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Formic acid, ammonium salt

Formic acid, ammonium salt is a chemical compound with the molecular formula CH3NO2.
It is the ammonium salt of formic acid and is commonly used in various industrial and research applications.
This compoun can be utilized as a preservative, antimicrobial agent, and pH adjuster.
Researchers may find it useful in a variety of experiemental protocols, such as those involving organic synthesis, biochemical assays, and material science.
The PubCompare.ai platform can help locate optimal research protocols and procedures for working with formic acid, ammonium salt, enhancing reproducibility and optimizing experiements.

Most cited protocols related to «Formic acid, ammonium salt»

Typically, 0.65 g of lyophilized cell walls was used for one experiment. Proteins were extracted by successive salt solutions in this order: two extractions each time with 6 mL CaCl2 solution (5 mM acetate buffer, pH 4.6, 0.2 M CaCl2 and 10 μL protease inhibitor cocktail), followed by two extractions with 6 mL LiCl solution (5 mM acetate buffer, pH 4.6, 2 M LiCl and 10 μL protease inhibitor cocktail). Cell walls were resuspended by vortexing for 5–10 min at room temperature, and then centrifuged for 15 min at 4000 × g and 4°C. Supernatants were desalted using Econo-Pac® 10 DG columns (Bio-Rad) equilibrated with 0.2 formic acid ammonium salt. The extract were lyophilized and resuspended in sample buffer for separation of proteins by 1D-GE, as previously described [12 (link)].
The next extraction was carried out by SDS and DTT. The cell wall preparation was treated with 12 mL solution containing 62.5 mM Tris, 4% SDS, 50 mM DTT, pH 6.8 (HCl). The mixture was boiled for 5 min and centrifuged for 15 min at 40000 × g and 4°C. The supernatant was dialyzed against 1 L H2O in Spectra/Por® membrane 10 kDa MWCO bags (Spectrum Medical Industries) at room temperature, then concentrated by successive centrifugation using the Centriprep® centrifugal filter devices (YM-10 kDa membrane) (Millipore) at 4000 × g followed by speed vacuum centrifugation.
The protein content of each extract was measured using the Bradford method [27 (link)] with the Coomassie™ protein assay reagent kit (Pierce) using bovine serum albumin (BSA) as standard.
Gels were stained with Coomassie™ Brilliant Blue-based method [28 (link)]. Colored bands were digested with trypsin and MALDI-TOF MS or LC-MS/MS analyses were performed as previously reported [12 (link),13 (link)].
The sequences of the identified proteins were subsequently analyzed with several bioinformatic programs to predict their sub-cellular localization [29 -31 ]. In some cases, predictions were not the same with the three programs. Results are then indicated as "not clear". Data are described in Tables 1–5 (additional data).
Publication 2006
Acetate Amino Acid Sequence Biological Assay brilliant blue G Buffers Cells Cell Wall Centrifugation formic acid, ammonium salt Gastrin-Secreting Cells Gels Medical Devices Protease Inhibitors Proteins Serum Albumin, Bovine Sodium Chloride Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Staining Tandem Mass Spectrometry Tissue, Membrane Tromethamine Trypsin Vacuum
The reversed phase chromatographic conditions were adapted from Hu et al. (2008 (link)). Methanol was preferred to acetonitrile since it has been observed that it decreased the carryover of most apolar lipid species (data not shown). CSF total lipid extracts were separated on an Dionex Ultimate 3000 UPLC system (Thermo Scientific, San Jose, CA) using a kinetex C8 150 × 2.1 mm, 2.6 μm column (Phenomenex, Sydney, NSW, Australia). Mobile phase A consisted of H2O/MeOH 60/40 (v/v) and 0.1 % formic acid and mobile phase B of IPA/MeOH 90/10 (v/v) and 0.1 % formic acid. Ammonium formate (10 mM) was added to both mobile phases in the positive ion mode. The presence of ammonium salt in mobile phases decrease drastically the detection of sodium adducts which now constitute minor lipid species in the positive ion mode (data not shown). The gradient program was as follows: solvent B was maintained for 2.5 min at 32 %, from 2.5 to 3.5 min it was increased to 45 % B, from 3.5 to 5 min to 52 % B, from 5 to 7 min to 58 % B, from 7 to 10 min to 66 % B, from 10 to 12 min to 70 % B, from 12 to 15 min to 75 % B, from 15 to 19 min to 80 % B, from 19 to 22 min to 85 % B, and from 22 to 23 min to 95 % B; from 23 to 25 min, 95 % B was maintained; from 25 to 26 min solvent B was decreased to 32 % and then maintained for 4 min for column re-equilibration. The flow rate was 400 μL/min and the column temperature was set to 60 °C.
Publication 2016
acetonitrile Chloride, Ammonium Chromatography, Reverse-Phase formic acid formic acid, ammonium salt Lipids Methanol Sodium Solvents
Metabolites were measured in 1,768 subjects from the KORA F4 study by Metabolon, Inc. (Durham, NC, USA), a commercial supplier of metabolic analyses, who has developed a platform that integrates the chemical analysis, including identification and relative quantification, data-reduction and quality-assurance using three separate analytical methods (GC–MS, LC–MS (positive mode), LC–MS (negative mode)) to detect as wide a range of metabolites as possible (Evans et al. 2009 (link); Suhre et al. 2011 (link)).
Sample preparation was assisted by a Hamilton ML STAR (Hamilton Company, Salt Lake City, UT, USA) robotics system. After thawing, 400 μl of extraction solvent (i.e. methanol, containing recovery standards) was added to each 100 μl of serum samples in a 96 deep well plate format. Extraction was carried out by shaking for 2 min using a Geno/Grinder 2000 (Glen Mills Inc., Clifton, NJ, USA). After centrifugation the supernatant was split into four aliquots: two for LC/MS analysis (positive and negative electrospray ionization mode), one for GC/MS analysis and one reserve aliquot. Solvent was removed on a TurboVap (Zymark) and the samples were dried under vacuum overnight. For LC/MS pos. ion mode samples were reconstituted with 0.1 % formic acid, for neg. ion mode with 6.5 mM ammonium bicarbonate pH 8.0. Both reconstitution solvents contained also internal standards. The GC/MS aliquots were derivatized for 1 h at 60 °C with N,O-bistrimethylsilyl-trifluoroacetamide in a solvent mixture of acetonitrile:dichlormethane:cyclohexane (5:4:1), containing 5 % triethylamine and retention time markers.
LC/MS analysis was performed on a LTQ mass spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA) equipped with a Waters Acquity UPLC system (Waters Corporation, Milford, MA, USA). Two separate columns (2.1 × 100 mm Waters BEH C18 1.7 μm particle) were used for acidic (solvent A: 0.1 % formic acid in H2O, solvent B: 0.1 % formic acid in methanol) and basic (A: 6.5 mM ammonium bicarbonate pH 8.0, B: 6.5 mM ammonium bicarbonate in 98 % methanol) mobile phase conditions, optimized for positive and negative electrospray ionization, respectively. After injection of the sample extracts the columns were developed in a gradient of 100 % A to 98 % B in 11 min runtime at 350 μl/min flow rate. The eluent flow was directly connected to the ESI source of the LTQ mass spectrometer. Full scan mass spectra (99–1000 m/z) and data dependent MS/MS scans with dynamic exclusion were recorded in turns.
GC/MS analysis was done on a Thermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer, equipped with a 20 m × 0.18 mm GC column with 0.18 μm film phase consisting of 5 % phenyldimethyl silicone. Electron impact ionization at 70 eV was used and the column temperature was ramped between 60 and 340 °C with helium as carrier gas. Mass spectra in a scan range from 50 to 750 m/z, were recorded.
Metabolites were identified from the LC/MS and GC/MS data by automated multiparametric comparison with a proprietary library, containing retention times, m/z ratios, and related adduct/fragment spectra for over 1,500 standard compounds measured by Metabolon. For each identified metabolite the raw area counts were normalized to the median value of the run day to correct for inter-day variation of the measurements.
The panel includes 517 untargeted metabolites, spanning several metabolic classes (amino acids, acylcarnitines, sphingomyelins, glycerophospholipids, carbohydrates, vitamins, lipids, nucleotides, peptides, xenobiotics and steroids). The quantified metabolites can be distinguished into chemically identified metabolites, and unidentified, here called “unknown” metabolites. Nine of those unknown metabolites have recently been identified by Krumsiek et al. (2012 (link)). Urate is one of the measured metabolites on the panel.
From the original data matrix containing 1,768 samples and 517 metabolites, we first excluded metabolites with more than 20 % missing values and then samples with more than 10 % missing values. The filtered data matrix contained n = 1,764 samples and 355 metabolites (241 known and 114 unknown). All normalized ion counts were transformed by natural logarithm and missing values were imputed using the ‘mice’ R package (van Buuren and Groothuis-Oudshoorn 2011 ). Detailed information about all analyzed metabolites is provided in Supplementary Table 1.
Publication 2013

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Publication 2017
acetonitrile ammonium acetate Biopharmaceuticals bropirimine Buffers Capillaries Cation Exchange Resins Cysteine formic acid High-Performance Liquid Chromatographies Ions Isotopes Light Methionine Mice, Laboratory Peptides polyetheretherketone Pressure Proteins Radionuclide Imaging Resins, Plant Silicon Dioxide Trypsin
Mass spectrometry was performed using a Thermo Orbitrap Velos mass spectrometer. Peptides were eluted using a 6-step MudPIT protocol (using 0%, 10%, 25%, 50%, 80%, and 100% salt bumps of 500 mM aqueous ammonium acetate, each step followed by an increasing gradient of aqueous acetonitrile/0.1% formic acid) and data were collected in data-dependent acquisition mode (2 MS1 microscans (400–1800 m/z) and 30 data-dependent MS2 scans) with dynamic exclusion enabled (repeat count of 1, exclusion duration of 20 s) with monoisotopic precursor selection enabled. All other parameters were left at default values. Unenriched samples were eluted in a 12-step MudPIT using (0%, 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100%) salt bumps of 500mM ammonium acetate. SEQUEST searches allowed for variable oxidation of methionine (+15.9949), static modification of cysteine residues (+57.0215 due to alkylation), and no enzyme specificity. Each data set was independently searched with light and heavy params files; for the light search, all other amino acids were left at default masses; for the heavy search, static modifications on lysine (8.0142) and arginine (10.0082) were specified. The precursor ion mass tolerance was set to 50 ppm and the fragment ion mass tolerance was left at the default assignment of 0. The data was searched using a mouse reverse-concatenated non-redundant (gene-centric) FASTA database that combines IPI and Ensembl identifiers, containing 23,420 unique entries. The resulting MS2 spectra matches were assembled into protein identifications and filtered using DTASelect (version 2.0.47) with the --trypstat option, which applies different statistical models for the analysis of tryptic, half-tryptic, non-tryptic peptides. Peptides were also restricted to fully tryptic using the -y 2 option with a defined peptide false positive rate of 2% (--fp 0.02) and single peptides per locus were allowed (-p 1). Redundant peptide identifications common between multiple proteins were allowed, but the database was restricted to a single consensus splice variant. SILAC ratios were quantified using in-house software as described12 (link). In short, extracted MS1 ion chromatograms (+/− 10 ppm) from both “light” and “heavy” target peptide masses (m/z) are generated using a retention time window (+/− 10 minutes) centered on the time when the peptide ion was selected for MS/MS fragmentation, and subsequently identified. Next, the ratio of the peak areas under the light and heavy signals (signal-to-noise ratio S/N >2.5) are calculated. Multiple computational filters are used to ensure that the correct peak-pair is used for quantification, including a co-elution correlation score filter (R2 ≥ 0.8) that removes target peptides with bad co-elution profile, and an “envelope correlation score” filter (R2 > 0.8) that eliminates target peptides whose predicted pattern of the isotopic envelope distribution does not match the experimentally observed high-resolution MS1 spectrum. Additionally, the software was updated to identify cases where complete inhibition could not be quantified based on light/heavy peak pairs due the absence of a MS1 signal from either the heavy or light sample. In order to identify these cases, all single MS1 chromatographic peaks (from either the light or the heavy sample) were identified within a retention time window. Next, these peaks were aligned with the corresponding sequence SEQUEST/DTASelect identification and the charge state and monoisotopic mass were validated using the “envelope correlation score” filter12 (link). Finally, the candidate peak was cross-checked to ensure there was no corresponding (heavy or light) peak co-eluting around the same retention time window. Only after all these conditions are met, the peptide was assigned as the case of complete inhibition with an artificial threshold ratio of 20. To identify 17-ODYA enriched proteins, two experiments were performed. Light and heavy cells were treated with 17-ODYA and palmitic acid respectively (N=5). The inverse experiment was next performed, where light cells were treated with palmitic acid and heavy cells with 17-ODYA (N=5). If the median peptide ratio pooled from both experiments was greater or equal to 1.5, and there were quantified peptides from both reciprocal experiments, the protein is considered to be palmitoylated. In subsequent experiments, singleton values were not included in mean calculations or in the number of quantitated peptides used for calculating standard errors.
Synthetic methods
Synthetic methods are described in the Supplementary Note.
Publication 2011

Most recents protocols related to «Formic acid, ammonium salt»

Ammonium acetate (≥98% purity, for
HPLC, acetic acid ammonium salt) and formic acid (≥98% purity)
were purchased from Acros Organics (Geel, Belgium). Methanol (for
LC) was purchased from Merck (Darmstadt, Germany). Ammonium hydroxide
solution (30–33% in water, w/w) was purchased from Sigma-Aldrich
(St. Louis, MO, USA). Ammonium acetate (10 mM) and formic acid (0.5%,
v/v) were prepared in 25% (v/v) methanol in deionized water. 225 μL
of ammonium hydroxide solution was mixed with 29.8 mL of 25% (v/v)
methanol in deionized water in order to achieve an approximate volumetric
concentration of 0.5% ammonium hydroxide.
Publication 2024
Not available on PMC !
Mobile phase A: Approximately 500 mL of Millipore water was taken in a 1000 mL measuring cylinder. After that 5 mL of formic acid and 630.559 mg ammonium formate salt were added to it. Finally, the volume was made up to 1000 mL with millipore water which produced 0.5% (v/v) formic acid containing 10 mM ammonium formate buffer solution. The mixture was filtered using a vacuum pressure pump fitted with 0.45 µm multiple N66 nylon 6,6 membrane. The solvent mixture was sonicated for 30 minutes to remove the air bubbles.
Mobile phase B: 100% methanol was taken in a 1000 mL measuring cylinder. Then the methanol was sonicated for 15 minutes to remove the air bubbles present in it.
Publication 2024
MS-grade solvents used for UPLC analysis acetonitrile (MeCN) water (H2O) and formic acid (HCOOH) were provided by Romil (Cambridge, UK); analytical-grade solvents methanol (MeOH) and ethanol (EtOH) were supplied by Sigma-Aldrich (Milan, Italy). H2O was purified by using a Milli-Q system (Millipore, Bedford, USA). Acetic acid (AA), ammonium hydroxide, naphthylethylene diamine dihydrochloride, phosphoric acid, ascorbic acid, fluorescein sodium salt, Trizma hydrocloride (Tris-HCl) monopotassium phosphate dipotassium phosphate, sodium nitroprusside dehydrate (SNP), sulphanilamide were provided by Sigma-Aldrich (Milan, Italy). 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH) were purchased from TCI Chemicals (Tokyo, Japan). Glucoarabinin potassium salt, glucocamelin potassium salt and homoglucocamelinin potassium salt were purchased from Extrasynthese (Lyion, France).
Publication 2024

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Publication 2024
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CML (≥ 95%) and CEL (≥ 95%) were purchased from Cayman Chemical (MI, USA). Other AGEs standards, namely pentosidine TFA salt (≥ 99%), methylglyoxal-hydroimidazolone isomers [MG-H1 acetate salt (≥ 96%), MG-H2 acetic acid salt (≥ 94%), MG-H3 TFA salt (≥ 99%)], argpyrimidine TFA salt (≥ 99%), glyoxal-hydroimidazolone isomers (G-H1, ≥ 98%), GOLD acetate salt (≥ 98%), MOLD acetate salt (≥ 95%), and the other isotopically labeled internal standards including CML-D 4 (≥ 99%), CEL-D 4 (≥ 99%), MG-H1-D 3 acetate salt (≥ 98%), G-H1-13 C2 (≥ 98%), and GOLD-15 N2 acetic acid salt (≥ 94%) were supplied by Iris Biotech GmnH (Marktredwitz, Germany). LC-MS grade ammonium formate (AF), formic acid (FA), and acetonitrile (ACN) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Ultrapure (UP) water purified from a Millipore Milli-Q Advantage A10 Water Purification System (Bedford, MA, USA) was used throughout. All other chemicals and reagents were of analytical grade and were purchased from Aladdin Industrial Co. (Shanghai, China) unless otherwise specified.
Publication 2024

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Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.
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Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.
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Formic acid is a clear, colorless liquid chemical compound used in various industrial and laboratory applications. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid has a pungent odor and is highly corrosive. It is commonly used as a preservative, pH adjuster, and analytical reagent in laboratory settings.
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Ammonium formate is a chemical compound that is commonly used in various laboratory applications. It is a crystalline solid that is soluble in water and other polar solvents. Ammonium formate serves as a buffer in analytical techniques and is also used as a mobile phase additive in liquid chromatography.
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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Ammonium acetate is a chemical compound with the formula CH3COONH4. It is a colorless, crystalline solid that is soluble in water and alcohol. Ammonium acetate is commonly used in various laboratory applications, such as pH adjustment, buffer preparation, and as a mobile phase component in chromatography.
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Methanol is a colorless, volatile, and flammable liquid chemical compound. It is commonly used as a solvent, fuel, and feedstock in various industrial processes.
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Acetonitrile is a highly polar, aprotic organic solvent commonly used in analytical and synthetic chemistry applications. It has a low boiling point and is miscible with water and many organic solvents. Acetonitrile is a versatile solvent that can be utilized in various laboratory procedures, such as HPLC, GC, and extraction processes.
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Ammonium bicarbonate is a chemical compound with the formula (NH4)HCO3. It is a white crystalline solid that is commonly used as a leavening agent in baking and as a source of carbon dioxide in certain industrial processes.
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Acetic acid is a colorless, vinegar-like liquid chemical compound. It is a commonly used laboratory reagent with the molecular formula CH3COOH. Acetic acid serves as a solvent, a pH adjuster, and a reactant in various chemical processes.

More about "Formic acid, ammonium salt"

Formic acid, ammonium salt, also known as ammonium formate, is a chemical compound with the molecular formula CH3NO2.
It is the ammonium salt of formic acid, a carboxylic acid found naturally in many plants and insects.
This versatile compound has a variety of industrial and research applications, including its use as a preservative, antimicrobial agent, and pH adjuster.
Researchers may find formic acid, ammonium salt useful in a wide range of experimental protocols, such as those involving organic synthesis, biochemical assays, and material science.
The compound can be employed as a buffer, neutralizing agent, or precursor in various chemical reactions and processes.
In addition to formic acid, ammonium salt, related compounds like acetonitrile, ammonium acetate, and ammonium bicarbonate may also be of interest in certain research contexts.
These substances can be utilized for tasks such as sample preparation, mobile phase adjustment, and enzyme inhibition, among others.
Leveraging the power of AI-driven platforms like PubCompare.ai can help researchers locate optimal protocols and procedures for working with formic acid, ammonium salt and related compounds.
By enhancing reproducibility and optimizing experiments, these tools can streamline the research process and lead to more reliable and impactful outcomes.