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EDTA, Disodium

EDTA, Disodium is a chelating agent commonly used in biochemical and biomedical research.
It functions by binding to metal ions, including calcium and magnesium, which are essential for various cellular processes.
Disodium EDTA is widely utilized in cell culture media, blood collection tubes, and other applications where the control of divalent cations is crucial.
Researchers rely on this compound to maintain the integrity and stability of biomolecules, cells, and tissues during experimentation.
Disodium EDTA's ability to chelate ions makes it a valuable tool for researchers seeking to optimize their experimental protocols and enhance the reproducibility of their findings.

Most cited protocols related to «EDTA, Disodium»

1
Benthic Meiofauna Sampling and DNA Extraction
A total of 24 benthic samples were collected from the low-tide mark along an 800 m transect, using a standard corer methodology46 , from marine sandy beach substrata in Prestwick (three every 100 m between 55°30.481′N, 4°37.489′W and 55°30.194′N, 4°37.368′W) and a further three from Littlehampton (50°48.021′N, 00°32.530′W), UK, during the summer of 2007. The latter sampling site was used as a geographically disparate out-group comparison. For the sequencing analysis, each biological sample comprised three pooled 44 mm diameter×100 mm benthic samples taken approximately 10 m apart. An additional core was taken for sediment analysis. All samples were immediately fixed in 500 ml storage pots containing 300 ml of DESS (20% DMSO and 0.25 M disodium EDTA, saturated with NaCl, pH 8.0)47 .
The meiofaunal size fraction was mechanically separated from the sand and concentrated by decanting five times with filtered tap water through a 45 μm filter. Subsequent separation from fine silt was achieved by repetitive centrifugation in 1.16 specific gravity LUDOX-TM solution48 . Following centrifugation, each sample was retained on a distinct mesh sieve, which was then folded, sliced and placed in a 15 ml falcon tube and kept at −80 °C until DNA extraction. Samples were lysed overnight at 55 °C in lysis buffer (100 mM Tris–HCl, pH7.5; 100 mM NaCl; 100 mM EDTA; 1% SDS, 500 μg ml−1 proteinase K), assisted by spinning wheel mixing, and DNA extracted with the QIAamp DNA Blood Maxi Kit (Qiagen) following the manufacturer's protocol.
Fonseca V.G., Carvalho G.R., Sung W., Johnson H.F., Power D.M., Neill S.P., Packer M., Blaxter M.L., Lambshead P.J., Thomas W.K, & Creer S. (2010). Second-generation environmental sequencing unmasks marine metazoan biodiversity. Nature Communications, 1(7), 98-.
Publication 2010
Biopharmaceuticals BLOOD Buffers Centrifugation Desmosine Edetic Acid EDTA, Disodium Endopeptidase K Marijuana Abuse Marines Sequence Analysis Sodium Chloride Sulfoxide, Dimethyl Tromethamine
2
RNA Oligonucleotide Synthesis and Purification
DNA oligonucleotides were purchased with standard desalting purification (Thermo Fisher Scientific). Short RNA oligonucleotides under 20-nt and construct 12 were synthesized on a MerMade synthesizer (BioAotomation) using standard phosphoramidite chemistry and deprotected as previously described (16 (link)).
Long RNA oligonucleotides were transcribed in vitro from synthetic ssDNA templates (Thermo Fisher Scientific). Equimolar amounts of ssDNA template and a universal T7 promoter sequence were annealed. One microgram annealed template was then used in a 100 μl transcription reaction containing 5 mM each rNTP, 22 mM magnesium chloride, 40 mM Tris–HCl pH 8.0, 2 mM spermidine, 10 mM DTT, 0.01% Triton X-100, 40 units RNase inhibitor (New England Biolabs) and 5 μl T7 RNA polymerase. Transcription reactions were incubated at 37°C for 2 h.
All RNA was gel purified by denaturing gel electrophoresis. Appropriate bands were excised and RNA was extracted by crush soak in 10 mM MOPS pH 6.0, 300 mM sodium chloride, and 1 mM disodium-EDTA. After extraction, RNA was ethanol precipitated, washed once with 70% ethanol and resuspended in water.
Dickey T.H, & Pyle A.M. (2017). The SMAD3 transcription factor binds complex RNA structures with high affinity. Nucleic Acids Research, 45(20), 11980-11988.
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Publication 2017
bacteriophage T7 RNA polymerase DNA, Single-Stranded EDTA, Disodium Electrophoresis Endoribonucleases Ethanol Magnesium Chloride morpholinopropane sulfonic acid Oligonucleotides phosphoramidite Sodium Chloride Spermidine Transcription, Genetic Triton X-100 Tromethamine
3
Hyperpolarized Lactate and Pyruvate Preparation
Dynamic nuclear polarization of the substrates was performed using a HyperSense system (Oxford Instruments Molecular Biotools, Oxford, UK) operating at 1.4 K and using a microwave power of 25 mW. For the lactate sample, 75 mg of 1.7-M sodium lactate in 37.5:62.5 w/w water:glycerol with 15-mM OX063 were mixed with approximately 7 μL of a 1:50-solution of Dotarem. Dissolution with 4 g of 40 mM Tris pH 7.6 containing 100-mg/L disodium EDTA yielded a final polarized solution of 33-mM lactate. Sample preparation and solvent for the 80-mM pyruvate solution was the same as previously described (23 (link)). The build-up time constant for the solid state polarization of lactate was on the order of 2800 s, almost three times a long as for pyruvate (1100 s). Whereas the polarization of the pyruvate samples had very little variation, 23.5% ± 0.5% (mean ± standard deviation (sd)), n = 6), the polarization of the lactate samples ranged from 15% to 31% with an average value of 25.3% ± 5.5% (n=12). The liquid-state polarization was estimated from the solid-state polarization build-up curve (scale in arbitrary units) for each sample and independent calibration experiments in which the liquid-state polarization of a sample was measured in the MR scanner. A pulse-and-acquire sequence (5.625° hard pulse, spectral width (SW) = 5000 Hz, TR = 3 s, N = 80) was used to measure the longitudinal relaxation constant of the hyperpolarized sample, which was then used to extrapolate the signal intensity to the time of dissolution. After doping the sample with a relaxation agent (10 μL/mL, Magnevist, Bayer Healthcare Pharmaceuticals, Wayne, NJ), a second acquisition (90° excitation, TR = 10 s, N = 100) was performed to estimate the signal intensity at thermal equilibrium. This estimation assumed a single T1 for the longitudinal decay and that the loss of polarization that occurred during the dissolution process did not change appreciably since performing the calibration measurements.
Mayer D., Yen Y.F., Josan S., Park J.M., Pfefferbaum A., Hurd R.E, & Spielman D.M. (2012). Application of Hyperpolarized [1-13C]Lactate for the In Vivo Investigation of Cardiac Metabolism. NMR in biomedicine, 25(10), 1119-1124.
Publication 2012
Dotarem EDTA, Disodium Glycerin Lactate, Sodium Lactates Magnevist Microwaves Pharmaceutical Preparations Pulse Rate Pyruvates Solvents Tromethamine
4
EDTA Chelation and Multivitamin Trial for CVD

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Publication 2011
Angina Pectoris bis(tetraheptylammonium)tetraiodocyclopentane tellurate(IV) Cerebrovascular Accident Dietary Supplements EDTA, Disodium Heart Hospitalization Minerals Myocardial Infarction Pharmaceutical Preparations Physicians Placebos Therapeutics Vitamins
5
Quantifying Protein Corona Formation on Nanoparticles
Plasma and serum samples (BioIVT, Hicksville NY) were diluted 1:5 in a dilution buffer composed of TE buffer (10 mM Tris, 1 mM disodium EDTA, 150 mM KCl) with 0.05% CHAPS. NP powder was reconstituted by sonicating for 10 min in DI water followed by vortexing for 2–3 sec. To form the protein corona, 100 µL of NP suspension (SP-003, 5 mg/ml; SP-007, 2.5 mg/ml; SP-011, 10 mg/ml) was mixed with 100 µL of diluted biological samples in microtiter plates. The plates were sealed and incubated at 37 °C for 1 h with shaking at 300 rpm. After incubation, the plate was placed on top of a magnetic collection device for 5 min to draw down the NPs. Unbound proteins in supernatant were pipetted out. The protein corona was further washed with 200 µL of dilution buffer three times with magnetic separation.
For the 10-NP screen, the five additional assay conditions evaluated were identical to those described above, with one of the following exceptions. First, a low concentration of NPs was evaluated that was 50% the original concentration (ranging from 2.5–15 mg/ml for each NP, depending on expected peptide yield). For the second and third assay variations, both low and high NP concentrations were run using an undiluted, neat plasma rather than diluting the plasma in buffer. For the fourth and fifth assay variations, both low and high NP concentrations were run using a pH 5 citrate buffer for both dilution and rinse.
To digest the proteins bound onto NPs, a trypsin digestion kit (iST 96×, PreOmics, Germany) was used according to protocols provided. Briefly, 50 µL of Lyse buffer was added to each well and heated at 95 °C for 10 min with agitation. After cooling the plates to room temperature, trypsin digestion buffer was added, and the plate incubated at 37 °C for 3 h with shaking. The digestion process was stopped with a stop buffer. The supernatant was separated from the NPs by a magnetic collector and further cleaned up by a peptide cleanup cartridge included in the kit. The peptide was eluted with 75 µL of elution buffer twice and combined. Peptide concentration was measured by a quantitative colorimetric peptide assay kit from Thermo Fisher Scientific (Waltham, MA).
Blume J.E., Manning W.C., Troiano G., Hornburg D., Figa M., Hesterberg L., Platt T.L., Zhao X., Cuaresma R.A., Everley P.A., Ko M., Liou H., Mahoney M., Ferdosi S., Elgierari E.M., Stolarczyk C., Tangeysh B., Xia H., Benz R., Siddiqui A., Carr S.A., Ma P., Langer R., Farias V, & Farokhzad O.C. (2020). Rapid, deep and precise profiling of the plasma proteome with multi-nanoparticle protein corona. Nature Communications, 11, 3662.
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Publication 2020
3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate Biological Assay Biopharmaceuticals Buffers Citrates Colorimetry Digestion Digestive System Processes EDTA, Disodium Peptides Plasma Powder Protein Corona Proteins Serum Technique, Dilution Tromethamine Trypsin

Most recents protocols related to «EDTA, Disodium»

1
5% Benzoyl Peroxide Topical Composition
Not available on PMC !

Example 7

A composition comprising 5% Benzoyl peroxide (BPO) as active ingredient:

IngredientsConcentration (w/w %)
Benzoyl peroxide (BPO)5.00
Ethoxydiglycol9.90
Glycerin8.00
Silica microspheres2.50
Carbomer0.60
Imidazolidinyl Urea0.30
PEG-40 Hydrogenated0.20
Disodium EDTA0.10
Sodium Hydroxide0.16
Waterq.s. 100%

The process for the preparation of the compositions listed above was as follows:

    • 1. Disodium EDTA and Carbomer were added to the water and homogenized;
    • 2. Glycerin was added to stage 1 and the mixture was stirred;
    • 3. PED-40 hydrogenated castor oil was heated to 40° C. separately and after clear liquid was obtained, it was added to stage 2;
    • 4. 20% solution of sodium hydroxide was added for neutralization;
    • 5. A solution of imidazolidinyl urea in water was added to stage 4;
    • 6. Benzoyl peroxide was added to ethoxydiglycol separately and passed through Fryma colloid mill, twice;
    • 7. Silica microspheres were added to the stage 6 and resultant mixture was stirred;
    • 8. Stage 7 was added to stage 5 and the mixture was homogenized.

US11865208B2. Pharmaceutical compositions comprising silica microspheres (2024-01-09). SOL-GEL TECHNOLOGIES LTD. [IL]. Inventors: Marina Shevachman [IL], Amira Ze' Evi [IL], Eilon Asculai [IL], Batella Benyaminovich [IL], Nir Avram [IL], Chaim Aschkenasy [IL].
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Patent 2024
carbomer Castor oil Colloids EDTA, Disodium Glycerin hydroxide ion imidazolidinyl urea Microspheres Peroxide, Benzoyl Pharmaceutical Preparations Silicon Dioxide Sodium-20 Sodium Hydroxide urea-EDTA
2
Hydrocortisone Formulations: Concentration Equivalency
Not available on PMC !

Example 4

Equivalent to:Equivalent to:Equivalent to:Equivalent to:
Formulation:50 mg/ml100 mg/ml100 mg/ml100 mg/ml
Componenthydrocortisonehydrocortisonehydrocortisonehydrocortisone
Hydrocortisone67.1 mg6.71%134.2 mg13.42%6710.0 mg13.42%2,013.0 g13.42% 
sodium phosphate
Monobasic sodium1.0 mg0.10%1.0 mg0.10%50.0 mg0.10%19.5 g(1) 0.13%(1)
phosphate
Dibasic sodium10.9 mg1.09%10.9 mg1.09%545.0 mg1.09%205.5 g (2) 1.37% (2)
phosphate
Disodium EDTA0.2 mg0.02%0.2 mg0.02%10.0 mg0.02%3.0 g0.02%
Monothioglycerol5.0 mg0.50%5.0 mg0.50%250.0 mg0.50%75.0 g0.50%
Sodium hydroxide
Water (Q. S.)1 mL1 mL50 mL15,900.0 g (3)
(1)Monobasic sodium phosphate dihydrate
(2) Dibasic sodium phosphate dihydrate
(3) Equivalent to 15 Liter

US11857555B2. Aqueous pharmaceutical formulation of hydrocortisone sodium phosphate and monothioglycerol (2024-01-02). ANTARES PHARMA, INC. [US]. Inventors: Xiaoming Chen [US], Shaowei Ong [US].
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Patent 2024
EDTA, Disodium hydrocortisone sodium phosphate hydroxide ion Phosphates Sodium Hydroxide sodium phosphate sodium phosphate, dibasic sodium phosphate, monobasic thioglycerol
3
Sperm DNA Integrity Analysis by Flow Cytometry
The sperm aliquots stained with AO were also analyzed using the flow cytometry technique. Briefly, 0.2 mL of thawed samples with 1 million events was diluted with TNE buffer (0.01 M Tris–HCl, 0.15 M NaCl, and 1 mM EDTA, pH: 7.4)
containing 10% glycerol at a density of 1-2×106 sperm/mL. Then, they were immediately mixed with 0.4 mL of the solution containing 0.1% Triton X-100, 0.15 M NaCl,
and 0.08 N HCl (pH 1.2) at 4 °C. After 30 seconds, they were incubated in 1.2 mL of AO at a 6 μg/mL concentration in a solution containing 0.037 M citric acid, 0.126 M Na2HPO4,
0.001 M disodium EDTA, and 0.15 M NaCl (pH 6.0) at 4 °C. These were analyzed using the FACSCaliburTM flow cytometer. Strong green (FL-1)
and negative red fluorescence (FL-2) depicted normal sperm integrity (excitation wavelength at 488 nm and emission wavelengths at 520 nm for FL1 and 640 nm for FL3).
A sample of acid-treated sperm was used as a positive control. DNAf index (DFI) was calculated using the formula below and expressed as a percentage.
DFI=Mean value of red fluorescence/(mean value of red+green fluorescence).
AO shows green fluorescence in the monomeric state (when it binds to DNA) and red fluorescence in the polymeric state (when it binds to RNA or denatured single-stranded DNA). Larger cells with higher histone and lower protamine content are found in the upper quartile of the dot blot chart. These cell populations show high DNA stainability (HDS) and represent immature cells. 17 (link)
Kermani T., Hosseini S.F., Talaei-Khozani T, & Aliabadi E. (2023). Effect of Pre-Incubation of Cryopreserved Sperm with either Kisspeptin or Glutathione to Mitigate Freeze-Thaw Damage. Iranian Journal of Medical Sciences, 48(2), 198-208.
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Publication 2023
Buffers Cells Citric Acid DNA, Single-Stranded Dot Immunoblotting Edetic Acid EDTA, Disodium Flow Cytometry Fluorescence Glycerin Histones Neoplasm Metastasis Polymers Population Group Protamines Sodium Chloride Sperm spermic acid Triton X-100 Tromethamine
4
Superoxide Dismutase Activity Assay
Cladode chlorenchyma tissues (0.5 g) were collected at 12:00, frozen in liquid nitrogen, and immediately ground with a mortar and pestle. They were ground again with 20 mg of Polyclar AT in 2 ml of grinding medium, which contained 50 mM Tris·HCl (pH 7.5), 5 mM dithiothreitol, 0.2 mM disodium EDTA, 0.5% (w/v) polyvinylpyrrolidone, and 1% (v/v) Triton X-100. The homogenate was centrifuged at 10 000 × g for 10 min at 4 °C, and the supernatant was concentrated about tenfold in Amicon Ultra-2 centrifugal filter devices (Merck Millipore Ltd., MA, USA). We used a SOD Assay Kit-WST (Dojindo Co., Kumamoto, Japan), in which the superoxide anion reduces WST-1 ((2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2 4-disulphophenyl)-2H-tetrazolium, monosodium salt) to yellow formazan, which can be quantified from absorbance at 450 nm. Antioxidants inhibit yellow WST-1 formation. In brief, the supernatants were mixed with WST-1 and then with the enzyme solution and incubated at 37 °C for 20 min. Absorbance at 450 nm was measured in a spectrophotometer. One unit of enzyme activity was defined as the amount of enzyme that caused a 50% decrease in formazan formation. The activity was expressed as units per milligram of protein, and the relative value (%) was based on the value in the control at the start of treatment. Protein contents were determined with the Bradford reagent (Sigma Aldrich Co., St. Louis, MO, USA) according to Bradford (1976 (link)). The activity was calculated as the mean of 3 measurements (three plants per treatment).
Kondo A., Ito M., Takeda Y., Kurahashi Y., Toh S, & Funaguma T. (2023). Morphological and antioxidant responses of Nopalea cochenillifera cv. Maya (edible Opuntia sp. “Kasugai Saboten”) to chilling acclimatization. Journal of Plant Research, 136(2), 211-225.
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Publication 2023
4-nitrophenyl Antioxidants Biological Assay Cardiac Arrest Dithiothreitol EDTA, Disodium enzyme activity Enzymes Formazans Freezing Medical Devices Nitrogen Plants Povidone Proteins Sodium Chloride Superoxides Tetrazolium Salts Tissues Triton X-100 Tromethamine yellow-1
5
Stabilizing Excipients for PF-06439535 Formulation
An important aspect of the formulation development program was to determine the stabilizing excipients for PF-06439535. sucrose (85 mg/mL), edetate disodium dihydrate (EDTA; 0.05 mg/mL), and polysorbate 80 (PS80; 0.2 mg/mL) were evaluated for use in the PF-06439535 formulation. Analytical grade sucrose (EMD Millipore Corporation, Burlington, MA, USA), EDTA (JT Baker, Avantor, Radnor, PA, USA), and PS80 (JT Baker, Avantor, Radnor, PA, USA) were used. To determine if sucrose was an appropriate cryoprotectant for frozen storage of PF-06439535 drug substance, a formulation at the drug substance protein concentration of 100 mg/mL with all excipients (20 mM succinate at pH 5.5, 85 mg/mL sucrose, 0.05 mg/mL EDTA, 0.2 mg/mL PS80) was evaluated after 22 weeks at − 20 °C and − 40 °C. Additionally, PF-06439535 drug product at 25 mg/mL with all excipients (20 mM succinate at pH 5.5, 85 mg/mL sucrose, 0.05 mg/mL EDTA, 0.2 mg/mL PS80) was evaluated after 22 weeks at 5 °C and 25 °C and after 8 weeks at 40 °C. Finally, PF-06439535 in the succinate formulation (five lots) was compared with PF-06439535 in the RP formulation (one lot) and to bevacizumab-US and bevacizumab-EU (three lots each) after storage at 40 °C for 12 weeks.
Ingram R.L, & Weiser S.E. (2023). Development of the Drug Product Formulation of the Bevacizumab Biosimilar PF-06439535 (Bevacizumab-bvzr). Drugs in R&D, 23(1), 55-64.
Publication 2023
Bevacizumab Cryoprotective Agents Drug Storage Edetic Acid EDTA, Disodium Excipients Freezing Pharmaceutical Preparations Polysorbate 80 Program Development Staphylococcal Protein A Succinate Sucrose

Top products related to «EDTA, Disodium»

1
Disodium edta by Merck Group
Sourced in United States, United Kingdom, Germany
Disodium EDTA is a chemical compound commonly used as a laboratory reagent. It functions as a chelating agent, capable of binding to and forming stable complexes with metal ions. This property makes it useful in various analytical and purification applications.
2
Sodium chloride (nacl) by Merck Group
Sourced in United States, Germany, United Kingdom, India, Italy, France, Spain, China, Canada, Sao Tome and Principe, Poland, Belgium, Australia, Switzerland, Macao, Denmark, Ireland, Brazil, Japan, Hungary, Sweden, Netherlands, Czechia, Portugal, Israel, Singapore, Norway, Cameroon, Malaysia, Greece, Austria, Chile, Indonesia
NaCl is a chemical compound commonly known as sodium chloride. It is a white, crystalline solid that is widely used in various industries, including pharmaceutical and laboratory settings. NaCl's core function is to serve as a basic, inorganic salt that can be used for a variety of applications in the lab environment.
3
Triton x 100 by Merck Group
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Triton X-100 is a non-ionic surfactant commonly used in various laboratory applications. It functions as a detergent and solubilizing agent, facilitating the solubilization and extraction of proteins and other biomolecules from biological samples.
4
Methanol by Merck Group
Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan
Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
5
Acetonitrile by Merck Group
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Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.
6
Gallic acid by Merck Group
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Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.
7
Formic acid by Merck Group
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Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.
8
Perchloric acid by Merck Group
Sourced in United States, Germany, United Kingdom, Poland, Italy, Sao Tome and Principe, Brazil, Spain, India, China, Malaysia, Macao, Belgium, Switzerland
Perchloric acid is a strong oxidizing agent commonly used in analytical chemistry. It is a colorless, fuming liquid with a pungent odor. Perchloric acid is used in various laboratory applications, including sample digestion, oxidation reactions, and the preparation of perchlorate salts.
9
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
10
Tris hcl by Merck Group
Sourced in United States, Germany, India, Italy, United Kingdom, Sao Tome and Principe, France, Hungary, Spain, China, Canada, Macao, Sweden, Australia, Poland, Switzerland
Tris-HCl is a buffer solution used in various laboratory applications. It is a mixture of tris(hydroxymethyl)aminomethane (Tris) and hydrochloric acid (HCl). This buffer solution is widely employed to maintain a specific pH range in experiments, particularly in the field of biochemistry and molecular biology.

More about "EDTA, Disodium"

Ethylenediaminetetraacetic acid, disodium salt (Disodium EDTA) is a versatile chelating agent widely used in biochemical and biomedical research.
As a divalent cation sequestrant, it functions by binding to metal ions like calcium (Ca2+) and magnesium (Mg2+), which are essential for various cellular processes.
This property makes Disodium EDTA a valuable tool for controlling the availability of these ions in cell culture media, blood collection tubes, and other applications where the regulation of divalent cations is crucial.
Researchers rely on Disodium EDTA to maintain the integrity and stability of biomolecules, cells, and tissues during experimentation, helping to enhance the reproducibility of their findings.
The compound is often used in conjunction with other reagents like sodium chloride (NaCl), Triton X-100, methanol, acetonitrile, gallic acid, formic acid, and perchloric acid, which may be employed for sample preparation, protein extraction, or analysis purposes.
Disodium EDTA's chelating abilities also make it useful for preserving the quality of cell culture media components, such as fetal bovine serum (FBS), and for stabilizing biomolecules like proteins and nucleic acids by sequestering divalent cations that can lead to degradation.
Additionally, Tris-HCl buffers are frequently used alongside Disodium EDTA to maintain optimal pH and ionic conditions for various biochemical assays and procedures.
By understanding the versatile nature and applications of Disodium EDTA, researchers can optimize their experimental protocols, enhance the reproducibility of their findings, and unlock new insights in the fields of biochemistry, cell biology, and biomedical research.