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Fluoroshield

Fluoroshield: A revolutionary AI-driven platform that empowers researchers to optimize their research protocols and enhance reproducibility.
Leveraging powerful search and comparison tools, Fluoroshield helps locate the most effective and reliable protocols from literature, preprints, and patents.
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Most cited protocols related to «Fluoroshield»

1
Staining Collagen-Hybridizing Peptides on Tissue Sections
Cited 7 times
For OCT-embedded tissue sections, the
OCT compound was removed by rinsing with PBS for 5 min after tissue
slides were warmed to room temperature. For FFPE tissue sections,
paraffin was removed by rinsing with xylene, 100% ethanol, 95% ethanol,
50% ethanol, and deionized water for two 5 min cycles of each solvent
in consecutive order. The deparaffinized tissue was directly used
for staining without any antigen-retrieval process. After the embedding
material was removed, 5% goat serum in PBS was added to the tissue
sections and incubated for 20 min at room temperature to block nonspecific
binding. For B-CHP staining, any endogenous biotin in the tissue was
blocked using the endogenous biotin-blocking kit following the manufacturer’s
protocol after the serum blocking step. Because CHP can self-assemble
into homotrimers in solution over time (e.g., during storage at 4 °C) and lose its driving force
for collagen hybridization, the trimeric CHP was thermally dissociated
to single strands by heating briefly at 80 °C before it was used
to bind unfolded collagen.25 (link) To prevent
undesired thermal denaturation of the tissue, the 80 °C CHP solution
was quenched quickly to room temperature immediately prior to addition
to tissue samples (dead time <2 min). The CHP trimerization has
a third-order folding rate with a half-time on the order of hours
at low μM concentrations.26 ,65 (link),66 (link) Therefore, the heating and quenching protocol used here (Figure S1) ensures that CHP strands predominate
in the active monomer form until exposed to denatured collagen. After
the blocking solutions were gently removed from the slides, heat-activated
solutions of single-strand CHPs were added to the tissue sections
in the following fashion. CHP solution (15 μM of B-CHP or F-CHP,
50–200 μL per section) in PBS or PBS containing 1% BSA
(when costaining with an antibody) was heated for 5 min in an 80 °C
water bath to dissociate the trimeric peptides, followed by immediate
incubation in an ice/water bath (for 15–90 s depending on the
solution volume) to quench the hot solution to room temperature. When
needed, an antibody was diluted into this quenched CHP solution for
costaining. The solution containing the quenched CHP monomers was
quickly pipetted to each slide (usually 50–200 μL per
section) within 1 min. The tissue samples were incubated in a humidity
chamber at 4 °C overnight. After staining, the slides were incubated
for 5 min in 100 mL of PBS at room temperature to remove the unbound
material and the washing step was repeated three times. For B-CHP
staining, the tissue sections were subsequently incubated with AlexaFluor647-labeled
streptavidin (0.005 mg/mL) in a PBS solution containing 1% BSA. To
detect the costained primary antibody, a labeled secondary antibody
was either diluted into the AlexaFluor647-streptavidin solution (for
B-CHP costaining) or added to the slides directly after dilution in
a PBS solution containing 1% BSA (for F-CHP costaining). The tissues
were incubated with the streptavidin and/or secondary antibody solution
in a humidity chamber for 1 h at room temperature. Where indicated,
cell nuclei were stained with Hoechst 33342 in PBS for 20 min according
to the manufacturer’s recommendation. Finally, the tissue sections
were rinsed with PBS three times and mounted with Fluoroshield. The
specific CHP, antibody types, and concentrations used in each experiment
are presented in Table S1. All histological
staining experiments were performed on at least three different sections
from each tissue sample. For the myocardial infarction, glomerulonephritis,
lung fibrosis, and skin aging experiments, tissue samples from three
animals within each experimental group (e.g., the normal control group, the week 3 group) were analyzed.
Hwang J., Huang Y., Burwell T.J., Peterson N.C., Connor J., Weiss S.J., Yu S.M, & Li Y. (2017). In Situ Imaging of Tissue Remodeling with Collagen Hybridizing Peptides. ACS Nano, 11(10), 9825-9835.
Publication 2017
Antigens Bath Biotin Cardiac Arrest Cell Nucleus Collagen Crossbreeding Ethanol Fluoroshield Glomerulonephritis Goat HOE 33342 Hot Temperature Humidity Immunoglobulins Myocardial Infarction Paraffin Peptides Pulmonary Fibrosis Serum Streptavidin Technique, Dilution Tissues Xylene
2
Histological and Immunohistochemical Analysis of Avian Retina
Cited 7 times
Eyes were hemisected through the equator, vitreous was removed, and posterior eye cups were fixed in 4% paraformaldehyde + 3% sucrose in 0.1 M phosphate buffer, pH 7.4, for 0.5 hrs (25 °C), then washed three times (15 min each) in PBS, and cryoprotected in 0.1 M phosphate buffer + 30% sucrose for 2–4 days at 4 °C. For cryosectioning, eye cups were soaked in OCT (VWR) for 15 min (25 °C) and quick-frozen using dry ice/ethanol. Sections were cut at 12–14 µm, thaw-mounted onto Fisherbrand Superfrost Plus slides (ThermoFischer Scientific), briefly heat-fixed on a hot plate (40 °C, 10 min), ringed with rubber cement to create a hydrophobic barrier, and stored at −20 °C.
For histology, slides were warmed (25 °C), washed three times (15 min) in PBS, and incubated under 0.1% (w/v) Toluidine blue58 (link). After 2 min, the stain was removed, and the slides were washed in PBS and mounted under cover-slips in a 4:1 solution of glycerol:water. For detecting signs of damage by immunolabelling, we used two monoclonal mouse antibodies (both known to be specific for application to chicken tissues) from the Developmental Studies Hybridoma Bank (DSHB; University of Iowa, Ames, IA, USA): anti-LEP-100 (lysosomal membrane glycoprotein, cv24)59 (link) and anti-GRL2 (activated leukocyte cell-surface glycoprotein GRL2)29 (link), which are markers for microglia/macrophages26 (link), 60 (link) and activated phagocytes/granulocytes61 (link), respectively. Sections were warmed, washed, and then incubated overnight at 25 °C in LEP-100 (1:50) or GRL2 (1:500) antibodies, diluted in PBS + 0.025% Triton X-100. After incubation, the slides were washed in PBS and then incubated under 1:1000 AF488 donkey anti-mouse secondary antibody (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) for 2 hrs. Slides were washed again in PBS, and mounted using Fluoroshield mounting medium + DAPI (Abcam Inc., Toronto, ON, Canada). Toluidine blue-labeled slides were imaged using a Zeiss epi-illumination microscope with 25x Neo-Fluar water-immersion objective, NA = 0.8, and digital camera (Model RT3; SPOT Imaging, Division of Diagnostic Instruments, Inc., Sterling Heights, Michigan, USA). LEP-100- and GRL2-labeled slides were imaged using a laser-scanning confocal microscope with 40x oil-immersion objective, NA = 1.3 (Model FV1000; Olympus Corporation of the Americas, Center Valley, PA, USA). Image post-processing was performed using Adobe Photoshop CS5 (Adobe Systems Incorporated, San Jose, CA, USA).
Carr B.J, & Stell W.K. (2016). Nitric Oxide (NO) Mediates the Inhibition of Form-Deprivation Myopia by Atropine in Chicks. Scientific Reports, 6, 9.
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Publication 2016
Antibodies Antibodies, Anti-Idiotypic Buffers Chickens DAPI Dental Cementum Dry Ice Equus asinus Ethanol Fingers Fluoroshield Freezing Glycerin Hybridomas Leukocytes Light Microscopy Lysosomal Membrane Glycoproteins Membrane Glycoproteins Microglia Microscopy, Confocal Mineralocorticoid Excess Syndrome, Apparent Monoclonal Antibodies Mus paraform Phagocytes Phosphates Rubber Stains Submersion Sucrose Tissues Tolonium Chloride Triton X-100
3
DNA Repair Assay in OPSCC Cells
Cited 6 times
OPSCC cells were grown on 13 mm coverslips until ~70–80 % confluent, irradiated at 4 Gy and incubated for the required time in 5 % CO2 at 37°C to allow for DNA repair. Cells were washed with PBS at room temperature for 5 min before being fixed using 4 % paraformaldehyde for 10 min. Cells were permeabilised with 0.2 % Triton X-100 in PBS for 10 min, then washed three times with 0.1 % Tween-20 for 10 min. Coverslips were blocked to avoid non-specific staining via incubation with 2 % BSA for 30 min at room temperature on a rocking platform with either γH2AX, 53BP1 or RAD51 antibodies in 2 % BSA overnight at 4°C. Following three washes with PBS, coverslips were incubated with either goat anti-mouse Alexa Fluor 555 or goat anti-rabbit Alexa Fluor 488 secondary antibodies in 2% BSA for 1 h at room temperature in the dark. Finally samples were washed with PBS for 10 min on a rocking platform and mounted on a microscope slide using Fluoroshield containing DAPI (Sigma-Aldrich, Gillingham, UK). Cells were examined using an Olympus BX61 fluorescent microscope with a Photometrics CoolSNAP HQ2 CCD camera. MicroManager software was used to capture images (~500 images/cell line/antibody).
Nickson C.M., Moori P., Carter R.J., Rubbi C.P, & Parsons J.L. (2017). Misregulation of DNA damage repair pathways in HPV-positive head and neck squamous cell carcinoma contributes to cellular radiosensitivity. Oncotarget, 8(18), 29963-29975.
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Publication 2017
alexa fluor 488 Alexa Fluor 555 Anti-Antibodies Antibodies Cell Lines Cells DAPI DNA Repair Fluoroshield Goat Immunoglobulins Microscopy Mus paraform Rabbits TP53BP1 protein, human Triton X-100 Tween 20
4
Drosophila Thorax Cryosectioning and Staining
Cited 4 times
Head, wings and abdomen were removed from one day old w1118 flies and thoraxes were fixed overnight at 4°C in 4% PFA. For 30–90 hr APF samples, pupae were freed from the pupal case, poked 3–5 times with an insect pin in the abdomen and fixed overnight at 4°C in 4% PFA. Thoraxes or pupae were then sunk in 30% sucrose in 0.5% PBS-T overnight at 4°C on a nutator. Thoraxes or pupae were embedded in Tissue-Tek O.C.T. (Sakura Finetek) in plastic moulds (#4566, Sakura Finetek) and frozen on dry ice. Blocks were sectioned at 30 µm on a cryostat (Microm vacutome). Sections were collected on glass slides coated with 1% gelatin +0.44 μM chromium potassium sulfate dodecahydrate to facilitate tissue adherence. Slides were post-fixed for 1 min. in 4% PFA in 0.5% PBS-T at RT, washed in 0.5% PBS-T, incubated with rhodamine-phalloidin for 2 hr at RT, washed three times in 0.5% PBS-T and mounted in Fluoroshield with DAPI (#F6057, Sigma).
Spletter M.L., Barz C., Yeroslaviz A., Zhang X., Lemke S.B., Bonnard A., Brunner E., Cardone G., Basler K., Habermann B.H, & Schnorrer F. (2018). A transcriptomics resource reveals a transcriptional transition during ordered sarcomere morphogenesis in flight muscle. eLife, 7, e34058.
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Publication 2018
Abdominal Cavity Chest Chromium chromium sulfate DAPI Diptera Dry Ice Fluoroshield Freezing Fungus, Filamentous Gelatins Head Insecta Potassium potassium sulfate Pupa rhodamine-phalloidin Sucrose Tissues
5
EV71 Capsid Protein VP-1 Antibody Protocol
Cited 4 times
Mouse monoclonal antibody against EV71 capsid protein VP-1 was purchased from Abcam (Cambridge, UK). Rabbit monoclonal antibody to AP2M1 (Abcam), Rabbit polyclonal antibodies to FCHO2 (ProSci, Poway, CA, USA), eIF4G (Cell Signaling Technology, Danvers, MA, USA) and Enterovirus 71 2C and 3C (GeneTex, Irvine, CA, USA), Mouse monoclonal antibodies against clathrin (CLTC, Santa Cruz Biotechnology, Dallas, Texas, USA) and dynamin (Santa Cruz Biotechnology) were used in Western-blot detection of the respective proteins. The secondary antibody conjugated to Allophycocyanin (APC) was from BD Biosciences (Franklin Lakes, CA, USA). AlexaFluor594-Conjugated AffiniPure Goat Anti-Mouse IgG (H + L) was from ZSGB-BIO (Beijing, China). Fluoroshield Mounting Medium with DAPI was from Abcam. AlexaFluor 488 conjugated transferrin from human serum and AlexaFluor 594 conjugated cholera toxin subunit B were from ThermoFisher scientific (Waltham, MA, USA). Chloropromazin and dynasore were purchased from Selleckchem (Shanghai, China). Guanidine hydrochloride (GnHCl) was from MedChem Express (Monmouth Junction, NJ, USA). All drugs were prepared according to the manufacturer’s instructions under sterile conditions.
Yuan M., Yan J., Xun J., Chen C., Zhang Y., Wang M., Chu W., Song Z., Hu Y., Zhang S, & Zhang X. (2018). Enhanced human enterovirus 71 infection by endocytosis inhibitors reveals multiple entry pathways by enterovirus causing hand-foot-and-mouth diseases. Virology Journal, 15, 1.
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Publication 2018
allophycocyanin anti-IgG Antibodies Capsid Proteins Choleragenoid Clathrin DAPI Dynamins Enterovirus Eukaryotic Initiation Factor-4G Fluoroshield Goat Homo sapiens Hydrochloride, Guanidine Immunoglobulins Monoclonal Antibodies Mus N'-(3,4-dihydroxybenzylidene)-3-hydroxy-2-naphthahydrazide Pharmaceutical Preparations Proteins Rabbits Serum Sterility, Reproductive Transferrin Western Blotting

Most recents protocols related to «Fluoroshield»

1
Evaluating BM-MSC Polarization and Morphology
For the evaluation of BM-MSC polarization and the cellular morphology, upon TP treatment, the cells were seeded in 8-well chamber slides (SPL Life Sciences, #30108) at a density of 2x104 cells per well and incubated overnight to allow attachment, followed by treatment with 10-8 M TP for 24 hours. The slides were fixed and permeabilized as previously described23 and labeled either with anti-CXCL9 PE (Clone: J1015E10, Biolegend, #357903) and anti-CXCL5 APC (Clone: J111B7, Biolegend, #524105) antibodies or with F-actin probe Phalloidin (Alexa Fluor 555 conjugated, Thermo Fisher Scientific, #A34055) by incubating overnight at 4 °C. Cell nuclei were counterstained with DAPI, and the slides were mounted with FluoroShield medium (Sigma Aldrich, #F6182). Micrographs were taken with a Zeiss LSM 780 confocal microscope.
Aru B., Akdeniz T., Dağdeviren H., Gürel G, & Yanıkkaya Demirel G. (2023). Testosterone Propionate Promotes Proliferation and Viability of Bone Marrow Mesenchymal Stem Cells while Preserving Their Characteristics and Inducing Their Anti-Cancer Efficacy. Balkan Medical Journal, 40(2), 117-123.
Publication 2023
Alexa Fluor 555 Antibodies Cell Nucleus Cells Clone Cells CXCL5 protein, human CXCL9 protein, human DAPI F-Actin Fluoroshield Microscopy, Confocal Phalloidine
2
Microglia and Autofluorescence Analysis of Human Brain Tissue
Human brain tissue for this study was obtained from the Department of Pathology and Laboratory Medicine at UT Health Science Center at Houston. Blocks of tissue were dissected from the brains of three deceased individuals (14-year-old female, 75-year-old male, and 97-year-old female). To be consistent with the animal study, the somatosensory and visual cortex were chosen to perform the microglia and autofluorescence analysis. Formalin-fixed paraffin-embedded sections were immunostained with Iba1 antibody using tyramide signal amplification method (Biotium). Briefly, after deparaffinization, the sections were blocked in 0.3% hydrogen peroxide and blocking buffer (1% bovine serum albumin with 0.5% Triton X-100 in PBS), serially, and then the sections were incubated with anti-human Iba1 mouse monoclonal antibody (1:200; FUJIFILM, catalog no. NCNP27). The primary antibody was detected by horseradish peroxidase–conjugated goat anti-mouse secondary antibody (1:200) and colorized with CF488A tyramide dye in amplification buffer. Without autofluorescence elimination, the sections were coverslipped using Fluoroshield with DAPI mounting solution (Sigma-Aldrich). To obtain the representative images, the neocortex and subcortical white matter was scanned using a Leica THUNDER Imager DMi8 under 10× lens, and the high-magnification images (63× lens) were also taken from the same regions.
Ritzel R.M., Li Y., Jiao Y., Lei Z., Doran S.J., He J., Shahror R.A., Henry R.J., Khan R., Tan C., Liu S., Stoica B.A., Faden A.I., Szeto G., Loane D.J, & Wu J. (2023). Brain injury accelerates the onset of a reversible age-related microglial phenotype associated with inflammatory neurodegeneration. Science Advances, 9(10), eadd1101.
Publication 2023
Animals Antibodies, Anti-Idiotypic Brain Buffers DAPI Females Fluoroshield Formalin Goat Homo sapiens Horseradish Peroxidase Immunoglobulins Lens, Crystalline Males Mice, House Microglia Monoclonal Antibodies Neocortex Paraffin Peroxides Serum Albumin, Bovine Tissues Tritium Triton X-100 Visual Cortex White Matter
3
Multicolor Immunofluorescence Staining for CD68, S100A4, SPARC, and SPP1
For IF double staining mouse anti-CD68 monoclonal antibody (1:100, #NBP2-44539, clone KP1, Novus Biologicals); polyclonal rabbit anti-S100A4 (1:1000, PA5-82322, Thermo Fisher Scientific, USA), polyclonal goat anti-SPARC (1:80, AF941, R&D Systems, USA) and polyclonal goat anti-SPP1 (1:80, AF1433, R&D Systems, USA) were used. Combination of secondary antibodies were applied: donkey Cy3-conjugated anti-rabbit antibody (#711-165-152, Dianova, Germany, dilution 1:400), donkey AlexaFluor488-conjugated anti-mouse antibody (#715-545-150, Dianova, Germany, dilution 1:400) and donkey Cy3-conjugated anti-goat antibody (#706-167-003, Dianova, Germany, dilution 1:400). Samples were mounted with Fluoroshield Mounting Medium with DAPI (#ab104135, Abcam, USA) and analyzed by confocal microscopy. Confocal laser scanning microscopy was performed with Carl Zeiss LSM 780 NLO laser scanning spectral confocal microscope (Carl Zeiss, Germany), equipped with 40x objective. Data were acquired and analyzed with Black Zen software (RRID : SCR_018163). All three-color images were acquired using a sequential scan mode.
Kazakova E., Rakina M., Sudarskikh T., Iamshchikov P., Tarasova A., Tashireva L., Afanasiev S., Dobrodeev A., Zhuikova L., Cherdyntseva N., Kzhyshkowska J, & Larionova I. (2023). Angiogenesis regulators S100A4, SPARC and SPP1 correlate with macrophage infiltration and are prognostic biomarkers in colon and rectal cancers. Frontiers in Oncology, 13, 1058337.
Full text: Click here
Publication 2023
Antibodies Antibodies, Anti-Idiotypic Biological Factors Clone Cells DAPI Equus asinus Fluoroshield Goat Microscopy, Confocal Mus Novus Rabbits Radionuclide Imaging SPARC protein, human SPP1 protein, human Technique, Dilution
4
Cartilage Extracellular Matrix Characterization
Cartilage discs were embedded in Frozen Section Medium (Richard-Allan Scientific Neg50, Thermo Scientific) and frozen in dry ice-cooled isopentane. Frozen tissue blocks were stored at −80°C. The samples were cut in 9–10 µm thick sections on a CryoStar™ NX70 Cryostat (Thermo Scientific), mounted on SuperFrost Plus Adhesion slides and stored at −80°C. Sections were immediately post-fixed for 60 s in cold 95% ethanol directly before immunostaining. Sections were immunostained for the presence of ACAN (clone 4F4; Santa Cruz; at 0.1 µg/mL), COL2 (clone II-II6B3-a; DSHB; at 2.45 µg/mL), COL1 (clone EPR7785; Abcam; at 0.8 µg/mL), COL10 (clone X53 diluted 1:200; generous gift from Prof. Klaus von der Mark). Slides were incubated with primary antibodies diluted in 1.25% BSA in PBS at 4°C overnight. Negative controls were made by omitting primary antibodies (Supplementary Figure S5). The secondary antibodies, goat anti-rabbit IgG conjugated to Alexa 488 and goat anti-mouse IgG conjugated to Alexa 594 (Life Technologies), were diluted 1:400. The stained sections were mounted with Fluoroshield (Sigma), containing DAPI for nuclear staining. Samples were analyzed using an upright Nikon Eclipse E600 microscope equipped with an Olympus ColorView III camera. Images were opened in the image processing software Fiji (Schindelin et al., 2012 (link)), colour (red, green, blue) was assigned to the images, and images were merged. Thickness of cartilage discs was measured on the stained sections using Fiji. Five measures from representative areas of a section from each donor were taken. Nuclei were counted on the basis of DAPI stained sections prepared for immunofluorescence analysis. 20 equally-sized fields (200 × 200 px) were counted per donor using Fiji. Sections were prepared from 1 disc per donor.
Frerker N., Karlsen T.A., Stensland M., Nyman T.A., Rayner S, & Brinchmann J.E. (2023). Comparison between articular chondrocytes and mesenchymal stromal cells for the production of articular cartilage implants. Frontiers in Bioengineering and Biotechnology, 11, 1116513.
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Publication 2023
Alexa594 anti-IgG Antibodies Cartilage Cell Nucleus Clone Cells Cold Temperature Cryoultramicrotomy DAPI Dry Ice E-600 Ethanol Fluorescent Antibody Technique Fluoroshield Freezing Goat isopentane Mice, House Microscopy Rabbits Tissue Donors Tissues
5
Immunofluorescence Analysis of Corneal Fibroblasts
The F1 RCFs were incubated with primary antibodies against Vimentin (1:200, ab45939), Lumican (1:100, bs-5890R), and Keratocan (1:200, bs-11054R-PE) at 4°C overnight. Immunoreactivity was evaluated using two fluorescein isothiocyanate (FITC)-labeled secondary antibodies (Abcam Inc., Cambridge, MA, USA) and a fluorescent secondary antibody (Cy3; Bioss, Beijing, China), and cell nuclei were counterstained with Fluoroshield containing 4′-6-diamidino-2-phenylindole (DAPI; Sigma F6057-20ML). Preparations were evaluated using fluorescence microscopy (Nikon 80i). RCFs were identified by flow cytometry to detect Vimentin according to the manufacturer's protocol.
Liu R., Li J., Guo Z., Chu D., Li C., Shi L., Zhang J., Zhu L, & Li Z. (2023). Celastrol Alleviates Corneal Stromal Fibrosis by Inhibiting TGF-β1/Smad2/3-YAP/TAZ Signaling After Descemet Stripping Endothelial Keratoplasty. Investigative Ophthalmology & Visual Science, 64(3), 9.
Publication 2023
Antibodies Cell Nucleus DAPI Flow Cytometry Fluorescein Fluorescent Antibody Technique Fluoroshield isothiocyanate LUM protein, human Microscopy, Fluorescence Vimentin

Top products related to «Fluoroshield»

1
Fluoroshield with dapi by Merck Group
Sourced in United States, Germany, Spain, Italy, United Kingdom, Japan
Fluoroshield with DAPI is a fluorescent mounting medium designed for use with DNA-binding fluorescent dyes. It provides a protective environment for the fluorescent signal and helps preserve the integrity of the sample.
2
Fluoroshield by Merck Group
Sourced in United States, Germany, United Kingdom, France, Canada, Hungary, Japan, Spain
Fluoroshield is a protective screen for use in laboratory settings. It is designed to shield against the potential hazards of fluorescent light exposure. The core function of Fluoroshield is to provide a barrier that safeguards personnel in the vicinity from excessive ultraviolet (UV) and visible light emissions.
3
Fluoroshield mounting medium with dapi by Abcam
Sourced in United Kingdom, United States
Fluoroshield Mounting Medium with DAPI is a ready-to-use aqueous mounting medium formulated for the preservation and visualization of fluorescently labeled samples. It contains the nucleic acid stain DAPI, which binds to DNA and emits blue fluorescence when excited.
4
Ab104139 by Abcam
Sourced in United Kingdom, United States, China, Germany, Japan
Ab104139 is a primary antibody product offered by Abcam. It is intended for use in various research applications. The core function of this product is to bind to a specific target, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
5
Fluoroshield mounting medium by Abcam
Sourced in United Kingdom, United States
Fluoroshield Mounting Medium is a glycerol-based mounting medium designed for preserving and protecting fluorescence signals in fixed and immunostained samples. It helps maintain the integrity and brightness of fluorescent dyes and proteins.
6
Alexa fluor 488 by Thermo Fisher Scientific
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Alexa Fluor 488 is a fluorescent dye used in various biotechnological applications. It has an excitation maximum at 495 nm and an emission maximum at 519 nm, producing a green fluorescent signal. Alexa Fluor 488 is known for its brightness, photostability, and pH-insensitivity, making it a popular choice for labeling biomolecules in biological research.
7
Fluoroshield mounting medium by Merck Group
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Fluoroshield mounting medium is a laboratory product designed for preserving fluorescent signals in microscopy samples. It is a ready-to-use solution that helps maintain the brightness and stability of fluorophores when applied to prepared slides.
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DAPI is a fluorescent dye that binds strongly to adenine-thymine (A-T) rich regions in DNA. It is commonly used as a nuclear counterstain in fluorescence microscopy to visualize and locate cell nuclei.
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Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
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Triton X-100 is a non-ionic surfactant commonly used in various laboratory applications. It functions as a detergent and solubilizing agent, facilitating the solubilization and extraction of proteins and other biomolecules from biological samples.

More about "Fluoroshield"

Fluoroshield is a revolutionary AI-driven platform that empowers researchers to optimize their research protocols and enhance reproducibility.
Leveraging powerful search and comparison tools, Fluoroshield helps locate the most effective and reliable protocols from literature, preprints, and patents.
Its AI-driven analysis ensures researchers can advance their work with confidence, experiencing the future of protocol optimization.
The platform's powerful features include the ability to search and compare a wide range of research protocols, including those related to techniques like immunofluorescence, flow cytometry, and microscopy.
Fluoroshield's AI-driven analysis can identify the most effective and reliable protocols, taking into account factors such as experimental conditions, reagents, and reported outcomes.
Fluoroshield also offers integration with other tools and resources, such as Fluoroshield with DAPI, Fluoroshield Mounting Medium, and Alexa Fluor 488, to provide a comprehensive solution for researchers.
The platform's user-friendly interface and advanced features make it easy for researchers to navigate and optimize their workflows, ensuring they can achieve consistent and reproducible results.
Whether you're working with DAPI, Bovine serum albumin, Triton X-100, or any other research-related reagents and techniques, Fluoroshield can help you streamline your processes and unlock new insights.
Experience the future of protocol optimization with Fluoroshield today.