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Ethanolamine

Ethanolamine is a primary amine and alcohol with the chemical formula H2NCH2CH2OH.
It is an important organic compound used in the production of surfactants, emulsifiers, and other industrial chemicals.
Ethanolamine has a variety of applications, including as a corrosion inhibitor, pH adjuster, and chemical intermediate.
Researcheres can use PubCompare.ai's AI-driven platform to enhance reproducibility and accuracy when optimizing Ethanolamine-related processes and products.
The platform helps locate the best protocols from literature, preprints, and patents, and provides data-driven insights to improve Ethanolamine research.

Most cited protocols related to «Ethanolamine»

184A1, BT20, BT474, BT483, BT549, Hs578T, hTERT-HME1, MCF7, MCF10A, MDA-MB134, MDA-MB157, MDA-MB175, MDA-MB231, MDA-MB361, MDA-MB436, MDA-MB453, MDA-MB468, SKBR3, T47D, UACC812, UACC893, ZR75-1 and ZR75-30 were obtained from ATCC (Manassas, VA, USA). EFM19 and EFM192A were obtained from DSMZ (Braunschweig, Germany). HCC38, HCC70, HCC202, HCC712, HCC1007, HCC1143, HCC1395, HCC1419, HCC1428, HCC1500, HCC1569, HCC1599, HCC1806, HCC1937, HCC1954, HCC2157, HCC2185, HCC2218, HCC2688 and HCC3153 were obtained from the cell repository of the Hamon Center for Therapeutic Oncology Research, UT Southwestern Medical Center (many are now available from ATCC). CAL51 was a kind gift from J. Gioanni from the Centre Antoine-Lacassagne, Nice, France. SUM44PE, SUM52PE, SUM102PT, SUM149PT and SUM190PT were kind gifts from Dr. Stephen P. Ethier (now available from Asterand, Detroit, MI). MCF10A was grown in MEGM media (Cambrex, East Rutherford, NJ). SUM52PE and SUM149PT were grown in Ham's F12 media with 5% FBS, supplemented with 5 µg/ml insulin and 1 µg/ml hydrocortisone. SUM44PE, SUM102PT and SUM190PT were grown in Ham's F12 with 0.1% BSA, supplemented with 5 µg/ml insulin, 1 µg/ml of hydrocortisone, 5 mM ethanolamine, 10 mM HEPES, 5 µg/ml transferrin, 10 nM of Triiodo Thyronin (T3) and 50 nM sodium selenite (10 ng/ml EGF was also included for SUM102PT). All other cell lines were grown in RPMI-1640 with 10% FBS and 1% Pen/Strep. Clinicopathological characteristics of cell lines are summarized in Table 1. A subset of cell lines (focused on the HCC series) was subjected to a more detailed molecular pathological characterization of ESR1, PGR, ERBB2, EGFR and BRCA1, as summarized in Table 2.
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Publication 2009
BRCA1 protein, human Cell Lines Cells EGFR protein, human ERBB2 protein, human Ethanolamine Gifts HEPES Hydrocortisone Insulin MCF-7 Cells Neoplasms Selenite, Sodium Streptococcal Infections Transferrin
We perform surface plasmon resonance experiments with a Biacore 3000 instrument. The immobilization involves activation of carboxymethyl groups on a dextran-coated chip by reaction with N-hydroxysuccinimide, followed by covalent bonding of the ligands to the chip surface via amide linkages and blockage of excess activated carboxyls with ethanolamine (6 (link)). Reference surfaces are prepared in the same manner, except that all carboxyls are blocked and no ligand is added. During analysis, each cell with an immobilized fusion polypeptide is paired with an adjacent cell on the chip, the latter serving as a reference. The final concentration of bound ligand, expressed in response units (RU), is calculated by subtracting the reference RU from the ligand RU. HBS-N, HBS-P, HBS-EP, or PBST buffer may be used as both running and analyte-binding buffer. Purified fusion polypeptide or protein (analyte), typically at 100 nM, is allowed to flow over the immobilized-ligand surface and the binding response of analyte to ligand is recorded. The chip surface is regenerated by removal of analyte with a regeneration buffer. The maximum RU with each analyte indicates the level of interaction, and reflects comparative binding affinity.
Publication 2009
Amides Buffers Cells Cells, Immobilized Dextran DNA Chips Ethanolamine Ligands Polypeptides Proteins Regeneration Surface Plasmon Resonance
Polyclonal anti-Slimb antibody was bound to equilibrated Protein A–coupled Sepharose (Sigma-Aldrich) by gently rocking overnight at 4°C in 0.2 M sodium borate. For GFP immunoprecipitations, GFP-binding protein (GBP; Rothbauer et al., 2008 (link)) was fused to the Fc domain of human IgG (pIg-Tail; R&D Systems), tagged with His6 in pET28a (EMD Millipore), expressed in E. coli, and purified on Talon resin (Takara Bio Inc.) according to manufacturer’s instructions. GBP was bound to Protein A–coupled Sepharose, cross-linked to the resin using dimethyl pimelimidate, and rocked for 1 h at 22°C; the coupling reaction was then quenched in 0.2 M ethanolamine, pH 8.0, and rocked for 2 h at 22°C. Antibody or GBP-coated beads were washed three times with 1.5 ml of cell lysis buffer (CLB; 100 mM Tris, pH 7.2, 125 mM NaCl, 1 mM DTT, 0.1% Triton X-100, and 0.1 mM PMSF). Transfected S2 cells were induced to express recombinant Cap-H2 with 1–2 mM CuSO4. After 24 h, transfected cells were lysed in CLB, clarified by centrifugation, and then diluted to 2–5 mg/ml in CLB. Antibody-coated beads were mixed with lysate for 40 min at 4°C, washed three times with CLB, and then boiled in Laemmli sample buffer. Lambda phosphatase (New England Biolabs, Inc.) treatments were performed for 1 h at 37°C. In vivo ubiquitination assays were performed by coexpressing Plk4-GFP (Rogers et al., 2009 (link)) or Cap-H2-GFP constructs with 3×FLAG–tagged Drosophila ubiquitin (CG32744), driven under the inducible metallothionein promoter pMT vector, immunoprecipitated using anti-GFP JL8 antibody, and analyzed by anti-FLAG immunoblotting.
Publication 2013
Antibodies, Anti-Idiotypic Binding Proteins Biological Assay Buffers Cells Centrifugation Claw Cloning Vectors dimethyl pimelimidate Drosophila Escherichia coli Ethanolamine Homo sapiens Immunoglobulins Immunoprecipitation Laemmli buffer Metallothionein Phosphoric Monoester Hydrolases Resins, Plant sodium borate Sodium Chloride Staphylococcal protein A-sepharose Tail TRAF3 protein, human Triton X-100 Tromethamine Ubiquitin Ubiquitination
Data were analyzed as described previously28 . Following a 1-Hz apodization, spectra were fitted with LCModel software19 (link), using calculated spectra of 20 metabolites as basis functions. The basis set included spectra of 2HG, NAA, GABA, glutamate, glycine, creatine, myo-inositol, glutamine, lactate, alanine, acetate, aspartate, ethanolamine, glutathione, phosphorylethanolamine, scyllo-inositol, taurine, N-acetylaspartylglutamate, glucose, and choline. The metabolite concentrations were estimated with respect to the short echo time water signal. Assuming an equal composition of gray and white matter in tumors, we used a water concentration value of 42.3 M, calculated from the literature values23 (link) for the water concentrations in gray and white matter. Relaxation effects on metabolite signals were corrected using published metabolite T2 and T1; T2 = 150, 230 and 280 for Cr, Cho and NAA, and 180 ms for other metabolites, respectively, and T1 = 1.2 for 2HG, glutamate, glutamine and myo-inositol, and 1.5 for other metabolites20 (link)–22 .
Publication 2011
Acetate Alanine Aspartate Choline Creatine ECHO protocol Ethanolamine gamma Aminobutyric Acid Glucose Glutamate Glutamine Glutathione Glycine Inositol Lactates MS 180 N-acetyl-aspartyl-glutamate Neoplasms phosphoethanolamine scyllitol Taurine White Matter
Biosensor experiments were conducted in a Biacore 3000 surface plasmon resonance (SPR) instrument equipped with microfluidic sample delivery (GE Healthcare, Piscataway NJ). Customarily, the binding signal is measured in units termed “RU”, for ‘resonance units’, approximately equivalent to a refractive index change near the surface of 10−6. Sensor chips CM3 with ‘short’ carboxy-methyl dextran matrix were used. Standard amine coupling with N-hydroxysuccinimide and (N-ethyl-N-(3-dimethylaminopropyl)carbodiimide, was used, and unreacted sites were quenched with ethanolamine HCl 16 (link), 31 . Hepes buffer saline with 3 mM EDTA and 0.005 % P20 was used as a running buffer. A number of different pairs of interacting molecules were studied, as indicated in the Results and Figure legends. Association and dissociation kinetics were recorded at a range of analyte concentrations, adjusting the contact time to achieve close to steady-state binding, and extending the dissociation time to allow monitoring significant curvature in the dissociation trace, where possible. Dependent on the interacting pairs of molecules, surface regeneration was achieved with a pulse of high salt solution, detergents, or by waiting for natural dissociation, respectively.
Publication 2008
Amines Biosensors Buffers Carbodiimides carboxymethyl dextran Detergents DNA Chips Edetic Acid Ethanolamine HEPES Kinetics Obstetric Delivery Regeneration Saline Solution Sodium Chloride Surface Plasmon Resonance Vibration

Most recents protocols related to «Ethanolamine»

EXAMPLE 1

A mixer, equipped with an electric mixer that has three prop-style mixing blades in series on a central shaft is used to produce a composition in accordance with the present disclosure. The tank itself is a stainless-steel cone-bottom tank with a 33 degree slope with a set of four baffles to allow for turbulent laminar flow.

36% w/w of dimethyl sulfoxide and 15% w/w of styrene-maleic anhydride copolymer are added to the tank, heated to 160° F., and mixed for one hour or until dissolved. 17% dicyandiamide is then added, and mixing continued for another hour or until dissolved. 15% monoethanolamine (MEA) is added with stirring and the resulting solution is allowed to cool to 100° F. Once cooled, 17% N-(N-butyl) Thiophosphoric Triamide (NBPT) is added with mixing for 45 minutes or until dissolved. The resulting solution is passed through a 5 micron filter, and samples are taken from both the top and the bottom of the reactor for testing. The resulting solution is reddish-orange and has a sulfur-like odor.

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Patent 2024
dicyandiamido Electricity Ethanolamine Odors Retinal Cone Stainless Steel Styromal Sulfoxide, Dimethyl Sulfur
Not available on PMC !

Example 8

[Figure (not displayed)]

To a solution of t-TUCB (0.31 g, 0.80 mmol) in 6 mL THF, benzotriazol-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate (PyBOP) (0.24 g, 0.65 mmol), ethanolamine (0.37 g, 6.0 mmol), and triethylamine (0.62 g, 6.1 mmol) were added. The mixture was stirred overnight at room temperature. Reaction mixture was diluted with EtOAc and washed with saturated NaHCO3 three times. After drying the organic layer with MgSO4, the solvent was evaporated and the crude product was used without further purification. MP=186.9-194.5° C. (189.5° C.) 1H NMR (400 MHz): 8.47 (s, 1H), 8.22 (t, 1H, J=5.6 Hz), 7.75 (d, 2H, J=8.8 Hz), 7.42 (d, 2H, J=9.2 Hz), 7.17 (d, 2H, J=8.8 Hz), 6.94 (d, 2H, J=8.8 Hz), 6.15 (d, 1H, J=7.6 Hz), 4.67 (t, 1H, J=5.6 Hz), 4.38 (m, 1H), 3.25 (9, 2H, J=6.4 Hz) 3.05-2.93 (m, 3H), 2.04-1.96 (m, 2H), 1.92-1.84 (m, 2H), 1.44 (9, 2H, J=12 Hz), 1.32 (9, 2H, J=12 Hz).

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Patent 2024
1H NMR benzamide Bicarbonate, Sodium Ethanolamine Solvents Sulfate, Magnesium triethylamine
Not available on PMC !

EXAMPLE 3

A mixer, equipped with an electric mixer that has three prop-style mixing blades in series on a central shaft is used to produce a composition in accordance with the present disclosure. The tank itself is a stainless-steel cone-bottom tank with a 33 degree slope with a set of four baffles to allow for turbulent laminar flow.

30.99% w/w of dimethyl sulfoxide and 20% w/w of styrene-maleic anhydride copolymer are added to the tank, heated to 160° F., and mixed for one hour or until dissolved. 0.01% FD&C Blue #1, 17% dicyandiamide, and 15% monoethanolamine (MEA) are then added, and mixing continued for another hour or until dissolved. The resulting solution is allowed to cool to 100° F., and then 17% N-(N-butyl) Thiophosphoric Triamide (NBPT) is added with mixing for 45 minutes or until dissolved. The resulting solution is passed through a 5 micron filter, and samples are taken from both the top and the bottom of the reactor for testing. The resulting solution is blue and has a sulfur-like odor.

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Patent 2024
brilliant blue FCF dicyandiamido Electricity Ethanolamine Odors Retinal Cone Stainless Steel Styromal Sulfoxide, Dimethyl Sulfur
Not available on PMC !

EXAMPLE 9

Affinity constants (Kd) of individual purified Nanobody clones were determined by surface plasmon resonance on a Biacore 3000 instrument. In brief, HuCD80-HuIgG1 or HuCD86-HuIgG1 were amine-coupled to a CM5 sensor chip at densities of 3000-4000 RU. Remaining reactive groups were inactivated using ethanolamine. Nanobody binding was assessed at 1 and 0.1 microM. Each sample was injected for 4 min at a flow rate of 45 μl/min to allow for binding to chip-bound antigen. Next, binding buffer without Nanobody was sent over the chip at the same flow rate to allow for dissociation of bound Nanobody. After 2 min, remaining bound analyte was removed by injecting regeneration solution (50 mM NaOH or Glycine/HCl pH 1.5). Binding curves obtained at different concentrations of Nanobody were used to calculate Kd values.

Kd values of selected Nanobody clones are shown in Table C-7.

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Patent 2024
Amines Antigens Buffers Clone Cells DNA Chips Ethanolamine Glycine Regeneration Surface Plasmon Resonance VHH Immunoglobulin Fragments
Not available on PMC !

EXAMPLE 2

A mixer, equipped with an electric mixer that has three prop-style mixing blades in series on a central shaft is used to produce a composition in accordance with the present disclosure. The tank itself is a stainless-steel cone-bottom tank with a 33 degree slope with a set of four baffles to allow for turbulent laminar flow.

40.99% w/w of dimethyl sulfoxide and 10% w/w of styrene-maleic anhydride copolymer are added to the tank, heated to 160° F., and mixed for one hour or until dissolved. 0.01% FD&C Blue #1, 17% dicyandiamide, and 15% monoethanolamine (MEA) are then added, and mixing continued for another hour or until dissolved. The resulting solution is allowed to cool to 100° F. and then, 17% N-(N-butyl) Thiophosphoric Triamide (NBPT) is added with mixing for 45 minutes or until dissolved. The resulting solution is passed through a 5 micron filter, and samples are taken from both the top and the bottom of the reactor for testing. The resulting solution is blue and has a sulfur-like odor.

Full text: Click here
Patent 2024
brilliant blue FCF dicyandiamido Electricity Ethanolamine Odors Retinal Cone Stainless Steel Styromal Sulfoxide, Dimethyl Sulfur

Top products related to «Ethanolamine»

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Ethanolamine is a chemical compound used in various laboratory applications. It serves as a key component in the production and analysis of organic compounds. Ethanolamine is a colorless, viscous liquid with a characteristic odor. Its primary function is to act as a chemical building block and buffer solution in various experimental and analytical procedures.
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The Biacore T200 is a label-free, real-time interaction analysis system designed for studying molecular interactions. It provides quantitative data on binding kinetics, affinity, and specificity between molecules. The system utilizes surface plasmon resonance (SPR) technology to detect and measure these interactions without the need for labeling.
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The CM5 sensor chip is a core component of the GE Healthcare's label-free detection platform. It is designed to measure biomolecular interactions in real-time without the need for labeling. The CM5 chip surface is made of a carboxymethylated dextran matrix that allows for the immobilization of a wide range of biomolecules, enabling the study of various types of interactions.
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The Biacore 3000 is a label-free, real-time biosensor system designed for the analysis of biomolecular interactions. The system utilizes surface plasmon resonance (SPR) technology to monitor interactions between immobilized molecules and molecules in solution, providing quantitative kinetic and affinity data.
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More about "Ethanolamine"

Ethanolamine, also known as 2-aminoethanol or monoethanolamine (MEA), is a versatile organic compound with a wide range of applications.
As a primary amine and alcohol, it has the chemical formula H2NCH2CH2OH.
Ethanolamine is an important industrial chemical used in the production of surfactants, emulsifiers, and other essential products.
Researchers can leverage the power of PubCompare.ai's AI-driven platform to enhance the reproducibility and accuracy of their Ethanolamine-related processes and product development.
The platform helps researchers locate the best protocols from literature, preprints, and patents, providing data-driven insights to improve their Ethanolamine research.
Ethanolamine has a variety of applications, including its use as a corrosion inhibitor, pH adjuster, and chemical intermediate.
It is commonly employed in the formulation of personal care products, metalworking fluids, and industrial cleaners.
Ethanolamine's versatility extends to its role as a building block for the synthesis of various amines, esters, and other derivatives.
In addition to its industrial uses, Ethanolamine has also been studied for its potential in biomedical applications.
Researchers have explored the use of Ethanolamine-based compounds in areas such as drug delivery, tissue engineering, and biocatalysis.
The ability to optimize Ethanolamine-related processes and products is crucial for advancing these emerging fields.
PubCompare.ai's AI-driven platform can help researchers streamline their Ethanolamine research by identifying the most effective protocols and methods from a vast array of literature, preprints, and patents.
By leveraging the platform's data-driven insights, researchers can improve the reproducibility and accuracy of their work, leading to more robust and impactful findings.
Wheter you're working on industrial applications or exploring the biomedical potential of Ethanolamine, PubCompare.ai's intuitive tools and data-driven insights can be invaluable in enhancing your research and development efforts.