research protocol comparison tools like those offered by PubCompare.ai.
These intelligent comparison tools help users locate the best protocols from literature, pre-prints, and patents, unlocking new insights and optimizing Carmine research.
Additonally, Carmine is sometimes used in conjunction with other compounds, such as Methylcellulose and Permount, to create specialized formulations for various applications.
The unique properties of Carmine, combined with its wide-ranging uses, make it a fascinatiing subject of study.
Whether you're working with Carmine red dye, the C1022 or GIF-Q260J variants, or exploring the historical and cultural significance of this natural pigment, PubCompare.ai's cutting-edfe technology can help you navigate the landscape of Carmine research and unlock new discoveries.
This vibrant pigment has a rich history and diverse applications.
Beyond its use as a colorant, Carmine also has medicinal properties and is sometimes used in pharmaceutical formulations.
Closely related terms include Carmine red, Carmine alum, and Carmine Alum, all of which refer to different forms or derivatives of this versatile dye.
Researchers working with Carmine can enhance the reproducibility and accuracy of their studies by utilizing AI-driven research protocol comparison tools like those offered by PubCompare.ai.
These intelligent comparison tools help users locate the best protocols from literature, pre-prints, and patents, unlocking new insights and optimizing Carmine research.
Additonally, Carmine is sometimes used in conjunction with other compounds, such as Methylcellulose and Permount, to create specialized formulations for various applications.
The unique properties of Carmine, combined with its wide-ranging uses, make it a fascinatiing subject of study.
Whether you're working with Carmine red dye, the C1022 or GIF-Q260J variants, or exploring the historical and cultural significance of this natural pigment, PubCompare.ai's cutting-edfe technology can help you navigate the landscape of Carmine research and unlock new discoveries.