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Diethylamine

Diethylamine is a colorless, volatile, flammable liquid with a fishy, ammonia-like odor.
It is used as a chemical intermediate in the production of pharmaceuticals, pesticides, and other industrial chemicals.
Diethylamine can also be found in some natural products and is a component of certain tobacco smoke constituents.
Exposure to diethylamine may cause irritation to the eyes, skin, and respiratory tract.
Proper safety precautions should be taken when handling this substance.

Most cited protocols related to «Diethylamine»

1
Measurement and Analysis of Amyloid-Beta in Tissues
Cited 13 times

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Publication 2010
2
Synthesis and Characterization of Ketamine Stereoisomers
Cited 10 times
R-ketamine hydrochloride and S-ketamine hydrochloride were prepared by recrystallization of RS-ketamine (Ketalar, ketamine hydrochloride, Daiichi Sankyo Pharmaceutical, Tokyo, Japan) and d-(−)-tartaric acid (or l- (+)-tartaric acid), as described previously.34 The purity of these stereoisomers was determined by a high-performance liquid chromatography (CHIRALPAK IA, column size: 250 × 4.6 mm, mobile phase: n-hexane/dichloromethane/diethylamine (75/25/0.1), Daicel, Tokyo, Japan). NBQX, 2,3-dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide (catalog number: 0373, Tocris Bioscience, Bristol, UK, 10 mg kg−1) was dissolved in saline. ANA-12, N2-(2-{[(2-oxoazepan-3-yl) amino]carbonyl}phenyl)benzo[b]thiophene-2-carboxamide (catalog number: BTB06525SC, Maybridge, Trevillett Tintagel, Cornwall, UK, 0.5 mg kg−1) was prepared in vehicle of 1% dimethylsulfoxide in phosphate-buffered saline. The dose of ketamine, NBQX and ANA-12 was selected as reported previously.34 , 35 , 36 (link), 37 (link), 38 (link), 39 (link), 40 (link) Other reagents were purchased commercially.
Yang C., Shirayama Y., Zhang J.C., Ren Q., Yao W., Ma M., Dong C, & Hashimoto K. (2015). R-ketamine: a rapid-onset and sustained antidepressant without psychotomimetic side effects. Translational Psychiatry, 5(9), e632-.
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Publication 2015
2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline diethylamine High-Performance Liquid Chromatographies Ketalar Ketamine Ketamine Hydrochloride Methylene Chloride n-hexane Pharmaceutical Preparations Phosphates Quinoxalines Saline Solution Stereoisomers Sulfonamides Sulfoxide, Dimethyl tartaric acid Thiophene
3
Comparative Analysis of HIV Incidence Assays
Cited 8 times
Each of the five assays measures an aspect of an individual's immune response, with measurements below some threshold interpreted as indicative of ‘recent’ infection.
BED [8 (link),23 ] and LAg [7 (link),24 ] (Sedia Biosciences Corporation, Portland, Oregon, USA) were developed specifically as incidence assays by the Centers for Disease Control and Prevention (CDC). The immunoglobulin G (IgG) capture BED enzyme immunoassay (EIA) measures the proportion of IgG that is specific to HIV, with a normalized optical density (ODn) below 0.8 indicating ‘recent’ infection. The single-well LAg EIA is responsive to the avidity of HIV-1-specific IgG, as it presents marginally low concentrations of a multisubtype recombinant HIV-1 antigen, typically affording just a single binding site to the multivalent IgG or IgM antibodies. Whereas a ‘recent’/‘nonrecent’ threshold of 1.0 ODn was initially proposed, this was recently revised to 1.5 [24 ,25 ], following a review of the assay in which CEPHIA participated.
Both Less-sensitive Vitros (LS-Vitros) and Vitros Avidity [9 (link)] are based on the VITROS ECi/ECiQ Immunodiagnostic System – a chemiluminescence assay that gives a quantitative measure of HIV antibodies (Ortho-Clinical Diagnostics, Inc., Rochester, New York, USA). For LS-Vitros, a reported signal-to-cut-off (S/C) below 20, for a diluted specimen, is interpreted as a ‘recent’ result. For Vitros Avidity, the ratio of the S/C in an aliquot treated with a chaotropic agent (guanidine) to the S/C value in an aliquot not thus treated yields an avidity index (AI). A ‘recent’/‘non-recent’ threshold of 60% on the AI is used to classifiy the infection.
The BioRad Avidity test [10 ] is based on a modification of the Genetic Systems HIV-1/HIV-2 plus O EIA (Bio-Rad Laboratories, Inc., Hercules, California, USA), and involves the testing of each specimen in the presence and absence of a chaotropic agent (diethylamine). The ratio of the reactivity of the treated to untreated aliquot produces an AI, with values below 40% indicating ‘recent’ infection.
All assays were applied according to developers’ standard operating procedures and package inserts [7 (link)–9 (link),23 ,24 ], and protocols are available on the CEPHIA project website [6 ]. Testing was performed independently in CEPHIA laboratories, by technicians trained by the test developers and blinded to specimen background information. Three large-volume ‘control’ specimens (obtained from blood donations, and chosen to represent a range of serological responses) were supplied to laboratory technicians with each panel, for regular confirmation of reproducibility and stability of assays.
Kassanjee R., Pilcher C.D., Keating S.M., Facente S.N., McKinney E., Price M.A., Martin J.N., Little S., Hecht F.M., Kallas E.G., Welte A., Busch M.P, & Murphy G. (2014). Independent assessment of candidate HIV incidence assays on specimens in the CEPHIA repository. AIDS (London, England), 28(16), 2439-2449.
Publication 2014
Antigens Binding Sites, Antibody Biological Assay Blood Donation Chemiluminescent Assays Diagnosis diethylamine Enzyme Immunoassay Gene Editing Guanidine HIV-1 HIV-2 HIV Antibodies Immunodiagnosis Immunoglobulin G Immunoglobulin M Infection Laboratory Technicians Response, Immune System 1 plus Vision
4
Quantifying Murine Aβ Levels by ELISA
Cited 6 times
Murine Aβ levels were determined by sandwich ELISA using aliquots of the 10% sucrose homogenate. Soluble Aβ from an aliquot of the sucrose homogenates of wild-type and Tg2576 mice was extracted by diethylamine (DEA) [48] (link); separately, formic-acid extraction of another aliquot of the sucrose homogenate was used to detect β-amyloid plaque-associated Aβ in the Tg2576 mice. In combination with Aβ40 and Aβ42 C-terminal specific monoclonal antibodies (capture antibodies JRF/cAβ40/10 and JRF/cAβ42/26) [49] (link), horseradish peroxidase-conjugated m3.2 was used to detect endogenous murine Aβ in Aβ40- and Aβ42-specific sandwich ELISAs [50] (link). Similar ELISAs using JRF/AβN25 [51] (link) were used to detect human Aβ in the Tg2576 mouse brains. ELISA results are reported as the mean ± SEM in fmol Aβ per g wet brain, based on standard curves using synthetic murine and human Aβ1−40 and Aβ1−42 peptide standards (American Peptide).
Morales-Corraliza J., Mazzella M.J., Berger J.D., Diaz N.S., Choi J.H., Levy E., Matsuoka Y., Planel E, & Mathews P.M. (2009). In Vivo Turnover of Tau and APP Metabolites in the Brains of Wild-Type and Tg2576 Mice: Greater Stability of sAPP in the β-Amyloid Depositing Mice. PLoS ONE, 4(9), e7134.
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Publication 2009
Antibodies Brain Brain Edema diethylamine Enzyme-Linked Immunosorbent Assay formic acid Homo sapiens Horseradish Peroxidase Mice, Laboratory Monoclonal Antibodies Mus Peptides Plaque, Amyloid Sucrose
5
Amyloid-beta Extraction and Quantification
Cited 6 times
Before extraction of Aβ from brain tissue, 10% (w/v) homogenates were prepared in tissue homogenization buffer (20 mm Tris base, pH 7.4, 250 mm sucrose, 1 mm EDTA, 1 mm EGTA) with 100 mm phenylmethylsulphonyl fluoride and protease inhibitors [protease inhibitors cocktail (Complete, Roche Diagnostic) plus pepstatin A] added immediately before homogenization, as we have previously published (Asuni et al., 2006 (link); Sadowski et al., 2006 (link); Scholtzova et al., 2008 (link)). For extraction of soluble Aβ, brain homogenates were thoroughly mixed with an equal volume of 0.4% diethylamine (DEA)/100 mm NaCl, then spun at 135,000 × g for 1 h at 4°C, and subsequently neutralized with 1/10 volume of 0.5 m Tris, pH 6.8. The samples were then aliquoted, flash-frozen on dry ice, and stored at -80°C until loaded onto ELISA plates. Similarly for extraction of the total Aβ, homogenates (200 μl) were added to 440 μl of cold formic acid (FA) and sonicated for 1 min on ice. Subsequently, 400 μl of this solution was spun at 100,000 × g for 1 h at 4°C. Then, 210 μl of the resulting supernatant was diluted into 4 ml of FA neutralization solution (1 m Tris base, 0.5 M Na2HPO4, 0.05% NaN3), aliquoted, flash-frozen on dry ice, and stored at -80°C until used for Aβ measurements.
The total and soluble Aβ levels were measured using a combination of mouse monoclonal antibody 6E10 (specific to an epitope present on amino acid residues 1-16 of Aβ) and two different rabbit polyclonal antibodies specific for Aβ40 (R162) and Aβ42 (R165), in a double-antibody sandwich ELISA as described previously (Sadowski et al., 2006 (link)). The optical density (OD) was measured at 450 nm. The relationship between OD and Aβ peptide concentration was determined by a four-parameter logistic log function. Nonlinear curve fitting was performed with the KinetiCalc program (Biotek Instruments) to convert OD of plasma to estimated concentrations. The assay was performed by an investigator blinded to group assignment. The levels of Aβ species are presented as μg of Aβ per g of wet brain, taking into account dilution factors introduced by multiple steps throughout the assay (brain homogenization and extraction procedures).
Scholtzova H., Kascsak R.J., Bates K.A., Boutajangout A., Kerr D.J., Meeker H.C., Mehta P.D., Spinner D.S, & Wisniewski T. (2009). Induction of Toll-Like Receptor 9 Signaling as a Method for Ameliorating Alzheimer's Disease-Related Pathology. The Journal of Neuroscience, 29(6), 1846-1854.
Publication 2009
Amino Acids Antibodies Biological Assay Brain Brain Edema Buffers Cold Temperature Diagnosis diethylamine Dry Ice Edetic Acid Egtazic Acid Enzyme-Linked Immunosorbent Assay Epitopes formic acid Freezing Immunoglobulins Mice, House Monoclonal Antibodies pepstatin Peptides Phenylmethylsulfonyl Fluoride Plasma Protease Inhibitors Rabbits Sodium Azide Sodium Chloride Sucrose Technique, Dilution Tissues Tromethamine Vision

Most recents protocols related to «Diethylamine»

1
Quantifying Aβ Peptides in Brain
Diethylamine (0.2%) and formic acid (70%) were used for extraction of soluble and insoluble Aβ peptides, respectively, from brain homogenates as described [11 (link)], which were then quantified by ELISA kits for the Aβ40 and Aβ42 species (ThermoFisher Scientific, #KHB3482 and #KHB3441, respectively, Waltham, MA, USA) according to the manufacturer’s protocol.
Mast N., Li Y, & Pikuleva I.A. (2024). 7,8-Dihydroxy Efavirenz Is Not as Effective in CYP46A1 Activation In Vivo as Efavirenz or Its 8,14-Dihydroxy Metabolite. International Journal of Molecular Sciences, 25(4), 2242.
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Publication 2024
2
Organic Compound Extraction and Analysis
Not available on PMC !
Concentrated sulfuric acid, Cyclohexane, 37% Formaldehyde solution, Concentrated ammonia, Diethylamine, Sodium carbonate, Sodium nitroprusside purchased from Fisher, UK, Acetaldehyde, Diphenylamine, anhydroussodium sulphate (Alpha chemika, India), Chloroform (Loba Chemie, India), Ethyl acetate (Sigma Aldrich, US), Methanol (AR) purchased from Sigma Aldrich, Chloroform (AR) were purchased from VWR, UK used for analysis process.
Jayasekera V.S. (2024). Identification of Precursors of MDMA in Unknown Substances in Sri Lanka. International Journal of Forensic Sciences, 9(1), 1-7.
Publication 2024
3
Synthesis of Functionalized Polymers
The PAN industrial was provided by Taekwang Industry (Korea). Dimethylformamide (DMF; ~ 99.98% purity, Sigma-Aldrich), Glycidyl methacrylate ( C7H10O3 ,  ≤ 97%, Sigma-Aldrich), methanol ( CH3OH , Merck), ethylenediamine (EDA > 99%, Merck), diethylamine (DEA > 99%, Merck), ethanolamine (EA > 99%, Merk), triethylamine (TEA > 99%, Merck), ferrous sulfate heptahydrate ( FeSO4.7H2O ,Sigma-Aldrich), sodium molybdate dihydrate ( Na2MoO4.2H2O ; ~ 99% purity, Sigma Aldrich), Deionized water (DI).
Fayazi M.R., Outokesh M., Asadollahzadeh M., Torab-Mostaedi M, & Torkaman R. (2024). Targeted elimination of molybdenum ions from a leaching solution with the ability of radiated grafting GMA-PAN nanofibers. Scientific Reports, 14, 252.
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Publication 2024
4
Quantifying Cerebral Amyloid-Beta
Cerebral Aβ was extracted from frozen hemibrains as described [27 (link)]. Briefly, soluble Aβ was first extracted with 0.2% diethylamine (DEA) followed by centrifugation at 100,000 x g, then insoluble Aβ in the pellet was extracted using guanidium isothiocyanate. Following neutralization and appropriate dilution as described [27 (link)], Aβ42 and Aβ40 were quantified using end-specific sandwich ELISAs (Wako Chemicals, Richmond, VA) [28 (link)].
Terron H.M., Parikh S.J., Abdul-Hay S.O., Sahara T., Kang D., Dickson D.W., Saftig P., LaFerla F.M., Lane S, & Leissring M.A. (2024). Prominent tauopathy and intracellular β-amyloid accumulation triggered by genetic deletion of cathepsin D: implications for Alzheimer disease pathogenesis. Alzheimer's Research & Therapy, 16, 70.
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Publication 2024
5
Quantifying c-di-GMP and DEA NONOate
c-di-GMP and diethylamine
(DEA) NONOate were
purchased from Cayman Chemical (Ann Arbor, MI). GMP and pGpG were
purchased from Sigma-Aldrich (St. Louis, MO) and BIOLOG Life Science
Institute (Bremen, Germany), respectively. All other chemicals were
acquired from Kanto Chemical (Tokyo, Japan), Nacalai Tesque (Kyoto,
Japan), or FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan),
were of the highest guaranteed grade available, and were used without
further purification.
Kitanishi K., Aoyama N, & Shimonaka M. (2024). Gas-Selective Catalytic Regulation by a Newly Identified Globin-Coupled Sensor Phosphodiesterase Containing an HD-GYP Domain from the Human Pathogen Vibrio fluvialis. Biochemistry, 63(4), 523-532.
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Publication 2024

Top products related to «Diethylamine»

1
Diethylamine by Merck Group
Sourced in United States, Germany, India, Italy
Diethylamine is a colorless, flammable liquid with a strong, amine-like odor. It is a widely used organic compound that serves as a versatile laboratory reagent. The core function of diethylamine is to act as a base and a nucleophile in various chemical reactions and synthesis procedures.
2
Methanol by Merck Group
Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan
Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
3
Acetonitrile by Merck Group
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Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.
4
Ethanol by Merck Group
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Ethanol is a clear, colorless liquid chemical compound commonly used in laboratory settings. It is a key component in various scientific applications, serving as a solvent, disinfectant, and fuel source. Ethanol has a molecular formula of C2H6O and a range of industrial and research uses.
5
Triethylamine by Merck Group
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Triethylamine is a clear, colorless liquid used as a laboratory reagent. It is a tertiary amine with the chemical formula (CH3CH2)3N. Triethylamine serves as a base and is commonly employed in organic synthesis reactions.
6
Formic acid by Merck Group
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Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.
7
Acetic acid by Merck Group
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Acetic acid is a colorless, vinegar-like liquid chemical compound. It is a commonly used laboratory reagent with the molecular formula CH3COOH. Acetic acid serves as a solvent, a pH adjuster, and a reactant in various chemical processes.
8
1260 hplc by Agilent Technologies
Sourced in United States, Germany
The Agilent 1260 HPLC is a high-performance liquid chromatography system designed for analytical separations and quantitative analysis. It features a modular design, allowing for customization to meet specific application requirements. The system is capable of delivering precise and reproducible mobile phase flow, ensuring reliable performance and data integrity.
9
Sodium hydroxide (naoh) by Merck Group
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Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.
10
Diethylamine nonoate by Cayman Chemical
Sourced in United States
Diethylamine NONOate is a chemical compound that serves as a source of nitric oxide (NO) upon decomposition. It is commonly used in biochemical research and laboratory applications that require the controlled generation of nitric oxide.

More about "Diethylamine"

Diethylamine is a colorless, volatile, and flammable liquid with a fishy, ammonia-like odor.
It is a common chemical intermediate used in the production of pharmaceuticals, pesticides, and other industrial chemicals.
Diethylamine can also be found in natural products and is a component of certain tobacco smoke constituents.
Exposure to diethylamine may cause irritation to the eyes, skin, and respiratory tract, so proper safety precautions should be taken when handling this substance.
Diethylamine is closely related to other commonly used chemicals like methanol, acetonitrile, ethanol, triethylamine, formic acid, acetic acid, and sodium hydroxide.
When conducting research on diethylamine, it's important to carefully select and optimize the experimental protocols.
PubCompare.ai is an AI-driven platform that can help you easily locate and compare protocols from literature, preprints, and patents, ensuring you identify the most accurate and reproducible methods for your diethylamine studies.
Whether you're working with diethylamine, diethylamine NONOate, or related compounds, PubCompare.ai can enhance your research by providing access to a wealth of information and enabling you to make informed decisions about your experimental approach.
Utilze this powerful tool to streamline your diethylmaine research and achieve the best possible results.