To be included, men and women had to be at least 18 years of age, have a prior diagnosis of IBS made by a health care provider, and had to report current IBS symptoms (Rome-II criteria). Participants were excluded if they had a history of co-existing GI pathology (e.g., inflammatory bowel disease, celiac disease) or surgery (e.g., bowel resection), renal, or reproductive pathology (e.g., endometriosis, prostate cancer). Participants with certain other comorbidities or medication use were also excluded, based on the guiding principle of whether the disorder or medications could confound the measurement of the symptoms of IBS or compromise the subject’s ability to complete the study. Subjects were excluded for conditions such as severe fibromyalgia, type 1 or 2 diabetes mellitus, infectious diseases (e.g., hepatitis B or C, HIV), symptoms of dementia, untreated sleep apnea/hypopnea, severe cardiovascular disease, severe depression, and current substance abuse. Examples of medications that lead to exclusion included the regular use of antibiotics, anticholinergics, cholestyramine, narcotics, colchicine, docusate, enema preparations, iron supplements, or laxatives. Human subjects institutional review approval was obtained prior to enrolling participants (May 2002) and renewed yearly thereafter. This study was registered with clinicaltrials.gov through the U.S. National Institutes of Health.
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Colchicine
Colchicine
Colchicine is a naturally occuring alkaloid compound derived from the autumn crocus (Colchium autumnale) plant.
It has a long history of medicinal use, primarily as an anti-inflammatory and gout treatment.
Colchicine works by disrupting microtubule formation, inhibiting cell division and migration.
This mechanism makes it useful for treating conditions associated with inflammation and crystal deposition, such as gout, familial Mediterranean fever, and pericarditis.
Reserchers leveraging PubCompare.ai can optimize their Colchicine research protocols by locating the best published methods and products, enhancing reproducibility and accuracy.
It has a long history of medicinal use, primarily as an anti-inflammatory and gout treatment.
Colchicine works by disrupting microtubule formation, inhibiting cell division and migration.
This mechanism makes it useful for treating conditions associated with inflammation and crystal deposition, such as gout, familial Mediterranean fever, and pericarditis.
Reserchers leveraging PubCompare.ai can optimize their Colchicine research protocols by locating the best published methods and products, enhancing reproducibility and accuracy.
Most cited protocols related to «Colchicine»
Antibiotics
Anticholinergic Agents
Apnea
Cardiovascular Diseases
Celiac Disease
Colchicine
Communicable Diseases
Dementia
Diabetes Mellitus
Diagnosis
Dietary Supplements
Docusate
Endometriosis
Enema
Fibromyalgia
Health Personnel
Hepatitis B
Homo sapiens
Inflammatory Bowel Diseases
Intestines
Iron
Kidney
Laxatives
Narcotics
Operative Surgical Procedures
Pharmaceutical Preparations
Prostate Cancer
Reproduction
Resin, Cholestyramine
Sleep Apnea Syndromes
Substance Abuse
Woman
We analyzed ninety-six specimens of H. obliquidens (46 males, 33 females and 17 of undetermined sex) obtained from the aquarium trade in Botucatu, SP, Brazil (Table 1 ). The animal experiments were performed with the approval of the appropriate ethics committee of UNESP - São Paulo State University. Metaphase chromosomes were obtained from cells of the anterior kidney following in vivo treatment with colchicine at 0.025% (1 ml/100 g of body weight) according to the air-drying method [59 ]. Heterochromatin was identified by C-banding [60 (link)], and the nucleolus organizer regions (NORs) were visualized by silver nitrate staining [61 (link)]. Chromosomes were classified as meta/submetacentric (m/sm) and subtelo/acrocentric (st/a), and were organized by decreasing order of size in the karyotype. Meiotic cells from testes were obtained as described by Kligerman and Bloom [62 ].
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Body Weight
Cells
Chromosomes
Colchicine
Ethics Committees
Females
Germ Cells
Head Kidney
Heterochromatin
Karyotype
Males
Metaphase
Nucleolus Organizer Region
Testis
Pdyn-ires Cre, Avp-ires-Cre, Crh-ires-Cre, and Pacap-ires-Cre mice were crossed to R26-loxSTOPlox-L10-GFP mice, sacrificed and sectioned at 30 μm. Brain sections were washed in 0.1 M phosphate-buffered saline with Tween 20, pH 7.4 (PBST, 2 changes) and then incubated in the primary antiserum (anti-GFP, Abcam (1:1000), rabbit anti-hrGFP. After several washes in PBS, sections were incubated in Alexa fluorophore secondary antibody (Molecular Probes, 1:200) for 2 hours at room temperature. After several washes in PBS, sections were mounted onto gelatin-coated slides and fluorescent images were captured with Olympus VS120 Slide Scanner microscope.
Trh-ires-Cre mice were stereotaxically injected with Cre-dependent AAV8-DIO-mCherry,8 (link) due to transient, early embryonic expression and subsequent deletion of floxed alleles by this mouse line, sacrificed and sectioned at 30 μm. Native fluorescence was used and fluorescent images were captured with Olympus VS120 Slide Scanner microscope. All digital images were processed in the same way between experimental conditions to avoid artificial manipulation between different data sets.
In situ mRNA hybridization experiments were performed for Pdyn-ires-Cre, Trh-ires-Cre and Pacap-ires-Cre mice (details below). Antibody staining experiments were performed for Avp-ires-Cre and Crh-ires-Cre mice. Briefly, Avp-ires-Cre; R26-loxSTOPlox-L10-GFP mice were co-localized with anti-AVP, Sigma (1:500), rabbit anti-AVP and anti-GFP, Abcam (1:1000), chicken anti-GFP. 48 hrs ICV colchicine treatment (Sigma, 10 ug), Crh-ires-Cre; R26-loxSTOPlox-L10-GFP mice were co-localized with anti-CRF, Peninsula Laboratories (1:2500), rabbit anti-CRF and anti-GFP, Abcam (1:1000), chicken anti-GFP.
All images were subsequently compared to in situ mRNA expression profiles generated by ©2013 Allen Institute for Brain Science; Allen Mouse Brain Atlas [Internet].
Trh-ires-Cre mice were stereotaxically injected with Cre-dependent AAV8-DIO-mCherry,8 (link) due to transient, early embryonic expression and subsequent deletion of floxed alleles by this mouse line, sacrificed and sectioned at 30 μm. Native fluorescence was used and fluorescent images were captured with Olympus VS120 Slide Scanner microscope. All digital images were processed in the same way between experimental conditions to avoid artificial manipulation between different data sets.
In situ mRNA hybridization experiments were performed for Pdyn-ires-Cre, Trh-ires-Cre and Pacap-ires-Cre mice (details below). Antibody staining experiments were performed for Avp-ires-Cre and Crh-ires-Cre mice. Briefly, Avp-ires-Cre; R26-loxSTOPlox-L10-GFP mice were co-localized with anti-AVP, Sigma (1:500), rabbit anti-AVP and anti-GFP, Abcam (1:1000), chicken anti-GFP. 48 hrs ICV colchicine treatment (Sigma, 10 ug), Crh-ires-Cre; R26-loxSTOPlox-L10-GFP mice were co-localized with anti-CRF, Peninsula Laboratories (1:2500), rabbit anti-CRF and anti-GFP, Abcam (1:1000), chicken anti-GFP.
All images were subsequently compared to in situ mRNA expression profiles generated by ©2013 Allen Institute for Brain Science; Allen Mouse Brain Atlas [Internet].
Alleles
Brain
Chickens
Colchicine
Deletion Mutation
Embryo
Fluorescence
Gelatins
Immune Sera
Immunoglobulins
In Situ Hybridization
Internal Ribosome Entry Sites
Mice, Laboratory
Microscopy
Molecular Probes
Phosphates
Pituitary Adenylate Cyclase-Activating Polypeptide
Rabbits
RNA, Messenger
Saline Solution
Training Programs
Transients
Tween 20
Detailed information about Ae. tauschii accessions used in this project, their photos, taxonomy of Ae. tauschii, and its relationship to wheat can be found on our website (http://aegilops.wheat.ucdavis.edu/ATGSP/ ). In brief, Ae. tauschii accession AL8/78 was provided by V. Jaaska, who collected it near the Hrazdan River, Jerevan, Armenia. The accession is classified as Ae. tauschii ssp. strangulata. We selected this accession for the construction of BAC libraries and the Ae. tauschii physical map7 (link),32 ,33 (link) because of its genetic proximity to the wheat D genome34 . The accession was also used for the construction of a genome-wide optical BioNano genome (BNG) map. We maintain this accession, which can be requested from the corresponding author (J.D.).
A more recent study of genetic relationships between Ae. tauschii and wheat uncovered a group of accessions in Caspian Iran that appeared even more closely related to the wheat D genome than AL8/7835 (link). They belong to Ae. tauschii ssp. tauschii var. meyeri. We selected from this population accession CIae2335 (link) for the construction of the second Ae. tauschii BNG map. CIae23 was made available by the US National Plant Germplasm System.
The third accession relevant to this project is wheat (T. aestivum) cv. Chinese Spring. We used accession DV418, which is derived from a colchicine-doubled haploid maintained by J.D. at UC Davis, and used it for the construction of a genome-wide BNG map of wheat.
A more recent study of genetic relationships between Ae. tauschii and wheat uncovered a group of accessions in Caspian Iran that appeared even more closely related to the wheat D genome than AL8/7835 (link). They belong to Ae. tauschii ssp. tauschii var. meyeri. We selected from this population accession CIae2335 (link) for the construction of the second Ae. tauschii BNG map. CIae23 was made available by the US National Plant Germplasm System.
The third accession relevant to this project is wheat (T. aestivum) cv. Chinese Spring. We used accession DV418, which is derived from a colchicine-doubled haploid maintained by J.D. at UC Davis, and used it for the construction of a genome-wide BNG map of wheat.
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Aegilops
Chinese
Colchicine
Genome
Physical Examination
Plants
Reproduction
Rivers
Triticum aestivum
Vision
Third instar larval brains were fixed and stained as described previously (Martinez-Campos et al., 2004 (link)). For drug treatment, dissected brains were incubated for 2 h at 25°C in PBS containing 1 μg/ml colchicine before fixation. The following primary antibodies were used: rabbit anti–D-PLP at 1–2 μg/ml (Martinez-Campos et al., 2004 (link)); mouse anti–α-tubulin at 1:1,000 (DM1α; Sigma-Aldrich), mouse anti–γ-tubulin at 1:1,000 (GTU88; Sigma-Aldrich), and mouse anti–phospho-Histone H3 at 1:2,000 (Abcam). All secondary antibodies coupled to the appropriate fluorophore (Alexa 488 or 568; Invitrogen) were used at 1:1,000 in PBT. Fixed preparations were examined on a widefield upright microscope (Axioskop II; Carl Zeiss MicroImaging, Inc.), equipped with a camera (CoolSnap HQ; Photometrics) and MetaMorph software (Molecular Devices), using a 100×/1.3 NA objective. Images of fixed brain cells are all maximum intensity projections of optical sections acquired at 0.1– 0.2-μm intervals.
alpha-Tubulin
anti-d antibody
Antibodies
Brain
Cells
Colchicine
Histone H3
Larva
Medical Devices
Microscopy
Mus
Pharmaceutical Preparations
Rabbits
Tubulin
Vision
Most recents protocols related to «Colchicine»
Example 6
A suitable in vitro model for studying the anti-tumor effects of drugs is utilized for this test. Such a test may include commonly used tumor cell lines with or without combination with cultivated PBMCs. Colchicine inhibits or reduces growth of tumor cell lines upon incubation with therapeutically effective amount of colchicine and/or cultivation with PBMCs pretreated with a therapeutically effective amount of colchicine.
Many modifications and other embodiments of the embodiments set forth herein will come to mind to one skilled in the art to which these embodiments pertain having the benefit of the teachings presented in the foregoing descriptions and the associated drawings. Therefore, it is to be understood that the presently disclosed features are not to be limited to the specific embodiments disclosed and that modifications and other embodiments are intended to be included within the scope of the appended claims and list of embodiments disclosed herein. Although specific terms are employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation.
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Antineoplastic Agents
Cardiac Arrest
Cell Line, Tumor
Colchicine
Generic Drugs
Neoplasms
Teaching
Example 4
To assess the therapeutic effects of a sustained release formulation in subjects with or in subjects with an increased risk of cancer, a prospective randomized observer blinded end-point trial was conducted to determine whether adding 0.5 mg/day of colchicine to standard anti-cancer therapies inhibits, delays, or reduces tumor growth or metastases in a cancer subject.
A sufficient amount of subjects with cancer or in risk of cancer are randomized to long-term low dose sustained release colchicine or to standard therapy alone. Cancer incidence, morbidity and mortality are analyzed in regular intervals. Subjects in the colchicine arm have a significantly lower cancer incidence and mortality than subjects not treated with colchicine.
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Cardiac Arrest
Colchicine
Malignant Neoplasms
Neoplasm Metastasis
Neoplasms
Sustained-Release Preparations
Therapeutic Effect
Example 5
A sufficient amount of specimen of a suitable animal species are included in this study utilizing commonly used tumor models. A therapeutically effective amount of colchicine is administered to the test animals. Placebo is administered to a control group. Tumor growth is induced in all animals. In animals treated with colchicine, tumor growth is prevented and/or reduced as compared to the control group.
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Animals
Colchicine
Neoplasms
Placebos
We used three cytogenetic entities: diploids and established tetraploids collected in natural populations along a latitudinal gradient that spans from Aveiro (Portugal) in the south, to the north of Galicia (Spain; Table 1 Table 1
Ten days before the start of the water stress experiment in the greenhouse, seeds from the nine populations (three diploid, five tetraploid and one neotetraploid) were placed in individual Petri dishes with filter paper moistened with distilled water and stored at 4°C for 5 days. This cold treatment enabled the synchronization of seed germination (Castro et al., 2018 (link)) and ensured that all the seedlings had similar sizes at the time of transplant to pots. After that period, Petri dishes were transferred to a climatic chamber at 24°C with a 16h:8h (light:dark) photoperiod.
Ten days before the start of the water stress experiment in the greenhouse, seeds from the nine populations (three diploid, five tetraploid and one neotetraploid) were placed in individual Petri dishes with filter paper moistened with distilled water and stored at 4°C for 5 days. This cold treatment enabled the synchronization of seed germination (Castro et al., 2018 (link)) and ensured that all the seedlings had similar sizes at the time of transplant to pots. After that period, Petri dishes were transferred to a climatic chamber at 24°C with a 16h:8h (light:dark) photoperiod.
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Aftercare
Climate
Colchicine
Common Cold
Diploidy
Germination
Grafts
Husband
Hyperostosis, Diffuse Idiopathic Skeletal
Light
Marijuana Abuse
Plants
Population Group
Seedlings
Strains
Tetraploidy
Water Stress
Information on ongoing treatment was collected, including non-steroidal anti-inflammatory drugs (NSAIDs), glucocorticoids (GCs), analgesics, joint infiltration agents, disease-modifying anti-rheumatic drugs (DMARDs), biologic agents, colchicine, contraception and homeopathy.
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Analgesics
Anti-Inflammatory Agents, Non-Steroidal
Antirheumatic Drugs, Disease-Modifying
Biological Factors
Colchicine
Contraceptive Methods
Glucocorticoids
Homeopathy
Joints
Top products related to «Colchicine»
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Colchicine is a chemical compound used in various laboratory and research applications. It is a naturally occurring alkaloid derived from the autumn crocus plant. Colchicine is primarily used as a research tool to study cell division and microtubule dynamics.
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Nocodazole is a synthetic compound that acts as a microtubule-destabilizing agent. It functions by binding to and disrupting the polymerization of microtubules, which are essential components of the cytoskeleton in eukaryotic cells. This property makes Nocodazole a valuable tool in cell biology research for studying cell division, cell motility, and other cellular processes that rely on the dynamics of the microtubule network.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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Paclitaxel is a pharmaceutical compound used in the production of various cancer treatment medications. It functions as a microtubule-stabilizing agent, which plays a crucial role in the development and regulation of cells. Paclitaxel is a key ingredient in the manufacture of certain anti-cancer drugs.
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Giemsa is a type of stain used in microscopy for the differential staining of cells, tissues, and other biological samples. It is a widely used stain in various fields, including hematology, parasitology, and cytology. Giemsa stain primarily helps to visualize and differentiate cellular components, such as nuclei, cytoplasm, and specific organelles, based on their varying affinity for the stain.
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Cytochalasin D is a laboratory reagent that inhibits actin polymerization. It is commonly used in cell biology research to disrupt the cytoskeleton and study its role in cellular processes.
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
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Vinblastine is a pharmaceutical compound used in the production of laboratory equipment. It is a naturally-derived alkaloid with a specific chemical structure. Vinblastine is employed in various research and analytical applications, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.
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Colchicine is a chemical compound commonly used in research laboratories. It functions as a microtubule-disrupting agent, inhibiting the polymerization of microtubules. This property makes it a valuable tool for studying cellular processes and structures that rely on microtubule dynamics.
More about "Colchicine"
Colchicine, a naturally occurring alkaloid compound derived from the autumn crocus (Colchium autumnale) plant, has a long history of medicinal use, primarily as an anti-inflammatory and gout treatment.
This phytochemical works by disrupting microtubule formation, inhibiting cell division and migration.
This unique mechanism makes colchicine useful for treating conditions associated with inflammation and crystal deposition, such as gout, familial Mediterranean fever, and pericarditis.
Researchers can optimize their colchicine research protocols by utilizing the AI-driven platform PubCompare.ai.
This tool helps identify the best published methods and products, enhancing reproducibility and accuracy.
PubCompare.ai allows researchers to locate protocols from literature, pre-prints, and patents, and leverages AI-driven comparisons to pinpoint the optimal approaches for their colchicine studies.
In addition to colchicine, other microtubule-targeting agents like nocodazole, paclitaxel, vinblastine, and cytochalasin D play important roles in cell biology research.
These compounds, along with cell culture media like DMEM and serum supplements like FBS, are commonly used in conjunction with colchicine to investigate cellular processes like mitosis, migration, and cytoskeletal dynamics.
Staining techniques like Giemsa can also be employed to visualize the effects of colchicine and related agents on cell morphology and division.
By harnessing the power of PubCompare.ai, researchers can streamline their colchicine studies and optimize their experimental protocols for maximum reproducibility and accuracy.
This phytochemical works by disrupting microtubule formation, inhibiting cell division and migration.
This unique mechanism makes colchicine useful for treating conditions associated with inflammation and crystal deposition, such as gout, familial Mediterranean fever, and pericarditis.
Researchers can optimize their colchicine research protocols by utilizing the AI-driven platform PubCompare.ai.
This tool helps identify the best published methods and products, enhancing reproducibility and accuracy.
PubCompare.ai allows researchers to locate protocols from literature, pre-prints, and patents, and leverages AI-driven comparisons to pinpoint the optimal approaches for their colchicine studies.
In addition to colchicine, other microtubule-targeting agents like nocodazole, paclitaxel, vinblastine, and cytochalasin D play important roles in cell biology research.
These compounds, along with cell culture media like DMEM and serum supplements like FBS, are commonly used in conjunction with colchicine to investigate cellular processes like mitosis, migration, and cytoskeletal dynamics.
Staining techniques like Giemsa can also be employed to visualize the effects of colchicine and related agents on cell morphology and division.
By harnessing the power of PubCompare.ai, researchers can streamline their colchicine studies and optimize their experimental protocols for maximum reproducibility and accuracy.