Log In Sign up
The largest database of trusted experimental protocols
> Chemicals & Drugs > Organic Chemical > CPT-11

CPT-11

CPT-11 (Irinotecan) is a topoisomerase I inhibitor used in the treatment of various cancers, including colorectal, lung, and ovarian.
It works by interfering with DNA replication and inducing cell death in rapidly dividing cancer cells.
PubCompare.ai's AI-driven tools can help researchers optimize CPT-11 research protocols, identify the most effective products and treatments, and drive their research forward with precision and ease.
Expereince the power of AI-driven insights to advance your CPT-11 studies.

Most cited protocols related to «CPT-11»

1
Folfiri and Folfox Therapy in Mice
Cited 6 times
CD2F1 male mice (n = 8; Harlan, Indianapolis, IN) were administered intraperitoneally (i.p.) Folfiri (5-fluorouracil, leucovorin, CPT-11) or Folfox (5-fluorouracil, leucovorin, oxaliplatin) for up to 5 consecutive weeks. All drugs were purchased from Sigma Aldrich (St. Louis, MO). The dosing schedule is shown in Table S2. Control mice received an equal volume of vehicle. The animals were weighed daily and their food consumption was recorded. At time of sacrifice, several tissues were collected, weighed, snap frozen in liquid nitrogen and stored at −80°C for further studies. The tibialis anterior muscle was frozen in liquid nitrogen-cooled isopentane, mounted in OCT and stored for morphological analyses.
Barreto R., Waning D.L., Gao H., Liu Y., Zimmers T.A, & Bonetto A. (2016). Chemotherapy-related cachexia is associated with mitochondrial depletion and the activation of ERK1/2 and p38 MAPKs. Oncotarget, 7(28), 43442-43460.
Full text: Click here
Publication 2016
Animals CPT-11 Fluorouracil Food Freezing isopentane Leucovorin Males Mice, House Nitrogen Oxaliplatin Pharmaceutical Preparations Tibial Muscle, Anterior Tissues
2
Weekly Alternating FIr-B/FOx Chemotherapy for MCRC
Cited 4 times
This was a single-arm, multicenter phase II study evaluating activity of weekly alternating 5-FU, CPT-11, BEV and OHP (FIr-B/FOx) as first-line treatment of MCRC.
FIr-B/FOx or "Poker" association consisted of 5-FU associated to alternating CPT-11/BEV or l-OHP according to the following weekly schedule: TFI/5-FU (Fluorouracil Teva®, Teva), 900 mg/m2/die, over 12-hour (from 10:00 p.m to 10:00 a.m.), days 1-2, 8-9, 15-16 and 22-23; CPT-11 (Campto®, Pfizer), 160 mg/m2, administered over 90 minutes as an intravenous infusion in 250 ml of NaCl 0.9%, days 1 and 15; BEV (Avastin®, Roche), 5 mg/kg, administered over 90 minutes at the first, 60 minutes at the second and 30 minutes from the third time, intravenous infusion in 100 ml of NaCl 0.9%, days 1 and 15; l-OHP (Eloxatin®, Sanofi-Aventis) over 2-hours as an intravenous infusion in 250 ml of dextrose 5%, at the dose of 60-70-80 mg/m2, days 8 and 22. Cycles repeated every 4 weeks. 5-FU was administered by a portable pump (CADD Plus, SEVIT) using a venous access device.
Bruera G., Santomaggio A., Cannita K., Baldi P.L., Tudini M., De Galitiis F., Mancini M., Marchetti P., Antonucci A., Ficorella C, & Ricevuto E. (2010). "Poker" association of weekly alternating 5-fluorouracil, irinotecan, bevacizumab and oxaliplatin (FIr-B/FOx) in first line treatment of metastatic colorectal cancer: a phase II study. BMC Cancer, 10, 567.
Full text: Click here
Publication 2010
Avastin CPT-11 Eloxatin Glucose Intravenous Infusion Medical Devices Normal Saline Oxaliplatin Veins
3
Prostate Cancer Cell Lines Protocol
Cited 3 times
RWPE-1,LNCaP, DU145,PC-3and 22RV1 cells were purchased from ATCC (Manassas, VA)in February 2002 and maintained in a humidified atmosphere of 5% CO2, RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and antibiotics except that RWPE-1 cells were maintained in Keratinocyte-SFM media (Invitrogen, Carlsbad, CA). LAPC-4 was obtained from UCLA and C4-2 was purchased from UroCor (Oklahoma City, OK) in May 2002 as described in our previous publications (21 (link), 22 (link)). BPH1 cell line was kindly provided by Dr Long-cheng Li (Department of Urololgy, University of California at San Francisco) in August 2011. These cell lines were authenticated using PromegaPowerPlex® technology and carried out at Genetica® DNA Laboratories (Burlington, NC) in January 2014. Alternol (99.9% purity) was a kind gift from Strand Biotech Co (Shantou, China) and its structural scheme is shown in Fig 1A. It was dissolved in dimethyl sulfoxide (DMSO) as a 10 mM stock solution. Antibodies for caspase-3, PARP, Bax, Bif-1, Bcl-2, Bcl-xL and BAD were obtained from Cell Signal (Danvers, MA). Pre-validated small interfering RNAs, Actin antibody, CPT-11, n-acetylcysteine (N-Ac), dihydrolipoic acid (DHLA), MG132, E-64, PD150606 and Bax channel blocker were purchased from Santa Cruz Biotech (Santa Cruz, CA). All the reagents were prepared according to the manufacturer's instruction and used as described in the figure legends. Final concentrations of the solvent did not exceed 0.1% of the culture media. Bax expression construct pCEP4-HA-hBax (23 (link)) was obtained from Add gene (Cambridge, MA) and the control empty vector was purchased from Invitrogen (Carlsbad, CA). DNA Ladder Detection Kit and Caspase-9 colorimetric activity assay kit were purchased from Millipore (Billerica, MA). Preassembled assay kit for total ROS detection was purchased from Enzo Life Sciences (Farmingdale, NY). Annexin V-FITC Apoptosis Detection kit was purchased from BD PharMingen (San Diego, CA). Mitochondrial membrane potential assay kit with the fluorescent dye 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolyl carbocyanine iodide (JC-1), Lipofectamine 2000 and RNAiMAX were purchased from Invitrogen (Carlsbad, CA). DAKO LSAB+ System kit was purchased from DAKO USA (Carpinteria, CA).
Tang Y., Chen R., Huang Y., Li G., Huang Y., Chen J., Duan L., Zhu B.T., Thrasher J.B., Zhang X, & Li B. (2014). Natural compound Alternol induces oxidative stress-dependent apoptotic cell death preferentially in prostate cancer cells. Molecular cancer therapeutics, 13(6), 1526-1536.
Publication 2014
Acetylcysteine Actins Alternol Antibiotics Antibodies Apoptosis Atmosphere BCL2 protein, human Biological Assay Carbocyanines Caspase 3 Caspase 9 Cell Lines Cells Colorimetry CPT-11 Culture Media dihydrolipoic acid Fetal Bovine Serum FITC-annexin A5 Fluorescent Dyes Genes Immunoglobulins Iodides Keratinocyte lipofectamine 2000 Membrane Potential, Mitochondrial MG 132 PD 150606 RNA, Small Interfering Signal Transduction Solvents Sulfoxide, Dimethyl
4
Pharmacokinetics of CPT-11 and IT-101
Cited 3 times
Localized s.c. human xenograft-bearing animals with tumor volumes (TVs) reaching approximately 500–800 mm3 were randomly divided into two treatment groups of 20 animals each; group 1: CPT-11 (100 mg/kg, intraperitoneal [i.p.], single dose) and group 2: IT-101 (10 mg/kg, i.v., single dose). Then tumor specimens and plasma from five mice in each treatment group were corrected at four time points (before dosing, 2, 24, and 48 hours after dosing) to measure plasma and tumor concentrations of the compounds and their active metabolites. Measurements of plasma and tumor concentrations of IT-101 and CPT were carried out for SCID mice treated with IT-101, whereas those of CPT-11 and SN-38 were carried out for mice treated with CPT-11. The method of these measurements has been previously described (26 (link)).
Numbenjapon T., Wang J., Colcher D., Schluep T., Davis M.E., Duringer J., Kretzner L., Yen Y., Forman S.J, & Raubitschek A. (2009). Preclinical Results of Camptothecin-Polymer Conjugate (IT-101) in Multiple Human Lymphoma Xenograft Models. Clinical cancer research : an official journal of the American Association for Cancer Research, 15(13), 4365-4373.
Publication 2009
Animals CPT-11 Heterografts Homo sapiens IT-101 Mus Neoplasms Plasma SCID Mice SN 38
5
Immunoblotting for ER Stress Response
Cited 2 times
MIP/ZEO, MIP/SP, or HCT116 cells were seeded in 60 -mm dishes. After 48 h, cells were treated with 5-FU, CPT-11 or tunicamycin at indicated concentration and time periods. Cells were extracted in lysis buffer [1% Triton-X 100, 120 mM NaCl, 50 mM Tris-HCl, pH 7.5] supplemented with 1% proteinase inhibitor cocktail (Sigma) followed by protein quantification with Bradford assay (Bio-Rad). Equal amount of proteins were separated by SDS-PAGE under reducing conditions and electrotransferred onto a PVDF membrane (Millipore). Blots were incubated with primary antibodies in TBST (TBS containing 0.1% Tween-20) overnight at 4 °C. [1:1000 anti-GRP78 (sc-13968, Santa Cruz and cat. 3177 Cell Signaling Technology (CST)); 1:1000 anti-SPARC (AON-5031, Haematologic Technologies Inc.); 1:200 anti-pPERK (cat. 649402, BioLegend); 1:1000 anti-PERK (CST); 1:1000 anti-peIF2α (cat. 3597, CST); 1:1000 anti-eIF2α (sc-11386, Santa Cruz); 1:250 anti-ATF4 (sc-200, Santa Cruz); 1:1000 anti-pIRE1α (ab124945, Abcam); 1:1000 anti-IRE1α (cat. 3294, CST); 1:10,000 anti-β-actin (G043, ABM); 1:1000 anti-calnexin (cat. 2679, CST) and 1:2000 anti-DNA-PK (cat. 4602, CST)]. The blots were then incubated with anti-mouse immunoglobulin (IgG)-HRP (SH023, ABM) or anti-rabbit IgG-HRP (cat. 7074, CST) followed by enhanced chemiluminescence (ECL) detection. For immunoprecipitation analysis, conformation-specific secondary antibodies were used [1: 5000 anti-mouse IgG-HRP (Rockland) and 1:5000 anti-rabbit IgG-HRP (Rockland)].
Chern Y.J., Wong J.C., Cheng G.S., Yu A., Yin Y., Schaeffer D.F., Kennecke H.F., Morin G, & Tai I.T. (2019). The interaction between SPARC and GRP78 interferes with ER stress signaling and potentiates apoptosis via PERK/eIF2α and IRE1α/XBP-1 in colorectal cancer. Cell Death & Disease, 10(7), 504.
Full text: Click here
Publication 2019
Actins anti-IgG Antibodies Antibodies, Anti-Idiotypic ATF4 protein, human Biological Assay Buffers Calnexin Cells Chemiluminescence CPT-11 ERN1 protein, human Glucose Regulated Protein 78 kDa HCT116 Cells Hyperostosis, Diffuse Idiopathic Skeletal Immunoprecipitation Mus polyvinylidene fluoride Protease Inhibitors Protein Kinase, DNA-Dependent Proteins Rabbits SDS-PAGE Sodium Chloride SPARC protein, human Tissue, Membrane Triton X-100 Tromethamine Tunicamycin Tween 20

Most recents protocols related to «CPT-11»

1
Tissue Distribution of CPT-11 Formulations
KM mice were randomly divided into a blank group and an administration group (the CPT-11, CPT-11-Lip, and CA-CPT-11-Lip groups). In the administration group, CPT-11, CPT-11-Lip, and CA-CPT-11-Lip were administered to mice by gavage at a dose of 50 mg/Kg [47 (link)], respectively. Animals were sacrificed at 0.5, 1, 2, 3, 4, 8, and 12 h after administration and the heart, liver, spleen, lungs, and kidneys were gathered. The tissue samples were weighed and added with 2 times the saline; the samples were homogenized with a homogenizer (Tissuelyser-48, Shanghai Jingxin Technology, Shanghai, China). Then, the tissue solution was centrifuged at 4 °C and 10,000 r/min for 10 min, the supernatant was collected, an equal amount of acetonitrile was added to completely precipitate the protein, and the supernatant was collected by centrifugation again and stored at −80 °C. The CPT-11 content was detected at 370 nm using a reversed-phase column (Zhongpu Science, 4.6 × 250 mm, 5 μm). The mobile phase was a mixture of acetonitrile-0.1% trifluoroacetic acid aqueous solution (from 0 to 15 min, the volume of acetonitrile was increased from 20% to 80%) at a flow rate of 1 mL/min and an injection volume of 20 μL. Standard tissue samples were prepared from blank tissue samples and CPT-11 standard solution, and a standard curve was established to calculate the concentration of CPT-11 in each tissue.
Zhou T., Liu Y., Lei K., Liu J., Hu M., Guo L., Guo Y, & Ye Q. (2023). A “Trojan Horse” Strategy: The Preparation of Bile Acid-Modifying Irinotecan Hydrochloride Nanoliposomes for Liver-Targeted Anticancer Drug Delivery System Study. Molecules, 28(4), 1577.
Full text: Click here
Publication 2023
acetonitrile Animals Centrifugation CPT-11 Heart Kidney Kunming mice Liver Lung Mice, House Proteins Saline Solution Spleen Tissues Trifluoroacetic Acid Tube Feeding
2
Optimized Liposomal Irinotecan Formulation
According to the BBD results, CPT-11-Lip and CA-CPT-11-Lip (n = 3) were prepared under the optimal process. Free-formed drug and liposomes were separated using a Sephadex G-50 gel chromatography method [29 (link)]. The inner diameter of the chromatographic column was 2.5 cm. The loading height of the dextran gel G-50 was about 15 cm. The sample volume of liposome was 1 mL, and pure water was used as the elution solvent with a flow rate of 0.5 mL/min. Formula (1) and (2) were used to calculate the EE and DL of liposomes, respectively.
EE%= encapsulated drug contenttotal drug content×100%
DL%=encapsulated drug contentweight of carrier×100%
Zhou T., Liu Y., Lei K., Liu J., Hu M., Guo L., Guo Y, & Ye Q. (2023). A “Trojan Horse” Strategy: The Preparation of Bile Acid-Modifying Irinotecan Hydrochloride Nanoliposomes for Liver-Targeted Anticancer Drug Delivery System Study. Molecules, 28(4), 1577.
Full text: Click here
Publication 2023
Chromatography CPT-11 Dextran Drug Carriers Drugs, Non-Prescription Gel Chromatography Liposomes Pharmaceutical Preparations sephadex G 50 Solvents
3
In Vitro Toxicity of Liposomal Formulations on HepG-2 Cells
The in vitro toxicity of liposomes to hepatoma cells (HepG-2) was evaluated by the CCK-8 method. The log-phase HepG-2 cells with good growth status were seeded in 96-well plates at 4 × 103 cells per well. After incubation for 24 h, appropriate concentrations of CPT-11, CPT-11-Lip, and CA-CPT-11-Lip were added, respectively. Sampled were then incubated for another 48 h, then we discarded the culture medium, added 10 μL/well of CCK-8 solution in the dark, and continued to incubate for 1.5 h. Finally, using a Multifunctional Enzyme Analyzer (SpectraMax iD, Thermo Scientific, Waltham, MA, USA), we detected the absorbance at the wavelength of 450 nm. The cell viability was calculated according to Formula (4): Cell viability %=OD experimental groupOD blank group×100%
Zhou T., Liu Y., Lei K., Liu J., Hu M., Guo L., Guo Y, & Ye Q. (2023). A “Trojan Horse” Strategy: The Preparation of Bile Acid-Modifying Irinotecan Hydrochloride Nanoliposomes for Liver-Targeted Anticancer Drug Delivery System Study. Molecules, 28(4), 1577.
Full text: Click here
Publication 2023
Cells Cell Survival CPT-11 Culture Media Hepatocellular Carcinomas Liposomes Multifunctional Enzymes Sincalide
4
Liposomal Delivery of CPT-11 and CA
Liposomes were prepared by film dispersion method. First, 100 mg of lecithin and 20 mg of cholesterol were weighed into a 250 mL eggplant-shaped bottle, and 20 mL of a chloroform–methanol (9:1) mixed solution was added for sonication and placed on a rotary evaporator (RE-52AA, ShangHai Yarong Biochemistry Instrument Factory, Shanghai, China) at 40 °C to remove the organic solvent under vacuum until a uniform film formed on the bottle wall. Then, 10 mL of 1.0 mg/mL CPT-11 aqueous solution was added to the lipid film and hydrated for 1.5 h. The formed liposomes’ solution (CPT-11-Lip) was then sonicated for 5 min in an ice-water bath using an ultrasonicator (KM-500DE, Kunshan Meimei Ultrasonic Instrument Co., Ltd., Kunshan, China) to disperse it uniformly, and then filtered through 0.45 and 0.22 μm microporous membranes sequentially and stored at 4 °C. CA-CPT-11-Lip was prepared in the same manner with an additive of 15 mg of CA in the 250 mL eggplant-shaped bottle.
Zhou T., Liu Y., Lei K., Liu J., Hu M., Guo L., Guo Y, & Ye Q. (2023). A “Trojan Horse” Strategy: The Preparation of Bile Acid-Modifying Irinotecan Hydrochloride Nanoliposomes for Liver-Targeted Anticancer Drug Delivery System Study. Molecules, 28(4), 1577.
Full text: Click here
Publication 2023
Aubergine Bath Chloroform Cholesterol CPT-11 Lecithin Lipids Liposomes Methanol Solvents Tissue, Membrane Ultrasonics Vacuum
5
Irinotecan-Based Lipid Nanoparticle Formulation
Irinotecan hydrochloride (CPT-11, purity > 99%, Chengdu Refensi Biotechnology Co., Ltd., Chengdu, China), cholic acid (purity ≥ 98%, Chengdu McLean Biotechnology Co., Ltd., Chengdu, China), lecithin (purity ≥ 99%, Chengdu Kelon Chemical Co., Ltd., Chengdu, China), and cholesterol (purity > 95%, Chengdu Kelon Chemical Co., Ltd., Chengdu, China) were obtained. Acetonitrile was HPLC grade. All other reagents were analytical grade, and purified deionized water was used throughout.
HepG2 cell lines were provided by the State Key Laboratory of Southwestern Chinese Medicine Resources. Cells were cultured in DMEM medium containing 10% fetal bovine serum and 1% penicillin-streptomycin at 37 °C in a 5% CO2 constant temperature incubator.
KM mice (18–22 g) were purchased from Spearfish (Beijing) Biotechnology Co., Ltd. (Beijing, China). All mice were acclimatized to their new environment for one week, during which they were kept in light for 12 h and dark for 12 h per day, with appropriate control of diet and free access to water. Mice were prohibited from ingesting food for 12 h before the experiment, but drinking water was not restricted. All animal experimental protocols were approved by the Animal Research Ethics Committee of Chengdu University of Traditional Chinese Medicine (NO.2022-29).
Zhou T., Liu Y., Lei K., Liu J., Hu M., Guo L., Guo Y, & Ye Q. (2023). A “Trojan Horse” Strategy: The Preparation of Bile Acid-Modifying Irinotecan Hydrochloride Nanoliposomes for Liver-Targeted Anticancer Drug Delivery System Study. Molecules, 28(4), 1577.
Full text: Click here
Publication 2023
acetonitrile Animal Ethics Committees Animals Cells Chinese Cholesterol Cholic Acid CPT-11 Culture Media Diet Ethics Committees, Research Fetal Bovine Serum Food Hep G2 Cells High-Performance Liquid Chromatographies Irinotecan Hydrochloride Kunming mice Lecithin Light Mus Penicillins Streptomycin

Top products related to «CPT-11»

1
Cpt 11 by Merck Group
Sourced in United States
CPT-11 is a laboratory equipment product manufactured by Merck Group. It is designed for use in various research and analytical applications. The core function of CPT-11 is to provide a reliable and efficient tool for researchers and scientists working in the field of chemistry, biology, or related disciplines. Further details on the intended use or specific features of this product are not available.
2
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
3
Dimethyl sulfoxide (dmso) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
4
7 ethyl 10 hydroxycamptothecin sn 38 by Merck Group
Sourced in United States, Macao, Sao Tome and Principe, Italy
7-ethyl-10-hydroxycamptothecin (SN-38) is a chemical compound that serves as an active metabolite of the antitumor agent irinotecan. It functions as a topoisomerase I inhibitor, which is a key mechanism in its anticancer activity.
5
Milli q system by Merck Group
Sourced in United States, Germany, France, United Kingdom, Italy, Morocco, Spain, Japan, Brazil, Australia, China, Belgium, Ireland, Denmark, Sweden, Canada, Hungary, Greece, India, Portugal, Switzerland
The Milli-Q system is a water purification system designed to produce high-quality ultrapure water. It utilizes a multi-stage filtration process to remove impurities, ions, and organic matter from the input water, resulting in water that meets the strict standards required for various laboratory applications.
6
Hct116 by American Type Culture Collection
Sourced in United States, China, Germany, United Kingdom, Japan, Australia, France, Israel, Spain, Poland, Italy
The HCT116 cell line is a human colorectal carcinoma cell line that is widely used in research. It is a commonly used model system for studying various aspects of cancer biology and drug development.
7
Irinotecan cpt 11 by Merck Group
Sourced in United States
Irinotecan (CPT-11) is a synthetic analog of the natural alkaloid camptothecin, used as an active pharmaceutical ingredient in various cancer treatment medications. It functions as a topoisomerase I inhibitor, which interferes with the process of DNA replication in rapidly dividing cancer cells.
8
Cpt 11 by Pfizer
Sourced in Australia
CPT-11 is a laboratory product used for research purposes. It is a chemical compound that functions as a topoisomerase I inhibitor. The core function of CPT-11 is to inhibit the activity of topoisomerase I, an enzyme involved in the regulation of DNA topology.
9
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
10
N methyl n trifluoroacetamide by Merck Group
Sourced in United States
N-methyl-N-trifluoroacetamide is a chemical compound used as a derivatizing agent in analytical chemistry. It is commonly used to derivatize and analyze organic compounds, particularly alcohols and amines, using techniques such as gas chromatography and mass spectrometry.

More about "CPT-11"

Irinotecan, also known as CPT-11, is a potent topoisomerase I inhibitor that has been widely used in the treatment of various types of cancer, including colorectal, lung, and ovarian.
This chemotherapeutic agent works by interfering with the DNA replication process, leading to the induction of cell death in rapidly dividing cancer cells.
To conduct effective research on CPT-11, researchers often utilize a range of related compounds and reagents, such as FBS (Fetal Bovine Serum) and DMSO (Dimethyl Sulfoxide), which are commonly used in cell culture experiments.
Additionally, the active metabolite of CPT-11, 7-ethyl-10-hydroxycamptothecin (SN-38), is an important factor to consider in CPT-11 research, as it plays a crucial role in the drug's mechanism of action.
Researchers may also employ advanced techniques, like the Milli-Q system, to ensure the highest quality of water used in their experiments.
Moreover, the use of cell lines, such as HCT116, can provide valuable insights into the effects of CPT-11 on cancer cells.
To optimize research protocols and identify the most effective products and treatments, researchers can leverage the power of AI-driven tools, like those offered by PubCompare.ai.
These tools can help researchers navigate the vast landscape of scientific literature, pre-prints, and patents, enabling them to make informed decisions and drive their CPT-11 studies forward with precision and ease.
Ultimately, the combination of CPT-11, related compounds, advanced techniques, and AI-driven insights can empower researchers to unlock new discoveries and advancements in the field of cancer treatment and management.