> Chemicals & Drugs > Organic Chemical > Ethylenediamines

Ethylenediamines

Ethylenediamines are a class of organic compounds featuring two amino groups attached to an ethylene backbone.
These versatile molecules find applications in a wide range of fields, including chemical synthesis, materials science, and pharmaceutical research.
PubCompare.ai's AI-powered platform can help researchers quickly identify the most effective ethylenediamine protocols from literature, preprints, and patents, optimiizing the efficiency of your research.
Experence the power of AI-assisted ethylenediamine discovery today!

Most cited protocols related to «Ethylenediamines»

Surface Modification of Graphene Oxide for Cryo-EM

Cited 13 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2019

Amines Anabolism Biopharmaceuticals Epoxy Resins Ethanol Ethylenediamines Sulfoxide, Dimethyl

Crystallization of mycobacterial LDT2 protein

Cited 8 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2012

Chromatography, Affinity Crystallization Diffusion Ethylenediamines Gel Chromatography HEPES Iodine Methionine Proteins Sepharose Strains Succinic Acid

Urea Assay with Modified Reagent

Cited 8 times

Urea reagents were prepared as described in Jung et al. (1975) (link). The final Jung working reagent consisted of 100 mg/L o-phthalaldehyde, 215 mg/L N-(1-naphthyl)ethylenediamine, 2.5 mol/L sulfuric acid, 2.5 g/L boric acid, and 0.03% Brij-35. The modified reagent used 513 mg/L primaquine bisphosphate in place of the 215 mg/L N-(1-naphthyl)ethylenediamine reagent. The urea standard was prepared in double-distilled water and contained 5.00 mg/dL urea. To perform the assay, 50 μL of water, 50 μL of the 5.00 mg/dL standard, and 50 μL samples were transferred into separate wells of a clear flat-bottom 96-well plate. Then to each well, 200 μL of freshly prepared working reagent was added and mixed quickly by gently rocking the plate. The reaction was incubated for 1 h at room temperature. Optical densities (OD) at 430 and 505 nm were measured on the plate reader for assays using the modified reagent and the original Jung reagent, respectively.
The calibration curve (Fig. 1A) shows that the assay is linear between 0.00 and 5.00 mg/dL urea. For calculation of the sample urea concentration, the experimenter can choose either to use the slope of the standard curve or to use a single urea concentration (see below). We found that it is sufficient to use one blank (water) and one single urea concentration (5.00 mg/dL) to calculate the sample urea concentrations. In this work, urea concentration in the sample was calculated from the OD values:

\begin{array}{l} [Urea] = \frac{{OD}_{SAMPLE} - {OD}_{BLANK}}{{OD}_{STANDARD} - {OD}_{BLANK}} \times n \\ \times [Standard] (mg / dL) \end{array}

where OD_SAMPLE, OD_STANDARD, and OD_BLANK are OD_{430 nm} values of the sample, standard, and water blank, respectively. [Standard] is the concentration of the urea standard (5.00 mg/dL or 0.83 mmol/L) and n is the dilution factor. Dilution of samples in distilled water is necessary when sample OD_{430 nm} values are higher than the OD_{430 nm} value for the 5.00 mg/dL urea standard.

Zawada R.J., Kwan P., Olszewski K.L., Llinas M, & Huang S.G. (2009). Quantitative determination of urea concentrations in cell culture medium. Biochemistry and cell biology = Biochimie et biologie cellulaire, 87(3), 541-544.

Publication 2009

Biological Assay boric acid Brij 35 Ethylenediamines o-Phthalaldehyde Primaquine Sulfuric Acids Technique, Dilution Urea Vision

Isolation and Culture of Rat BMSCs

Cited 6 times

BMSCs was isolated as described previously by Blanc et al and Fathi et al.^{17 (link),18 (link)} In brief, after giving ethical consent, 5 (5- to 8-week-old) rats were euthanized with an overdose of ketamine/xylazine and bone marrow contains was flushed with phosphate-buffered saline (PBS) supplemented with 5% fetal bovine serum (FBS) (washing buffer). Bone marrow contents was centrifuged and the cell pellet was re-suspended and was layered over same volume of Ficoll-Paque (Innotrain, Germany) and centrifuged at 850×g for 25 minutes at 4°C. In the following, mononuclear cell layer was collected and was re-suspended in Dulbecco’s modified Eagle’s medium (DMEM) culture medium containing 10% FBS. Cell cultures were incubated in a 37°C incubator and passaged with 0.25% trypsin/ethylene diamine tetra acetic acid (EDTA).^{19 (link)} A general overview of methods steps was described as Figure 1.

Fathi E., Valipour B., Sanaat Z., Nozad Charoudeh H, & Farahzadi R. (2020). Interleukin-6, -8, and TGF-β Secreted from Mesenchymal Stem Cells Show Functional Role in Reduction of Telomerase Activity of Leukemia Cell Via Wnt5a/β-Catenin and P53 Pathways. Advanced Pharmaceutical Bulletin, 10(2), 307-314.

Full text: Click here

Publication 2020

Acetic Acids Bone Marrow Buffers Cell Culture Techniques Cells Culture Media Drug Overdose Eagle Ethylenediamines Fetal Bovine Serum Ficoll Ketamine Phosphates Rattus norvegicus Saline Solution Tetragonopterus Trypsin Xylazine

Thioredoxin Reductase Assay Protocol

Cited 6 times

Purified rat liver TrxR, NADPH, DTNB, menadione, catalase, superoxide dismutase, phosphatase inhibitor (catalog no. P2850, which contains microcystinLR, cantharidin, and (−)-p-bromotetramisole) and protease inhibitor cocktail (catalog no. P2714, which contains 4-(2-aminoethyl)benzenesulfonyl fluoride, E-64, bestatin, leupeptin, aprotinin, and EDTA), and β-actin monoclonal antibody were purchased from Sigma (St. Louis, MO). Mechlorethamine hydrochloride was from Aldrich (Milwaukee, WI). Human TrxR mutant (Sec498Cys) was from Ab Frontier (Seoul, Korea). N-(Biotinoyl)-N′-(iodoacetyl) ethylenediamine (BIAM), Amplex Red reagent, Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum, and penicillin/streptomycin were from Life Technologies (Grand Island, NY). Horseradish peroxidase (HRP)-conjugated streptavidin was from GE Healthcare (Piscataway, NJ). Anti-TrxR1, -TrxR2, and -cytochrome c antibodies were from Santa Cruz (Santa Cruz, CA). Chemiluminescent (ECL) substrate was from Thermo Scientific (Rockford, IL).

Jan Y.H., Heck D.E., Malaviya R., Casillas R.P., Laskin D.L, & Laskin J.D. (2013). Cross-Linking of Thioredoxin Reductase by the Sulfur Mustard Analogue Mechlorethamine (Methyl bis(2-chloroethyl) amine) in Human Lung Epithelial Cells and Rat Lung: Selective Inhibition of Disulfide Reduction but Not Redox Cycling. Chemical research in toxicology, 27(1), 61-75.

Publication 2013

4-bromotetramisole Actins Amplex Red Antibodies Aprotinin Cantharidin Catalase Cytochromes c Dithionitrobenzoic Acid Eagle Edetic Acid Ethylenediamines Fetal Bovine Serum Fluorides Homo sapiens Horseradish Peroxidase leupeptin Liver Mechlorethamine Hydrochloride Monoclonal Antibodies NADP Penicillins Phosphoric Monoester Hydrolases Protease Inhibitors Streptavidin Streptomycin Superoxide Dismutase ubenimex Vitamin K3

Most recents protocols related to «Ethylenediamines»

Synthesis of Methotrexate Conjugates

Not available on PMC !

Example 7

The synthesis of the methotrexate conjugates is described in (FIG. 12). A short ethylene diamine spacer may be introduced between methotrexate and the oxidized lipid. 6-Bromomethyl-pteridine-2,4-diamine trihydrobromide (BPT.HBr, 23) may be purchased from Ube Industries and coupled with intermediate 24 to produce 25. Compound 25 may be deprotected from tert-Boc followed by ester hydrolysis to produce amine terminated methotrexate (26). Reductive amination of 26 with ALDO (PE) or (PC) may be performed as described earlier to produce methotrexate prodrugs (27).

US11872313B2. Prodrug compositions, prodrug nanoparticles, and methods of use thereof (2024-01-16). Washington University [US]. Inventors: Gregory M. Lanza [US], Dipanjan Pan [US].

Full text: Click here

Patent 2024

Amination Amines Anabolism Diamines Esters Ethylenediamines Hydrolysis Lipids Methotrexate Prodrugs Pteridines TERT protein, human

Synthesis of Platinum-Based Anti-Cancer Prodrugs

Not available on PMC !

Example 6

For the synthesis of platinum based anti cancer prodrugs, two approaches may be followed. The first approach (FIG. 10) may involve the preparation of an amine terminated cis platin (9) followed by conjugation with oxidized lipids. The coupling intermediate produced from the amidation reaction of compound 8 with mono Boc-ethylenediamine in presence of HATU/DIPEA, may be subjected to deprotection to produce compound 9. Compound 9 may undergo reductive amination with ALDO PC in methanol to generate cis platin prodrug-1 (10).

A second approach (FIG. 11) may involve the synthesis of an analogue bearing hydrophobically modified chelating diamines. Cis-platin intermediate 16 may be obtained in three steps from compound 13. Intermediate 16 may be subjected to complexation with K₂PtCl₄by maintaining the pH of the resulting solution at pH 6-7. Finally, compound 13 may undergo reductive amination with ALDO (PC) or (PE) to produce cis platin prodrug-2 (18).

US11872313B2. Prodrug compositions, prodrug nanoparticles, and methods of use thereof (2024-01-16). Washington University [US]. Inventors: Gregory M. Lanza [US], Dipanjan Pan [US].

Full text: Click here

Patent 2024

Amination Amines Anabolism CISH protein, human Cisplatin Diamines DIPEA Ethylenediamines Lipids Malignant Neoplasms Methanol Platinum Prodrugs SOCS2 protein, human

Synthesis of N-Boc-Ethylenediamine Derivative

Not available on PMC !

Example 111

[Figure (not displayed)]

A mixture of N-Boc-ethylenediamine (5.6 mL, 35.4 mmol, 1.1 eq.) and saturated NaHCO₃(60 mL) was cooled to 0° C., to which compound 509 (5.00 g, 32.2 mmol, 1.0 eq.) was added in portions. After stirring at 0° C. for 30 min, the reaction was warmed to r.t. and stirred for 1 h. The precipitate was collected by filtration and washed with cold water, then dissolved in EtOAc and washed with brine, dried over anhydrous Na₂SO₄and concentrated to give a white solid (6.69 g, 87% yield).

US11873281B2. Conjugates of cell binding molecules with cytotoxic agents (2024-01-16). HANGZHOU DAC BIOTECH CO., LTD. [CN], None [US], None [CN], None [CN]. Inventors: R. Yongxin Zhao [US], Yue Zhang [CN], Yourang Ma [CN].

Full text: Click here

Patent 2024

Anabolism Bicarbonate, Sodium brine Cold Temperature Ethylenediamines Filtration

Paclitaxel Prodrug Synthesis and Purification

Not available on PMC !

Example 4

Paclitaxel prodrug was synthesized following a five-step synthesis process and purified by column chromatography (FIG. 8). Briefly, commercially available paclitaxel (Avachem Scientific, Inc.) was treated with succinic anhydride in the presence of pyridine to give 2′-succinyl paclitaxel. NHS ester (6) of this intermediate may be obtained by reaction with N-succinimidyl diphenylphosphate (SDPP). The ester was treated with an excess of mono-Boc-ethylene diamine at low temperature followed by deprotection of the tert-Boc. A flexible, linear diamine (1,3-diamino propane) was chosen to reduce the steric hindrance. Finally, the paclitaxel amine was subjected to reductive amination with ALDO PC (or PE) to produce paclitaxel prodrugs (7).

US11872313B2. Prodrug compositions, prodrug nanoparticles, and methods of use thereof (2024-01-16). Washington University [US]. Inventors: Gregory M. Lanza [US], Dipanjan Pan [US].

Full text: Click here

Patent 2024

Amination Amines Anabolism Chromatography Cold Temperature Diamines Esters Ethylenediamines Paclitaxel Prodrugs Propane Pyridines succinic anhydride TERT protein, human

TEMPO-Oxidized Cellulose Nanofibrils Production

The bleached
pulp was oxidized at 1 wt % consistency, at pH 10, with 5 mmol of
NaClO as the spent oxidizer, and with 0.1 g of NaBr and 0.016 g of
TEMPO per gram of fiber as co-catalysts, as described in previous
works.^{30 (link),31 (link)} The carboxyl content of the oxidized pulp,
once thoroughly washed with distilled water, accounted for 0.73 ±
0.01 mmol −COOH g^–1, as estimated by Davidson’s
methylene blue adsorption method.^{32 (link)} Its
intrinsic viscosity, measured by the capillary viscometer procedure
(TAPPI T 230 om-08),³³ was 2.37 dL g^–1. From the Mark–Houwink parameters for cellulose
in copper(II) ethylenediamine, as reported elsewhere,^{34 (link),35 (link)} this corresponds to a degree of polymerization of 390.
Fibrillation
was carried out in a high-pressure homogenizer, NS1001L PANDA 2 K-GEA
(GEA Niro Soavi, Parma, Italy). A suspension of oxidized fibers was
passed three times at 300 bar, three times at 600 bar, and three times
at 900 bar. A 0.1 wt %, suspension of the resulting CNFs exhibited
transmittance at 600 nm of 68%.

Aguado R.J., Mazega A., Fiol N., Tarrés Q., Mutjé P, & Delgado-Aguilar M. (2023). Durable Nanocellulose-Stabilized Emulsions of Dithizone/Chloroform in Water for Hg2+ Detection: A Novel Approach for a Classical Problem. ACS Applied Materials & Interfaces, 15(9), 12580-12589.

Full text: Click here

Publication 2023

Adsorption A Fibers Capillaries Copper Dental Pulp Ethylenediamines Fibrosis Polymerization Pressure Viscosity

Top products related to «Ethylenediamines»

Ethylenediamine by Merck Group

Sourced in United States, Germany, United Kingdom, Canada, Spain, Italy, Switzerland, China, Czechia, India

Ethylenediamine is a colorless, flammable liquid with a characteristic ammonia-like odor. It is commonly used as a building block for the synthesis of various chemical compounds in laboratory settings.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal

Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Dimethyl sulfoxide (dmso) by Merck Group

Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh

DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.

Sodium hydroxide (naoh) by Merck Group

Sourced in Germany, United States, India, United Kingdom, Italy, China, Spain, France, Australia, Canada, Poland, Switzerland, Singapore, Belgium, Sao Tome and Principe, Ireland, Sweden, Brazil, Israel, Mexico, Macao, Chile, Japan, Hungary, Malaysia, Denmark, Portugal, Indonesia, Netherlands, Czechia, Finland, Austria, Romania, Pakistan, Cameroon, Egypt, Greece, Bulgaria, Norway, Colombia, New Zealand, Lithuania

Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.

Hydrochloric acid (hcl) by Merck Group

Sourced in Germany, United States, United Kingdom, India, Italy, France, Spain, Australia, China, Poland, Switzerland, Canada, Ireland, Japan, Singapore, Sao Tome and Principe, Malaysia, Brazil, Hungary, Chile, Belgium, Denmark, Macao, Mexico, Sweden, Indonesia, Romania, Czechia, Egypt, Austria, Portugal, Netherlands, Greece, Panama, Kenya, Finland, Israel, Hong Kong, New Zealand, Norway

Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.

Bovine serum albumin (bsa) by Merck Group

Sourced in United States, Germany, United Kingdom, China, Italy, Japan, France, Sao Tome and Principe, Canada, Macao, Spain, Switzerland, Australia, India, Israel, Belgium, Poland, Sweden, Denmark, Ireland, Hungary, Netherlands, Czechia, Brazil, Austria, Singapore, Portugal, Panama, Chile, Senegal, Morocco, Slovenia, New Zealand, Finland, Thailand, Uruguay, Argentina, Saudi Arabia, Romania, Greece, Mexico

Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.

Methanol by Merck Group

Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan

Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.

Sulfanilamide by Merck Group

Sourced in United States, Germany, India, Sao Tome and Principe, United Kingdom, Japan, Portugal, Australia, Switzerland, Spain

Sulfanilamide is a chemical compound used in laboratory settings. It is a white, crystalline powder with a melting point of 165-167°C. Sulfanilamide is a sulfonamide drug that has been used as a bacteriostatic agent. Its core function is to inhibit the growth and reproduction of certain bacteria.

N 1 naphthyl ethylenediamine by Merck Group

Sourced in United States, Germany

N-(1-naphthyl) ethylenediamine is a chemical compound used as a reagent in analytical chemistry. It is a colorless crystalline solid that is soluble in water and organic solvents. The compound is commonly used in the detection and quantification of various analytes, particularly in colorimetric assays.

Ethanol by Merck Group

Sourced in Germany, United States, United Kingdom, Italy, India, France, China, Australia, Spain, Canada, Switzerland, Japan, Brazil, Poland, Sao Tome and Principe, Singapore, Chile, Malaysia, Belgium, Macao, Mexico, Ireland, Sweden, Indonesia, Pakistan, Romania, Czechia, Denmark, Hungary, Egypt, Israel, Portugal, Taiwan, Province of China, Austria, Thailand

Ethanol is a clear, colorless liquid chemical compound commonly used in laboratory settings. It is a key component in various scientific applications, serving as a solvent, disinfectant, and fuel source. Ethanol has a molecular formula of C2H6O and a range of industrial and research uses.

What are the key applications of Ethylenediamines?

Ethylenediamines are versatile organic compounds with a wide range of applications. They are commonly used in chemical synthesis, material science, and pharmaceutical research. In chemical synthesis, ethylenediamines serve as building blocks for the production of various compounds, including pharmaceuticals, dyes, and polymers. In materials science, they are employed in the development of advanced materials, such as metal-organic frameworks and coordination polymers. In the pharmaceutical industry, ethylenediamines are involved in the synthesis of drug molecules and the formulation of drug delivery systems.

What are the different types or variations of Ethylenediamines?

Ethylenediamines can exist in various forms, including aliphatic, aromatic, and heterocyclic variations. The most common types include ethylenediamine (en), diethylenetriamine (dien), and triethylenetetramine (trien). These compounds differ in the number of amino groups and the length of the ethylene backbone, which can impact their reactivity, solubility, and specific applications.

What are some common challenges in working with Ethylenediamines?

One of the main challenges in working with ethylenediamines is their reactivity. These compounds can readily undergo side reactions, such as oxidation, hydrolysis, and complexation with metal ions. Proper handling and storage conditions are essential to maintain the integrity of ethylenediamine-based products. Additionally, the solubility and compatibility of different ethylenediamine variants can vary, requiring careful selection and optimization of reaction conditions.

How can PubCompare.ai help in optimizing Ethylenediamine research?

PubCompare.ai's AI-powered platform can significantly streamline your Ethylenediamine research. The tool allows you to efficiently screen protocol literature, leveraging advanced AI algorithms to pinpoint critical insights. By comparing the effectiveness of different Ethylenediamine protocols from literature, preprints, and patents, PubCompare.ai can help you identify the most optimal methods for your specific research goals. This can save you time, improve reproducibility, and enhance the accuracy of your Ethylenediamine-based experiments and studies.

How can PubCompare.ai assist in identifying the best Ethylenediamine protocols?

PubCompare.ai's AI-driven analysis can highlight key differences in the effectiveness of Ethylenediamine protocols, enabling you to choose the best option for your research. The platform's advanced comparisons can pinpoit critical insights, such as the impact of different reaction conditions, reagents, and purification techniques on the performance of Ethylenediamine-based products. This information can be invaluable in selecting the most reproducible and accurate protocols, saving you time and effort in your Ethylenediamine research.

More about "Ethylenediamines"

Ethylenediamines are a versatile class of organic compounds featuring two amino groups attached to an ethylene backbone.
These molecules have a wide range of applications in chemical synthesis, materials science, and pharmaceutical research.
PubCompare.ai's AI-powered platform can help researchers quickly identify the most effective ethylenediamine protocols from literature, preprints, and patents, optimizing the efficiency of your research.
Ethylenediamines are closely related to other important chemical compounds like FBS (Fetal Bovine Serum), DMSO (Dimethyl Sulfoxide), and Sodium Hydroxide.
These compounds are often used in conjunction with ethylenediamines in various research and industrial applications.
Hydrochloric Acid, Bovine Serum Albumin, Methanol, Sulfanilamide, and N-(1-naphthyl) Ethylenediamine are also commonly associated with ethylenediamine-based processes and experiments.
Utilizing the power of artificial intelligence, PubCompare.ai's platform can assist researchers in navigating the vast amount of literature and data related to ethylenediamines.
By quickly identifying the most effective protocols and methods, researchers can save time and effort, ultimately accelerating their discoveries and innovations in this field.
Experence the power of AI-assisted ethylenediamine research today!