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Ethinyl Estradiol

Ethinyl Estradiol is a synthetic estrogen compound widely used in oral contraceptives and hormone replacement therapy.
It is highly potent and effective in regulating the female reproductive system.
Ethinyl Estradiol works by suppressing ovulation and altering the uterine lining to prevent pregnancy.
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This can enhance reproducibility and accuracy in Ethinyl Estradiol research, optimizing the drug discovery and development process.
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Most cited protocols related to «Ethinyl Estradiol»

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Publication 2018
Acetate ammonium fluoride Biological Factors Charcoal Estradiol Ethinyl Estradiol etonogestrel Homo sapiens Levonorgestrel Methanol Methylene Chloride Norethindrone Progesterone Serum
Adult (3 months old) female Sprague–Dawley rats were monitored for estrous cycles with daily vaginal smears. Only rats with at least two regular 4- to 5-day estrous cycles were included in the studies. The females were caged with proven males, and pregnancy was verified by observation of a sperm plug, which was designated as day 0 of pregnancy. Dams were randomly assigned to the following drug conditions: LNG (20 μg levonorgestrel), EE (10 μg ethinyl estradiol), or LNG/EE (20 μg LNG and 10 μg EE combination), and VEH rats received the same volume of vehicle. Drugs were suspended in 5% ethanol in organic sesame oil, and 0.1 ml were given daily through subcutaneous injection at the nape starting from day 1 until pup delivery for ~21 days. The same individuals handled the rats for injections, and experimenters were naive to drug conditions. The male and female offspring were separated from the dams on day 21 and fed until 9–10 weeks old for further experiments. Parts of 9- to 10-week-old offspring were then used for behavior tests, including autism-like and anxiety-like behavior testing. After that, the offspring were sacrificed, and the different brain tissues, including the amygdala, hypothalamus, and hippocampus, were isolated, flash frozen in dry ice, and then stored in a −80 °C freezer for the analysis of gene expression, SOD2 activity, superoxide anion release, DNA damage, mitochondrial function, and fatty acid metabolism. In addition, other parts of the offspring were used for the isolation of primary amygdala neurons.
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Publication 2017
Adult Amygdaloid Body Autistic Disorder Behavior Test Brain DNA Damage Dry Ice Estrous Cycle Ethanol Ethinyl Estradiol Fatty Acids Females Freezing Gene Expression Profiling Hypothalamus isolation Levonorgestrel Males Metabolism Mitochondria Neurons Obstetric Delivery Pharmaceutical Preparations Pregnancy Rats, Sprague-Dawley Rattus Seahorses Sesame Oil SOD2 protein, human Sperm Subcutaneous Injections Superoxides Tissues Vaginal Smears

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Publication 2012
Adult Animals Brain Breast Feeding Cognition Corn oil Diet Dietary Exposure Estrous Cycle Ethinyl Estradiol Females Humidity isolation Light Males Mice, Deer pathogenesis Patient Holding Stretchers Peromyscus Phytoestrogens Polypropylenes Pregnancy Quarantine Reproduction Rodent Sex Differentiation Sexual Maturation Sibling Treatment Protocols Woman Zoonoses
All compounds were purchased from Sigma-Aldrich and included: gambogic acid, sodium salinomycin, ethinyl estradiol, fluoxetidine hydrochloride, bepridil, ciclopirox, miconazole nitrate, chlorpromazine hydrochloride, amphotericin b, niclosamide, rescinnamine, flucytosine, vinblastine, carbidopa, praziquantel and auranofin.
Stock solutions of all compounds were made up at 1 mM in appropriate solvents (Dataset S1) and stored at −80°C. All compounds were added to black-sided, flat-bottom (optically clear), 96-well microtiter plates containing schistosomula (1000 parasites/well in triplicate) at 10 µM concentrations. Schistosomula were cultured (as already indicated; 37°C, 5% CO2) in the presence of each compound for 24 hr before viability levels were assessed.
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Publication 2010
Amphotericin B Auranofin Bepridil Carbidopa Ciclopirox Ethinyl Estradiol Flucytosine gambogic acid Hydrochloride, Chlorpromazine Niclosamide Nitrate, Miconazole Parasites Praziquantel rescinnamine salinomycin Sodium Solvents Vinblastine

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Publication 2012
Acetate Adolescent African American Contraceptive Agents Contraceptive Methods Contraceptives, Oral Desogestrel dienogest drospirenone Ethics Committees, Research Ethinyl Estradiol ethynodiol Gestodene Hispanics Hormones Levonorgestrel Norethindrone norgestimate Norgestrel Pregnancy Progestins Sterility, Reproductive Treatment Protocols Woman Young Adult

Most recents protocols related to «Ethinyl Estradiol»

The serum testosterone level of mice was determined using a competitive binding Testosterone Parameter Assay Kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions with testosterone as the standard. The minimum detectable dose of testosterone for the ELISA kit was approximately 0.03 ng/mL. The following compounds were tested for their cross-reactivity (testosterone cross-reactivity was set as 100%): DHT (2.6%), AD (<0.1%), 17β-estradiol (<0.1%), and progesterone (<0.1%). On the other hand, the serum estradiol level of mice was determined using a competitive binding Estradiol Parameter Assay Kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions with 17β-estradiol as the standard. The minimum detectable dose of estradiol was approximately 5 pg/mL. The following compounds were tested for cross-reactivity (17β-estradiol cross-reactivity was set as 100%): estrone (0.26%), estriol (0.86%), 17α-ethinylestradiol (<0.1%), and progesterone (<0.1%).
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Publication 2023
Biological Assay Cross Reactions Enzyme-Linked Immunosorbent Assay Estradiol Estriol Estrone Ethinyl Estradiol Mice, House Progesterone Serum Testosterone
Denitrifying strain GDN1 (500 mL, initial OD600 nm = 0.2) was incubated at 37°C and pH 6.0 with testosterone (1 mM) and nitrate (10 mM) in a chemically defined medium as described elsewhere31 (link). 17α-Ethinylestradiol (final concentration = 50 μM), which cannot be utilized by strain GDN1, was added to the bacterial culture to serve as an internal control. Cultural samples (5 mL each) were withdrawn every 8 h (0 ~ 48 h). The pH of the cultural samples (1 mL) was adjusted to pH < 2 using 5 M HCl. The acid-treated cultures were extracted three times with the same volume of ethyl acetate to recover the residual testosterone and its derivatives from the aqueous phase. The testosterone-derived intermediates extracted from the cultural samples were identified and quantified using UPLC – HRMS. The protein and nitrate contents of strain GDN1 cultures were determined as described below. The remaining cultural samples (1 mL) were extracted with ethyl acetate three times. After the solvent was completely evaporated, the residue was re-dissolved in 10 μL of dimethyl sulfoxide (DMSO) to determine its androgenic activity (see below).
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Publication 2023
Acids Androgens Bacteria derivatives Ethinyl Estradiol ethyl acetate Nitrates Proteins Solvents Strains Sulfoxide, Dimethyl Testosterone
Strain GDN1 was aerobically grown in a phosphate-buffered shake-flask (300 ml in 1 L-Erlenmeyer flask) containing 1 mM testosterone. 17α-Ethinylestradiol (50 μM; indigestible by strain GDN1) was added as an internal control. Nitrate was omitted from the medium. In 1 L of distilled water, the medium contained the following: 0.29 g testosterone, 2.0 g NH4Cl, 0.5 g MgSO47 H2O, and 0.1 g CaCl22 H2O. After autoclaving, sterile 50 ml KH2PO4-K2HPO4 buffer solution (1 M, pH 6.0), vitamins (1 mL/L)54 (link), EDTA-chelated mixture of trace elements (1 mL/L)55 (link), and selenite and tungstate solution (1 mL/L)56 (link) were added. The aerobic culture was incubated at 37°C in an orbital shaker (180 rpm). The cultural samples (5 mL) were withdrawn every 6 h (0 ~ 30 h). The testosterone-derived intermediates extracted from the cultural samples were identified and quantified using UPLC – HRMS The androgenic activity of the cultural samples was determined as described later.
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Publication 2023
1-testosterone Androgens Bacteria, Aerobic Buffers Edetic Acid Ethinyl Estradiol Nitrates Phosphates potassium phosphate, dibasic Selenite Sterility, Reproductive Strains Testosterone Trace Elements Tremor tungstate Vitamins
The [2,3,4C-13C]testosterone was purchased from Isosciences. Commercially available steroid standards [androstadienedione (ADD), androstenedione (AD), cholesterol, dihydrotestosterone (DHT), estradiol, 17α-ethinylestradiol, progesterone, and testosterone] were purchased from Sigma-Aldrich (St. Louis, MO, USA). The androgenic ring-cleaved metabolites 3,17-DHSA and 2,3-SAOA were produced as described elsewhere29 (link). Other chemicals used were of analytical grade and were purchased from Mallinckrodt Baker (Phillipsburg, USA), Merck Millipore (Burlington, USA), and Sigma-Aldrich unless specified otherwise.
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Publication 2023
Androgens Androstenedione Cholesterol Dihydrotestosterone Estradiol Ethinyl Estradiol Progesterone Steroids Testosterone
Arginine and glucagon tests were the only GH stimulation tests used in our institution during the study period. Protocols for the arginine and glucagon stimulation tests used in our institution are available at https://mft.nhs.uk/app/uploads/2022/03/Paediatric-DFT-Protocols-V7_Feb-2022.pdf (accessed 22nd August 2022). Sex steroid priming is given in our unit for pre-pubertal girls >8 years or boys >9 years undergoing stimulation tests. Ethinylestradiol 10-20 micrograms is given once daily for 3 days prior to the test for both boys and girls. A normal test result in our institution is indicated by a peak GH level ≥ 7 μg/L. GH, IGF-I and IGFBP-3 were measured on the IDS iSYS assay (Immunodiagnostic systems, Tyne and Wear, UK). The GH assay used is standardized to the recombinant GH calibration standard World Health Organization 98/574 and complies with recommendations on assay standardization (19 (link)).
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Publication 2023
Arginine Biological Assay Boys Ethinyl Estradiol Glucagon Gonadal Steroid Hormones IGF1 protein, human IGFBP3 protein, human Immunodiagnosis Puberty Woman

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17α-ethinylestradiol is a synthetic estrogen compound used in various laboratory applications. It serves as a reference standard and is commonly employed in analytical procedures, research, and pharmaceutical development. The core function of this compound is to provide a reliable and consistent reference point for the study and analysis of related substances.
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Ethinyl estradiol is a synthetic estrogen compound commonly used as a pharmaceutical ingredient. It is a white or almost white, crystalline powder that is practically insoluble in water, sparingly soluble in ethanol, and soluble in acetone. Ethinyl estradiol is primarily used in the production of various hormonal medications, such as oral contraceptives and hormone replacement therapies.
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17β-estradiol is a natural estrogen hormone produced by the ovaries, adrenal glands, and other tissues in the body. It is a key component in various laboratory and research applications, serving as a substrate, reference standard, or analytical tool for the study of estrogen-related processes and pathways.
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17α-ethynylestradiol is a synthetic estrogen used as a reference standard in analytical procedures. It is a white to pale yellow crystalline powder with the molecular formula C20H24O2. The compound is commonly used in research and development applications for the identification and quantification of estrogenic substances.
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More about "Ethinyl Estradiol"

Ethinyl Estradiol, 17α-ethinylestradiol, Bisphenol A, 17β-estradiol, 17α-ethynylestradiol, Formic acid, Estrone, DMSO, Acetonitrile, Oral contraceptives, Hormone replacement therapy, Suppressing ovulation, Uterine lining, Drug discovery, Drug development, Reproducibility, Accuracy, Research optimization, PubCompare.ai