CRISPR-mediated knockout plasmids containing guide RNAs targeting BAX, BAK1, NCKAP1, ACSL4, SLC7A11, CYFIP1, WAVE-2, Abi2, HSPC300 were generated in lentiCRISPR v2 (Addgene, #52961) according to the standard protocol. The SLC7A11 cDNA–containing expression construct was described in previous publications25 , 26 . The lentiviral construct expressing membrane-bound green fluorescent protein (mGFP) (#22479) and Rac1-Q61L cDNA-containing construct (#84605) were obtained from Addgene. NCKAP1 cDNA and shRNA constructs targeting RPN1, N-WASP, WHAMM were obtained from the Functional Genomics Core Facility of The University of Texas MD Anderson Cancer Center. NCKAP1 and Rac1-Q61L cDNA were subsequently cloned into the vector pLX302 with a C-terminal V5 tag (Addgene, #25896). WAVE-2 constructs were provided by Dr. Daniel D. Billadeau. All constructs were confirmed by DNA sequencing. The sequences of gRNAs and shRNA used in this study are listed in Supplementary Table 4 . Necroptosis inhibitor Nec-1s (#2263) was from BioVision, and necrosis inhibitor Necrox-2 (#ALX-430-166-M001) was from Enzo. Ferroptosis inducer (1S,3R)-RSL3 (#19288) and apoptosis inducer staurosporine (#81590) were from Cayman Chemical. L-[1, 2, 1', 2'-14C]-cystine (#NEC854010UC) was from PerkinElmer. KL-11743 was from Kadmon. The following reagents were obtained from Sigma-Aldrich: 2-deoxy-D-glucose (#D8375-1G), Trolox (#238813), 4-Hydroxy-TEMPO (Tempol) (#176141), beta-mercaptoethanol (2ME) (#M6250), deferoxamine mesylate salt (DFO) (#D9533), ferrostatin-1 (#SML0583), chloroquine (#C6628), diamide (#D3648), diethyl-maleate (#D97703, BAY-876 (#SML1774), and L-Cystine (#C7602). All reagents were dissolved according to manufacturers’ instructions.
>
Chemicals & Drugs
>
Organic Chemical
>
Ferrostatin-1
Ferrostatin-1
Ferrostatin-1 is a small molecule that inhibits ferroptosis, a form of programmed cell death involving iron-dependent lipid peroxidation.
It has been studied for its potential therapeutic applications in various disease models, including neurodegenerative disorders, cancer, and ischemia-reperfusion injury.
Ferrostatin-1 has been shown to protect cells from oxidative stress by blocking the activation of the lipid peroxidation pathway.
Resaerchers can leverage PubCompare.ai's AI-driven platform to optimize their Ferrostatin-1 reserch, locate the best protocols from literature, pre-prints, and patents, and enhance reproducibility and accuary.
It has been studied for its potential therapeutic applications in various disease models, including neurodegenerative disorders, cancer, and ischemia-reperfusion injury.
Ferrostatin-1 has been shown to protect cells from oxidative stress by blocking the activation of the lipid peroxidation pathway.
Resaerchers can leverage PubCompare.ai's AI-driven platform to optimize their Ferrostatin-1 reserch, locate the best protocols from literature, pre-prints, and patents, and enhance reproducibility and accuary.
Most cited protocols related to «Ferrostatin-1»
2-Mercaptoethanol
ABI2 protein, human
Apoptosis
BAK1 protein, human
BAY-876
Caimans
Chloroquine
Cloning Vectors
Clustered Regularly Interspaced Short Palindromic Repeats
Cystine
Diamide
diethyl maleate
DNA, Complementary
Ferroptosis
ferrostatin-1
Glucose
Malignant Neoplasms
Membrane Proteins
Mesylate, Deferoxamine
NCKAP1 protein, human
Necroptosis
Necrosis
oxytocin, 1-desamino-(O-Et-Tyr)(2)-
Plasmids
RNA
Salts
Short Hairpin RNA
Staurosporine
tempol
TEMPOL-H
Trolox C
WASL protein, human
Protocol full text hidden due to copyright restrictions
Open the protocol to access the free full text link
Acetylcysteine
Actins
Antibodies
benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone
Biological Assay
Biological Factors
BODIPY
Cell Survival
CTNNB1 protein, human
cupric chloride
Cycloheximide
DAPI
Deferoxamine
Disulfiram
Esters
ferrostatin-1
GAPDH protein, human
GSK3B protein, human
Helix (Snails)
Immunoglobulins
MG 132
N-Cadherins
NAD(P)H dehydrogenase (quinone) 1, human
NFE2L2 protein, human
Novus
rhodamine B
SB 203580
SCH772984
Sorafenib
SP600125
trigonelline
Vimentin
Protocol full text hidden due to copyright restrictions
Open the protocol to access the free full text link
Argon Ion Lasers
Buffers
Cells
Deferasirox
ferrostatin-1
Flow Cytometry
Fluorescence
fluorexon
Phosphates
prisma
In our study, to evaluate the ferroptosis level in different groups, the MDA, 4-HNE and iron levels were detected in each group. The MDA concentration, 4-HNE concentration and iron concentration in cell lysates were assessed using the Lipid Peroxidation (MDA) Assay Kit (Sigma-Aldrich, Cat #: MAK085), Lipid Peroxidation (4-HNE) Assay Kit (Abcam, Cat #: ab238538) and Iron Assay Kit (Sigma-Aldrich, Cat #: MAK025) according to the manufacturer’s instructions.
Biological Assay
Cells
Ferroptosis
Iron
Lipid Peroxidation
1,2-oleoylphosphatidylcholine
benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone
Biological Assay
Bromides
Caspase Inhibitors
Cell Death
Cells
Cell Survival
Cytotoxin
Ferroptosis
ferrostatin-1
Flow Cytometry
Interleukin-13
Lactate Dehydrogenase
Liposomes
locostatin
necrostatin-1
Phosphotransferases
Plasmids
Promega
Propidium Iodide
RIPK1 protein, human
Most recents protocols related to «Ferrostatin-1»
For cell viability experiments involving cystine restriction, Erastin, or ferrostatin-1, the cells were incubated in white 96-well plates. Specifically, SW480, SW620, and SW837 cells were plated at 8 × 103 cells per well; DLD1 cells were plated at 3 × 103 cells per well. Cells were cultured overnight, transferred to the indicated medium, and incubated for 24 h. Cell viability was evaluated according to the protocol of the Cell Titer-Glo Kit (Promega #G9241). For viability assays, following treatment with Erastin and ferrostatin-1, cells were plated at 5 × 103 cells per well in white 96-well plates, cultured overnight, and then treated with the indicated concentrations of Erastin alone or in combination with 2 μM ferrostatin-1 for 48 h. The viability assays were performed as described above.
After the POF model was successfully established, the EnSCs transplantation group was constructed from day 8 onwards by tail vein injection of EnSCs (passage 3–5, 2 × 106 in 200 µl cell suspensions) (Fig. 4D). At the same time, the control group and the cisplatin group were administered with an equal volume of the medium.
On the other hand, ferrostatin-1 (5 mg/kg) was intraperitoneally administered 1 h earlier than cisplatin for 7 consecutive days as the cisplatin + Ferrostatin-1 group (Fig.S1 A). The solvent for ferrostatin-1 in vivo was 0.1% DMSO. An equal volume of DMSO was used in the control group and cisplatin group. After all processing was completed, blood samples were collected from the mice’s eyeballs. Then they were euthanized by a dislocated cervical spine and bilateral ovaries of mice were harvested.
On the other hand, ferrostatin-1 (5 mg/kg) was intraperitoneally administered 1 h earlier than cisplatin for 7 consecutive days as the cisplatin + Ferrostatin-1 group (Fig.
The utilized reagents in this study included ferrostatin-1 (cat. No.: S7243), Liproxstatin-1 (cat. No.: S7699), Z-VAD-FMK (cat. No.: S7023), Necrosulfonamide (cat. No.: S8251), 3-Methyladenine (cat. No.: S2767), MG132 (cat. No.: S2619), CHX (cat. No.: S7418) were purchased from Selleck Chemicals (Houston, Texas, USA). The stimulation concentration were as follows: Ferrostatin-1, 2 μΜ; Liproxstatin-1, 1 μM; Z-VAD-FMK, 10 μM; Necrosulfonamide, 0.5 μM; 3-Methyladenine, 250 μΜ; MG132, 10 μM; CHX 20 μM.
The Ethics Review Committee of Anhui Medical University has approved all animal experiments in accordance with the guidelines for the protection and use of experimental animals issued by the National Institute of Health Research.
Male C57BL/6 mice (20-28g, 6-9 weeks old) were purchased from Gempharmatech and housed in a pathogen-free, temperature-controlled environment (20℃-25℃) with a speci c facility providing a 12hour light-dark cycle. Each cage accommodated a maximum of 6 mice and they were fed a standard mouse diet.
The mice were randomly divided into four groups: Control group (intraperitoneal injection of sterile saline), LPS group (intraperitoneal injection of 10mg/kg LPS), LPS + ferrostatin-1 group (10mg/kg LPS + 1mg/kg ferrostatin-1), LPS + dapagli ozin group (dapagli ozin 10 mg/kg/day + intraperitoneal injection of 10mg/kg LPS). LPS(MCE) and ferrostatin-1(MCE) were administered via intraperitoneal injection, with LPS administered rst followed by ferrostatin-1. Dapagli ozin(MCE) was dissolved in sterile PBS and stored at -80℃. Dapagli ozin was administered via gastric lavage once daily for a consecutive period of ve days. On the 5th day, after oral gavage, intraperitoneal injection of LPS (10mg/kg) was performed, and 18 hours later, the mice were anesthetized with 50 mg/kg pentobarbital sodium. Blood samples were collected from the eyeballs. After anesthetizing the mice, the heart was perfused with PBS to obtain cardiac tissue.
Male C57BL/6 mice (20-28g, 6-9 weeks old) were purchased from Gempharmatech and housed in a pathogen-free, temperature-controlled environment (20℃-25℃) with a speci c facility providing a 12hour light-dark cycle. Each cage accommodated a maximum of 6 mice and they were fed a standard mouse diet.
The mice were randomly divided into four groups: Control group (intraperitoneal injection of sterile saline), LPS group (intraperitoneal injection of 10mg/kg LPS), LPS + ferrostatin-1 group (10mg/kg LPS + 1mg/kg ferrostatin-1), LPS + dapagli ozin group (dapagli ozin 10 mg/kg/day + intraperitoneal injection of 10mg/kg LPS). LPS(MCE) and ferrostatin-1(MCE) were administered via intraperitoneal injection, with LPS administered rst followed by ferrostatin-1. Dapagli ozin(MCE) was dissolved in sterile PBS and stored at -80℃. Dapagli ozin was administered via gastric lavage once daily for a consecutive period of ve days. On the 5th day, after oral gavage, intraperitoneal injection of LPS (10mg/kg) was performed, and 18 hours later, the mice were anesthetized with 50 mg/kg pentobarbital sodium. Blood samples were collected from the eyeballs. After anesthetizing the mice, the heart was perfused with PBS to obtain cardiac tissue.
Twenty‐four 6‐week‐old male ApoE−/− mice were purchased from the Model Animal Research Center of Nanjing University. The Institutional Animal Care and Utilization Committee of Fudan University Pudong Animal Experimental Center granted approval for all specific experimental procedures (2019‐M‐02). All the mice (six per group) were equally and randomly divided into the normal, HFD, HFD + ferrostatin‐1, and HFD + liproxstatin‐1 groups. The normal group was feed with normal diet, all other groups were fed with HFD (Research Diets, Inc. New Brunswick, NJ, USA) for 3 months. Accompanied with HFD, mice in ferrostatin‐1 group were injected (i.p.) with 1 mg/kg ferrostatin‐1 (diluted in 10% DMSO, 40% PEG300, 5% Tween‐80, and 45% saline) every 2 days. Mice in liproxstatin‐1 group were injected (i.p.) with 10 mg/kg liproxstain‐1 (diluted in similar solvent of ferrostatin‐1 group) every 2 days. Normal group and HFD group both received injection of blank solvent every 2 days.
After 3 months, all the mice were anaesthetized with 1% sodium pentobarbital and drawn blood from heart. Heart, liver, and aorta were harvested and fixed with 4% paraformaldehyde. Oil Red O staining for aorta and aortic sinus was performed as described above. Masson trichrome staining, TEM, IHC, and measurement of lipids were performed as described in previous research.
44 (link),
45 (link) NAS score was analyzed based on hematoxylin and eosin staining of liver. NAS include individual biopsy scores for steatosis (0−3), lobular inflammation (0−3), and hepatocellular ballooning (0−2) (TableS2 ).
46 (link) Serum ox‐LDL of mice was detected by commercial ELISA assay kit (Cat. CSB‐E07933m; CUSBIO, Wuhan, China). Methods for serum lipidome analysis and gut flora analysis were shown in supplementary files.
After 3 months, all the mice were anaesthetized with 1% sodium pentobarbital and drawn blood from heart. Heart, liver, and aorta were harvested and fixed with 4% paraformaldehyde. Oil Red O staining for aorta and aortic sinus was performed as described above. Masson trichrome staining, TEM, IHC, and measurement of lipids were performed as described in previous research.
44 (link),
45 (link) NAS score was analyzed based on hematoxylin and eosin staining of liver. NAS include individual biopsy scores for steatosis (0−3), lobular inflammation (0−3), and hepatocellular ballooning (0−2) (Table
46 (link) Serum ox‐LDL of mice was detected by commercial ELISA assay kit (Cat. CSB‐E07933m; CUSBIO, Wuhan, China). Methods for serum lipidome analysis and gut flora analysis were shown in supplementary files.
Top products related to «Ferrostatin-1»
Sourced in United States, Germany, United Kingdom, Italy, Japan
Ferrostatin-1 is a chemical compound used in research laboratories. It functions as a potent inhibitor of ferroptosis, a form of programmed cell death. Ferrostatin-1 is utilized in various experimental settings to study cellular mechanisms and pathways related to ferroptosis.
Sourced in United States, China
Ferrostatin-1 is a chemical compound used as a research tool in laboratory settings. It functions as a selective inhibitor of ferroptosis, a form of programmed cell death. The core purpose of Ferrostatin-1 is to serve as an experimental tool for investigating cellular processes and mechanisms related to ferroptosis.
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
Sourced in United States, China
Erastin is a chemical compound used as a research tool in laboratory settings. It functions as a small molecule inhibitor that induces ferroptosis, a form of regulated cell death. The core function of Erastin is to serve as a tool for studying cellular processes and potential therapeutic applications related to ferroptosis.
Sourced in United States, China
Ferrostatin-1 is a small molecule inhibitor that functions as a potent and selective inhibitor of ferroptosis, a form of regulated cell death driven by iron-dependent lipid peroxidation. It acts by blocking lipid peroxidation and ferroptosis without affecting other cell death pathways.
Sourced in United States, China, Germany, Switzerland, United Kingdom
Z-VAD-FMK is a broad-spectrum caspase inhibitor. It functions by irreversibly binding to the catalytic site of caspase enzymes, thereby blocking their activity.
Sourced in United States, Germany, Morocco, China
Erastin is a chemical compound used as a laboratory research tool. It functions as a ferroptosis inducer, capable of triggering a specific form of regulated cell death. The core function of Erastin is to disrupt cellular processes related to iron metabolism and lipid peroxidation. Detailed information about intended use or applications is not provided.
Sourced in United States, China
Erastin is a small molecule compound that functions as a potent and selective inhibitor of the system xc- cystine/glutamate antiporter. It is widely used in research applications to induce ferroptosis, a form of regulated cell death.
Sourced in United States, Germany
Ferrostatin-1 (Fer-1) is a small molecule that functions as a potent and specific inhibitor of ferroptosis, a form of regulated cell death. Ferroptosis is characterized by the iron-dependent accumulation of lethal lipid peroxides. Fer-1 acts by preventing this lipid peroxidation process, thereby inhibiting ferroptosis.
Sourced in United States, Germany, China, Canada, Italy, Sao Tome and Principe, United Kingdom, Japan, Macao, Australia
Z-VAD-FMK is a caspase inhibitor that functions as a broad-spectrum inhibitor of caspase enzymes. It is commonly used in research applications to study apoptosis and cell death processes.
More about "Ferrostatin-1"
Ferrostatin-1 (Fer-1) is a small molecule that inhibits ferroptosis, a form of programmed cell death involving iron-dependent lipid peroxidation.
This compound has been extensively studied for its potential therapeutic applications in various disease models, including neurodegenerative disorders, cancer, and ischemia-reperfusion injury.
Ferrostatin-1 has been shown to protect cells from oxidative stress by blocking the activation of the lipid peroxidation pathway.
Researchers can leverage PubCompare.ai's AI-driven platform to optimize their Ferrostatin-1 research.
This platform allows users to locate the best protocols from literature, pre-prints, and patents, and enhance the reproducibility and accuracy of their studies.
By utilizing this tool, researchers can streamline their research process and unlock new insights related to Ferrostatin-1 and its role in ferroptosis.
In addition to Ferrostatin-1, other key terms associated with this topic include fetal bovine serum (FBS), which is commonly used in cell culture experiments, Erastin, a compound that induces ferroptosis, and Z-VAD-FMK, a pan-caspase inhibitor that can be used in conjunction with Ferrostatin-1 to study the mechanisms of cell death.
By incorporating these related terms and concepts, researchers can gain a more comprehensive understanding of the role of Ferrostatin-1 in regulating cell fate and develop more effective strategies for targeting ferroptosis in various disease contexts.
This compound has been extensively studied for its potential therapeutic applications in various disease models, including neurodegenerative disorders, cancer, and ischemia-reperfusion injury.
Ferrostatin-1 has been shown to protect cells from oxidative stress by blocking the activation of the lipid peroxidation pathway.
Researchers can leverage PubCompare.ai's AI-driven platform to optimize their Ferrostatin-1 research.
This platform allows users to locate the best protocols from literature, pre-prints, and patents, and enhance the reproducibility and accuracy of their studies.
By utilizing this tool, researchers can streamline their research process and unlock new insights related to Ferrostatin-1 and its role in ferroptosis.
In addition to Ferrostatin-1, other key terms associated with this topic include fetal bovine serum (FBS), which is commonly used in cell culture experiments, Erastin, a compound that induces ferroptosis, and Z-VAD-FMK, a pan-caspase inhibitor that can be used in conjunction with Ferrostatin-1 to study the mechanisms of cell death.
By incorporating these related terms and concepts, researchers can gain a more comprehensive understanding of the role of Ferrostatin-1 in regulating cell fate and develop more effective strategies for targeting ferroptosis in various disease contexts.