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Dextrin

Dextrin is a complex carbohydrate derived from the partial hydrolysis of starch.
It is commonly used as a food additive, adhesive, and in pharmaceutical applications.
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Most cited protocols related to «Dextrin»

Sulfolobus acidocaldarius DSM 639, MW001, and all constructed deletion mutants were aerobically grown in Brock media (Brock et al., 1972 (link)) with a pH of 3 at 76°C. The media were supplemented with 0.1% (w/v) tryptone or with 0.1% (w/v) N-Z-Amine and 0.2% dextrine. The growth of the cells was monitored by measurement of the optical density at 600 nm.
For pouring Sulfolobus plates a two times concentrated Brock media was supplemented with 6 mM CaCl2 and 20 mM MgCl2. For first selection plates 0.2% NZ-Amine (Fluka) and 0.4% dextrin, for second selection plates 0.2% tryptone, 0.4% dextrin, 200 μg/ml 5-FOA, and 20 μg/ml uracil was added to the two times concentrated solution and prewarmed to 75°C. This solution was mixed with an equal volume of fresh boiling 1.4% Gelrite solution (Carl Roth, Karlsruhe, Germany) and poured in 40 ml portions into petri dishes (150 × 20 mm, Sarstedt, Nümbrecht, Germany).
Publication 2012
Amines Deletion Mutation Dextrin Gelrite Hyperostosis, Diffuse Idiopathic Skeletal Magnesium Chloride Sulfolobus Sulfolobus acidocaldarius Uracil
Timed pregnant Sprague-Dawley dams were obtained from the Center for Behavioral Teratology, San Diego State University Animal Care facilities. Gestational day (GD) 0 was designated as the day when a seminal plug was detected. Dams were housed individually in plastic cages, exposed to a 12:12 hour cycle of light and dark in a temperature- and humidity-controlled room, and received food (LabDiet® 5001, Richmond, IN, containing 2.25 g choline chloride/kg diet) and water ad libitum.
Pregnant dams were randomly assigned to one of three treatment groups: ethanol-exposed (EtOH), yoked pair-fed (PF), or ad libitum control (LC). Ethanol-exposed dams received 6.0 g/kg/day (28.5% v/v) ethanol, PF dams received an isocaloric maltose dextrin solution to control for the calories from alcohol, and LC dams received vehicle (saline), via daily oral gavage from GD 5–20. Daily food intake was measured for the EtOH dams; each PF dam was matched to an EtOH dam of similar weight and food intake was correspondingly yoked. Within each of the 3 treatment groups, dams were randomly assigned to receive either a choline supplementation (choline chloride, Blachem, New Hampton, NY; 250 mg/kg/day) or a vehicle control (saline, Sigma, Milwaukee, WI), added to the daily intubation formula. This administration increases daily choline intake by 2–3 times that of controls.
Animals were monitored until the expected day of delivery GD 22 (PD 0) and the day of birth was recorded. On PD 1, litters were culled to 10 pups (5 males and 5 females when possible). Data on litter characteristics and birth weights have been previously reported (Thomas et al., 2009 (link)). Blood alcohol levels over a 24-hour period were obtained from a separate group of pregnant rats on gestational days 5 and 20. Importantly, choline supplementation did not influence blood alcohol concentrations, which peaked at 345 mg/dL (Thomas et al., 2009 (link)), indicating that choline does not alter the amount of fetal alcohol exposure.
Publication 2010
Animals Birth Choline Choline Chloride Dextrin Diet Eating Ethanol Females Fetus Food Humidity Intubation Males Maltose Obstetric Delivery Patient Holding Stretchers Pregnancy Rattus norvegicus Saline Solution Tube Feeding

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Publication 2012
Animals Animals, Laboratory Birth Body Weight Brain Decapitation Dextrin Eating Embryo Ethanol Females Males Maltose Patient Holding Stretchers Rats, Sprague-Dawley Rattus norvegicus Rivers Rodent Spontaneous Abortion Tissues Tube Feeding Visually Impaired Persons
Male Fischer 344 rats (~ 6 weeks old) purchased from Harlan (Indianapolis, IN) were housed in a humidity and temperature controlled animal room with automatic 12h light/dark cycles. After one week, animals were housed individually in cages and provided with regular Lieber-DeCarli liquid diet (cat. no. 710260) [carbohydrates (11% of calories), protein (18%), fat (35%) and ethanol or maltose dextrin (36%) (Lieber and DeCarli, 1989 (link))] (Dyets Inc., Bethlehem, PA) for a week. Liquid diets containing ethanol or equivalent calories substituted by maltose-dextrin (cat. no, 402851) for pair-feeding were prepared according to manufacturers instructions. Pair-feeding of animals was done to minimize confounding variables such as weight gain and caloric intake between control and experimental rats.
The amount of ethanol in the liquid diet was increased gradually from 1% to 5% and then maintained at 5% for one month as described earlier (Bhopale et al., 2006 (link)). The diets were prepared and changed daily and weights of animals were recorded each week. Daily intake of liquid diet for each animal, their general health, and any signs of distress and morbidity were recorded.
Animals from the control and ethanol fed group were sacrificed on the 30th day by an intraperitonial injection of Nembutol (sodium salt, 80 mg/ml) and blood from the heart was collected in heparinized tubes, centrifuged at 1,000 g for 10 minutes and plasma separated and stored at −80 °C.
Liver was obtained, blotted, weighed and a portion of the liver from each animal was fixed in 10% formalin for histology using Hemotoxylin and Eosin (H&E) staining. Remaining liver was stored at −80 °C for biochemical analysis. Oil Red O staining was done on frozen liver sections. Malondialdehyde (MDA) quantification was performed using the Oxiselect TBARS assay kit STA-330 (Cell Biolabs, Inc. CA). Staining for 4-hydroxynonenal (HNE) was performed using anti-4HNE antibody HNE11-S (Alpha Diagnostics, San Antonio, TX). Staining for T-cells were performed using rabbit anti mouse CD3 antibodies (Dako, Carpinteria, CA).
Publication 2010
Animals Antibodies Antibodies, Anti-Idiotypic Biological Assay BLOOD Carbohydrates Cells Dextrin dextrin maltose Diagnosis Diet Eosin Ethanol Formalin Frozen Sections Heart Hematoxylin Humidity Liver Males Malondialdehyde Maltose Mice, House Muromonab-CD3 Plasma Proteins Rabbits Rats, Inbred F344 Rattus norvegicus Sodium Sodium Chloride, Dietary T-Lymphocyte Thiobarbituric Acid Reactive Substances

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Publication 2010
Adrenal Cortex Hormones Animals Cereals Circadian Rhythms Dextrin Diet Eating Ethanol Females Human Body Lactation Males Maltose N-(2,4-dinitroanilino)maleimide Patient Holding Stretchers Pinus polycarbonate Pregnancy Pregnant Women

Most recents protocols related to «Dextrin»

DEXGEL Bone is an injectable, porous, and osteoconductive bone substitute composed of two phases: (i) dextrin hydrogel matrix, DEXGEL — polymeric phase and (ii) BL®, composed mostly of hydroxyapatite, having a percentage of tricalcium phosphate — ceramic phase. The DEXGEL Bone kit is composed of (i) four vials: one with the ODEX solution, another with ADH (both dissolved in PBS) and 2 vials of BL® with 0.5 g each; (ii) a syringe with needle, to transfer ADH solution and subsequent mixing of all components; and (iii) a second syringe to apply the final formulation into the alveolar socket. Components must be mixed at the time of surgery by the medical team, according to a specific protocol (Fig. 1). Each device was prepared for a final volume of 2 cm3, including 0.4 mL of ADH, 0.933 mL of ODEX, and 1 g of BL®. BL® granules, 50% v/v of the total volume of the final formulation (HG), were mixed with the ODEX solution, and the reticulation was achieved adding the ADH solution prior to surgery (Fig. 1), in a proportion of 7:3 (ODEX:ADH) volume ratio. DEXGEL Bone was ready for administration in a pre-gelled moldable form 5 min upon ADH addition.

DEXGEL Bone preparation procedure

Figure 2 shows the necessary time for device handling at the time of surgery, from components mixing to implantation. The preparation was performed by a technician the moment surgeon began the extraction process. Exodontia generally took 5 to 10 min, which matched the time needed for device preparation. After properly gelled in the form of a moldable paste, DEXGEL Bone could be readily implanted or either rest for an extended period of 2 h before implantation, as most convenient to the surgeon. Within this period, the hydrogel matrix did not dehydrate, thus not compromising granule stabilization. Sculpting could be performed from 1 to 2 min. There was no need for a set time within the defect as in other grafting materials, the gum tissue can therefore be closed right after sculpting.

Timeline for DEXGEL Bone preparation and handling

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Publication 2023
Bones Bone Substitutes Cytoplasmic Granules Dextrin Durapatite Gomphosis Medical Devices Needles Operative Surgical Procedures Osteoconduction Ovum Implantation Pastes PEGDMA Hydrogel Polymers Reticulum Surgeons Syringes Tissues Tooth Extraction tricalcium phosphate ceramic
Dextrin oxidation was performed as previously described [32 (link)]. Briefly, aqueous solutions of dextrin (2% w/v) were oxidized with sodium m‐periodate (NaIO4), to yield the theoretical degree of oxidation of 40%, at room temperature, with stirring, in the dark. The oxidation reaction was stopped after 20 h by dropwise addition of an equimolar amount of diethylene glycol to reduce any unreacted periodate. Sodium m‐periodate and diethylene glycol were removed by dialysis, using a 1000-Da cutoff membrane (Merck Millipore, Billerica, MA, USA), and then freeze-dried. ODEX was dissolved in PBS solution (30% w/v) and sterilized by gamma irradiation (IONISOS, Dagneux, France), using a 60Co source, at 20 kGy (2 kGy/h), at room temperature. ADH was also dissolved in PBS solution (3.76% w/v) and sterilized by filtration, using a 0.22-μm pore filter membrane (Pall Corporation, Ann Arbor, MI, USA). For the crosslinking reaction, ODEX and ADH solutions were mixed in a 7:3 volume ratio, respectively. ODEX and ADH were packaged in separate microtubes, vacuum sealed, and stored at 4 °C. Three samples of each were analyzed for endotoxin content by Biogerm, S.A. (Moreira, Portugal) and sterility by Sagilab Laboratório de Análises Técnicas, S.A. Heat-resistant laboratory materials were sterilized by autoclave, and heat-sensitive materials were sterilized by ethylene oxide. The sterilization process and subsequent sterility validation were performed according to the ISO 11137:2006 [33 ] and ISO 11737: 2009 [34 ] requirements, respectively.
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Publication 2023
Dextrin Dialysis diethylene glycol Endotoxins Filtration Freezing Gamma Rays metaperiodate Oxide, Ethylene Radiotherapy sodium metaperiodate Sterility, Reproductive Sterilization Tissue, Membrane Vacuum

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Publication 2023
AN 12 BLOOD brexanolone Citrates Dextrin Hospitalization Patient Discharge Patients Phlebotomy Pregnanolone, (3beta, 5alpha)-isomer Sterility, Reproductive
Xin'an Jianpi Tongbi prescription is a compound preparation of TCM based on the Xin'an medical theory. It contains Xinfeng capsule [22 (link)] (Z20050062 from Wanyao Pharmaceutical Co., Ltd., patent number: ZL 2013 1 0011369.8), Huangqin Qingre Chubi capsule [24 ] (Z20200001 from Wanyao Pharmaceutical Co., Ltd., patent number: ZL 2011 1 0095718.X), and Wuwei Wentong Chubi capsule [25 (link)] (patent number: ZL 2020 10714863.0). The Xinfeng capsule is composed of Astragalus membranaceus, Semen coicis, Tripterygium wilfordii, and Scolopendra spp. These four medicinal materials were extracted by refluxing twice with 75% ethanol. In the first step, ten times the amount of ethanol was added for extraction for 2 h, after which eight times the amount of ethanol was added and allowed to extract for 1.5 h. The drug residues were boiled with eight times the amount of water and extracted for 1.5 h. This was then filtered and allowed to stand. The supernatant was collected and combined with the alcohol extract under pressure to concentrate, and the paste was collected. The sample was dried, crushed, mixed with dextrin, and granulated with ethanol. This was followed by drying, whole granulating, sterilizing, filling, and outsourcing. The Huangqin Qingre Chubi capsule is composed of Scutellaria baicalensis, Prunus persica, Gardenia jasminoides, Semen coicis, and Clematis chinensis. These five medicinal materials were decocted and extracted three times as follows: ten times the amount of water was added for the first time and extracted for 1.5 h; eight times the amount of water was added for the second and third times and extracted for 1 h. The mixture was strained and allowed to stand. Then, the supernatant was absorbed and concentrated under pressure, and the paste was collected; this was then vacuum-dried, the dry extract was crushed, and dextrin was added. Ethanol was used to soften the materials, which were screened, granulated, dried, whole-grained, and filled into capsules. The Wuwei Wentong Chubi capsule is composed of Poria cocos, Epimedium brevicornu, Cinnamomum cassia, Curcumae Longae, and Scutellaria baicalensis. These five medicinal materials were decocted and extracted three times as follows: ten times the amount of water was added for the first time and extracted for 1.5 h; eight times the amount of water was added for the second and third times and extracted for 1 h. This mixture was strained and allowed to settle. Then, the supernatant was absorbed and concentrated under reduced pressure, and the paste was collected. This was then vacuum-dried, the dry extract was crushed, and dextrin was added. Ethanol was used to soften the material, which was then sieved using no. 12 mesh, granulated, dried, whole-grained, filled into capsules, and outsourced. All capsules were produced by the preparation center of the First Affiliated Hospital of Anhui University of Chinese medicine, and the variation range of each capsule was ±10%.
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Publication 2023
11-dehydrocorticosterone Astragalus membranaceus Capsule Chinese Cinnamomum cassia Clematis Dextrin Epimedium Ethanol Gardenia Huangqin Paste Pharmaceutical Preparations Plant Embryos Pressure Prunus persica Scolopendra Scutellaria baicalensis extract Tripterygium wilfordii Vacuum Wolfiporia extensa xinfeng
WT, Ube3am–/p+ (maternal transmission), and Ube3am+/p– (paternal transmission) mice (6-8 weeks old) were pair-fed for 8-12 weeks (before DRG dissection or behavioral experiments) with a: A) standard diet (sd; #7012 ENVIGO; UTHSC animal facility diet; (https://insights.envigo.com/hubfs/resources/data-sheets/7012-datasheet-0915.pdf); B) Safflower oil-supplemented diet (enriched in LA, Dyets # 112245). Modified AIN-93G purified rodent diet with 59% fat derived calories from safflower oil (kcal/kg): Casein (716), L-Cystine (12), Maltose Dextrin (502), Cornstarch (818.76), Safflower Oil (2430), Soybean Oil (630), Mineral Mix (# 210025; 30.8), and Vitamin Mix (#310025; 38.7); C) High-fat diet (anhydrous milk fat supplemented, Dyets # 105012). Modified AIN-93G Purified Rodent Diet with 59% Fat Derived Calories from Anhydrous Milk Fat (kcal/kg): Casein (716), L-Cystine (12), Maltose Dextrin (502), Cornstarch (818.76), Anhydrous Milk Fat (2430), Soybean Oil (630), Mineral Mix (# 210025; 30.8), and Vitamin Mix (#310025; 38.7).
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Publication 2023
Animals Caseins Cornstarch Cystine Dextrin Diet, High-Fat Dietary Fats Dissection Maltose Mice, House Milk, Cow's Minerals Rodent Safflower oil Soybean oil Therapy, Diet Transmission, Communicable Disease Vertical Infection Transmission Vitamins

Top products related to «Dextrin»

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C57BL/6J mice are a widely used inbred mouse strain. They are a commonly used model organism in biomedical research.
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Dextrin is a polysaccharide derived from the hydrolysis of starch. It is used as a stabilizing and thickening agent in various laboratory applications.
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Maltose dextrin is a carbohydrate compound used in various laboratory applications. It serves as a stabilizing agent, bulking agent, and excipient in the formulation and preparation of various products.
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The F1258SP is a compact digital dry bath incubator designed for precise temperature control of samples. It features a stable temperature range from ambient +5°C to 150°C. The unit has a compact footprint and an LED display for easy monitoring of temperature.
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The Lieber-DeCarli liquid diet is a laboratory equipment product designed to provide a controlled and standardized liquid diet for experimental use. It is used to administer a pre-determined nutritional formula to research subjects. The core function of this product is to enable the delivery of a consistent and measurable liquid diet in a laboratory setting.
Sourced in United States, Germany, United Kingdom
Tissue culture flasks are laboratory containers designed for the in vitro cultivation of cells, tissues, or organisms. They provide a controlled environment for cell growth and experimentation, facilitating the study and manipulation of biological samples.
The Lieber-DeCarli '82 Shake and Pour control liquid diet is a laboratory equipment product designed for the controlled feeding of liquid diets. It provides a standardized liquid diet that can be used in research applications.
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Resazurin sodium salt is a chemical compound commonly used as an indicator in various laboratory applications. It is a redox-sensitive dye that changes color based on the oxidation-reduction state of the surrounding environment. When used in cell culture systems, resazurin can be employed as a viability and cytotoxicity assay to measure cellular metabolic activity.
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N-Z amine is a laboratory reagent used in the synthesis and purification of organic compounds. It is a versatile amine-containing compound that can be used in various chemical reactions and processes. The core function of N-Z amine is to serve as a building block or intermediate in the production of more complex organic molecules.

More about "Dextrin"

Dextrin is a complex carbohydrate that is commonly used in various applications, including food, adhesives, and pharmaceuticals.
It is derived from the partial hydrolysis of starch, a process that breaks down the large starch molecules into smaller, more manageable units.
Dextrin is closely related to other carbohydrates, such as maltose dextrin, which is a mixture of dextrin and maltose.
These carbohydrates are often used interchangeably in research and industry, and understanding their properties and applications can be crucial for researchers and scientists.
In the context of scientific research, dextrin and its related compounds have been studied extensively, particularly in the field of animal models.
C57BL/6J mice, for example, are a widely used strain in biomedical research, and dextrin-based diets, such as the Lieber-DeCarli liquid diet, have been employed to study the effects of alcohol consumption and other metabolic processes.
Additionally, dextrin and its derivatives have been utilized in tissue culture applications, where they can serve as growth substrates or nutrient sources for cell cultures.
The Resazurin sodium salt, for instance, is a dye that is often used to assess cell viability and proliferation in these settings.
To further enhance the understanding and optimization of dextrin-related research, tools like PubCompare.ai's AI-powered platform can be invaluable.
These tools can help researchers identify the most effective dextrin products and procedures by comparing data from literature, pre-prints, and patents.
By simplifying the research process and providing access to the best reproducible protocols, PubCompare.ai can help streamline dextrin-related investigations and advance scientific knowledge in this field.
Whether you're working with dextrin, maltose dextrin, or other related carbohydrates, PubCompare.ai's AI-driven research reproducibility platform can be a valuable resource for your research endeavors.
Explore our dextrin comparison and optimization tools to locate the best protocols and simplify your work.