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Dorsomorphin

Dorsomorphin is a small molecule inhibitor that selectively targets the bone morphogenetic protein (BMP) signaling pathway.
It has been widely used in research to investigate the role of BMP signaling in various biological processes, including embryonic development, stem cell differentiation, and tissue regeneration.
Dorsomorphin inhibits the activity of BMP type I receptors, such as BMPR1A and BMPR1B, thereby blocking the downstream signaling cascades that regulate gene expression and cellular responses.
This tool has become an indispensable resource for researchers studying the Dorsomorphin pathway and its implications in health and disease.
PubCompare.ai offers a streamlined, AI-powered platform to help scientists efficiently locate, compare, and optimize Dorsomorphin research protocols from the literature, preprints, and patents, empowering them to conduct more reproducible and impactful experiments.
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Most cited protocols related to «Dorsomorphin»

1
Optimizing Cardiac Differentiation of Pluripotent Stem Cells
Both hiPSCs and hESCs (>p20) were split at 1:10 or 1:12 ratios, using EDTA as above and grown for 4 days, at which time they reached ~85% confluence. Medium was changed to CDM3, consisting of RPMI 1640 (11875, Life Technologies), 500 µg/mL Oryza sativa-derived recombinant human albumin (A0237, Sigma-Aldrich, 75 mg/mL stock solution in WFI H2O, stored at −20 °C), and 213 µg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich, 64 mg/mL stock solution in WFI H2O, stored at −20 °C). Medium was changed every other day (48 h). For d0-d2, medium was supplemented with 6 µM CHIR99021 (LC Laboratories). On d2, medium was changed to CDM3 supplemented with 2 µM Wnt-C59 (Selleck Chemicals). Medium was changed on d4 and every other day for CDM3. Contracting cells were noted from d7.
Other additions to cardiac differentiation media tested were 10.7 µg/mL recombinant human transferrin, 14 µg/mL sodium selenite, 1 µg/mL linoleic acid, 1 µg/mL linolenic acid, 2 ng/mL triiodo-l-thyronine, 2 µg/mL L-carnitine, 1 µg/mL D,L-alpha-tocopherol acetate, 100 ng/mL retinol acetate, 1 µg/mL ethanolamine, 20 ng/mL corticosterone, 9 ng/mL progesterone, 47 ng/mL lipoic acid, 100 ng/mL retinol, 1 µg/mL D,L-alpha-tocopherol, 100 ng/mL biotin, 2.5 ug/mL catalase, 2.5 µg/mL glutathione, 2.5 µg/mL superoxide dismutase, 2 µg/mL L-carnitine, 15 µg/mL D(+)-galactose, 16.1 µg/mL putrescine, 450 µM 1-thioglycerol, 55 µM 2-mercaptoethanol, and 64 µg/mL L-ascorbic acid 2-phosphate (all from Sigma-Aldrich).
Basal media assessed were DMEM (catalogue #11965), DMEM/F12 (11330), IMDM (12440), IMDM/F12 (12440/11765), RPMI 1640 (11875), McCoy’s 5A (16600), M199 (with Earle’s Salts, 11150), MEMα (with Earle’s Salts, no nucleosides, 12561), and MEM (with Earle’s Salts, 11095) (all from Life Technologies). RPMI 1640 media assessed were RPMI 1640 with L-glutamine (catalogue number 11875), RPMI 1640 with L-glutamine and HEPES (22400), RPMI 1640 with GlutaMAX (61870), and RPMI with GlutaMAX and HEPES (72400) (all from Life Technologies).
Albumin sources assessed were human serum albumin (A1653, Sigma-Aldrich), Oryza sativa-derived recombinant human albumin (A0237, Sigma-Aldrich), Saccharomyces cerevisiae-derived recombinant Albucult (Novozymes Biopahrma/A6608, Sigma Aldrich), Oryza sativa-derived recombinant Cellastim (Invitria/A9731, Sigma Aldrich), and embryo-grade bovine serum albumin (A3311, Sigma Aldrich).
Wnt inhibitors assessed were IWP-2, IWR-1 (both Sigma-Aldrich), XAV-939, ICG-001 (Selleck Chemicals), IWP-4 (Stemgent), and Wnt-C59 (Selleck Chemicals). GSK3B inhibitors assessed were CHIR99021 (LC Laboratories), BIO, TWS119 (Selleck Chemicals), 1-azenkenpaullone, TDZD-8, ARA014418, and 3F8 (all Sigma-Aldrich). Inhibitors used for pathway analysis were PD173074, SB203580, LDN193189, SB431542 (all Selleck Chemicals), SU5402, Dorsomorphin, A83-01 (all Tocris), ALK5 inhibitor (Stemgent), and ITD-1 (Xcessbio). All small molecules were resuspended to 10 mM in dimethyl sulfoxide (DMSO) and used at 5 µM except for Wnt-C59 which was used at 2 µM.
For control treatments (0 µM) 0.1% DMSO was used. Comparisons of differentiation media were made to RPMI+B27-ins consisting of RPMI 1640 (11875) supplemented with 2% B27 without insulin (0050129SA, Life Technologies), and StemPro-34 (Life Technologies) supplemented as shown in Supplementary Table 1. LI-APEL low insulin medium and Xeno-free Differentiation Medium were made as described2 (link), 9 (link), 48 (link). For optimization of cardiac differentiation conditions, cells were differentiated in 12-well plates and samples were analyzed at day 15 of differentiation after dissociation with TrypLE Express for 10 min at 37 °C.
Burridge P.W., Matsa E., Shukla P., Lin Z.C., Churko J.M., Ebert A.D., Lan F., Diecke S., Huber B., Mordwinkin N.M., Plews J.R., Abilez O.J., Cui B., Gold J.D, & Wu J.C. (2014). Chemically Defined and Small Molecule-Based Generation of Human Cardiomyocytes. Nature methods, 11(8), 855-860.
Publication 2014
2
Neural Induction and Differentiation of hESCs/iPSCs
hESCs or iPSCs were isolated from MEFs following dissociation to single cells with Accutase (Innovative Cell Technologies) by a 1 hr pre-plate on gelatin-coated dishes in hESC medium supplemented with 10 ng/ml FGF2 and 10 μM ROCK inhibitor (Calbiochem). The non-adherent pluripotent stem cells were harvested and plated on Matrigel (BD) coated 12-well plates in MEF-conditioned hESC medium with 10 ng/ml FGF2. Once the cell culture reached 95% confluence, neural induction was initiated by changing the culture medium to a culture medium that supports neural induction, neurogenesis and neuronal differentiation (referred to as 3N medium), a 1:1 mixture of N2- and B27-containing media. N2 medium: DMEM/F12, N2 (GIBCO), 5 μg/ml Insulin, 1mM L-Glutamine, 100 μm non-essential amino acids, 100 μM 2-mercaptoethanol, 50 U/ml Penicillin and 50 mg/ml Streptomycin; B27 medium: Neurobasal (Invitrogen), B27 with or without vitamin A (GIBCO), 200 mM Glutamine, 50 U/ml Penicillin and 50 mg/ml Streptomycin. 3N medium was supplemented with either 1 μm Dorsomorphin (Tocris) or 500 ng/ml mouse Noggin-CF chimera (R&D Systems), and 10 μm SB431542 (Tocris) to inhibit TGFβ signaling during neural induction 19 (link). Cells were maintained in this medium for 8-11 days, during which time the efficiency of neural induction was monitored by the appearance of cells with characteristic neuroepithelial cell morphology. Neuroepithelial cells were harvested by dissociation with Dispase and replated in 3N medium including 20 ng/ml FGF2 on poly-ornithine and laminin-coated plastic plates. After a further 2 days, FGF2 was withdrawn to promote differentiation. Cultures were passaged once more with Accutase, replated at 50,000 cells/cm2 on poly-ornithine and laminin-coated plastic plates in 3N medium and maintained for up to 100 days with a medium change every other day.
For quantitative RT-PCR, total RNA was isolated from three cultures at each timepoint (days 5, 10, 15, 20 and 25) (Trizol, Sigma). Total RNA was reverse-transcribed and used for quantitative RT-PCR with primers specific to Foxg1 and Tbr2 using the Applied Biosystems 7000 system. Semi-quantitative RT-PCR with primers for Emx1, Dlx1, Nkx2.1, HoxB4 and Isl1 was carried out according to standard techniques on first strand, random-primed cDNA generated from total RNA extracted from cultures grown in the presence or absence of purmorphamine.
Shi Y., Kirwan P., Smith J., Robinson H.P, & Livesey F.J. (2012). Human cerebral cortex development from pluripotent stem cells to functional excitatory synapses. Nature neuroscience, 15(3), 10.1038/nn.3041.
Publication 2012
2-Mercaptoethanol 4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide accutase Amino Acids, Essential Cardiac Arrest Cell Culture Techniques Cells Chimera Culture Media, Conditioned dispase DNA, Complementary dorsomorphin Fibroblast Growth Factor 2 Gelatins Glutamine Human Embryonic Stem Cells Hyperostosis, Diffuse Idiopathic Skeletal Induced Pluripotent Stem Cells Insulin Laminin matrigel Mus Nervousness Neuroepithelial Cells Neurogenesis Neurons NKX2-1 protein, human noggin protein Oligonucleotide Primers Ornithine Penicillins Pluripotent Stem Cells Poly A purmorphamine Reverse Transcriptase Polymerase Chain Reaction Streptomycin Transforming Growth Factor beta trizol Vitamin A
3
Neural Induction of Pluripotent Stem Cells
For dissociating intact colonies of pluripotent stem cells from the layer of DR4 feeders, hiPSCs were exposed to a low concentration of dispase (Invitrogen: 17105-041; 0.7 mg/ml) for ~30 min. Suspended colonies were subsequently transferred into ultra-low-attachment 100 mm plastic plates (Corning) in hiPSC medium without FGF2. For the first 24 h (day 0), the medium was supplemented with the ROCK inhibitor Y-27632 (EMD Chemicals). For neural induction, dorsomorphin (also known as compound C; Sigma 10 μM) and SB-431542 (Tocris, 10 μM) were added to the medium for the first five days. On the sixth day in suspension, the floating spheroids were moved to neural medium (NM) containing Neurobasal (Invitrogen: 10888), B-27 serum substitute without vitamin A (Invitrogen: 12587), GlutaMax (Invitrogen, 1:100), 100 U/ml penicillin and 100 μl streptomycin (Invitrogen). The NM was supplemented with 20 ng/ml FGF2 (R&D Systems) and 20 ng/ml EGF (R&D Systems) for 19 days with daily medium change in the first 10 days, and every other day for the subsequent 9 days. To promote differentiation of the neural progenitors into neurons, FGF2 and EGF were replaced with 20 ng/ml BDNF (Peprotech) and 20 ng/ml NT3 (Peprotech) starting at day 25, while from day 43 onwards only NM without growth factors was used for medium changes every four days.
Paşca A.M., Sloan S.A., Clarke L.E., Tian Y., Makinson C.D., Huber N., Kim C.H., Park J.Y., O’Rourke N.A., Nguyen K.D., Smith S.J., Huguenard J.R., Geschwind D.H., Barres B.A, & Paşca S.P. (2015). Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture. Nature methods, 12(7), 671-678.
Publication 2015
4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide dispase dorsomorphin Feeder Cell Layers Fibroblast Growth Factor 2 Growth Factor Human Induced Pluripotent Stem Cells Nervousness Neurons Penicillins Pluripotent Stem Cells Serum Streptomycin Vitamin A Y 27632
4
Differentiation of Human Neural Progenitors
The protocol is illustrated in Extended Data Fig. 2a. Specifically, iPS cell colonies were detached from the feeder layer with 1 mg ml−1 collagenase treatment for 1h and suspended in embryoid body (EB) medium, consisting of FGF-2-free iPS cell medium supplemented with 2 μM dorsomorphin and 2 μM A-83, in non-treated polystyrene plates for 4 days with a daily medium change. After 4 days, EB medium was replaced by neural induction medium (hNPC medium) consisting of DMEM/F12, N2 supplement, NEAA, 2mg ml−1 heparin and 2 μM cyclopamine. The floating EBs were then transferred to Matrigel-coated 6-well plates at day 7 to form neural tube-like rosettes. The attached rosettes were kept for 15days with hNPC medium change every other day. On day 22, the rosettes were picked mechanically and transferred to low attachment plates (Corning) in hNPC medium containing B27. For neuronal differentiation, resuspended neural progenitor spheres were dissociated with Accutase at 37°C for 10 min and placed onto poly-D-lysine/laminin-coated coverslips in the neuronal culture medium, consisting of Neurobasal medium supplemented with 2 mM L-glutamine, B27, 10 ng ml−1 BDNF and 10 ng ml−1 GDNF. Half of the medium was replaced once a week during continuous culturing. For electrophysiological recordings, neural progenitors were plated on a confluent layer of rodent astrocytes as previously described21 (link),34 (link). These cultures exhibited similar neuronal densities and parallel cultures were used for recordings of different iPS cell lines in a blind fashion.
Wen Z., Nguyen H.N., Guo Z., Lalli M.A., Wang X., Su Y., Kim N.S., Yoon K.J., Shin J., Zhang C., Makri G., Nauen D., Yu H., Guzman E., Chiang C.H., Yoritomo N., Kaibuchi K., Zou J., Christian K.M., Cheng L., Ross C.A., Margolis R.L., Chen G., Kosik K.S., Song H, & Ming G.L. (2014). Synaptic dysregulation in a human iPS cell model of mental disorders. Nature, 515(7527), 414-418.
Publication 2014
accutase Astrocytes Cell Lines Collagenase cyclopamine dorsomorphin Embryoid Bodies Feeder Cell Layers Fibroblast Growth Factor 2 Glial Cell Line-Derived Neurotrophic Factor Glutamine Heparin Induced Pluripotent Stem Cells Laminin Lysine matrigel Nervousness Neurons Poly A Polystyrenes Rodent Tube, Neural Visually Impaired Persons
5
Optimized Feeder-Free Culture of hPSCs
Validated HuES6 and NCL3 hESCs were from the Harvard Office of Technology Development and the UK Stem Cell Bank, respectively, and used below passage 50 [34] (link), [35] (link). hESCs and hiPSCs (see below) were exclusively grown under feeder-free conditions using 6 and 12-well tissue culture plates coated with 0.0125% (v/v) diluted Matrigel® HC (BD) [36] (link). The medium used as a starting point for optimization was N2B27 with FGF2 [14] (link). Products deviating from those described in [14] (link) were confirmed to be rather independent of the supplier: N2B27 contained DMEM/F12 (Invitrogen #21331-020, PAA #E15-012, or Hyclone #SH3012601), 1x N2 supplement (Invitrogen #17502-048 or PAA #F005-004), 1x B27 supplement (Invitrogen #12587-010), 0.05% BSA (Sigma #A1595), 2 mM L-glutamine (PAA #M11-004 - if not contained in DMEM/F12), 1x non-essential amino acids (PAA #M11-003), 0.1 mM β-mercaptoethanol (Invitrogen #31350-010), 1x penicillin/streptomycin (PAA #P11-010), and 20 ng/ml FGF2 (Peprotech #100-18B).
For splitting cells, hPSC colonies were cut into equally sized fragments using a sterile injection needle. After washing once with PBS, the cells were incubated with 2 mg/ml Dispase (Invitrogen #17105-041) for 5–10 min at 37°C and subsequently washed three times with PBS. Cells were then scraped off with a sharp cell scraper and transferred to a 15 ml conical tube. After brief centrifugation (5s at 200g), clumps were resuspended in medium supplemented with 10 µM Y27632 (Abcam #ab120129) and transferred to equilibrated medium in Matrigel®-coated culture dishes.
For stepwise systematic media optimization, cells were grown in the presence or absence of a given factor and monitored under these conditions over a period of at least 3 passages. Towards the end of a given passage, one half of the cells growing in wells of 6-well plates was scraped out and used for RNA isolation, followed by RT-qPCR analysis using panels of self-renewal and early differentiation markers. The other half of a given well was used for splitting and maintaining the cells under the same conditions, followed by repeating the procedure several days later etc. To account for cell line-dependent effects, most optimzation experiments were carried out using two independent hESC lines, HuES6 and NCL3. FTDA medium was compared to the following commercially available media: NutriStem® (Stemgent #01-0005-100), StemPro® (Invitrogen #A10007-01) and mTeSR®1 (Stemcell Technologies #05850). All media were used according to the manufacturers’ protocols. E8 medium was prepared as described in Chen et al. [22] (link), using 100 ng/ml of human FGF2 and 2 ng/ml TGFβ1. Sources of BSA were Sigma #A1595, Invitrogen #A10008-01, and Invitrogen #15260-037.
Optimized FTDA medium was composed of DMEM/F12 (Invitrogen #21331-020), 2 mM L-glutamine (PAA, as not contained in the DMEM/F12 used), 1x ITS (BD Biosciences #354351), 0.1% HSA (Biological Industries #05-720-1B), 1x chemically defined lipids (Invitrogen #11905-031, 1∶100), 5–10 ng/ml FGF2 (Peprotech #100-18B), 0.5 ng/ml TGFβ1 (Peprotech #100-21C), 2.5–5 ng/ml Activin A (R&D #1066-AB-005 or eBioscience #14-8993-62), 50 nM DM (Merck), and 1x penicillin/streptomycin (PAA). Line iPS 1 additionally received 2 µM IWP-2 (Merck). We noticed that Activin A showed marked differences in activity between different manufacturers. We therefore tested several products and, hence after, only used Activin A from R&D or eBioscience. For sustained undifferentiated growth in FTDA, we found it is crucial to feed cells on a daily basis.
For analyzing growth rates, HuES6 hESCs were plated as clumps into replicate wells containing either FTDA or MEF-CM [4] (link). Every 24 hours, cells from a given well were dissociated with Accutase® and counted. Quantification of colony sizes was done by determining relative colony diameters of at least 20 colonies from a given well and converting the values into relative areas occupied by the colonies. For clonal expansion, cells were split with Accutase, re-seeded at low density in the presence of 10 µM Y27632, and fed with FTDA hence after.
For differentiation into germ layer precursors, hESCs were replated as clumps or single cells in FTDA, followed by treatment with specific growth factors/pharmacological inhibitors, while leaving the other components of the medium unchanged: PAX6-postive neuroectoderm was induced by treating cells for 5 days with 0.5 µM PD0325901 (Axon Medchem), 15 µM SB431542 (Ascent), and 0.5 µM dorsomorphin (DM, Merck) [36] (link); MESP1-positive mesoderm was induced by treating cells for 2 days with 10 ng/ml FGF2, 5 ng/ml Activin A (eBioscience #14-8993-80), 10 ng/ml BMP4 (R&D), and 5 µM CHIR99021 (Axon Medchem); SOX17-positive endoderm was induced by treatment with 10 ng/ml FGF2, 100 ng/ml Activin A, 10 ng/ml BMP4, and 5 µM CHIR99021 for 1d, followed by 100 ng/ml Activin A for 3 days. For testing the effect of WNT activation in routine culture, CHIR99021 (Axon Medchem) was used at 1–3 µM. Human recombinant LIF (Millipore) was tested at concentrations of up to 50 ng/ml.
For terminal differentiation into neurons, cells growing in FTDA were harvested as for routine passaging, and embryoid bodies formed by plating cell aggregates into suspension culture dishes using basal defined medium (Table 1) with FGF2, DM, and Y27632. For the next 4 days, EBs were treated with 0.5 µM PD0325901, 15 µM SB431542, 0.5 µM DM, and Y27632, then plated out on Matrigel and treated with PD0325901, SB431542, and DM for another 4 days [36] (link). Beating cardiomyocytes were generated by plating aggregates of FTDA-adapted hESCs in basal defined medium without growth factors (Table 1) onto confluent feeder layers of cardiogenic END2 cells [37] (link). Medium was changed every 5 days. After 2–3 weeks, beating clusters were manually isolated, digested with trypsin, and replated onto gelatin-coated dishes. Staining for cardiac markers was performed several days later. AFP-positive fetal liver cells were generated by spontaneous EB differentiation: EBs were generated as above, from cells growing long-term in FTDA, and then transferred to DMEM/F12 with 20% FCS. EBs were plated out onto gelatin-coated dishes after 1 week, and stained for AFP 2 weeks later.
Frank S., Zhang M., Schöler H.R, & Greber B. (2012). Small Molecule-Assisted, Line-Independent Maintenance of Human Pluripotent Stem Cells in Defined Conditions. PLoS ONE, 7(7), e41958.
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Publication 2012

Most recents protocols related to «Dorsomorphin»

1
Structural Insights into ABCG2 Inhibition
Not available on PMC !
The human ABCG2 protein crystal structure was archived from the Protein Data Bank (PDB ID: 6vxi). The molecular dockings of dorsomorphin and ABCG2 were analyzed using AutoDock Vina, and the data were visualized using PyMOL.
Li X.P., Cao L.Q., Yu Z.Z., He K., Ding P.B., Li J.S., Shan Y.Y., Su Y.B., Yuan Z.M, & Shi Z. (2024). Dorsomorphin attenuates ABCG2-mediated multidrug resistance in colorectal cancer. Frontiers in pharmacology, 15.
Publication 2024
2
Cellular Drug Uptake Assay
Not available on PMC !
The cells were cultured in 12-well plates at 5 × 10 4 cells/well and incubated with the indicated concentration of dorsomorphin or KU55933 for 1 h. After incubating with mitoxantrone, doxorubicin, or rhodamine 123 at a concentration of 10 μM for another 2 h, the images of the cells were acquired using the LSM900 confocal microscope from Carl Zeiss Inc. (Oberkohen, Germany). Next, the cells were collected and analyzed with a CytoFLEX flow cytometer from Beckman Coulter Inc. (Brea, California, United States), as reported previously (Liu et al., 2022) .
Li X.P., Cao L.Q., Yu Z.Z., He K., Ding P.B., Li J.S., Shan Y.Y., Su Y.B., Yuan Z.M, & Shi Z. (2024). Dorsomorphin attenuates ABCG2-mediated multidrug resistance in colorectal cancer. Frontiers in pharmacology, 15.
Publication 2024
3
Colorectal Cancer ABCG2 Regulation Assay
Not available on PMC !
Dorsomorphin (#1219188-18-9), KU55933 (#587871-26-9), mitoxantrone (#70476-82-3), doxorubicin (#A603456-0025), cisplatin (#AA1A8019B), rhodamine 123 (#62669-70-9), and 3-(4,5-dimethylthiazol-yl)-2,5diphenyl-tetrazolium bromide (MTT; #298-93-1) were procured from MREDA Technology Inc. (Beijing, China), TargetMol Chemicals Inc. (Shanghai, China), D&B Biotech Inc. (Shanghai, China), Sangon Biotech Inc. (Shanghai, China), Qilu Pharmaceutical Co. (Jinan, China), Sigma-Aldrich Trading Co. (Shanghai, China), and Yuanye Biotech Co. (Shanghai, China), respectively. Anti-ABCG2 antibody (#RLT0053) and anti-β-actin antibody (#SC-47778) were purchased from Ruiying Biotech (Wuxi, China) and Santa Cruz Biotech (Santa Cruz, California, United States), respectively. Human colorectal cancer cells S1-M1-80 vector with ABCG2 overexpression and S1-M1-80 sgABCG2 with ABCG2-knockout were established as reported previously (Liu et al., 2021) and cultured in Dulbecco's modified Eagle's medium (#C11995500BT) with 10% fetal bovine serum (#10270-106) from Thermo Fisher Scientific Inc. (Waltham, Massachusetts, United States) at 37°C in a humid atmosphere of 5% CO 2 .
Li X.P., Cao L.Q., Yu Z.Z., He K., Ding P.B., Li J.S., Shan Y.Y., Su Y.B., Yuan Z.M, & Shi Z. (2024). Dorsomorphin attenuates ABCG2-mediated multidrug resistance in colorectal cancer. Frontiers in pharmacology, 15.
Publication 2024
4
Organoid Formation from Mouse ESCs
The protocol for organoid formation was based on the cortical differentiation protocol above. Briefly, 2000 cells were seeded per well of a round bottom, ultralow attachment, 96-well plate in mESC basal media with LIF, SB431542, and dorsomorphin. Forty-eight hours later, the spent medium was removed and replaced with NMM+ SB431542, dorsomorphin, I-BET151, and retinoic acid. The next day, this medium was removed, and NMM with SB431542 and Dorsomorphin was added. Forty-eight hours later, half the volume of the culture medium was replaced. At days 6 and 8, half of the medium was replaced with basal media (NMM). On day 10, the organoids were split evenly and 24 organoids were placed into 6-cm ultralow attachment dishes. On day 16, organoids were embedded in undiluted Matrigel (Corning, 356234, lot 229001) in the same medium and kept on a shaker at 16 rpm for at least 21 days.
Cortes D.E., Escudero M., Korgan A.C., Mitra A., Edwards A., Aydin S.C., Munger S.C., Charland K., Zhang Z.W., O'Connell K.M., Reinholdt L.G, & Pera M.F. (2024). An in vitro neurogenetics platform for precision disease modeling in the mouse. Science Advances, 10(14), eadj9305.
Publication 2024
5
AMPK and Notch Modulation in gADSC Adipogenesis
gADSCs were seeded and cultured in 6-well plates. When the cell confluence reached 50%, cells were treated with 0.2 mM AICAR (AMPK activator), 4.0 μM Dorsomorphin (Compound C) 2HCl (AMPK inhibitor), 0.4 mM Valproic acid (VPA) (NOTCH activator), and 1.2 μM LY411575 (NOTCH inhibitor) for 24 h. Untreated gADSCs and gADSCs treated with AICAR, Dorsomorphin (Compound C) 2HCl, Valproic acid (VPA), and LY411575 were simultaneously differentiated into adipocytes. The induction and identification were conducted using a method similar to that of the induction of gADSCs differentiation into adipocytes in this study.
Wang Z., Bi M., Zhe X., Wang X., Dai B., Han X., Ren B., Liang H, & Liu D. (2024). Molecular mechanism underlying miR-204-5p regulation of adipose-derived stem cells differentiation into cells from three germ layers. Cell Death Discovery, 10, 95.
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Publication 2024

Top products related to «Dorsomorphin»

1
Dorsomorphin by Merck Group
Sourced in United States, United Kingdom, Macao, Germany
Dorsomorphin is a synthetic compound that functions as a selective inhibitor of bone morphogenetic protein (BMP) signaling. It acts by blocking the activity of BMP type I receptors, which are involved in the regulation of various cellular processes. The core function of Dorsomorphin is to serve as a tool for studying BMP signaling pathways in biological research.
2
Dorsomorphin by Bio-Techne
Sourced in United Kingdom, United States
Dorsomorphin is a small molecule compound that functions as a selective inhibitor of the bone morphogenetic protein (BMP) signaling pathway. It acts by blocking the phosphorylation and activation of BMP-specific SMAD transcription factors.
3
Sb431542 by Bio-Techne
Sourced in United Kingdom, United States, Canada, China
SB431542 is a potent and selective inhibitor of the transforming growth factor-beta (TGF-β) type I receptor activin receptor-like kinase (ALK) 4, ALK5, and ALK7. It blocks TGF-β signaling by inhibiting the phosphorylation and activation of Smad2 and Smad3.
4
N2 supplement by Thermo Fisher Scientific
Sourced in United States, Germany, Japan, United Kingdom, France, Italy, China, Canada, Czechia, Belgium, Australia, Switzerland
The N2 supplement is a laboratory-grade nitrogen enrichment solution used to support the growth and development of cell cultures. It provides an additional source of nitrogen to cell culture media, which is essential for cellular metabolism and protein synthesis.
5
Glutamax by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, France, Switzerland, Canada, Japan, Australia, China, Belgium, Italy, Denmark, Spain, Austria, Netherlands, Sweden, Ireland, New Zealand, Israel, Gabon, India, Poland, Argentina, Macao, Finland, Hungary, Brazil, Slovenia, Sao Tome and Principe, Singapore, Holy See (Vatican City State)
GlutaMAX is a chemically defined, L-glutamine substitute for cell culture media. It is a stable source of L-glutamine that does not degrade over time like L-glutamine. GlutaMAX helps maintain consistent cell growth and performance in cell culture applications.
6
Dorsomorphin by Selleck Chemicals
Sourced in United States
Dorsomorphin is a small molecule compound used as a laboratory reagent. It functions as an inhibitor of bone morphogenetic protein (BMP) signaling pathways.
7
Dmem f12 by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Japan, Canada, Australia, Italy, Switzerland, Belgium, New Zealand, Spain, Denmark, Israel, Macao, Ireland, Netherlands, Austria, Hungary, Holy See (Vatican City State), Sweden, Brazil, Argentina, India, Poland, Morocco, Czechia
DMEM/F12 is a cell culture medium developed by Thermo Fisher Scientific. It is a balanced salt solution that provides nutrients and growth factors essential for the cultivation of a variety of cell types, including adherent and suspension cells. The medium is formulated to support the proliferation and maintenance of cells in vitro.
8
Sb431542 by Merck Group
Sourced in United States, Germany, United Kingdom, China, Japan, Australia, Sao Tome and Principe
SB431542 is a small molecule that inhibits the transforming growth factor-beta (TGF-β) signaling pathway. It acts as a selective and potent inhibitor of the activin receptor-like kinase (ALK) receptors, primarily ALK4, ALK5, and ALK7. This compound can be used in cell culture and research applications to investigate the role of the TGF-β signaling pathway.
9
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
10
B27 supplement by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Japan, Canada, China, Italy, France, Switzerland, Spain, Israel, Australia, Austria, Poland, Denmark, Palestine, State of
B27 supplement is a serum-free and animal component-free cell culture supplement developed by Thermo Fisher Scientific. It is designed to promote the growth and survival of diverse cell types, including neurons, embryonic stem cells, and other sensitive cell lines. The core function of B27 supplement is to provide a defined, optimized combination of vitamins, antioxidants, and other essential components to support cell culture applications.

More about "Dorsomorphin"

Dorsomorphin, also known as Compound C, is a small molecule inhibitor that selectively targets the bone morphogenetic protein (BMP) signaling pathway.
It has become a widely used tool in research to investigate the role of BMP signaling in various biological processes, such as embryonic development, stem cell differentiation, and tissue regeneration.
This compound inhibits the activity of BMP type I receptors, such as BMPR1A and BMPR1B, thereby blocking the downstream signaling cascades that regulate gene expression and cellular responses.
Researchers often combine Dorsomorphin with other key components to create effective cell culture systems.
For example, SB431542 is another small molecule inhibitor that can be used in conjunction with Dorsomorphin to inhibit the TGF-beta signaling pathway, which is important for regulating cell growth and differentiation.
N2 supplement and GlutaMAX are also commonly used in cell culture media to provide essential nutrients and support cell growth.
DMEM/F12 is a widely used basal medium, and fetal bovine serum (FBS) and B27 supplement can be added to provide additional growth factors and support for cell culture.
By utilizing a comprehensive understanding of Dorsomorphin and its interactions with these related components, researchers can design more effective and reproducible experiments to study the BMP signaling pathway and its implications in health and disease.
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