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Cetrimide

Cetrimide is a quaternary ammonium compound commonly used as an antimicrobial agent and surfactant.
It has broad-spectrum activity against bacteria, fungi, and viruses, making it a versatile compound in various applications such as disinfection, personal care products, and pharmaceutical formulations.
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This tool harnesses the power of AI-driven comparisons to enhance reproducibility and accuracy in Cetrimide studies, enabling researchers to discover the optimal Cetrimide protocols and maximize their research impact.

Most cited protocols related to «Cetrimide»

Anonymized clinical strains S. aureus PECHA 10 and P. aeruginosa PECHA 4, derived from patients with chronic wounds12 (link), were used in this study. These bacteria, coming from the private collection of the Bacteriological Laboratory of the Pharmacy Department, University “G. d’Annunzio” Chieti-Pescara, were cultured on Mannitol Salt Agar (MSA, Oxoid, Milan, Italy) and Cetrimide Agar (CET, Oxoid, Milan, Italy), respectively.
For the experiments, bacteria were cultured in Trypticase Soy Broth (TSB, Oxoid, Milan, Italy) and incubated at 37 °C overnight in aerobic condition and then refreshed for 2 h at 37 °C in an orbital shaker in aerobic condition. The cultures were standardized to on Optical Density at 600 nm (OD600) = 0.125 and diluted 1:10 for S. aureus PECHA 10 and 1:100 for P. aeruginosa PECHA 4, to obtain 106 CFU/ml and 105 CFU/ml, respectively.
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Publication 2020
Agar Bacteria Bacteria, Aerobic Cetrimide Mannitol Orbital Diseases Patients Pseudomonas aeruginosa Sodium Chloride Strains trypticase-soy broth
Throughout the period from October 2016 to March 2018, a total of 332 bacterial cultures were recovered from Qasr El-Aini, October 6 University Hospital, Dar El Salam Cancer Center, Al-Helal hospital, National Cardiology Institute, EL-Nile Badrawy hospital, Abu Al-Reish hospital, Sheikh-Zayed Specialized hospital, National Cancer Institute, and Medical Research Institute-Alexandria, Egypt. These bacterial isolates were recovered from different clinical specimens of hospitalized patients after obtaining informed consents. Clinical specimens were collected from the wound, burn, urine, and sputum. Environmental samples were collected from samples of water and fomites in hospital settings.
Fresh samples were cultivated on Cetrimide agar media, after which isolated species were identified by a regular microbiological technique such as colony morphology (green exopigment Cetrimide agar), Gram staining (Gram-negative rods), and biochemical reactions (oxidase-positive and citrate-positive). Identification at the species level was performed by using the Microbact™ Gram-negative system. The Microbact system was implemented in compliance with the manufacturer’s protocol (Oxoid, UK). Pseudomonas aeruginosa (ATCC 12924) standard strain was used as a positive control.
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Publication 2020
Agar Bacteria Cardiovascular System Cetrimide Citrate Fomites Malignant Neoplasms Microbiological Techniques Oxidases Patients Protocol Compliance Pseudomonas aeruginosa Rod Photoreceptors Sputum Strains Urine Wounds
For strain maintenance and cloning experiments, the strains P. putida KT2440 (DSM6125, ATCC47054), E. coli DH5α (New England Biolabs, Ipswich, MA, USA), and E. coli PIR2 (ThermoFisher Scientific, Waltham, MA, USA) were routinely cultivated in LB medium containing 10 g L−1 peptone, 5 g L−1 yeast extract, and 10 g L−1 NaCl. P. putida was cultivated at 30°C and E. coli at 37°C. If required, 50 μg mL−1 kanamycin or 30 μg mL−1 gentamycin were added to the medium to avoid loss of plasmid. After mating procedures, P. putida strains were selected on cetrimide agar (Sigma-Aldrich, St. Louis, MO, USA). Growth and production experiments were performed using M9 minimal medium with a final composition (per L) of 8.5 g Na2HPO4x2H2O, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 2 mM MgSO4, 4.87 mg FeSO4x7H2O, 4.12 mg CaCl2x2H2O, 1.5 mg MnCl2x4H2O, 1.87 mg ZnSO4x7H2O, 0.3 mg H3BO3, 0.25 mg Na2MoO4x2H2O, 0.15 mg CuCl2x2H2O, 0.84 mg Na2EDTAx2H2O (Sambrook and Russell, 2001 ), and 10 g glucose for pre-cultures or 10 g xylose for main cultures. Growth experiments were performed in 500 mL shake flasks with 10% filling volume at 200 rpm and in 24-deep well-plates (SystemDuetz; Enzyscreen B.V., Heemstede, The Netherlands) with 1 mL filling volume at 300 rpm.
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Publication 2019
Agar Cetrimide Escherichia coli Gentamicin Glucose Kanamycin Peptones Plasmids Sodium Chloride Strains Sulfate, Magnesium Tremor Xylose Yeast, Dried
The bacterial strains used in in vitro experiments included strains obtained from American Type Culture Collection (ATCC), and wild-type strains isolated from hospital surfaces. ATCC strains included S. aureus (ATCC 25923), E. coli (ATCC 25922), and P. aeruginosa (ATCC BAA-47). Hospital isolates included three strains selected for their drug resistance characteristics: S. aureus (SA2-R73), E. coli (EC-R60), and P. aeruginosa (PA-V6). Hospital isolates were collected by direct sampling of hospital surfaces with 55 mm diameter contact Rodac plates (24 cm2 surface), containing the following selective media: Baird–Parker agar (for Staphylococcus spp., cat. n. 146189), MacConkey agar (for Enterobacteriaceae spp., cat. n. 146427), and cetrimide agar (for Pseudomonas spp., cat. n. 146768) (all bacterial media were from Merck Millipore, Billerica, MA, USA). Grown colonies with morphological S. aureus, E. coli, and P. aeruginosa features were further streaked on the corresponding selective medium and incubated at 37°C for 24 hours, to isolate single pure colonies. Each individual colony was then characterized by Gram-staining, and identified by appropriate biochemical tests (API-Staph, cat. n. 20500, and API-20E, cat. n. 20100; Biomerieux, Florence, Italy).
After identification, all isolates were expanded overnight in tryptic soy broth (TSB, cat. n. 146599; Merck Millipore) at 37°C, frozen in 50% sterile glycerol, and kept at −80°C until use. Each isolate was characterized for antibiotic resistance by conventional disc-diffusion Kirby–Bauer antibiograms, using Mueller–Hinton agar plates (cat. n. 105437; Merck Millipore), testing the following antibiotics: penicillin G (cat. n. CT0043B; Oxoid, Altrincham, UK), ampicillin (Oxoid; cat. n. CT0003B; Oxoid), vancomycin (cat. n. CT0058B; Oxoid), oxacillin (cat. n. CT0040B; Oxoid), ofloxacin (cat. n. CT0446B; Oxoid), cefotaxime (cat. n. CT066B; Oxoid), cefoxitin (cat. n. CT0119B; Oxoid), gentamicin (cat. n. 9026; Liofilchem, Italy), imipenem (cat. n. CT0455B; Oxoid), aztreonam (cat. n. 9008; Liofilchem, Liofilchem, Teramo, Italy), meropenem (cat. n. 9068; Liofilchem), and colistin (cat. n. CT0017B; Oxoid). Zone inhibition diameters were interpreted according to the European Committee on Antimicrobial Susceptibility Testing breakpoint tables for interpretation of minimum inhibitory concentration (MIC) and inhibition zone diameters38 and to the Clinical and Laboratory Standards Institute manual (26th edition).39 In addition, MICs of resistant strains were also measured, accordingly to European Food Safety Authority guidelines, by using antibiotic stripes containing serial dilutions of each antibiotic (cat. n. 92003, 92033, 92006, 92066, 92141, 92009, 92054, 92085, 92099, 92015, 92102, 92057; Liofilchem).
The concentrated PCHS detergent included, as previously described,28 (link) 107/mL spores of three species of probiotics belonging to the Bacillus genus, namely Bacillus subtilis, Bacillus pumilus, and Bacillus megaterium.
Publication 2018
Agar Ampicillin Antibiogram Antibiotic Resistance, Microbial Antibiotics Antibiotics, Antitubercular Aztreonam Bacillus Bacillus megaterium Bacillus pumilus Bacillus subtilis Bacteria Cefotaxime Cefoxitin Cetrimide Clinical Laboratory Services Colistin Detergents Diffusion Enterobacteriaceae Escherichia coli Europeans Freezing Gentamicin Glycerin Imipenem Meropenem Microbicides Minimum Inhibitory Concentration Ofloxacin Oxacillin Penicillin G Probiotics Pseudomonas Pseudomonas aeruginosa Psychological Inhibition Resistance, Drug Spores Staphylococcal Infections Staphylococcus Staphylococcus aureus Sterility, Reproductive Strains Susceptibility, Disease Technique, Dilution tryptic soy broth Vancomycin
The following HAI-related microorganisms were monitored: Staphylococcus spp. and Staphylococcus aureus, Enterobacteriaceae, Acinetobacter, Pseudomonas spp., Clostridium difficile, Candida spp. and Aspergillus spp. A total of 360 microbiological samples were collected in duplicate (720 total samples). The following growth media were used: the Tryptic Soy Agar with Lecithin, Tween and Histidine (Merck Millipore, Darmstadt, Germany) general growth medium was used for total bacterial count; Baird Parker Agar (Merck Millipore, Darmstadt, Germany), moderately selective medium for coagulase-positive staphylococci; MacConkey Agar (Merck Millipore, Darmstadt, Germany), selective for Enterobacteriaceae; ChromaticTM Agar (Liofilchem®—Italy) for Acinetobacter species detection; Cetrimide Agar (Cetrimide agar base, BD Diagnostic Systems), selective for Pseudomonas spp.; Clostridium difficile Agar (Ref. 31044 Clostridium selective agar Lickson—Italy), selective for Clostridium difficile; Sabouraud Dextrose Contact Agar with chloramphenicol (Merck Millipore, Darmstadt, Germany), selective for Mycetes and Candida albicans. Incubation was performed aerobically at 37°C (48–72 hours) for Baird Parker, MacConkey, Cetrimide, ChromaticTM Agar and anaerobically Clostridium difficile Agar by using anaerobic jars (GasPak™, Thermo Fisher Scientific Inc.) with AnaerobicGenTM System (Thermo Fisher Scientific Inc.) at 37°C for 72 hours. Colony Forming Units (CFU) on all agar plates were manually counted after their respective incubation period. Identification of isolates was assessed by Staph System (Liofilchem—Italy) for Staphylococcus aureus, Entero Pluri Test (Liofilchem—Italy) for Escherichia coli, Oxi/Ferm Pluri Test (Liofilchem—Italy) for Pseudomonas aeruginosa and API 20 C Aux (bioMérieux, Inc) for Candida albicans. After incubation under anaerobic checking and detection of the Clostridium difficile was assessed by Latex Agglutination test (Liofilchem—Italy).
Bacillus isolates were obtained from Tryptic Soy Agar plates. At least 20 isolates per each sampling time were inoculated in 5 ml LB and expanded for 24 hours at 37°C for subsequent analyses.
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Publication 2016
Acinetobacter Agar Aspergillus Bacillus Candida Candida albicans Cetrimide Chloramphenicol Clostridium Clostridium difficile Coagulase Counts, Bacterial Diagnosis Enterobacteriaceae Escherichia coli Female Pseudohermaphroditism Glucose Histidine Latex Fixation Tests Lecithin Pseudomonas Pseudomonas aeruginosa Staphylococcal Infections Staphylococcus Staphylococcus aureus Trypsin Tweens

Most recents protocols related to «Cetrimide»

The present study provided the disks and powdered antibiotics from the MAST (Mast Diagnostics, United Kingdom) and Sigma-Aldrich (Taufkirchen, Germany). The following items were acquired from Merck (Merck, United States): Blood Agar, Mannitol Salt Agar (MSA), MacConkey agar, Cetrimide agar, Mueller Hinton Agar (MHA), DNA Agar, Mueller Hinton Broth (MHB), Trypticase Soy Broth (TSB), NaCl, glucose, and MgCl2. Fetal bovine serum (FBS), Dulbecco’s Modified Eagle’s Medium (DMEM), Fetal-Calf Serum (FCS), 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2 H-tetrazoliumbromide (MTT), Triton X-100, dimethyl sulfoxide (DMSO), agarose, ethanol, methanol, and crystal violet were provided from Sigma-Aldrich (Saint Louis, MO, United States). 96-well microplates, including flat-and round-bottom, were supplied by Jet Biofil (Guangzhou, China) and NEST Biotechnology (Wuxi, China), respectively.
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Publication 2023
Agar Antibiotics, Antitubercular BLOOD Cetrimide Diagnosis diphenyl Eagle Ethanol Fetal Bovine Serum Glucose Magnesium Chloride Mannitol Methanol Sepharose Sodium Chloride Sodium Chloride, Dietary Sulfoxide, Dimethyl Triton X-100 trypticase-soy broth Violet, Gentian
Biofilm assays were based on previously described methodology [13 (link)], but scaled up to six-well plates. In brief, overnight cultures of C. albicans and P. aeruginosa were washed in phosphate-buffered saline (PBS), and C. albicans resuspended at 1×106 cells ml−1 and P. aeruginosa to an OD600 of 0.2 [~2×108 colony-forming units (c.f.u.) ml−1] in Mueller–Hinton broth. Each well contained 3 ml C. albicans and 300 µl of P. aeruginosa in a total of 6 ml. Plates were incubated at 37 °C for 2 h to allow cells to adhere, at which point the media were replaced with fresh sterile media, and plates were incubated statically at 37 °C for 24 h. Cells not part of the biofilm were removed, media were replaced with fresh MHB containing 0 or 5 µg ml−1 meropenem, and plates were incubated for 4 h. Media were replaced with 2 ml PBS containing 50 µg ml−1 DNase I and plates were incubated at 37 °C for 1 h to degrade the extracellular matrix. Biofilms were detached from the plate by scraping, serially diluted and plated onto selective agar (YPD agar supplemented with 100 µg ml−1 tetracycline to determine viable C. albicans c.f.u. and cetrimide agar to determine viable P. aeruginosa c.f.u.).
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Publication 2023
Agar Biofilms Biological Assay Candida albicans Cells Cetrimide Deoxyribonuclease I Extracellular Matrix Meropenem Phosphates Pseudomonas aeruginosa Saline Solution Sterility, Reproductive Tetracycline
Biofilms were formed as described above and triplicate biofilms were pooled, 50 µl serially diluted and plated on cetrimide agar or YPD supplemented with 100 µg ml−1 tetracycline to check for contamination. Remaining biofilm cells were centrifuged at 3500 r.p.m at 4 °C for 5 min and pellets snap frozen in liquid nitrogen. Four biological replicates were shipped to GeneWiz, UK, for RNA extraction sequencing and basic bioinformatic analysis.
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Publication 2023
Agar Biofilms Biopharmaceuticals Cells Cetrimide Freezing Nitrogen Pellets, Drug Tetracycline

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Publication 2023
acetonitrile BaseLine dental cement Cetrimide High-Performance Liquid Chromatographies Methanol

Nebivolol HCl (NEB, 99.4% purity), was kindly supplied by Sigma Company for Pharmaceutical Industries, Quesna, Egypt.

Telmisartan (TEL,99.91% purity) was gently given by International Drug Agency for Pharmaceutical Industry (IDI), Cairo, Egypt

Pharmaceutical Preparations

Nevilob Tablets (Batch No. 2033518) labeled to include 5 mg NEB per tablet, a product of Marcyrl Pharmaceutical Industries, Cairo, Egypt.

Micardis Tablets (Batch No. 906949) are labeled to include 40 mg TEL per tablet, a product of Boehringer Ingelheim Pharma, Germany.

Both preparations were purchased from a local Pharmacy in the Egyptian market.

Methanol, acetonitrile, ethanol, Phenol, hexane, Tween-80, 99% cetrimide, 95% sodium dodecyl sulphate, methylcellulose, and β-cyclodextrin were bought from Sigma-Aldrich, Germany. All surfactants were prepared in distilled water at a concentration of 1% w/v or v/v.

Also, 96%acetic acid, sodium acetate trihydrate, sodium hydroxide, and boric acid were bought from the same source.

Acetate buffer (0.2 M) was prepared using sodium acetate trihydrate and acetic acid and its pH was adjusted at 3.7 − 5.5, while borate buffer (0.2 M) was composed of boric acid and potassium chloride, and pH was adjusted to cover the range of (6–9) using sodium hydroxide.

Human plasma samples were obtained from Blood Bank, Mansoura University Hospital (Mansoura, Egypt), and kept frozen at − 20 °C until use after gentle thawing.

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Publication 2023
Acetate Acetic Acid acetonitrile Borates boric acid Buffers Cetrimide Cyclodextrins Ethanol Freezing Hexanes Homo sapiens Methanol Methylcellulose Micardis Nebivolol Pharmaceutical Preparations Phenol Plasma Potassium Chloride Sodium Acetate Trihydrate Sodium Hydroxide Sulfate, Sodium Dodecyl Surfactants Tablet Telmisartan Tween 80

Top products related to «Cetrimide»

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Cetrimide agar is a microbiological culture medium used for the selective isolation and identification of Pseudomonas aeruginosa bacteria. It contains cetrimide, a quaternary ammonium compound that inhibits the growth of most other bacteria, allowing Pseudomonas aeruginosa to grow and be easily identified.
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Cetrimide agar is a microbiological culture medium used for the selective isolation and identification of Pseudomonas aeruginosa. It contains cetrimide, a cationic detergent, which inhibits the growth of most other bacteria, allowing for the selective growth of Pseudomonas species.
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Pseudomonas cetrimide agar is a selective and differential culture medium used for the isolation and identification of Pseudomonas aeruginosa in clinical and environmental samples. The medium contains cetrimide, which inhibits the growth of many gram-positive and gram-negative bacteria, allowing Pseudomonas aeruginosa to grow selectively. The medium also contains substances that promote the production of pyocyanin, a characteristic pigment produced by Pseudomonas aeruginosa, which aids in the identification of the organism.
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Cetrimide is a chemical compound commonly used as an active ingredient in various laboratory and medical products. It functions as a cationic surfactant, exhibiting antiseptic and disinfectant properties. Cetrimide is widely utilized in a range of applications, including as a component in disinfectants, antimicrobial agents, and other specialized laboratory equipment.
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MacConkey agar is a selective and differential culture medium used for the isolation and identification of Gram-negative enteric bacteria, particularly members of the Enterobacteriaceae family. It inhibits the growth of Gram-positive bacteria while allowing the growth of Gram-negative bacteria.
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Tween 80 is a non-ionic surfactant and emulsifier. It is a viscous, yellow liquid that is commonly used in laboratory settings to solubilize and stabilize various compounds and formulations.
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Mannitol salt agar is a microbiological growth medium used for the selective isolation and identification of Staphylococcus species. It contains mannitol as the carbohydrate source and high concentrations of sodium chloride, which inhibit the growth of many non-Staphylococcus bacterial species.
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Cetrimide agar is a selective and differential microbiology culture medium used for the isolation and identification of Pseudomonas aeruginosa. It contains cetrimide, which inhibits the growth of most other bacteria, allowing for the selective growth of Pseudomonas species.
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Phosphoric acid is a chemical compound with the formula H3PO4. It is a colorless, odorless, and viscous liquid that is commonly used in various industrial and laboratory applications.
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Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.

More about "Cetrimide"

Cetrimide, also known as cetyltrimethylammonium bromide (CTAB), is a quaternary ammonium compound that serves as a versatile antimicrobial agent and surfactant.
This broad-spectrum compound exhibits potent activity against a wide range of bacteria, fungi, and viruses, making it a valuable tool in various applications.
Cetrimide agar is a selective medium used for the isolation and identification of Pseudomonas aeruginosa, a common opportunistic pathogen.
Pseudomonas cetrimide agar, a variation of this medium, is also employed for the same purpose.
These agar-based substrates leverage the antimicrobial properties of cetrimide to inhibit the growth of other microorganisms, allowing for the selective cultivation of Pseudomonas species.
In addition to its antimicrobial function, cetrimide is also utilized as a surfactant in personal care products, pharmaceutical formulations, and disinfection procedures.
Its ability to reduce surface tension and facilitate the penetration of active ingredients makes it a valuable excipient in various applications.
Tween 80, another commonly used surfactant, is often combined with cetrimide to enhance its effectiveness.
Mannitol salt agar, a selective medium for the isolation of Staphylococcus species, may also be used in conjunction with cetrimide-based media to provide a more comprehensive microbial analysis.
The chemical structure of cetrimide, which includes a quaternary ammonium group and a long alkyl chain, contributes to its surfactant properties and antimicrobial mechanisms.
Phosphoric acid and acetonitrile are occasionally employed in analytical procedures involving cetrimide, such as chromatographic techniques, to facilitate separation and detection.
By harnessing the power of AI-driven comparisons, the PubCompare.ai platform can assist researchers in optimizing their cetrimide-related studies.
This tool helps identify the best protocols and products from the available literature, preprints, and patents, thereby enhancing the reproducibility and accuracy of cetrimide research.
With the insights provided by PubCompare.ai, researchers can discover the optimal cetrimide protocols and maximize the impact of their work.