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CL 316243

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Most cited protocols related to «CL 316243»

1
Adipocyte-specific CPT2 Knockout Mice
Cited 11 times

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Publication 2014
Acclimatization Adipocytes Adipoq protein, mouse Animals Animals, Laboratory Blood Glucose Body Temperature Body Weight Cholesterol CL 316243 Cold Temperature Cyclic AMP-Responsive DNA-Binding Protein Diet Diet, High-Fat Edetic Acid Embryonic Stem Cells Exons Fat-Restricted Diet Food FRAP1 protein, human Freezing Genes Glucose Glucose Tolerance Test Glycerin Hydroxybutyrates Immune Tolerance Injections, Intraperitoneal Insulin Introns Liver Mice, Laboratory Mice, Transgenic Muscle Tissue Nitrogen Nonesterified Fatty Acids Recombination, Genetic Rectum Rodent Serum Sucrose Tail Thermogenesis Thermometers Tissues Tromethamine
2
Cold Challenge Experiments on Mice
Cited 8 times
Male mice, 8-12 weeks old, were used in all experiments. Breeding pairs of wild type and STAT6−/− mice on BALB/cJ background were purchased from the Jackson Laboratory, and IL4/IL13−/−, IL4RαL/L, and LysMCre mice on the BALB/cJ background were obtained from the Locksley or Brombacher laboratories. For cold challenge experiments, mice were fed ad lib and individually housed in cages that had been pre-chilled at 4 °C8 (link). Core body temperature was monitored hourly by a rectal temperature probe (Physitemp). For the thermoneutrality experiments, mice were adapted to 30 °C in laboratory incubator (Darwin Chambers) for 2-4 weeks prior to experimentation. For rescue experiments, the β3-adrenergic agonist CL-316243 (Sigma) was injected intraperitoneally at 0.1mgkg−1 30 min before the cold challenge. Tissues were harvested at the end of 6 h cold challenge, and processed for RNA and protein analyses. To deplete macrophages, mice were injected intraperitoneally with two doses of clodronate-containing or empty liposomes (400 μl and 100 μl at 24 h and 30 min, respectively, prior to initiation of experiment)22 (link). Depletion was confirmed by flow cytometric analysis of monocytes and macrophages in blood, adipose tissues, and spleen. Cohorts of ≥ 4 mice per genotype or treatment were assembled for all in vivo studies, which were repeated 2-3 independent times. All data are presented as mean ± s.e.m.
Nguyen K.D., Qiu Y., Cui X., Goh Y.P., Mwangi J., David T., Mukundan L., Brombacher F., Locksley R.M, & Chawla A. (2011). Alternatively activated macrophages produce catecholamines to sustain adaptive thermogenesis. Nature, 480(7375), 104-108.
Publication 2011
Adrenergic Agonists BLOOD CL 316243 Clodronate Cold Temperature Flow Cytometry Genotype Interleukin-13 Liposomes Macrophage Males Monocytes Mus Proteins Rectum Spleen STAT6 protein, human Tissue, Adipose Tissues
3
Cold Challenge Experiments on Mice
Cited 8 times
Male mice, 8-12 weeks old, were used in all experiments. Breeding pairs of wild type and STAT6−/− mice on BALB/cJ background were purchased from the Jackson Laboratory, and IL4/IL13−/−, IL4RαL/L, and LysMCre mice on the BALB/cJ background were obtained from the Locksley or Brombacher laboratories. For cold challenge experiments, mice were fed ad lib and individually housed in cages that had been pre-chilled at 4 °C8 (link). Core body temperature was monitored hourly by a rectal temperature probe (Physitemp). For the thermoneutrality experiments, mice were adapted to 30 °C in laboratory incubator (Darwin Chambers) for 2-4 weeks prior to experimentation. For rescue experiments, the β3-adrenergic agonist CL-316243 (Sigma) was injected intraperitoneally at 0.1mgkg−1 30 min before the cold challenge. Tissues were harvested at the end of 6 h cold challenge, and processed for RNA and protein analyses. To deplete macrophages, mice were injected intraperitoneally with two doses of clodronate-containing or empty liposomes (400 μl and 100 μl at 24 h and 30 min, respectively, prior to initiation of experiment)22 (link). Depletion was confirmed by flow cytometric analysis of monocytes and macrophages in blood, adipose tissues, and spleen. Cohorts of ≥ 4 mice per genotype or treatment were assembled for all in vivo studies, which were repeated 2-3 independent times. All data are presented as mean ± s.e.m.
Nguyen K.D., Qiu Y., Cui X., Goh Y.P., Mwangi J., David T., Mukundan L., Brombacher F., Locksley R.M, & Chawla A. (2011). Alternatively activated macrophages produce catecholamines to sustain adaptive thermogenesis. Nature, 480(7375), 104-108.
Publication 2011
Adrenergic Agonists BLOOD CL 316243 Clodronate Cold Temperature Flow Cytometry Genotype Interleukin-13 Liposomes Macrophage Males Monocytes Mus Proteins Rectum Spleen STAT6 protein, human Tissue, Adipose Tissues
4
Ex Vivo BAT Lipolysis Assay
Cited 4 times
The lipolysis activity of BAT was measured, using ex vivo lipolysis assay as described [76 (link)]. Briefly, interscapular BAT was dissected and separated into two pieces. Each piece was put into culture medium {DMEM with 0.5% fatty acid free BSA (Sigma, St. Louis, MO)} and minced into tiny pieces with scissors. The tissues were incubated at 37°C with 10 μM CL316243 (Sigma, St. Louis, MO) as stimulated condition, or DMSO as basal condition. 2 hours later, medium was collected and heated at 85°C for 10 minutes. After spin down, clear supernatant was transferred to a new tube, and 10 μl was used to measure free glycerol content using Free Glycerol Reagent (Sigma, St. Louis, MO). Lipolysis activity was represented by glycerol concentrations, normalized by weight of the tissue.
Lin L., Lee J.H., Bongmba O.Y., Ma X., Zhu X., Sheikh-Hamad D, & Sun Y. (2014). The suppression of ghrelin signaling mitigates age-associated thermogenic impairment. Aging (Albany NY), 6(12), 1019-1032.
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Publication 2014
Biological Assay CL 316243 Fatty Acids Glycerin Lipolysis Sulfoxide, Dimethyl Tissues
5
Inducing Browning in White Fat
Cited 4 times
All animal experiments were performed according to procedures approved by UCSF’s Institutional Animal Care and Use Committee for animal care and handling. C57BL/6 mice (Jackson Laboratories) were used for the animal experiments. To induce the browning of white fat in mice, CL316243 at a dose of 1 mg/kg or saline control was injected intraperitoneally for 8 days into 6–8 week-old mice. Inguinal adipose tissue was isolated, fixed in 4% paraformaldehyde, and embedded in paraffin for histological analysis.
Sharp L.Z., Shinoda K., Ohno H., Scheel D.W., Tomoda E., Ruiz L., Hu H., Wang L., Pavlova Z., Gilsanz V, & Kajimura S. (2012). Human BAT Possesses Molecular Signatures That Resemble Beige/Brite Cells. PLoS ONE, 7(11), e49452.
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Publication 2012
Animals CL 316243 Groin Institutional Animal Care and Use Committees Mice, House Mice, Inbred C57BL Paraffin Embedding paraform Saline Solution Tissue, Adipose White Adipose Tissue

Most recents protocols related to «CL 316243»

1
Transcriptome Analysis of Murine Adipose Tissue
Reads were mapped to Ensembl mm10 release 92 using STAR (2.5.3a), and transcripts counted with FeatureCounts (1.6.0). Sequencing depth ranged from 54.2 to 78.6 million with a mean of 66.3 million reads. Transcriptomic data are deposited under accession number GSE199429. Reads were filtered using filterbyExpression, with a minimum count of 10 and design matrix modeling group. Differential gene expression analysis was performed using edgeR, quasi-likelihood negative binomial generalized log-linear model, and the design ~0 + group. Gene ontology (GO) enrichment analysis was performed utilizing clusterProfiler and molecular function GO terms, with all detected genes serving as background. Enrichment signatures of genes affected by isoprenaline (GSE43658) (77 (link)), dexamethasone (only 24-h treatments; GSE62635) (78 (link)), and CL316243 beta adrenergic stimulation (GSE55934) (79 (link)) was performed using fry. All P-values were adjusted using false discovery rate correction, and alpha was set to 0.05. Rhythmic oscillations in gene expression and glycerol release were analyzed with R package RAIN (Rhythmicity Analysis Incorporating Non-parametric Methods, release 3.15) (80 (link)), with period set to 24 h, method independent, and period delta set to 12 h. Outcomes with a P < 0.05 were considered as rhythmic.
Pendergrast L.A., Lundell L.S., Ehrlich A.M., Ashcroft S.P., Schönke M., Basse A.L., Krook A., Treebak J.T., Dollet L, & Zierath J.R. (2023). Time of day determines postexercise metabolism in mouse adipose tissue. Proceedings of the National Academy of Sciences of the United States of America, 120(8), e2218510120.
Publication 2023
Adrenergic Agents CL 316243 Dexamethasone Gene Expression Gene Expression Profiling Genes Glycerin Isoproterenol Rain
2
Metabolic Assessment of Ssu72 Knockout Mice
Metabolic studies were conducted at Soonchunhyang University under an approved SCH-IACUC protocol. Energy expenditure and associated experiments were measured using a Phenomaster (TSE systems) at Soonchunhyang Biomedical Research Core-facility of Korea Basic Science Institute (KBSI). Oxygen consumption (VO2) and CO2 release rates (VCO2) were measured every 24 min. Energy expenditure and Respiratory exchange rate (RER) were calculated by the ratio between VO2 and VCO2. Food intake was automatically monitored by Phenomaster food measurement module and locomotor activity was obtained by counting the number of the x-axis and y-aixs beam breaks. Ssu72 WT and aKO mice were injected intraperitoneally with a β3-adrenergic receptor-specific agonist CL-316,243 (Sigma-Aldrich, #5976) at a dose of 10 mg/kg to examine their responses to adrenergic stimulation, and VO2 was measured every 2 min.
Park E.J., Kim H.S., Lee D.H., Kim S.M., Yoon J.S., Lee J.M., Im S.J., Lee H., Lee M.W, & Lee C.W. (2023). Ssu72 phosphatase is essential for thermogenic adaptation by regulating cytosolic translation. Nature Communications, 14, 1097.
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Publication 2023
Adrenergic Agents Adrenergic Agonists CL 316243 Eating Energy Metabolism Epistropheus Food Institutional Animal Care and Use Committees Locomotion Mus Oxygen Consumption Respiratory Rate
3
Molecular Docking of CPEF Phenolics on UCP1 and PPARα
Molecular docking studies were conducted to further elucidate the phenolic compounds that might be responsible for the increased expression of UCP1 and PPARα in CPEF-treated db/db mice. Seven of the eight major phenolic polyphenols identified in CPEF were used for the molecular docking procedure. The flavanone, eriodictyol-O-deoxyhexose-O-hexose, was excluded due to the unknown position and the identities of the sugar moieties in the molecular structure. The 2-D structures of the compounds were then drawn using ChemDraw (Version 8.0, PerkinElmer Informatics, Waltham, MA, USA) and optimised using Avogadro (Version 1.2) [62 (link)], in which partial charges were added using the Ghemical force field, followed by energy minimisation to obtain the optimal geometry for each compound.
The 3-D models of Mus-musculus UCP1 and PPARα were modelled using the Swiss-Model online tool [63 (link)]. Both UCP1 and PPARα were homology modelled for this study due to the lack of Mus-musculus crystal structures. The template used to generate the UCP1 model was PDB ID 2LCK, whilst the templates used for the PPARα model were PDB ID’s 3DZY and 1K7L. Models were validated using the MolProbity online server [64 (link)]. Using UCSF Chimera (Version 1.15) [65 (link)], the protein structures were optimised for molecular docking by removing the water molecules and all non-standard residues. The molecular docking simulation was then carried out using Autodock Vina software Version 1.2.0 [66 (link)], with the docking grid box co-ordinates presented in Table 2. Based on the Autodock Vina scoring technique, the docked complexes were ranked on binding affinity score and the root mean square deviation from the original structural pose of each compound. All complexes were superimposed to ensure the validation of the docking procedure and binding site (Figure S1). Following the molecular docking procedure, the predicted intermolecular interactions, stabilising the compound within the active site of each protein, were assessed using the ligplot application of the Ligplus software Version 2.2 [67 (link)]. The intermolecular interactions identified in each docked complex, comprising hydrophobic interactions and hydrogen bonds, were analysed and compared to CL-316,243 and fenofibrate (experimental control standards), which are compounds previously reported to experimentally activate UCP1 and PPARα expression, respectively [53 (link),54 (link)].
Jack B.U., Ramharack P., Malherbe C., Gabuza K., Joubert E, & Pheiffer C. (2023). Cyclopia intermedia (Honeybush) Induces Uncoupling Protein 1 and Peroxisome Proliferator-Activated Receptor Alpha Expression in Obese Diabetic Female db/db Mice. International Journal of Molecular Sciences, 24(4), 3868.
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Publication 2023
Binding Sites Carbohydrates Chimera CL 316243 eriodictyol Fenofibrate flavanone Hexoses Hydrogen Bonds Hydrophobic Interactions Mice, House Molecular Docking Simulation Molecular Structure Mus Plant Roots Polyphenols Proteins UCP1 protein, human
4
Lipidome and Bioenergetics of BAT
To examine how BAT mitochondrial lipidome might be influenced by changes in thermogenic burden, we studied mice housed in cold, thermoneutrality, or with UCP1 deletion. We also used high-resolution respirometry and fluorometry to study OXPHOS and UCP1 bioenergetics in BAT mitochondria. To study the role of mitochondrial CL or PE in BAT, mice with BAT-specific deletion of CLS or PSD were generated. These mice were then subjected to whole-body and BAT mitochondrial phenotyping. Whole-body phenotyping included cold tolerance testing and measurements of whole-body oxygen consumption with or without CL-316,243. Mitochondrial phenotyping included electron microscopy, histological staining, Western blotting, and high-resolution respirometry and fluorometry. Lastly, UCP1 proton current was measured in PE-deficient mitoplasts via patch clamp with and without ATP inhibition.
Johnson J.M., Peterlin A.D., Balderas E., Sustarsic E.G., Maschek J.A., Lang M.J., Jara-Ramos A., Panic V., Morgan J.T., Villanueva C.J., Sanchez A., Rutter J., Lodhi I.J., Cox J.E., Fisher-Wellman K.H., Chaudhuri D., Gerhart-Hines Z, & Funai K. (2023). Mitochondrial phosphatidylethanolamine modulates UCP1 to promote brown adipose thermogenesis. Science Advances, 9(8), eade7864.
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Publication 2023
Bioenergetics CL 316243 Cold Temperature Deletion Mutation Electron Microscopy Fluorometry Human Body Immune Tolerance Lipidome Measure, Body Mice, Laboratory Mitochondria Mitochondrial Inheritance Oxygen Consumption Protons Psychological Inhibition Thermogenesis UCP1 protein, human
5
Assessing Cold Tolerance and BAT Activity in Mice
Cold tolerance testing was conducted on single housed mice at 4°C with access to water and light bedding. Core body temperature was measured using a temperature-sensitive transponder injected into dorsal subcutaneous adipose tissue (Bio Medic Data Systems, IPTT 300). The transponder was placed in the mice 1 week before cold tolerance testing. Transponder readings were assessed using a Reader-Programmer (Bio Medic Data Systems, DAS 8007). Whole-body VO2 was measured using the Comprehensive Lab Animal Monitoring System (Columbus Instruments). Intraperitoneal injected CL 316,243 (Sigma-Aldrich, C5976) was used to stimulate BAT activity.
Johnson J.M., Peterlin A.D., Balderas E., Sustarsic E.G., Maschek J.A., Lang M.J., Jara-Ramos A., Panic V., Morgan J.T., Villanueva C.J., Sanchez A., Rutter J., Lodhi I.J., Cox J.E., Fisher-Wellman K.H., Chaudhuri D., Gerhart-Hines Z, & Funai K. (2023). Mitochondrial phosphatidylethanolamine modulates UCP1 to promote brown adipose thermogenesis. Science Advances, 9(8), eade7864.
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Publication 2023
Animals CL 316243 Cold Temperature Human Body Immune Tolerance Light Mice, House Subcutaneous Fat

Top products related to «CL 316243»

1
Cl316 243 by Merck Group
Sourced in United States, Japan, Germany
CL316,243 is a laboratory product manufactured by Merck Group. It is a chemical compound used for research and scientific experimentation purposes. The core function of CL316,243 is to serve as a research tool, with specific details on its intended use not provided.
2
Cl316 243 by Bio-Techne
Sourced in United Kingdom, United States
CL316,243 is a synthetic compound developed by Bio-Techne. It is a selective β3-adrenergic receptor agonist, which is a class of molecules that can activate the β3-adrenergic receptor. The core function of CL316,243 is to serve as a research tool for studying the physiological roles and pharmacological properties of the β3-adrenergic receptor in various experimental models.
3
Free glycerol reagent by Merck Group
Sourced in United States, Germany, Denmark, Japan, Spain, Holy See (Vatican City State)
The Free Glycerol Reagent is a laboratory product designed to measure the concentration of free glycerol in a sample. It provides a reliable and accurate method for determining the level of free glycerol, which is a useful metric in various applications.
4
Isoproterenol by Merck Group
Sourced in United States, Germany, Japan, United Kingdom, Sao Tome and Principe, Canada, France, Denmark, Italy, Sweden
Isoproterenol is a synthetic catecholamine used as a laboratory reagent. It acts as a non-selective beta-adrenergic agonist, stimulating both beta-1 and beta-2 adrenergic receptors. Isoproterenol is commonly used in research applications to study cardiovascular and respiratory function.
5
Cl 316 243 by Cayman Chemical
Sourced in United States
CL 316,243 is a chemical compound used in laboratory research. It functions as a selective agonist for the beta-3 adrenergic receptor.
6
Bodipy 493 503 by Thermo Fisher Scientific
Sourced in United States, Germany, Italy, China, United Kingdom, Japan, France, Switzerland, Belgium
BODIPY 493/503 is a fluorescent dye that can be used to stain neutral lipids and lipid droplets. It has an excitation maximum at 493 nm and an emission maximum at 503 nm.
7
D12492 by Research Diets
Sourced in United States, China, Canada, Japan, Denmark, Montenegro, United Kingdom
The D12492 is a powdered rodent diet formulated by Research Diets. It is a highly palatable, nutrient-dense diet that provides a standardized nutritional profile for research purposes. The diet is designed to be easily administered and consumed by laboratory rodents.
8
Dexamethasone (dex) by Merck Group
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Dexamethasone is a synthetic glucocorticoid medication used in a variety of medical applications. It is primarily used as an anti-inflammatory and immunosuppressant agent.
9
Indomethacin by Merck Group
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Indomethacin is a laboratory reagent used in various research applications. It is a non-steroidal anti-inflammatory drug (NSAID) that inhibits the production of prostaglandins, which are involved in inflammation and pain. Indomethacin can be used to study the role of prostaglandins in biological processes.
10
Insulin by Merck Group
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Insulin is a lab equipment product designed to measure and analyze insulin levels. It provides accurate and reliable results for research and diagnostic purposes.

More about "CL 316243"

Discover how PubCompare.ai's cutting-edge AI-driven platform can help you optimize your research protocol for the compound CL 316243 - a beta-3 adrenergic receptor agonist commonly used in studies of lipolysis, thermogenesis, and obesity.
Easily locate relevant protocols from scientific literature, pre-prints, and patents, and leverage our intelligent comparison tools to identify the best approaches and products for your experiments.
CL 316243 is a potent and selective agonist of the beta-3 adrenergic receptor, which plays a key role in regulating energy expenditure and fat metabolism.
It has been widely used in research to study the effects of beta-3 receptor activation on processes like free glycerol release (a marker of lipolysis), BODIPY 493/503 staining (for lipid droplet imaging), and the expression of thermogenic genes like Ucp1.
By utilizing PubCompare.ai's AI-driven protocol optimization, you can enhance the reproducibility of your CL 316243-related studies and streamline your research workflows.
Experiance the power of our platform to easily find, compare, and select the most relevant protocols, whether you're investigating the effects of CL 316243 alone or in combination with other compounds like isoproterenol, dexamethasone, or indomethacin.
Get started with PubCompare.ai today and take your CL 316243 research to the next level!