Th17 cells were differentiated on plates coated with 2 μg/ml anti-CD3 + 5 μg/ml anti-CD28 with a cytokine cocktail of 50 ng/ml IL-6, 1 ng/ml TGF-β, and 10 ng/ml IL-1. Neutralizing antibodies to IFN-γ, IL-4, or IL-2 were added at 10 μg/ml in some experiments. Th1 cells were stimulated in the presence of 3 ng/ml IL-12, and iT reg cells were generated by adding 10 ng/ml TGF-β. The AhR antagonist CH-223191 (Calbiochem) was added at 3 μM at the start of culture. FICZ was added at 300 nM at the start of some cultures. For measurements of intracellular cytokines, T cells were restimulated with 500 ng/ml phorbol dibutyrate and 500 ng/ml ionomycin in the presence of brefeldin A for 4 h on day 5 after initiation of cultures. Measurement of Stat5 phosphorylation was done with antibodies to pStat5 (BD) according to the manufacturer's instructions.
CH 223191
This substance has been the subject of scientific investigations, with researchers exploring its properties and potential uses.
The page description outlines an innovative platform, PubCompare.ai, that enhances reproducibility and accuracy in research protocols by leveraging AI-driven comparisons to locate relevant protocols from literature, pre-prints, and patents.
Researchers can use this tool to identify the best protocols and products, empowering their research journey and experiecing the future of protocol enhancement.
Most cited protocols related to «CH 223191»
Th17 cells were differentiated on plates coated with 2 μg/ml anti-CD3 + 5 μg/ml anti-CD28 with a cytokine cocktail of 50 ng/ml IL-6, 1 ng/ml TGF-β, and 10 ng/ml IL-1. Neutralizing antibodies to IFN-γ, IL-4, or IL-2 were added at 10 μg/ml in some experiments. Th1 cells were stimulated in the presence of 3 ng/ml IL-12, and iT reg cells were generated by adding 10 ng/ml TGF-β. The AhR antagonist CH-223191 (Calbiochem) was added at 3 μM at the start of culture. FICZ was added at 300 nM at the start of some cultures. For measurements of intracellular cytokines, T cells were restimulated with 500 ng/ml phorbol dibutyrate and 500 ng/ml ionomycin in the presence of brefeldin A for 4 h on day 5 after initiation of cultures. Measurement of Stat5 phosphorylation was done with antibodies to pStat5 (BD) according to the manufacturer's instructions.
Most recents protocols related to «CH 223191»
IDO produced L-KYN from GC cells impairs NK viability in vitro.
The tryptophan metabolite L-kynurenine, (2S)-2-Amino-4-(2-aminophenyl)-4-oxo-butanoic acid (Sigma-Aldrich, St. Louis, MO, USA) was solubilized in DMSO and used at a concentration of 2.5, 5, 10, 20, 40, 80, 160, and 320 µM.
All treatments were performed in the absence of light, given that the compounds present photosensitivity.
Additionally, growth capability and morphology of treated cells were studied under light microscope. Pictures were taken at 72 h with a Nikon Eclipse TS100 microscope; magnification: 100×; NA: 0.25.
Top products related to «CH 223191»
More about "CH 223191"
This substance, also known as CH-223191 or FBS, has been the subject of extensive scientific investigations, with researchers exploring its unique properties and diverse applications.
One innovative platform, PubCompare.ai, is revolutionizing the way researchers approach protocol enhancement.
Leveraging the power of AI-driven comparisons, this tool empowers researchers to effortlessly locate relevant protocols from literature, pre-prints, and patents.
By identifying the best protocols and products, researchers can optimize their workflow and experiance the future of protocol enhancement.
Beyond its research applications, CH 223191 shares similarities with other compounds like DMSO and Benzo[a]pyrene, which are also widely used in scientific investigations.
Additionally, the inclusion of Kynurenine and Penicillin/streptomycin in research protocols can provide valuable insights and enhance experimental outcomes.
Explore the vast possibilities of CH 223191 and harness the power of innovative tools like PubCompare.ai to elevate your research journey.
Unlock new discoveries and experiance the future of protocol enhancement today.