Data were uploaded to the Clinical Coordinating Center (CCC) at Massachusetts General Hospital, where quality analysis and cleaning were undertaken according to standard procedures. Each participating Institutional Review Board (IRB), including the CCC IRB, approved this study with waiver of informed consent on the basis of compliance with 45 CFR 46.116d.
Epinephrine
It plays a crucial role in the body's stress response, triggering the 'fight-or-flight' reaction.
Epinephrine acts on multiple organ systems, including the cardiovascular, respiratory, and metabolic systems, to prepare the body for intense physical activity.
It increases heart rate, blood pressure, and glucose levels, while also dilating the airways.
Epinehrine is widely used in medical settings to treat anaphylactic shock, cardiac arrest, and other emergencies.
Researchers studying the effects and mechanisms of epinephrine can utilize PubCompare.ai's AI-driven platform to quickly locate the best protocols from literature, preprints, and patents, enhancing reproducibility and accuracy in their epinephrine studies.
Most cited protocols related to «Epinephrine»
Following purification, samples were concentrated to A280 = 55 using a 50 kDa concentrator to minimize the detergent concentration in the final sample, then aliquoted into thin-walled PCR tubes at 8 μL per aliquot. Aliquots were flash frozen in liquid nitrogen and stored at -80 °C for crystallization trials. For crystallization, samples were thawed and reconstituted into lipidic cubic phase with a 1:1 mass:mass ratio of lipid. The lipid stock consisted of a 10:1 mix by mass of 7.7 monoacylglycerol (generously provided by Martin Caffrey) with cholesterol (Sigma). Samples were reconstituted by the two syringe mixing method10 (link) and then dispensed into glass sandwich plates using a GryphonLCP robot (Art Robbins Instruments). In the case of the β2AR-adrenaline complex, 1 mM fresh adrenaline was mixed with receptor prior to reconstitution. Crystals were grown using 30 nL protein/lipid drops with 600 nL overlay solution, which consisted of 18 – 24 % PEG400, 100 mM MES pH 6.2 to pH 6.7, and 40 – 100 mM ammonium phosphate dibasic. Crystals grew in 1 – 3 days, and were harvested and frozen in liquid nitrogen for data collection.
Adults patients (≥ 18 years) with sepsis and complete fluid administration records were screened in the analysis. The following exclusion criteria were used: (1) patients who have not received any crystalloids administration; (2) patients who received albumin longer than 24 h after the initiation of crystalloids administration or preceded the crystalloids. For patients who had ICU admission more than once, only data of the first ICU admission of the first hospital stay were included.
Most recents protocols related to «Epinephrine»
Example 16
Direct analysis of chemicals in animal tissue using probes of the invention was performed as shown in
Lipid profiles were obtained for human prostate tissues (1 mm2×15 μm,
Example 6
A lidocaine preservative free intranasal formulation with combination of other drugs is prepared using the ingredients set forth in Table 4 for Examples 6-8.
The formulation is prepared as follows: Add citric acid monohydrate to purified water while stirring and mix till a clear solution is observed. Add lidocaine base or salt, combination drug and other optional excipients while stirring and mix for 30 minutes till a clear solution is formed. Filter the clear solution using sterile 0.2 micron pore size filter and fill the solution in a glass bottle aseptically and tightly crimp metered dose mechanical pump.
Nitric oxide decomposes rapidly to form stable metabolite nitrite/nitrate products. The nitrite level was measured and used as an index of nitric oxide (NO) production using the Griess reagent. A total of 0.5 ml of plasma was precipitated with 200 μl of 30% sulphosalicylic acid, vortexed for 30 min, and centrifuged at 3000 × g. Equal volumes of supernatant and Griess reagent containing 1% sulphanilamide in 5% phosphoric acid/0.1% naphthalene ethylenediamine dihydrochloride were added and incubated for 10 min in the dark, and the sample was measured at 543 nm. The nitrite levels were calculated using sodium nitrite as the standard [13 (link)].
The O2− concentration was measured after the reaction of nitro blue tetrazolium in Tris buffer with the plasma at 530 nm. Distilled water served as the blank [14 ].
The measurement of H2O2 is based on the oxidation of phenol red by H2O2 in a reaction catalysed by horseradish peroxidase (HRPO). Two hundred μl of plasma was precipitated with 800 ml of freshly prepared phenol red solution, followed by the addition of 10 μl of (1:20) HRPO (made ex tempore). Distilled water was used as the blank instead of the plasma sample. H2O2 was measured at 610 nm [15 (link)].
The degree of lipid peroxidation in the plasma samples was estimated by measuring TBARS using 1% thiobarbituric acid in 0.05 NaOH, incubated with the plasma at 100 °C for 15 min, and measured at 530 nm. Distilled water served as the blank [16 (link)].
The activity of the following antioxidants in the lysate was determined: reduced glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD). The level of reduced glutathione was determined based on GSH oxidation with 5,5-dithiobis-6,2-nitrobenzoic acid using a method by Beutler [17 ]. The CAT activity was determined according to Aebi [18 (link)]. The lysates were diluted with distilled water (1:7 v/v) and treated with chloroform-ethanol (0.6:1 v/v) to remove haemoglobin, and then 50 μl of CAT buffer, 100 μl of sample and 1 ml of 10 mM H2O2 were added to the samples. The detection was performed at 360 nm. SOD activity was determined by the epinephrine method of Beutler [19 (link)]. Lysate (100 μl) and 1 ml carbonate buffer were mixed, and then 100 μl of epinephrine was added. The detection was performed at 470 nm.
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More about "Epinephrine"
It plays a vital role in the body's stress response, triggering the 'fight-or-flight' reaction.
Epinephrine acts on multiple organ systems, including the cardiovascular, respiratory, and metabolic systems, to prepare the body for intense physical activity.
It increases heart rate, blood pressure, and glucose levels, while also dilating the airways.
Epinephrine is widely used in medical settings to treat anaphylactic shock, cardiac arrest, and other emergencies.
Researchers studying the effects and mechanisms of epinephrine can utilize PubCompare.ai's AI-driven platform to quickly locate the best protocols from literature, preprints, and patents, enhancing reproducibility and accuracy in their epinephrine studies.
Synonyms and related terms for epinephrine include adrenaline, norepinephrine, and catecholamines.
Abbreviations like EPI and NE are also commonly used.
Key subtopics in epinephrine research may include the biosynthesis and metabolism of epinephrine, its interactions with receptors (e.g., alpha and beta adrenergic receptors), and its effects on physiological processes like cardiovascular function, respiration, and energy metabolism.
Researchers may also study related compounds like bovine serum albumin (BSA), which is sometimes used in epinephrine assays, or hydrogen peroxide (H2O2), which can be used to induce oxidative stress in epinephrine-related experiments.
Additionally, techniques like GIF-Q260J and VIO300D may be employed to measure epinephrine levels, while trichloroacetic acid (TCA) and thiobarbituric acid (TBA) are used in assays to assess epinephrine-related parameters.
By leveraging PubCompare.ai's powerful tools, researchers can streamline their epinephrine studies, improve reproducibility, and gain deeper insights into this crucial stress hormone and its multifaceted physiological effects.