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CH 223191

CH 223191 is a chemical compound with potential applications in biomedical research and development.
This substance has been the subject of scientific investigations, with researchers exploring its properties and potential uses.
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Most cited protocols related to «CH 223191»

Naive CD4 T cells were isolated by FACS sorting using a MoFlo sorter of lymph nodes cell suspensions for CD44lo CD25 CD4+ cells. The culture mediums used were IMDM (Sigma-Aldrich) or RPMI 1640 (Sigma-Aldrich), both supplemented with 2 × 10−3 M l-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, 5 × 10−5 M β-mercaptoethanol, and 5% FCS. In some cases, RPMI medium was supplemented with 11 mg/liter l-tryptophan (Invitrogen) to adjust it to the concentrations found in IMDM.
Th17 cells were differentiated on plates coated with 2 μg/ml anti-CD3 + 5 μg/ml anti-CD28 with a cytokine cocktail of 50 ng/ml IL-6, 1 ng/ml TGF-β, and 10 ng/ml IL-1. Neutralizing antibodies to IFN-γ, IL-4, or IL-2 were added at 10 μg/ml in some experiments. Th1 cells were stimulated in the presence of 3 ng/ml IL-12, and iT reg cells were generated by adding 10 ng/ml TGF-β. The AhR antagonist CH-223191 (Calbiochem) was added at 3 μM at the start of culture. FICZ was added at 300 nM at the start of some cultures. For measurements of intracellular cytokines, T cells were restimulated with 500 ng/ml phorbol dibutyrate and 500 ng/ml ionomycin in the presence of brefeldin A for 4 h on day 5 after initiation of cultures. Measurement of Stat5 phosphorylation was done with antibodies to pStat5 (BD) according to the manufacturer's instructions.
Publication 2009
2-Mercaptoethanol 6-formylindolo(3,2-b)carbazole Antibodies Antibodies, Neutralizing Brefeldin A CD4 Positive T Lymphocytes Cells CH 223191 Cytokine Glutamine IL2RA protein, human Interferon Type II Interleukin-12 Ionomycin Lymphocyte Muromonab-CD3 Nodes, Lymph Penicillins phorbol Phosphorylation Protoplasm STAT5A protein, human Streptomycin T-Lymphocyte Th17 Cells Transforming Growth Factor beta Tryptophan Type 1 Helper T Cells
To induce colitis, mice were administered drinking water supplemented with 2% (wt./vol.) dextran sulfate sodium (DSS; MP Biomedicals, LLC, Aurora, OH, USA) for 7 d and were then allowed to recover by drinking unsupplemented water for the next 5 d (Supplementary Fig. 1a). The 6-formylindolo(3,2-b)carbazole (Ficz; Enzo Life Sciences, Lausen, Switzerland) and the AHR antagonist CH223191 (AHR; Sigma-Aldrich) were resuspended in dimethyl sulfoxide (DMSO; Sigma-Aldrich) and administered intraperitoneally. Ficz was injected 1 d after DSS administration (1 μg/mouse). For the AHR treatment, WT→GF and Card9−/−→GF mice (4- to 5-week-old females) were treated (100 μg/mouse) three times per week until euthanization (Fig. 5c). Controls consisted of mice injected with DMSO vehicle alone for the Ficz and AHR treatment groups. Three bacteria with strong AHR activity and that were isolated in feces of WT mice were identified by sequencing the 16S rDNA gene as previously described36 (link). The resulting sequences were aligned, inspected by eye, and compared with the online tool BLAST. Strains were identified based on the highest hit scores. These strains were deposited in the Collection Nationale de Cultures de Microorganismes (CNCM) of the Institut Pasteur and named L. murinus CNCM I-5020, L. reuteri CNCM I-5022, and L. taiwanensis CNCM I-5019. Bacterial suspensions containing these three strains (109 colony-forming units (c.f.u.) of each strain in 500 μl of PBS) were administered three times per week for a period of 3 weeks to WT→GF and Card9−/−→GF mice (4- to 5-week-old females) by intragastric gavage (Fig. 5c). Oral gavage with PBS was performed in control mice. For the antifungal treatment, mice were fed 0.5 mg/ml fluconazole in drinking water (Sigma-Aldrich) 1 week before DSS administration and every day thereafter, as previously described19 (link) (Supplementary Fig. 6c). For the IL-22 treatment, WT and Card9−/− mice were injected intraperitoneally three times per week with mouse IL-22–Fc (50 μg/mouse) (Genentech, South San Francisco, CA, USA) (WT IL-22 and Card9−/− IL-22) or an equivalent amount of isotype control (IgG2a) (Genentech) (WT isotype and Card9−/− isotype) for a period of 3 weeks. 3 d after the last injections, colitis was induced by DSS treatment (Supplementary Fig. 13c). In all treatments, body weight, blood in stool, and stool consistency were analyzed daily. The severity of colitis was assessed using the disease activity index (DAI) as previously described8 (link).
Publication 2016
6-formylindolo(3,2-b)carbazole Antifungal Agents Bacteria BLOOD Body Weight carbazole CARD9 protein, human CH 223191 Colitis Dextran Sulfate Sodium Feces Females Fluconazole Genes IgG2A IL22 protein, human Immunoglobulin Isotypes Lactobacillus reuteri Mus NG-Nitroarginine Methyl Ester Recombinant DNA Strains Sulfoxide, Dimethyl Tube Feeding
HEK293 cells were grown in DMEM supplemented with 10% FBS and transfected with Fugene-HD Transfection Reagent (Roche), reporter constructs (pAhR-Luc/pTK-Renilla or pGud-Luc/pTK Renilla) as well transcription factor expression constructs (pStat1, pIrf9) as indicated. In some experiments, transfected cells were exposed to 500 IU/ml of mIFN-β (Bio X cell) or AhR inhibitor CH223191 (Sigma). For the measurement of AhR ligands in human serum, 15.000 HEK293 cells were plated in 96 well plates 24 h before transfection with pGud-Luc and pTK-Renilla. After 24 h transfected cells were incubated with DMEM supplemented with 10% of patient serum. Luciferase activity was analyzed 24 h later using Dual Luciferase Reporter System (Promega) and normalized to Renilla luciferase activity.
Publication 2016
Cells CH 223191 FuGene HEK293 Cells Homo sapiens Ligands Luciferases Luciferases, Renilla Patients Promega Sea Pansy Serum Transcription Factor Transfection
HEK293 cells were grown in DMEM supplemented with 10% FBS and transfected with Fugene-HD Transfection Reagent (Roche), reporter constructs (pAhR-Luc/pTK-Renilla or pGud-Luc/pTK Renilla) as well transcription factor expression constructs (pStat1, pIrf9) as indicated. In some experiments, transfected cells were exposed to 500 IU/ml of mIFN-β (Bio X cell) or AhR inhibitor CH223191 (Sigma). For the measurement of AhR ligands in human serum, 15.000 HEK293 cells were plated in 96 well plates 24 h before transfection with pGud-Luc and pTK-Renilla. After 24 h transfected cells were incubated with DMEM supplemented with 10% of patient serum. Luciferase activity was analyzed 24 h later using Dual Luciferase Reporter System (Promega) and normalized to Renilla luciferase activity.
Publication 2016
Cells CH 223191 FuGene HEK293 Cells Homo sapiens Ligands Luciferases Luciferases, Renilla Patients Promega Sea Pansy Serum Transcription Factor Transfection
Monolayers of A72, pretreated or not with CH223191, were infected or not with CCoV, at an MOI of 0.05 and 5, incubated at 37 °C, and processed after 24 h of infection by real-time PCR for CCoV quantification. Furthermore, viral cytopathic effect (CPE) was evaluated, by examining cells under a light microscope at 24 h p.i. [21 (link),27 (link)].
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Publication 2022
Cells CH 223191 Cytopathogenic Effect, Viral Infection Light Microscopy Real-Time Polymerase Chain Reaction

Most recents protocols related to «CH 223191»

L-Kynurenine (L-KYN), CH-223191, ferrostatin-1 (Fer-1), necrostatin-1 (Nec-1), Z-VAD-FMK (Z-VAD), VX-765 and GPX4-IN-3 were all purchased from MedChemExpress. We conducted preliminary experiments to determine the effective concentration and safety of each inhibitor on NK cells. For the culture supernatant treatment assays, the GES-1 or SGC-7901 cells were changed to be cultured with NK-92 complete culture medium for 24 h, which contained the highest concentration of L-KYN as determined in Fig. 1A. The supernatants were collected and mixed with 20% fresh medium, which were then used to treat NK cells for 24 or 48 h.

IDO produced L-KYN from GC cells impairs NK viability in vitro. A The concentration of L-KYN in the culture supernatant of SGC-7901, MGC-803 or GES-1 cells measured by ELISA. B The ratio of the remaining NK-92 cell number relative to the originally seeded cell number in the non-contract co-culture system with GES-1, MGC-803 or SGC-7901 cells. The control group referred to that the NK-92 cells were seeded into the co-culture wells alone. C The flow cytometric analysis for the proportion of FVD+ NK-92 cells in the co-culture system. The pictures above showed the representative results. D The flow cytometric results that showed the proportion of FVD+ NK-92 cells when stimulated with the culture supernatant of GES-1, MGC-803 or SGC-7901 cells. The control group referred to the medium without culturing with cells. E The proportion of FVD+ NK-92 cells when treated with gradient concentrations of L-KYN for 24 or 48 h. F and G The proportion of FVD+ NK-92 cells when co-cultured with GES-1, SGC-7901Con, SGC-7901IDO-KO (F), or treated with the culture supernatant of SGC-7901Con or SGC-7901IDO-KO (G) for 48 h. The control group in (F) indicated the NK-92 cells were only treated with medium. H and I The proportion of FVD+ primary human NK (hNK) cells when treated with gradient concentrations of L-KYN for 48 h (H) or the culture supernatant from GES-1, SGC-7901Con and SGC-7901IDO-KO cells (I). All the results were replicated in 3 (H and I) or 4 (A - G) independent experiments. * refers to the p-value of group SGC-7901 vs GES-1 and # refers to the group SGC-7901 vs MGC-803 (A - D). * p < 0.05, ** p < 0.01, *** p < 0.001, # p < 0.05, ## p < 0.01, ### p < 0.001

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Publication 2023
benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone Biological Assay Cells CH 223191 Enzyme-Linked Immunosorbent Assay ferrostatin-1 Flow Cytometry Homo sapiens Kynurenine L Cells Natural Killer Cells necrostatin-1 Phospholipid Hydroperoxide Glutathione Peroxidase Safety VX-765
Vero and Huh-7 cell monolayers were pretreated with CH223191 and kynurenine diluted in MEM 1.5% NBCS for 1 h or 24 h, respectively, at 37 °C. CH223191 was used at concentrations ranging between 1.25 and 20 µM, while kynurenine was used at concentrations ranging between 5 and 80 µM. Then, viral infection was performed with either the IV4454 or Candid#1 strain at an MOI of 0.5. After 1 h, the inocula were removed and the cells were exposed to both compounds for 48 h at the mentioned concentrations. At 48 h pi supernatants were collected for plaque assay and cell monolayers were processed for either indirect immunofluorescence or RT-qPCR.
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Publication 2023
Biological Assay Cells CH 223191 Dental Plaque Indirect Immunofluorescence Kynurenine Strains Virus Diseases
The synthetic AHR-specific competitive antagonist CH223191, 1-Methyl-N-[2-methyl-4-[2-(2-methylphenyl)diazenyl]phenyl]-1H-pyrazole-5-carboxamide (Tocris Bioscience, Bristol, United Kingdom), was solubilized in dimethyl sulfoxide (DMSO) and used at a concentration of 1.25, 2.5, 5, 10, 20, 40, and 80 µM.
The tryptophan metabolite L-kynurenine, (2S)-2-Amino-4-(2-aminophenyl)-4-oxo-butanoic acid (Sigma-Aldrich, St. Louis, MO, USA) was solubilized in DMSO and used at a concentration of 2.5, 5, 10, 20, 40, 80, 160, and 320 µM.
All treatments were performed in the absence of light, given that the compounds present photosensitivity.
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Publication 2023
Butyric Acid CH 223191 Kynurenine Light Photosensitization pyrazole-5-carboxamide Sulfoxide, Dimethyl Tryptophan
Huh-7 monolayers treated with CH223191 or kynurenine and infected with JUNV as mentioned above were fixed at 48 h pi with methanol for 10 min at −20 °C, washed thrice with PBS, and stored in fresh PBS at 4 °C until processing. The following antibodies were used: primary mouse monoclonal anti-NP antibodies (1:200) (NA05-AG12) [21 (link)] and secondary Alexa Fluor 488 conjugated anti-mouse goat antibodies (1:200) (A-11001) (ThermoFisher Scientific, Waltham, MA, USA). Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (1:1000) (Sigma-Aldrich, St. Louis, MO, USA). Microscopy and photography data were obtained using an Olympus IX71 fluorescence microscope with a QImaging Exi-Aqua camera attached (pixel size: 6.45 μm; photodetector area: 1392 × 1040 pixels2). Quantification of fluorescence data was performed using Fiji software (version 1.53v) (National Institutes of Health, Bethesda, MD, USA). Manual quantifications of DAPI-stained nucleus and NP-positive cells were performed manually through the cell counter plugin included in the Fiji software (version 1.53v). Cells were considered positive if NP viral antigen staining could be detected. Percentage of NP-positive cells was expressed as the ratio between NP-positive cells and total cells in each field and then the average percentage was determined and presented. Fluorescence intensity of NP-positive cells was obtained by measuring mean gray value of individual cells and subtracting background fluorescence. Viral foci were delimited by considering groups of NP-positive cells surrounded by NP-negative cells. Viral foci were counted on 3 different images from 3 different randomly chosen fields and the average foci size was determined by the number of cells that formed them.
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Publication 2023
alexa fluor 488 Anti-Antibodies Antibodies Antigens, Viral Cell Nucleus Cells CH 223191 Fluorescence Goat Kynurenine Methanol Microscopy Microscopy, Fluorescence Mus
For cell viability determination Huh-7 and Vero cell monolayers were grown in 96 well plates for 24 h in MEM 5% NBCS. Cell monolayers at 90% confluency were treated with different concentrations of CH223191 (1.25 µM to 80 µM) and kynurenine (5 µM to 320 µM) for 72 h. Mock was treated with the same concentration of DMSO as the highest dose of ligand treatment and was considered as 100% of cell viability. All treatments were performed in quadruplicate. Cell viability was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) (Sigma-Aldrich, St. Louis, MO, USA) assay. Absorbance was measured at 570 nm in a FLUOstar OPTIMA microplate reader. Cell viability % was determined by relativizing treatment absorbance results using Mock absorbance values.
Additionally, growth capability and morphology of treated cells were studied under light microscope. Pictures were taken at 72 h with a Nikon Eclipse TS100 microscope; magnification: 100×; NA: 0.25.
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Publication 2023
Biological Assay Bromides Cells Cell Survival CH 223191 diphenyl Kynurenine Ligands Light Microscopy Microscopy Sulfoxide, Dimethyl Tetrazolium Salts Vero Cells

Top products related to «CH 223191»

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CH223191 is a laboratory equipment product. It is used for scientific research and analysis purposes. The core function of this product is to facilitate the measurement and analysis of various samples and materials in a laboratory setting. However, a detailed description of its specific features and capabilities cannot be provided while maintaining an unbiased and factual approach.
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
Sourced in United Kingdom
CH223191 is a laboratory equipment product offered by Bio-Techne. It is a key component used for specific scientific applications, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Sourced in China, United States
CH-223191 is a laboratory instrument used for the quantitative analysis of chemical compounds. It is designed to measure the concentration of specific substances in a sample through spectroscopic methods. The core function of this equipment is to provide accurate and reliable data for research, quality control, or other analytical applications.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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CH223191 is a laboratory reagent. It is a small organic compound used for chemical synthesis and analysis in research settings.
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Benzo[a]pyrene is a polycyclic aromatic hydrocarbon commonly used as a reference compound in various laboratory applications. It serves as a standard for analytical techniques and is often employed in research, environmental monitoring, and regulatory compliance testing.
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Kynurenine is a laboratory product manufactured by Merck Group. It is a biochemical compound used in various research and analytical applications. Kynurenine serves as a key intermediate in the kynurenine pathway, a metabolic process involving the degradation of the amino acid tryptophan.
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
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RPMI 1640 is a common cell culture medium used for the in vitro cultivation of a variety of cells, including human and animal cells. It provides a balanced salt solution and a source of essential nutrients and growth factors to support cell growth and proliferation.

More about "CH 223191"

Discover the versatile compound CH 223191, a chemical with vast potential in biomedical research and development.
This substance, also known as CH-223191 or FBS, has been the subject of extensive scientific investigations, with researchers exploring its unique properties and diverse applications.
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Beyond its research applications, CH 223191 shares similarities with other compounds like DMSO and Benzo[a]pyrene, which are also widely used in scientific investigations.
Additionally, the inclusion of Kynurenine and Penicillin/streptomycin in research protocols can provide valuable insights and enhance experimental outcomes.
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