The largest database of trusted experimental protocols

Eucalyptol

Eucalyptol is a colorless, volatile, aromatic compound found in the essential oils of various Eucalyptus species.
It has a wide range of potential applications, including use as a flavoring agent, a fragrance compound, and a topical analgesic.
Eucalyptol exhibits antimicrobial, anti-inflammatory, and expectorant properties, making it a subject of interest in medical and pharmaceutical research.
Identifiying the most effective protocols and products for utilizing eucalyptol can streamline research and lead to data-driven decisions, as PubCompare.ai's platform aims to facilitate.

Most cited protocols related to «Eucalyptol»

We assumed that meaningful conclusions could be obtained by analysing 30 products. The e-cigarette fluids examined were selected from a vast and rapidly changing array of products. BLU and NJOY, two brands of disposable-cartridge e-cigarettes, were purchased in five flavours: tobacco, menthol, vanilla, cherry and coffee. Also purchased in the same flavours (from online retailers and local ‘vape’ shops in Portland, Oregon) were refill bottles for tank systems. Refill bottles in five other confectionary flavours (chocolate/cocoa, grape, apple, cotton candy and bubble gum) were also purchased. After dilution with methanol, the fluids were analysed by GC/MS. Using internal standard-based calibration procedures similar to those described elsewhere,16 (link) analyses were performed using an Agilent (Santa Clara, California, USA) 7693 autosampler, Agilent 7890A GC and Agilent 5975C MS. The GC column type was Agilent DB-5MS UI, of 30 m length, 0.25 mm id and 0.25 mm film thickness. For each replicate sample, ∼50 mg of each fluid was dissolved in 1 mL of methanol. One microlitre of the methanol solution was then injected on the GC with a 25:1 split. The GC temperature programme for all analyses was: 35°C hold for 5 min; 10°C/min to 300°C; then hold for 3.5 min at 300°C. No analyses of aerosols generated from the fluids were carried out.
Qualitative analyses of the 30 e-cigarette fluids were first carried out here using the NIST 14 MS library,17 and the results were compared with data previously obtained for flavoured tobacco products.16 (link) Quantitative analyses of the 30 fluids were then undertaken, using authentic standards, for a specific list of compounds, which formed the ‘target analyte list’. If reported here, the presence of each target analyte was confirmed by matching GC retention times and MS patterns with results obtained with the authentic standards; the level was determined by comparison with calibration standard runs. The target analyte list included the 70 compounds listed in Brown et al16 (link) plus 20 others, namely aromadendrene, 1,4-cineol, trans-cinnamaldehyde, citronellal, citronellyl propionate, coumarin, decanal, ethyl acetate, ethyl hexanoate, fenchol, limonene oxide, trans-linalyl propionate, maltol, 3′-methylacetophenone, neomenthol, 2-nonanone, pentyl propionate, pulegone, γ-terpineol and 2,3,5,6-tetramethylpyrazine. The vicinal diketone compounds diacetyl and 2,3-pentanedione were not in the target analyte list.
Publication 2015
2-nonanone 3,7-dimethyl-1,6-octadien-3-yl propionate Aerosols aromadendrene Cacao Candy cDNA Library cinnamic aldehyde citronellal Coffee coumarin decanal Diacetyl DNA Replication ethyl acetate ethyl caproate Eucalyptol fenchol Gas Chromatography-Mass Spectrometry Gossypium Grapes limonene oxide maltol Menthol Methanol Propionate Prunus cerasus pulegone Retention (Psychology) Technique, Dilution tetramethylpyrazine Tobacco Products Vanilla VAPE protocol
The induction of apoptosis activity of A. membranacea EO and 1,8-cineole was investigated by annexin V FITC/Propidium iodide protocol as described in the literature [44 (link)]. Briefly, A2780 cells were seeded at 1 × 105 cells/well in 6-well plate overnight before treatment with either A. membranacea EO or 1,8-cineole at four concentrations, ranging from 0 to 50 μg/mL, or 0–1 µM, respectively (representing IC50: 0, ×1, ×2 and ×4). Following treatment, cells were collected (including the supernatant), washed with ice-cold PBS and then incubated for 2 min in binding buffer (100 µL) and annexin V FITC (10 μL), at room temperature in the dark. Additional 400 μL of binding buffer and 10 μL PI were then added. The early apoptotic, late apoptotic, and necrotic cell populations were analyzed by flow cytometry (FC500, Beckman Coulter, Brea, CA, USA), based on 20,000 events per sample.
Full text: Click here
Publication 2020
Apoptosis Buffers Cells Cold Temperature Eucalyptol FITC-annexin A5 Flow Cytometry Necrosis Population Group Propidium Iodide
The
full-length genes coding for 1,8-Cineole synthase (bCinS; WP_003952918)
and Linalool synthase (bLinS; WP_0003957954) from Streptomyces
clavuligerus
ATCC 27064 were codon optimized and synthesized
from GeneArt (Life Technologies). The genes were amplified using PCR
and subcloned into pETM11 vector digested with NcoI and XhoI using
Infusion cloning (Clontech). The final construct coded for either
1,8-Cineole synthase (bCinS) or Linalool synthase (bLinS) with a 6X-His
tag followed by a TEV protease cleavage site at the N-terminus. The
expression and purification method explained below was identical for
both the proteins. The plasmid was transformed into E. coli ArcticExpress (DE3) cells (Agilent), and
a few colonies were inoculated into 100 mL of 2X-YT media containing
40 μg/mL of kanamycin and 20 μg/mL of gentamycin and grown
for 3–4 h at 37 °C. The culture was diluted
into 3 L of fresh 2X-YT media containing 40 μg/mL of kanamycin
and allowed to grow at 37 °C until the OD at 600
nm reached 0.6–0.8. At this stage, the temperature was reduced
to 16°C and 0.1 mM Isopropyl β-D-1-thiogalactopyranoside
(IPTG) was added and incubated for 14–18 h. The cells were
harvested by centrifugation at 6000g for 10 min,
and the pellet was resuspended in buffer A (25 mM Tris pH 8.0, 150
mM NaCl, 1 mM DTT, 4 mM MgCl2, and 5% (v/v) glycerol).
The cells were lysed by sonication, and the debris was removed by
centrifugation at 30 000g for 30 min. The
supernatant was filtered through a 0.2 μm filter and loaded
onto a 5 mL HisTrap column (GE Healthcare) pre-equilibrated with buffer
A. The column was washed with buffer A containing 10 mM imidazole
(pH 8.0) and increasing up to 40 mM imidazole by step gradients with
3 column volume for each concentration. Increasing the concentration
of imidazole to 200–500 mM eluted the protein. The purified
protein was desalted using a Centripure P100 column (emp Biotech GmbH)
equilibrated with buffer A. To remove the His tag, TEV protease was
added (1:1000 (w/w)) to the protein and incubated at 4 °C with gentle mixing for 24 h. The TEV protease was removed by passing
the protein mixture through a 5 mL HisTrap column, and the flow through
was collected. The His-tag removed protein was concentrated and loaded
onto a Hiload Superdex (26/60) S75 column (GE Healthcare) pre-equilibrated
with buffer A. Pure fractions from the gel filtration column were
concentrated to 13–15 mg/mL and stored at −80 °C as aliquots. Samples for EPR experiments were prepared
as explained above except buffer A was lacking MgCl2.
Publication 2017
Buffers Cells Centrifugation Cloning Vectors Codon Cytokinesis Escherichia coli Eucalyptol Gel Chromatography Genes Gentamicin Glycerin imidazole Isopropyl Thiogalactoside Kanamycin linalool Magnesium Chloride Nitric Oxide Synthase Plasmids Proteins Sodium Chloride TEV protease TPX2 protein, human Tromethamine

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2015
Aluminum Chloride arteannuin B artemetin artemisia ketone artemisic acid Artemisinins Camphor casticin cDNA Library Chlorogenic Acid chrysoplenetin chrysoplenol D deoxyartemisinin dihydroartemisinic acid Eucalyptol eupatorin Flavonoids Gas Chromatography-Mass Spectrometry Gifts kaempferol Luteolin Mannans nerolidol Quercetin rosmarinic acid Scopoletin
These experiments made use of the well known digging task used to study olfactory memory (Eichenbaum, 1998 (link)), in which rats are trained to dig in cups of odorized bedding material to retrieve buried food rewards (45 mg sucrose pellets, Bioserve, Inc., Frenchtown, NJ). All of the rats were first trained on one list of odor pairs. They were then given either muscimol or saline infusions and training on a second list of odors either in the same context or a different context. Thus, the experimental manipulations took place during training on the second list in a 2X2 design with lesion condition (saline or muscimol) and context condition (same or different) as factors.
The two contexts differed along the following dimensions: color of the chamber (white or black), color of the curtains surrounding the training area (black or white), substrate in the chamber (uncovered Plexiglass floor or a black rubber mat), the 65 dB continuous background masking noise (white noise or pink noise) and the ambient odor left by wiping out the chamber with baby wipes prior to each training session (unscented or scented, Rite Aid, Inc). Additionally, the rats were transported in covered cages to the experimental area by different methods in the two contexts (via a cart or carried by hand).
The rats were trained in Plexiglas chambers (45 cm wide X 60 cm long X 40 cm deep) equipped with a removable divider, which separated the odor presentation area from an area where the rats waited during the intertrial interval. Odor cues were presented in ceramic dessert cups (8.25cm in diameter, 4.5cm deep). The digging cups fit into circular cutouts cemented to the floor of the chamber to discourage the rats from moving the cups or tipping them over. Training was carried out in a circular area (2.7m in diameter) enclosed by curtains.
Thirty-two pure odorants served as cues. The amount of each odorant was calculated so that they produced an equivalent vapor phase partial pressure when mixed with 50 ml of mineral oil (Cleland et al., 2002 (link)). 10 ml of each odorant solution was then mixed with 2 liters of corncob bedding material and stored in covered containers. The odors included: propyl butyrate, citronellal, ethyl isovalerate, furfuryl proprionate, n-butyl glycidyl ether, methyl salicylate, n-amyl acetate, ethyl butyrate, propionic acid, benzaldehyde, 1-octanol, pentanol, trans-2-hexenyl acetate, propenoic acid, heptanol, ethyl valerate, 1,8-cineole, anisole, 5-methylfurfural, ethyl acetate, (+/−) limonene, methyl butyrate, 2-phenylethanol, 1-butanol, methyl 2-furoate, butyl butyrate, cis-3-hexenyl acetate, pentyl butyrate, benzyl benzoate, 2-furyl methyl ketone, 1-nonanol, and butyl pentanoate.
Publication 2011
1-nonanol 1-Octanol 5-methyl-2-furfural Acetate Acetone Acids amyl acetate anisole benzaldehyde benzyl benzoate black rubber Butyl Alcohol Butyrates CART protein, human cis-3-hexenyl acetate citronellal ethyl acetate ethyl butyrate ethyl isovalerate ethyl valerate Eucalyptol Food Heptanol Infant Limonene Memory methyl butyrate methyl salicylate Muscimol n-butyl glycidyl ether n-butyl n-butyrate Odorants Odors Oil, Mineral Pellets, Drug Phenylethyl Alcohol Plexiglas propionic acid Rattus norvegicus Saline Solution Sense of Smell Sucrose Valerates Vapor Pressure

Most recents protocols related to «Eucalyptol»

In this study, we first used terpene synthase protein sequences from fully sequenced genomes of A. thaliana100 and E. grandis29 (link), to classify the putative genes found in P. cattleyanum according to the previous classification in the subfamilies TPS-a,-b,-c,-e/f, and -g by sequence similarity26 (link).
To examine the evolutionary history of TPS genes, a second analysis including more species (E. grandis, E. globulus, A. thaliana, P. trichocarpa, V. vinifera, C. citriodora, and M. alternifolia) was carried out. We generated a tree with TPS sequences related to primary metabolism (subfamilies -c, -e, and -f) with a total of 45 sequences and a second tree related to secondary metabolism (subfamilies a, b, g) including 360 sequences29 (link),32 (link),55 (link).
The functionally characterized pinene (RtTPS1 and RtTPS2 accession number AXY92166 and AXY92167, respectively) and caryophyllene synthases (RtTPS3 and RtTPS4 accession numbers AXY92168 and AXY92169) from Rhodomyrtus tomentosa52 (link), pinene synthase (EpTPS1 accession number MK873024) and 1,8-cineole synthases (EpTPS2 and EpTPS3 accession numbers MK873025 and QCQ05478) from Eucalyptus polybractea56 (link), beta cayophyllene synthase (Eucgr. J01451) from E. grandis29 (link), myrcene synthase from Antirrhium majus (AAO41727)101 (link), two isoprene synthase genes from E. globulus (EglobTPS106), E. grandis (Eucgr. K00881)29 (link) and five linalool synthases from Oenothera californica (AAD19841)63 (link), Clarkia breweri (AAD19840), Clarkia concinna (AAD19839), and Fragaria x ananassa (CAD57106)102 (link) were also included in the phylogenetic analysis to assess the homology of known TPS to Psidium genes.
For each dataset used to construct the trees, we first aligned the amino acid sequences of putative TPS genes using ClustalW implemented within MEGA v7.0 software package103 (link). Due to high levels of variation and variable exon counts between taxa, we trimmed the alignment using Gblocks104 (link) with the following parameters: smaller final blocks, gap positions within the final blocks, and less strict flanking positions. We used the maximum-likelihood method implemented in PhyML v2.4.4105 (link) online web server106 (link) to perform the phylogenetic analysis. The JTT + G + F was the best-fit substitution model selected with ModelGenerator for protein analyses107 (link). The confidence values in the tree topology were assessed by running 100 bootstrap replicates. Trees were visualized using Figtree v1.4.4108 .
Full text: Click here
Publication 2023
Amino Acid Sequence caryophyllene Clarkia Eucalyptol Eucalyptus Evolution, Molecular Exons Fragaria Genes Genome isoprene synthase linalool Metabolism myrcene Nitric Oxide Synthase Oenothera Proteins Psidium Secondary Metabolism terpene synthase Trees
The present assessment is based on data submitted by the applicant in the form of a technical dossier9 in support of the authorisation request for the use of laurel leaf oil from L. nobilis as a feed additive.
The FEEDAP Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) used the data provided by the applicant together with data from other sources, such as previous risk assessments by EFSA or other expert bodies, peer‐reviewed scientific papers, other scientific reports and experts' knowledge, to deliver the present output.
Many of the components of the essential oil under assessment have been already evaluated by the FEEDAP Panel as chemically defined flavourings. The applicant submitted a written agreement to reuse the data submitted for the assessment of chemically defined flavourings (dossiers, publications and unpublished reports) for the risk assessment of preparations belonging to BDG 6, including the current one under assessment.10EFSA has verified the European Union Reference Laboratory (EURL) report as it relates to the methods used for the control of the phytochemical markers in botanically defined flavourings from Group 06 – Laurales, Magnoliales, Piperales. During the assessment, upon request from EC and EFSA, the EURL issued two amendments of the original report.11 For the additive under assessment, laurel oil, the evaluation of the method of analysis is included in the second amendment. In particular, for the characterisation of laurel oil the EURL recommended methods based on gas chromatography with flame ionisation detector (GC‐FID) for the quantification of the phytochemical marker 1,8 cineole in laurel oil.12
Full text: Click here
Publication 2023
Eucalyptol Flame Ionization Gas Chromatography Health Risk Assessment Human Body Laurales laurel oil Oils, Volatile Phytochemicals Plant Leaves

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2023
1-Octanol acetophenone benzaldehyde caprylic aldehyde carvone d-Limonene ethyl butyrate Eucalyptol Eugenol geraniol isoamyl acetate Kinetics n-hexanal Obstetric Delivery Odors Sense of Smell
Patients were recruited to the study in the period from June to October 2020 based on the diagnostic criteria of CRS including medical history, physical examination, nasal endoscopy, and computed tomography (CT) scan of the sinuses. Nasal polyp tissue specimens were obtained laterally to the concha nasalis media during endonasal sinus surgery from CRSwNP patients (n = 15) after 14 days of 1,8-Cineol administration as well as from CRSwNP patients (n = 15) without prior 1,8-Cineol administration. Before surgery, all patients had been free of steroid medication for at least four weeks. Fresh tissue samples were flash frozen in liquid nitrogen immediately after resection and stored at − 80 °C.
1,8-Cineol (CNL-1976) was used in terms of the clinically approved drug Soledum® Kapseln forte (capsules) (Cassella-med GmbH & Co. KG, Cologne, Germany). For therapeutic use patients have been prescribed Soledum capsules (3 × 200 mg/day) over 14 days. We analyzed 30 CRSwNP patients (22 men, 8 women) with a mean age of 50.9 years.
Tissue homogenates of nasal polyps from 30 CRSwNP patients were prepared in glass vials using the Omni Tissue Master Homogenizer (Perkin Elmer GmbH, Hamburg, Germany) in ice cold phosphate buffered saline (PBS). The protein concentrations of the samples were determined using Bradford's assay (BioRad Laboratories GmbH, Munich, Germany) following the manufacturer's instructions. Samples were stored at − 80 °C.
Full text: Click here
Publication 2023
Biological Assay Capsule Common Cold Diagnosis Endoscopy Eucalyptol Freezing Nasal Polyps Nitrogen Nose Operative Surgical Procedures Patients Pharmaceutical Preparations Phosphates Physical Examination Proteins Radionuclide Imaging Saline Solution Sinuses, Nasal Soledum Steroids Therapeutic Uses Tissues Turbinates Woman X-Ray Computed Tomography
A sensitive gas chromatography-mass spectrometry (GC–MS) method has been developed and validated for the detection and quantification of 1,8-Cineol in tissue samples of nasal polyps from CRSwNP patients.
The analytes were chromatographically separated on a fused silica column with 95% dimethyl and 5% diphenyl polysiloxane (Rtx-5 Amine; 30 m, 0,25 mm ID, 0,25 µm film-thickness; Restek Corporation, Bellefonte, PA, USA) using a 6890N with MSD 5973 Network gas chromatography mass spectrometry system (Agilent Technologies Inc., Santa Clara, CA, USA). The temperature program used for chromatographic separation is described in Table 1. Helium (5.0, Messer GmbH, Bad Soden am Taunus, Germany) at a constant flow of 1.5 mL/min was used as a carrier gas. Electron ionization (EI) at 70 eV was used at a source temperature of 230 °C. Mass spectrometric determination was performed in selective ion monitoring (SIM) mode, analyzing the masses m/z 154 (used for quantification) and m/z 108 as a qualifier for 1,8-Cineol. Quantifier and qualifier mass transitions for 1,4-Cineol were m/z 111 and m/z 154, respectively. MS quadrupole temperature was 150 °C. A solvent delay of 4 min was used with following acquisition for 10 min.

Temperature program of the gas chromatograph.

Ramp noRate [°C/min]End temperature [°C]Time [min]
Start0502
1201300
2302500
Post-run02902
MassHunter GC/MS Acquisition (B.07.06.2704, Agilent Technologies. Inc. Santa Clara, CA, USA) and MassHunter Workstation Quantitative Analysis (B.09.00) were employed for data acquisition and evaluation.
Full text: Click here
Publication 2023
Amines Chromatography diphenyl Electrons Eucalyptol Gas Chromatography Gas Chromatography-Mass Spectrometry Helium Mass Spectrometry Nasal Polyps Patients Silicon Dioxide Siloxanes Solvents Tissues

Top products related to «Eucalyptol»

Sourced in United States, Germany, Italy, United Kingdom, Brazil
1,8-cineole is a naturally occurring cyclic ether compound. It is a colorless liquid with a characteristic eucalyptus-like odor. 1,8-cineole is commonly used as a reference standard in analytical procedures.
Sourced in United States, Germany, Italy, United Kingdom, Spain, Brazil, Canada, Switzerland, France, Sao Tome and Principe, Japan, Poland, India
α-pinene is a naturally occurring organic compound that is commonly used in laboratory settings. It is a bicyclic monoterpene with the molecular formula C₁₀H₁₆. α-pinene serves as a versatile starting material for various chemical reactions and synthesis processes.
Sourced in United States, Germany, Italy, United Kingdom, Spain, Mexico, China, Brazil, Switzerland, Canada, Czechia
Limonene is a naturally occurring hydrocarbon found in the rinds of citrus fruits. It is commonly used as a solvent in laboratory settings due to its ability to dissolve a wide range of organic compounds.
Sourced in United States, Germany, United Kingdom, Australia
Eucalyptol is a volatile organic compound found in the essential oils of various plants, particularly the eucalyptus tree. It is a colorless, oily liquid with a distinctive camphor-like odor. Eucalyptol is commonly used in the manufacturing of various products, including pharmaceuticals, cosmetics, and cleaning agents, due to its properties as a fragrance, flavoring, and potential therapeutic applications.
Sourced in United States, Germany, Italy, United Kingdom, China, Spain, France, Brazil, Switzerland, Poland, Australia, Hungary, Belgium, Sao Tome and Principe
Linalool is a naturally occurring terpene alcohol found in various plant species. It is a colorless to pale yellow liquid with a floral, citrus-like aroma. Linalool is commonly used as a fragrance ingredient in personal care products and as a flavoring agent in food and beverages. Its core function is as a chemical precursor and intermediate in the synthesis of other compounds.
Sourced in United States, Italy, Germany, United Kingdom, Sao Tome and Principe
γ-terpinene is a naturally occurring organic compound classified as a monoterpene. It functions as a precursor for the synthesis of various chemical compounds used in various industrial and research applications.
Sourced in United States, Germany, Italy, United Kingdom, Brazil, Switzerland, Czechia
P-cymene is a chemical compound used as a laboratory reagent. It is a colorless liquid with a distinctive odor. P-cymene is primarily used as a solvent and in the synthesis of other organic compounds. Its core function is to serve as a versatile chemical intermediate in various laboratory applications.
Sourced in United States, Germany, Italy, Brazil, Japan, United Kingdom, France
α-terpineol is a naturally occurring cyclic monoterpenoid alcohol. It is a colorless to pale yellow liquid with a floral, lilac-like aroma. α-terpineol is commonly used as a fragrance and flavor ingredient in various products.
Sourced in United States, Germany, Italy, United Kingdom, Brazil
Terpinen-4-ol is a naturally occurring organic compound that can be extracted from various plant sources. It is a colorless liquid with a characteristic herbal aroma. Terpinen-4-ol is a terpene alcohol that is commonly used as a reference standard or analytical reagent in laboratory settings.
Sourced in United States, Germany, Italy, Brazil, United Kingdom, Japan
β-pinene is a naturally occurring bicyclic monoterpene hydrocarbon found in the essential oils of various plants. It is a colorless liquid with a characteristic pine-like odor. β-pinene is commonly used as a precursor in the synthesis of various organic compounds and as a component in fragrances and flavors.

More about "Eucalyptol"

Eucalyptol, also known as 1,8-cineole, is a colorelss, volatile, and aromatic compound found in the essential oils of various Eucalyptus plant species.
This versatile compound has a wide range of potential applications, including use as a flavoring agent, a fragrance compound, and a topical analgesic.
Eucalyptol exhibits antimicrobial, anti-inflammatory, and expectorant properties, making it a subject of interest in medical and pharmaceutical research.
Eucalyptol is closely related to other terpene compounds such as α-pinene, Limonene, Linalool, γ-terpinene, P-cymene, α-terpineol, and Terpinen-4-ol, all of which are common constituents of essential oils.
These compounds often work in synergy, contributing to the therapeutic benefits of Eucalyptol and other essential oil blends.
Identifying the most effective protocols and products for utilizing Eucalyptol can streamline research and lead to data-driven decisions.
PubCompare.ai's platform aims to facilitate this process by providing a comprehensive database of protocols from literature, pre-prints, and patents, along with AI-powered comparisons to help researchers and product developers make informed choices.
By leveraging PubCompare.ai's tools, researchers can experience the future of research optimization and make more informed, data-driven decisions regarding the use of Eucalyptol and related terpene compounds.