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Epigallocatechin

Epigallocatechin is a flavanol compound found in green tea leaves.
It has demonstrated potent antioxidant, anti-inflammatory, and neuroprotective properties in preclinical studies.
Leveraging PubCompare.ai's AI-powered platform, researchers can explore the latest protocols and discover the most reliable and effective products for their epigallocatechin-related studies, enhacing reproducibility and research accuracy.
Expierience the future of scientific discovery today.

Most cited protocols related to «Epigallocatechin»

1
Compound-Target Interaction Analysis Protocol
Cited 17 times
For the above recorded abstracts, text mining was rescanned on them according to below rules:

Rule 1: ‘Compound name’ AND ‘any word in type A’

For instance, the sentence ‘(-)-Epigallocatechin-3-gallate is a novel Hsp90 inhibitor’ matches the rule well.

Rule 2: ‘Compound name’ AND ‘any word in type B interaction’ AND ‘any word in type B effect’

For example, the sentence ‘procyanidin B2 directly inhibited membrane type-1 (MT1)-MMP activity’ is a perfect match.

Manual check was done to all the abstracts being text mined to retrieve useful information into HIT.
Ye H., Ye L., Kang H., Zhang D., Tao L., Tang K., Liu X., Zhu R., Liu Q., Chen Y.Z., Li Y, & Cao Z. (2010). HIT: linking herbal active ingredients to targets. Nucleic Acids Research, 39(Database issue), D1055-D1059.
Publication 2010
epigallocatechin gallate HSP90 Heat-Shock Proteins procyanidin B2 Tissue, Membrane
2
Isolation and Culture of Human RPE Cells
Cited 10 times
The use of human material was approved by the Ethics Committee of the University of Leipzig and was performed according to the Declaration of Helsinki. Human RPE cells were obtained from several donors within 48 h of death, and were prepared and cultured as follows. After the vitreous and the retina were removed, the RPE cells were mechanically harvested, separated by digestion with 0.05% trypsin and 0.02% EDTA, and washed two times with PBS pH 7.2 (1.54 mM KH2PO4; 155.17 mM NaCl; 2.71 mM Na2HPO4x7H2O, Invitrogen, Paisley, UK). The cells were suspended in complete Ham F-10 medium containing 10% fetal bovine serum, GlutaMAX II, and penicillin/streptomycin, and were cultured in tissue culture flasks (Greiner, Nürtingen, Germany) in 95% air/5% CO2 at 37 °C. Cells of passages 3 to 5 were used. The epithelial nature of the RPE cells was routinely identified with immunocytochemistry using the monoclonal antibodies AE1 (recognizing most of the acidic type I keratins) and AE3 (recognizing most of the basic type II keratins), both from Chemicon. To test the substances, cultures that reached approximately 90% confluency were growth arrested in medium without serum for 5 h. Subsequently, media containing 0.5% serum with and without test substances were added.
Chen R., Hollborn M., Grosche A., Reichenbach A., Wiedemann P., Bringmann A, & Kohen L. (2014). Effects of the vegetable polyphenols epigallocatechin-3-gallate, luteolin, apigenin, myricetin, quercetin, and cyanidin in primary cultures of human retinal pigment epithelial cells. Molecular Vision, 20, 242-258.
Publication 2014
Acids Cells Digestion Donors Edetic Acid Epithelial Cells Ethics Committees Fetal Bovine Serum Homo sapiens Immunocytochemistry Keratins, Acidic Keratins, Type II Monoclonal Antibodies Penicillins Retina Serum Sodium Chloride Streptomycin Tissues Trypsin
3
Cell Growth Analysis by Hoechst 33258
Cited 7 times

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Publication 2008
Cell Proliferation Fluorescent Dyes Hoechst 33258
4
Evaluating Intracellular ROS Levels
Cited 6 times
To evaluate intracellular ROS levels, dichlorodihydrofluorescein diacetate (DCFH-DA) assay was performed as previously described [22 (link)]. Cells were seeded in a 96-well plate at density of 10000 cells/cm2, 4 replicates for each condition, treated with EGCG and SF for 72 hours soon after thawing, otherwise chronically during expansions. Cell culture medium was removed, and the 5 μM DCFH-DA was incubated in αMEM 1% FBS without phenol red for 30 min, at 37°C and 5% CO2. The cell culture plate was washed with PBS, and fluorescence of the cells was read at 485 nm (excitation) and 535 nm (emission) using the VICTOR multilabel plate reader (PerkinElmer).
Marrazzo P., Angeloni C., Freschi M., Lorenzini A., Prata C., Maraldi T, & Hrelia S. (2018). Combination of Epigallocatechin Gallate and Sulforaphane Counteracts In Vitro Oxidative Stress and Delays Stemness Loss of Amniotic Fluid Stem Cells. Oxidative Medicine and Cellular Longevity, 2018, 5263985.
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Publication 2018
Biological Assay Cell Culture Techniques Cells Culture Media epigallocatechin gallate Fluorescence Protoplasm
5
Dietary EGCG Effects on Obesity and Metabolism
Cited 6 times
All animal experiments were conducted according to protocols approved by the Institutional Animal Care and Use Committee at Rutgers University (Piscataway, NJ). One hundred male C57BL/6J mice (age 5−6 wk) from either the departmental breeding colony or Jackson Laboratories were used in 2 long-term experiments. The first long-term treatment (Expt. 1, n = 38, 6 wk old) was designed to determine the effects of EGCG on BW and body fat in high-fat–fed mice. The 2nd long-term treatment (Expt. 2, n = 56, 5 wk old) was designed to confirm the findings on BW and fat from Expt. 1 and also to determine the effects of EGCG on fecal lipids, hyperglycemia, insulin resistance, markers of inflammation, and fatty liver caused by a high-fat diet. In both studies, mice were fed a LF, HF, or HFE diet for 16 wk. Based on allometric scaling, the presently used dose of 3.2 g/kg diet in mice corresponds to 10 200-mL cups of green tea (containing 2 g tea leaves per cup) per day for an average person requiring 8374 kJ (2000 kcal)/d. BW and food intake (on a per-cage basis) were measured weekly. For fecal lipid measurements from Expt. 2, cage bedding was changed during wk 10 and feces were collected from the cages the following day.
Bose M., Lambert J.D., Ju J., Reuhl K.R., Shapses S.A, & Yang C.S. (2008). The Major Green Tea Polyphenol, (−)-Epigallocatechin-3-Gallate, Inhibits Obesity, Metabolic Syndrome, and Fatty Liver Disease in High-Fat–Fed Mice,. The Journal of nutrition, 138(9), 1677-1683.
Publication 2008
Body Fat Diet, High-Fat Eating epigallocatechin gallate Feces Green Tea Hyperglycemia Inflammation Institutional Animal Care and Use Committees Insulin Resistance Lipids Long-Term Care Males Mice, House Mice, Inbred C57BL Steatohepatitis Therapy, Diet

Most recents protocols related to «Epigallocatechin»

1
Anesthesia and Drug Administration Protocol
Not available on PMC !
Anesthesia was administered using ketamine hydrochloride (Ketalar, 100 mg/kg, Pfizer İlaçları Ltd., İstanbul, Türkiye) and xylazine hydrochloride (Rompun, 10 mg/ kg, Bayer, USA). Analgesia was administered using fentanyl citrate (Talinat, 0.5 mg/10 ml, Vem Pharmaceutical Industry Inc., Ankara, Türkiye). Cisplatin DBL 1 mg/mL was obtained from Orna İlac Tekstil Kimyevi Mad. San. ve Dıs. Tic. Ltd. Sti. (Beykoz/İstanbul, Türkiye), while infliximab (Remicade 100 mg/vial) was obtained from Merck Sharp Dohme Pharma Ltd. Epigallocatechin 3-gallate ((-)-epigallocatechin gallate, E4143-50MG) and all chemicals used in laboratory experiments were provided by Sigma-Aldrich Chemical Co. and Merck (Germany).
Ciftel E., Mercantepe F., Mercantepe T., Akyildiz K., Yilmaz A, & Ciftel S. (2024). Comparative Analysis of Epigallocatechin-3-Gallate and TNF-Alpha Inhibitors in Mitigating Cisplatin-Induced Pancreatic Damage Through Oxidative Stress and Apoptosis Pathways. Biological trace element research.
Publication 2024
2
Chitosan-EGCG Nanocomposite Synthesis
(−)-Epigallocatechin gallate (purity ≥95%), NaHCO3 (AR), NaOH (AR), and ZnCl2 (AR) were supplied by Macklin. Chitosan (50 kDa, 80–90% degree of deacetylation) was purchased from Rhawn. 2,2-diphenyl-1-picrylhydrazyl (DPPH) was purchased from Tokyo Chemical Industry. Methanol, ethanol, zinc acetate, and NaBH4 were of analytical grade and obtained from Aladdin. A dialysis bag (MWCO 8000–14000 Da) was purchased from Shanghai Yuanye Biotechnology Co., Ltd. All the reagents were applied without further treatment. E. coli (ATCC 25922) and S. aureus (ATCC 43300) were used for the antibacterial assays.
Zhao J., Qian D., Zhang L., Wang X, & Zhang J. (2024). Improved antioxidative and antibacterial activity of epigallocatechin gallate derivative complexed by zinc cations and chitosan. RSC Advances, 14(15), 10410-10415.
Publication 2024
3
Quantification of EPSF Compounds in White Tea
The quantification of EPSF compounds in white tea samples was carried out using the external standard method. This involved quantifying the following compounds: 5″ S-epicatechin-C N-ethyl-2-pyrrolidinone (S-EC-cThea), 5″ R-epicatechin-C N-ethyl-2-pyrrolidinone (R-EC-cThea), 5″ S-epigallocatechin-C N-ethyl-2-pyrrolidinone (S-EGC-cThea), 5″ R-epigallocatechin-C N-ethyl-2-pyrrolidinone (R-EGC-cThea), 5‴ S-epicatechin gallate-C N-ethyl-2-pyrrolidinone (S-ECG-cThea), 5‴ R-epicatechin gallate-C N-ethyl-2-pyrrolidinone (R-ECG-cThea), 5‴ S-epigallocatechin gallate-C N-ethyl-2-pyrrolidinone (S-EGCG-cThea), and 5‴ R-epigallocatechin gallate-8-C N-ethyl-2-pyrrolidinone (8-C R-EGCG-cThea) by calibration curve of 8-C R-EGCG-cThea. The calibration curve for 8-C R-EGCG-cThea was established using standard solutions of 0.01, 0.05, 0.1, 0.2, 0.5, and 1.0 mg/mL.
Chen Z., Dai W., Xiong M., Gao J., Zhou H., Chen D, & Li Y. (2024). Metabolomics investigation of the chemical variations in white teas with different producing areas and storage durations. Food Chemistry: X, 21, 101127.
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Publication 2024
4
Quantitative Determination of Catechins

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Publication 2024
5
EGCG Inhibits Glutamate Release in CPEKs
EGCG is a catechin found in green tea that has also been reported to inhibit glutamate dehydrogenase (GDH). To confirm the effect of YKS, we determined whether EGCG with GDH inhibitory effect exhibits a similar effect (Li et al., 2006 (link); Moon et al., 2007 (link); Li et al., 2011 (link); Li et al., 2012 (link)) by replicating the experiments described in the subsection titled “Effect of YKS on glutamate release from CPEK.”
CPEKs were cultured in a 48-well plate until reaching subconfluency. Once the CPEKs reached a certain density, the cells were washed twice with PBS, replaced with basal medium supplemented with different concentrations of EGCG (0, 10, 50, and 100 µM), and then incubated in a CO2 incubator at 37°C. After 24 hours of incubation, glutamate in the medium was measured using the glutamate assay kit and determined as the amount released from the cells over 24 hours.
Kasuga Y., Hu A., Kawakami Z., Tabuchi M., Yamaguchi T., Kobayashi H, & Ikeda S. (2024). Suppressive effect of Yokukansan on glutamate released from canine keratinocytes. Open Veterinary Journal, 14(2), 683-691.
Publication 2024

Top products related to «Epigallocatechin»

1
Epigallocatechin gallate (egcg) by Merck Group
Sourced in United States, Germany, China, Sao Tome and Principe, Spain, France, Canada, United Kingdom, Macao, India, Italy, Australia, Belgium, Israel, Denmark, Japan
EGCG is a laboratory reagent used in research applications. It is a bioactive compound extracted from green tea leaves. EGCG has demonstrated potential antioxidant and anti-inflammatory properties in scientific studies.
2
Gallic acid by Merck Group
Sourced in United States, Germany, Italy, Spain, France, India, China, Poland, Australia, United Kingdom, Sao Tome and Principe, Brazil, Chile, Ireland, Canada, Singapore, Switzerland, Malaysia, Portugal, Mexico, Hungary, New Zealand, Belgium, Czechia, Macao, Hong Kong, Sweden, Argentina, Cameroon, Japan, Slovakia, Serbia
Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.
3
Epicatechin by Merck Group
Sourced in United States, Germany, Italy, France, China, Spain, United Kingdom, Australia, Switzerland, Belgium, Sao Tome and Principe, India, New Zealand
Epicatechin is a natural compound found in various plants and is commonly used in laboratory settings. It serves as a standard reference material for analytical and research purposes. Epicatechin exhibits antioxidant properties and is often employed in the evaluation of antioxidant activity and the development of analytical methods.
4
Catechin by Merck Group
Sourced in United States, Germany, Italy, France, Australia, India, Spain, United Kingdom, China, Poland, Sao Tome and Principe, Japan, Portugal, Canada, Switzerland, Brazil, Malaysia, Singapore, Macao, Belgium, Ireland, Mexico, Hungary
Catechin is a natural polyphenolic compound found in various plants, including green tea. It functions as an antioxidant, with the ability to scavenge free radicals and protect cells from oxidative stress.
5
Quercetin by Merck Group
Sourced in United States, Germany, Italy, India, Spain, United Kingdom, France, Poland, China, Sao Tome and Principe, Australia, Brazil, Macao, Switzerland, Canada, Chile, Japan, Singapore, Ireland, Mexico, Portugal, Sweden, Malaysia, Hungary
Quercetin is a natural compound found in various plants, including fruits and vegetables. It is a type of flavonoid with antioxidant properties. Quercetin is often used as a reference standard in analytical procedures and research applications.
6
Epigallocatechin by Merck Group
Sourced in United States, Germany, United Kingdom, China, France
Epigallocatechin is a chemical compound found in green tea leaves. It is a type of flavonoid and has antioxidant properties. Epigallocatechin can be used as a reference standard in various analytical and research applications.
7
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
8
Epicatechin gallate by Merck Group
Sourced in United States, China, Germany, United Kingdom
Epicatechin gallate is a lab equipment product manufactured by Merck Group. It is a bioactive compound derived from green tea that can be used for various research and analytical applications. The core function of epicatechin gallate is to serve as a reference standard or analytical tool in scientific investigations.
9
Caffeic acid by Merck Group
Sourced in United States, Germany, Italy, France, Poland, Spain, China, United Kingdom, Australia, Sao Tome and Principe, Switzerland, India, Ireland, Canada, Macao, Brazil, Austria, Mexico, Czechia, Portugal
Caffeic acid is a phenolic compound commonly found in various plants. It serves as a laboratory standard for the identification and quantification of similar phenolic compounds using analytical techniques such as high-performance liquid chromatography (HPLC) and spectrophotometry.
10
Formic acid by Merck Group
Sourced in Germany, United States, Italy, United Kingdom, France, Spain, China, Poland, India, Switzerland, Sao Tome and Principe, Belgium, Australia, Canada, Ireland, Macao, Hungary, Czechia, Netherlands, Portugal, Brazil, Singapore, Austria, Mexico, Chile, Sweden, Bulgaria, Denmark, Malaysia, Norway, New Zealand, Japan, Romania, Finland, Indonesia
Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.

More about "Epigallocatechin"

Epigallocatechin, a powerful flavanol compound found in green tea leaves, has garnered significant attention for its remarkable antioxidant, anti-inflammatory, and neuroprotective properties.
Also known as EGCG, this natural compound has been extensively studied for its potential therapeutic applications.
Closely related to other polyphenols like gallic acid, epicatechin, catechin, and quercetin, epigallocatechin shares many of their beneficial effects.
These compounds, collectively referred to as catechins, are known for their ability to scavenge free radicals, reduce inflammation, and protect neuronal cells from damage.
Leveraging the cutting-edge AI-powered platform of PubCompare.ai, researchers can now explore the latest protocols and discover the most reliable and effective products for their epigallocatechin-related studies.
This innovative tool enhances reproducibility and research accuracy by facilitating the identification of the most robust and well-validated experimental approaches.
Whether you're investigating the potential of epigallocatechin in combating oxidative stress, modulating inflammatory pathways, or promoting neuronal health, PubCompare.ai's comprehensive database of scientific literature, preprints, and patents can provide invaluable insights.
By empowering researchers to make informed decisions about the most suitable reagents, cell lines, and experimental conditions, this platform helps to elevate the quality and impact of epigallocatechin-focused research.
Experience the future of scientific discovery today and unlock the full potential of this remarkable green tea constituent with the help of PubCompare.ai's AI-driven platform.