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Colforsin

Colforsin is a forskolin derivative that acts as a potent and selective adenylate cyclase activator, leading to increased intracellular levels of cyclic AMP.
It has been studied for its potential therapeutic applications in various diseases, including asthma, heart failure, and neurological disorders.
Colforsin's mechanism of action involves the stimulation of adenylate cyclase, an enzyme responsible for the conversion of ATP to cAMP, a key second messenger in cellular signaling pathways.
This action can lead to the modulation of various physiological processes, such as smooth muscle relaxation, cardiovascular function, and neuronal excitability.
Researches are actively investigating the use of Colforsin and its analogs as pharmacological tools and potential therapeutic agents.

Most cited protocols related to «Colforsin»

1
FRET Imaging of cAMP Signaling
Cited 28 times
Cells grown on coverslips were placed on a temperature-controlled (37° C) inverted Nikon Diaphot microscope and excited at 425 nm. Donor and acceptor emission was detected simultaneously with two photomultipliers, using a 505 nm beamsplitter and optical filters: 470 ± 20 nm (CFP) and 530 ± 25 nm (YFP). Signals were digitized and FRET was expressed as the ratio between donor and acceptor signals. The FRET ratio was set at 1 at the onset of the experiment. Cells were stimulated with 25 μM forskolin and 100 μM IBMX to maximally raise the cAMP levels. Data from a minimum of 15 cells over three experiments are presented as mean ± s.e.m.
Klarenbeek J., Goedhart J., van Batenburg A., Groenewald D, & Jalink K. (2015). Fourth-Generation Epac-Based FRET Sensors for cAMP Feature Exceptional Brightness, Photostability and Dynamic Range: Characterization of Dedicated Sensors for FLIM, for Ratiometry and with High Affinity. PLoS ONE, 10(4), e0122513.
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Publication 2015
1-Methyl-3-isobutylxanthine Cells Colforsin Fluorescence Resonance Energy Transfer Microscopy Tissue Donors
2
Rolipram Modulates Sleep Deprivation-Induced Memory
Cited 26 times
C57BL/6J male mice (2–5 months of age) were housed individually on a 12 hr/12 hr light/dark schedule with lights on at 7 A.M. (ZT0) and handled for 6 days. Mice were sleep-deprived (SD) in their home cages for 5 hours by gentle handling beginning at ZT5 or left undisturbed (non-sleep-deprived mice, NSD). For contextual fear conditioning experiments, animals were placed in a novel chamber for 3 minutes, and received a 2-second, 1.5 mA footshock after 2.5 minutes. Half of the mice were deprived of sleep for 5 hours post-training. Mice received intra-peritoneal injections of rolipram (ROL; 1 mg/kg) or vehicle (2% DMSO in 0.9% saline) immediately and 2.5 hours post-training. Testing of contextual memory was performed 24 hours after training in the trained context and 48 hours after training in a novel chamber.
Electrophysiological recordings were carried out as previously reported28 (link). 1-train LTP was induced by a single 100 Hz, 1-second duration train of stimuli. 4-train LTP consisted of 4 trains applied with a 5-minute inter-train interval; for massed 4-train LTP a 5-second inter-train interval was used. Theta-burst stimulation (TBS) consisted of 40-ms duration, 100 Hz bursts delivered at 5 Hz for 3 seconds (15 bursts of 4 pulses per burst, for a total of 60 pulses). Chemical LTP was induced by treatment of slices for 15 minutes with 5µM forskolin (FSK) in 0.1% ethanol, or a combination of 50µM forskolin and 30µM 3-isobutyl-1-methylxanthine (IBMX, in water). Rolipram (0.1µM in 0.1% DMSO) was applied for 60 minutes, beginning 30 minutes before tetanization.
cAMP assays on CA1 regions of hippocampal slices 10 minutes after treatment for 15 minutes with forskolin (50µM), forskolin + IBMX (30µM), or vehicle (0.1% EtOH) were performed by radioimmunoassay according to kit instructions. cAMP-specific PDE activity assays29 (link) and Western blots for PDE4A530 (link) were performed as previously described.
Full Methods and any associated references are available in the online version of the paper at www.nature.com/nature.
Vecsey C.G., Baillie G.S., Jaganath D., Havekes R., Daniels A., Wimmer M., Huang T., Brown K.M., Li X.Y., Descalzi G., Kim S.S., Chen T., Shang Y.Z., Zhuo M., Houslay M.D, & Abel T. (2009). Sleep deprivation impairs cAMP signaling in the hippocampus. Nature, 461(7267), 1122-1125.
Publication 2009
1-Methyl-3-isobutylxanthine Aftercare Animals Biological Assay CA1 Field of Hippocampus Colforsin Ethanol Fear Injections, Intraperitoneal Light Males Memory methylxanthine Mice, House Mice, Inbred C57BL Neoplasm Metastasis Normal Saline Pulses Radioimmunoassay Rolipram Sleep Sulfoxide, Dimethyl Western Blot
3
FRET Imaging of cAMP Signaling
Cited 19 times
Cells on coverslips were placed on a thermostatted (37°C) inverted Nikon Diaphot microscope and excited at 425 nm. Donor and acceptor emission was detected simultaneously with two photomultipliers, using a 505 nm beamsplitter and optical filters: 470±20 nm (CFP) and 530±25 nm (YFP). Signals were digitized and FRET was expressed as the ratio between donor and acceptor signals. The FRET value was set at 1 at the onset of the experiment. Cells were stimulated with 25 µM forskolin and 100 µM IBMX to maximally raise the cAMP levels. Data from 6–15 cells per experiment are presented as mean ± s.e.m.
Klarenbeek J.B., Goedhart J., Hink M.A., Gadella T.W, & Jalink K. (2011). A mTurquoise-Based cAMP Sensor for Both FLIM and Ratiometric Read-Out Has Improved Dynamic Range. PLoS ONE, 6(4), e19170.
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Publication 2011
1-Methyl-3-isobutylxanthine Cells Colforsin Fluorescence Resonance Energy Transfer Microscopy Tissue Donors
4
Adipocyte Differentiation and Thermogenic Activation
Cited 16 times
Immortalized progenitor cells were plated and grown in DMEM/H medium supplemented with 10% FBS (referred as day 0). For adipocyte differentiation, cell were grown for 6 days until reaching confluence (day 6), and then treated with the adipogenic induction medium as described above for 12 days (day 18). To further stimulate thermogenic program, fully differentiated cells were incubated with 10μM forskolin or 1 μM norepinephrine for 4 h. For BMPs and FGF21 pre-treatment, recombinant BMP7 (3.3 nM), BMP8 (3.3 nM), or FGF21 (50 nM) were added to undifferentiated cells in medium containing insulin (0.5 μM), T3 (2 nM) and 2% FBS for 6 days followed by adipogenic induction for 12 days. For BMPs and FGF21 post-treatment, fully differentiated adipocytes at day 18 were treated with recombinant BMP7 (3.3 nM), BMP8 (3.3 nM), or FGF21 (50 nM) in medium containing insulin (0.5 μM), T3 (2 nM) and 2% FBS for 2 days. We routinely check for mycoplasma contamination and all the cells used in this study are free of mycoplasma.
Xue R., Lynes M.D., Dreyfuss J.M., Shamsi F., Schulz T.J., Zhang H., Huang T.L., Townsend K.L., Li Y., Takahashi H., Weiner L.S., White A.P., Lynes M.S., Rubin L.L., Goodyear L.J., Cypess A.M, & Tseng Y.H. (2015). Clonal analyses and gene profiling identify genetic biomarkers of human brown and white preadipocyte thermogenic potential. Nature medicine, 21(7), 760-768.
Publication 2015
Adipocytes Adipogenesis Bone Morphogenetic Protein 7 Bone Morphogenetic Proteins Cells Colforsin fibroblast growth factor 21 Insulin Mycoplasma Norepinephrine Pancreatic beta Cells Stem Cells Thermogenesis
5
Measuring Adipocyte Lipolysis
Cited 16 times

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Publication 2014
Adipocytes Adrenergic Agents Cells Colforsin Glycerin Isoproterenol Lipolysis

Most recents protocols related to «Colforsin»

1
Establishment and Characterization of PDTO
Tumor pieces will be dissociated enzymatically and mechanically to obtain isolated cells or small cell clusters (Fig. 2). Cells will be embedded in an extracellular matrix (growth factor-reduced Matrigel or BME II) and cultured in a medium supplemented with growth factors and signal pathway inhibitors [Advanced DMEM (Gibco) supplemented with 100 UI/mL of penicillin and streptomycin (Gibco), 1% GlutaMAX (Gibco), 1X B27 (Gibco), 1.25 mM NAC (Sigma-Aldrich), 50 ng/mL EGF (PeproTech), 10 ng/mL FGF-10 (PeproTech), 5 ng/mL FGF-b (PeproTech), 500 nM A-83-01 (PeproTech), 10 μM Y27632 (Interchim), 10 mM Nicotinamide (Sigma-Aldrich), 1 μM PGE2 (PeproTech), 1 μM Forskolin (Peprotech), 0.3 μM CHIR99021 (Biogems), 100 μg/mL Primocin (InvivoGen), 50% Wnt3a, RSPO3, Noggin-conditioned media (L-WRN, ATCC), and 10% RSPO1-conditioned media (Cultrex HA-R-Spondin-1-Fc 293 T, Amsbio)]. Culture medium will be changed twice a week. Once formed, PDTO will be dissociated and reseeded to amplify them for experimental purposes. Cryovials will be prepared at regular intervals by dissociating and resuspending PDTO in Recovery Cell Culture Freezing Medium (Gibco) prior to be biobanked in liquid nitrogen. It should be noted that PDTO line will be considered as established when it will be maintained for more than 3 passages. For each PDTO line, samples will be kept frozen for DNA/RNA/protein analysis and others will be embedded in paraffin for histopathological analysis. This will allow comparisons between the characteristics of the PDTO and the tumor from which they are derived in order to validate their correspondence.

Establishment and characterization of PDTO derived from HNSCC and evaluation of response to treatments to assess its predictive value

Perréard M., Florent R., Divoux J., Grellard J.M., Lequesne J., Briand M., Clarisse B., Rousseau N., Lebreton E., Dubois B., Harter V., Lasne-Cardon A., Drouet J., Johnson A., Le Page A.L., Bazille C., Jeanne C., Figeac M., Goardon N., Vaur D., Micault E., Humbert M., Thariat J., Babin E., Poulain L., Weiswald L.B, & Bastit V. (2023). ORGAVADS: establishment of tumor organoids from head and neck squamous cell carcinoma to assess their response to innovative therapies. BMC Cancer, 23, 223.
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Publication 2023
Cell Culture Techniques Cells Chir 99021 Colforsin Culture Media Culture Media, Conditioned Dinoprostone Extracellular Matrix Freezing Growth Factor HSP40 Heat-Shock Proteins inhibitors matrigel Neoplasms Niacinamide Nitrogen noggin protein Paraffin Embedding Penicillins Signal Pathways Squamous Cell Carcinoma of the Head and Neck Streptomycin Y 27632
2
Assaying ATP and cAMP Levels in Cells
1 x 104 293T cells were seeded in white μclear 96-well culture plates and 1.4*105 cells were seeded in 12-well culture plates. After 24 h cells were incubated with increasing concentrations of ATP-depleting compounds Antimycin A (Sigma) and 2-deoxy-D-glucose (Sigma) for 30 min at 37°C. ATP levels were determined with the CellTiter-Glo 2.0 assay (Promega) as per the manufacturer’s instructions. Luminescence was measured in a Mithras LB 940 Multilabel reader (Berthold Technologies). Cells were lysed in 4× LSB for WB analysis. An SFV MOI 10 sample 6 h p.i. was included as a positive control.
1 x 104 Vero E6 cells were seeded in white μclear 96-well culture plates. After 24 h the cells were incubated with a 2-fold dilution of the cAMP increasing compound forskolin (Cayman chemical) for 30 min at 37°C and cAMP levels were determined with the cAMP-Glo assay (Promega) as per the manufacturer’s instructions. Luminescence was measured in a Mithras LB 940 Multilabel reader (Berthold Technologies). 1.4*105 cells were seeded in 12-well culture plates. After 24 h cells were incubated with a 2-fold dilution of cAMP increasing compounds forskolin. For the cAMP-Glo Assay forskolin had to be diluted in an induction buffer containing two phosphodiesterase inhibitors to prevent cAMP hydrolysis; 500 μM isobutyl-1-methylxanthine (IBMX) (Sigma) and 100 μM Ro 20–1724 (Sigma). Graphpad Prism 8 was used to do a two-tailed non-paired t-tests to determine significant differences. For the WB experiment the 2-fold dilution was started with 5 μM forskolin, 500 μM IBMX and 100 μM Ro 20–1724. Cells were lysed in 4× LSB for WB analysis. An SFV MOI 10 sample 6 h p.i. was included as a positive control.
Treffers E.E., Tas A., Scholte F.E., de Ru A.H., Snijder E.J., van Veelen P.A, & van Hemert M.J. (2023). The alphavirus nonstructural protein 2 NTPase induces a host translational shut-off through phosphorylation of eEF2 via cAMP-PKA-eEF2K signaling. PLOS Pathogens, 19(2), e1011179.
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Publication 2023
4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone Antimycin A Biological Assay Buffers Caimans Cells Colforsin Glucose HEK293 Cells Hydrolysis Luminescence methylxanthine oxytocin, 1-desamino-(O-Et-Tyr)(2)- Phosphodiesterase Inhibitors prisma Promega Technique, Dilution Vero Cells
3
Differentiation of hiPSCs into Endothelial Cells
We differentiated hiPSCs into ECs according to the protocol previously described by Patsch et al. (36 (link)) and then adapted for differentiation in 3D culture. In brief, hiPSCs were dissociated from 10-cm plates using ReLeSR (StemCell Technologies) according to the manufacturer’s instructions. Cells were seeded into 125-ml Erlenmeyer flasks at 200,000 cells/ml in 20 ml of StemScale media (Thermo Fisher Scientific) containing 10 μM Y-27632 and then placed on a plate shaker rotating at 70 rpm. Media were changed to fresh StemScale media the next day to remove the ROCK inhibitor. EC differentiation began 4 days after seeding, when spheroids reached approximately 300 μm in diameter. On day 0 of differentiation, media were changed to N2B27 medium containing 7 μM CHIR99021 and BMP-4 (25 μg/ml) and incubated for 3 days without media change. On day 3 of differentiation, media were changed to StemPro-34 containing GlutaMAX (Thermo Fisher Scientific) with 2 μM forskolin (Selleck Chemicals) and VEGF (200 ng/ml) (PeproTech). This same media composition with StemPro-34, forskolin, and VEGF was changed fresh daily for 3 days through day 5 of differentiation. On day 6 of differentiation, spheroids were dissociated using 0.5% trypsin-EDTA diluted 1:3 in phosphate-buffered saline (PBS) for ~5 min. CD144+ ECs were sorted using magnetic microbeads using magnetic-activated cell sorting columns (Miltenyi Biotec) according to the manufacturer’s instructions. Sorted CD144+ ECs were replated on Geltrex-coated 10-cm plates and allowed to proliferate for 3 to 7 days in StemPro-34 containing GlutaMAX and VEGF (50 ng/ml) and then redissociated with trypsin-EDTA and replated again onto Geltrex-coated plates for assay.
Lin Z., Garbern J.C., Liu R., Li Q., Mancheño Juncosa E., Elwell H.L., Sokol M., Aoyama J., Deumer U.S., Hsiao E., Sheng H., Lee R.T, & Liu J. (2023). Tissue-embedded stretchable nanoelectronics reveal endothelial cell–mediated electrical maturation of human 3D cardiac microtissues. Science Advances, 9(10), eade8513.
Publication 2023
Biological Assay BMP4 protein, human Cells Chir 99021 Colforsin Edetic Acid Human Induced Pluripotent Stem Cells Microspheres Phosphates Saline Solution Stem Cells Trypsin Vascular Endothelial Growth Factors Y 27632
4
In Vitro Kisspeptin Effects on Steroid Production
Kisspeptin-10 (rat) and kisspeptin antagonist (p234) were purchased from Tocris Bioscience (Abingdon, Oxon, UK). Kisspeptin-10 corresponds to the C-terminal region of the translated kisspeptin 54 peptide (residues 112–121), and its sequence is identical in rat and bovine. Kisspeptin-10 and p234 (antagonist) were dissolved in water and 20% (w/v) acetonitrile in water, respectively, to give a stock concentration of 10–3 M. These were further diluted in sterile medium to give final concentrations of 10–6, 10–7, 10–8, 10–9 and 10–10 M. In each culture model, the effects of kisspeptin-10 and kisspeptin antagonist on steroid production and viable cell number were evaluated under both basal and gonadotrophin or forskolin (FSK)-stimulated conditions as explained below. Highly purified ovine FSH (oFSH 19SIAPP) and LH (oLH-S-16) were provided by the NHPP (Torrance, CA, USA). In NLTC cultures, LH was used at a final concentration of 150 pg/mL, shown previously to elicit maximal A4 secretion (Glister et al. 2005 (link)); A4 and P4 secretion were evaluated. In NLGC cultures, FSH was used at a final concentration (0.3 ng/mL) shown previously to elicit optimal E2 secretion (Glister et al. 2001 (link), 2004 (link)); E2 and P4 secretion were evaluated. As LGC and LTC are largely unresponsive to gonadotrophin stimulation in this serum-supplemented culture model (Kayani et al. 2009 (link)), the adenylate cyclase activator, forskolin (FSK; 10 μM), was used as an alternative secretagogue; only P4 secretion was evaluated for these cells since A4 and E2 secretion are both extremely low.
Mattar D., Cheewasopit W., Samir M, & Knight P.G. (2023). Does kisspeptin exert a local modulatory effect on bovine ovarian steroidogenesis?. Reproduction & Fertility, 4(1), e220088.
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Publication 2023
acetonitrile Adenylate Cyclase Bos taurus Cells Colforsin Gonadotropins KISS1 protein, human kisspeptin-10 Metastin paragloboside polypeptide C potassium peroxymonosulfuric acid Secretagogues secretion Serum Sheep Sterility, Reproductive Steroids
5
Isolation and Characterization of Murine Fibroblastic Reticular Cells
FRC cell lines were established from peripheral LNs by long-term culturing as described previously74 (link) with minor modifications. In brief, LNs from 8-week-old C57BL/6 N mice were dissected and disrupted using two 25 G needles before enzymatic digestion with DMEM medium containing 3.5 mg/ml Collagenase D and 40 μg/ml DNase I at 37 °C for 30 min with agitation75 . The mixture was then filtered through a 70 μm cell strainer and centrifuged at 300 g for 5 min at 4 °C. The cell pellet was resuspended and cultured in DMEM medium (supplemented with 10% FBS and 1% Penicillin/Streptomycin) (5% CO2, 37 °C). After 24 h, non-adherent cells were removed, and fresh medium was added to continue culturing until cells reached confluence. Adherent-stromal cells were then trypsinized, and triple-stained with antibodies to identify FRCs: Cd45 Pacific blue (1:100), Cd31 PE (1:100) and gp38 APC (1:100). FRCs were sort-purified using a MoFlo Optical Bench Sorter (Beckman Coulter) to achieve a purity of ≥95%. The sorted cells were immediately cultured in DMEM medium for expansion, and then seeded into 60 mm dishes in a density of 3 × 105 per well to grow until confluence, followed by starvation for overnight and treatment with 10 μM isoproterenol (Sigma-Aldrich), 5 μM forskolin (Sigma-Aldrich), and 5 μM PKA inhibitor H89 dihydrochloride hydrate (Sigma-Aldrich) for 8 h. Culture media were collected to determine the concentration of IL-33 using a mouse IL-33 immunoassay kit (Immunodiagnostics Limited) or LDH using a CyQUANT LDH Cytotoxicity fluorescent assay kit (Thermo Fisher Scientific).
Cheong L.Y., Wang B., Wang Q., Jin L., Kwok K.H., Wu X., Shu L., Lin H., Chung S.K., Cheng K.K., Hoo R.L, & Xu A. (2023). Fibroblastic reticular cells in lymph node potentiate white adipose tissue beiging through neuro-immune crosstalk in male mice. Nature Communications, 14, 1213.
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Publication 2023
Antibodies Biological Assay Cell Lines Cells Colforsin Collagenase Culture Media Cytotoxin Deoxyribonuclease I Digestion Enzymes Hyperostosis, Diffuse Idiopathic Skeletal IL33 protein, human Immunoassay Immunodiagnosis Isoproterenol Mice, Inbred C57BL Mus Needles Penicillins PKA inhibitor Streptomycin Stromal Cells Vision

Top products related to «Colforsin»

1
Forskolin by Merck Group
Sourced in United States, Germany, United Kingdom, France, Sao Tome and Principe, Canada, Italy, Japan, China, Switzerland, Macao, Australia
Forskolin is a lab equipment product manufactured by Merck Group. It is a compound derived from the roots of the Coleus forskohlii plant. Forskolin is used as a tool for research purposes in the laboratory setting.
2
Forskolin by Bio-Techne
Sourced in United Kingdom, United States, Germany
Forskolin is a compound extracted from the roots of the Coleus forskohlii plant. It is a cyclic adenosine monophosphate (cAMP) activator, which can be used as a research tool in cell-based assays and in vitro studies.
3
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
4
Dimethyl sulfoxide (dmso) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
5
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
6
3 isobutyl 1 methyl xanthine (ibmx) by Merck Group
Sourced in United States, United Kingdom, Germany, France, China, Denmark, Japan, New Zealand, Macao, Sweden, Spain
IBMX is a laboratory product manufactured by Merck Group. It is a chemical compound that functions as a phosphodiesterase inhibitor. The core function of IBMX is to inhibit the activity of phosphodiesterase enzymes, which are involved in the regulation of cellular processes. This product is intended for use in research and laboratory settings.
7
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
8
Forskolin fsk by Merck Group
Sourced in United States, United Kingdom, Italy
Forskolin (FSK) is a chemical compound extracted from the root of the Coleus forskohlii plant. It is a labeling reagent commonly used in scientific research and laboratory settings. Forskolin functions as an activator of the enzyme adenylate cyclase, which plays a role in the regulation of cellular processes. The core function of Forskolin is to facilitate the study of cellular signaling pathways and related biological mechanisms.
9
3 isobutyl 1 methylxanthine by Merck Group
Sourced in United States, Germany, United Kingdom, China, Japan, Italy, Sao Tome and Principe, India, France, Macao, Austria, Sweden, Australia
3-isobutyl-1-methylxanthine is a chemical compound primarily used as a research tool in laboratories. It functions as a nonselective phosphodiesterase inhibitor, which can affect various cellular processes. The core function of this product is to serve as a laboratory reagent for scientific research purposes.
10
Forskolin by Cayman Chemical
Sourced in United States
Forskolin is a chemical compound isolated from the Indian Coleus plant. It is a labeling standard used for identification and quantification purposes in analytical procedures.

More about "Colforsin"

Colforsin, a potent and selective adenylate cyclase activator, is a forskolin derivative that has garnered significant interest in the scientific community.
This compound acts by stimulating the enzyme adenylate cyclase, which is responsible for the conversion of ATP to cAMP, a crucial second messenger in cellular signaling pathways.
This mechanism of action can lead to the modulation of various physiological processes, such as smooth muscle relaxation, cardiovascular function, and neuronal excitability, making it a potential therapeutic agent for conditions like asthma, heart failure, and neurological disorders.
Forskolin, the parent compound of Colforsin, is a natural product derived from the roots of the Coleus forskohlii plant.
It has been studied extensively for its ability to activate adenylate cyclase and increase intracellular cAMP levels.
Forskolin and its analogs, such as Colforsin, have been employed as pharmacological tools to investigate the role of cAMP in cellular signaling and as potential therapeutic agents.
When conducting research on Colforsin, it is often necessary to use cell culture techniques, which may involve the use of fetal bovine serum (FBS), dimethyl sulfoxide (DMSO), penicillin/streptomycin, and 3-isobutyl-1-methylxanthine (IBMX), a phosphodiesterase inhibitor that can further enhance cAMP levels.
Additionally, Dulbecco's Modified Eagle Medium (DMEM) is a commonly used cell culture medium that provides the necessary nutrients and growth factors for cell proliferation and maintenance.
By leveraging the insights gained from the MeSH term description and the Metadescription, researchers can optimize their Colforsin studies by accessing relevant protocols, comparing different approaches, and enhancing the reproducibility and accuracy of their experiments.
PubCompare.ai's AI-powered platform can be a valuable resource in this endeavor, providing a comprehensive overview of the latest research and facilitating the identification of the most effective protocols and products.