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Dimethylformamide

Dimethylformamide is a versatile organic solvent commonly used in chemical synthesis, pharmaceutical research, and material science.
It is a colourless, flammable liquid with a distinctive fishy odor.
Dimethylformamide has a high boiling point and is miscible with water, making it a useful solvent for a variety of organic and inorganic compounds.
Researchers can use PubCompare.ai's AI-driven platform to optimize their Dimethylformaide research by easily locating protocols from literature, preprints, and patents, and using AI-driven comparisons to identify the best protocols and products for their experiments.
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Most cited protocols related to «Dimethylformamide»

1
Transcriptome Dynamics via 4sU-Seq
Total RNA was extracted with the miRNeasy kit’s procedure (Qiagen), and sample quality was tested on a 2100 Bioanalyzer (Agilent). For the high-resolution (signature) analysis, we extracted RNA starting at 0h and until 3h after LPS stimulation in 15 minutes intervals. We generated replicated samples for the 0, 30, 45, 60 and 75 minutes samples. For the lower resolution analysis (4sU-Seq), we extracted RNA starting 0h and until 6h after LPS stimulation in 60 minutes intervals.
We used 20 μg total RNA for the biotinylation reaction. 4sU-labeled RNA was biotinylated using EZ-Link Biotin-HPDP (Pierce), dissolved in dimethylformamide (DMF) at a concentration of 1 mg/mL, and stored at -80°C. Biotinylation was done in labeling buffer (10 mM Tris pH 7.4, 1 mM EDTA) and 0.2 mg/mL Biotin-HPDP for 2h at room temperature. Unbound Biotin-HPDP was removed by chloroform/isoamylalcohol (24:1) extraction using MaXtract (high density) tubes (Qiagen). RNA was precipitated at 20,000g for 20 min with a 1:10 volume of 5M NaCl and an equal volume of isopropanol. The pellet was washed with an equal volume of 75% ethanol and precipitated again at 20,000g for 10 min. The pellet was re-suspended in 100 μL RNAse-free water. Biotinylated RNA was captured using Dynabeads MyOne™ Streptavidin T1 beads (Invitrogen). Biotinylated RNA was incubated with 100 μL Dynabeads with rotation for 15 min at room temperature. Beads were magnetically fixed and washed with 1x Dynabeads washing buffer. Flow-through was collected for unlabeled preexisting RNA recovery. RNA-4sU was eluted with 100 μL of freshly prepared 100 mM dithiothreitol (DTT). RNA was recovered from eluates and washing fractions with RNeasy MinElute Spin columns (Qiagen).
Rabani M., Levin J.Z., Fan L., Adiconis X., Raychowdhury R., Garber M., Gnirke A., Nusbaum C., Hacohen N., Friedman N., Amit I, & Regev A. (2011). Metabolic labeling of RNA uncovers principles of RNA production and degradation dynamics in mammalian cells. Nature biotechnology, 29(5), 436-442.
Publication 2011
Biotin Biotinylation Buffers Chloroform Dimethylformamide Dithiothreitol Edetic Acid Endoribonucleases Ethanol Isopropyl Alcohol N-(6-(biotinamido)hexyl)-3'-(2'-pyridyldithio)propionamide Sodium Chloride Streptavidin Tromethamine
2
TUNEL Assay for Detecting DNA Strand Breaks
DNA strand breaks were demonstrated by labeling free 3′-OH termini with FITC-labeled deoxyuridine, which was detected with alkaline phosphatase–coupled, anti-fluorescein antibody, and the formation of a dye precipitate with a phosphatase substrate (In Situ Cell Death Detection Kit, AP; Boehringer Mannheim, Mannheim, Germany). Yeast cells were fixed with 3.7% formaldehyde, digested with lyticase, and applied to a polylysine-coated slide as described for immunofluorescence (Adams and Pringle, 1984 (link)). The slides were rinsed with PBS, incubated in permeabilization solution (0.1% Triton X-100, 0.1% sodium citrate) for 2 min on ice, rinsed twice with PBS, incubated with 10 μl TUNEL reaction mixture (200 U/ml terminal deoxynucleotidyl transferase, 10 mM FITC-labeled dUTP, 25 mM Tris/HCl, 200 mM sodium cacodylate, 5 mM cobalt chloride; Boehringer Mannheim) for 60 min at 37°C, rinsed three times with PBS, incubated with 50 μl Converter AP solution (alkaline phosphatase– labeled, anti-FITC antibody; Boehringer Mannheim) for 30 min at 37°C, rinsed three times with PBS, and stained by incubation with 50 μl naphthol) AS-MX phosphate (Sigma Chemical Co., Munich, Germany), 0.8 mg/ml, fast red TR salt (Sigma Chemical Co.), 1 mg/ml, 2% dimethylformamide, 1 mM levamisole in 100 mM Tris/HCl, pH 8.2, for 30 min at room temperature. A coverslip was mounted with a drop of Kaiser's glycerol gelatin (Merck, Darmstadt, Germany).
Madeo F., Fröhlich E, & Fröhlich K.U. (1997). A Yeast Mutant Showing Diagnostic Markers of Early and Late Apoptosis. The Journal of Cell Biology, 139(3), 729-734.
Publication 1997
Alkaline Phosphatase Antibodies, Anti-Idiotypic Cacodylate Cell Death Cells cobaltous chloride Deoxyuridine deoxyuridine triphosphate Dimethylformamide DNA Breaks DNA Nucleotidylexotransferase fast red TR salt Fluorescein Fluorescein-5-isothiocyanate Fluorescent Antibody Technique Formaldehyde Gelatins Glycerin In Situ Nick-End Labeling Levamisole Hydrochloride lyticase Naphthols Phosphates Phosphoric Monoester Hydrolases Polylysine Sodium Sodium Citrate Triton X-100 Tromethamine Yeast, Dried
3
Quantifying Macrophage Metabolic Activity
THP-1 monocytes were seeded at 180 000 cells/well in 24 well plates and differentiated in macrophages as described. After incubation with IFN-γ ± LPS, cells were incubated 2 h with 500 μl of MTT reagent (2.5 mg/ml of PBS, Sigma #M2128) in the CO2 incubator. The media were then removed and 1 ml of lysis buffer (SDS 30 %/N,N-dimethyl-formamide 2:1 pH 4.7) was added per well. Plates were incubated at 37 °C and gently shaked at 70 rpm for 1 h. The absorbance was then measured at 570 nm.
Genin M., Clement F., Fattaccioli A., Raes M, & Michiels C. (2015). M1 and M2 macrophages derived from THP-1 cells differentially modulate the response of cancer cells to etoposide. BMC Cancer, 15, 577.
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Publication 2015
Buffers Cells Dimethylformamide Interferon Type II Macrophage Monocytes
4
Zebrafish Embryo Toxicity Assay
Zebrafish embryos of the AB wild-type strain (originally obtained from the Zebrafish International Resource Center, Eugene, Oregon, USA) were raised at 28°C. Zebrafish husbandry, embryo collection, and embryo and larva maintenance were performed as described [20] , [21] . Toxicity assays were standardly performed in 24-well microtiter plates (wrapped with Parafilm to limit solvent evaporation) using 10 embryos per well in 1 ml of 0.3× Danieau's medium (17 mM NaCl, 2 mM KCl, 0.12 mM MgSO4, 1.8 mM Ca(NO3)2 and 1.5 mM HEPES, pH 7.6). Each experiment was repeated 3 times for a total of 30 embryos or larvae analyzed per solvent per developmental staged tested. Data were only recorded for experiments in which the percentage of normal embryos or larvae in the control group was at least 90%. Embryos and larvae were exposed to solvents and carriers at 2–4 cells, 4 hpf, and at 1, 2, 3, 4, and 7 dpf and evaluated for signs of toxicity 24 hours later. In determining the maximum tolerated concentration (MTC) for each solvent and carrier, all post-exposure embryos and larvae were allowed to develop in larva medium to 9 dpf, so as to detect any deleterious effects appearing after this 24-hour window. Solvents and carriers were obtained from the following suppliers: acetone (Chemlab, Zedelgem, Belgium), acetonitrile (Acros Organics, Geel, Belgium), albumin (BSA, Sigma-Aldrich, Bornem, Belgium), butanone (Riedel-de Haën, Seelze, Germany), cyclodextrin (2- hydroxypropyl-beta-cyclodextrin, Sigma-Aldrich), dimethyl formamide (Acros), DMSO (Agros), ethanol (Fisher Scientific, Doornik, Belgium), glycerol (Acros), isopropanol (Chemlab), methanol (Chemlab), polyethylene glycol-400 (Fluka, Bornem, Belgium), propylene glycol (Certa, Eigenbrakel, Belgium), solketal (Merck, Overijse, Belgium). Statistical analyses were done using chi-square in Microsoft Excel.
Maes J., Verlooy L., Buenafe O.E., de Witte P.A., Esguerra C.V, & Crawford A.D. (2012). Evaluation of 14 Organic Solvents and Carriers for Screening Applications in Zebrafish Embryos and Larvae. PLoS ONE, 7(10), e43850.
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Publication 2012
2-Hydroxypropyl-beta-cyclodextrin Acetone acetonitrile Albumins Biological Assay Cells Certa Cyclodextrins Dimethylformamide Embryo Ethanol Glycerin HEPES Isopropyl Alcohol Larva Methanol methylethyl ketone polyethylene glycol 400 Propylene Glycol Sodium Chloride Solvents Strains Sulfate, Magnesium Sulfoxide, Dimethyl Zebrafish
5
Desiccation Kinetics and Rehydration of Interfilum Strains
For the desiccation experiments, a new standardized set-up was developed to follow the kinetics of controlled dehydration and subsequent rehydration on the effective quantum yield of photosystem II (PSII) using noninvasive pulse amplitude modulation (PAM) fluorometry. All PAM measurements were done on low-light acclimated samples (35–40 μmol photons · m−2 · s−1). In addition, the effect of increasing temperatures on photosynthesis and respiration was recorded using an oxygen optode.
Cells of each Interfilum strain were concentrated on four replicate Whatman GF/F glass fiber filters (Whatman, Dassel, Germany). Onto each filter, exactly 200 μL of the cell suspension (∼1–2 mg chl a · L−1; parallel filters for chl a concentration were determined using dimethyl formamide [DMF] as described below) was concentrated in the center as a light green spot using an Eppendorf Pipette. These moist filters were positioned on perforated metal grids (hole diameter: 1 mm; distance between holes: 1.5 mm) on top of four glass columns inside a transparent 200 mL polystyrol box, which was filled with 100 g of freshly activated silica gel (Silica Gel Orange, Carl Roth, Karlsruhe, Germany) and sealed with a transparent top lid (Fig.1). To record the relative air humidity (RAH) conditions inside the chambers, a PCE-MSR145S-TH mini data logger for air humidity and temperature was employed (PCE Instruments, Meschede, Germany; Fig.1). The boxes were kept under ambient room temperatures at 22°C ± 1°C and 40 μmol photons · m−2 · s−1 PAR (Osram light sources see above).
The effective quantum yield (ΔF/Fm') of PSII was regularly determined during the dehydration period (350–470 min depending on the strain) using a pulse amplitude modulated fluorimeter (PAM 2500; Heinz Walz GmbH, Effeltrich, Germany) according to the approach of Genty et al. (1989) . was calculated as with F as the fluorescence yield of light-treated algal cells (40 μmol photons · m−2 · s−1) and as the maximum light-adapted fluorescence yield after employing a 800 ms saturation pulse as described by Schreiber and Bilger (1993) . The PAM light probe was positioned outside the cover lid of the boxes (always 2 mm distance) to guarantee undisturbed RAH conditions inside, i.e., all fluorescence measurements were done through the polystyrol lids (Fig.1). The distance from the PAM light probe to the algal sample onto the glass fiber filters was always kept constant at 10 mm.
After the dehydration period, the dried glass fiber filters were transferred to a new polystyrol box which was filled with 100 mL tap water instead of silica gel to create a high humidity atmosphere (>95%). The filters were rehydrated by adding 200 μL of the standard growth medium to each filter and recovery of was followed with the same methodology as described (i.e., at 22°C ± 1°C and 40 μmol photons · m−2 · s−1).
Karsten U., Herburger K, & Holzinger A. (2014). Dehydration, temperature, and light tolerance in members of the aeroterrestrial green algal genus Interfilum (Streptophyta) from biogeographically different temperate soils. Journal of Phycology, 50(5), 804-816.
Publication 2014
Atmosphere Cell Respiration Cells Cultured Cells Culture Media Dehydration Desiccation Dimethylformamide DNA Replication Fluorescence Fluorometry Humidity Kinetics Light Metals Methyl Green Oxygen Photosynthesis Photosystem II Polystyrenes Pulse Rate Rehydration Silica Gel Strains Training Programs

Most recents protocols related to «Dimethylformamide»

1
Synthesis of Oxadiazole-Piperidine Compound
Not available on PMC !

Example 30

[Figure (not displayed)]

To a stirred solution of 3-(3,4-dimethoxyphenyl)-5-(4-piperidyl)-1,2,4-oxadiazole (150 mg, 518 μmol) in N,N-dimethylformamide (1.50 mL) were added (2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate) (196 mg, 518 μmol), N-ethyl-N-(propan-2-yl)propan-2-amine (201 mg, 1.56 mmol, 271 μL), and 2-(benzylamino)acetic acid (89 mg, 544 μmol). The mixture was stirred at 20° C. for 16 h and filtered, and the crude filtrate was purified directly by prep-HPLC (column: Luna C8 100×30 5 μm; mobile phase: [water (10 mM ammonium carbonate)-acetonitrile]; B%: 30%-60%, 12 min) to give 2-(benzylamino)-1-[4-[3-(3,4-dimethoxyphenyl)-1,2,4-oxadiazol-5-yl]-1-piperidyl]ethanone (48 mg, 110 μmol, 21%) as a yellow solid. 1H NMR (400 MHz, METHANOL-d4) δ=7.65 (dd, J=1.8, 8.2 Hz, 1H), 7.57 (d, J=1.8 Hz, 1H), 7.40-7.30 (m, 4H), 7.28-7.22 (m, 1H), 7.06 (d, J=8.4 Hz, 1H), 4.45 (br d, J=13.7 Hz, 1H), 3.94-3.83 (m, 7H), 3.78 (s, 2H), 3.57-3.44 (m, 2H), 3.40-3.33 (m, 1H), 3.27-3.20 (m, 1H), 3.01 (t, J=11.2 Hz, 1H), 2.17 (dd, J=2.8, 13.3 Hz, 2H), 1.93-1.73 (m, 2H); LCMS (ESI) m/z: [M+H]+=437.3.

US11873298B2. Compounds and uses thereof (2024-01-16). JANSSEN PHARMACEUTICA NV [BE]. Inventors: Matthew Lucas [US], Bertrand Le Bourdonnec [US], Iwona Wrona [US], Bhaumik Pandya [US], Parcharee Tivitmahaisoon [US], Kerem Ozboya [US], Benjamin Vincent [US], Daniel Tardiff [US], Jeff Piotrowski [US], Eric Solis [US], Robert Scannevin [US], Chee-Yeun Chung [US], Rebecca Aron [US], Kenneth Rhodes [US].
Full text: Click here
Patent 2024
1H NMR Acetic Acid acetonitrile Amines ammonium carbonate Dimethylformamide High-Performance Liquid Chromatographies Lincomycin Methanol Oxadiazoles
2
Programmable Nanocomposite Tectons for Ordered Assembly
Not available on PMC !

Example 1

This example describes an exemplary nanostructure (i.e. nanocomposite tecton) and formation of a material using the nanostructure.

A nanocomposite tecton consists of a nanoparticle grafted with polymer chains that terminate in functional groups capable of supramolecular binding, where supramolecular interactions between polymers grafted to different particles enable programmable bonding that drives particle assembly (FIG. 4). Importantly, these interactions can be manipulated separately from the structure of the organic or inorganic components of the nanocomposite tecton, allowing for independent control over the chemical composition and spatial organization of all phases in the nanocomposite via a single design concept. Functionalized polystyrene polymers were made from diaminopyridine or thymine modified initiators via atom transfer radical polymerization, followed by post-functionalization to install a thiol group that allowed for particle attachment (FIG. 5). The polymers synthesized had three different molecular weights (˜3.7, ˜6.0, and ˜11.0 kDa), as shown in FIG. 6, with narrow dispersity (Ð<1.10), and were grafted to nanoparticles of different diameters (10, 15, 20, and nm) via a “grafting-to” approach.

Once synthesized, nanocomposite tectons were functionalized with either diaminopyridine-polystyrene or thymine-polystyrene were readily dispersed in common organic solvents such as tetrahydrofuran, chloroform, toluene, and N,N′-dimethylformamide with a typical plasmonic resonance extinction peak at 530-540 nm (FIG. 7A) that confirmed their stability in these different solvents. Upon mixing, diaminopyridine-polystyrene and thymine-polystyrene coated particles rapidly assembled and precipitated from solution, resulting in noticeable red-shifting, diminishing, and broadening of the extinction peak within 1-2 minutes (example with 20 nm gold nanoparticles and 11.0 kDa polymers, FIG. 7B). Within 20 minutes, the dispersion appeared nearly colorless, and large, purple aggregates were visible at the bottom of the tube. After moderate heating (˜55° C. for ˜1-2 minutes for the example in FIG. 7B), the nanoparticles redispersed and the original color intensity was regained, demonstrating the dynamicity and complete reversibility of the diaminopyridine-thymine directed assembly process. Nanocomposite tectons were taken through multiple heating and cooling cycles without any alteration to assembly behavior or optical properties, signifying that they remained stable at each of these thermal conditions (FIG. 7C).

A key feature of the nanocomposite tectons is that the sizes of their particle and polymer components can be easily modified independent of the supramolecular binding group's molecular structure. However, because this assembly process is driven via the collective interaction of multiple diaminopyridine and thymine-terminated polymer chains, alterations that affect the absolute number and relative density of diaminopyridine or thymine groups on the nanocomposite tecton surface impact the net thermodynamic stability of the assemblies. In other words, while all constructs should be thermally reversible, the temperature range over which particle assembly and disassembly occurs should be affected by these variables. To better understand how differences in nanocomposite tecton composition impact the assembly process, nanostuctures were synthesized using different nanoparticle core diameters (10-40 nm) and polymer spacer molecular weights (3.7-11.0 kDa), and allowed to fully assemble at room temperature (˜22° C.) (FIG. 8). Nanocomposite tectons were then monitored using UV-Vis spectroscopy at 520 nm while slowly heating at a rate of 0.25° C./min, resulting in a curve that clearly shows a characteristic disassembly temperature (melting temperature, Tm) for each nanocomposite tecton composition.

From these data, two clear trends can be observed. First, when holding polymer molecular weight constant, Tm increases with increasing particle size (FIG. 8A). Conversely, when keeping particle diameter constant, Tm drastically decreases with increasing polymer length (FIG. 8B). To understand these trends, it is important to note that nanocomposite tecton dissociation is governed by a collective and dynamic dissociation of multiple individual diaminopyridine-thymine bonds, which reside at the periphery of the polymer-grafted nanoparticles. The enthalpic component of nanocomposite tecton bonding behavior is therefore predominantly governed by the local concentration of the supramolecular bond-forming diaminopyridine and thymine groups, while the entropic component is dictated by differences in polymer configuration in the bound versus unbound states.

All nanocomposite tectons possess similar polymer grafting densities (i.e. equivalent areal density of polymer chains at the inorganic nanoparticle surface, FIG. 9) regardless of particle size or polymer length. However, the areal density of diaminopyridine and thymine groups at the periphery of the nanocomposite tectons is not constant as a function of these two variables due to nanocomposite tecton geometry. When increasing inorganic particle diameter, the decreased surface curvature of the larger particle core forces the polymer chains into a tighter packing configuration, resulting in an increased areal density of diaminopyridine and thymine groups at the nanocomposite tecton periphery; this increased concentration of binding groups therefore results in an increased Tm, explaining the trend in FIG. 8A.

Conversely, for a fixed inorganic particle diameter (and thus constant number of polymer chains per particle), increasing polymer length decreases the areal density of diaminopyridine and thymine groups at the nanocomposite tecton periphery due to the “splaying” of polymers as they extend off of the particle surface, thereby decreasing Tm in a manner consistent with the trend in FIG. 8B. Additionally, increasing polymer length results in a greater decrease of system entropy upon nanocomposite tecton assembly, due to the greater reduction of polymer configurations once the polymer chains are linked via a diaminopyridine-thymine bond; this would also be predicted to reduce T m. Within the temperature range tested, all samples were easily assembled and disassembled via alterations in temperature. Inorganic particle diameter and polymer length are therefore both effective handles to control nanocomposite tecton assembly behavior.

Importantly, because the nanocomposite tecton assembly process is based on dynamic, reversible supramolecular binding, it should be possible to drive the system to an ordered equilibrium state where the maximum number of binding events can occur. The particle cores and polymer ligands are polydisperse (FIG. 10) and ordered arrangements represent the thermodynamically favored state for a set of assembled nanocomposite tectons. When packing nanocomposite tectons into an ordered lattice, deviations in particle diameter would be expected to generate inconsistent particle spacings that would decrease the overall stability of the assembled structure. However, the inherent flexibility of the polymer chains should allow the nanocomposite tectons to adopt a conformation that compensates for these structural defects. As a result, an ordered nanocomposite tecton arrangement would still be predicted to be stable if it produced a larger number of diaminopyridine-thymine binding events than a disordered structure and this increase in binding events outweighed the entropic penalty of reduction in polymer chain configurations.

To test this hypothesis, multiple sets of assembled nanocomposite tectons were thermally annealed at a temperature just below their Tm, allowing particles to reorganize via a series of binding and unbinding events until they reached the thermodynamically most stable conformation. The resulting structures were analyzed with small angle X-ray scattering, revealing the formation of highly ordered mesoscale structures where the nanoparticles were arranged in body-centered cubic superlattices (FIG. 11). The body-centered cubic structure was observed for multiple combinations of particle size and polymer length, indicating that the nanoscopic structure of the composites can be controlled as a function of either the organic component (via polymer length), the inorganic component (via particle size), or both, making this nanocomposite tecton scheme a highly tailorable method for the design of future nanocomposites.

US11873396B2. Nanostructures for the assembly of materials (2024-01-16). Massachusetts Institute of Technology [US]. Inventors: Robert J. Macfarlane [US], Jianyuan Zhang [US], Peter Jeffries Santos [US], Paul Anthony Gabrys [US].
Full text: Click here
Patent 2024
chemical composition Chloroform Cuboid Bone Dimethylformamide Entropy Extinction, Psychological Gold Human Body Ligands Molecular Structure Polymerization Polymers Polystyrenes Radiography Solvents Spectrum Analysis Sulfhydryl Compounds tetrahydrofuran Thymine Toluene Vibration Vision
3
Synthesis of N-[2-[4-[3-(3,4-dimethoxyphenyl)-1,2,4-oxadiazol-5-yl]-1-piperidyl]-2-oxo-ethyl]-N-methyl-benzamide
Not available on PMC !

Example 22

[Figure (not displayed)]

To a stirred solution of 3-(3,4-dimethoxyphenyl)-5-(4-piperidyl)-1,2,4-oxadiazole (150 mg, 518 μmol) in N,N-dimethylformamide (2 mL) was added (2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate) (196 mg, 518 μmol) and N-ethyl-N-(propan-2-yl)propan-2-amine (201 mg, 1.56 mmol, 271 μL) and 2-[benzoyl(methyl)amino]acetic acid (105 mg, 544 μmol). The mixture was stirred at 20° C. for 5 h, then cooled and purified directly by prep-HPLC (column: Luna C8 100×30 5 μm; mobile phase: [water (10 mM ammonium carbonate)-acetonitrile]; B%: 30%-60%, 12 min) to give N-[2-[4-[3-(3,4-dimethoxyphenyl) -1,2,4-oxadiazol-5-yl]-1-piperidyl]-2-oxo-ethyl]-N-methyl-benzamide (133 mg, 282 μmol, 54%) as a white solid. 1H NMR (400 MHz, DMSO-d6) δ=7.59 (dd, J=1.8, 8.4 Hz, 1H), 7.49-7.32 (m, 5H), 7.27 (br d, J=6.8 Hz, 1H), 7.16-7.08 (m, 1H), 4.44-4.24 (m, 2H), 4.21-4.03 (m, 1H), 4.02-3.88 (m, 1H), 3.88-3.74 (m, 6H), 3.56 (br d, J=13.7 Hz, 1H), 3.48-3.33 (m, 1H), 3.11-2.77 (m, 5H), 2.20-1.99 (m, 2H), 1.86 (br t, J=12.6 Hz, 1H), 1.74-1.48 (m, 2H), 1.43-1.26 (m, 1H); LCMS (ESI) m/z: [M+H]+=465.3.

US11873298B2. Compounds and uses thereof (2024-01-16). JANSSEN PHARMACEUTICA NV [BE]. Inventors: Matthew Lucas [US], Bertrand Le Bourdonnec [US], Iwona Wrona [US], Bhaumik Pandya [US], Parcharee Tivitmahaisoon [US], Kerem Ozboya [US], Benjamin Vincent [US], Daniel Tardiff [US], Jeff Piotrowski [US], Eric Solis [US], Robert Scannevin [US], Chee-Yeun Chung [US], Rebecca Aron [US], Kenneth Rhodes [US].
Full text: Click here
Patent 2024
1H NMR Acetic Acid acetonitrile Amines ammonium carbonate benzamide Dimethylformamide High-Performance Liquid Chromatographies Lincomycin N-methylbenzamide Oxadiazoles Sulfoxide, Dimethyl
4
Synthesis of 3-(Difluoromethyl)-4-iodo-1-methyl-1H-pyrazole
Not available on PMC !

Example 9

3-(Difluoromethyl)-4-iodo-1-methyl-1H-pyrazole (50 g) was added to a mixture of potassium carbonate (52.6 g), copper iodide (3.56 g) and sulfur powder (18.25 g) in dimethylformamide (DMF, 400 g). The reaction mixture was heated to a temperature of 110° C. and stirred for 3 to 4 hours at the same temperature. The progress of the reaction was monitored by Gas chromatography (GC). The reaction mixture was concentrated at a temperature of about 80° C. using reduced pressure of about 50 mbar to obtain a residue. Dichloromethane (150 ml) was added to the residue and filtered through hyflo super cell. The filter cake was washed with dichloromethane (150 ml). Organic layer was washed with water (500 ml×2). The organic layer was concentrated to obtain the desired product. GCMS 326+

Example 10

3-(Difluoromethyl)-4-iodo-1-methyl-1H-pyrazole (50 g) was added to a mixture of potassium carbonate (52.6 g), copper iodide (3.56 g) and sulfur powder (18.25 g) in sulfolane (400 g). The reaction mixture was proceeded as per example 9. GCMS 326+

US11873289B2. Pyrazole compounds and preparation thereof (2024-01-16). MYOKARDIA, INC. [US]. Inventors: Sunil Sharma [IN], Dinesh Jangid [IN], Priyanka Dhaka [IN], Siddharth Madhwal [IN], Bharat Kumar [IN], Kapil Kumar [IN], Rajdeep Anand [IN], Anurag Jain [IN].
Full text: Click here
Patent 2024
4-iodopyrazole Copper Dimethylformamide Gas Chromatography Gas Chromatography-Mass Spectrometry Iodides Methylene Chloride potassium carbonate Powder Pressure Pyrazoles sulfolane Sulfur
5
Synthesis of Oxadiazole-Carboxamide Compound
Not available on PMC !

Example 37

[Figure (not displayed)]

To a solution of 3-tert-butyl-1,2,4-oxadiazole-5-carboxylic acid (1.00 mg, 0.60 mmol) and N,N,N′,N′-tetramethyl-O-(7-azabenzotriazol-1-yl)uronium hexafluorophosphate (273 mg, 0.72 mmol) and 4-(5-amino-6,7,8,9-tetrahydro-5H-benzo[7]annulen-2-yl)-N-(1-methyl-1H-pyrazol-4-yl)pyrimidin-2-amine (200 mg, 0.6 mmol) in N,N-dimethylformamide (2.3 mL) was added N,N-diisopropylethylamine (0.42 mL, 2.4 mmol). The reaction was stirred at rt overnight and was quenched with MeOH. After workup, prep HPLC gave 3-(tert-butyl)-N-(2-(2-((1-methyl-1H-pyrazol-4-yl)amino)pyrimidin-4-yl)-6,7,8,9-tetrahydro-5H-benzo[7]annulen-5-yl)-1,2,4-oxadiazole-5-carboxamide as a solid (186 mg; yield: 64%). LCMS: Rt 1.38 min.; m/z 487.1; 1H NMR (400 MHz, METHANOL-d4) δ: 8.29 (br. s., 1H), 7.97-8.09 (m, 2H), 7.94 (s, 1H), 7.68 (br. s., 1H), 7.43 (d, J=8.78 Hz, 2H), 5.41 (d, J=9.79 Hz, 1H), 3.92 (s, 3H), 2.87-3.18 (m, 2H), 1.70-2.29 (m, 5H), 1.45 (s, 10H).

US11858926B2. Inhibiting agents for bruton's tyrosine kinase (2024-01-02). BIOGEN MA INC. [US]. Inventors: Brian T. Hopkins [US], Bin Ma [US], Robin Prince [US], Isaac Marx [US], Joseph P. Lyssikatos [US], Fengmei Zheng [US], Matthew Peterson [US], Daniel B. Patience [US].
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Patent 2024
1H NMR Amines Carboxylic Acids Dimethylformamide High-Performance Liquid Chromatographies Lincomycin Methanol Oxadiazoles pyrazole TERT protein, human

Top products related to «Dimethylformamide»

1
N n dimethylformamide (dmf) by Merck Group
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N,N-dimethylformamide is a clear, colorless liquid organic compound with the chemical formula (CH3)2NC(O)H. It is a common laboratory solvent used in various chemical reactions and processes.
2
Dimethylformamide by Merck Group
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Dimethylformamide is a colorless, hygroscopic, and highly polar organic solvent. It is commonly used as a laboratory reagent and in various industrial applications.
3
Dimethyl sulfoxide (dmso) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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N n dimethylformamide (dmf) by Thermo Fisher Scientific
Sourced in United States, Belgium, Germany, United Kingdom, China, Canada, Australia, France, India
N,N-dimethylformamide is a colorless, miscible liquid used as a solvent in various laboratory applications. It has a high boiling point and is widely employed in chemical synthesis, extraction processes, and analytical techniques.
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Methanol by Merck Group
Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan
Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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Ethanol by Merck Group
Sourced in Germany, United States, United Kingdom, Italy, India, France, China, Australia, Spain, Canada, Switzerland, Japan, Brazil, Poland, Sao Tome and Principe, Singapore, Chile, Malaysia, Belgium, Macao, Mexico, Ireland, Sweden, Indonesia, Pakistan, Romania, Czechia, Denmark, Hungary, Egypt, Israel, Portugal, Taiwan, Province of China, Austria, Thailand
Ethanol is a clear, colorless liquid chemical compound commonly used in laboratory settings. It is a key component in various scientific applications, serving as a solvent, disinfectant, and fuel source. Ethanol has a molecular formula of C2H6O and a range of industrial and research uses.
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N n dimethylformamide (dmf) by Sinopharm
Sourced in China, Argentina, United States
N,N-dimethylformamide is a colorless, hygroscopic liquid with a high boiling point. It is commonly used as a solvent in various chemical processes and laboratory applications.
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Acetonitrile by Merck Group
Sourced in Germany, United States, Italy, India, China, United Kingdom, France, Poland, Spain, Switzerland, Australia, Canada, Brazil, Sao Tome and Principe, Ireland, Belgium, Macao, Japan, Singapore, Mexico, Austria, Czechia, Bulgaria, Hungary, Egypt, Denmark, Chile, Malaysia, Israel, Croatia, Portugal, New Zealand, Romania, Norway, Sweden, Indonesia
Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.
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Dimethylformamide dmf by Merck Group
Sourced in United States, Germany, India, Australia, United Kingdom, Czechia, Spain, Italy, France
Dimethylformamide (DMF) is a colorless, volatile, and flammable liquid compound commonly used as a solvent in various industrial and laboratory applications. It has the chemical formula C₃H₇NO. DMF is known for its high solubility and its ability to dissolve a wide range of organic and inorganic substances.
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Sodium hydroxide (naoh) by Merck Group
Sourced in Germany, United States, India, United Kingdom, Italy, China, Spain, France, Australia, Canada, Poland, Switzerland, Singapore, Belgium, Sao Tome and Principe, Ireland, Sweden, Brazil, Israel, Mexico, Macao, Chile, Japan, Hungary, Malaysia, Denmark, Portugal, Indonesia, Netherlands, Czechia, Finland, Austria, Romania, Pakistan, Cameroon, Egypt, Greece, Bulgaria, Norway, Colombia, New Zealand, Lithuania
Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.

More about "Dimethylformamide"

Dimethylformamide (DMF) is a versatile organic solvent that is widely used in chemical synthesis, pharmaceutical research, and material science.
It is a colorless, flammable liquid with a distinctive fishy odor.
DMF has a high boiling point and is miscible with water, making it a useful solvent for a variety of organic and inorganic compounds.
Researchers can use PubCompare.ai's AI-driven platform to optimize their DMF research by easily locating protocols from literature, preprints, and patents, and using AI-driven comparisons to identify the best protocols and products for their experiments.
This can help streamline research and save time.
DMF is similar to other common organic solvents like N,N-dimethylformamide, DMSO, Methanol, Ethanol, and Acetonitrile.
These solvents have different properties and applications, and researchers may need to use a combination of them depending on the specific requirements of their experiments.
When working with DMF, it's important to take proper safety precautions, as it can be toxic and flammable.
Researchers should always follow safety protocols and use appropriate personal protective equipment (PPE) when handling DMF.
By utilizing the insights and tools provided by PubCompare.ai, researchers can streamline their DMF research, save time, and optimize their experiments for better results.
This can lead to advancements in various fields, including chemistry, materials science, and pharmaceutical development.