Bone marrow cells were collected from the femurs and tibiae of wild-type BALB/c mice (Taconic) by flushing the opened bones with Iscove's Modified Dulbecco's Medium (IMDM; Invitrogen). Red blood cells were lysed in dH2O followed by the addition of 10X PBS. After centrifugation, the cells were washed once in PBS containing 0.1% BSA. The bone marrow cells were cultured at 106/mL in media containing RPMI 1640 (Invitrogen) with 20% FBS (Cambrex), 100 IU/mL penicillin and 10 μg/mL streptomycin (Cellgro), 2 mM glutamine (Invitrogen), 25 mM HEPES and 1x non-essential amino acids and 1 mM sodium pyruvate (Gibco) and 50 μM β-mercaptoethanol (Sigma) and supplemented with 100 ng/mL stem-cell factor (SCF; PeproTech) and 100 ng/mL FLT3-Ligand (FLT3-L; PeproTech) from day 0 to day 4. On day 4, the media containing SCF and FLT3-L was replaced with media containing 10 ng/mL recombinant mouse interleukin-5 (rmIL-5; R&D Systems) only. On day 8, the cells were moved to new flasks and maintained in fresh media supplemented with rmIL-5. Every other day, from this point forward, one-half of the media was replaced with fresh media containing rmIL-5, and the concentration of the cells was adjusted each time to106 /mL. Cells were enumerated at day 0 and on days indicated thereafter in a hemocytometer, and 50,000 cells were subjected to cytospin (Thermo Shandon, Pittsburgh, PA). The cytospin preparations were fixed and stained using a modified Giemsa preparation (Diff Quik, Dade Behring AG, Dudingen, Switzerland).
>
Chemicals & Drugs
>
Organic Chemical
>
Diff Quik
Diff Quik
Diff Quik is a rapid, reliable staining method used for the differential staining of cells, particularly in cytological and hematological examinations.
It involves a series of dips in various staining solutions to differentially color cellular components, allowing for the identification and classification of cell types.
Diff Quik is widely used in clinical settings, research laboratories, and diagnostic procedures to provide quick and accurate cell analysis, supporting the diagnosis of various medical conditions.
Its ease of use, reproducibility, and ability to deliver clear cellular detail have made Diff Quik an indispensable tool for healthcare professionals and researchers working in the field of cell biology and pathology.
It involves a series of dips in various staining solutions to differentially color cellular components, allowing for the identification and classification of cell types.
Diff Quik is widely used in clinical settings, research laboratories, and diagnostic procedures to provide quick and accurate cell analysis, supporting the diagnosis of various medical conditions.
Its ease of use, reproducibility, and ability to deliver clear cellular detail have made Diff Quik an indispensable tool for healthcare professionals and researchers working in the field of cell biology and pathology.
Most cited protocols related to «Diff Quik»
2-Mercaptoethanol
3,3'-diallyldiethylstilbestrol
Amino Acids, Essential
Bone Marrow Cells
Bones
Cells
Centrifugation
Diff Quik
Erythrocytes
Femur
flt3 ligand
FLT3 protein, human
Glutamine
HEPES
Interleukin-5
Mice, Inbred BALB C
Mus
Penicillins
Pyruvate
Sodium
Stain, Giemsa
Stem Cell Factor
Streptomycin
Tibia
Liver tissue specimen was fixed in formalin and embedded in paraffin. Histological sections of 4 µm thickness were stained with hematoxylin and eosin using a standard protocol. The histopathological changes before and after L. major infection were observed using a Leica DM2500 (North Ryde, Australia). To assess the degree of inflammation, three representative inflammatory foci were imaged at 100× magnification. The number of inflammatory foci in each image was quantified and the average for each of the three representative areas per animal was then calculated.
For immunohistochemical staining, tissue sections were de-paraffinized in xylene and rehydrated. The antigens were retrieved in 10 mmol/l sodium citrate buffer (pH 9.0) for 10 min at 100°C and then cooled to room temperature before being stained. Endogenous peroxidase activity was quenched by treatment with 3% H2O2 in methanol for 10 min. The sections were blocked in protein block (X0909, Dako, VIC, Australia) for 30 min at room temperature and then incubated with a primary mAb to CD68 (ab31630, Abcam, VIC, Australia) for 1 h at 37°C. Immunoreactivity was visualized with diaminobenzidine (Dako) using the Envision system (Dako) according to the manufacturer’s protocol. The nuclei were lightly counterstained with hematoxylin solution. A negative control was prepared using the same staining procedure but was not incubated with the abovementioned primary antibodies. Images were obtained using an Olympus BX53 microscope (Olympus), and semiquantitative analysis was conducted using Image-Pro Plus software (ImageJ, USA).
Flow cytometrically sorted cells were centrifuged on slides in a Cytospin™ 4 Cytocentrifuge (Thermo Fisher Scientific) at 500 g for 10 min and underwent microscopical analysis after air-drying and staining with Diff-Quik reagent.
For immunohistochemical staining, tissue sections were de-paraffinized in xylene and rehydrated. The antigens were retrieved in 10 mmol/l sodium citrate buffer (pH 9.0) for 10 min at 100°C and then cooled to room temperature before being stained. Endogenous peroxidase activity was quenched by treatment with 3% H2O2 in methanol for 10 min. The sections were blocked in protein block (X0909, Dako, VIC, Australia) for 30 min at room temperature and then incubated with a primary mAb to CD68 (ab31630, Abcam, VIC, Australia) for 1 h at 37°C. Immunoreactivity was visualized with diaminobenzidine (Dako) using the Envision system (Dako) according to the manufacturer’s protocol. The nuclei were lightly counterstained with hematoxylin solution. A negative control was prepared using the same staining procedure but was not incubated with the abovementioned primary antibodies. Images were obtained using an Olympus BX53 microscope (Olympus), and semiquantitative analysis was conducted using Image-Pro Plus software (ImageJ, USA).
Flow cytometrically sorted cells were centrifuged on slides in a Cytospin™ 4 Cytocentrifuge (Thermo Fisher Scientific) at 500 g for 10 min and underwent microscopical analysis after air-drying and staining with Diff-Quik reagent.
Animals
Antibodies
Antigens
Buffers
Cell Nucleus
Cells
Diff Quik
Eosin
Formalin
Hematoxylin
Infection
Inflammation
Liver
Methanol
Microscopy
Paraffin Embedding
Peroxidase
Peroxide, Hydrogen
Proteins
Sodium Citrate
Tissues
Xylene
Ixodes scapularis-derived ISE6 cells, provided by T. Kurtti (University of Minnesota), were maintained in antibiotic-free L15B growth medium supplemented with 10% heat-inactivated fetal bovine serum (HyClone), 10% tryptose phosphate broth (Becton, Dickinson and Company) [40] (link), at pH 6.8–7.0 in a humidified 5% CO2 incubator at 32°C. For rickettsial isolation, two pools of 25 L. bostrychophila were sequentially washed over filter paper as follows: three times in 70% ethanol, one time in 10% sodium hypochlorite, and rinsed in sterile distilled water. Booklice pools were either transferred by pipette tip to a sterile glass pestle tissue grinder and ground in 25 µl of L15B tick cell culture media or directly transferred to a 25-cm2 tissue culture flask containing ISE6 cells (passage 134). ISE6 cells with booklice preparations were immediately placed at 32°C. For regular maintenance of Rickettsia-infected cells, they were passed at a ratio of 1∶5 every 21 to 28 days. Upon each passage of ISE6 cells exposed to booklice homogenates, a portion of the cells were prepared for staining using a Cytospin centrifuge (Wescor) and rickettsial infection was assessed by traditional PCR [15] (link) and/or Diff-Quik (Dade Behring) staining, according to the manufacturer's protocol.
3,3'-diallyldiethylstilbestrol
Antibiotics
Cell Culture Techniques
Cells
Culture Media
Diff Quik
Ethanol
Fetal Bovine Serum
isolation
Ixodes scapularis
Phosphates
Rickettsia
Rickettsia Infections
Sodium Hypochlorite
Sterility, Reproductive
Strains
Ticks
Tissues
tryptose
Persons at least 12 years of age who reported a physician’s diagnosis of asthma were recruited as study participants in Sarasota, Florida, an area with a history of annual Florida red tides. All participants who gave informed consent to participate walked on the beach once during a Florida red tide and also walked once when no Florida red tide was present. Participants were instructed to maintain their daily regime for asthma control. They were asked to spend a minimum of 1 hr at the beach in areas where environmental monitoring was ongoing and were told they could leave the beach at any time if they felt uncomfortable or symptomatic and could freely use any personal medications. Study activities included the pre- and postexposure questionnaires, swab sampling, and spirometry, as well as carrying a personal air monitor while at the beach.
Questionnaires were administered upon enrollment and then before and after the participants visited the beach. The questionnaires collected information about demographics, baseline pulmonary health history, prior experience with Florida red tide, medications, potential confounders, and symptoms. In addition to asking about expected common respiratory symptoms, the questionnaire asked about diarrhea to detect overreporting bias, because diarrhea was not expected to be associated with exposure to aerosolized Florida red tide (vs. ingestion exposure resulting in NSP). For the purposes of analysis, age groups were evaluated as tertiles (< 18 years, 18–60 years, > 60 years). Geographic proximity of residence to the beach also was explored; proximity was defined as residence on a barrier island or along Sarasota Bay (i.e., within approximately 1 mile of the seashore). The investigators considered two surrogate measures of asthma severity: above and below the unexposed period study population mean forced expiratory volume in 1 sec (FEV1) and the use of asthma medications (predominantly β 2 agonists) within 12 hr before going to the beach. However, the mean FEV1 before the unexposed beach visit was not considered to be a good measure of disease severity and was not used: no untreated baseline was obtained before the unexposed beach visit, and in the study population, persons who used asthma medication within 12 hr before going to the beach, were more likely to have an FEV1 value greater than the mean FEV1 from the unexposed period (data not shown). Therefore, only the use of asthma medications within 12 hr before going to the beach was used as a surrogate for increased asthma severity.
Spirometry tests were performed using a portable OMI2000 10-L dry-rolling-seal volume spirometer (Occupational Marketing, Inc., Houston, TX) by personnel trained according to the standards of the National Institute for Occupational Safety and Health (NIOSH 1997 ). The spirometric values of interest were FEV1, forced vital capacity (FVC), forced expiratory flow between 25 and 75% (FEF25–75, peak expiratory flow (PEF), and FEV1/FVC percentage. For the purposes of this study, each participant served as his or her own spirometry control (i.e., pre-exposure/postexposure); all study participants had at least three reproducible spirograms before and after visiting the beach. The data were considered adequate if they conformed to standard guidelines for the collection and interpretation of spirometry measurements (American Thoracic Society 1991 (link), 1995 (link); Hankinson et al. 1999 (link)).
As an effect biomarker of inflammation, nose and throat swabs were collected from the study participants before and after they went to the beach. Samples were obtained by gently wiping the nose or throat with a cotton-tipped swab, smearing the material onto duplicate microscope slides, and fixing with a cytologic adhesive spray (Spray-Cyte; Becton Dickinson, Sparks, MD). One slide from each pair was stained using Diff Quik (Dade Behring Inc., Newark, DE) for cytologic evaluation of epithelial and inflammatory cells. The inflammatory response was characterized according to cellularity and the percentage of neutrophils and chronic inflammatory cells (e.g., macrophages, lymphocytes, and plasma cells). In addition, protein transudation and amount of fibrin present were evaluated because increasingly permeable cell membranes, a key event in the inflammatory process, lead to protein transudation as proteins leak out of the cells. As described above, brevetoxin levels also were analyzed by a newly developed brevetoxin ELISA (Naar et al. 2002 (link)) on the nasal and throat swabs as an exposure biomarker.
Questionnaires were administered upon enrollment and then before and after the participants visited the beach. The questionnaires collected information about demographics, baseline pulmonary health history, prior experience with Florida red tide, medications, potential confounders, and symptoms. In addition to asking about expected common respiratory symptoms, the questionnaire asked about diarrhea to detect overreporting bias, because diarrhea was not expected to be associated with exposure to aerosolized Florida red tide (vs. ingestion exposure resulting in NSP). For the purposes of analysis, age groups were evaluated as tertiles (< 18 years, 18–60 years, > 60 years). Geographic proximity of residence to the beach also was explored; proximity was defined as residence on a barrier island or along Sarasota Bay (i.e., within approximately 1 mile of the seashore). The investigators considered two surrogate measures of asthma severity: above and below the unexposed period study population mean forced expiratory volume in 1 sec (FEV1) and the use of asthma medications (predominantly β 2 agonists) within 12 hr before going to the beach. However, the mean FEV1 before the unexposed beach visit was not considered to be a good measure of disease severity and was not used: no untreated baseline was obtained before the unexposed beach visit, and in the study population, persons who used asthma medication within 12 hr before going to the beach, were more likely to have an FEV1 value greater than the mean FEV1 from the unexposed period (data not shown). Therefore, only the use of asthma medications within 12 hr before going to the beach was used as a surrogate for increased asthma severity.
Spirometry tests were performed using a portable OMI2000 10-L dry-rolling-seal volume spirometer (Occupational Marketing, Inc., Houston, TX) by personnel trained according to the standards of the National Institute for Occupational Safety and Health (NIOSH 1997 ). The spirometric values of interest were FEV1, forced vital capacity (FVC), forced expiratory flow between 25 and 75% (FEF25–75, peak expiratory flow (PEF), and FEV1/FVC percentage. For the purposes of this study, each participant served as his or her own spirometry control (i.e., pre-exposure/postexposure); all study participants had at least three reproducible spirograms before and after visiting the beach. The data were considered adequate if they conformed to standard guidelines for the collection and interpretation of spirometry measurements (American Thoracic Society 1991 (link), 1995 (link); Hankinson et al. 1999 (link)).
As an effect biomarker of inflammation, nose and throat swabs were collected from the study participants before and after they went to the beach. Samples were obtained by gently wiping the nose or throat with a cotton-tipped swab, smearing the material onto duplicate microscope slides, and fixing with a cytologic adhesive spray (Spray-Cyte; Becton Dickinson, Sparks, MD). One slide from each pair was stained using Diff Quik (Dade Behring Inc., Newark, DE) for cytologic evaluation of epithelial and inflammatory cells. The inflammatory response was characterized according to cellularity and the percentage of neutrophils and chronic inflammatory cells (e.g., macrophages, lymphocytes, and plasma cells). In addition, protein transudation and amount of fibrin present were evaluated because increasingly permeable cell membranes, a key event in the inflammatory process, lead to protein transudation as proteins leak out of the cells. As described above, brevetoxin levels also were analyzed by a newly developed brevetoxin ELISA (Naar et al. 2002 (link)) on the nasal and throat swabs as an exposure biomarker.
3,3'-diallyldiethylstilbestrol
Age Groups
agonists
Asthma
Biological Markers
brevetoxin
Brevetoxin A
Cells
Conditioning, Psychology
Cytological Techniques
Diagnosis
Diarrhea
Diff Quik
Enzyme-Linked Immunosorbent Assay
Exhaling
Feelings
Fibrin
Forced Vital Capacity
Gossypium
Inflammation
Lung
Lymphocyte
Macrophage
Microscopy
Neutrophil
Nose
Permeability
Pharmaceutical Preparations
Pharynx
Phocidae
Physicians
Plasma Cells
Plasma Membrane
Proteins
Red Tide
Signs and Symptoms, Respiratory
Spirometry
Volumes, Forced Expiratory
DEPs were sterilized by autoclaving and suspended in serumfree media after coating with BSA to minimize particle aggregation and hydrophobicity.22 (link) After sonication, the endotoxin concentration of the DEP suspension was <0.064 ng/mL (0.32 EU/mL) using the Limulus Amebocyte Lysate assay (QCL-1000; BioWhittaker Inc., Walkersville, MD, USA).23 (link) Female Balb/c mice, 5 to 6 weeks of age and free of mice-specific pathogens, were obtained from Orient Co, Ltd (Charles River Laboratories, Seoul, Korea). The mice were housed throughout the experiments in a laminar flow cabinet and maintained on standard laboratory chow ad libitum. All experimental animals used in this study were treated according to guidelines approved by the Institutional Animal Care and Use Committee of the Soonchunhyang University Medical School. DEPs were resuspended in saline solution for 30 minutes before administration. The mice (n=8 in each group) were exposed to 100 µg/m3 and 3 mg/m3 DEPs for 1 hour a day for 5 days a week from 4 to 12 weeks (Fig. 1 ) in a closed-system chamber attached to an ultrasonic nebulizer (NE-UO7; Omron Corporation, Tokyo, Japan) with an output of 1 mL/min and 1- to 5-µm particle size.
The control mice were administered and exposed to saline solution alone. Mice were sacrificed with an overdose of pentobarbital sodium (65 mg/kg body weight, administered intraperitoneally). The chest cavity was exposed, and the catheter was carefully inserted into the trachea and secured with ligatures. Bronchoalveolar lavage (BAL) was performed by 4 instillations of 1 mL of normal saline and gentle retrieval. Cell numbers were measured using a hemocytometer, and differential cell counts were performed on slides prepared by cyto-centrifugation and Diff-Quik staining (Scientific Products, Gibbstowne, NJ, USA). Supernatants were separated by centrifugation (500 g, 5 minutes) and maintained at -70℃ until use. After ligation of the right main bronchus, the left lung was fixed with 4% paraformaldehyde in phosphate-buffered saline and paraffin-embedded. The right lung was excised and immersed in TRI reagent (guanidinium thiocyanate-phenol mixture; Molecular Research Center Inc., Cincinnati, OH, USA), and immediately frozen in liquid nitrogen.
The control mice were administered and exposed to saline solution alone. Mice were sacrificed with an overdose of pentobarbital sodium (65 mg/kg body weight, administered intraperitoneally). The chest cavity was exposed, and the catheter was carefully inserted into the trachea and secured with ligatures. Bronchoalveolar lavage (BAL) was performed by 4 instillations of 1 mL of normal saline and gentle retrieval. Cell numbers were measured using a hemocytometer, and differential cell counts were performed on slides prepared by cyto-centrifugation and Diff-Quik staining (Scientific Products, Gibbstowne, NJ, USA). Supernatants were separated by centrifugation (500 g, 5 minutes) and maintained at -70℃ until use. After ligation of the right main bronchus, the left lung was fixed with 4% paraformaldehyde in phosphate-buffered saline and paraffin-embedded. The right lung was excised and immersed in TRI reagent (guanidinium thiocyanate-phenol mixture; Molecular Research Center Inc., Cincinnati, OH, USA), and immediately frozen in liquid nitrogen.
1-(2-(dodecyloxy)ethyl)pyrrolidine hydrochloride
Animals, Laboratory
Asian Persons
Biological Assay
Body Weight
Bronchoalveolar Lavage
Bronchus, Primary
Catheters
Centrifugation
Diff Quik
Drug Overdose
endotoxin binding proteins
Endotoxins
Females
Freezing
guanidine thiocyanate
Institutional Animal Care and Use Committees
Ligation
Ligature
Limulus
Lung
Mice, Inbred BALB C
Mus
Nebulizers
Nitrogen
Normal Saline
Paraffin
paraform
Pentobarbital Sodium
Phenols
Phosphates
PTPRJ protein, human
Rivers
Saline Solution
Specific Pathogen Free
Thoracic Cavity
Trachea
Ultrasonics
Most recents protocols related to «Diff Quik»
Blood was collected from participants during their PICU admission through an existing peripheral intravenous catheter with blood return or a central venous catheter into an ethylenediaminetetraacetic acid (EDTA) vacutainer tube. Tubes were centrifuged at 400ΧG to separate cells from platelet-rich plasma. Pelleted blood cells were resuspended in sterile phosphate buffered saline (PBS; ThermoFisher Scientific, Waltham, MA) with 2.5 mM EDTA up to the original whole blood volume. Neutrophils were negatively selected from PBS-EDTA washed whole blood using the EasySep Direct Human Neutrophil Isolation kit (StemCell Technologies, Cambridge, MA) according to the manufacturer’s protocol. Neutrophil purity was > 99% as confirmed by microscopic analysis of a Diff-Quik stained CytoSpin sample of purified neutrophils. Two million neutrophils were resuspended in 1 mL of RNALater and stored at − 80 °C until batch RNA purification.
Acids
BLOOD
Blood Cells
Blood Volume
Catheters
Cells
Diff Quik
Edetic Acid
Homo sapiens
isolation
Microscopy
Neutrophil
Phosphates
Platelet-Rich Plasma
Saline Solution
Stem Cells
Sterility, Reproductive
Venous Catheter, Central
During sacrifice under anesthesia, mice were subjected to blunt dissection of the trachea. A small-caliber tube was inserted into the airway; 0.9 ml of phosphate-buffered saline (PBS) was injected into the lungs. The fluid was collected, and the collection process was repeated to obtain 1.8 ml BAL fluid. The collected BAL fluid was centrifuged at 3000 rpm for 5 min at 4 °C to separate the cell pellet and supernatant. The cell pellet was resuspended in PBS and mixed with an equal volume of Trypan Blue Solution, and the cells were then counted using a hemocytometer. BAL cells were centrifuged onto slides using a Cytospin centrifuge (Thermo Fisher Scientific, Waltham, MA). Cell differentiation was assessed using a Diff-Quik stain kit (Medion Diagnostics, Fribourg, Switzerland). The supernatant was stored at − 80 °C and used for various protein analyses and assessment of LDH activity.
Anesthesia
Cells
Diagnosis
Differentiations, Cell
Diff Quik
Dissection
Lung
Mus
Phosphates
Proteins
Saline Solution
Trachea
Trypan Blue
Differential BAL white cell counts were completed using a variant of the Romanowsky–Giemsa stain, Diff-Quik. Superfrost™ Plus microscope slides were mounted to a Shandon cytospin system and were placed into a Cytospin 4 chamber (all Thermo Fisher) before 150 µL of BALF was added to the funnel. Samples were spun at 200 RPM for 5 min before the slide was removed from the chamber. After air drying for 5 min, the slides were fixed and stained by immersing them 6 times in methanol, 5 times in Eosin Y, 3 times in methylene blue, 5 times in a buffered solution, and 5 times in a rinse solution before being left to dry. After drying, one drop of DPX mountant (VWR International, Dublin, Ireland) was placed on each section and a cover slip (Thermo Fisher) was placed over and allowed to dry. Stained cells were visualised under light microscopy using a BX43 Olympus microscope (Olympus Life Sciences, Tokyo, Japan), and images were taken with a GXCAM-EYE-5 microscope attachment (GT Vision Ltd. Sudbury, Newmarket, UK). Differential morphological analysis was performed on the images to determine monocytes/macrophages. A total of 300 cells across at least 3 images were analysed per sample.
A 300
Cells
Diff Quik
Eosin
Leukocyte Counts, Differential
Light Microscopy
Macrophage
Methanol
Methylene Blue
Microscopy
Monocytes
Romanowsky-Giemsa stain
Vision
Immunobeads (Irvine Scientific, California, CA, USA), bovine blood (Japan Bio Serum Co. Ltd., Fukuyama, Japan), and heparin-treated human blood (Rockland Immunochemicals Inc., Pennsylvania, PA, USA) were purchased. Bovine blood cells and human blood cells were stained using the Wright–Giemsa stain kit (ScyTec Laboratories Inc., Utah, UT, USA) and Diff-Quik stain kit (Sysmex, Kobe, Japan), respectively. The running buffer was prepared using 0.5% (w/v) bovine serum albumin (BSA; Nacalai Tesque, Kyoto, Japan), and 2 mM EDTA (Dojindo, Kumamoto, Japan) in 10 mM PBS (Wako, Tokyo, Japan).
BLOOD
Blood Cells
Bos taurus
Buffers
Diff Quik
Edetic Acid
Heparin
Homo sapiens
Serum
Serum Albumin, Bovine
Stain, Giemsa
Rickettsia conorii strain Malish7 was grown in Vero cells, purified, and stored at −80 °C, as previously described [49 (link),50 (link)]. Briefly, monolayers of Vero cells were infected with a seed stock of R. conorii (MOI = 1) and grown at 35 °C, 5% CO2 in Dulbecco’s Modified Eagle medium (Coring, Manassas, VA, USA) containing 2% fetal bovine serum (FBS) (HyClone, Logan, UT, USA) until ~10–15% of the monolayer was lysed or detached (~4–5 days post-infection) from the surface. The Vero cells containing the bacteria were harvested, and R. conorii was purified by differential centrifugation. The purified rickettsial stock was suspended in K36 buffer (100 mM potassium chloride, 15 mM sodium chloride, 50 mM potassium phosphate buffer [pH 7.0]), aliquoted in <500 µL, and stored at −80 °C. The homogeneity of purified rickettsial stock was assessed using Diff-Quik staining (Siemens, Newark, DE, USA) and quantified via citrate synthase (gltA)-based qPCR and plaque assays, as described [24 (link),49 (link)].
E. coli strains DH5α and TOP10 F’ (ThermoFisher Scientific, Waltham, MA, USA) were grown in LB medium at 37 °C unless otherwise stated. All E. coli stocks were stored in 15% glycerol at −80 °C.
E. coli strains DH5α and TOP10 F’ (ThermoFisher Scientific, Waltham, MA, USA) were grown in LB medium at 37 °C unless otherwise stated. All E. coli stocks were stored in 15% glycerol at −80 °C.
Bacteria
Biological Assay
Buffers
Centrifugation
Citrate (si)-Synthase
Dental Plaque
Diff Quik
Eagle
Escherichia coli
Fetal Bovine Serum
Glycerin
Infection
Potassium Chloride
potassium phosphate
Rickettsia
Rickettsia conorii
Sodium Chloride
Strains
Vero Cells
Top products related to «Diff Quik»
Sourced in Japan
Diff-Quik is a staining system used for rapid differential staining of blood and other cell samples. It provides a quick and reliable way to identify and differentiate various cell types in a sample.
Sourced in Japan
The Diff-Quik kit is a quick and easy-to-use staining solution used for the differential staining of blood smears. It consists of three solutions that allow for the visualization of various blood cell types, including red blood cells, white blood cells, and platelets.
Sourced in Germany, United States, Italy, Switzerland
Diff-Quik is a three-step staining method used in hematology and cytology for the rapid differentiation and identification of blood cells and other cellular components. The stain provides a quick and reliable way to assess cellular morphology and differential cell counts.
Sourced in United States, United Kingdom, Germany, Japan, China, Spain, Italy, Canada
The BD BioCoat Matrigel Invasion Chambers are a laboratory equipment used to assess the invasive potential of cells. The chambers are pre-coated with Matrigel basement membrane matrix, which acts as a barrier to mimic the extracellular matrix. Researchers can use these chambers to study the ability of cells to invade through this barrier.
Sourced in United States, Germany
The Diff-Quik Stain Set is a set of stains used for the rapid differential staining of blood and other cytological specimens. It consists of three solutions: a fixative, a red stain, and a blue stain. The stain set is designed to provide a quick and reliable way to differentiate between different cell types in a sample.
Sourced in United States
Diff-Quik is a rapid, three-step staining method used for the differential staining of blood smears and other cytological specimens. It provides a quick and reliable way to obtain detailed information about the cellular composition of a sample.
Sourced in United States, United Kingdom, Germany, China, Canada, Japan, Italy, France, Belgium, Australia, Uruguay, Switzerland, Israel, India, Spain, Denmark, Morocco, Austria, Brazil, Ireland, Netherlands, Montenegro, Poland
Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. It is widely used as a substrate for the in vitro cultivation of cells, particularly those that require a more physiologically relevant microenvironment for growth and differentiation.
Sourced in United States
Diff-Quik is a staining set used for the rapid differential staining of blood and other cellular samples. It consists of three solutions that are applied sequentially to prepare slides for microscopic examination. The core function of Diff-Quik is to enable the clear visualization and identification of cellular components, which is crucial for various diagnostic and research applications.
Sourced in Japan
The Diff-Quik stain kit is a laboratory product used for the staining and differentiation of blood cells. It is a quick, three-step staining method that allows for the identification and enumeration of various cell types in blood smear samples.
Sourced in Japan
The Diff-Quik solution is a staining kit used for the rapid differential staining of blood smears and other cytological specimens. It is a quick and reliable method for the identification and enumeration of various blood cell types.