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Colcemide

Colcemide is a chemical compound used in various biomedical research applications, including cell cycle analysis, chromosome staining, and mitotic spindle disruption.
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Most cited protocols related to «Colcemide»

1
Spreading and Measuring DNA Fibers
Cited 64 times
Chromosomes and nuclei were spread onto glass slides following a standard procedure. Cell cultures (5 × 105 cells/ml) were incubated in medium containing colcemid (50 ng/ml, 30 min; 37°C; Sigma Chemical Co., Ltd.), washed once in HBSS, once in PBS, swollen (0.075 M KCl, 15 min; 37°C), fixed with methanol/acetic acid (3:1), dropped (three drops; 2.5 × 106 cells/ml) onto washed slides, and then air dried.
To prepare extended DNA fibers (Parra and Windle, 1993 (link)), 2 μl cells resuspended in PBS (106 cells/ml) were spotted onto cleaned glass slides and lysed with 5 μl of 0.5% SDS in 200 mM Tris-HCl, pH 7.4, 50 mM EDTA (10 min, 20°C). Slides were tilted (15° to horizontal), allowing a stream of DNA to run slowly down the slide, air dried, and then fixed in methanol/acetic acid (3:1). For most purposes, cells containing halogenated DNA were diluted 30- and 100-fold with untreated HeLa cells, before spreading. This simplifies the spreads, allows isolated labeled DNA fibers to be found with relative ease, and makes possible the identification of replicons from a single labeled cell (see Fig. 4).
To estimate the extension of DNA fibers, spreads were prepared from HeLa cells infected with adenovirus serotype 2 and grown in medium supplemented with 100 μM BrdU 15–20 h after infection (Pombo et al., 1994 (link)). Abundant Br-labeled DNA molecules measured 13.9 ± 1.3 μm (mean ± SD; n = 50). As the viral genome is 36 kbp, the extension of these DNA fibers is 2.59 ± 0.24 kbp/μm.
Jackson D.A, & Pombo A. (1998). Replicon Clusters Are Stable Units of Chromosome Structure: Evidence That Nuclear Organization Contributes to the Efficient Activation and Propagation of S Phase in Human Cells. The Journal of Cell Biology, 140(6), 1285-1295.
Publication 1998
Acetic Acid Adenovirus Vaccine Bromodeoxyuridine Cell Culture Techniques Cell Nucleus Cells Chromosomes Colcemide DNA, A-Form Edetic Acid HeLa Cells Hemoglobin, Sickle Infection Methanol Replicon Tromethamine Viral Genome
2
Induction of Mitosis Arrest and Apoptosis
Cited 28 times
The inhibition of mitosis and the induction of apoptosis in KG1a and MV4–11 cells were induced respectively by exposure to camptothecin (Sigma-Aldrich, Saint-Quentin Fallavier, France), a cytotoxic quinoline alkaloid which inhibits the DNA enzyme topoisomerase I [10] (link), [11] (link) and by AZD8055 (AstraZeneca Cancer & Infection Research Area, Alderley Park, UK) [12] (link), a selective inhibitor of mTOR kinase, respectively. Cells were seeded at 2×105 cells/mL (5% CO2 incubator at 37°C). KG1a cells were cultured for 6h with camptothecin at a final concentration of 1 µM and MV4–11 cells were cultured for 24 h with AZD8055 at a final concentration of 10 nM and 100 nM. The stock solutions were diluted to ensure a final concentration of <0.03% for DMSO (Sigma-Aldrich). Control cultures were treated with an equivalent volume of DMSO in MEM alpha medium which did not induce apoptosis.
Quiescence was induced in KG1a cells by contact with BM MSCs [13] (link). Adherent culture-amplified MSCs were used at passage 2 (P2). KG1a cells were co-cultured on P2-MSCs for 72 h (37°C in 95% humidified air and 5% CO2) at a starting concentration of 1.5×104/cm2.
The accumulation of KG1a cells in the M phase was induced by exposure to colcemid (KaryoMax Colcemid, Life Technologies), used for arresting the dividing cell at metaphase of mitosis. Cells were cultured 30 min and 1 h with colcemid at a final concentration of 0.1 µg/mL.
Lymphocytes stimulation was induced by exposure to phytohemagglutinin (PHA) (Remel™, Oxoid™, Haarlem, The Netherlands), which is used to stimulate mitotic division of lymphocytes. Whole blood cells were cultured 72 h with PHA at a final concentration of 170 µg/mL according to the manufacturer’s recommandations.
All experiments were performed in triplicate.
Vignon C., Debeissat C., Georget M.T., Bouscary D., Gyan E., Rosset P, & Herault O. (2013). Flow Cytometric Quantification of All Phases of the Cell Cycle and Apoptosis in a Two-Color Fluorescence Plot. PLoS ONE, 8(7), e68425.
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Publication 2013
Apoptosis AZD8055 Blood Cells Camptothecin Cells Colcemide Division Phase, Cell Enzymes Infection Lymphocyte Lymphocyte Activation Malignant Neoplasms Metaphase Mitosis MTOR Inhibitors Phytohemagglutinins Plant Alkaloids Psychological Inhibition quinoline Sulfoxide, Dimethyl TOP1 protein, human
3
Chromatid Break and SCE Analysis
Cited 12 times
For PCC analysis, cells were treated with 50 ng/ml calyculin A (Calbiochem) for 30 min before harvesting. For SCE analysis, cells were grown for 48 h in BrdU before irradiation. Colcemid (together with 1 mM caffeine to overcome the G2/M arrest) was added at 8 h until 12 h post-irradiation to collect cells in mitosis. Chromatid breaks (for PCC analysis) or SCEs were scored in at least 100 chromosome spreads from at least three independent experiments per data point. Staining was according to standard protocols. Aphidicolin (Sigma) was added at 1 μg/ml immediately before IR. ATM inhibitor (KU55933) and DNA-PK inhibitor (NU7026), a kind gift from Kudos Pharmaceuticals (Cambridge, UK), were added at 20 μM 60 min prior to IR. X-ray irradiation was performed at 90- or 120-kV γ-irradiation using a 137Cs source. Dosimetry was performed with ion chambers and considered the increase in dose for cells grown on glass coverslips relative to plastic surfaces (Kegel et al, 2007 (link)).
Beucher A., Birraux J., Tchouandong L., Barton O., Shibata A., Conrad S., Goodarzi A.A., Krempler A., Jeggo P.A, & Löbrich M. (2009). ATM and Artemis promote homologous recombination of radiation-induced DNA double-strand breaks in G2. The EMBO Journal, 28(21), 3413-3427.
Publication 2009
Aphidicolin Bromodeoxyuridine Caffeine calyculin A Cells Chromatids Chromosomes Colcemide KU 55933 Mitosis NU 7026 Pharmaceutical Preparations Protein Kinase, DNA-Dependent Radiometry Radiotherapy X-Rays, Diagnostic
4
Metaphase Spread Preparation from MEFs and Splenocytes
Cited 11 times
To prepare metaphase spreads from MEFs, 1–2 × 106 cells (at passage 5) were treated with 0.05 μg/ml colcemid (GIBCO BRL) for 4–5 h at 37°C. To prepare metaphase spreads from splenocytes (Rudolph et al., 1999 (link)), spleens were freshly collected and minced between two microscope slides. Released cells were suspended in 5 ml PBS, centrifuged at 1,000 rpm for 5 min, resuspended in 4 ml of RPMI containing 10% FBS, IL-2 (10 U/ml), PHA (5 μg/ml), conA (5 μg/ml), and colcemid (0.05 μg/ml), and cultured for 6–7 h at 37°C. After colcemid treatment, MEF or splenocyte cells were harvested, suspended in 5 ml 0.075 M KCl, and incubated at RT for 30 min. Cells were fixed in Carnoy's solution (75% methanol, 25% acetic acid), washed, and finally resuspended in ∼0.5 ml fixative. 25-μl aliquots were dropped onto prewetted microscope slides, stained for 10 min in 5% Giemsa solution, and analyzed on an Olympus AX70 microscope using a 100× objective.
Babu J.R., Jeganathan K.B., Baker D.J., Wu X., Kang-Decker N, & van Deursen J.M. (2003). Rae1 is an essential mitotic checkpoint regulator that cooperates with Bub3 to prevent chromosome missegregation. The Journal of Cell Biology, 160(3), 341-353.
Publication 2003
Acetic Acid Carnoy's solution Cells Colcemide Concanavalin A Fixatives Metaphase Methanol Microscopy Stain, Giemsa
5
Metaphase Chromosome Analysis Protocol
Cited 11 times
Plates containing primary MEFs or established cell lines were treated with colcemid (0.1 μg/ml) for 4–5 h and subsequently trypsinized and spun for 8 min at 120 g. After hypotonic swelling in sodium citrate (0.03 M) for 25 min at 37°C, cells were fixed in methanol/acetic acid (3:1). After 2–3 additional changes of fixative, cell suspensions were dropped on wet, clean slides and dried overnight. FISH with Cy-3 labeled (CCCTAA)3 peptide-nucleic acid, and subsequent quantitative analysis of digital images were performed as described (Zijlmans et al., 1997 (link)). The slide coordinates of the metaphase images captured were noted to relocate the same metaphase after FISH with minor satellite DNA (see below) and mouse chromosome probes (Oncor).
Hande M.P., Samper E., Lansdorp P, & Blasco M.A. (1999). Telomere Length Dynamics and Chromosomal Instability in Cells Derived from Telomerase Null Mice. The Journal of Cell Biology, 144(4), 589-601.
Publication 1999
Acetic Acid Cell Lines Cells Chromosomal Probes Colcemide DNA, Satellite Fishes Fixatives Metaphase Methanol Mus Peptide Nucleic Acids Sodium Citrate

Most recents protocols related to «Colcemide»

1
FISH Analysis of ESC Chromosome Loci
FISH analyses were carried on both metaphase arrested and cycling interphase nuclei of ESCs. The probes were purchased from Empire Genomics, USA (Catalog # MYBPC3-20GR and MYH7-20-OR). FISH probes specific for MYBPC3 (11p11.2 locus, ~188Kb) were labeled using Green-dUTP, and for MYH7 (14q11.2 locus, ~177 Kb) were labeled using Orange-dUTP. Briefly, ESCs were treated with KaryoMAX Colcemide (Life Technologies) at a final concentration of 200 ng/mL for 1.5 h at 37 °C. Treated cells were then detached by 0.25% trypsin/EDTA and incubated in hypotonic 0.075 M KCL for 20 min. Cells were next fixed with methanol: acetic acid (3:1 v/v) and dropped onto a slide and dried on a hot plate at 60 °C. The samples were dehydrated using ethanol (70, 85, and 100%) for 1 min in each and dried in air. Slides were applied with the probe mixture, covered with an 18 mm2 coverslip, and incubated in a humidified Thermobrite® system (Leica) set at 73 °C for 2 min, and then 37 °C for 16 h. The incubated slides were rinsed with washing solution 1 (0.3% Igepal/0.4 × SSC) and washing solution 2 (0.1% Igepal/2 × SSC). Slides were mounted in ProLong™ Gold Antifade Mountant with DAPI (Life Technologies) and observed using a fluorescence microscopy equipped with a cooled CCD camera. Images were captured and analyzed by ISIS analysis software (MetaSystem GmbH).
Liang D., Mikhalchenko A., Ma H., Marti Gutierrez N., Chen T., Lee Y., Park S.W., Tippner-Hedges R., Koski A., Darby H., Li Y., Van Dyken C., Zhao H., Wu K., Zhang J., Hou Z., So S., Han J., Park J., Kim C.J., Zong K., Gong J., Yuan Y., Gu Y., Shen Y., Olson S.B., Yang H., Battaglia D., O’Leary T., Krieg S.A., Lee D.M., Wu D.H., Duell P.B., Kaul S., Kim J.S., Heitner S.B., Kang E., Chen Z.J., Amato P, & Mitalipov S. (2023). Limitations of gene editing assessments in human preimplantation embryos. Nature Communications, 14, 1219.
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Publication 2023
Acetic Acid Cell Nucleus Cells Colcemide DAPI deoxyuridine triphosphate Edetic Acid Enhanced S-Cone Syndrome Ethanol Fishes Gold Interphase Metaphase Methanol Microscopy, Fluorescence Trypsin
2
iPSC Karyotyping and Pluripotency Evaluation
IPSCs were karyotyped in metaphase by the Shivanand R. Patil Cytogenetics and Molecular Laboratory at the University of Iowa using Leica Microsystems metaphase scanning platform and CytoVision version 7.7 software. Cells were grown in vitro and arrested at metaphase with colcemid. Chromosomes were stained by the G-banding method, counted and structurally evaluated for the presence or absence of detectable rearrangements. At least 20 cells were analyzed for each iPSC line. For TaqMan hPSC ScoreCard™ analysis, total RNA was isolated using the NucleoSpin RNA purification kit (Takara Bio, San Jose, CA). cDNA was generated from 1 µg of RNA using VILO cDNA synthesis kit (Thermo Fisher Scientific). cDNA was added to a TaqMan hPSC scorecard plate (Thermo Fisher Scientific) and amplified using a QuantStudio 6 Flex real-time PCR system. Results were analyzed using Thermo Fisher’s cloud-based analysis suite.
Bohrer L.R., Stone N.E., Mullin N.K., Voigt A.P., Anfinson K.R., Fick J.L., Luangphakdy V., Hittle B., Powell K., Muschler G.F., Mullins R.F., Stone E.M, & Tucker B.A. (2023). Automating iPSC generation to enable autologous photoreceptor cell replacement therapy. Journal of Translational Medicine, 21, 161.
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Publication 2023
Anabolism Cells Chromosomes Colcemide DNA, Complementary Gene Rearrangement Induced Pluripotent Stem Cells Metaphase Staining
3
Cell Proliferation and Cell Cycle Analysis
Cells proliferation was assayed using the Muse Count & Viability Assay (Luminex), according to manufacturer’s instructions. Cells (1 × 105) were seeded in a multiwell-24 plate in 1 mL of complete medium. GEs were added and cells collected 24 h p.t., washed with PBS and resuspended in 50 µL of PBS and 450 µL of Muse Count & Viability Reagent. After an incubation of 5 min at RT, cells were analyzed at the cytofluorometer.
Cell cycle analysis was performed as described [62 (link)]. Cells (2.5 × 105) were seeded in a multiwell-6 plate in 2.5 mL of complete medium. Colcemid (10 µg/mL) was added to synchronize cells in metaphase. After 24 h, colcemid was removed by substituting the culture medium and GEs were administered for additional 24 h. Cells were then collected, centrifuged at 300× g for 5 min and fixated overnight with 200 µL of 70% ethanol. Subsequently, cells were centrifuged and resuspended in propidium iodide (50 µg/mL) and RNAse A (0.1 mg/mL). The samples were analyzed after an overnight incubation at 4 °C by BD FACSLyrics (BD Bioscience, Franklin Lakes, NJ, USA).
Rabbit polyclonal Cyclin E2 (clone H-140) antibody form Santa Cruz Biotechnologies (Dallas, TX, USA) and a mouse monoclonal Cyclin A (clone CY-A1) antibody (Sigma) were used to analyze cell cycles as follows: 2 μg of each antibody were fluorescently conjugated using DyLight555 labelling kit (Biorad, Hercules, CA, USA) as described [63 (link)]. SUP-T1 cells (about 105 for each sample), treated for 24 h with the following micromolar amounts of GEs (i.e., A4 = 70; A5 = 60; B4 = 3.4; B5 = 0.02; C5 = 13.5, according to BIOPEP database) were then fixed and permeabilized using ice cold methanol for 15 min, washed twice with ice cold PBS and then blocked with PBS containing 1% Fetal Calf Serum (FCS) for 30 min at room temperature. After PBS washing, cells were incubated with 2 μL of each fluorescently labelled antibody in a PBS + FCS 1% volume of 50 μL for 2 h at room temperature. Finally, cells were washed twice with D-PBS and analyzed for fluorescence intensity using Guava Muse Cell Analyser (Luminex, Austin, TX, USA), analyzing 2000 events in two independent replicas.
Manai F., Zanoletti L., Morra G., Mansoor S., Carriero F., Bozzola E., Muscianisi S, & Comincini S. (2023). Gluten Exorphins Promote Cell Proliferation through the Activation of Mitogenic and Pro-Survival Pathways. International Journal of Molecular Sciences, 24(4), 3912.
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Publication 2023
austin Biological Assay CCNE2 protein, human Cell Cycle Cell Proliferation Cells Clone Cells Colcemide Cold Temperature Culture Media Cyclin A Endoribonucleases Ethanol Fetal Bovine Serum Fluorescence Immunoglobulins Metaphase Methanol Mus Muse Propidium Iodide Psidium guajava Rabbits
4
Chromosome Karyotyping Protocol
The metaphases were arrested by incubation with Colcemid (#15210-040, KaryoMax Colcemid Solution (Invitrogen, Carlsbad, CA, USA) (10 ug/mL) 2 h prior to harvest. Cells were collected and treated with hypotonic solution (#685804, KCL 0.075M, Macron Chemical, Radnor, PA, USA) for 15 min at 37 °C and fixed with 3:1 ratio of methanol: acetic acid. Slides were prepared and aged overnight for use in G-band, SKY analysis. Chromosomes were stained with a trypsin- Giemsa staining technique [22 (link)]. Analyses were performed under an Axioplan ImagerZ2 (Zeiss, Dublin, CA, USA) microscope coupled with a CCD camera; images were captured with ASI software, (Applied Spectral Imaging Inc., Vista, CA, USA). The karyotype was determined by comparison to the standard ideogram of banding patterns for human chromosomes by ISCN 2009.
Cheng R.Y., Burkett S., Ambs S., Moody T., Wink D.A, & Ridnour L.A. (2023). Chronic Exposure to Nitric Oxide Induces P53 Mutations and Malignant-like Features in Human Breast Epithelial Cells. Biomolecules, 13(2), 311.
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Publication 2023
Acetic Acid Cells Chromosomes Chromosomes, Human Colcemide Hypotonic Solutions Karyotyping Metaphase Methanol Microscopy Trypsin
5
Cytogenetic Analysis of Ionizing Radiation Damage
For metaphase analysis, after irradiation Cal 51 cells were allowed to grow in a complete medium at 37 ℃ and 5% CO2. Cells were fixed at 16 h after exposure, proceeded by a 1 h colcemid treatment (50 ng/ml) for metaphase accumulation, and stained with 3% Giemsa.
After exposure to proton beams and 60Co γ-rays the blood samples (resting PBL at G0 cell cycle stage) were diluted in 4.5 ml of RPMI medium supplemented by 20% fetal calf serum, 2 mM L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin and 1.5% phytohaemagglutinin (PHA), incubated at 37° C and 5% CO2, fixed at 48 h after PHA stimulation, proceeded by a 3 h colcemid treatment (200 ng/ml) for metaphase accumulation, and stained with 3% Giemsa. Typically, 100–300 metaphases were analyzed for every data point. Chromosomal aberrations were classified according to Savage (1976). All aberrations of the chromosome and chromatid types visible without karyotyping were recorded. The chromosome-type aberrations comprise paired fragments, dicentrics, centric and acentric rings (the latter also includes double minutes) and translocations visible without karyotyping. The chromatid-type aberrations include the chromatid-type breaks and chromatid-type exchanges. The gaps were not scored as aberrations.
For the PCC analysis, PBL were isolated from the blood by gradient centrifugation using BD Vacutainer® CPT™ (Becton, Dickinson and Co., USA) and cultured in the same RPMI medium 48 h prior to irradiation. Exponentially growing lymphocytes and Cal 51 cells were allowed to repair for various times after irradiation (0–12 h) and then were forced to condense chromatin prematurely by addition of 50 nM calyculin A (Sigma) immediately after irradiation and left for 1 h in 37° C. Then, the cells were treated with 0.075 M KCl for 10–15 min at 37 ℃ and fixed with methanol:glacial acetic acid (3:1). Cells were dropped onto a clean wet slide, airdried and stained with 3% Giemsa. Typically, 100–200 G2-phase cells were analyzed for every data point. The scoring and recording criteria followed those given in IAEA Manual (2011 ). The damage was classified as chromatid breaks, isochromatid breaks (excess figures) and chromatid exchanges (Kowalska et al. 2020 (link)). The yield of isochromatid breaks was measured from the excess number of chromosomes (> 46 figures) observed (IAEA 2011 ). In G2-phase of the cell cycle, the isochromatid break occurs when two breaks are formed on the opposite sister chromatids in a close proximity. Since one isochromatid break results from the breakage of both chromatid threads, one isochromatid break was scored as two chromatid breaks. Exchanges were also scored as two breaks. For further details, see (Kowalska et al. 2020 (link)).
Kowalska A., Nasonova E., Kutsalo P, & Czerski K. (2023). Chromosomal radiosensitivity of human breast carcinoma cells and blood lymphocytes following photon and proton exposures. Radiation and Environmental Biophysics, 62(1), 151-160.
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Publication 2023
Acetic Acid BLOOD calyculin A Cell Cycle Cells Centrifugation Chromatids Chromatin Chromosome Aberrations Chromosomes Colcemide Culture Media Fetal Bovine Serum G2 Phase Glutamine Lymphocyte Metaphase Methanol Penicillins Phytohemagglutinins Protons Radiation Radiotherapy Resting Phase, Cell Cycle Streptomycin Translocation, Chromosomal

Top products related to «Colcemide»

1
Colcemid by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany
Colcemid is a chemical compound primarily used in cell biology research. It functions as a mitotic inhibitor, arresting cells in metaphase of the cell division cycle. Colcemid disrupts microtubule formation, thereby preventing the proper segregation of chromosomes during mitosis.
2
Colcemid by Merck Group
Sourced in United States, Germany, United Kingdom
Colcemid is a chemical compound used in laboratory settings for applications involving cell biology and cytogenetics. It functions by disrupting the formation of the mitotic spindle during cell division, leading to the arrest of cells in metaphase. This property makes Colcemid a valuable tool for researchers studying cellular processes and chromosome structure.
3
Karyomax colcemid by Thermo Fisher Scientific
Sourced in United States
KaryoMAX colcemid is a laboratory reagent used in cytogenetic analysis. It functions to arrest cells in metaphase, allowing for the visualization and analysis of chromosomes. The product is intended for research use only and not for use in diagnostic procedures.
4
Karyomax colcemid solution by Thermo Fisher Scientific
Sourced in United States, Ireland, Switzerland
KaryoMAX Colcemid Solution is a laboratory reagent used for arresting mitosis in cell cultures. It acts by disrupting the formation of the mitotic spindle, thereby preventing cell division. The solution is designed for use in cytogenetic applications, such as karyotyping and chromosome analysis.
5
Karyomax by Thermo Fisher Scientific
Sourced in United States
The KaryoMAX is a laboratory instrument designed for chromosome analysis. It provides high-resolution imaging and automated analysis of metaphase chromosome spreads for applications such as karyotyping and cytogenetic research.
6
Colcemid by Roche
Sourced in Switzerland, United States, Germany
Colcemid is a chemical compound used in laboratory research and cell biology applications. It acts as a mitotic inhibitor, preventing the formation of the mitotic spindle during cell division. Colcemid is commonly used to arrest cells in metaphase, facilitating the analysis of chromosomes and cellular processes.
7
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
8
Giemsa by Merck Group
Sourced in United States, Germany, Japan, Italy, United Kingdom, Spain, Belgium, Canada, China, France, Australia, Singapore, Brazil
Giemsa is a type of stain used in microscopy for the differential staining of cells, tissues, and other biological samples. It is a widely used stain in various fields, including hematology, parasitology, and cytology. Giemsa stain primarily helps to visualize and differentiate cellular components, such as nuclei, cytoplasm, and specific organelles, based on their varying affinity for the stain.
9
Colcemid solution by Thermo Fisher Scientific
Sourced in United States, Germany
Colcemid solution is a laboratory reagent used to arrest cell division at metaphase. It functions by inhibiting the polymerization of microtubules, which are essential for cell division. This solution is commonly used in cytogenetic analyses and cell biology research.
10
Trypsin edta by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Canada, Switzerland, France, China, Australia, Japan, Italy, Spain, New Zealand, Sweden, Singapore, Portugal, Thailand, Belgium, Denmark, Taiwan, Province of China, Ireland, Brazil, Netherlands, Austria, Czechia, India, Morocco, Israel, Poland, Macao
Trypsin-EDTA is a solution used in cell culture applications to dissociate adherent cells from their growth surface. It contains the proteolytic enzyme trypsin and the chelating agent EDTA, which work together to break down the cellular adhesions and allow the cells to be harvested and passaged.

More about "Colcemide"

Colcemide, also known as Colcemid, is a chemical compound widely used in various biomedical research applications.
It is a potent microtubule-depolymerizing agent that disrupts the mitotic spindle, making it a valuable tool for cell cycle analysis, chromosome staining, and the study of mitotic processes.
Colcemide is closely related to Colcemid, another commonly used compound in research.
Both Colcemide and Colcemid are derived from the plant alkaloid colchicine and share similar mechanisms of action.
Colcemide, along with its commercial formulations like KaryoMAX Colcemid Solution, is often used in combination with other reagents like Giemsa stain and Trypsin-EDTA to facilitate karyotyping, chromosome analysis, and cell culture manipulation.
The use of Colcemide, Colcemid, and related products like FBS (Fetal Bovine Serum) is crucial in various research fields, including cell biology, genetics, and drug discovery.
By disrupting the mitotic spindle, Colcemide arrests cells in metaphase, allowing researchers to study chromosome structure, segregation, and other mitotic processes in detail.
PubCompare.ai, a cutting-edge platform, can enhance your Colcemide-related research by helping you locate relevant protocols from literature, preprints, and patents.
The AI-driven comparison tools offered by PubCompare.ai can assist in identifying the best protocols and products, ultimately improving the reproducibility and accuracy of your Colcemide research.