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Erastin
Erastin
Erastin is a small molecule that induces a type of programmed cell death known as ferroptosis.
It acts by inhibiting the cystine/glutamate antiporter system xc-, which leads to depletion of intracellular glutathione and subsequent accumulation of lipid peroxides.
Erastin has been widely used as a research tool to study the mechanisms and biological consequences of ferroptosis.
PubCompare.ai can help optimize your Erastin research by providing easy access to relevant protocols from literature, preprints, and patents, as well as data-driven comparisons to identify the best experimental approaches and products.
Enhance the reproducibility and accuracy of your Erastin studies today.
It acts by inhibiting the cystine/glutamate antiporter system xc-, which leads to depletion of intracellular glutathione and subsequent accumulation of lipid peroxides.
Erastin has been widely used as a research tool to study the mechanisms and biological consequences of ferroptosis.
PubCompare.ai can help optimize your Erastin research by providing easy access to relevant protocols from literature, preprints, and patents, as well as data-driven comparisons to identify the best experimental approaches and products.
Enhance the reproducibility and accuracy of your Erastin studies today.
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Alamar Blue
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Erastin was synthesized as described (Yagoda et al., 2007 (link)). Additional erastin and Sorafenib analogs were prepared as described in the Supplementary file 1 . Data also available as ‘Extended Materials and Methods’ from Dryad data Repository (Dixon et al., 2014 (link)). The synthesis of (1S, 3R)-RSL3 was described (Yang et al., 2014 (link)). Sorafenib, imatinib, erlotinib, lapatinib, nilotenib, dasatinib, sunitinib, and gefetinib were from SelleckChem (Houston, USA). Unless otherwise indicated, all other compounds were from Sigma-Aldrich (St. Louis, USA).
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Anabolism
Dasatinib
erastin
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Cells were seeded at 3 × 105 cells per well in 6-well plates. Next day, cells were treated with Erastin (10 μM) and/or hemin (5 μM), CORM (10 μM), biliverdin (10 μM) for 8 hours. After 8 hours, cells were incubated with 2 μM CellROX® Deep Red (cytosolic ROS) or 2 μM C11-BODIPY581/591 (lipid peroxidation) (Invitrogen, Life Technologies, Grand Island, NY) for 30 minutes at 37°C in the dark. After 30 minutes of loading, unincorporated dye was removed by washings with 2% FBS containing PBS. Samples were then centrifuged at 1000 rpm for 3 minutes and the pellets were resuspended in 500 μL of 2% FBS containing PBS. Measurements were performed on a FACSCalibur (Becton Dickinson, San Jose, CA) flow cytometer.
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Biliverdine
BODIPY581
Cells
Cytosol
erastin
Hemin
Lipid Peroxidation
Pellets, Drug
Human LCL cultures GM18870 (G/G, wild-type p53), GM18871 (A/A, homozygous S47), and GM18872 (A/G, heterozygous child from GM18870 and GM18871 parents) were identified for genotype at rs1800371 using the 1000 Genomes Web site and were obtained from the Coriell Institute. These were grown in RPMI supplemented with 15% fetal bovine serum (FBS) and 1% 100 IU/mL penicillin/100 µg/mL streptomycin (pen/strep). The genotype of these lines was confirmed by DNA sequencing. H1299 p53-null human non-small-cell lung carcinoma cells containing a tetracycline regulatory element (H1299 T-Reg) were provided by Steven McMahon (Thomas Jefferson University). The tetracycline-inducible p53 plasmid (Plenti4/TO/V5-DEST) was subjected to site-directed mutagenesis to generate the S47 variant. H1299 T-Reg wild-type or S47 p53 cells were maintained at 37°C in Dulbecco's modified Eagle's medium (DMEM; Cellgro), 1% pen/strep (Cellgro, 30-002-CI), and 10% tetracycline approved FBS (Clontech, 631106). MEFs were obtained from 12.5-d-old Hupki mice and were cultured at 37°C in DMEM with 1% pen/step and 10% FBS (Gemini, 100–106). Etoposide (Sigma, E1383), CDDP (Acros Organics, 193760010), and carboplatin (Sigma, C2538) were used at the indicated concentrations. Doxycycline (BD Biosciences, 631311) was used at a concentration of 100 ng/mL. RSL3 (Aobious, Inc., AO1514), erastin (Sigma Aldrich, 571203-78-6), and Fer-1 (Sigma Aldrich, SML0583) were used at the concentrations indicated.
Carboplatin
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Child
Cisplatin
Doxycycline
erastin
Etoposide
Fetal Bovine Serum
Genome
Genotype
Heterozygote
Homo sapiens
Homozygote
Mus
Mutagenesis, Site-Directed
Non-Small Cell Lung Carcinoma
Null Cell
Parent
Penicillins
Plasmids
Regulatory Sequences, Nucleic Acid
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Most recents protocols related to «Erastin»
Erastin and RSL3 compounds were discovered initially as inducers for the occurrence of ferroptosis. The mechanism of both compounds for inducing ferroptosis involves targeting GPX4. Erastin and RSL3 can both directly lead to the inactivation of GPX4. In the case of Erastin, it can also indirectly lead to the inactivation of GPX4 by inhibiting the conversion of cystine, an essential component of glutathione synthesis. Consequently, lipid peroxidation is catalyzed, ultimately resulting not only in ferroptosis but also in neurodegenerative processes (Conteh et al., 2019 (link)).
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To determine the role of VDAC2 on tardigrade tun formation, erastin inhibition was conducted using 3 replicates of 30 tardigrades, each tardigrade in an individual well of a 96-well plate. Tardigrades were incubated in a 100 nM working concentration of erastin (MedChemExpress, Monmouth Junction, NJ) in 0.1% DMSO overnight. After 20 h, tardigrades were observed under a dissecting light microscope and manually counted to determine the number of tuns formed.
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According to the results of the concentration gradient test, we treated U251 or U87MG cells with erastin or RAS-selective lethal 3 (RSL3) for 24 h.
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For the potential functions and signaling pathways of erastin-induced gene expression changes in PCa cells to be further explored, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome analyses were performed to explore and study the different overlapping DEGs of the LNCaP group (LNCaP_5_0_era and LNCaP) and the PC3 group (PC3_5_0_era and PC3).
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Following capillary isolation, the pellet was resuspended in a cold PBS buffer solution and plated into 2-well chamber slides. Capillaries could then be treated with various antagonists, agonists, or inhibitors. For dosing experiments, serial dilutions of erastin were prepared immediately before use in PBS buffer solution using 10.0 mM erastin stock solution made in DMSO. Solutions containing the fluorescent substrate specific for P-glycoprotein (NBD-CSA) and appropriate dosing of erastin were then added to respective chamber slides at volumes of 2.0 mL. Time course studies were staggered such that each slide could be viewed consecutively on a confocal microscope at 15 min/slide. For studies involving BCRP and MRP2, fluorescent substrates BODIPY® FL prazosin and sulforhodamine 101 free acid (Texas Red) were used for the transporters, respectively. Transport activity was measured as a function of steady-state luminal fluorescence. In the P-gp studies, background fluorescence was measured using the P-gp-specific inhibitor, PSC833 10 μM (valspodar), and subtracted from raw treatment values to obtain specific luminal fluorescence as a result of inhibition or activation of the transporter.
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Top products related to «Erastin»
Sourced in United States, China
Erastin is a chemical compound used as a research tool in laboratory settings. It functions as a small molecule inhibitor that induces ferroptosis, a form of regulated cell death. The core function of Erastin is to serve as a tool for studying cellular processes and potential therapeutic applications related to ferroptosis.
Sourced in United States, Germany, Morocco, China
Erastin is a chemical compound used as a laboratory research tool. It functions as a ferroptosis inducer, capable of triggering a specific form of regulated cell death. The core function of Erastin is to disrupt cellular processes related to iron metabolism and lipid peroxidation. Detailed information about intended use or applications is not provided.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
Sourced in United States, China
Erastin is a small molecule compound that functions as a potent and selective inhibitor of the system xc- cystine/glutamate antiporter. It is widely used in research applications to induce ferroptosis, a form of regulated cell death.
Sourced in United States, China
Ferrostatin-1 is a chemical compound used as a research tool in laboratory settings. It functions as a selective inhibitor of ferroptosis, a form of programmed cell death. The core purpose of Ferrostatin-1 is to serve as an experimental tool for investigating cellular processes and mechanisms related to ferroptosis.
Sourced in United States, Germany, United Kingdom, Italy, Japan
Ferrostatin-1 is a chemical compound used in research laboratories. It functions as a potent inhibitor of ferroptosis, a form of programmed cell death. Ferrostatin-1 is utilized in various experimental settings to study cellular mechanisms and pathways related to ferroptosis.
Sourced in United States, Germany, China, Italy, Japan
BODIPY 581/591 C11 is a fluorescent lipid probe used for the detection and quantification of lipid peroxidation in biological samples. It exhibits a shift in fluorescence emission from red to green upon oxidation, allowing for the monitoring of oxidative processes.
Sourced in United States, China, Germany, Switzerland, United Kingdom
Z-VAD-FMK is a broad-spectrum caspase inhibitor. It functions by irreversibly binding to the catalytic site of caspase enzymes, thereby blocking their activity.
Sourced in United States
Erastin is a small molecule that functions as an inhibitor of system xc-, a cystine/glutamate antiporter. It is used in research applications to induce a form of regulated cell death known as ferroptosis.
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
More about "Erastin"
Erastin is a powerful small molecule that induces a unique form of programmed cell death known as ferroptosis.
It works by inhibiting the cystine/glutamate antiporter system xc-, leading to depletion of intracellular glutathione and subsequent accumulation of lipid peroxides.
This mechanism of action makes Erastin a valuable research tool for studying the intricate pathways and biological consequences of ferroptosis.
Beyond Erastin, other key terms and compounds related to this field of research include fetal bovine serum (FBS), which is commonly used in cell culture media, Ferrostatin-1 as a ferroptosis inhibitor, BODIPY 581/591 C11 as a lipid peroxidation fluorescent probe, and Z-VAD-FMK as a pan-caspase inhibitor.
Penicillin and streptomycin are also frequently used antibiotics in cell culture experiments.
PubCompare.ai is an AI-powered platform that can help optimize your Erastin-related research by providing easy access to relevant protocols from literature, preprints, and patents.
The tool also offers data-driven comparisons to identify the best experimental approaches and products, enhancing the reproducibility and accuracy of your Erastin studies.
Leveraging these resources can be a game-changer in your ferroptosis research, leading to more reliable and impactful findings.
It works by inhibiting the cystine/glutamate antiporter system xc-, leading to depletion of intracellular glutathione and subsequent accumulation of lipid peroxides.
This mechanism of action makes Erastin a valuable research tool for studying the intricate pathways and biological consequences of ferroptosis.
Beyond Erastin, other key terms and compounds related to this field of research include fetal bovine serum (FBS), which is commonly used in cell culture media, Ferrostatin-1 as a ferroptosis inhibitor, BODIPY 581/591 C11 as a lipid peroxidation fluorescent probe, and Z-VAD-FMK as a pan-caspase inhibitor.
Penicillin and streptomycin are also frequently used antibiotics in cell culture experiments.
PubCompare.ai is an AI-powered platform that can help optimize your Erastin-related research by providing easy access to relevant protocols from literature, preprints, and patents.
The tool also offers data-driven comparisons to identify the best experimental approaches and products, enhancing the reproducibility and accuracy of your Erastin studies.
Leveraging these resources can be a game-changer in your ferroptosis research, leading to more reliable and impactful findings.