Fluoxetine Hydrochloride

All reagents used in the experiments were analytical grade and used as received without further purification. Bupropion hydrochloride (BUP) and mirtazapine (MTZ) were obtained from LGC (Wesel, Germany). Amisulpride (AMS), amitriptyline (AMT), fluoxetine hydrochloride (FLX), olanzapine (OLN), paroxetine (PRX) and venlafaxine hydrochloride (VNX) were purchased from TCI (Zwijndrecht, Belgium). Bezafibrate (BZF), budesonide (BUD), caffeine (CAF), carbamazepine (CBZ), citalopram (CIT), clofibric acid (CA), clozapine (CZP), diclofenac (DCF), fenofibrate (FNB), fluvoxamine (FXM), gemfibrozil (GMF), haloperidole (HAL), norfluoxetine hydrochloride (NFX), O-desmethyl venlafaxine (ODV), quetiapine (QTP), risperidone (RIS), salicylic acid (SA), sertraline hydrochloride (STR), simvastatin (SIM), sulfadiazine (SDZ), sulfamethoxazole (SMX), sulfapyridine (SPY), sulfathiazole (STZ), tolfenamic acid (TA) and trimethoprim (vetranal) (TMP) were purchased from Sigma-Aldrich (Darmstadt, Germany). N-desmethyl olanzapine (DMO) and N-desmethyl sertraline hydrochloride (DMS) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The structures and main physicochemical properties of pharamceutical compounds are presented in Table S1. Amitriptyline-d6 hydrochloride, carbamazepine-d₁₀, fluoxetine-d₅ hydrochloride, haloperidol-d₄ and olanzapine-d₃ were supplied by A2S (Saint Jean d’Illac, France). Individual stock solutions of each compound, as well as isotopically labeled internal standard solutions, were prepared in methanol and stored at −20 °C. LC-MS grade methanol and water, and Na₂EDTA were purchased from Fisher Scientific (Leicestershire, UK). Folin–Ciocalteu’s phenol reagent, Bis-Tris and formic acid (purity: 98–100%) were obtained from Merck KGaA (Darmstadt, Germany). Sodium carbonate and p-hydroxy benzoic acid were supplied by Sigma Aldrich (St. Louis, MO, USA). Boric acid was obtained from Supelco (Bellefonte, PA, USA). Oasis HLB (200 mg, 6 cm³) cartridges used for solid phase extraction were bought from Waters Corporation (Milford, MA, U.S.A.). Graphitic carbon nitride (g-C₃N₄) (Specific Surface Area: 35 m²g⁻¹, particle size of 25 nm, E_g = 2.82 eV) synthesis and characterization used as photocatalyst are described in a previous publication from our group [9 (link)].

Fluoxetine hydrochloride (Prozac^®, FLX) was purchased from Eli Lilly and Company Ltd. (Basingstoke, England). Test samples were freshly prepared every day in saline (NaCl, 0.9%) and administered p.o. in a volume of 10 mL kg^-1 at the doses stated. Vehicle or control animals received 0.9% saline in a volume of 10 mL kg^-1. Administration of test compounds was done via an oral gavage.