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Effectene

Effectene is a transfection reagent used to introduce nucleic acids, such as plasmids or siRNA, into mammalian cells.
It employs a unique lipid-based formulation that forms compact complexes with DNA, enhancing cellular uptake and minimizing cytotoxicity.
Effectene enables efficient gene expression or gene silencing studies, streamlining research into cellular processes and disease mechanisms.
Its ease of use and high transfection efficiencies make it a versatile tool for a range of molecular and cell biology applications.

Most cited protocols related to «Effectene»

1
Mammalian Cell Expression of Fluorescent Proteins
Cited 27 times
The plasmids used for expression of all FPs in mammalian cells were based on pEGFP-N1 backbone (Clontech). To construct the plasmids (piRFP670-N1, piRFP682-N1, piRFP702-N1, piRFP713-N1, piRFP720-N1, pmNeptune-N1, pE2-Crimson-N1, peqFP650-N1, peqFP670-N1) the respective genes were PCR amplified as AgeI-NotI fragments and swapped with a gene of EGFP in pEGFP-N1.
To construct pMito-iRFP670, pMito-iRFP713, and pMito-iRFP720 iRFP670, iRFP713, and iRFP720 genes were cut from N1 plasmids and swapped with mTagBFP gene in pMito-mTagBFP22 (link). To generate pNLS-iRFP670, pNLS-iRFP713, and pNLS-iRFP720 iRFP670, iRFP713, and iRFP720 genes were cut from piRFPs-N1 plasmids AgeI-NotI fragments and swapped with a gene of ECFP in the pNLS-ECFP plasmid.
HeLa cell lines were grown in DMEM containing 10% FBS, 0.5% penicillin-streptomycin and 2 mM glutamine (Invitrogen). For microscopy cells were cultured in 35 mm glass bottom culture dishes with no. 1 cover glasses (MatTek). MTLn3 rat adenocarcinoma cells were cultured in αMEM medium (Life Technologies) supplied with 5% FBS, 0.5% penicillin-streptomycin, and 2 mM glutamine (Life Technologies). Plasmid transfections were performed using an Effectene reagent (Qiagen) according to the manufacturer’s protocol. Stably-expressing cells were selected with 700 mg/ml G418 antibiotic. Sorting of positive cells was performed using a MoFlo XDP sorter (Beckman Coulter) equipped with a 676 nm Kr laser and a 700 nm LP emission filter.
Shcherbakova D.M, & Verkhusha V.V. (2013). Near-infrared fluorescent proteins for multicolor in vivo imaging. Nature methods, 10(8), 751-754.
Publication 2013
Adenocarcinoma antibiotic G 418 Antibiotics Cell Lines Cells Culture Media Effectene Eyeglasses Genes Glutamine HeLa Cells Hyperostosis, Diffuse Idiopathic Skeletal Mammals Microscopy Penicillins Plasmids Streptomycin Transfection Vertebral Column
2
Biochemical Analysis of Wnt and Hedgehog Signaling
Cited 24 times
Biochemical studies involving L-Wnt-STF or DLD-1 cells were performed in either 48- or 6-well format with IWR (10μM) or IWP (5μM) compounds and/or cycloheximide (100μM) in a 48 hr assay period. E-cadherin depletion studies were performed at 4°C using DLD-1 cells lysed in PBS/1% NP-40/protease inhibitors. For Wnt3A and ShhN phase separation assays, expression constructs encoding mPorcn, hWnt3A-myc, or mShhN were transfected into HEK293 cells using Effectene (Qiagen). After 48 hrs, cells were lysed for 15 min, RT with distilled water, 10 mM Tris-HCl, 150 mM NaCl /1% TritonX-114 (PL buffer). Lysate was briefly chilled on ice, pelleted for 10 min 4°C, and the supernatant combined with an equal volume of PL buffer/3.5% TX-114. Solutions were rotated for 15 min at 4°C, placed at 37°C for 5 min followed by an additional centrifugation for 5 min at 2000g, RT. Distinct phases were collected and combined with PL buffer to a total volume of 1 ml. Samples were chilled on ice, ConA sepharose (for Wnt protein) or 5E1 mAb/protein A sepharose (for ShhN protein) added, and samples rotated for 2 hrs at 4°C. Beads were washed 2x with PL buffer, and a Western blot performed with eluted proteins using an anti-c-myc antibody. For IWP-PB and IWR-PB binding studies, cell lysate (10mM Na2HPO4 pH7.4, 0.15mM NaCl, 1%NP-40) derived from HEK293 cells transfected with the Porcn-myc or various Axin2 constructs, or bacterially expressed Axin2 (508-761)-GST protein were incubated with either DMSO, linker (0.17 mM), IWP-3 (0.6 mM), or IWR-1 (0.2mM) for 1 hr at 4°C prior to addition of NeutrAvidin agarose resin (Pierce) and either DMSO, IWP-PB (0.17 mM) or IWR-PB (.05mM). Samples were rotated overnight at 4°C, washed with 3×10min with lysis buffer, and bound material eluted with sample loading buffer. For in vitro trypsinization studies, lysate derived from HEK293 cells expressing Axin2-myc protein and treated with or without IWR-1 was incubated at RT with 0.25 mg/ml of trypsin and 0.10 mM EDTA for indicated time periods.
Chen B., Dodge M.E., Tang W., Lu J., Ma Z., Fan C.W., Wei S., Hao W., Kilgore J., Williams N.S., Roth M.G., Amatruda J.F., Chen C, & Lum L. (2009). Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature chemical biology, 5(2), 100-107.
Publication 2008
anti-c antibody AXIN2 protein, human Biological Assay Buffers Cells Centrifugation concanavalin A-sepharose Cycloheximide E-Cadherin Edetic Acid Effectene HEK293 Cells neutravidin Nonidet P-40 Proteins Resins, Plant Sepharose SERPINA1 protein, human Sodium Chloride Staphylococcal protein A-sepharose Sulfoxide, Dimethyl Tromethamine Trypsin Western Blot Wnt Proteins
3
Cellular Transfection and Gene Silencing Protocol
Cited 18 times
HeLa, COS-1, and COS-7 cells were grown as described previously (Akhmanova et al., 2001 (link)). Effectene or Superfect transfection reagents (QIAGEN) were used for plasmid transfection. Mass transfection of COS-1 cells was performed by DEAE-dextran method (Akhmanova et al., 2001 (link)). Stable clones were selected in the presence of 0.3–0.4 mg/ml G418 (Calbiochem). Synthetic siRNAs (Proligo) were transfected, using Oligofectamine (Invitrogen). SiRNAs were directed against the following target sites: CLASP1#A, GCCATTATGCCAACTATCT; CLASP1#B, GGATGATTTACAAGACTGG; CLASP2#A, GTTCAGAAAGCCCTTGATG; CLASP2#B, GACATACATGGGTCTTAGA; control (scrambled CLASP1#A), GCACTCATTATGACTCCAT. 7–10% confluent HeLa cells were transfected, using Oligofectamine (Invitrogen) with siRNAs at the minimal effective concentration (10 nM for CLASP1#A and CLASP1#B, 200 nM for CLASP2#A and CLASP2#B), whereas the control siRNA was used at 200 nM concentration.
Mimori-Kiyosue Y., Grigoriev I., Lansbergen G., Sasaki H., Matsui C., Severin F., Galjart N., Grosveld F., Vorobjev I., Tsukita S, & Akhmanova A. (2005). CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex. The Journal of Cell Biology, 168(1), 141-153.
Publication 2005
antibiotic G 418 Clone Cells COS-1 Cells COS-7 Cells COS Cells DEAE-Dextran Effectene HeLa Cells oligofectamine Plasmids RNA, Small Interfering Transfection
4
Generation and Characterization of mTOR Knockout Mice
Cited 17 times
The generation of the mTORneo embryonic stem (ES) clones used to functionally inactivate the mTOR gene was described previously (Gangloff et al., 2004 (link)). In brief, a targeting vector was produced by introducing a loxP site upstream of the mTOR promoter region and a neo cassette flanked by two loxP sites in the intron preceding exon 6. To generate mTORneo ES cells, the construct was electroporated into 129SVJae ES cells, and G418-resistant clones were screened for homologous recombination by Southern blotting. For excision of the floxed PGKneo cassette, the mTORneo ES cells were transfected with 0.8 µg pEGFP-N1 (Takara Bio Inc.) and 0.2 µg pMC-Cre using transfection reagent (Effectene; QIAGEN). ES cells expressing EGFP were cultured in the presence and absence of G418. G418-sensitive colonies were analyzed by PCR. ES cells showing the selective deletion of the neomycin resistance cassette and containing the mTOR floxed allele were injected into C57BL/6J blastocysts to generate chimeric mice. Chimeric mice were mated with C57BL/6J mice to generate the heterozygous floxed mutant (mTOR+/flox) through germline transmission. Positive F1 offspring were identified by PCR and further backcrossed with C57BL/6J mice during five generations (F5 offspring). mTOR+/flox mice were interbred to obtain a homozygous mutant (mTORflox/flox). mTORflox/flox (control) mice were bred with HSA-Cre mice to generate Cre-positive mTORflox/flox (mTOR−) mice. ES cells and mice were genotyped by PCR.
The primers used to identify the mTOR flox allele were p2 (5′-GCTCTTGAGGCAAATGCCACTATCACC-3′) and p3 (5′-TCATTACCTTCTCATCAGCCAGCAGTT-3′), and the primers to identify the recombined mTOR− allele were p1 (5′-TTCATTCCCTTGAAAGCCAGTCTCACC-3′) and p3. The animals were provided with mouse chow and water ad libitum in a restricted-access, specific pathogen–free animal care facility at the Ecole Normale Superieure of Lyon (Plateau de Biologie Experimentale de la Souris). All procedures were performed in accordance with national and European legislation on animal experimentation.
Risson V., Mazelin L., Roceri M., Sanchez H., Moncollin V., Corneloup C., Richard-Bulteau H., Vignaud A., Baas D., Defour A., Freyssenet D., Tanti J.F., Le-Marchand-Brustel Y., Ferrier B., Conjard-Duplany A., Romanino K., Bauché S., Hantaï D., Mueller M., Kozma S.C., Thomas G., Rüegg M.A., Ferry A., Pende M., Bigard X., Koulmann N., Schaeffer L, & Gangloff Y.G. (2009). Muscle inactivation of mTOR causes metabolic and dystrophin defects leading to severe myopathy. The Journal of Cell Biology, 187(6), 859-874.
Publication 2009
Alleles Animals antibiotic G 418 Blastocyst Chimera Clone Cells Cloning Vectors Deletion Mutation Effectene Embryo Embryonic Stem Cells Europeans Exons FRAP1 protein, human Genes Germ Line Heterozygote Homologous Recombination Homozygote Introns Mice, Inbred C57BL Mus Neomycin Oligonucleotide Primers Specific Pathogen Free Stem, Plant Transfection Transmission, Communicable Disease
5
Comprehensive Cell Culture and Antibody Protocol
Cited 14 times
HEK293T, HeLa and PINK1 KO35 cells were cultured in Dulbecco's modified eagle medium (Life Technologies) supplemented with 10% (v/v) Fetal Bovine Serum (Gemini Bio Products), 10 mM HEPES (Life Technologies), 1 mM Sodium Pyruvate (Life Technologies), nonessential amino acids (Life Technologies) and GlutaMAX (Life Technologies). HeLa cells were acquired from the ATCC and authenticated by the Johns Hopkins GRCF Fragment Analysis Facility using STR profiling. All cells were tested for mycoplasma contamination bimonthly using the PlasmoTest kit (InvivoGen). Transfection reagents used were: Effectene (Qiagen), Lipofectamine LTX (Life Technologies), Avalanche-OMNI (EZ Bio-systems), X-tremeGENE HP (Roche) and X-tremeGENE 9 (Roche).
Rabbit monoclonal and polyclonal antibodies used: Beclin, pULK1-S317, pULK1-S757, TBK1, pTBK1-S172, NDP52, TAX1BP1, ATG5, Actin, and HA (Cell Signaling Technologies); GAPDH and LC3B (Sigma); ULK1 and Tom20 (Santa Cruz Biotechnology); Optineurin (OPTN) (Proteintech); GFP (Life Technologies); pSer65 ubiquitin (Millipore) and Mfn1 was generated previously36 (link). Mouse monoclonal antibodies used: NBR1 and p62 (Abnova), Cytochrome C oxidase subunit II (CoxII, Abcam), Parkin (Santa Cruz Biotechnology), DNA (Progen Biotechnik), ubiquitin (Cell Signaling). Chicken anti-GFP (Life Technologies) was also used. For catalog numbers see Supplementary Table 1. Human tissue panel blots were purchased (NOVUS Biologicals).
Lazarou M., Sliter D.A., Kane L.A., Sarraf S.A., Wang C., Burman J.L., Sideris D.P., Fogel A.I, & Youle R.J. (2015). The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Nature, 524(7565), 309-314.
Publication 2015
Actins Amino Acids Antibodies Avalanches Biological Factors Cells Chickens Culture Media cytochrome C oxidase subunit II Eagle Effectene Fetal Bovine Serum GAPDH protein, human HeLa Cells HEPES Homo sapiens Lipofectamine Monoclonal Antibodies Mus Mycoplasma Novus OPTN protein, human PARK2 protein, human Progens PTGS2 protein, human Pyruvate Rabbits Sodium TBK1 protein, human Tissues Transfection Ubiquitin ULK1 protein, human

Most recents protocols related to «Effectene»

1
3D Metastatic Bone Cancer Model

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Publication 2023
beta-tricalcium phosphate Cell Line, Tumor Cells Dietary Supplements Effectene Endothelial Cells Endothelium enhanced green fluorescent protein Green Fluorescent Proteins Homo sapiens Human Umbilical Vein Endothelial Cells Mesenchymal Stromal Cells Multipotent Mesenchymal Stromal Cells Neoplasms Neuroblastoma Osteocytes Penicillins Plasmids Reading Frames Regional Ethics Committees Streptomycin Transfection
2
Transfection and Luciferase Assay Protocol
For transfection experiments, MC were plated at 50% confluency and transfected with either 1 µg of the mouse TSP1 luciferase reporter construct [mTSP1-luciferase, a gift from P. Bornstein, Plasmid #12409, Addgene (Michaud-Levesque and Richard, 2009 (link))] with 0.05 µg pCMV β-galactosidase (β-Gal, Clonetech) using Effectene (Qiagen) or 100 nM of MTJ1 or control siRNA (Silencer Select, ThermoFisher) using Lipofectamine (Invitrogen). After 18 h, cells were serum-deprived and treated as above for protein collection for siRNA experiments. For luciferase harvest, 1× Reporter Lysis Buffer (Promega) was added to the plate which was then stored at −80°C overnight prior to cell lysis. Luciferase activity was measured on clarified lysate using the Luciferase Assay System (Promega) with a luminometer (Junior LB 9509, Berthold). Β-Gal activity was used to normalize transfection efficiency, measured using the β-Galactosidase Enzyme Assay System (Promega) with a SpectraMax Plus 384 Microplate Reader (Molecular Devices) set to read absorbance at 420 nm.
The pcDNA3.1 plasmid which contains GRP78 lacking its ER retention sequence KDEL was transfected by electroporation as previously described (Trink et al., 2022 (link)). This was used to overexpress GRP78 at the cell surface. The empty vector pcDNA 3.1 was used as a control.
Trink J., Ahmed U., O’Neil K., Li R., Gao B, & Krepinsky J.C. (2023). Cell surface GRP78 regulates TGFβ1-mediated profibrotic responses via TSP1 in diabetic kidney disease. Frontiers in Pharmacology, 14, 1098321.
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Publication 2023
beta-Galactosidase Biological Assay Buffers Cells Cloning Vectors Effectene Electroporation Enzyme Assays Glucose Regulated Protein 78 kDa Lipofectamine Luciferases Medical Devices Mus Plasmids Promega Proteins Retention (Psychology) RNA, Small Interfering Serum thrombospondin-1, human Transfection
3
Generating Murine Hh Protein Variants
We used murine Shh (Dierker et al., 2009 (link)) and Hh sequences (nucleotides 1–1416, corresponding to amino acids 1–471 of D. melanogaster Hh) that were generated from cDNA by PCR using primer sequences that can be provided upon request. PCR products were inserted into pENTR for sequence confirmation and subsequently into pUAST for protein expression in S2 cells or the generation of transgenic flies. Mutations were introduced via the QuickChange Lightning site-directed mutagenesis kit (Stratagene, La Jolla, United States). S2 cells were cultured in Schneider’s medium (Invitrogen, Carlsbad, United States) supplemented with 10% fetal calf serum and 100 μg/mL penicillin/streptomycin. The cells were transfected with constructs encoding Hh and Hh variants together with a vector encoding an actin-Gal4 driver using Effectene (Qiagen, Hilden, Germany) and cultured for 36 h in Schneider’s medium before protein was harvested from the supernatant.
Manikowski D., Steffes G., Froese J., Exner S., Ehring K., Gude F., Di Iorio D., Wegner S.V, & Grobe K. (2023). Drosophila hedgehog signaling range and robustness depend on direct and sustained heparan sulfate interactions. Frontiers in Molecular Biosciences, 10, 1130064.
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Publication 2023
Actins Amino Acids Animals, Transgenic Cells Cloning Vectors Diptera DNA, Complementary Effectene Fetal Bovine Serum Mus Mutagenesis, Site-Directed Mutation Nucleotides Oligonucleotide Primers Penicillins Proteins Protein S Streptomycin
4
Transcriptional Regulation in Pancreatic Cancer
The promoter reporter constructs for E2F2 (#MPRM38445-LvPG04-GC), E2F3 (MPRM40957-LvPG04-GC), CCNB1 (MPRM49947-LvPG04-GC) and CCNB2 (MPRM39222-LvPG04-GC) were purchased from BioCat GmbH (Heidelberg, Germany) and transduced into PKrasG12D/+and PKrasG12D/+;SnailKI/KI PDAC cell lines.
The Secrete-Pair Dual Luminescence Assay Kit (#LF032-GC, BioCat GmbH, Heidelberg, Germany) was used according to the manufacturer’s instructions to analyse reporter activity blinded to the genotype. In brief, cell lines transduced with the reporter constructs were seeded in 6-well plates and medium was collected after 24 h. For measurement of Gaussia Luciferase (GLuc), 10 µL of the collected culture medium were pipetted in duplicates into a white opaque 96-well plate. GLuc Assay Working Solution was prepared with Buffer GL-S according to the manufacturer’s instructions and 100 µL was added per well. After incubation for 1 min at room temperature, luminescence was measured using a CLARIOstar microplate reader (BMG Labtech GmbH). For transduction normalization, Secreted Alkaline Phosphatase (SEAP) was measured. Therefore, medium was heated at 65 °C for 15 min and SEAP Assay Working Solution prepared according to the manufacturer’s protocol. 100 µL SEAP Assay Working Solution were added to 10 µL medium per sample in duplicates in a white opaque 96-well plate. Luminescence was measured after 5 min incubation at room temperature in a CLARIOstar microplate reader (BMG Labtech GmbH). Ratios of the mean Gaussia Luciferase (GLuc) to the mean Secreted Alkaline Phosphatase (SEAP) were calculated to determine reporter activity.
pGL3 Basic luciferase reporter plasmids containing Cyclin D1 (Ccnd1) promoter fragments (RRID:Addgene_32727 and RRID:Addgene_32726)72 (link) were used to determine Cyclin D1 promoter activity blinded to the genotype. PKrasG12D/+and PKrasG12D/+;SnailKI/KI PDAC cell cultures were transfected with the reporter constructs using Effectene Transfection Reagent (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations. In each sample, 40 ng phRL-TK (Promega) Renilla Luciferase control reporter vector was co-transfected as an internal control for transfection efficiency. The medium was changed on the next day and the Dual-luciferase reporter assay system (Promega) was used according to the manufacturer’s instructions to determine luciferase activity 48 h post transfection.
Paul M.C., Schneeweis C., Falcomatà C., Shan C., Rossmeisl D., Koutsouli S., Klement C., Zukowska M., Widholz S.A., Jesinghaus M., Heuermann K.K., Engleitner T., Seidler B., Sleiman K., Steiger K., Tschurtschenthaler M., Walter B., Weidemann S.A., Pietsch R., Schnieke A., Schmid R.M., Robles M.S., Andrieux G., Boerries M., Rad R., Schneider G, & Saur D. (2023). Non-canonical functions of SNAIL drive context-specific cancer progression. Nature Communications, 14, 1201.
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Publication 2023
Alkaline Phosphatase Anophthalmia with pulmonary hypoplasia Biological Assay Buffers CCNB1 protein, human CCNB2 protein, human Cell Culture Techniques Cell Lines Cyclin D1 Effectene Genotype Luciferases Luciferases, Renilla Luminescence Luminescent Measurements Paragangliomas 3 Plasmids Promega Transfection
5
Characterization of P-body Interactions
HEp-2 (catalogue #CCL-23) and HEK293 (CRL-1573) cells were obtained from American Type Cell Collection (Manassas, VA) and maintained in DMEM supplemented with 10% fetal calf serum, L-glutamine (2 mM), penicillin (200 U/ml), and streptomycin (200 ug/ml). Cells were transfected using the Effectene transfection system (Qiagen, Valencia, CA) as directed by the manufacturer. HEp-2 cells were used for immunohistochemistry; twenty-four hours after transfection, cells were fixed with 2% paraformaldehyde in PBS and permeabilized with 0.2% triton X-100 in PBS. Cells were stained with primary and secondary antisera as indicated.
To investigate the interaction between NLS-EDC4 and PATL1, cells were transfected with both plasmids and subsequently treated for 2 hours with leptomycin B (LMB; 10nM) to inhibit nuclear export.
To deplete endogenous P-bodies from HEp-2 cells, cells were transfected with siRNA directed against LSm14a, DDX6, or with scrambled siRNA using Dharmafect (Dharmacon, Lafayette, CO) as directed by the manufacturer. Twenty-four hours after treatment with siRNA, cells were transfected with plasmids encoding P-body components. Cells were subsequently fixed, permeabilized and stained with primary and secondary antisera.
To permit semi-quantitative analysis of the interaction between P-body components in the two-hybrid assay, the number of transfected cells expressing two (or more) P-body components in nuclear dots was compared to the total number of cells expressing two (or more) of the transfected proteins. Three separate transfections were performed and at least 30 cells expressing two (or more) proteins were scored in each transfection. Results are presented as mean +/- SEM.
For co-immunoprecipitation assays, HEK293 cells were transfected with plasmids encoding GFP-EDC4 and monomeric cherry (mCh) fused to XRN1(1232–1706), GFP-EDC4(630–1437 and mCh-XRN1(1232–1706) or with mCh-XRN1(1232–1706) alone. Twenty-four hours after transfection, cells were incubated with lysis buffer containing Tris-HCl pH7.4 (25 mM), NaCl (150 mM), EDTA (1mM), NP-40 (1%) and glycerol (5%). The GFP-containing fusion proteins were immunoprecipitated using GFP-Trap magnetic agarose, as directed by the manufacturer (Proteintech, Rosemont, Illinois). Proteins were eluted from the magnetic agarose by boiling in 2X sample buffer.
Bloch D.B., Sinow C.O., Sauer A.J, & Corman B.H. (2023). Assembly and regulation of the mammalian mRNA processing body. PLOS ONE, 18(3), e0282496.
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Publication 2023
Aftercare Biological Assay Buffers Cardiac Arrest Cell Body Cells Co-Immunoprecipitation DDX6 protein, human Edetic Acid Effectene Fetal Bovine Serum Glutamine Glycerin HEK293 Cells Human Body Immune Sera Immunohistochemistry leptomycin B Nonidet P-40 Nuclear Export paraform Penicillins Plasmids Processing Bodies Proteins Prunus cerasus RNA, Small Interfering Sepharose Sodium Chloride Streptomycin Transfection Triton X-100 Tromethamine Two-Hybrid System Techniques XRN1 protein, human

Top products related to «Effectene»

1
Effectene transfection reagent by Qiagen
Sourced in Germany, United States, Netherlands, Spain, Japan, China, United Kingdom, Singapore
Effectene Transfection Reagent is a lipid-based transfection reagent used for the efficient delivery of DNA, RNA, proteins, and other macromolecules into a variety of eukaryotic cell types. It is designed to facilitate the uptake and expression of these molecules in the target cells.
2
Effectene by Qiagen
Sourced in Germany, United States, United Kingdom, Spain, Netherlands, Switzerland, France
Effectene is a transfection reagent developed by Qiagen. It is designed to efficiently deliver DNA, RNA, or other molecules into eukaryotic cells for various research applications.
3
Effectene reagent by Qiagen
Sourced in United States, Germany, China
Effectene reagent is a transfection reagent designed for efficient delivery of nucleic acids into eukaryotic cells. It facilitates the uptake of DNA, RNA, or other macromolecules into cells, enabling various research applications.
4
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
5
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
6
Lipofectamine 2000 by Thermo Fisher Scientific
Sourced in United States, China, Germany, United Kingdom, Canada, Japan, France, Italy, Switzerland, Australia, Spain, Belgium, Denmark, Singapore, India, Netherlands, Sweden, New Zealand, Portugal, Poland, Israel, Lithuania, Hong Kong, Argentina, Ireland, Austria, Czechia, Cameroon, Taiwan, Province of China, Morocco
Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
7
Dual luciferase reporter assay system by Promega
Sourced in United States, China, Germany, United Kingdom, Switzerland, Japan, France, Italy, Spain, Austria, Australia, Hong Kong, Finland
The Dual-Luciferase Reporter Assay System is a laboratory tool designed to measure and compare the activity of two different luciferase reporter genes simultaneously. The system provides a quantitative method for analyzing gene expression and regulation in transfected or transduced cells.
8
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
9
Schneider s medium by Thermo Fisher Scientific
Sourced in United States, United Kingdom
Schneider's medium is a liquid culture medium used for the growth and maintenance of various types of cells, including insect cells. It is designed to provide the necessary nutrients and conditions for the optimal growth and proliferation of these cell lines. The composition of Schneider's medium is formulated to support the specific requirements of insect cell cultures.
10
Schneider s drosophila medium by Thermo Fisher Scientific
Sourced in United States, United Kingdom, France, China
Schneider's Drosophila medium is a specialized cell culture medium designed for the growth and maintenance of Drosophila cell lines. It provides the essential nutrients and growth factors required for the optimal cultivation of these insect-derived cells.

More about "Effectene"

Effectene is a powerful transfection reagent that enables efficient delivery of nucleic acids, such as plasmids or siRNA, into mammalian cells.
Its unique lipid-based formulation forms compact complexes with DNA, enhancing cellular uptake while minimizing cytotoxicity.
This makes Effectene a versatile tool for a wide range of molecular and cell biology applications, including gene expression studies, gene silencing experiments, and investigations into cellular processes and disease mechanisms.
Effectene is often compared to other popular transfection reagents like Lipofectamine 2000, and it offers several advantages.
Its high transfection efficiencies and ease of use make it a popular choice among researchers.
Effectene can be used in conjunction with various cell culture media, such as DMEM and Schneider's Drosophila medium, as well as supplemented with FBS and Penicillin/Streptomycin.
When conducting experiments with Effectene, researchers may also utilize the Dual-Luciferase Reporter Assay System to measure gene expression or gene silencing.
This streamlines the research process and helps enhance the reliability and reproducibility of findings.
For those looking to optimize their research workflows and improve the accuracy of their results, tools like PubCompare.ai can be invaluable.
This AI-powered platform helps researchers quickly locate relevant protocols from literature, preprints, and patents, and identify the best products and procedures using data-driven comparisons.