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Decane

Decane is a straight-chain alkane with the molecular formula C10H22.
It is a clear, colorless liquid with a mild odor.
Decane is used as a solvent, fuel, and in the production of other chemicals.
It can be found naturally in petroleum and is a component of some plant and animal fats.
Decane has a boiling point of 174°C and is considered a moderately flammable substance.
It is insoluble in water but miscible with many organic solvents.
Decane plays a role in various industrial and research applications, making it an important compound to understand and utilize efectivley.

Most cited protocols related to «Decane»

1
Planar Bilayer Integration of Engineered Connector
Cited 18 times
A two-step approach was used to incorporate the connector into the planar bilayer lipid membrane (BLM). The first step was the preparation of unilamellar lipid vesicles containing the reengineered connector as described above. The next step was to fuse the extruded liposome into a planar BLM (Fig. 2i). The fluidity of the lipid bilayer was demonstrated by FRAP (Fluorescence Recovery After Photobleaching) (Fig. 2h). An excitation light was focused continuously on the bilayer to bleach the dye. The photobleached area appeared dark. But after the light was off, it gradually recovered due to the diffusion of the fluorescent lipid.
A standard BLM chamber (BCH-1A from Eastern Sci LLC) was utilized to form horizontal BLMs. A thin Teflon film with an aperture of 70–120 µm (TP-01 from Easter Sci LLC) or 180–250 µm (TP-02 from Easter Sci LLC) in diameter was used as a partition to separate the chamber into cis- (working volume 250 µL) and trans- (working volume 2.5 mL) compartments. After the aperture was pre-painted with 0.5 µL 3% (w/v) DPhPC n-decane solution twice to ensure the complete coating of the entire edge of the aperture, these compartments were filled with conducting buffers (5 mM Tris/pH 7.9, TMS, or 5 mM HEPES/pH 7.9, with varying concentration of NaCl or KCl).
Formation of the bilayer membrane on the partition is a key step for connector insertion into the bilayer (Fig. 2i). Considering all experiments, the occurrence of successful connector insertions was about 47–83%, which varied from person to person based on BLM experience and the quality of prepared proteoliposomes. So far, we have carried out a total of 280 separate BLM experiments in which successful connector insertions were found.
For single conductance measurements, the giant liposome/connector complex prepared earlier must be extruded using a polycarbonate membrane with pore size of 200 nm or 400 nm to generate small unilamellar liposomes. This liposome stock solution was further diluted by 10–20 fold for the BLM experiments before use. For insertion of connectors, 0.5–2 µL of the diluted liposome solution was loaded into the cis-chamber.
Conductance was measured in two ways: the first was derived at specific but constant holding potentials, and the second from the slope of the current trace induced by a scanning potential starting at −100 mV and ramping to 100 mV after incorporation of GP10 connector into the lipid membrane (Fig. 3f and 3g).
Wendell D., Jing P., Geng J., Subramaniam V., Lee T.J., Montemagno C, & Guo P. (2009). Translocation of double stranded DNA through membrane adapted phi29 motor protein nanopore. Nature nanotechnology, 4(11), 765-772.
Publication 2009
BLM protein, human Buffers decane Diffusion Gigantism HEPES Light Lipid Bilayers Lipids Liposomes Membrane Fluidity polycarbonate proteoliposomes Sodium Chloride Teflon Tissue, Membrane Tromethamine Unilamellar Liposomes Unilamellar Vesicles
2
Comprehensive GC-MS and LC-MS Metabolomics Protocol
Cited 17 times
For GC–MS analysis a protocol according to Weckwerth et al. was used (Weckwerth et al. 2004 (link)). Deep frozen plant material was ground to a fine powder using a mortar and pestle under constant adding of liquid nitrogen. About 45 mg of each replicate was transferred to pre-cooled reaction tubes. For the extraction process, 1 ml of ice cold extraction mixture (methanol:chloroform:water, 5:2:1, v:v:v) was subsequently added. Additionally, 10 μl of internal 13C6-Sorbitol standard were added into each tube. Tubes were vortexed for several seconds and incubated on ice for 8 min to achieve a good extraction. Hereupon, the samples were centrifuged for 4 min at 14,000×g, separating the soluble compounds from remaining cell structure components. For phase separation, the supernatant was then carried over into a new tube containing 500 μl deionized water and 200 μl chloroform. After 2 min of centrifugation at 14,000×g, the water/methanol phase, containing the polar metabolites, was separated from the subjacent chloroform phase and completely dried out overnight.
Samples were derivatised by dissolving the dried pellet in 20 μl of a 40 mg methoxyamine hydrochloride per 1 ml pyridine solution and incubation on a thermoshaker at 30 °C for 90 min. After adding of 80 μL of N-methyl-N-trimethylsilyltrifluoroacetamid (MSTFA), the mixture was again incubated at 37 °C for 30 min with strong shaking.
A solution of even-numbered alkanes (Decane C10, Dodecane C12, Tetradecane C14, Hexadecane C16, Octadecane C18, Eicosane C20, Docosane C22, Tetracosane C24, Hexacosane C26, Octacosane C28, Triacontane C30, Dotriacontane C32, Tetratriacontane C34, Hexatriacontane C36, Octatriacontane C38, Tetracontane C40) was spiked into the derivatized sample before GC–MS analysis in order to infer the retention time and create the retention index.
For LC–MS analysis, frozen plant leaf material was ground as for GC–MS sample preparation, followed by addition of 1 ml pre-chilled 80/20 v:v MeOH/H2O extraction solution containing each 1 μg of the internal standards Ampicillin and Chloramphenicol per 50 mg of fresh weight. Samples were hereupon centrifuged at 15,000×g for 15 min and the supernatant was placed into a new tube and completely dried out overnight. The resulting pellet was then dissolved in 100 μl of a 50/50 v:v MeOH/H2O solution and centrifuged again for 15 min at 20,000×g. The remaining supernatant was then filtered through a STAGE tip (Empore/Disk C18, diameter 47 mm) into a vial with a micro insert tip. Before analysis lipid components were removed by adding 500 µl of chloroform, centrifugation and separation of the non-polar-phase to avoid contamination of the ESI ion transfer capillary.
Doerfler H., Lyon D., Nägele T., Sun X., Fragner L., Hadacek F., Egelhofer V, & Weckwerth W. (2012). Granger causality in integrated GC–MS and LC–MS metabolomics data reveals the interface of primary and secondary metabolism. Metabolomics, 9(3), 564-574.
Publication 2012
Alkanes Ampicillin Capillaries Cellular Structures Centrifugation Chloramphenicol Chloroform Cold Temperature decane DNA Replication docosane dotriacontane eicosane Empore Freezing Gas Chromatography-Mass Spectrometry hexadecane Lipids Methanol methoxyamine methoxyamine hydrochloride n-dodecane Neoplasm Metastasis Nitrogen octacosane octadecane octatriacontane PER1 protein, human Plant Leaves Plants Powder pyridine pyridine hydrochloride Retention (Psychology) Sorbitol Strains tetracosane tetradecane
3
Planar Bilayer Integration of Engineered Connector
Cited 14 times
A two-step approach was used to incorporate the connector into the planar bilayer lipid membrane (BLM). The first step was the preparation of unilamellar lipid vesicles containing the reengineered connector as described above. The next step was to fuse the extruded liposome into a planar BLM (Fig. 2i). The fluidity of the lipid bilayer was demonstrated by FRAP (Fluorescence Recovery After Photobleaching) (Fig. 2h). An excitation light was focused continuously on the bilayer to bleach the dye. The photobleached area appeared dark. But after the light was off, it gradually recovered due to the diffusion of the fluorescent lipid.
A standard BLM chamber (BCH-1A from Eastern Sci LLC) was utilized to form horizontal BLMs. A thin Teflon film with an aperture of 70–120 µm (TP-01 from Easter Sci LLC) or 180–250 µm (TP-02 from Easter Sci LLC) in diameter was used as a partition to separate the chamber into cis- (working volume 250 µL) and trans- (working volume 2.5 mL) compartments. After the aperture was pre-painted with 0.5 µL 3% (w/v) DPhPC n-decane solution twice to ensure the complete coating of the entire edge of the aperture, these compartments were filled with conducting buffers (5 mM Tris/pH 7.9, TMS, or 5 mM HEPES/pH 7.9, with varying concentration of NaCl or KCl).
Formation of the bilayer membrane on the partition is a key step for connector insertion into the bilayer (Fig. 2i). Considering all experiments, the occurrence of successful connector insertions was about 47–83%, which varied from person to person based on BLM experience and the quality of prepared proteoliposomes. So far, we have carried out a total of 280 separate BLM experiments in which successful connector insertions were found.
For single conductance measurements, the giant liposome/connector complex prepared earlier must be extruded using a polycarbonate membrane with pore size of 200 nm or 400 nm to generate small unilamellar liposomes. This liposome stock solution was further diluted by 10–20 fold for the BLM experiments before use. For insertion of connectors, 0.5–2 µL of the diluted liposome solution was loaded into the cis-chamber.
Conductance was measured in two ways: the first was derived at specific but constant holding potentials, and the second from the slope of the current trace induced by a scanning potential starting at −100 mV and ramping to 100 mV after incorporation of GP10 connector into the lipid membrane (Fig. 3f and 3g).
Wendell D., Jing P., Geng J., Subramaniam V., Lee T.J., Montemagno C, & Guo P. (2009). Translocation of double stranded DNA through membrane adapted phi29 motor protein nanopore. Nature nanotechnology, 4(11), 765-772.
Publication 2009
BLM protein, human Buffers decane Diffusion Gigantism HEPES Light Lipid Bilayers Lipids Liposomes Membrane Fluidity polycarbonate proteoliposomes Sodium Chloride Teflon Tissue, Membrane Tromethamine Unilamellar Liposomes Unilamellar Vesicles
4
PFAS Transport Behavior in Porous Media
Cited 9 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2018
5
Ion channel reconstitution in lipid bilayers
Cited 8 times
Liposomes containing CLC-ec1 at high protein density were incorporated under salt-gradient conditions into bilayers formed from POPE/POPG (7.5:2.5 mg/ml) in n-decane, using a horizontal planar lipid bilayer system (Chen and Miller, 1996 (link); Nimigean and Miller, 2002 (link)). Briefly, two aqueous chambers (0.3–0.7 ml) are separated by a partition with a ∼50-μm hole where the bilayer is formed (35–100 pF). 1 μL of CLC-ec1–containing liposomes was added to the upper chamber to a preformed bilayer, and sometimes to favor vesicle fusion ∼1 μl of 1.5–3 M KCl was added right after the vesicles. The standard high-salt (HS) solution was: 290 mM KCl, 10 mM HCl, 5 mM histidine, 5 mM glutamic acid, pH 3 or 7 with KOH. The low-salt solution (LS) was: 35 mM KCl and 10 mM HCl containing the same buffers and adjusted to pH 3 or 7 with KOH. The low-Cl (LCL) solution was: 5 mM HCl, 285 mM K2SO4, 10 mM H2SO4, 5 mM histidine, 5 mM glutamate, pH 3 or 7 with KOH. For selectivity experiments 255 mM KCl in the HS solution was replaced with an equivalent amount of the potassium salt of the desired anion. All selectivity experiments were performed at symmetrical pH 3.
Currents were recorded using the Clampex 8.2 program (Axon Instruments, Inc.) with an Axopatch 200B amplifier (Axon Instruments, Inc.) sampled at 100 μs and filtered at 1 kHz. Data were analyzed using Clampfit (Axon Instruments, Inc.), Ana (written by Dr. Michael Pusch), and SigmaPlot 8.02. In all cases, voltages were corrected for liquid junction potentials (in most case <1 mV, and in no case >10 mV) and error bars represent the SEM of at least three separate experiments, each in a separate bilayer.
Accardi A., Kolmakova-Partensky L., Williams C, & Miller C. (2004). Ionic Currents Mediated by a Prokaryotic Homologue of CLC Cl− Channels. The Journal of General Physiology, 123(2), 109-119.
Publication 2004
1-palmitoyl-2-oleoylphosphatidylethanolamine Anions Axon Buffers decane Genetic Selection Glutamates Glutamic Acid Histidine Lipid Bilayers Liposomes Potassium Proteins Sodium Chloride

Most recents protocols related to «Decane»

1
Synthesis of Decane-1,10-diyl Bis(Methanesulfonate)
In a 100-mL three-necked round-bottomed
flask equipped
with a magnetic stirring bar, a rubber septum and an argon balloon,
decane-1,10-diol (10.2 g, 58.6 mmol) in pyridine (42 mL), and Ms2O (25.0 g, 144 mol) were added, respectively.16 (link) After stirring for 13.5 h at 0 °C, ice water (50 mL)
was added to quench the reaction and filtered with the Büchner
funnel washing with ice water (30 mL) and cooled hexane (30 mL) to
give a crude product, which was recrystallized from ethyl acetate/methanol
(1:1) to give the titled compound 2c′ (7.42 g, 38%).
Mizota I., Yamamoto T., Kaliyamoorthy S., Shimizu M., Rathinam B., Liu B.T., Kuroki N., Kiyosawa J, & Tanaka H. (2024). Synthesis of Dicarboxylic Acids Comprising an Ether Linkage and Cyclic Skeleton and Its Further Application for High-Performance Aluminum Electrolyte Capacitors. ACS Omega, 9(9), 10628-10639.
Publication 2024
2
Synthesis of Decane-1,10-Diol Tosylate
In a 300-mL two-necked round-bottomed
flask equipped with a magnetic stirring bar, a rubber septum and an
argon balloon, decane-1,10-diol (6.97 g, 40 mmol) in pyridine (27
mL) and TsCl (18.7 g, 98.0 mmol) were added, respectively. After stirring
for 1 h at 0 °C, ice water (50 mL) was added to quench the reaction
and filtered with the Büchner funnel washing with ice water
(50 mL) and cooled hexane (50 mL) to give a crude product, which was
recrystallized from ethyl acetate/hexane (3:1) to give the titled
compound 2c (11.7 g, 61%).
Mizota I., Yamamoto T., Kaliyamoorthy S., Shimizu M., Rathinam B., Liu B.T., Kuroki N., Kiyosawa J, & Tanaka H. (2024). Synthesis of Dicarboxylic Acids Comprising an Ether Linkage and Cyclic Skeleton and Its Further Application for High-Performance Aluminum Electrolyte Capacitors. ACS Omega, 9(9), 10628-10639.
Publication 2024
3
Quantifying Cell Production in NJF-7
Not available on PMC !
NJF-7 was grown in 60 mL glass vials containing 10 mL of medium with decane and FD (5 mM), respectively. Six replicates were conducted for a total of twelve vials. After 6 days of shock incubation, half of the samples were subjected to whole-sample extraction to quantify the residual substrate. The remaining half of the samples were used to determine cell production via the Bradford protein assay, as described in Xie et al. (2020) (link).
Yan M., Gao Z., Xiang X., Wang Q., Song X., Wu Y., Löffler F.E., Zeng J, & Lin X. (2024). Defluorination of monofluorinated alkane by Rhodococcus sp. NJF-7 isolated from soil. AMB Express, 14(1).
Publication 2024
4
Synthesis of Alkylated Ester Compound
In a 100-mL two-necked round-bottomed flask
equipped
with a magnetic stirring bar, a rubber septum and an argon balloon,
NaH (60% dispersion in mineral oil, 2.27 g, 56.8 mmol), and THF (12
mL) were added, respectively. The reaction mixture was cooled to 0
°C and 18-crown-6 (2.99 g, 11.3 mmol) in THF (5.0 mL) and ethyl
2-hydroxypropanoate (5.9 mL, 51.5 mmol) in THF (12 mL) were added
to this reaction mixture. The mixture was stirred for 1 h at 0 °C
then decane-1,10-diyl dimethanesulfonate 2c′ (5.68 g, 17.2 mmol) within THF (21 mL) was added to the reaction
mixture. The reaction mixture was warmed to reflux and stirred for
14 h. The pH was adjusted to 5–6 with 2 M H2SO4 solution to quench the reaction. The whole mixture was extracted
with diethyl ether (3 × 30 mL). The combined organic phases were
washed with H2O (3 × 30 mL), dried (with Na2SO4), and concentrated in vacuo to give a crude product.
The crude product was purified by flash column chromatography on silica
gel (n-hexane/ethyl acetate = 6:1) to give the title
compound 3c (2.17 g, 34%).
Mizota I., Yamamoto T., Kaliyamoorthy S., Shimizu M., Rathinam B., Liu B.T., Kuroki N., Kiyosawa J, & Tanaka H. (2024). Synthesis of Dicarboxylic Acids Comprising an Ether Linkage and Cyclic Skeleton and Its Further Application for High-Performance Aluminum Electrolyte Capacitors. ACS Omega, 9(9), 10628-10639.
Publication 2024
5
Synthesis of Decyl Dialkyl Ester Derivative
In a 50-mL two-necked round-bottomed flask
equipped
with a magnetic stirring bar, a rubber septum and an argon balloon,
NaH (60% dispersion in mineral oil, 2.33 g, 58.3 mmol) and THF (23
mL) were added, respectively. The reaction mixture was cooled to 0
°C and 18-crown-6 (2.41 g, 9.12 mmol) in THF (11 mL) and ethyl
2-hydroxypropanoate (8.0 mL, 69.9 mmol) in THF (19 mL) were added
to this reaction mixture. The mixture was stirred for 1 h at 0 °C
then decane-1,10-diyl bis(4-methylbenzenesulfonate) 2c (11.3 g, 23.3 mmol) within THF (11 mL) was added to the reaction
mixture. The reaction mixture was warmed to reflux and stirred for
21 h. The pH was adjusted to 7 with 5 M H2SO4 solution to quench the reaction. The whole mixture was extracted
with diethyl ether (5 × 10 mL). The combined organic phases were
washed with brine (20 mL), dried (with Na2SO4), and concentrated in vacuo to give a crude product. The crude product
was purified by flash column chromatography on silica gel (n-hexane/ethyl acetate = 2:1) to give the title compound 3c (2.63 g, 30%). Yellow oil; 1H NMR (500 MHz,
CDCl3) δ 4.27–4.14 (m, 4H), 3.93 (q, J = 7.1 Hz, 2H), 3.55 (dt, J = 6.7, 9.0
Hz, 2H), 3.35 (dt, J = 6.7, 9.0 Hz, 2H), 1.61–1.57
(m, 4H), 1.39 (d, J = 7.1 Hz, 6H), 1.35–1.27
(m, 18H including triplet at 1.29 ppm, J = 7.1 Hz,
6H); 13C{1H} NMR (126 MHz, CDCl3)
δ 173.6, 75.0, 70.4, 60.7, 29.7, 29.4, 29.4, 26.0, 18.6, 14.2;
IR (neat) 2985, 2930, 2858, 1739, 1455, 1372, 1263, 1200, 1132, 751
cm–1; HRMS (EI) m/z: [M-C3H5O2]+ calcd for
C17H33O4 301.2379, found 301.2364.
Mizota I., Yamamoto T., Kaliyamoorthy S., Shimizu M., Rathinam B., Liu B.T., Kuroki N., Kiyosawa J, & Tanaka H. (2024). Synthesis of Dicarboxylic Acids Comprising an Ether Linkage and Cyclic Skeleton and Its Further Application for High-Performance Aluminum Electrolyte Capacitors. ACS Omega, 9(9), 10628-10639.
Publication 2024

Top products related to «Decane»

1
Decane by Merck Group
Sourced in Germany, United States, Switzerland
Decane is a straight-chain alkane hydrocarbon with the chemical formula C10H22. It is a colorless, odorless liquid that is insoluble in water. Decane is commonly used as a solvent and in the synthesis of other organic compounds.
2
N decane by Merck Group
Sourced in United States, Germany
N-decane is a linear alkane hydrocarbon compound with the chemical formula C10H22. It is a colorless, volatile liquid at room temperature. N-decane is commonly used as a reference standard and solvent in various laboratory applications.
3
N decane by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany
N-decane is a saturated aliphatic hydrocarbon with the chemical formula C10H22. It is a colorless, flammable liquid with a mild odor. N-decane is commonly used as a solvent and as a reference standard in analytical chemistry applications.
4
Hexadecane by Merck Group
Sourced in United States, Germany, Spain, Italy, United Kingdom, France, Brazil, India, Australia
Hexadecane is a saturated hydrocarbon compound with the chemical formula C16H34. It is a colorless, odorless liquid at room temperature. Hexadecane is commonly used as a reference material and solvent in various laboratory applications.
5
Tetradecane by Merck Group
Sourced in Germany, United States
Tetradecane is a saturated aliphatic hydrocarbon with the chemical formula C14H30. It is a colorless, odorless liquid that is commonly used as a reference material and solvent in various laboratory applications.
6
Nonane by Merck Group
Sourced in Germany, United States, Spain, United Kingdom
Nonane is a saturated aliphatic hydrocarbon with the chemical formula C9H20. It is a colorless liquid with a mild odor. Nonane is used as a reference standard and as a solvent in various laboratory applications.
7
Chloroform by Merck Group
Sourced in United States, Germany, United Kingdom, India, Italy, Spain, France, Canada, Switzerland, China, Australia, Brazil, Poland, Ireland, Sao Tome and Principe, Chile, Japan, Belgium, Portugal, Netherlands, Macao, Singapore, Sweden, Czechia, Cameroon, Austria, Pakistan, Indonesia, Israel, Malaysia, Norway, Mexico, Hungary, New Zealand, Argentina
Chloroform is a colorless, volatile liquid with a characteristic sweet odor. It is a commonly used solvent in a variety of laboratory applications, including extraction, purification, and sample preparation processes. Chloroform has a high density and is immiscible with water, making it a useful solvent for a range of organic compounds.
8
Dodecane by Merck Group
Sourced in Germany, United States
Dodecane is a straight-chain alkane with the chemical formula C12H26. It is a colorless, odorless, and flammable liquid commonly used in laboratory settings as a solvent and reagent.
9
Milli q system by Merck Group
Sourced in United States, Germany, France, United Kingdom, Italy, Morocco, Spain, Japan, Brazil, Australia, China, Belgium, Ireland, Denmark, Sweden, Canada, Hungary, Greece, India, Portugal, Switzerland
The Milli-Q system is a water purification system designed to produce high-quality ultrapure water. It utilizes a multi-stage filtration process to remove impurities, ions, and organic matter from the input water, resulting in water that meets the strict standards required for various laboratory applications.
10
Undecane by Merck Group
Sourced in Germany, Spain, United States
Undecane is a straight-chain alkane hydrocarbon with the chemical formula C₁₁H₂₄. It is a colorless, odorless liquid that is commonly used in various laboratory applications.

More about "Decane"

Decane, also known as n-decane or decyl hydride, is a straight-chain alkane with the molecular formula C10H22.
It is a clear, colorless liquid with a mild, pleasant odor.
Decane is widely used as a solvent, fuel, and in the production of various chemicals.
It can be found naturally in petroleum and is a component of some plant and animal fats.
With a boiling point of 174°C, decane is considered a moderately flammable substance, though it is insoluble in water.
It is, however, miscible with many organic solvents, making it a versatile compound for industrial and research applications.
Closely related aliphatic hydrocarbons, such as hexadecane, tetradecane, nonane, chloroform, dodecane, and undecane, share similar properties and applications with decane.
The Milli-Q system, a widely used water purification method, can be employed to ensure the purity of decane and other solvents used in various experiments and processes.
PubCompare.ai, an AI-driven platform, offers a comprehensive resource for researchers to locate, compare, and optimize protocols involving decane and related compounds, promoting reproducible and effective research.