Log In Sign up
The largest database of trusted experimental protocols
> Chemicals & Drugs > Organic Chemical > Dextran 70

Dextran 70

Dextran 70 is a high-molecular-weight polysaccharide derived from the bacterium Leuconostoc mesenteroides.
It is commonly used as a plasma expander and anticoagulant in medical and research applications.
PubCompare.ai's AI-driven platform can help optimize your Dextran 70 research protocols by locating the best procedures from literature, pre-prints, and patents.
This ensures reproducibility and accuracy, taking the guesswork out of your work and helping you find the most reliable Dextran 70 protocols.
Discvoer the power of PubCompare.ai to enhance your Dextran 70 research today.

Most cited protocols related to «Dextran 70»

1
Measuring Intestinal Permeability with FITC-Dextran
Cited 10 times
Plasma was diluted 1:5 (v/v) in PBS. Gut contents were diluted 1:10 or 1:100 (v/v) with PBS for the 70-kDa or 4-kDa FITC dextran analyses, respectively. Fluorescence was measured spectrophotometrically (Infinite M200 PRO, Tecan, Crailsheim, Germany) in 96-well plates (excitation: 485 nm, emission: 528 nm). FITC dextran concentrations were calculated with the help of standard concentrations prepared in PBS ranging from 0 to 250 µg/mL 4-kDa FITC dextran or 0 to 1250 µg/mL 70-kDa FITC dextran. Emission signals in plasma and gut contents of the mice receiving PBS were subtracted from those of mice treated with the 4-kDa or the 70-kDa FITC dextran. The fluorescence signal of luminal 70-kDa FITC dextran in each segment was related to the sum of the fluorescence signals in all segments of the gastrointestinal tract.
Woting A, & Blaut M. (2018). Small Intestinal Permeability and Gut-Transit Time Determined with Low and High Molecular Weight Fluorescein Isothiocyanate-Dextrans in C3H Mice. Nutrients, 10(6), 685.
Publication 2018
Dextran 70 Fluorescein-5-isothiocyanate fluorescein isothiocyanate dextran Fluorescence Gastrointestinal Tract M-200 Mus Phenobarbital Plasma
2
Imaging Techniques for Studying Mouse Cortex
Cited 10 times
The thin skull procedure was used for acute and chronic trans-cranial
imaging as described previously17 (link) while the cranial window procedure was used for acute
imaging sessions with dye labeling. Briefly, mice were fully anesthetized using
isoflurane (MBP shiverer) or Ketamine/Xylazine, and the scalp
was shaved and sterilized. A midline scalp incision was made, and a custom made
metal plate was affixed to the skull using cyanoacrylate. An area no more than
1mm was thinned with a high speed drill and a microsurgical blade to a thickness
of 20–30 μm, or removed along with the underlying dura for the
cranial window. For fluorescent myelin labeling, Fluoromyelin (Life
Technologies) was applied directly to the exposed cortex in a 50%
dilution in PBS from stock solution for 45 minutes and then washed thoroughly.
Occasionally, we observed reflective fibers that were partially labeled with
Fluoromyelin which was likely due to regional variation in dye penetration
resulting in incomplete dye labeling. Cortical vasculature was visualized with
intravenous injection of 70,000 MW Texas red dextran (Life Technologies). For
astrocyte labeling 50 μM sulforhodamine 10143 (link) dissolved in PBS was applied for 20
minutes to the exposed cortex and then washed thoroughly. A #0 glass
coverslip cut to size was placed over the cranial window and glued in place
using cyanoacrylate.
Schain A.J., Hill R.A, & Grutzendler J. (2014). Label-free in vivo imaging of myelinated axons in health and disease with spectral confocal reflectance microscopy. Nature medicine, 20(4), 443-449.
Publication 2013
Cortex, Cerebral Cranium Cyanoacrylates Dextran 70 Drill Dura Mater Ketamine Kidney Cortex Mice, House Myelin Scalp Technique, Dilution Xylazine
3
Hypertonic Saline vs. Normal Saline for TBI
Cited 10 times
This study was conducted by the Resuscitation Outcomes Consortium, a multicenter clinical trial network including 11 regional clinical centers in the United States and Canada. The trial involved 114 emergency medical services agencies within the catchment area served by the consortium.10 (link) Two trials with 2 distinct patient cohorts, one for hypovolemic shock and the other for TBI, were conducted simultaneously using the same intervention.
This report describes the outcome of the TBI cohort. This was a double-blind, 3-group, randomized controlled clinical trial comparing a 250-mL bolus of 7.5% saline (hypertonic saline) vs 7.5% saline/6% dextran 70 (hypertonic saline/dextran) vs 0.9% saline (normal saline) as the initial resuscitation fluid administered to injured patients with suspected severe TBI in the out-of-hospital setting. This dose of hypertonic saline and hypertonic saline/dextran was selected because it was the dose used in all previous prehospital trials and thus had a proven safety record. Previous studies suggested that the expected serum sodium level on admission would be 145 to 155 mEq/L. Details of the initial study design have been published.11 (link)
Bulger E.M., May S., Brasel K.J., Schreiber M., Kerby J.D., Tisherman S.A., Newgard C., Slutsky A., Coimbra R., Emerson S., Minei J.P., Bardarson B., Kudenchuk P., Baker A., Christenson J., Idris A., Davis D., Fabian T.C., Aufderheide T.P., Callaway C., Williams C., Banek J., Vaillancourt C., van Heest R., Sopko G., Hata J.S, & Hoyt D.B. (2010). Out-of-Hospital Hypertonic Resuscitation Following Severe Traumatic Brain Injury: A Randomized Controlled Trial. JAMA : the journal of the American Medical Association, 304(13), 1455-1464.
Publication 2010
Dextran Dextran 70 Hypovolemic Shock Normal Saline Patients Resuscitation Safety Saline Solution Saline Solution, Hypertonic Serum Service, Emergency Medical Sodium
4
Assessing Blood-Brain Barrier Permeability
Cited 8 times
Dextran-rhodamine 70-kDa (RHO70; 10mg/mL, 2.86µl/g/each; Sigma-aldrich, MO) and dextran-FITC 10-kDa (FITC10, 10mg/mL, 2.86µl/g/each; Sigma-aldrich, MO), or the fluorescently-labeled angiotensin II (AngIIfluo, 1µmol/L, 2.86µl/g/each; Anaspec, CA), were delivered intracarotidly, and allowed to circulate for 20 min. When indicated, hypertonic mannitol (1.4M, 2ml per 200–250g of animal; Sigma-aldrich, MO)22 (link)–24 (link) was infused intracarotidly 5 min prior to the dextrans. Hypothalamic and brainstem sections (40µm) were counterstained (Toto 1:50000).
Immunofluorescence (Table.S1) was performed for cell-type identification, and examined by confocal microscopy (LSM510, Zeiss Microimaging)25 (link). BBB permeability was assessed based on the detection of fluorescent-dye staining within brain microvasculature or parenchyma26 (link). The extravasated FITC10 signal was isolated using a digital subtraction procedure, and the proportion of FITC10-stained pixels in the subtracted image was calculated and compared among experimental groups. A densitometry analysis was used to compare differences in BBB protein constituents or immunoreactivity between WKYs and SHRs and results were expressed as % area within the nucleus occupied by the detected immunoreactivity25 (link).
Biancardi V.C., Son S.J., Ahmadi S., Filosa J.A, & Stern J.E. (2013). CIRCULATING ANGIOTENSIN II GAINS ACCESS TO THE HYPOTHALAMUS AND BRAINSTEM DURING HYPERTENSION VIA BREAKDOWN OF THE BLOOD BRAIN BARRIER. Hypertension, 63(3), 572-579.
Publication 2013
Angiotensin II Animals Brain Brain Stem Cell Nucleus Cells Densitometry Dextran Dextran 70 Dextrans Fingers Fluorescein-5-isothiocyanate Fluorescent Antibody Technique Fluorescent Dyes Hypothalamus Mannitol Microscopy, Confocal Microvascular Network Permeability Proteins Rhodamine SHORT ROOT protein, Arabidopsis TOTO-1 thiazole orange dye
5
In vivo Imaging of Cortical Neurites
Cited 7 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2019
Blood Vessel blue dextran Cortex, Cerebral Craniotomy Cranium Dendrites Dextran 70 Dura Mater Fibrinogen Lens, Crystalline Light Microscopy Mice, House Microglia Microscopy Microtubule-Associated Proteins Neurites Operative Surgical Procedures Plasma Pressure rhodamine dextran Vertebral Column Vision

Most recents protocols related to «Dextran 70»

1
Lysosomal pH Estimation via Fluorescent Dextrans
To estimate the relative lysosomal pH, subconfluent cells were loaded with 2.5 mg/mL pH-sensitive FITC coupled to 70 kDa dextran and 2.5 mg/mL pH-insensitive tetramethylrhodamine (TMR) coupled to 70 kDa dextran for 18 h, washed, and chased in fresh medium for 5 h. The medium was changed to Live Cell Imaging Solution (Thermo Fisher Scientific, Cat. A14291DJ) before image acquisition by LSM700 Confocal Laser Scanning Microscope (Zeiss). Images were analyzed using ImageJ software.
Liu B., Chen R., Zhang Y., Huang J., Luo Y., Rosthøj S., Zhao C, & Jäättelä M. (2023). Cationic amphiphilic antihistamines inhibit STAT3 via Ca2+-dependent lysosomal H+ efflux. Cell Reports, 42(2), 112137.
Publication 2023
Cells Dextran 70 Fluorescein-5-isothiocyanate Lysosomes Microscopy, Confocal, Laser Scanning tetramethylrhodamine
2
Isolation of Retinal and Brain Microvessels
The rat RMVs and BMVs were isolated as described previously.11 (link) Briefly,
brain and retinal crysections were individually homogenized using a motor-driven
homogenizer (Homgen plus, Schuett Biotec, Goettingen, Germany). The brain
homogenate was centrifuged at 438g for 10min, followed by centrifugation at
4400g for 15min, after which the pellet was resuspended into 7 mL PBS/1% dextran
(Dextran 70,000, Roth). Thereafter, the brain and retinal suspension were
individually transferred onto a density gradient column and centrifuged for 15
min (1300 g). Finally, the microvessels were captured after filtration over a
60 µm nylon mesh. All the procedures were performed at 0°C.
Li Y., Faiz A., Moshage H., Schilling L, & Kamps J.A. (2023). Responses of retinal and brain microvasculature to streptozotocin induced diabetes revealed by global expression profiling. Diabetes & Vascular Disease Research, 20(1), 14791641221147533.
Publication 2023
Brain Centrifugation dextran 1 Dextran 70 Filtration Microvessels Nylons Retina
3
Visualizing Vascular Leakage in Brain Tumors
A bolus of 100 μl of a 10 mg ml–1 70 kDa dextran-TMR solution (Life Technologies) was injected via the tail vein into tumour-bearing SHH-MB mice at advanced symptomatic stages, as previously described6 (link). Brains were then removed 2 h later without perfusion, fixed overnight in 4% paraformaldehyde, embedded in optimal cutting temperature compound (O.C.T.) and then sections prepared at a thickness of 12 μm. Immunofluorescent staining of tissue sections was performed using antibodies against CD31 and P-selectin with appropriate secondary antibodies, counterstained with DAPI to visualize nuclei and then coverslipped using Fluoro-Gel mounting medium as described below. Detection of TMR-dextran in the context of P-selectin and CD31 immunostaining was imaged using a fluorescent microscope (Zeiss Axioobserver), and TIFF images postprocessed using Adobe Photoshop CS6.
Tylawsky D.E., Kiguchi H., Vaynshteyn J., Gerwin J., Shah J., Islam T., Boyer J.A., Boué D.R., Snuderl M., Greenblatt M.B., Shamay Y., Raju G.P, & Heller D.A. (2023). P-selectin-targeted nanocarriers induce active crossing of the blood–brain barrier via caveolin-1-dependent transcytosis. Nature Materials, 22(3), 391-399.
Publication 2023
A-A-1 antibiotic Antibodies Brain Cell Nucleus DAPI Dextran Dextran 70 Fluorescent Antibody Technique Mice, House Microscopy Neoplasms paraform Perfusion SELP protein, human Tail Tissue Stains Veins
4
Isolation of Brain Microvessels for Omics
Brain microvessels were isolated from the ischemic hemispheres of young and old C57BL/6 mice at 7 days post-TE stroke, as well as corresponding hemispheres in sham operated mice using an established protocol [17 (link), 52 (link)]. Briefly, brain tissue was minced in Dulbecco’s Phosphate Buffered Saline (DPBS, Life Technology Corporation, Grand Island, NY USA) and homogenized gently in a Dounce glass homogenizer. Myelin was removed by centrifugation in a 15% Dextran solution (Dextran MW ∼70,000, Sigma Aldrich, St Louis, MO USA). The obtained pellet was transferred to 40 mm cell strainer and washed by DPBS supplemented by 0.5% endotoxin-, fatty acid- and protease free bovine serum albumin (BSA, Sigma-Aldrich, St Louis MO USA) to retrieve microvessels. The isolated blood vessels were then digested for 5 min with 0.25% trypsin (Life Technology Corporation, Grand Island, NY USA) at 37 °C to remove perivascular cells. Microvessels’ purity was evaluated by immunocytochemistry using anti-CD31 (brain endothelial cells; BD Bioscience), GFAP (astrocytes; Sigma Aldrich, St Louis, MO USA), PDGFRβ (pericytes; Abcam, Waltham, MA, USA), and Iba1 (microglia; Abcam, Waltham, MA, USA) antibodies. The protocol produced 99.99% “clean” (without perivascular cells) blood vessels. Isolated brain microvessels were further processed for paired-end mRNA-sequencing and reduced representation bisulfite sequencing for transcriptome and DNA methylome analyses, respectively.
Phillips C.M., Stamatovic S.M., Keep R.F, & Andjelkovic A.V. (2023). Epigenetics and stroke: role of DNA methylation and effect of aging on blood–brain barrier recovery. Fluids and Barriers of the CNS, 20, 14.
Publication 2023
Antibodies Astrocytes Blood Vessel Brain Cells Centrifugation Cerebrovascular Accident Dextran Dextran 70 Endothelial Cells Endotoxins Epigenome Fatty Acids Glial Fibrillary Acidic Protein hydrogen sulfite Immunocytochemistry Mice, Inbred C57BL Microglia Microvessels Mus Myelin Peptide Hydrolases Pericytes Phosphates Platelet-Derived Growth Factor beta Receptor RNA, Messenger Saline Solution Serum Albumin, Bovine Tissues Trypsin
5
Microfluidic Encapsulation of Catalase
All solutions were flushed with nitrogen for at least 0.5 h, and the fluorocarbon oil (HFE 7500) was flushed for 15 min, to remove dissolved oxygen. Dextran (20% w/w) and PEGDA (40% w/w) were independently injected in the first cross-junction. The photo-initiator was added (0.4 wt% final concentration) to the PEGDA solution prior to injection. The droplets were formed at the second cross-junction by the introduction of an outer phase which consisted of a fluorocarbon oil (HFE 7500) and a surfactant (SS08, 2% w/w). The resulting emulsion was collected in an Eppendorf. UV curing of PEGDA was achieved by exposing the emulsion to a focused UV beam (λ = 300–600 nm, 5 min, 20% intensity). The emulsion was broken by adding 1H,1H,2H,2H-Perfluoro-1-octanol (100μL, 20% w/w in hexane), after which the beads were washed rigorously with MiliQ to ensure that no surfactant remained.
Catalase (>20,000 u/mg) was coupled with Alexa Fluor®®® 47 dye as described in the manual and was added prior to injection, with 6 mg/mL and 18 mg/mL for the PEGDA and polysaccharide phase, respectively. The volume ratio of PEGDA:polysaccharide is roughly 9:1, taking into account the relative concentrations, and the overall catalase content is three times higher in the homogeneous system compared to the localized system. Flowrates: Oil: 600 µL/h, PEGDA: 60 µL/h, Dextran 10 kDa: 20 µL/h, Dextran 70 kDa and Ficoll 400 kDa: 10 µL/h.
Keller S., Teora S.P., Keskin A., Daris L.J., Samuels N.A., Boujemaa M, & Wilson D.A. (2023). Spatial Control over Catalyst Positioning for Increased Micromotor Efficiency. Gels, 9(2), 164.
Publication 2023
Catalase Dextran Dextran 70 Emulsions Ficoll Fluorocarbons HFE-7500 n-hexane Nitrogen Octanols Oxygen poly(ethylene glycol)diacrylate Polysaccharides Surfactants

Top products related to «Dextran 70»

1
Dextran 70 by Merck Group
Sourced in United States
Dextran 70 is a type of polysaccharide polymer derived from the bacterium Leuconostoc mesenteroides. It is a biocompatible and water-soluble substance commonly used in laboratory applications. Dextran 70 has a molecular weight of approximately 70 kilodaltons and is primarily used as a volume expander and as a component in various cell culture media and buffer solutions.
2
Texas red dextran by Thermo Fisher Scientific
Sourced in United States, Germany, Switzerland
Texas Red dextran is a fluorescent dye conjugated to a dextran polymer. It is used as a molecular tracer and fluorescent label for various biological applications.
3
Texas red by Thermo Fisher Scientific
Sourced in United States, United Kingdom
Texas Red is a fluorescent dye that can be used to label proteins, nucleic acids, and other biological molecules. It has an excitation maximum at 596 nm and an emission maximum at 615 nm, making it suitable for use with common fluorescence detection equipment.
4
Texas red 70 kda dextran by Thermo Fisher Scientific
Sourced in Canada, United States
Texas Red 70 kDa dextran is a fluorescent labeling compound used in various biological applications. It has a molecular weight of approximately 70,000 Daltons and is conjugated with the Texas Red fluorescent dye. This product can be used to label and track the movement or distribution of molecules, cells, or other biological entities in experimental research settings.
5
Texas red labeled dextran 70 by Thermo Fisher Scientific
Texas Red-labeled dextran-70 is a fluorescent labeling reagent used in molecular and cellular biology applications. It consists of a dextran molecule (70 kDa) conjugated to the Texas Red fluorescent dye. The Texas Red label provides a red fluorescent signal that can be detected and imaged using appropriate instrumentation and techniques.
6
Dextran texas red 70 000 mw by Thermo Fisher Scientific
Dextran Texas-Red 70 000 MW is a high molecular weight dextran conjugated with the Texas Red fluorescent dye. It has a molecular weight of 70,000 Daltons.
7
Fitc dextran 70 by Merck Group
FITC-dextran 70 is a fluorescently-labeled polysaccharide compound used for various research applications. It is composed of dextran molecules conjugated with fluorescein isothiocyanate (FITC), a common fluorescent dye. The FITC-dextran 70 product has a molecular weight of approximately 70,000 Daltons. Its core function is to serve as a tracer and marker in experimental studies, allowing researchers to visualize and track the movement and distribution of molecules or particles in various biological systems.
8
Fitc dextran by Merck Group
Sourced in United States, Germany, United Kingdom, Sao Tome and Principe, Japan, China, Macao, Spain, Switzerland, France, Italy, Australia, Israel, Sweden, Belgium, Canada
FITC-dextran is a fluorescent labeled dextran compound. It is a water-soluble carbohydrate polymer that is covalently linked to the fluorescent dye fluorescein isothiocyanate (FITC). FITC-dextran is commonly used as a tracer or marker in various biological applications.
9
Tmr dextran 70 kda by Thermo Fisher Scientific
Sourced in United States
TMR-dextran 70 kDa is a fluorescently labeled dextran polymer with a molecular weight of 70 kilodaltons. It is a water-soluble carbohydrate commonly used as a tracer or marker in biological research applications.
10
Glyceraldehyde 3 phosphate dehydrogenase antibody by Cell Signaling Technology
Sourced in United States
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a housekeeping enzyme involved in glycolysis. The GAPDH antibody is a tool used to detect and quantify GAPDH protein expression in biological samples.

More about "Dextran 70"

Dextran 70 is a high-molecular-weight polysaccharide derived from the bacterium Leuconostoc mesenteroides.
This versatile compound is commonly used as a plasma expander and anticoagulant in various medical and research applications.
Beyond Dextran 70, there are several related terms and compounds worth exploring: Texas Red dextran, Texas Red, and Texas Red 70 kDa dextran are fluorescently labeled variants of Dextran 70, often utilized in cellular and vascular imaging studies.
Similarly, Texas Red-labeled dextran-70 and Dextran Texas-Red 70 000 MW provide fluorescent labeling options for tracking and visualizing Dextran 70 in biological systems.
FITC-dextran 70 and FITC-dextran are additional fluorescently tagged dextran compounds, where FITC (fluorescein isothiocyanate) serves as the fluorescent marker.
These can be used interchangeably with Texas Red-labeled dextrans for various research applications.
TMR-dextran 70 kDa is another fluorescent dextran derivative, with tetramethylrhodamine (TMR) as the fluorescent label.
This compound offers an alternative to the Texas Red and FITC-labeled versions for specialized experiments.
Glyceraldehyde-3-phosphate dehydrogenase antibody, while not directly related to Dextran 70, can be a useful tool in research studies involving cellular processes and metabolism, which may complement the use of Dextran 70 as a plasma expander or vascular tracer.
PubComapre.ai's AI-driven platform can help optimize your Dextran 70 research protocols by locating the best procedures from literature, pre-prints, and patents.
This ensures reproducibility and accuracy, taking the guesswork out of your work and helping you find the most reliable Dextran 70 protocols.
Discvoer the power of PubCompare.ai to enhance your Dextran 70 research today.