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Ethane sulfonate

Ethane sulfonate is a chemical compound used in various research applications, including the study of biological processes and the development of new pharmaceuticals.
This versatile molecule has been the subject of extensive investigation, with researchers exploring its potential applications in areas such as cell signaling, enzyme regulation, and drug delivery.
PubCompare.ai's AI-driven optimization platform can enhance the reproducibility and accuracy of your Ethane sulfonate research by helping you locate the best protocols from literature, pre-prints, and patents, using intelligent comparisons to identify the optimal approaches.
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Most cited protocols related to «Ethane sulfonate»

Genetic Screens Reveal Nonsense-Mediated Decay Factors

Cited 5 times

GAL4/UAS system [27 (link)] drivers used were btl-GAL4 [44 ], e22c-GAL4 [45 (link)], ppk-GAL4 [32 (link)], and da-GAL4 [24 (link)]. GFP and DsRed transgenes are referenced in Table 1. Df(Smg1)exe2B was generated by using FLP-mediated recombination between the FRTs in P{XP}C3G[d00589] and PBac{WH}CG3044[f02328] as described in Thibault et al. [46 (link)]. Marker mutations and balancer chromosomes are described at http://www.flybase.org. Flies were reared at 25 °C on cornmeal/dextrose medium.
The photoshop mutations were obtained by mutagenesis of an isogenized y w FRT^19A chromosome [22 (link)] with 25 mM ethane methyl sulfonate overnight [47 ] in a tracheal mutant screen (to be described elsewhere). The mutations used were on this chromosome unless otherwise noted. To generate homozygous mutant clones, 2- to 6-h-old embryos were collected at 25 °C from a cross of y w * FRT^19A/FM7 females to gal80 FRT^19A, hsFLP122/Y; btl-GAL4, UAS-GFP males. After a 45-min heat shock at 38 °C to induce FLP expression, embryos were returned to 25 °C to continue development. L3 larvae of genotype y w * FRT^19A/gal80 FRT^19A, hsFLP122; btl-GAL4, UAS-GFP/+ were identified by GFP mosaicism within the tracheal system and scored for the photoshop phenotype.
The original Smg1^32AP chromosome (designated 32AP†) carried lethal mutations not associated with the photoshop phenotype. Lethals were removed by crossing 32AP†/y w FRT^19A females to w/Y; btl-GAL4, UAS-GFP males and identifying L3 larvae with enhanced GFP throughout their tracheal systems. These Smg1^32AP/Y; btl-GAL4, UAS-GFP/+ larvae developed into viable adult males. We also found a viable wing morphology mutation on 32AP† that is allelic to wavy. Existing wavy alleles do not show a photoshop phenotype, and the wing phenotype is separable from the photoshop phenotype, so wavy does not seem to contribute to the photoshop phenotype.
The Upf2^29AA chromosome carries a linked lethal mutation. When recombined away from Upf2^29AA, the mutation had no effect on tracheal development or reporter expression. However, we have not obtained a recombinant containing Upf2^29AA without the extraneous mutation.
For complementation tests of Upf2, we used genomic rescue transgenes located on the autosomes to generate males of genotype y w Upf2^14J v g f FRT^19A/Y; P{w⁺, Upf2⁺}/+ and crossed these to y w * FRT^19A/FM7c females, where the asterisk indicates the tested mutation. Absence of Bar⁺, white-eyed female progeny indicated failure to complement. For complementation tests of Upf1, we used the Y-linked duplication Dp(1;Y)BSC1, y⁺, which covers the Upf1 locus. Males of genotype Upf1^13D/Dp(1;Y)BSC1, y⁺ were crossed to Upf1^26A/FM7c females, and the absence of female Bar⁺ progeny indicated a failure to complement.

Metzstein M.M, & Krasnow M.A. (2006). Functions of the Nonsense-Mediated mRNA Decay Pathway in Drosophila Development. PLoS Genetics, 2(12), e180.

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Publication 2006

Adult Adult Fanconi Syndrome Alleles Chromosomes Chromosomes, Human, Pair 22 Clone Cells Diptera Embryo ethane methanesulfonate Females Genetic Complementation Test Genome Genotype Glucose Heat-Shock Response Homozygote Larva Males Mosaicism Mutagenesis Mutation Phenotype Recombination, Genetic Trachea Transgenes

Genetic Mechanisms in Drosophila Development

Cited 4 times

Mutations are described at http://flybase.org. Wild type was yellow white or Histone-GFP. All experiments were performed at 25°C unless noted otherwise. cno^R2 was generated by ethane methyl sulfonate on an isogenic FRT82B line. cno^R2 was sequenced by PCR amplifying fragments of the cno coding sequence and sequencing them at the University of North Carolina at Chapel Hill Genome Analysis Facility. Cuticle preparations were made as described previously in Wieschaus and Nüsslein-Volhard (1986) . Unless noted otherwise, fly stocks were obtained from the Bloomington Stock Center. Sources of other stocks are provided in Table S1. cno germline clones were made by heat shocking 48–72-h-old hsFLP¹; FRT82Bcno^R2/FRT82Bovo^D1-18 larvae for 3 h at 37°C. arm^043A01 and ed^F72 germline clones were generated similarly.

Sawyer J.K., Harris N.J., Slep K.C., Gaul U, & Peifer M. (2009). The Drosophila afadin homologue Canoe regulates linkage of the actin cytoskeleton to adherens junctions during apical constriction. The Journal of Cell Biology, 186(1), 57-73.

Publication 2009

Clone Cells ethane methanesulfonate Genome Germ Line Histones Larva Mutation Open Reading Frames

Genetic Manipulation of Drosophila zydeco

Cited 4 times

Flies were cultured on standard medium at 22°C. zydeco (zyd) mutants were generated by ethane methyl sulfonate (EMS) mutagenesis and identified in a screen for temperature-sensitive (TS) behavioral phenotypes (Guan et al., 2005 (link)). zyd¹ was mapped to the X heterochromatic region containing CG2893 by recombination and complementation analysis with deficiency chromosomes. Mutation of CG2893 in three independently generated zyd alleles was identified by sequencing and comparison to control genomic sequence. We used the UAS/Gal4 system to drive transgenes in glia. UAS-zyd was constructed by subcloning the zydeco cDNA into pUAST and injected into w¹¹¹⁸ by Genetic Services. UAS-myrGCaMP5 was constructed by subcloning the first 90 aa of Src64b, containing a myristoylation target sequence, into pBI-UASc (creating pBI-UASc-myr). GCaMP5G cDNA (Addgene plasmid 31788) was cloned into pBI-UASc-myr and injected using ϕ-C-31 transformation.

Melom J.E, & Littleton J.T. (2013). Mutation of a NCKX Eliminates Glial Microdomain Calcium Oscillations and Enhances Seizure Susceptibility. The Journal of Neuroscience, 33(3), 1169-1178.

Publication 2013

Alleles Chromosomes Culture Media Diptera DNA, Complementary ethane methanesulfonate Genome Mutagenesis Mutation Neuroglia Phenotype Plasmids Recombination, Genetic Service, Genetic Transgenes

Genetic Manipulations of Synaptic Proteins

Cited 4 times

Control animals are isogenized (y w ey-FLP GMR-lacZ; FRT80B) unless otherwise indicated. 3L1¹, 3L1², and 3L1³ mutants (y w ey-FLP GMR-lacZ;3L1^x FRT80B/TM6B, Tb) were isolated from an ey-FLP ethane methyl sulfonate screen as described previously (Verstreken et al., 2003 (link)) with modifications. cspx¹ mutants and UAS-ssp (w;; P{w⁺UAS-csp-11c/s}, cspu¹) flies were provided by K. Zinsmaier (University of Arizona, Tucson, AZ). P-element stocks and deficiencies were obtained from the Bloomington Drosophila Stock Center (Bellen et al., 2004 (link); Parks et al., 2004 (link)), and 3L1 mapping was performed as described previously (Zhai et al., 2003 (link)).
We made a genomic rescue construct by PCR amplifying the 6.5-kb hip14 region from bacterial artificial chromosome clone AC093499. The fragment was cloned into the SalI restriction site of pP{CaSpeR-4} and sequenced. A cDNA construct was made by PCR amplifying hip14 from expressed sequence tag clone LD10758. The fragment was cloned into NotI and XbaI sites of pP{UAST} and sequenced.
To generate genomic GFP-tagged constructs, we first integrated an NheI site just before the ATG start codon (NtermGFP-HIP14) or after the hip14 stop codon (CtermGFP-HIP14) by site-directed mutagenesis (Stratagene). PCR-amplified EGFP sequence was cloned into the NheI site.
We generated chimeric n-SybTMD-CSP constructs by PCR amplifying 111-bp N-terminal and 234-bp C-terminal n-syb sequences from pP{UAST}-syb-GFP and the full-length CSP2 from pP{UAST}-csp2 (provided by K. Zinsmaier). In the next round of PCR, we fused them to generate N-terminal-Syb-csp2-C-terminal-Syb chimeric (SybTMD-csp2). After sequencing, SybTMD-csp2 was cloned into pP{UAST} at NotI and XbaI.
P{w⁺UAS-SybTMD-csp2} and P{w⁺UAS-csp2} were expressed using elav-GAL4. For analyses of CSP localization and physiology of third instar larvae, we generated elav-GAL4/+; P{w⁺UAS-SybTMD-csp2} hip14² FRT80B/Df(3L)brm11 and elav-GAL4/+; hip14² FRT80B P{w⁺UAS-csp2}/Df(3L)brm11.

Ohyama T., Verstreken P., Ly C.V., Rosenmund T., Rajan A., Tien A.C., Haueter C., Schulze K.L, & Bellen H.J. (2007). Huntingtin-interacting protein 14, a palmitoyl transferase required for exocytosis and targeting of CSP to synaptic vesicles. The Journal of Cell Biology, 179(7), 1481-1496.

Publication 2007

Animals Bacterial Artificial Chromosomes Chimera Codon, Initiator Codon, Terminator Diptera DNA, Complementary Drosophila ethane methanesulfonate Expressed Sequence Tags Genome LacZ Genes Larva Mutagenesis, Site-Directed Phosphorus physiology

CRAC Channel Activation and Regulation

Cited 2 times

CRAC channels were activated by passive Ca²⁺ store depletion with the Ca²⁺ chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA) included in the pipette solution. The standard internal solution was composed of 128 mM Cs–aspartate, 10 mM Cs–Hepes, 12 mM BAPTA, and 0.9 mM CaCl₂. In some experiments, either 3.2 mM Mg²⁺ or 30 μM IP₃ (Sigma-Aldrich) was added to standard solution. To detect single channel CRAC currents, the concentration of external divalent cations was lowered below 1 μM to enable Na⁺ to permeate and serve as the charge carrier. The standard external solution contained 145 mM Na-methane sulfonate, 5 mM NaCl, 10 mM N-hydroxyethyl-ethylenediamine-triacetic acid (HEDTA), 10 mM Hepes, and 10 mM d-glucose. For some experiments, Na⁺ was replaced with N-methyl-d-glucamine (NMDG⁺). Ca²⁺ currents were detected in Ca²⁺-containing solution: 155 mM Na-methane sulfonate, 5 mM NaCl, 2 mM Ca-methane sulfonate, 10 mM Hepes, and 10 mM d-glucose. Aliquots from Ca²⁺-saturated HEDTA solution were added to standard solution to yield the desired concentration of extracellular free Ca²⁺ (1, 10, and 50 μM). The pH of all solutions was 7.2, and osmolarity was 300–305 mOsm. The HEDTA stock solution saturated with Ca²⁺ was prepared using a pH-metric method (Neher 1988). Extracellular solutions and drugs were applied using a gravity-driven perfusion system with output tip diameter of ∼50 μm placed ∼50 μm from the cell. Five barrels were inserted close to the output tip, and solution exchange controlled manually by valves. A complete local solution exchange was achieved within 2 s. For [Ca²⁺]_i imaging, cells were bathed in normal Ringer solution consisting of 155 mM NaCl, 4.5 mM KCl, 1 mM MgCl₂, 2 mM CaCl₂, 10 mM d-glucose, 5 mM Hepes, pH 7.4. Ca²⁺-free Ringer solution contained 1 mM EGTA in place of CaCl₂ and a total of 3 mM MgCl₂. Ca²⁺ concentration in normal Ringer solution was lowered to 300 μM to reduce the rate of capacitative Ca²⁺ influx in some measurements. In K⁺ Ringer, KCl replaced NaCl.

Fomina A.F., Fanger C.M., Kozak J.A, & Cahalan M.D. (2000). Single Channel Properties and Regulated Expression of Ca2+ Release-Activated Ca2+ (Crac) Channels in Human T Cells. The Journal of Cell Biology, 150(6), 1435-1444.

Publication 2000

1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid Acids Aspartate Calcium Release Activated Calcium Channels Cations, Divalent Cells Chelating Agents Egtazic Acid Ethane Ethylenediamines Glucose Gravity HEPES Magnesium Chloride methanesulfonate Osmolarity Perfusion Pharmaceutical Preparations Ringer's Solution Sodium Chloride

Most recents protocols related to «Ethane sulfonate»

Synthesis of H2TPDC-MIMS Ligand

H₂TPDC-MIMS was obtained by de-esterfication of the precursor 2,2’-((4,4”-bis(methoxycarbonyl)-[1,1’:4’,1”-terphenyl]-2’,5’-diyl)bis(1H-imidazole-3-ium-1,3-diyl))bis(ethane-1-sulfonate) (P1) received from lab. To the solution of P₁ (1.502 g, 2 mmol) dissolved in mixed MeOH/H₂O (v: v = 3:1) (90 ml), LiOH (95.92 mg, 4 mmol) was added with stirring. Then the clear solution was refluxed for 12 h. After cooling to room temperature, the mixture was acidified to pH = 1 with HCl (conc, 37% w/w) to yield a white precipitate, which was filtered, washed with water, and further dried under vacuum to obtain ligand H₂TPDC-MIMS (1.372 g, 95%). ¹H NMR (600 MHz, DMSO-d₆): δ 1.97 (4H, m), 2.38 (4H, t, J = 7.04 Hz), 4.18 (4H, t, J = 8.07 Hz), 5.45 (4H, s), 6.77 (2H, s), 6.86 (2H, s), 7.03 (2H, s), 7.32 (2H, s), 7.47 (4H, d, J = 5.62 Hz), 8.01 (4H, d, J = 5.38 Hz), 13.09 (2H, s) (Supplementary Fig. 34).

Xue W.L., Li G.Q., Chen H., Han Y.C., Feng L., Wang L., Gu X.L., Hu S.Y., Deng Y.H., Tan L., Dove M.T., Li W., Zhang J., Dong H., Chen Z., Deng W.H., Xu G., Wang G, & Wan C.Q. (2024). Melt-quenched glass formation of a family of metal-carboxylate frameworks. Nature Communications, 15, 2040.

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Publication 2024

Synthesis of Polyurethane Hydrogel Nanocomposites

The raw materials used in this study include poly(tetramethylene glycol) , hydroxyl terminated polybutadiene , neopentyl glycol (NPG), isophorone diisocyanate (IPDI, used as received), 2,2-bis(hydroxymethyl)propionic acid (DMPA), N-methylpyrrolidone (NMP), sodium 2-[(2-amino ethyl)amino]ethane sulfonate (AAS salt), ethylenediamine (EDA), trimethylamine (TEA), neodecanoic acid bismuth(3⁺) salt. All these raw materials were analytical reagents and purchased from Macklin Chemical Reagent Co. Ltd. (Shanghai, China).

Liu J., Yang D., Li S., Bai C., Tu C., Zhu F., Xin W., Li G, & Luo Y. (2023). Synthesis and characterization of hydroxyl-terminated polybutadiene modified low temperature adaptive self-matting waterborne polyurethane. RSC Advances, 13(10), 7020-7029.

Publication 2023

1-methyl-2-pyrrolidinone Bismuth ethane sulfonate Ethylenediamines Glycols Hydroxyl Radical isophorone diisocyanate N,N-dimethyl-4-anisidine neodecanoic acid Poly A polybutadiene propionic acid Sodium Sodium Chloride trimethylamine

Polysaccharide Hydrogel Synthesis

Cyclomaltoheptaose known as Beta-cyclodextrin (CID-444041), Cysteine HCl (CID-60960), Ellman’s reagent (3-Carboxy-4-nitro phenyl disulfide) (CID-6254), MES hydrate (Sodium 2-morpholino ethane sulfonate) (CID-23673676), cysteamine (CID-6058), sodium periodate (CID-23667635), sodium cyanoborohydride (CID-20587905), dimethyl sulfoxide (DMSO), ethylene glycol, minoxidil (MXD), distilled water, chitosan, and sodium phosphate monobasic dihydrate (purity ≥ 99%) were obtained from Glentham Life Sciences, UK. Analytical grade chemicals and reagents were purchased from International chemical suppliers. Pretreated regenerated cellulose dialysis tubing of 1000–2000 Da was purchased from Spectrum, USA.

Akhtar A., Waqas M.K., Mahmood A., Tanvir S., Hussain T., Kazi M., Ijaz M, & Asim M.H. (2023). Development and Characterization of Thiolated Cyclodextrin-Based Nanoparticles for Topical Delivery of Minoxidil. Pharmaceutics, 15(12), 2716.

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Publication 2023

Synthesis of Sulfonated Ether Compound

Not available on PMC !

Example 5

[Figure (not displayed)]

A stirred mixture of 1-bromo-2-[2-(2-methoxyethoxy)ethoxy]ethane (2.50 g, 11.0 mmol) and sodium sulfite [Na₂SO₃](1.39 g, 11.0 mmol) in H₂O (13.4 mL) was heated at reflux for 18 h. The mixture was concentrated under reduced pressure, then taken up in ethanol (20 mL) and concentrated to dryness. A 1:1 mixture of TBME/hexanes (20 mL) was added, and again the mixture was concentrated to dryness. This final step was repeated to give sodium 2-[2-(2-methoxyethoxy)ethoxy]ethane-1-sulfonate as a solid (as a mixture with sodium bromide) which was used without analysis or purification. POCl₃(6.8 mL, 74.1 mmol) was added, and the mixture heated at 70° C. for 4 h, then stirred at rt overnight. The mixture was added carefully to ice-H₂O (50 mL), then the product extracted with EtOAc (3×50 mL). The combined organic extracts were dried (Na₂SO₄) and concentrated under reduced pressure to give 2-[2-(2-methoxyethoxy)ethoxy]ethane-1-sulfonyl chloride) (1.90 g, 70%) as an oil. ¹H NMR (400 MHz, CDCl₃) δ 4.14-4.04 (m, 2H), 3.99 (t, J=6.0 Hz, 2H), 3.78-3.69 (m, 2H), 3.69-3.61 (m, 4H), 3.55 (dd, J=5.7, 3.4 Hz, 2H), 3.38 (s, 3H).

US11603377B2. MTORC1 modulators and uses thereof (2023-03-14). Aeovian Pharmaceuticals, Inc. [US]. Inventors: John Kincaid [US].

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Patent 2023

1H NMR Anabolism Ethane ethane sulfonate Ethanol Hexanes Pressure Sodium sodium bromide sodium sulfite sulfonyl chloride

Transient Expression Assay in N. benthamiana

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Publication 2023

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What is Ethane sulfonate and how is it used in research?

Ethane sulfonate is a versatile chemical compound that has been extensively studied for its potential applications in various areas of research. It is commonly used to explore biological processes, such as cell signaling and enzyme regulation, as well as in the development of new pharmaceuticals. This molecule has proven to be a valuable tool for researchers investigating a wide range of topics, from fundamental biological mechanisms to the advancement of therapeutic interventions.

What are some common challenges researchers face when working with Ethane sulfonate?

One of the key challenges researchers face when working with Ethane sulfonate is ensuring the reproducibility and accuracy of their experiments. The selection of the optimal protocol from the available literature, pre-prints, and patents can be a time-consuming and daunting task, especially when dealing with a large volume of information. Researchers may struggle to identify the most effective approaches that align with their specific research goals and experimental setups.

How can PubCompare.ai help researchers optimize their use of Ethane sulfonate?

PubCompare.ai's AI-driven optimization platform can greatly assist researchers in their Ethane sulfonate studies. The platform allows users to screen protocol literature more efficiently, leveraging AI to pinpoint critical insights. By comparing the effectiveness of different protocols related to Ethane sulfonate, PubCompare.ai can help researchers identify the most optimal approaches for their specific research goals, enhancing the reproducibility and accuracy of their experiments. The platform's intelligent analysis can highlight key differences in protocol effectiveness, enabling researchers to choose the best option for their work.

Are there different variations or types of Ethane sulfonate that researchers should be aware of?

Yes, there are various derivatives and analogs of Ethane sulfonate that researchers may encounter in the literature. These different forms of the molecule can have slightly varying properties, such as solubility, stability, or specific applications. Understanding the unique characteristics of these Ehane sulfonate variations can be important for selecting the most appropriate form for a particular research project or experimental setup. PubCompare.ai's platform can assist in identifying and comparing the different Ethane sulfonate variants to help researchers make informed decisions.

What are some specific applications of Ethane sulfonate in research?

Ethane sulfonatee has been utilized in a wide range of research applications. It has been studied for its role in cell signaling pathways, where it can modulate the activity of enzymes and other biomolecules. Researchers have also investigated the use of Ethane sulfontate in drug delivery systems, exploring its potential to enhance the bioavailability and efficacy of pharmaceutical compounds. Additionally, this versatile molecule has been employed in the study of metabolic processes, protein structure and function, and the development of new therapeutic strategies. The diverse applications of Ethane sulfontate highlight its importance in advancing our understanding of biological systems and driving innovation in the field of medical research.

More about "Ethane sulfonate"

Ethane sulfonate, a versatile chemical compound, has garnered significant attention in the research community due to its diverse applications.
This sulfur-containing molecule has been extensively studied for its potential roles in biological processes, drug development, and various other research areas.
Synonyms and related terms for ethane sulfonate include ethanesulfonate, ethyl sulfonate, and ethyl sulfonic acid.
These terms are often used interchangeably, reflecting the compound's chemical structure and functional groups.
Researchers have explored the use of ethane sulfonate in cell signaling, enzyme regulation, and drug delivery systems.
Its ability to interact with biological systems makes it a valuable tool for understanding cellular mechanisms and developing new therapeutic approaches.
Techniques such as the Agilent 2100 Bioanalyzer, [ 45 Ca]CaCl 2, and DMSO have been utilized in conjunction with ethane sulfonate to study its effects on biological samples and optimize experimental conditions.
Additionally, compounds like Bradykinin, Diazoxide, and Nifedipine have been investigated for their potential synergistic or antagonistic interactions with ethane sulfonate.
The optimization of ethane sulfonate research can be enhanced through the use of platforms like PubCompare.ai, which leverages AI-driven algorithms to identify the best protocols from literature, preprints, and patents.
This approach can improve the reproducibility and accuracy of studies, leading to more reliable and insightful findings.
By incorporating these insights and related terms, researchers can gain a deeper understanding of the versatility and applications of ethane sulfonate, ultimately advancing scientific knowledge and driving innovation in fields such as cell biology, pharmacology, and drug discovery.