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Eicosanoids

Eicosanoids are a diverse class of bioactive lipid mediators derived from arachidonic acid and other polyunsaturated fatty acids.
They play crucial roles in a variety of physiological and pathological processes, including inflammation, immune response, cardiovascular function, and pain perception.
Eicosanoids encompass a broad range of compounds, such as prostaglandins, leukotrienes, and thromboxanes, each with distinct functions and signaling pathways.
Understanding the complex biology of eicosanoids is essential for developing effective therapies for a wide range of diseases, from asthma and arthritis to cardiovascular disorders and cancer.
Reasearch in this field requires reliable protocols and meticulous analysis to ensure reproducibilty and accuaracy, which is where PubCompare.ai can streamline your eicosanoids research.

Most cited protocols related to «Eicosanoids»

1
Comprehensive Biomarker Profiling in Disease Phenotypes
Cited 24 times
It was our objective to unravel and to validate laboratory biomarkers significantly associated with disease phenotypes and risks. An extensive panel of laboratory tests covering 83 analytes and biomarkers (clinical chemistry, hematology, immunology, endocrinology and metabolism) was performed on fresh biospecimen directly on the day of sample collection in a highly standardized manner (Table 4). It is a major goal to investigate and to identify novel genetic modifiers of phenotypes and disease risk. To this end, we aimed to genotype all participants using the Affymetrix AXIOM-CEU genome-wide SNP array, addressing a total of 587,352 variants. Genotyping was accompanied by genome-wide gene expression analyses for the whole blood, which was collected in Tempus Blood RNA Tubes (Life Technologies) and transferred to -80 °C prior to further processing. Isolated RNA was processed and hybridised to Illumina HT-12 v4 Expression BeadChips (Illumina, San Diego, CA, USA) and scanned on the Illumina HiScan. We investigated the association of metabolic biomarkers (quantitiative tandem mass spectrometry: amino acidy, fatty acid oxidation, steroids, sterols, eicosanoids, phospholipids, tri- and diacylglycerols, apolipoproteins and others) with major disease phenotypes, the genome and other biomarkers. For the whole blood analysis of 26 amino acids, free carnitine and 34 acylcarnitines, 40 μl of native EDTA whole blood were spotted on filter paper WS 903 (GE Healthcare, Germany). Dried blood spots were stored at -80 °C after 3 h of drying until batch analysis. Sample pretreatment and measurement are described in detail elsewhere [74 (link), 75 (link)]. Analyses were performed on an API 2000 and API 4000 tandem mass spectrometer (Applied Biosystems, Germany). Quantification was performed using ChemoView™ 1.4.2 software (Applied Biosystems, Germany).
In a subcohort of over 900 participants comprising all age groups, a detailed leukocyte subtype phenotyping with an extensive antibody panel was performed from EDTA whole blood samples using 10 colour flow cytometry (Navios flow cytometer, Beckman Coulter, Pasadena, CA, USA) [76 (link), 77 ].
Loeffler M., Engel C., Ahnert P., Alfermann D., Arelin K., Baber R., Beutner F., Binder H., Brähler E., Burkhardt R., Ceglarek U., Enzenbach C., Fuchs M., Glaesmer H., Girlich F., Hagendorff A., Häntzsch M., Hegerl U., Henger S., Hensch T., Hinz A., Holzendorf V., Husser D., Kersting A., Kiel A., Kirsten T., Kratzsch J., Krohn K., Luck T., Melzer S., Netto J., Nüchter M., Raschpichler M., Rauscher F.G., Riedel-Heller S.G., Sander C., Scholz M., Schönknecht P., Schroeter M.L., Simon J.C., Speer R., Stäker J., Stein R., Stöbel-Richter Y., Stumvoll M., Tarnok A., Teren A., Teupser D., Then F.S., Tönjes A., Treudler R., Villringer A., Weissgerber A., Wiedemann P., Zachariae S., Wirkner K, & Thiery J. (2015). The LIFE-Adult-Study: objectives and design of a population-based cohort study with 10,000 deeply phenotyped adults in Germany. BMC Public Health, 15, 691.
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Publication 2015
acylcarnitine Age Groups Amino Acids Apolipoproteins Biological Markers BLOOD Carnitine Diglycerides Edetic Acid Eicosanoids Exanthema Fatty Acids Flow Cytometry Gene Expression Profiling Genes, Modifier Genome Hematologic Tests Immunoglobulins Leukocytes Metabolism Phospholipids Radionuclide Imaging Specimen Collection Steroids Sterols System, Endocrine Tandem Mass Spectrometry
2
Lipid Autacoids Extraction and Analysis
Cited 11 times
Lipid autacoids were extracted by solid phase with SampliQ ODS-C18 cartridges (Agilent Technologies). Eicosanoids and docosanoids were identified and quantified using a triple-quadrupole linear ion trap LC/MS/MS system (MDS SCIEX 3200 QTRAP) equipped with a Kinetex C18 mini-bore column.
von Moltke J., Trinidad N.J., Moayeri M., Kintzer A.F., Wang S.B., van Rooijen N., Brown C.R., Krantz B.A., Leppla S.H., Gronert K, & Vance R.E. (2012). Rapid induction of inflammatory lipid mediators by the inflammasome in vivo. Nature, 490(7418), 107-111.
Publication 2012
Autacoids Eicosanoids Lipids Tandem Mass Spectrometry
3
Eicosanoid Analysis by LC-MS/MS
Cited 7 times
Eicosanoids were analyzed as detailed by the Lipid Maps Consortium33 (link),34 (link). Culture media (4 ml) from siRNA was combined with 10% methanol (400 μl) and glacial acetic acid (20 μl) before spiking with internal standard (100 μl) containing the following deuterated eicosanoids (100 pg/μl, 10 ng total): (d4) 6keto-PGF1α, (d4) PGF2α, (d4) PGE2, (d4) PGD2, (d8) 5-hydroxyeicosa-tetranoic acid (5HETE), (d8) 15-hydroxyeicosatetranoic acid (15HETE), (d8) 14,15 epoxyeicosa-trienoic acid and (d8) arachidonic acid. Samples and vial rinses (5% MeOH; 2 ml) were applied to Strata-X SPE columns (Phenomenex), previously washed with methanol (2 ml) and then dH2O (2 ml). Eicosanoids eluted with isopropanol (2 ml), were dried in vacuuo and reconstituted in EtOH:dH2O (50:50;100 μl) prior to HPLC ESI-MS/MS analysis (see Supplementary Methods).
Simanshu D.K., Kamlekar R.K., Wijesinghe D.S., Zou X., Zhai X., Mishra S.K., Molotkovsky J.G., Malinina L., Hinchcliffe E.H., Chalfant C.E., Brown R.E, & Patel D.J. (2013). Nonvesicular trafficking by a ceramide-1-phosphate transfer protein regulates eicosanoids. Nature, 500(7463), 463-467.
Publication 2013
Acetic Acid Acids Arachidonic Acid Culture Media Dinoprost Dinoprostone Eicosanoids Ethanol High-Performance Liquid Chromatographies Isopropyl Alcohol Lipids Methanol Microtubule-Associated Proteins PGF1alpha Prostaglandin D2 RNA, Small Interfering Tandem Mass Spectrometry
4
Eicosanoid and NAE Extraction Protocol
Cited 7 times

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Publication 2011
Connective Tissue Eicosanoids Lipids Methanol Neoplasm Metastasis S100 Proteins Solid Phase Extraction Tissues
5
LC-MS/MS Analysis of Eicosanoids in C. elegans
Cited 6 times

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Publication 2010
Caenorhabditis elegans Caimans Chromatography Eicosanoids formic acid High-Performance Liquid Chromatographies Strains Tandem Mass Spectrometry

Most recents protocols related to «Eicosanoids»

1
Quantitative PCR Analysis of Kidney Transcripts
Total RNA from kidneys was extracted using TissueLyser II (Qiagen, Germantown, MD) and a RNeasy Mini Kit (Qiagen; cat. #74104) according to the manufacturer’s instructions. Isolated RNA was reconstituted in RNase-free water and quantified using a Nanodrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA). cDNA was prepared from isolated RNA at a concentration of 100 ng/µl using a High-Capacity cDNA Reverse Transcriptase Kit (Thermo Fisher Scientific, Waltham, MA). Taqman assays were run with technical triplicates using a Smart Chip Real-Time PCR System at the MSU Genomics Core to assess interleukin (Il1a, Il1b, Il2, Il6, Il17a, Il18), chemokine (Ccl2, Ccl7, Ccl12, Cxcl9, Cxcl10, Cxcl13), inflammation/autoimmunity (C1qa, C3, Casp1, Casp4, Icam1, Ifng, Lbp, Nfkb1, Nlrp3, Nos2, Pparg, Tlr4, Tlr9, Tnfa, Tnfsf13b), type I interferon (IFN)-related (Ifi44, Irf7, Isg15, Nlrc5, Oas2), eicosanoid-related (Alox15, Cyp2c44, Cyp2j6, Cyp2j9, Cyp2j11, Ephx1, Ephx2, Pla2g4c, Ptgs2), kidney injury (Ankrd1, Cd14, Havcr1, Tgfbr1), oxidative stress-related (Hmox, Ncf1, Nqo1, Sod2), and housekeeping (Actb, Gusb) gene expression. Raw Ct values for each gene were converted to ΔCt values by subtracting the average Ct of the housekeeping genes from the Ct of the specified gene, and ΔΔCt values for each gene were calculated relative to the VEH/CON group by subtracting the average VEH/CON ΔCt value from individual ΔCt values within all experimental groups. The ΔΔCt values for each gene were then converted to relative copy number (RCN) values using the following equation (68 (link)):
Favor O.K., Chauhan P.S., Pourmand E., Edwards A.M., Wagner J.G., Lewandowski R.P., Heine L.K., Harkema J.R., Lee K.S, & Pestka J.J. (2023). Lipidome modulation by dietary omega-3 polyunsaturated fatty acid supplementation or selective soluble epoxide hydrolase inhibition suppresses rough LPS-accelerated glomerulonephritis in lupus-prone mice. Frontiers in Immunology, 14, 1124910.
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Publication 2023
ALOX15 protein, human Autoimmune Diseases Biological Assay CASP4 protein, human CCL2 protein, human CCL7 protein, human Chemokine Chemokine CXCL13 CXCL9 protein, human cytochrome P-450 CYP2J9 (murine) DNA, Complementary DNA Chips Eicosanoids Endoribonucleases EPHX1 protein, human Gene Expression Genes Genes, Housekeeping IL1A protein, human Inflammation Injuries Intercellular Adhesion Molecule-1 Interferon Type I Interferon Type II Interleukin-1 Interleukin-17A Interleukin-18 Interleukins IRF7 protein, human Kidney NAD(P)H dehydrogenase (quinone) 1, human NCF1 protein, human Nitric Oxide Synthase Type II Oxidative Stress PLA2G4C protein, human PTGS2 protein, human Receptor, Transforming Growth Factor-beta Type I RNA-Directed DNA Polymerase SOD2 protein, human TNF protein, human TNFSF13B protein, human
2
Eicosanoid Profiling in Zebrafish Larvae

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Publication 2023
Acetic Acid acetonitrile Buffers Chromatography DNA Replication Eicosanoids Isopropyl Alcohol Larva Lipids Mass Spectrometry Methanol Microtubule-Associated Proteins NR2F6 protein, human Polymers Solid Phase Extraction Zebrafish
3
Targeted Metabolomic Analysis of Signaling Metabolites
Metabolomic studies were performed using directed, non-targeted liquid chromatography-mass spectrometry (LC-MS) approaches to specifically capture and assay small polar “bioactive” metabolites. These were deemed to have a higher likelihood of interacting with cell surface receptors involved in signaling. These include free fatty acids, eicosanoids and oxylipins, bile acids, and fatty acid esters of hydroxy fatty acids, among hundreds of unidentified related metabolites [34 (link),35 (link)]. Metabolite levels are used as continuous traits with a mean of 0 and standard deviation of 1. Identified metabolites were confirmed through internal standards.
Granados J.C., Watrous J.D., Long T., Rosenthal S.B., Cheng S., Jain M, & Nigam S.K. (2023). Regulation of Human Endogenous Metabolites by Drug Transporters and Drug Metabolizing Enzymes: An Analysis of Targeted SNP-Metabolite Associations. Metabolites, 13(2), 171.
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Publication 2023
Bile Acids Biological Assay Eicosanoids Esters Fatty Acids Hydroxy Acids Liquid Chromatography Mass Spectrometry Nonesterified Fatty Acids Oxylipins Receptors, Cell Surface
4
Urine F2-Isoprostane Quantification
Urine samples were collected by either free catch or cystocentesis. After centrifugation (400 rcf for 5 min at room temperature), 1 mL aliquots were made and immediately frozen at −80 °C until analysis as a single batch. F2-IsoP (specifically the 15-F2t-isoprostane isomer) was quantified by liquid chromatography/mass spectroscopy at the Eicosanoid Core Laboratory at Vanderbilt University (Nashville, TN, USA), following methods based on their published protocol [21 (link)] and outlined in Supplementary File S2. Final F2-IsoP concentrations were normalized against urinary creatinine (UCr) concentrations and reported in units ng/mg UCr.
Phillips R.K., Steiner J.M., Suchodolski J.S, & Lidbury J.A. (2023). Urinary 15-F2t-Isoprostane Concentrations in Dogs with Liver Disease. Veterinary Sciences, 10(2), 82.
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Publication 2023
8-isoprostaglandin F2alpha Centrifugation Creatinine Eicosanoids Freezing Isomerism Liquid Chromatography Mass Spectrometry Urine
5
Phylogenetic Analysis of CYP and GST Enzyme Families
CYP protein sequences from A. thaliana and O. sativa [63 ,64 ] were used to perform BLASTP searches using a minimum E value cut-off of 1e-10 against the predicted proteomes of S. moellendorffii, M. polymorpha, A. agrestis, P. patens, K. nitens, C. reinhardtii, and C. merolae. GST protein sequences were retrieved by BLASTP searches using GST proteins from A. thaliana [65 (link),66 ], O. sativa [67 (link),68 (link)], and P. patens [28 (link)] against the predicted proteomes of S. moellendorffii, M. polymorpha, A. agrestis, K. nitens, C. reinhardtii, and C. merolae. This initial list of sequences for each species was used as a query for BLASTP searches against the proteome of that species to retrieve additional sequences belonging to species-specific clans. Each CYP sequence was checked for the presence of the cytochrome p450 domain (PF00067, IPR00128) and each GST sequence was checked for the presence of the GST N-terminal domain (IPR004045, IPR019564, PF13409, PF17172, PF13417 and PF02798) and C-terminal domain (IPR010987, PF13410, PF00043, PF14497 and PF17171) using InterProScan 84.0 [69 (link)].
Two enzyme families with glutathione transferase activity, kappa [70 (link)] and membrane associated proteins in eicosanoid and glutathione metabolism (MAPEG) [71 (link)], do not possess a GST N-terminal thioredoxin-like domain or GST C-terminal domain and lack the N-terminal active site found in all other GST proteins. An additional group of sequences was identified by this analysis possessing two GST N-terminal domains (2N) but lacking a C-terminal domain. Protein sequences belonging to the kappa, MAPEG, and 2N classes were therefore not included in the phylogenetic analysis but are listed in S5 Table.
Casey A, & Dolan L. (2023). Genes encoding cytochrome P450 monooxygenases and glutathione S-transferases associated with herbicide resistance evolved before the origin of land plants. PLOS ONE, 18(2), e0273594.
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Publication 2023
Amino Acid Sequence Cytochrome P450 Eicosanoids enzyme activity Glutathione Glutathione S-Transferase Membrane Proteins Metabolism Proteins Proteome Staphylococcal Protein A Thioredoxins

Top products related to «Eicosanoids»

1
Pge2 d4 by Cayman Chemical
Sourced in United States, Germany
PGE2-d4 is a stable-isotope labeled internal standard for the quantification of prostaglandin E2 (PGE2) by mass spectrometry. It serves as a reference compound to ensure accurate and reliable measurement of PGE2 levels in biological samples.
2
Eicosanoid standards by Cayman Chemical
Sourced in United States
Eicosanoid standards are reference materials used to identify and quantify eicosanoid compounds in analytical techniques such as chromatography and mass spectrometry. They provide a known, consistent composition to calibrate and validate analytical methods.
3
Enzyme immunoassay kit by Cayman Chemical
Sourced in United States
The Enzyme immunoassay kit is a laboratory instrument used to detect and quantify specific substances, such as proteins, hormones, or other analytes, in a sample. It utilizes the principle of antigen-antibody binding and an enzyme-based detection system to provide a sensitive and reliable measurement of the target analyte.
4
Ltb4 d4 by Cayman Chemical
Sourced in United States, Germany
LTB4-d4 is a deuterium-labeled leukotriene B4 analog. It is used as an internal standard in analytical methods for the quantification of leukotriene B4 levels in biological samples.
5
Sep pak c18 column by Waters Corporation
Sourced in United States, United Kingdom, Australia
The Sep-Pak C18 column is a solid-phase extraction (SPE) cartridge used for sample preparation in analytical chemistry. It contains a silica-based stationary phase with C18 (octadecyl) functional groups, which are effective for the retention and separation of a wide range of organic compounds. The column is designed to facilitate the extraction, purification, and concentration of analytes from complex sample matrices.
6
Qtrap 5500 mass spectrometer by AB Sciex
Sourced in United States, Canada, Japan, Germany, Austria, China, Switzerland
The QTRAP 5500 mass spectrometer is a high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. It is designed to provide sensitive and selective analysis of small molecules. The instrument features a triple quadrupole configuration and advanced ion optics for efficient ion transmission and fragmentation.
7
Formic acid by Merck Group
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Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.
8
Analyst 1 by AB Sciex
Sourced in United States, Canada, Germany, Singapore, United Kingdom
Analyst 1.6.3 software is a data processing and analysis tool developed by AB Sciex for use with their mass spectrometry instruments. The software provides functionalities for data acquisition, processing, and visualization. It serves as a core component in the operation and analysis of mass spectrometry experiments.
9
Multiquant software by AB Sciex
Sourced in United States, Canada
MultiQuant software is a data processing application designed for quantitative analysis of mass spectrometry data. It provides a comprehensive suite of tools for data review, peak detection, and quantification of target analytes in complex biological samples.
10
12 hete d8 by Cayman Chemical
Sourced in United States
12-HETE-d8 is a deuterated analogue of the lipid mediator 12-hydroxyeicosatetraenoic acid (12-HETE). It is used as an internal standard for the quantification of 12-HETE in analytical procedures.

More about "Eicosanoids"

Eicosanoids, a diverse class of bioactive lipid mediators, play pivotal roles in numerous physiological and pathological processes, including inflammation, immune response, cardiovascular function, and pain perception.
This broad family of compounds encompasses prostaglandins, leukotrienes, thromboxanes, and other metabolites derived from arachidonic acid and other polyunsaturated fatty acids.
Understanding the complex biology of eicosanoids is crucial for developing effective therapies for a wide range of diseases, from asthma and arthritis to cardiovascular disorders and cancer.
Reliable protocols and meticulous analysis are essential for eicosanoids research, as evidenced by the use of specialized tools like PGE2-d4 (a deuterated PGE2 standard), Eicosanoid standards, Enzyme immunoassay kits, LTB4-d4 (a deuterated leukotriene B4 standard), Sep-Pak C18 columns, and advanced mass spectrometry instruments like the QTRAP 5500.
Researchers in this field rely on analytical techniques such as liquid chromatography-tandem mass spectrometry (LC-MS/MS) with the aid of software like Analyst 1.6.3 and MultiQuant to ensure accurate quantification and identification of eicosanoid species.
Formic acid is commonly used as a mobile phase additive to improve chromatographic separation and ionization efficiency.
By streamlining eicosanoids research, PubCompare.ai empowers scientists to find the most reliable protocols, products, and methods, ultimately enhancing reproducibility and accuracy in their investigations of these crucial bioactive lipids and their diverse physiological and pathological roles.