Human breast epithelial cells (MCF10A) were transfected with a pQCXIH vector and were cultured in DMEM/F12 (Invitrogen, Carlsbad, CA) supplemented with 5% horse serum (Invitrogen), 20 ng/mL EGF, 0.5 μg/mL hydro-cortisone, 100 ng/mL cholera toxin, 10 μg/mL insulin, and 50 μg/mL penicillin/streptomycin until 70–80% confluence was reached. The cells were then lysed in a buffer containing 8 M urea, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 50 mM ammonium bicarbonate, one-third tablet of protease inhibitor, and 2 mM sodium ortho-vanadate. Proteins were denatured, reduced, and alkylated after which tryptic digestions were performed at an enzyme/substrate ratio of 1:50. For method comparison between SCX and RP, digested peptides were cleaned by flowing through a 1 mL solid-phase extraction C18 column (Discovery DSC-18, SUPELCO, Bellefonte, PA). Samples were concentrated using a Speed-Vac SC 250 Express (Thermo Savant, Holbrook, NY) and stored at −80°C until time for analysis. A 300.0 μg desalted peptide sample was used for each SCX, low-pH RPLC, and high-pH RPLC fractionation. A 300.0 μg nondesalted protein digest was used to evaluate the potential of high-pH approach for desalting.
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Organic Chemical
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Cortisone
Cortisone
Cortisone is a potent glucocorticoid hormone that plays a crucial role in the regulation of metabolism, immune function, and inflammation.
It is naturally produced by the adrenal gland and has been widely used in medical treatments for a variety of conditions, including asthma, arthritis, and autoimmune disorders.
Cortisone exerts its effects by binding to glucocorticoid receptors, which then modulate the expression of target genes involved in these physiological processes.
Reserach on the optimal use of cortisone-based therapies continues to evolve, with new protocols and prodcuts being developed to enhance effeciency and minimize side effects.
PubCompare.ai offers a cutting-edge platform to help researchers idnetify the best cortisone-related protocols from the latest literature, preprints, and patents, supporting data-driven decision making and advancements in this important area of biomedical science.
It is naturally produced by the adrenal gland and has been widely used in medical treatments for a variety of conditions, including asthma, arthritis, and autoimmune disorders.
Cortisone exerts its effects by binding to glucocorticoid receptors, which then modulate the expression of target genes involved in these physiological processes.
Reserach on the optimal use of cortisone-based therapies continues to evolve, with new protocols and prodcuts being developed to enhance effeciency and minimize side effects.
PubCompare.ai offers a cutting-edge platform to help researchers idnetify the best cortisone-related protocols from the latest literature, preprints, and patents, supporting data-driven decision making and advancements in this important area of biomedical science.
Most cited protocols related to «Cortisone»
ammonium bicarbonate
beta-glycerol phosphate
Breast
Buffers
Cells
Cholera Toxin
Cloning Vectors
Cortisone
Digestion
Enzymes
Epithelial Cells
Equus caballus
Fractionation, Chemical
Homo sapiens
Insulin
Penicillins
Peptides
Protease Inhibitors
Proteins
Serum
sodium pyrophosphate
Sodium Vanadate
Solid Phase Extraction
Streptomycin
Tablet
Trypsin
Urea
Antidepressive Agents
beta-Arrestin 2
Brain
Corticosterone
Cortisone
Cyclodextrins
Fluoxetine
Fluoxetine Hydrochloride
Hypromellose
Imipramine Hydrochloride
Light
Mice, House
Reboxetine
Tube Feeding
Animals, Laboratory
Cortisone
Europeans
Food
Institutional Animal Care and Use Committees
Light
Males
Mice, House
Mice, Inbred C57BL
Rivers
To generate MNPs, hPSCs were dissociated with Dispase (1 mg/ml) and split 1:6 on irradiated MEFs or Matrigel coated plates. On the following day, the PSC medium was replaced with a chemically defined neural medium, including DMEM/F12, Neurobasal medium at 1:1, 0.5×N2, 0.5×B27, 0.1mM ascorbic acid (Santa Cruz), 1×Glutamax and 1×penicillin/streptomycin (All others from Invitrogen). CHIR99021 (3uM, Torcris), 2μM DMH-1 (Torcris) and 2μM SB431542 (Stemgent) were added in the medium. The culture medium was changed every other day. Human PSCs maintained under this condition for 6 days were induced into NEP cells. The NEP cells were then dissociated with Dispase (1 mg/ml) and split at 1:6 with the same medium described above. RA (0.1μM, Stemgent) and 0.5μM Purmorphamine (Stemgent) were added in combination with 1μM CHIR99021, 2μM DMH-1, and 2μM SB431542. The medium was changed every other day. NEP cells maintained under this condition for 6 days differentiated into OLIG2+ MNPs. The OLIG2+ MNPs were expanded with the same medium containing 3μM CHIR99021, 2μM DMH-1, 2μM SB431542, 0.1μM RA, 0.5μM Purmorphamine and 0.5 mM VPA (Stemgent), and split 1:6 once a week with Dispase (1 mg/ml). OLIG2+ MNPs were frozen with the regular frozen medium (DMEM/F12, 10% fetal bovine serum and 10% DMSO) in liquid nitrogen, and cultured again in expansion medium after thawing.
To induce MN differentiation, OLIG2+ MNPs were dissociated with Dispase (1 mg/ml) and cultured in suspension in the above neural medium with 0.5μM RA and 0.1μM Purmorphamine. The medium was changed every other day. OLIG2+ MNPs under this condition for 6 days differentiated into MNX1+ MNs. The MNX1+ MNs were then dissociated with Accumax (eBioscience) into single cells and plated on Matrigel coated plates or on astrocytes. The MNX1+ MNs were cultured with 0.5μM RA, 0.1μM Purmorphamine and 0.1μM Compound E (Calbiochem) for 10 days to mature into CHAT+ MNs. Insulin-like growth factor 1(IGF-1), brain-derived neurotrophic factor (BDNF), and ciliary neurotrophic factor (CNTF) (all from R&D, 10 ng/ml each) were added if MNs were plated at low density. For identifying MN disease phenotypes, SMA and ALS MNs were cultured without these neurotrophic factors.
To induce MN differentiation, OLIG2+ MNPs were dissociated with Dispase (1 mg/ml) and cultured in suspension in the above neural medium with 0.5μM RA and 0.1μM Purmorphamine. The medium was changed every other day. OLIG2+ MNPs under this condition for 6 days differentiated into MNX1+ MNs. The MNX1+ MNs were then dissociated with Accumax (eBioscience) into single cells and plated on Matrigel coated plates or on astrocytes. The MNX1+ MNs were cultured with 0.5μM RA, 0.1μM Purmorphamine and 0.1μM Compound E (Calbiochem) for 10 days to mature into CHAT+ MNs. Insulin-like growth factor 1(IGF-1), brain-derived neurotrophic factor (BDNF), and ciliary neurotrophic factor (CNTF) (all from R&D, 10 ng/ml each) were added if MNs were plated at low density. For identifying MN disease phenotypes, SMA and ALS MNs were cultured without these neurotrophic factors.
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide
Ascorbic Acid
Astrocytes
Cells
Chir 99021
Ciliary Neurotrophic Factor
Cortisone
dispase
Fetal Bovine Serum
Freezing
Homo sapiens
IGF1 protein, human
matrigel
Nerve Growth Factors
Nervousness
Neurotrophic Factor, Brain-Derived
Nitrogen
OLIG2 protein, human
Pancreatic Stellate Cells
Penicillins
Phenotype
purmorphamine
Streptomycin
Sulfoxide, Dimethyl
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide
Ascorbic Acid
Astrocytes
Cells
Chir 99021
Ciliary Neurotrophic Factor
Cortisone
dispase
Fetal Bovine Serum
Freezing
Homo sapiens
IGF1 protein, human
matrigel
Nerve Growth Factors
Nervousness
Neurotrophic Factor, Brain-Derived
Nitrogen
OLIG2 protein, human
Pancreatic Stellate Cells
Penicillins
Phenotype
purmorphamine
Streptomycin
Sulfoxide, Dimethyl
Most recents protocols related to «Cortisone»
Example 8
In this example, research shows that during the mass spectrometric detection of the 5 markers, the cationic mode should be chosen to detect angiotensin I, angiotensin II, cortisol and 18-hydrocorticosterone, while the anionic mode needs to be chosen to detect aldosterone; this is because when the cationic mode is chosen, there exists a peak diagram of cortisone, an isomer of aldosterone, nearby the detection peak of aldosterone to cause larger interference, and CV % is greater than 15%; but when the anionic mode is applied for detection, the test result is more stable and accurate, and CV% is less than 8.33%.
18-Hydroxycorticosterone
Aldosterone
Angiotensin I
Angiotensin II
Cations
Cortisone
Hydrocortisone
Isomerism
Mass Spectrometry
Example 107
The analgesic efficacy of the compounds disclosed in the present application, for example, compound 3, 6B, 10, 11, 53, 56, 59, or 62, may be assessed in the post-incision model in rats. Rats may be anesthetized and receive an incision in one hindpaw. The following day, rats may be administered test compound (e.g., compound 3, 6B, 10, 11, 53, 56, 59, or 62) by a systemic route of administration (e.g., oral gavage, subcutaneous injection, intravenous, etc.) to achieve appropriate plasma exposure. Between 30 and 120 min later, mechanical allodynia may be assessed using the Up-down method with von Frey hairs (Chaplan, S. R., Bach, F. W., Pogrel, J. W., Chung, J. M. & Yaksh, T. L. Quantitative assessment of tactile allodynia in the rat paw. J Neurosci Meth 53, 55-63 (1994). Rats may be stimulated with the hair in the middle of the series (for example, 2.0 g) and consequent stimuli may be presented in consecutive order, either ascending or descending. A paw withdrawal response to the hair may result in presentation of the next weaker stimulus; absence of a paw withdrawal response may result in presentation of the next stronger stimulus. Administration of the compound may result in increased threshold for von Frey hair stimulation to induce paw withdrawal i.e. decreased mechanical allodynia.
Analgesics
Cortisone
Hair
Mechanical Allodynia
Methamphetamine
Pain, Postoperative
Plasma
Rattus norvegicus
Subcutaneous Injections
Tactile Allodynia
Tube Feeding
All patients were examined by their surgeon, with particular attention to the need for sedation according to the patient’s history and psychological profile. The indication for surgery was phacoemulsification with the placement of an implant in the capsular bag. Intracameral cefuroxime antibiotic prophylaxis was administered systematically in both groups. Postoperative treatment included a topical antibiotic (1 week), a non-steroidal anti-inflammatory (1 month), and cortisone antibiotic eye drops (1 month). Patients in the Surgicube® group underwent topical anesthesia with tetracaine or oxybuprocaine, followed by additional intracameral lidocaine. They were prepared by the nurse and installed on a stretcher, with only the head arriving at the level of the sterile operating area by the laminar air flow. There was no anesthetist or peripheral venous line. The control group had topical anesthesia followed by an intracameral lidocaine injection. An anesthetist nurse was always present in the room. Patients had to change into an overall.
Anesthetist
Anti-Inflammatory Agents, Non-Steroidal
Antibiotic Prophylaxis
Antibiotics
Attention
benoxinate
Capsule
Cefuroxime
Cortisone
Eye Drops
Head
Lidocaine
Nurses
Operative Surgical Procedures
Patient Holding Stretchers
Patients
Phacoemulsification
Sedatives
Sterility, Reproductive
Surgeons
Tetracaine
Topical Anesthetics
Veins
Protocol full text hidden due to copyright restrictions
Open the protocol to access the free full text link
ARID1A protein, human
Aves
Biological Assay
BLOOD
Chickens
Cortisone
Enzyme-Linked Immunosorbent Assay
Myoglobin
Serum
Sterility, Reproductive
Troponin
Troponin I
Veins
Data with normal distribution are expressed as mean ± SD, whereas data with abnormal distribution are expressed as median (interquartile range [IQR]). We employed a paired t-test to compare changes in parameters during and after the gout flare. The differences between the groups were tested for statistical significance by an independent sample t-test. The Mann–Whitney U non-parametric test was employed for variables that were not normally distributed. We calculated Pearson’s correlation coefficient to evaluate the relationship between parameters. We tested the association between SUA and the levels of CORT, IL-1β, IL-6, and TNF-α using the same model. Further, we applied linear mixed effect models to estimate the association between the percent changes in 24 h Uur and 24 h UFC. Model 1 required no adjustment. Model 2 was adjusted for the percent changes in IL-1β, IL-6, and TNF-α. Model 3 was further adjusted for the percent changes in CORT, 24 h FEur, and 24 h EurGF. The statistical significance was set at p < 0.05, as indicated (two-tailed test). SPSS v.26.0 (IBM SPSS, Chicago, IL, USA) was used for data analysis.
Cortisone
Gout
Interleukin-1 beta
Tumor Necrosis Factor-alpha
Top products related to «Cortisone»
Sourced in United States, Germany, United Kingdom, Switzerland, Japan, Macao, Italy
Cortisone is a laboratory equipment product manufactured by Merck Group. It is a corticosteroid compound used in various scientific and research applications.
Sourced in Germany, United States
Compound E is a laboratory instrument designed for the analysis and identification of chemical compounds. It utilizes advanced spectroscopic techniques to provide detailed information about the molecular structure and composition of samples. The core function of Compound E is to enable precise and accurate analysis of a wide range of chemical substances, supporting research, development, and quality control activities in various industries.
Sourced in United States, Germany, United Kingdom, China, Japan, Italy, Sao Tome and Principe, Macao, France, Australia, Switzerland, Canada, Denmark, Spain, Israel, Belgium, Ireland, Morocco, Brazil, Netherlands, Sweden, New Zealand, Austria, Czechia, Senegal, Poland, India, Portugal
Dexamethasone is a synthetic glucocorticoid medication used in a variety of medical applications. It is primarily used as an anti-inflammatory and immunosuppressant agent.
Sourced in United States, Germany, United Kingdom, Switzerland, Japan, Macao, Sweden, Italy
Cortisol is a laboratory equipment product that measures the concentration of the hormone cortisol in biological samples. Cortisol is a steroid hormone produced by the adrenal gland and plays a crucial role in the body's stress response, metabolism, and immune function. This product is designed to provide accurate and reliable measurements of cortisol levels, which can be used for various clinical and research applications.
Sourced in United States, China, United Kingdom, Germany, Italy, France, Sao Tome and Principe
Corticosterone is a laboratory reagent used in scientific research. It is a naturally occurring steroid hormone produced by the adrenal glands in various species. Corticosterone plays a role in the regulation of metabolism, immune function, and stress response. As a research tool, it is often utilized in studies involving endocrinology, neuroscience, and pharmacology.
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
Sourced in United States, Germany, United Kingdom, Israel, Canada, Austria, Belgium, Poland, Lao People's Democratic Republic, Japan, China, France, Brazil, New Zealand, Switzerland, Sweden, Australia
GraphPad Prism 5 is a data analysis and graphing software. It provides tools for data organization, statistical analysis, and visual representation of results.
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
More about "Cortisone"
Cortisone, also known as Compound E or hydrocortisone, is a powerful glucocorticoid hormone that plays a vital role in regulating metabolism, immune function, and inflammation.
Produced naturally by the adrenal gland, this steroid has been extensively used in medical treatments for a variety of conditions, including asthma, arthritis, and autoimmune disorders.
Cortisone exerts its effects by binding to glucocorticoid receptors, which then modulate the expression of target genes involved in these key physiological processes.
Researchers continue to explore the optimal use of cortisone-based therapies, developing new protocols and products to enhance efficiency and minimize side effects.
Closely related to cortisone are other important glucocorticoids like dexamethasone, cortisol, and corticosterone.
These compounds share similar mechanisms of action and therapeutic applications, though they may differ in potency and specific effects.
To support data-driven research in this area, PubCompare.ai offers a cutting-edge platform that helps identify the best cortisone-related protocols from the latest literature, preprints, and patents.
By analyzing and comparing a wealth of scientific data, the AI-driven system empowers researchers to make informed decisions and drive advancements in this critical field of biomedical science.
Whether you're working with cortisone, dexamethasone, or other related compounds, PubCompare.ai can help you navigate the latest research, optimize your experimental protocols, and enhance the reproducibility and accuracy of your studies.
Experince the future of data-driven research today and unlock the power of cortisone-based therapies.
Produced naturally by the adrenal gland, this steroid has been extensively used in medical treatments for a variety of conditions, including asthma, arthritis, and autoimmune disorders.
Cortisone exerts its effects by binding to glucocorticoid receptors, which then modulate the expression of target genes involved in these key physiological processes.
Researchers continue to explore the optimal use of cortisone-based therapies, developing new protocols and products to enhance efficiency and minimize side effects.
Closely related to cortisone are other important glucocorticoids like dexamethasone, cortisol, and corticosterone.
These compounds share similar mechanisms of action and therapeutic applications, though they may differ in potency and specific effects.
To support data-driven research in this area, PubCompare.ai offers a cutting-edge platform that helps identify the best cortisone-related protocols from the latest literature, preprints, and patents.
By analyzing and comparing a wealth of scientific data, the AI-driven system empowers researchers to make informed decisions and drive advancements in this critical field of biomedical science.
Whether you're working with cortisone, dexamethasone, or other related compounds, PubCompare.ai can help you navigate the latest research, optimize your experimental protocols, and enhance the reproducibility and accuracy of your studies.
Experince the future of data-driven research today and unlock the power of cortisone-based therapies.