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Epigallocatechin gallate

Epigallocatechin gallate (EGCG) is a polyphenol compound found primarily in green tea.
It has been the subject of extensive research for its potential health benefits, including antioxidant, anti-inflammatory, and anti-cancer properties.
EGCG may also play a role in regulating metabolism and improving cardiovascular health.
However, optimizing EGCG research can be challening due to the complexiety of its pharmacokinetics and the need to identify the best protocols and products.
PubCompare.ai, the leading AI-driiven platform, can help enhace the reproducibility and accuracy of EGCG research by providing access to protocols from literature, pre-prints, and patents, and leveraging AI-driven comparisons to identify the most effective approaches.
This data-driven decision making can unlock the full potential of EGCG for various therapeutic and nutraceutical applications.

Most cited protocols related to «Epigallocatechin gallate»

1
Compound-Target Interaction Analysis Protocol
Cited 17 times
For the above recorded abstracts, text mining was rescanned on them according to below rules:

Rule 1: ‘Compound name’ AND ‘any word in type A’

For instance, the sentence ‘(-)-Epigallocatechin-3-gallate is a novel Hsp90 inhibitor’ matches the rule well.

Rule 2: ‘Compound name’ AND ‘any word in type B interaction’ AND ‘any word in type B effect’

For example, the sentence ‘procyanidin B2 directly inhibited membrane type-1 (MT1)-MMP activity’ is a perfect match.

Manual check was done to all the abstracts being text mined to retrieve useful information into HIT.
Ye H., Ye L., Kang H., Zhang D., Tao L., Tang K., Liu X., Zhu R., Liu Q., Chen Y.Z., Li Y, & Cao Z. (2010). HIT: linking herbal active ingredients to targets. Nucleic Acids Research, 39(Database issue), D1055-D1059.
Publication 2010
epigallocatechin gallate HSP90 Heat-Shock Proteins procyanidin B2 Tissue, Membrane
2
Isolation and Culture of Human RPE Cells
Cited 10 times
The use of human material was approved by the Ethics Committee of the University of Leipzig and was performed according to the Declaration of Helsinki. Human RPE cells were obtained from several donors within 48 h of death, and were prepared and cultured as follows. After the vitreous and the retina were removed, the RPE cells were mechanically harvested, separated by digestion with 0.05% trypsin and 0.02% EDTA, and washed two times with PBS pH 7.2 (1.54 mM KH2PO4; 155.17 mM NaCl; 2.71 mM Na2HPO4x7H2O, Invitrogen, Paisley, UK). The cells were suspended in complete Ham F-10 medium containing 10% fetal bovine serum, GlutaMAX II, and penicillin/streptomycin, and were cultured in tissue culture flasks (Greiner, Nürtingen, Germany) in 95% air/5% CO2 at 37 °C. Cells of passages 3 to 5 were used. The epithelial nature of the RPE cells was routinely identified with immunocytochemistry using the monoclonal antibodies AE1 (recognizing most of the acidic type I keratins) and AE3 (recognizing most of the basic type II keratins), both from Chemicon. To test the substances, cultures that reached approximately 90% confluency were growth arrested in medium without serum for 5 h. Subsequently, media containing 0.5% serum with and without test substances were added.
Chen R., Hollborn M., Grosche A., Reichenbach A., Wiedemann P., Bringmann A, & Kohen L. (2014). Effects of the vegetable polyphenols epigallocatechin-3-gallate, luteolin, apigenin, myricetin, quercetin, and cyanidin in primary cultures of human retinal pigment epithelial cells. Molecular Vision, 20, 242-258.
Publication 2014
Acids Cells Digestion Donors Edetic Acid Epithelial Cells Ethics Committees Fetal Bovine Serum Homo sapiens Immunocytochemistry Keratins, Acidic Keratins, Type II Monoclonal Antibodies Penicillins Retina Serum Sodium Chloride Streptomycin Tissues Trypsin
3
Cell Growth Analysis by Hoechst 33258
Cited 7 times

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Publication 2008
Cell Proliferation Fluorescent Dyes Hoechst 33258
4
Evaluating Intracellular ROS Levels
Cited 6 times
To evaluate intracellular ROS levels, dichlorodihydrofluorescein diacetate (DCFH-DA) assay was performed as previously described [22 (link)]. Cells were seeded in a 96-well plate at density of 10000 cells/cm2, 4 replicates for each condition, treated with EGCG and SF for 72 hours soon after thawing, otherwise chronically during expansions. Cell culture medium was removed, and the 5 μM DCFH-DA was incubated in αMEM 1% FBS without phenol red for 30 min, at 37°C and 5% CO2. The cell culture plate was washed with PBS, and fluorescence of the cells was read at 485 nm (excitation) and 535 nm (emission) using the VICTOR multilabel plate reader (PerkinElmer).
Marrazzo P., Angeloni C., Freschi M., Lorenzini A., Prata C., Maraldi T, & Hrelia S. (2018). Combination of Epigallocatechin Gallate and Sulforaphane Counteracts In Vitro Oxidative Stress and Delays Stemness Loss of Amniotic Fluid Stem Cells. Oxidative Medicine and Cellular Longevity, 2018, 5263985.
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Publication 2018
Biological Assay Cell Culture Techniques Cells Culture Media epigallocatechin gallate Fluorescence Protoplasm
5
Dietary EGCG Effects on Obesity and Metabolism
Cited 6 times
All animal experiments were conducted according to protocols approved by the Institutional Animal Care and Use Committee at Rutgers University (Piscataway, NJ). One hundred male C57BL/6J mice (age 5−6 wk) from either the departmental breeding colony or Jackson Laboratories were used in 2 long-term experiments. The first long-term treatment (Expt. 1, n = 38, 6 wk old) was designed to determine the effects of EGCG on BW and body fat in high-fat–fed mice. The 2nd long-term treatment (Expt. 2, n = 56, 5 wk old) was designed to confirm the findings on BW and fat from Expt. 1 and also to determine the effects of EGCG on fecal lipids, hyperglycemia, insulin resistance, markers of inflammation, and fatty liver caused by a high-fat diet. In both studies, mice were fed a LF, HF, or HFE diet for 16 wk. Based on allometric scaling, the presently used dose of 3.2 g/kg diet in mice corresponds to 10 200-mL cups of green tea (containing 2 g tea leaves per cup) per day for an average person requiring 8374 kJ (2000 kcal)/d. BW and food intake (on a per-cage basis) were measured weekly. For fecal lipid measurements from Expt. 2, cage bedding was changed during wk 10 and feces were collected from the cages the following day.
Bose M., Lambert J.D., Ju J., Reuhl K.R., Shapses S.A, & Yang C.S. (2008). The Major Green Tea Polyphenol, (−)-Epigallocatechin-3-Gallate, Inhibits Obesity, Metabolic Syndrome, and Fatty Liver Disease in High-Fat–Fed Mice,. The Journal of nutrition, 138(9), 1677-1683.
Publication 2008
Body Fat Diet, High-Fat Eating epigallocatechin gallate Feces Green Tea Hyperglycemia Inflammation Institutional Animal Care and Use Committees Insulin Resistance Lipids Long-Term Care Males Mice, House Mice, Inbred C57BL Steatohepatitis Therapy, Diet

Most recents protocols related to «Epigallocatechin gallate»

1
Anesthesia and Drug Administration Protocol
Not available on PMC !
Anesthesia was administered using ketamine hydrochloride (Ketalar, 100 mg/kg, Pfizer İlaçları Ltd., İstanbul, Türkiye) and xylazine hydrochloride (Rompun, 10 mg/ kg, Bayer, USA). Analgesia was administered using fentanyl citrate (Talinat, 0.5 mg/10 ml, Vem Pharmaceutical Industry Inc., Ankara, Türkiye). Cisplatin DBL 1 mg/mL was obtained from Orna İlac Tekstil Kimyevi Mad. San. ve Dıs. Tic. Ltd. Sti. (Beykoz/İstanbul, Türkiye), while infliximab (Remicade 100 mg/vial) was obtained from Merck Sharp Dohme Pharma Ltd. Epigallocatechin 3-gallate ((-)-epigallocatechin gallate, E4143-50MG) and all chemicals used in laboratory experiments were provided by Sigma-Aldrich Chemical Co. and Merck (Germany).
Ciftel E., Mercantepe F., Mercantepe T., Akyildiz K., Yilmaz A, & Ciftel S. (2024). Comparative Analysis of Epigallocatechin-3-Gallate and TNF-Alpha Inhibitors in Mitigating Cisplatin-Induced Pancreatic Damage Through Oxidative Stress and Apoptosis Pathways. Biological trace element research.
Publication 2024
2
Quantitative Determination of Catechins

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Publication 2024
3
Chitosan-EGCG Nanocomposite Synthesis
(−)-Epigallocatechin gallate (purity ≥95%), NaHCO3 (AR), NaOH (AR), and ZnCl2 (AR) were supplied by Macklin. Chitosan (50 kDa, 80–90% degree of deacetylation) was purchased from Rhawn. 2,2-diphenyl-1-picrylhydrazyl (DPPH) was purchased from Tokyo Chemical Industry. Methanol, ethanol, zinc acetate, and NaBH4 were of analytical grade and obtained from Aladdin. A dialysis bag (MWCO 8000–14000 Da) was purchased from Shanghai Yuanye Biotechnology Co., Ltd. All the reagents were applied without further treatment. E. coli (ATCC 25922) and S. aureus (ATCC 43300) were used for the antibacterial assays.
Zhao J., Qian D., Zhang L., Wang X, & Zhang J. (2024). Improved antioxidative and antibacterial activity of epigallocatechin gallate derivative complexed by zinc cations and chitosan. RSC Advances, 14(15), 10410-10415.
Publication 2024
4
Quantification of EPSF Compounds in White Tea
The quantification of EPSF compounds in white tea samples was carried out using the external standard method. This involved quantifying the following compounds: 5″ S-epicatechin-C N-ethyl-2-pyrrolidinone (S-EC-cThea), 5″ R-epicatechin-C N-ethyl-2-pyrrolidinone (R-EC-cThea), 5″ S-epigallocatechin-C N-ethyl-2-pyrrolidinone (S-EGC-cThea), 5″ R-epigallocatechin-C N-ethyl-2-pyrrolidinone (R-EGC-cThea), 5‴ S-epicatechin gallate-C N-ethyl-2-pyrrolidinone (S-ECG-cThea), 5‴ R-epicatechin gallate-C N-ethyl-2-pyrrolidinone (R-ECG-cThea), 5‴ S-epigallocatechin gallate-C N-ethyl-2-pyrrolidinone (S-EGCG-cThea), and 5‴ R-epigallocatechin gallate-8-C N-ethyl-2-pyrrolidinone (8-C R-EGCG-cThea) by calibration curve of 8-C R-EGCG-cThea. The calibration curve for 8-C R-EGCG-cThea was established using standard solutions of 0.01, 0.05, 0.1, 0.2, 0.5, and 1.0 mg/mL.
Chen Z., Dai W., Xiong M., Gao J., Zhou H., Chen D, & Li Y. (2024). Metabolomics investigation of the chemical variations in white teas with different producing areas and storage durations. Food Chemistry: X, 21, 101127.
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Publication 2024
5
Comprehensive Profiling of Tea Polyphenols
Dimeric catechins, such as theaflavin-3ʹ-gallate (TF-3ʹ-G), theaflavin-3,3ʹ-digallate (TF-DG), theaflavin-3-gallate (TF-3-G), theaflavin (TF), procyanidin B2, and procyanidin B1, were sourced from ChemFaces (Wuhan, Hubei, China). Additionally, the following compounds were procured from Sigma (St. Louis, MO, USA): nine flavanols, namely (−)-epigallocatechin (EGC), (−)-epicatechin (EC), (−)-gallocatechin gallate (GCG), (−)-epigallocatechin gallate (EGCG), (+)-catechin (C), (−)-gallocatechin (GC), (−)-epicatechin gallate (ECG), (−)-epigallocatechin-3-(4′'-O-methyl) gallate (EGCG-4′'-O-Me), and (−)-epiafzelechin 3-gallate; fifteen amino acids; eight kaempferol-O-glycosides and quercetin-O-glycosides; as well as flavone-C-glycosides, including vitexin and isovitexin. Other chemicals such as 1-ethyl-5-hydroxy-2-pyrrolidinone, quinic acid, benzyl primeveroside, pyroglutamic acid, pipecolic acid, choline, caffeine, and adenosine monophosphate (AMP), were procured from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Milli-Q water was used in this study (Millipore, Billerica, MA, USA). Liquid chromatography-mass spectrometry (LC-MS) grade formic acid, methanol, and acetonitrile of were obtained from Merck (Darmstadt, Germany) (Gao et al., 2022 (link), Gao et al., 2022 (link), Peng et al., 2021 (link)).
Chen Z., Dai W., Xiong M., Gao J., Zhou H., Chen D, & Li Y. (2024). Metabolomics investigation of the chemical variations in white teas with different producing areas and storage durations. Food Chemistry: X, 21, 101127.
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Publication 2024

Top products related to «Epigallocatechin gallate»

1
Epigallocatechin gallate (egcg) by Merck Group
Sourced in United States, Germany, China, Sao Tome and Principe, Spain, France, Canada, United Kingdom, Macao, India, Italy, Australia, Belgium, Israel, Denmark, Japan
EGCG is a laboratory reagent used in research applications. It is a bioactive compound extracted from green tea leaves. EGCG has demonstrated potential antioxidant and anti-inflammatory properties in scientific studies.
2
Gallic acid by Merck Group
Sourced in United States, Germany, Italy, Spain, France, India, China, Poland, Australia, United Kingdom, Sao Tome and Principe, Brazil, Chile, Ireland, Canada, Singapore, Switzerland, Malaysia, Portugal, Mexico, Hungary, New Zealand, Belgium, Czechia, Macao, Hong Kong, Sweden, Argentina, Cameroon, Japan, Slovakia, Serbia
Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.
3
Epicatechin by Merck Group
Sourced in United States, Germany, Italy, France, China, Spain, United Kingdom, Australia, Switzerland, Belgium, Sao Tome and Principe, India, New Zealand
Epicatechin is a natural compound found in various plants and is commonly used in laboratory settings. It serves as a standard reference material for analytical and research purposes. Epicatechin exhibits antioxidant properties and is often employed in the evaluation of antioxidant activity and the development of analytical methods.
4
Catechin by Merck Group
Sourced in United States, Germany, Italy, France, Australia, India, Spain, United Kingdom, China, Poland, Sao Tome and Principe, Japan, Portugal, Canada, Switzerland, Brazil, Malaysia, Singapore, Macao, Belgium, Ireland, Mexico, Hungary
Catechin is a natural polyphenolic compound found in various plants, including green tea. It functions as an antioxidant, with the ability to scavenge free radicals and protect cells from oxidative stress.
5
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
6
Quercetin by Merck Group
Sourced in United States, Germany, Italy, India, Spain, United Kingdom, France, Poland, China, Sao Tome and Principe, Australia, Brazil, Macao, Switzerland, Canada, Chile, Japan, Singapore, Ireland, Mexico, Portugal, Sweden, Malaysia, Hungary
Quercetin is a natural compound found in various plants, including fruits and vegetables. It is a type of flavonoid with antioxidant properties. Quercetin is often used as a reference standard in analytical procedures and research applications.
7
Epigallocatechin by Merck Group
Sourced in United States, Germany, United Kingdom, China, France
Epigallocatechin is a chemical compound found in green tea leaves. It is a type of flavonoid and has antioxidant properties. Epigallocatechin can be used as a reference standard in various analytical and research applications.
8
Epicatechin gallate by Merck Group
Sourced in United States, China, Germany, United Kingdom
Epicatechin gallate is a lab equipment product manufactured by Merck Group. It is a bioactive compound derived from green tea that can be used for various research and analytical applications. The core function of epicatechin gallate is to serve as a reference standard or analytical tool in scientific investigations.
9
Dimethyl sulfoxide (dmso) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
10
Trizol reagent by Thermo Fisher Scientific
Sourced in United States, China, Japan, Germany, United Kingdom, Canada, France, Italy, Australia, Spain, Switzerland, Netherlands, Belgium, Lithuania, Denmark, Singapore, New Zealand, India, Brazil, Argentina, Sweden, Norway, Austria, Poland, Finland, Israel, Hong Kong, Cameroon, Sao Tome and Principe, Macao, Taiwan, Province of China, Thailand
TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.

More about "Epigallocatechin gallate"

Epigallocatechin gallate (EGCG) is a powerful polyphenol compound found abundantly in green tea.
It has been extensively researched for its remarkable health benefits, including potent antioxidant, anti-inflammatory, and anti-cancer properties.
EGCG may also play a crucial role in regulating metabolism and improving cardiovascular health.
Gallic acid, a closely related compound, is another polyphenol found in green tea, with similar health-promoting effects.
Epicatechin and catechin are additional flavonoids present in green tea, also known for their antioxidant and therapeutic potential.
Optimizing EGCG research can be challenging due to the complexity of its pharmacokinetics and the need to identify the most effective protocols and products.
PubCompare.ai, the leading AI-driven platform, can enhance the reproducibility and accuracy of EGCG research by providing access to a wealth of protocols from literature, pre-prints, and patents, and leveraging AI-driven comparisons to identify the optimal approaches.
This data-driven decision making can unlock the full potential of EGCG for various therapeutic and nutraceutical applications, from cancer prevention and treatment to metabolic regulation and cardiovascular health.
Quercetin, epigallocatechin, and epicatechin gallate are other polyphenolic compounds found in green tea that may synergistically contribute to the beneficial effects of EGCG.
Researchers can utilize PubCompare.ai to explore the impact of factors like FBS, DMSO, and TRIzol reagent on EGCG efficacy, ensuring more robust and reliable findings.
By harnessing the power of this AI-driven platform, scientists can accelerate the advancement of EGCG research and unlock new frontiers in green tea-derived therapeutic and nutraceutical applications.