The ginger extract (50 g) was chromatographed on a Sephadex LH-20 column with 95% ethanol as eluant to remove the nonphenolic compounds (Fraction 1, 21.8 g) and to generate the gingerols and shogaols enriched fraction (Fraction 2, 28 g). Fraction 2 was then loaded into a Diaion HP-20 column eluted first with water to remove the water soluble compounds and then with 40% aqueous ethanol to obtain fraction A (9 g) followed by 95% aqueous ethanol to obtain fraction B (11g). Fraction A (5g) was subjected to a normal phase silica gel column with a stepwise gradient of hexane/ethyl acetate [9:1; 8:2, and 7:3] to give pure [6]- (2g), [8]- (0.5 g), and [10]-gingerol (0.4 g). Fraction B (5 g) was also subjected to a normal phase silica gel column with a stepwise gradient of hexane/ethyl acetate [9:1 and 8:2] to generate 13 fractions. Fraction B5 (1g) was subjected to a C-18 reverse phase column eluted with a stepwise gradient of methanol/water [3:2, 7:3, and 4:1] to give [6]-paradol (40 mg), [1]-dehydrogigerdione (60 mg), and [10]-shogaol (120 mg). Following a similar procedure, fraction B7 (1.5 g) gave 200 mg [8]-shogaol and 1 g of [6]-shogaol. The purification procedure was guided by TLC and HPLC analysis. The structures of these eight compounds were confirmed based on their 1H and 13C NMR analysis (Figure 1 ).
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Diaion HP 20
Diaion HP 20
Diaion HP 20 is a synthetic adsorbent resin that has been widely used in various applications, including pharmaceutical, chemical, and environmental industries.
It is a macroporous, non-ionic polymeric adsorbent with a high surface area, making it effective for the adsorption and separation of a wide range of organic compounds.
Diaion HP 20 can be utilized in protocols found in the literature, preprints, and patents to optimize research processes and enhance reproducibility and accuracy.
By leveraging AI-driven comparisons, researchers can identify the best protocols and products for their specific needs, improving the efficacy and efficiency of their studies.
This versatile adsorbent resin continues to be an invaluable tool in the pursuit of scientific discovery and innovation.
It is a macroporous, non-ionic polymeric adsorbent with a high surface area, making it effective for the adsorption and separation of a wide range of organic compounds.
Diaion HP 20 can be utilized in protocols found in the literature, preprints, and patents to optimize research processes and enhance reproducibility and accuracy.
By leveraging AI-driven comparisons, researchers can identify the best protocols and products for their specific needs, improving the efficacy and efficiency of their studies.
This versatile adsorbent resin continues to be an invaluable tool in the pursuit of scientific discovery and innovation.
Most cited protocols related to «Diaion HP 20»
Carbon-13 Magnetic Resonance Spectroscopy
Diaion HP 20
Ethanol
ethyl acetate
ginger extract
gingerol
Hexanes
High-Performance Liquid Chromatographies
Methanol
sephadex LH 20
shogaol
Silica Gel
Equal size agar plugs with the mycelium of the pure FT1307 fungus grown on PDA media for five days was aseptically transferred to liquid broth media-containing 1-L conical flasks (350 mL × 65 for the large scale; 350 mL × 3 for the small scale). The liquid broth media consisted of mannitol 20 g, sucrose 10 g, monosodium glutamate 5 g, KH2PO4 0.5 g, MgSO4·7H2O 0.3 g and 3 g of yeast extract dissolved in 1 L of distilled water (pH of the liquid media adjusted to 6.5 before sterilization). Then, the media flasks were sterilized for 15 min at 121 °C. The inoculated flasks were fermented for 28 days at 24 °C under static conditions. After the completion of the fermentation period, extraction was carried out as described in our previous publications [18 (link),19 (link)] with slight modifications. Aqueous mycelium and broth media treated with Diaion HP-20 resins were loaded into an open column and separated using different concentrations of MeOH/H2O systems (10, 50, 90 and 100% MeOH) as the eluent. The 90% and 100% fractions shared a similar RP-HPLC profile. Hence, both fractions were combined (3.5 g) and subjected to further purification.
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Agar
Diaion HP 20
Fermentation
High-Performance Liquid Chromatographies
Mannitol
Mycelium
Resins, Plant
Sodium Glutamate
Sucrose
Sulfate, Magnesium
Yeasts
Dried slices of roots of PM (2 kg) were pulverized and soaked in 60% methanol (10 L) for one day. After being filtered, the residues were twice extracted with 60% methanol (10 L). The filtrate was combined and concentrated under a vacuum with a rotary evaporator to obtain an aqueous solution. The aqueous solution was chromatographed on a Diaion HP-20 column (10 cm i.d × 45 cm, Mitsubishi Chemical Industry, Tokyo, Japan) eluted with H2O, 50% MeOH, and 100% MeOH (each 5 L). The 50% MeOH eluate (94.8 g) of the Diaion HP-20 column was chromatographed over a Sephadex LH-20 column (5.0 cm i.d. × 45 cm, GE Healthcare Biosciences, Uppsala, Sweden) eluted with MeOH (20 mL/1.5 min/tube) to give a THSG-enriched fraction (tube no. 53-69). The THSG-enriched fraction (5 g) dissolved in water was chromatographed on a LiChroprep RP-18 column (2.5 cm i.d. × 43.5 cm, Merck, Darmstadt, Germany) eluted with 0.05% trifluoroacetic acid–CH3CN (82:18) to give an amorphous powder of THSG (2.3 g). The purity of THSG was 98.7% determined by HPLC (Waters 1525 binary pump connected with 717 plus autosampler and 2487 dual λ absorbance detector, Waters, Milford, MA, USA). A flowchart of THSG isolation is shown in Figure 2 . The structure of THSG (Figure 1 ) was identified by comparison of its 1H and 13C nuclear magnetic resonance (NMR), (Bruker Avance DRX 500MHz, Bruker Corp., Billerica, MA, USA) data with those reported in the literature [16 (link)].
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Diaion HP 20
High-Performance Liquid Chromatographies
isolation
Magnetic Resonance Imaging
Methanol
Plant Roots
Powder
sephadex LH 20
Trifluoroacetic Acid
Vacuum
Korean red ginseng extract, a water extract obtained from steamed and dried roots of P. ginseng, was employed for the isolation and purification of individual ginsenosides. Red ginseng water extract (1 kg) was dissolved in water to make approximately 20% to 25% (w/v) solution. The solution was passed through a Diaion HP-20 adsorption column (8 L), and the resin was washed with 4 wet resin volumes of 20% ethanol. Crude saponin adsorbed to the macro-reticular resin was eluted with 4 wet resin volumes of pure methanol. The methanol elute was then dried with silica gel powder in vacuo to obtain a pale brown powder. The powder was loaded on a glass column (15×100 cm) containing 3.5 L of silica gel and partitioned with solvent mixtures of CHCl3-MeOH-H2O (12:3:1, 9:3:1, 7:3:1, 4:3:1; lower phase, 4 bed volumes for each mixture). Fractions containing the ginsenosides Rg3 were dried in vacuo and recrystallized in water until a white powder was formed. The white crystalline amorphous powder was then subjected to preparative HPLC ( 20×250 mm, ODS YMC-Pack, 203 nm) with aqueous 45% CH3CN as the mobile phase to purify single ginsenoside. The peak corresponding to each ginsenoside was collected and evaporated to dryness and further dried in a vacuum drier for 5 d. The resultant white powder was stored at -20℃ until further use.
Adsorption
Chloroform
Diaion HP 20
Ethanol
Ginseng
Ginsenoside
ginsenoside Rg3
Ginsenosides
High-Performance Liquid Chromatographies
isolation
Korean Red Ginseng
Methanol
Plant Roots
Powder
Resins, Plant
Saponin
Silica Gel
Solvents
Vacuum
Fresh ripe cherry tomato fruit (Lycopersicon esculentum var. cerasiforme) were crushed, added with water, and centrifuged. The supernatant was passed through a Diaion® HP-20 (Mitsubishi Chemical, Tokyo, Japan) and separated by gradient elution with 40% aq. methanol, 60% aq. methanol, and methanol, successively. The 60% eluate provided EsA. EsA was checked with thin-layer chromatography, and the average EsA yield was calculated as about 0.043%. EsA was hydrolyzed with 2 N HCl, and then the reaction mixture was extracted with ethyl acetate. The organic layer was evaporated in vacuum to afford a residue, which was purified by silica gel column chromatography with CHCl3-methanol-H2O = 9:1:0.1 to give Esg-A [2 (link),14 (link)]. The chemical structure of EsA is shown in Figure 1 .
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Chloroform
Chromatography
Diaion HP 20
ethyl acetate
Fruit
Gel Chromatography
Lycopersicon esculentum
Methanol
Prunus cerasus
Silica Gel
Silicon Dioxide
Thin Layer Chromatography
Vacuum
Most recents protocols related to «Diaion HP 20»
The dried DS were decocted twice with eightfold and tenfold distilled water at 100°C, respectively (1 h each time). Supernatants were combined, concentrated, and dried under a vacuum to obtain an aqueous extract of DS (extract ratio: 7.26%), which was extracted with petroleum ether to obtain DS-FO. Then, the residue was concentrated and eluted with water, 20% ethanol (v/v), and 80% ethanol (v/v) on a column (Diaion HP-20; MilliporeSigma) to obtain the water-elution fraction, 20% ethanol fraction (corresponding to DS-FG), 80% ethanol fraction (corresponding to DS-FA), respectively. Subsequently, the water-elution fraction was precipitated with 95% ethanol (v/v) to obtain the precipitate (DS-Pol) and supernatant (DS-Oli). All fractions were stored at 4°C. The extract ratio of the five fractions (DS-Pol/DS-Oli/DS-FG/DS-FA/DS-FO) were: 1.231%, 1.306%, 0.289%, 0.517%, 28.8%. DS and its fractions were isolated systematically, purified and identified accurately in the previous phytochemical studies described by Feng and his colleagues (Gong et al., 2014 (link); Feng et al., 2015 (link); Feng et al., 2016 (link)). The chemical profiles of DS and its fractions were characterized by our team through HPLC combined with three detectors (PDA/ELSD/UV) and LC-MS analysis to guarantee stability and reproducibility of the fraction-splitting method and non-overlapping property of different fractions (Zhang, 2015 (link); Zhou et al., 2017 (link)).
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Diaion HP 20
Ethanol
High-Performance Liquid Chromatographies
naphtha
Phytochemicals
Vacuum
The following instruments were used to obtain spectroscopic data: specific rotation, JASCO P-2200 polarimeter (JASCO Corporation, Tokyo, Japan, l = 5 cm); UV spectra, Shimadzu UV-1600 spectrometer; IR spectra, IRAffinity-1 spectrophotometer (Shimadzu, Kyoto, Japan); 1H NMR spectra, JNM-ECA800 (800 MHz), JNM-LA500 (500 MHz), JNM-ECS400 (400 MHz), and JNM-AL400 (400 MHz) spectrometers; 13C NMR spectra, JNM-ECA800 (200 MHz), JNM-LA500 (125 MHz), JNM-ECA400 (100 MHz), and JNM-AL400 (100 MHz) spectrometers (JEOL, Tokyo, Japan); EI-MS and high-resolution EI-MS, JMS-GCMATE mass spectrometer (JEOL, Tokyo, Japan); HPLC detector, SPD-10Avp UV-VIS detector; and HPLC columns, Cosmosil 5C18-MS-II (Nacalai Tesque, Kyoto, Japan). For NMR, the samples were dissolved in deuterated chloroform (CDCl3) at room temperature with tetramethylsilane as an internal standard. Columns of 4.6 mm × 250 mm and 20 mm × 250 mm were used for analytical and preparative purposes, respectively.
The following chromatographic materials were used for column chromatography (CC): highly porous synthetic resin, Diaion HP-20 (Mitsubishi Chemical, Tokyo, Japan); normal-phase silica gel CC, silica gel 60 N (Kanto Chemical, Tokyo, Japan; 63–210 mesh, spherical, neutral); reversed-phase ODS CC, Chromatorex ODS DM1020T (Fuji Silysia Chemical, Aichi, Japan; 100–200 mesh); TLC, pre-coated TLC plates with silica gel 60F254 (Merck, Darmstadt, Germany, 0.25 mm) (normal-phase) and silica gel RP-18 WF254S (Merck, 0.25 mm) (reversed-phase); and reversed-phase HPTLC, pre-coated TLC plates with silica gel RP-18 WF254S (Merck, 0.25 mm). Detection was performed by spraying with 1% Ce(SO4)2–10% aqueous H2SO4, followed by heating.
The following chromatographic materials were used for column chromatography (CC): highly porous synthetic resin, Diaion HP-20 (Mitsubishi Chemical, Tokyo, Japan); normal-phase silica gel CC, silica gel 60 N (Kanto Chemical, Tokyo, Japan; 63–210 mesh, spherical, neutral); reversed-phase ODS CC, Chromatorex ODS DM1020T (Fuji Silysia Chemical, Aichi, Japan; 100–200 mesh); TLC, pre-coated TLC plates with silica gel 60F254 (Merck, Darmstadt, Germany, 0.25 mm) (normal-phase) and silica gel RP-18 WF254S (Merck, 0.25 mm) (reversed-phase); and reversed-phase HPTLC, pre-coated TLC plates with silica gel RP-18 WF254S (Merck, 0.25 mm). Detection was performed by spraying with 1% Ce(SO4)2–10% aqueous H2SO4, followed by heating.
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1H NMR
Carbon-13 Magnetic Resonance Spectroscopy
Chloroform
Chromatography
Chromatography, Reverse-Phase
Diaion HP 20
Gel Chromatography
High-Performance Liquid Chromatographies
Infrared Spectrophotometry
Resins, Synthetic
Silica Gel
Silicon Dioxide
Spectrum Analysis
tetramethylsilane
The preparation of EFE was carried out as described previously [10 (link)]. Briefly, Ephedra Herb (E. sinica, the Japanese pharmacopoeia grade) was added to water, extracted at 95 °C for 1 h, and filtered, after which the residue was washed with water. The extract was centrifuged at 1800× g for 10 min, and then the supernatant was passed directly through the DIAION SK-1B ion-exchange resin. The unadsorbed fraction was adjusted to pH 5 using 5% NaHCO3 aq., and the solution was then evaporated under reduced pressure to obtain EFE.
EMCT was prepared according to previous reports [8 (link)]. Briefly, Ephedra Herb extract was dissolved in H2O and extracted with ethyl acetate and n-butanol. The H2O extract obtained was separated using column chromatography over DIAION HP-20 with methanol (MeOH)-H2O (0:100→100:0) in stepwise grade mode. Then, 40% MeOH eluate was separated using column chromatography on Sephadex LH-20 with MeOH-H2O (50:50→80:20) and 70% acetone in a stepwise-gradient manner to obtain a 70% acetone fraction as the EMCT fraction. The confirmation of EMCT was performed by gel permeation chromatography [8 (link)]. EFE (10 mg/mL) and EMCT (1 mg/mL) were dissolved in ultra-pure water and stored at –80 °C until further use.
EMCT was prepared according to previous reports [8 (link)]. Briefly, Ephedra Herb extract was dissolved in H2O and extracted with ethyl acetate and n-butanol. The H2O extract obtained was separated using column chromatography over DIAION HP-20 with methanol (MeOH)-H2O (0:100→100:0) in stepwise grade mode. Then, 40% MeOH eluate was separated using column chromatography on Sephadex LH-20 with MeOH-H2O (50:50→80:20) and 70% acetone in a stepwise-gradient manner to obtain a 70% acetone fraction as the EMCT fraction. The confirmation of EMCT was performed by gel permeation chromatography [8 (link)]. EFE (10 mg/mL) and EMCT (1 mg/mL) were dissolved in ultra-pure water and stored at –80 °C until further use.
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Acetone
Bicarbonate, Sodium
Butyl Alcohol
Chromatography
Diaion HP 20
Ephedra
ethyl acetate
Gel Chromatography
Ion Exchange Resins
Japanese
Methanol
Pressure
sephadex LH 20
The methanolic extract (40 g) was subjected to open column chromatography (column dimensions 100 × 1000 mm), with Diaion HP 20 as stationary phase, starting with 100% water as an eluting solvent followed by water:MeOH (6:4), gradually changing to 100% MeOH. All the fractions were dried under reduced pressure. This resulted in the following subfractions: 100% H2O (MA), MeOH 40% (MB), MeOH 50% (MC), MeOH 60% (MD), MeOH 70% (ME), MeOH 80% (MF), MeOH 90% (MG), and MeOH 100% (MH). The subfractions MB, MC, ME, MF, and MG were submitted to UPLC-HRMS. The subfractions MA and MD were not submitted to UPLC-HRMS, since those subfractions did not reveal any spots after NP-TLC analysis. The subfractions MF and MG were combined because they showed a similar pattern after NP-TLC analysis.
The subfraction MF/MG (2.3653 mg) was submitted to flash chromatography on a Reveleris C18 cartridge by solid injection. The mobile phase used was H2O + 0.1% formic acid (A) and acetonitrile + 0.1% formic acid (B), with the following gradient: 0 min 95% for A and 5% of B retained for 15 min, which then changed to 50% for A and 50% for B at 52 min; from 97–111 min, a linear change to 0% for A and 100% for B, and from 111 min, a linear change to 95% for A and 5% for B, with a flow rate of 13 mL/min. The eluent was collected according to the signals measured by the evaporative light scattering detector (ELSD) and ultraviolet (UV) absorption at 210 and 254 nm; NP-TLC analysis was performed with n-BuOH-AcOH-H2O 4-1-5 as the mobile phase. The subfractions of FG showing a similar pattern were combined, resulting in 15 subfractions.
Isolation was conducted on a semi-preparative HPLC-DAD-MS system (Waters, Millford, MA, USA) with Masslynx™software version 4.1. A Phenomenex Luna C18(2) 100 Å; 250 × 10.00 mm, 5 μm column was used together with a pre-column. The subfraction MFMG.9 (101.9 mg) was further purified by semi-preparative HPLC-DAD-MS with a C18 Luna column and the mobile phase H2O + 0.1% formic acid (A) and acetonitrile + 0.1% formic acid (B), and the following gradient: 0 to 5 min 42% of B, 30 min 50% of B, 35–40 min 100% of B, and 45–55 42% of B. The flow rate was 4.75 mL/min. The mass spectrometer was operated in ESI+ mode, with an MS scan range of m/z 250 to 800; Vcapillary 3.5 kV; Vcone 50 V; Vextractor 3V; VRF Lens 0.2 V; Tsource 125 °C; Tdesolvation 400 °C, and a desolvation gas flow of 750 L/h and a cone gas flow of 0 L/h to collect a compound with m/z 485.2 and m/z 763.3 (compound1 ).
The subfraction MFMG.8 (175 mg) was submitted to the semi-preparative HPLC-DAD-MS with the following gradient: 0 to 5 min 35% of B, 10 min 50% of B, 35–40 min 100% of B, and 45–55 32% of B, to collect a compound with m/z 485.2 and m/z 763.3 (compound2 ).
The subfraction MF/MG (2.3653 mg) was submitted to flash chromatography on a Reveleris C18 cartridge by solid injection. The mobile phase used was H2O + 0.1% formic acid (A) and acetonitrile + 0.1% formic acid (B), with the following gradient: 0 min 95% for A and 5% of B retained for 15 min, which then changed to 50% for A and 50% for B at 52 min; from 97–111 min, a linear change to 0% for A and 100% for B, and from 111 min, a linear change to 95% for A and 5% for B, with a flow rate of 13 mL/min. The eluent was collected according to the signals measured by the evaporative light scattering detector (ELSD) and ultraviolet (UV) absorption at 210 and 254 nm; NP-TLC analysis was performed with n-BuOH-AcOH-H2O 4-1-5 as the mobile phase. The subfractions of FG showing a similar pattern were combined, resulting in 15 subfractions.
Isolation was conducted on a semi-preparative HPLC-DAD-MS system (Waters, Millford, MA, USA) with Masslynx™software version 4.1. A Phenomenex Luna C18(2) 100 Å; 250 × 10.00 mm, 5 μm column was used together with a pre-column. The subfraction MFMG.9 (101.9 mg) was further purified by semi-preparative HPLC-DAD-MS with a C18 Luna column and the mobile phase H2O + 0.1% formic acid (A) and acetonitrile + 0.1% formic acid (B), and the following gradient: 0 to 5 min 42% of B, 30 min 50% of B, 35–40 min 100% of B, and 45–55 42% of B. The flow rate was 4.75 mL/min. The mass spectrometer was operated in ESI+ mode, with an MS scan range of m/z 250 to 800; Vcapillary 3.5 kV; Vcone 50 V; Vextractor 3V; VRF Lens 0.2 V; Tsource 125 °C; Tdesolvation 400 °C, and a desolvation gas flow of 750 L/h and a cone gas flow of 0 L/h to collect a compound with m/z 485.2 and m/z 763.3 (compound
The subfraction MFMG.8 (175 mg) was submitted to the semi-preparative HPLC-DAD-MS with the following gradient: 0 to 5 min 35% of B, 10 min 50% of B, 35–40 min 100% of B, and 45–55 32% of B, to collect a compound with m/z 485.2 and m/z 763.3 (compound
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A-485 compound
acetonitrile
Chromatography
Diaion HP 20
Exanthema
formic acid
High-Performance Liquid Chromatographies
isolation
Lens, Crystalline
Light
Methanol
Pressure
Radionuclide Imaging
Retinal Cone
Solvents
The powdered aerial parts of H. rochelii (22.0506 g) were, subsequently, extracted with dichloromethane (CH2Cl2) (12 × 100 mL), MeOH (4 × 150 mL) and 80% aq. MeOH (2×100 mL). The resulting extracts were evaporated to dryness using a vacuum rotary evaporator. The CH2Cl2 extract gave a dark green waxy residue of 1.403 g (RochD). The MeOH and aq. MeOH residues were combined to give 4.4453 g of a brown semisolid (RochM).
The powdered aerial parts of H. hirsutum (412.0457 g) were extracted with CH2Cl2 (36 × 500 mL) at room temperature. The CH2Cl2 extract gave a dark green waxy residue of 40.8 g. The CH2Cl2 extract was subjected to column chromatography (CC) over a Diaion HP-20 (5 × 15 mm) and was, subsequently, eluted with 90% aq. MeOH (15 × 500 mL) and MeOH (5 × 500 mL) to obtain 2 pooled fractions of 24.1 g (HirDM90) and 5.1 g (HirDM100), respectively. An elution with CH2Cl2 gave a fraction containing lipids, chlorophyll and waxes (HirDD).
Powdered aerial parts of H. barbatum (12.102 g), H. rumeliacum (Bela voda, Pernik) (10.4469 g) and H. rumeliacum (Konyavska mountain) (11.4158 g) were extracted separately with CH2Cl2 (10 × 200 mL), combined and then evaporated in vacuo to give dark green waxy residues of 863.5 mg (BarbD), 539.4 mg (RumDBe) and 582 mg (RumDKo), respectively.
The powdered aerial parts of H. hirsutum (412.0457 g) were extracted with CH2Cl2 (36 × 500 mL) at room temperature. The CH2Cl2 extract gave a dark green waxy residue of 40.8 g. The CH2Cl2 extract was subjected to column chromatography (CC) over a Diaion HP-20 (5 × 15 mm) and was, subsequently, eluted with 90% aq. MeOH (15 × 500 mL) and MeOH (5 × 500 mL) to obtain 2 pooled fractions of 24.1 g (HirDM90) and 5.1 g (HirDM100), respectively. An elution with CH2Cl2 gave a fraction containing lipids, chlorophyll and waxes (HirDD).
Powdered aerial parts of H. barbatum (12.102 g), H. rumeliacum (Bela voda, Pernik) (10.4469 g) and H. rumeliacum (Konyavska mountain) (11.4158 g) were extracted separately with CH2Cl2 (10 × 200 mL), combined and then evaporated in vacuo to give dark green waxy residues of 863.5 mg (BarbD), 539.4 mg (RumDBe) and 582 mg (RumDKo), respectively.
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Chlorophyll
Chromatography
Diaion HP 20
Green S
Lipids
Methylene Chloride
pyranine
Vacuum
Waxes
Top products related to «Diaion HP 20»
Sourced in Japan, United States, Germany, Sweden
Diaion HP-20 is a polymeric adsorbent resin. It is a non-ionic, macroporous resin with high surface area and excellent adsorption properties. Diaion HP-20 is primarily used for the isolation and purification of various organic compounds.
Sourced in United States, Germany, Sweden
Diaion HP-20 is a porous, non-ionic, highly cross-linked polystyrene-divinylbenzene resin. It is a type of adsorption resin used for a variety of applications, such as the purification and isolation of organic compounds, the removal of impurities, and the fractionation of mixtures.
Sourced in Sweden, Japan, China, United States, Germany, United Kingdom, France, Italy, Canada
Sephadex LH-20 is a size-exclusion chromatography media developed by GE Healthcare for the separation and purification of a wide range of organic molecules, including proteins, peptides, nucleic acids, and small organic compounds. It is composed of cross-linked dextran beads and is designed for use in gravity-flow or low-pressure liquid chromatography applications.
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Diaion HP-20 is a non-ionic, macroporous, styrene-divinylbenzene copolymer resin. It is used for adsorption and separation of various organic compounds in liquid chromatography applications.
Sourced in United States, Germany, Japan, China, Chile, Sweden, United Kingdom, Ireland, India, Switzerland
Sephadex LH-20 is a gel filtration medium used for the separation and purification of molecules based on their size and molecular weight. It is a cross-linked dextran polymer that can be used in a variety of applications, including the separation of proteins, peptides, nucleic acids, and other biomolecules. The porous structure of the Sephadex LH-20 beads allows for the efficient separation of molecules, making it a versatile tool for researchers and laboratory professionals.
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RP-18 F254S is a type of reversed-phase silica gel thin-layer chromatography (TLC) plate. It is used for the separation, identification, and quantification of various organic compounds. The plate is coated with a layer of silica gel modified with n-octadecylsilyl (C18) functional groups, which provide a non-polar stationary phase. The plate also contains a fluorescent indicator (F254S) that allows for the visualization of separated compounds under ultraviolet (UV) light.
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Silica gel 60 is a porous, amorphous form of silicon dioxide commonly used as a stationary phase in column chromatography. It has a high surface area and is effective at adsorbing a wide range of organic and inorganic compounds. Silica gel 60 is available in various particle sizes and pore sizes to suit different chromatographic applications.
More about "Diaion HP 20"
Diaion HP 20 is a highly versatile synthetic adsorbent resin that has found widespread applications in the pharmaceutical, chemical, and environmental industries.
This macroporous, non-ionic polymeric adsorbent boasts a large surface area, making it highly effective for the adsorption and separation of a wide range of organic compounds.
Researchers can leverage protocols found in literature, preprints, and patents to optimize their research processes and enhance reproducibility and accuracy when using Diaion HP 20.
By utilizing AI-driven comparisons, scientists can identify the best protocols and products for their specific needs, improving the efficacy and efficiency of their studies.
Diaion HP-20, a similar adsorbent resin, has also been extensively used in various applications, including the purification and separation of natural products, such as those found in Sephadex LH-20.
Additionally, RP-18 F254S and Silica gel 60 are other adsorbents that can be used in conjunction with or as alternatives to Diaion HP 20, depending on the specific requirements of the research project.
This versitale adsorbent resin continues to be an invaluable tool in the pursuit of scientific discovery and innovation, enabling researchers to optimize their workflows and enhance the quality and reliability of their results.
This macroporous, non-ionic polymeric adsorbent boasts a large surface area, making it highly effective for the adsorption and separation of a wide range of organic compounds.
Researchers can leverage protocols found in literature, preprints, and patents to optimize their research processes and enhance reproducibility and accuracy when using Diaion HP 20.
By utilizing AI-driven comparisons, scientists can identify the best protocols and products for their specific needs, improving the efficacy and efficiency of their studies.
Diaion HP-20, a similar adsorbent resin, has also been extensively used in various applications, including the purification and separation of natural products, such as those found in Sephadex LH-20.
Additionally, RP-18 F254S and Silica gel 60 are other adsorbents that can be used in conjunction with or as alternatives to Diaion HP 20, depending on the specific requirements of the research project.
This versitale adsorbent resin continues to be an invaluable tool in the pursuit of scientific discovery and innovation, enabling researchers to optimize their workflows and enhance the quality and reliability of their results.