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Dextrose, Anhydrous

Dextrose, Anhydrous is a monosaccharide sugar and the dextrorotatory form of glucose.
It is a commonly used energy source and substrate in pharmaceutical and biochemical applications.
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Most cited protocols related to «Dextrose, Anhydrous»

1
Gestational Diabetes Screening Among Pregnant Women
Cited 26 times
We performed a cross‐sectional study with baseline data from the Pregnant+ study, a randomised controlled trial (RCT) among pregnant women with GDM [24 (link)] (ClinicalTrials.gov/NCT02588729). Data were collected from October 2015 to April
2017 at five diabetic outpatient clinics (DOCs) in the Oslo region of Norway. Participants were recruited consecutively as they came to the DOC. To be included in the study, the women had to have a smartphone, be 18 years or older and be at a gestational age of less than 33 weeks. The women also had to be capable of filling out the questionnaire in Norwegian, Somali or Urdu. Only 14 women filled out the questionnaire in either Urdu or Somali. Participants were excluded from the study if they had a twin pregnancy. In addition, women with celiac disease or lactose intolerance were excluded since they need to follow special diets [24 (link)]. Health professionals at the DOCs identified pregnant women with GDM and checked their eligibility for participation in the study. Of 774 participants assessed for eligibility, 238 participated in the study. All of the women were diagnosed with GDM after performing a two‐hour oral glucose tolerance test (OGTT) ≥ 9 mmol/L. The OGTT consisted of a fasting blood glucose sample followed by drinking a beverage containing 75 g of anhydrous glucose and a second blood glucose sample measured 2 hours later. The definition of GDM was in accordance with the national guidelines for antenatal care and that of the WHO [25 , 26 ].
Garnweidner-Holme L., Henriksen L., Bjerkan K., Lium J, & Lukasse M. (2022). Factors associated with the level of physical activity in a multi-ethnic pregnant population – a cross-sectional study at the time of diagnosis with gestational diabetes. BMC Pregnancy and Childbirth, 22, 1.
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Publication 2022
Beverages Blood Glucose Care, Prenatal Celiac Disease Dextrose, Anhydrous Diet Eligibility Determination Gestational Age Health Personnel Lactose Intolerance Oral Glucose Tolerance Test Pregnant Women Woman
2
National Diabetes Survey of Pakistan II
Cited 9 times
The second NDSP was conducted from February 2016 to August 2017 in all four provinces of Pakistan, that is, Punjab, Sindh, Khyber Pakhtunkhwa and Baluchistan.
Pakistani nationals aged 20 years or more were included in the survey, whereas pregnant women and those not residents of the selected households were excluded. An estimated sample size of 10 800 was calculated using probability sampling and multistage stratified sampling techniques.17 Sample size was calculated based on an expected prevalence of 18% (based on previous surveys), level of significance 97%, margin of error 1% with a design effect of 2, considering complex multistage design.
Stratification of population was done on the basis of urban and rural domains for all four provinces as defined in the latest available census.18 Each province was considered as a stratum and the districts (geographical subdivision of provinces legally described by government) considered as clusters were selected from each stratum. Tehsils or towns (further bifurcation of districts legally described by government) considered as subclusters were selected from each identified cluster for the survey.
Clusters and subclusters were randomly selected using probability proportional to size technique, and number of clusters were selected from each province using the ‘rule of thumb’ number of clusters (k)=(sample size of stratum/2)0.5.19 Twenty-seven clusters were selected out of a total 213 clusters from all over Pakistan. A total of 46 subclusters (21 from urban and 25 from rural) were selected (figure 1).
Seventeen teaching hospitals and/or diabetes centres participated in the second NDSP. The training sessions of these 17 teams were conducted from February 2016 to July 2016. The teams were trained to identify households, to fill the questionnaire, to take anthropometric and clinical measurements and to collect blood samples. The questionnaire was adopted from the WHO Questionnaire used in the 1st NDSP.8 (link) Each team was led by a physician as provincial coordinator of that cluster and each team comprised laboratory technicians, paramedical staff and survey officers.
Door-to-door assessment was done following systematic sampling technique. The first household in the lane was selected randomly and afterwards every 10th house was identified. In case residents of the identified household were not present or if they refused to participate, the next consecutive household was taken. Teams marked the houses and informed the adult residents. The selected household members were requested to come after an overnight fast (at least 8 hours) to the camp on the specific day. Two hundred and fourteen camps were conducted to recruit the required number of study subjects. Each participant was expected to stay within the screening facility for at least 2 hours, that is, for the post 75 g anhydrous glucose load. Meanwhile, the anthropometric and clinical data were collected by the trained paramedic staff under the supervision of the provincial coordinator.
Basit A., Fawwad A., Qureshi H, & Shera A.S. (2018). Prevalence of diabetes, pre-diabetes and associated risk factors: second National Diabetes Survey of Pakistan (NDSP), 2016–2017. BMJ Open, 8(8), e020961.
Publication 2018
Adult BLOOD Dextrose, Anhydrous Diabetes Mellitus Households Laboratory Technicians Paramedical Personnel Physicians Pregnant Women Supervision
3
Standardized Oral Glucose Tolerance Tests
Cited 5 times
Oral glucose tolerance tests followed concordant standardized operating procedures (SOP) in both studies [9] . Participants were asked to fast for ≥8 hours except for water (eg. from 22∶00 p.m.) and should not smoke or consume caffeine containing drinks, such as coffee and avoid sports before the examination. In SHIP-TREND and KORA F4, OGTTs were completed during morning hours. Fasting glucose was sampled, and 75 grams of anhydrous glucose (Dextro OGT; Boehringer Mannheim, Mannheim Germany) was given to participants without known diabetes (SHIP-TREND: known diabetes or current use of glucose-lowering agents; KORA F4: current use of glucose-lowering agents or unknown (newly study-diagnosed) diabetes at baseline (S4), validated by a physician). Fasting glucose and 2 h postload glucose were measured using a hexokinase method. In SHIP-TREND, Dimension Vista by Siemens Healthcare Diagnostics (Eschborn, Germany) and in KORA F4, GLU flex by Dade Behring (Marburg, Germany) were used.
Participants with fasting glucose values <5.6 mmol/l (<100 mg/dl) and 2 h glucose <7.8 mmol/l (<140 mg/dl) were defined as having normal glucose tolerance (NGT) (5). Fasting glucose values ≥7.0 mmol/l (≥126 mg/dl) or 2 h glucose ≥11.1 mmol/l (≥200 mg/dl) were classified as unknown diabetes. Prediabetes was diagnosed if fasting glucose values ranged between 5.6 and 6.9 mmol/l (100–125 mg/dl, i-IFG) and/or 2 h postload glucose values between 7.8 and 11.0 mmol/l (140–199 mg/dl, i-IGT). Participants were categorized to three groups of prediabetes (glucose disturbances): isolated impaired fasting glucose (i-IFG), isolated impaired glucose tolerance (i-IGT), and combined IFG and IGT (IFG+IGT).
Tamayo T., Schipf S., Meisinger C., Schunk M., Maier W., Herder C., Roden M., Nauck M., Peters A., Völzke H, & Rathmann W. (2014). Regional Differences of Undiagnosed Type 2 Diabetes and Prediabetes Prevalence Are Not Explained by Known Risk Factors. PLoS ONE, 9(11), e113154.
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Publication 2014
3,3'-diallyldiethylstilbestrol Caffeine Coffee Dextrose, Anhydrous Diabetes Mellitus Glucose Hexokinase Immune Tolerance Intolerances, Glucose Oral Glucose Tolerance Test Physicians Smoke States, Prediabetic
4
Hertfordshire Cohort Study: Comprehensive Assessments
Cited 5 times

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Publication 2014
Angina Pectoris Angioplasty Blood Pressure Bronchitis Cerebrovascular Accident Chest Pain Childbirth Coronary Artery Bypass Surgery Dextrose, Anhydrous Diabetes Mellitus Diagnosis Drugs, Non-Prescription Electrocardiography Ethics Committees, Research Fracture, Bone Health Visitors High Blood Pressures Husband Ilium Oral Glucose Tolerance Test Performance, Physical physiology Pregnancy Scapula Skinfold Thickness Transient Ischemic Attack Visiting Nurses Woman
5
Diabetes Ascertainment in the Women's Health Initiative
Cited 5 times
Outpatient and inpatient medical records related to diabetes diagnosis, treatment, and laboratory testing were requested for consenting women and reviewed by a trained physician adjudicator (KM, CL, SW, DB) at each site. Medical records of all self-reported incident and prevalent diabetes cases were requested and reviewed. The records of any participant from the No Diabetes Group who had only one fasting glucose value available, any fasting glucose value of >100 mg/dL, or who reported diabetes on the confirmation questionnaire were also requested and reviewed. If all of two or more fasting glucose values were <100 mg/dL and the participant reported no diabetes on the confirmation questionnaire, she was assumed without further review not to have diabetes.
The medical record review form included notations of diabetes on the problem list or as a diagnosis, the use of medications for diabetes (oral medications and/or insulin), glycosylated hemoglobin levels, fasting and casual plasma glucose values, and results of any oral glucose tolerance tests. Based on the medical records reviewed, the adjudicators recorded their summary impression about whether the participant had diabetes and listed the supporting diagnostic criteria on which their decision was based. Diabetes was defined at the study's inception in accordance with the American Diabetes Association definition[25 (link)] as one or more of the following found in the medical record: 1) Fasting plasma glucose >126 mg/dL, 2) Symptoms of diabetes (polyuria, polydipsia, and unexplained weight loss) plus casual plasma glucose concentration >200 mg/dL, 3) 2-hour postload glucose >200 mg/dL during an oral glucose tolerance test using a glucose load containing the equivalent of 75 g anhydrous glucose dissolved in water, and 4) Treatment with insulin or an oral hypoglycemic agent.
All physician adjudicators participated in a training session in which 12 sample charts were reviewed and discussed. Furthermore, shortly after medical record abstraction was under way, quality assurance checks were conducted; a second adjudicator (KM) independently reviewed 12 randomly selected records (5 incident, 5 prevalent, and 2 nondiabetes cases) from each site and verified the summary impression of diabetes vs. no diabetes, as supported by the diagnostic elements listed above. Agreement between adjudicators regarding diabetes status was 100%. In addition to the quality assurance sample, the medical records of any discordant cases (WHI self-report of diabetes did not match summary impression from medical record review) were reviewed by a second physician adjudicator (KM), who made a final determination of diabetes status. The WHI cohort was also linked to Medicare data from 1991 to 2007. Of women aged 65 and older with a valid Social Security number, 196 were successfully matched to the Medicare enrollment file.
Jackson J.M., DeFor T.A., Crain A.L., Kerby T.J., Strayer L.S., Lewis C.E., Whitlock E.P., Williams S.B., Vitolins M.Z., Rodabough R.J., Larson J.C., Habermann E.B, & Margolis K.L. (2014). Validity of Diabetes Self-Reports in the Women's Health Initiative. Menopause (New York, N.Y.), 21(8), 861-868.
Publication 2014
Dextrose, Anhydrous Diabetes Mellitus Diagnosis Glucose Hemoglobin, Glycosylated Hypoglycemic Agents Inpatient Insulin Oral Glucose Tolerance Test Outpatients Pharmaceutical Preparations Physicians Plasma Polydipsia Polyuria Woman

Most recents protocols related to «Dextrose, Anhydrous»

1
Carotid Ultrasound and Metabolic Testing
Color Doppler ultrasound (GE Healthcare, Milwaukee, Wisconsin) was used as the diagnostic method for CAD. Experienced sonographers were blinded to all clinical information performed in the evaluation. Carotid intima-media thickness (IMT) and carotid plaque were recorded during the ultrasound examination; IMT was defined as the distance between the leading edge of the first and second echo lines of the distant arterial wall; the presence of CAP was defined as a local increase in thickness of 0.5 mm or 50% of the surrounding IMT value (15 (link)).
Subjects underwent standard OGTT and IRT tests the following morning after fasting (8-10 hours). After fasting venous blood collection for determination of fasting glucose (OGTT0H) and fasting insulin (IRT0H), patients were instructed to take 75 g of anhydrous glucose within 5 minutes, and blood samples were collected from the anterior elbow vein at intervals of 30, 60, 120 and 180 minutes for determination of plasma glucose concentrations (OGTT0.5H, OGTT1H, OGTT2H, OGTT3H) and serum insulin concentrations (IRT0.5H, IRT1H, IRT2H, IRT3H).
Feng X., Ren L., Xiang Y, & Xu Y. (2023). Development and validation of a nomogram for evaluating the incident risk of carotid atherosclerosis in patients with type 2 diabetes. Frontiers in Endocrinology, 14, 1131430.
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Publication 2023
Arterial Lines BLOOD Blood Glucose Carotid Arteries Carotid Intima-Media Thickness Dental Plaque Dextrose, Anhydrous Diagnosis ECHO protocol Elbow Glucose Insulin Oral Glucose Tolerance Test Patients Plasma Serum Tunica Intima Ultrasonics Ultrasounds, Doppler Veins
2
Preparation of M63 Media with Toxic Compounds
M63 media (VWR Life Science) solution was made by first diluting 1 liter of presterilized M63 5× (BioWORLD, GeneLinx International Inc.) stock solution using autoclaved Millipore water. Filter-sterilized magnesium sulfate anhydrous (MgSO4, Fisher Scientific) water solution, of volume 1 mL and molarity of 1 M, was added to the diluted media solution following standard protocol. Sodium arsenate stock solution (RICCA Chemical Company, 100 mM) was first filter-sterilized and then diluted with sterilized DI water to reach concentrations of 0.1 mM and 0.1 µM and stored under 4 °C. Potassium dichromate (Fisher Scientific) solution was made by first dissolving sodium dichromate crystal into sterilized DI water to reach concentrations of 17 mM, and then, the solution was filter-sterilized and diluted with sterilized DI water again to reach concentrations of 0.34 mM and 0.34 µM and stored at 4 °C. Prior to exposure to bacterial cultures, working solutions were placed at room temperature for 30 min to equilibrate to ambient temperature and then titrated to the culture to target exposure concentration. Anhydrous dextrose (glucose, Fisher Scientific), 1 g, was dissolved in 10 mL DI water and filter-sterilized to form 10% (w/v) glucose stock solution, which was added into the media solution later to provide energy source for bacteria.
Wei H., Huang Y., Santiago P.J., Labachyan K.E., Ronaghi S., Banda Magana M.P., Huang Y.H., C. Jiang S., Hochbaum A.I, & Ragan R. (2023). Decoding the metabolic response of Escherichia coli for sensing trace heavy metals in water. Proceedings of the National Academy of Sciences of the United States of America, 120(7), e2210061120.
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Publication 2023
Bacteria Dextrose, Anhydrous Glucose Potassium Dichromate sodium arsenate sodium bichromate Sulfate, Magnesium
3
Phytochemical Characterization of Samples
The polysaccharide content was determined by the sulfuric acid-phenol method [39 (link)]. Briefly, the glucose standard curve was drawn with the mass concentration of D-anhydrous glucose as the abscissa (x) and the absorbance value as the ordinate (y). The linear regression equation of the standard curve was y = 0.0081x − 0.0039, R2 = 0.999. Referring to the extraction method established by Tian et al. [39 (link)], the sample treatment was slightly modified. Briefly, 1.0 g of each sample was accurately weighed, 100 mL of absolute ethanol was added and soaked overnight, filtered, and the filtrate was evaporated. The final volume of 50 mL was made with distilled water. The extract was shaken well and underwent ultrasonication (180 W, 40 kHz) for 40 min, reflux at 90 °C for 1 h, and centrifuge at 4000 r/min for 10 min. The supernatant was collected, and the absorbance was recorded at 490 nm after dilution.
The total coumarin content was determined by the spectrophotometry method, and the standard curve was drawn with osthole as the standard compound (y = 0.0549x − 0.0029; R2 = 0.9994). For each sample, 1.0 g of powder was accurately weighed, and 30 mL of anhydrous ethanol was added. The mixture was ultrasonicated (180 W, 40 kHz) for 30 min, heated and refluxed in a water bath for 30 min, and filtered. The absorbance was recorded at 320 nm after dilution.
The total polyphenols content was determined by the Folin phenol colorimetric method [40 (link)], and the standard curve was drawn with gallic acid as the reference standard (y = 3.7514x + 0.006, R2 = 0.9991). For each sample, 1.0 g of powder was accurately weighed, add 50 mL of 70% ethanol was added. The mixture was ultrasonicated (180 W, 40 kHz) for 30 min, heated to reflux in a water bath for 30 min, and filtered. The final volume was increased to 50 mL using 70% ethanol. The absorbance was recorded at 760 nm.
The total flavonoid content was determined by the aluminum chloride colorimetric method [40 (link)], and the standard curve was drawn with rutin as the reference substance (y = 10.39x + 0.0014, R2 = 0.9998). Briefly, the sample powder was accurately weighed (1.0 g), and 30 mL of 85% ethanol was added. The mixture was ultrasonicated (180 W, 40 kHz) for 30 min and heated under reflux in a water bath for 30 min. The filtered extract was made up to 50 mL with 85% ethanol. The absorbance was measured at a wavelength of 510 nm after dilution.
Tang W., Chen Y, & Guo F. (2023). Comparative Analysis of Roots from Vicatia thibetica de Boiss and Angelica sinensis Based on Chemical Composition, Antioxidant, Nitrite-Scavenging and Enzyme Inhibition Activities. Molecules, 28(4), 1942.
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Publication 2023
Absolute Alcohol Aluminum Chloride Bath Colorimetry coumarin Dextrose, Anhydrous Ethanol Flavonoids folin Gallic Acid Glucose osthol Phenol Polyphenols Polysaccharides Powder Rutin Specimen Handling Spectrophotometry Sulfuric Acids Technique, Dilution
4
Submerged Cultivation and Extraction of G. lucidum
The G. lucidum strain 5.1 from the collection of the Laboratory of Biosynthesis of Biologically Active Substances, Gauze Institute of New Antibiotics was used in this study. This strain was used in phylogenetic studies and the studied RNA sequence was deposited in the GenBank. Stock cultures were grown on potato glucose agar (PGA) at 26 °C for 7 days and then stored at 4 °C.
The liquid seed culture of G. lucidum was grown in 750 mL Erlenmeyer flasks containing 100 mL of liquid nutrient medium at 26 °C for 6 days. Unhopped beer wort (4° according to Balling scale) and pH 6.0 before sterilization was used as a liquid seed medium. The seed liquid medium was inoculated with mycelial agar plugs (3 mm diam.) of a seven-day culture of basidiomycete on PGA at the rate of 1/4 tube per flask.
Submerged cultivation of G. lucidum was carried out on a rotary shaker at 220 rpm and at 26 °C in 750 mL Erlenmeyer flasks containing 100 mL of nutrient medium inoculated with 10 mL of liquid seed culture for 7 days. The liquid nutrient medium for submerged cultivation was contained (g/l of water): anhydrous glucose 20.0, yeast extract (Serva) 10.0, potassium dihydrogen phosphate 2.0, and magnesium sulfate 0.2. All ingredients of the culture medium used were water-soluble, which ensured that no non-fungal compounds presence in the target films.
All nutrient media used in this work were sterilized at 1.2 atm for 30 min. The microbiological purity of cultures at all stages of work was controlled by using a light microscope BX41 (Olympus, Tokyo, Japan). The morphological characteristics of the submerged mycelium of the G. lucidum were observed by SEM using a high-resolution scanning electron microscope Mira II (Tescan, Warrendale, PA, USA).
After cultivation, the G. lucidum submerged mycelium was separated from the culture liquid by filtration, washed twice with distilled water, and lyophilized (Figure 2). The dry mycelium was ground with a T 25 ULTRA-TURRAX digital homogenizer (IKA, Staufen, Germany) at 10,000 rpm for 5 min in 48% aqueous ethanol. The ratio of mycelium to aqueous ethanol was 12.5 mg/mL. As a result, crushed mycelium of the G. lucidum in a solvent system (MEGl) was obtained. After that, the resulting suspension was centrifuged at 3500 rpm for 5 min and the supernatant was pipetted. As a result, the supernatant of a water/ethanol extract of the G. lucidum mycelium extract (EGl) was obtained.
Smirnov A., Anisimkin V., Krasnopolskaya L., Guliy O., Sinev I., Simakov V., Golyshkin A., Almyasheva N., Ageykin N, & Kuznetsova I. (2023). Features of the Formation of Sensitive Films Based on Mycelium of Higher Fungi for Surface and Plate Acoustic Waves Gas Sensors. Sensors (Basel, Switzerland), 23(4), 2216.
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Publication 2023
Agar Anabolism Antibiotics, Antitubercular Basidiomycota Beer Culture Media Dextrose, Anhydrous Ethanol Filtration Glucose Light Microscopy Mycelium Nutrients potassium phosphate, monobasic Potato RNA Sequence Scanning Electron Microscopy Solvents Strains Sulfate, Magnesium Yeast, Dried
5
Hfx. mediterranei Cd(II) Biosorption Protocol
For all the dilutions prepared, high-purity water (resistivity > 18 MΩ) from a Direct-Q 3V water purification system (Millipore Inc., Paris, France) was used.
For the incubation of Hfx. mediterranei, saltwater was prepared using CaCl2·H2O (Labken, Barcelona, Spain), KCl, NaBr, NaCl and NaHCO3 (Panreac, Castellar del Valles, Spain), and MgCl2·6H2O and MgSO4·7H2O (VWR Chemicals, Radnor, PA, USA). Two different incubation media were prepared from the saltwater: CM by adding yeast extract (Condalab, Madrid, Spain) and DM by adding NH4Cl, D(±)-Anhydrous glucose, Na2HPO4, NaH2PO4 (Panreac, Castellar del Valles, Spain), and Tris(hydroxymethyl)-aminomethane and FeCl3 from (Merck, Darmstadt, Germany). Cd (II) stock solution was prepared using the salt CdCl2·2.5 H2O (Merck, Darmstadt, Germany), and the pH of the medium was adjusted using HCl (Merck, Darmstadt, Germany) and NaOH (Panreac, Castellar del Valles, Spain).
Finally, for ICP analysis, sample digestion was conducted using 69% HNO3 (Panreac, Castellar del Valles, Spain) and the calibration standard solutions were prepared from the ICP-IV multi-elemental standard as well as Mo, Sc and Ru monoelemental standards, all reagents and purchased from Merck (Darmstadt, Germany).
Saez-Zamacona I., Grindlay G, & Martínez-Espinosa R.M. (2023). Evaluation of Haloferax mediterranei Strain R4 Capabilities for Cadmium Removal from Brines. Marine Drugs, 21(2), 72.
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Publication 2023
Bicarbonate, Sodium Chloride, Cadmium Dextrose, Anhydrous Digestion Magnesium Chloride methylamine Saccharomyces cerevisiae SALL2 protein, human Sodium Chloride Sulfate, Magnesium Technique, Dilution Tromethamine

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2
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More about "Dextrose, Anhydrous"

Dextrose, Anhydrous is a monosaccharide sugar and the dextrorotatory form of glucose, also known as alpha-D-Glucose or simply Anhydrous glucose.
It is a commonly used energy source and substrate in pharmaceutical and biochemical applications, including as a component in various solutions, buffers, and growth media.
Dextrose, Anhydrous is a reducing sugar that can be utilized by cells as a fuel source through glycolysis and the citric acid cycle.
It is an important ingredient in intravenous (IV) fluids and parenteral nutrition formulations, providing calories and electrolytes to support hydration and metabolic needs.
In addition to its medical and biological applications, Dextrose, Anhydrous has various other uses.
It can be employed as a sweetener, preservative, and texturizing agent in food and beverage products.
The anhydrous form is preferred in some cases over regular glucose due to its higher purity and stability.
Researchers studying Dextrose, Anhydrous can leverage the power of AI-driven comparison platforms like PubCompare.ai to optimize their work.
These tools can help locate relevant protocols from literature, preprints, and patents, and provide intelligent comparisons to identify the best products and methodologies for their needs.
By utilizing the insights and capabilities of AI, researchers can elevate their Dextrose, Anhydrous-related research and discover the optimal solutions for their projects.
Other related terms and subtopics to consider include Acetonitrile, which is sometimes used in the analysis of Dextrose, Anhydrous; the Selectra 2 auto-analyzer, which can be used to measure Dextrose levels; and compounds like Gallic acid, Rutin, and Formic acid, which may be used in conjunction with Dextrose, Anhydrous in certain applications.
Ethanol is also a common solvent used in experiments and procedures involving Dextrose, Anhydrous.