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Formic acid, sodium salt

Formic acid, sodium salt is a chemical compound with the formula HCOONa.
It is a white, crystalline solid that is widely used in various industrial and agricultural applications.
This salt is soluble in water and has a range of uses, including as a preservative, pH regulator, and in the production of other chemicals.
The PubCompare.ai platform can help researchers optimize their protocols for working with formic acid, sodium salt by providing access to relevant literature, preprints, and patent information, as well as intelligent comparisons to identify the best protocols and products.
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Most cited protocols related to «Formic acid, sodium salt»

The following commercial chemicals were used: ortho phosphoric acid (p.a., or TraceSELECT Ultra) from Fluka (Buchs, Switzerland); pyridine from Merck (Merck, Darmstadt, Germany); and hydrogen peroxide 30 % (p.a.), aqueous ammonia 25 % (suprapure), 65 % nitric acid (p.a.), and formic acid (p.a.) from Roth (Carl Roth, Karlsruhe, Germany). Chemicals were used without further purification except for the nitric acid which was distilled in a quartz sub-boiling distillation unit. Water used throughout was from a Milli-Q Academic water purification system (Millipore GmbH, Vienna, Austria) with a specific resistivity of 18.2 MΩ*cm.
Individual standard solutions (1000 ± 3 μg L−1 in 2 % nitric acid) for total element determinations of As, Cd, Mo, Pb, Sb, Se, U, W, and Zn (in the urine samples) and Ge, In, and Lu (internal standards) were obtained from CPI International (Santa Rosa, CA, US). For arsenic speciation, stock solutions containing 1000 mg As L−1 of each of the following species were prepared in water: arsenite (As(III) and arsenate (As(V)) prepared from NaAsO2 and Na2HAsO4.7 H2O, respectively, purchased from Merck (Darmstadt, Germany); dimethylarsinate (DMA) prepared from sodium dimethylarsinate purchased from Fluka (Buchs, Switzerland); methylarsonate (MA) prepared in-house from sodium arsenite and methyl iodide (Meyer reaction); and arsenobetaine (AB), as the bromide salt, prepared in-house following the method of Cannon et al.11 The purity of the synthesized standards (MA and AB) was established by NMR and HPLC/mass spectrometry. Other arsenic standards (trimethylarsine oxide, arsenocholine, tetramethylarsonium ion, oxo and thio-dimethylarsinylethanol and oxo- and thio-dimethylarsinylacetic acid) were prepared as previously reported;12 ,13 (link) these standards were used to check the identity of minor peaks which occasionally appeared in the chromatograms.
The certified reference materials for total element measurements were NIST 1643e, trace elements in water (National Institute of Standards & Technology, Gaithersburg, Maryland, US) certified for As, Cd, Mo, Pb, Sb, Se, & Zn; and NIES No. 18, human urine (National Institute for Environmental Studies, Tsukuba, Japan) certified for As, Se & Zn. In addition, Seronorm control urine (Sero AS, Billingstad, Norway) and an in-house urine sample served as non-certified reference materials. The certified reference material for determining arsenic species was NIES No 18, human urine, certified for AB and DMA. Our in-house reference urine was used as a control for iAs, MA, DMA, and AB.
Publication 2012
Acids Ammonia arsenate Arsenic arsenite arsenobetaine arsenocholine Bromides Cacodylate Distillation formic acid High-Performance Liquid Chromatographies Homo sapiens Mass Spectrometry methylarsonate methyl iodide Nitric acid Peroxide, Hydrogen Phosphoric Acids pyridine Quartz Rosa Sodium sodium arsenite Sodium Chloride tetramethylarsonium Trace Elements trimethylarsine oxide Urine
Strains were grown as described above. Culture samples (2 ml) were taken in mid-exponential and transition-to-stationary phases of growth, centrifuged (16,000×g, 2 min, 4°C), filtered (Millex-GN 0,22 µm filters) and the supernatant solutions were stored at −20°C until analysis by high performance liquid chromatography (HPLC). Prior to analysis, samples were allowed to thaw at room temperature. Glc or Gal and end-products were quantified in an HPLC apparatus equipped with a refractive index detector (Shodex RI-101, Showa Denko K. K.) using an HPX-87H anion exchange column (Bio-Rad Laboratories Inc.) at 60°C, with 5 mM H2SO4 as the elution fluid and a flow rate of 0.5 ml min−1. Alternatively, quantification of metabolites in the supernatant solutions was performed by 1H-NMR in a Bruker Avance II 500 MHz spectrometer (Bruker BioSpin GmbH). Formic acid (sodium salt) was added to the samples and used as an internal concentration standard. The ATP yield was calculated as the ratio of ATP produced to Glc or Gal consumed. The global yields of ATP were calculated from the fermentation products determined at the time-point of growth arrest assuming that all ATP was synthesized by substrate-level phosphorylation. A factor of 0.39, determined from a DW (mg ml−1) versus OD600 curve, was used to convert OD600 into dry weight (mg biomass ml−1).Hydrogen peroxide was quantified in fresh supernatant solutions using the Amplex® Red hydrogen Peroxide/Peroxidase assay kit from Invitrogen.
Publication 2011
1H NMR A-factor (Streptomyces) Anions Biological Assay Fermentation formic acid High-Performance Liquid Chromatographies Peroxidase Peroxide, Hydrogen Phase Transition Phosphorylation Sodium Sodium Chloride
The reversed phase chromatographic conditions were adapted from Hu et al. (2008 (link)). Methanol was preferred to acetonitrile since it has been observed that it decreased the carryover of most apolar lipid species (data not shown). CSF total lipid extracts were separated on an Dionex Ultimate 3000 UPLC system (Thermo Scientific, San Jose, CA) using a kinetex C8 150 × 2.1 mm, 2.6 μm column (Phenomenex, Sydney, NSW, Australia). Mobile phase A consisted of H2O/MeOH 60/40 (v/v) and 0.1 % formic acid and mobile phase B of IPA/MeOH 90/10 (v/v) and 0.1 % formic acid. Ammonium formate (10 mM) was added to both mobile phases in the positive ion mode. The presence of ammonium salt in mobile phases decrease drastically the detection of sodium adducts which now constitute minor lipid species in the positive ion mode (data not shown). The gradient program was as follows: solvent B was maintained for 2.5 min at 32 %, from 2.5 to 3.5 min it was increased to 45 % B, from 3.5 to 5 min to 52 % B, from 5 to 7 min to 58 % B, from 7 to 10 min to 66 % B, from 10 to 12 min to 70 % B, from 12 to 15 min to 75 % B, from 15 to 19 min to 80 % B, from 19 to 22 min to 85 % B, and from 22 to 23 min to 95 % B; from 23 to 25 min, 95 % B was maintained; from 25 to 26 min solvent B was decreased to 32 % and then maintained for 4 min for column re-equilibration. The flow rate was 400 μL/min and the column temperature was set to 60 °C.
Publication 2016
acetonitrile Chloride, Ammonium Chromatography, Reverse-Phase formic acid formic acid, ammonium salt Lipids Methanol Sodium Solvents
All α-thrombin proteins were prepared for HDXMS from frozen aliquots (~7 uM). After being passed through a 0.2 micron filter, a portion was diluted to 5 μM, and 130 μL was saved for the HDXMS experiment. The remaining sample was concentrated to 10 μM using pre-rinsed 6 mL 10K MWCO Amicon concentrators, spinning at 3000 rpm in 5 min intervals at 4°C to be used for peptide identification (50 μL). In the HDXMS experiment, the sample is diluted 12-fold (see below) resulting in a final α-thrombin concentration of 420 nM.
HDXMS was performed using a Waters Synapt G2Si system with HDX technology (Waters Corporation). Deuterium exchange reactions were prepared using a Leap HDX PAL autosampler (Leap Technologies, Carrboro, NC). D2O buffer was prepared by lyophilizing 1 mL of 250 mM phosphate pH 6.5 and either 1 M NaCl for low salt experiments of 3 M NaCl for high salt experiments, before being resuspended in 10 ml 99.96% D2O immediately before use. Each deuterium exchange time point (0 min, 30 sec, 1 min, 2 min, 5 min) was measured in triplicate. For each deuteration time point, 5 μL of protein was held at 25°C for 5 min before being mixed with 55 μL of D2O buffer. The deuterium exchange was quenched for 1 min at 1°C by combining 50 μL of the deuteration reaction with 50 μL of 250 mM TCEP pH 2.5. The quenched sample was then injected in a 50 μL sample loop, followed by digestion on an in-line pepsin column (immobilized pepsin, Pierce, Inc.) at 15°C. The resulting peptides were captured on a BEH C18 Vanguard pre-column, separated by analytical chromatography (Acquity UPLC BEH C18, 1.7 uM, 1.0 × 50 mm, Waters Corporation) using a 7-85% acetonitrile in 0.1% formic acid over 7.5 min, and electrosprayed into the Waters Synapt G2Si quadrupole time-of-flight mass spectrometer. The mass spectrometer was set the collect data in the Mobility, ESI+ mode; mass acquisition range of 200-2,000 (m/z); scan time 0.4 s. Continuous lock mass correction was accomplished with infusion of leu-enkephalin (m/z = 556.277) every 30 s (mass accuracy of 1 ppm for calibration standard).
For peptide identification, the mass spectrometer was set to collect data in MSE, mobility ESI+ mode instead. Peptides masses were identified from triplicated analyses of 10 μM α-thrombin, and data were analyzed using PLGS 2.5 (Waters Corporation). Peptides masses were identified using a minimum number of 250 ion counts for low energy peptides and 50 ion counts for their fragment ions; the peptides also had to be larger than 1500 Da. The following cutoffs were used to filter peptide sequence matches: minimum products per amino acid of 0.2, minimum score of 7, maximum MH+ error of 5 ppm, a retention time RSD of 5%, and the peptides had to be present in two of the three ID runs collected. The peptides identified in PLGS were then analyzed in DynamX 3.0 (Waters Corporation). The relative deuterium uptake for each peptide was calculated by comparing the centroids of the mass envelopes of the deuterated samples with the undeuterated controls following previously published methods (20 (link)). To account for back-exchange, and systematic autosampler sample handling differences between shorter and longer deuteration times, the uptake and standard deviation values for the 30 sec and 1 min, and the 2 min and 5 min timepoints were divided by 0.67 and 0.64 respectively for every HDXMS experiment run. Data were plotted as number of deuterons incorporated vs. time (min). The Y-axis limit for each plot reflects the total number of amides within the peptide that can possible exchange. Each plot includes the peptide MH+ value, sequence, and sequential residue numbering.
To monitor sodium binding, the HDXMS experiments were conducted at either 100 or 300 mM NaCl. Under these conditions of the HDXMS experiment (420 nM thrombin), the sodium binding site should have been less than 80% occupied at 100mM NaCl and over 90% occupied at 300 mM NaCl (21 (link)). Our previous studies have shown that for weak binding ligands, observation of “protection” of the surface of the protein requires the binding site to be over 90% occupied (22 (link)). Therefore, we expected to see the largest difference in surface “protection” by comparing 80% bound to >90% bound sodium.
Publication 2018
Neu5Ac, Neu5Gc and Neu5Ac9Az nonulosonates were purchased from commercial sources (Inalco Pharmaceuticals, Sigma, Sussex Research Laboratories Inc.). Neu5Ac9Ac and Neu5Gc8Me were synthesized using published methods [52 (link),53 (link)]. Leg5Ac7Ac and Pse5Ac7Ac were prepared enzymatically using methods in [38 (link)] and [37 (link)], respectively. CMP-activation of nonulosonate sugars was performed enzymatically using appropriate CMP-Sia, CMP-legionaminic acid and CMP-pseudaminic acid synthetases from either N. meningitidis, C. jejuni or Helicobacter pylori [37 (link),38 (link),54 (link),55 (link),56 (link)]. Reactions contained 50 mM Tris pH 8–9, 50 mM MgCl2, 15.7 mM CTP, 15 mM nonulosonate, 4 units pyrophosphatase per mM of CTP, and sufficient quantities of CMP-NulO synthetase enzyme to obtain optimal conversion at 5–6 hours. CMP-NulO enzymatic reaction mixtures were then passed through an Amicon Ultra-15 (10,000 molecular weight cut-off) or Ultra-4 (5,000 molecular weight cut-off) filter membrane before purification. Filtered CMP-NulO samples were then lyophilized and desalted/purified using a Superdex Peptide 10/300 GL (GE Healthcare) column in ammonium bicarbonate or NaCl solutions. To achieve additional purity, elution fractions containing individual CMP-NulOs were subjected to anion-exchange chromatography (Mono Q 4.6/100 PE, GE Healthcare) using either an ammonium bicarbonate or NaCl gradient. When NaCl gradients were used, CMP-NulOs were ‘desalted’ by gel filtration (Superdex Peptide 10/300 GL) using 1 mM NaCl. Quantification of CMP-NulO preparations was determined using the molar extinction coefficient of CMP (ε260 = 7,400). Prior to lyophilization, CMP-NuIOs were suspended in sodium hydroxide or NaCl solutions at molar ratios of 1:2 (NuIO: salt).
Purified CMP-nonulosonates were dissolved in >99% D2O and structural analysis performed by mass spectroscopy (MS) using either a Varian Inova 500 MHz (1H) spectrometer with a Varian Z-gradient 3 mm probe or a Varian 600 MHz (1H) spectrometer with a Varian 5 mm Z-gradient probe. All spectra were referenced to an internal acetone standard (δH 2.225 ppm and δC 31.07 ppm). Results that are shown in S1 Table and S1 Fig verify the production and purity of each CMP-nonulosonate compound made for this study; CMP-LegAc7Ac and CMP-Pse5Ac7Ac, which were confirmed based on NMR data presented in [38 (link)] and [37 (link)].
CMP-NulOs were also characterized using capillary electrophoresis (CE)-MS analysis. Separation of ions was achieved by CE (Prince CE system [Prince Technologies, Netherlands]) in a 90 cm long bare fused-silica capillary (365 μm OD x 50 μm ID). The 30 mM morpholine aqueous running CE buffer (adjusted to pH9 with formic acid) was coupled with the capillary sheath fluid (isopropanol: methanol [2:1]) at their interface prior to mass spectrometry (API3000 mass spectrometer [Applied Biosystems/Sciex, Concord, ON, Canada]). S2 Table indicates measured m/z ions of each CMP-nonulosonate compound using CE-MS. Measurements correspond to calculated masses.
Publication 2015
5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-non-2-ulosonic acid 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno nonulosonic acid Acetone ammonium bicarbonate Anions Buffers Capillaries Chromatography Electrophoresis, Capillary Enzymes Extinction, Psychological formic acid Freeze Drying Gel Chromatography Helicobacter pylori Ions Isopropyl Alcohol legionaminic acid Ligase Magnesium Chloride Mass Spectrometry Methanol Molar Mono Q Morpholines Neisseria meningitidis Peptides Pharmaceutical Preparations Pyrophosphatase Silicon Dioxide Sodium Chloride Sodium Hydroxide Sugars Tissue, Membrane Tromethamine

Most recents protocols related to «Formic acid, sodium salt»

MS-grade solvents used for UPLC analysis acetonitrile (MeCN) water (H2O) and formic acid (HCOOH) were provided by Romil (Cambridge, UK); analytical-grade solvents methanol (MeOH) and ethanol (EtOH) were supplied by Sigma-Aldrich (Milan, Italy). H2O was purified by using a Milli-Q system (Millipore, Bedford, USA). Acetic acid (AA), ammonium hydroxide, naphthylethylene diamine dihydrochloride, phosphoric acid, ascorbic acid, fluorescein sodium salt, Trizma hydrocloride (Tris-HCl) monopotassium phosphate dipotassium phosphate, sodium nitroprusside dehydrate (SNP), sulphanilamide were provided by Sigma-Aldrich (Milan, Italy). 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH) were purchased from TCI Chemicals (Tokyo, Japan). Glucoarabinin potassium salt, glucocamelin potassium salt and homoglucocamelinin potassium salt were purchased from Extrasynthese (Lyion, France).
Publication 2024
Phenoxymethylpenicillin potassium salt (penicillin-V), benzylpenicillin sodium salt (penicillin-G), probenecid and benzylpenicillin-d7 N-ethylpiperidinium salt (IS) were purchased from Sigma (St. Louis, MO, USA). Probenecid-d14 was purchased from Cambridge Bioscience (Cambridge, UK) (Fig. 1). Methanol, formic acid, water and acetonitrile in LC/MS grade were purchased from Sigma (St. Louis, MO, USA).
Publication 2024
Acetonitrile, ammonium acetate, isopropanol, and methanol (> 99.9%) were all purchased from VWR Chemicals (Leuven, Belgium). Formic acid was obtained from Fischer Scientific (Waltham, MA, USA) and sodium taurocholate hydrate (96%) was purchased from Alfa Aesar (Kandel, Germany). Sodium glycocholate hydrate (> 98%) was bought from abcr (Karlsruhe, Germany) and lysophosphatidylcholine from soybean was generously donated from Lipoid (Ludwigshafen, Germany). Chenodeoxycholic acid (≥ 96%), cholesterol (≥ 99%), cholic acid (≥ 98%), hyodeoxycholic acid (≥ 98%), linoleic acid (≥ 99%), linolenic acid (≥ 99%), lysophosphatidylcholine from egg yolk (European Pharmacopeia reference standard), oleic acid (≥ 99%), palmitic acid (≥ 99%), phosphatidylcholine from egg yolk (European Pharmacopeia reference standard), phosphatidylcholine from soya bean (European Pharmacopeia reference standard), phosphatidylethanolamine from soya bean (European Pharmacopeia reference standard), sodium deoxycholate (≥ 98%), sodium glycochenodeoxycholate (≥ 97%), sodium glycodeoxycholate (≥ 97%), sodium taurochenodeoxycholate (≥ 95%), sodium taurodeoxycholate hydrate (≥ 95%), sphingomyelin from chicken egg yolk (≥ 98.0%), stearic acid (approx. 99%), tauro-α-muricholic acid sodium salt (> 99%), tauro-β-muricholic acid sodium salt (> 99%), ursodeoxycholic acid (≥ 99%), α-muricholic acid (> 99%), β-muricholic acid (≥ 98%), and ω-muricholic acid (> 99%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Purified water was prepared from a SG ultra-clear UV apparatus from Holm & Halby Service (Brøndby, Denmark).
Publication 2024

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Publication 2024
Merck (Darmstadt, Germany), Sigma Aldrich (Steinheim, Germany), and Fluka (Buchs, Switzerland) provided the LC-MS-grade chemicals required to prepare the electrolyte solutions (i.e., ammonium carbonate—(NH4)2CO3, ammonium hydrogen carbonate—NH4HCO3), as well as sheath liquid solutions (methanol—MeOH, isopropyl alcohol—IP, formic acid, and ammonium formate). LC-MS-grade acetonitrile (ACN) was obtained from VWR International (Vienna, Austria). Sodium hydroxide (NaOH), p.a. quality, was obtained from Agilent Technologies (Santa Clara, CA, USA). Dimethyl sulfoxide (DMSO), p.a. quality, was obtained from Sigma Aldrich. The electrolytes, sheath liquid, and samples were prepared using demineralized water, which was produced using a Direct-Q® 3 UV water purification system (Millipore, Molsheim, France). Electrolytes were kept in the refrigerator before analysis and filtered using disposable membrane filters with a 0.22 μm pore size from Millipore.
Analytical-grade standards of investigated ATBs (amoxicillin, ampicillin, cefotaxime sodium salt, ceftazidime, flucloxacillin sodium salt, meropenem trihydrate, oxacillin sodium monohydrate, piperacillin sodium salt, sulbactam sodium salt, and tazobactam) were purchased from Sigma Aldrich. The deuterated internal standards ([13C, 2H3]-cefotaxime, [2H6]-meropenem, [2H5]-piperacillin sodium salt, and [13C2, 15N3]-tazobactam sodium salt) were purchased from Alsachim (Strasbourg, France).
Publication 2024

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Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.
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Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.
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Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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Acetonitrile is a highly polar, aprotic organic solvent commonly used in analytical and synthetic chemistry applications. It has a low boiling point and is miscible with water and many organic solvents. Acetonitrile is a versatile solvent that can be utilized in various laboratory procedures, such as HPLC, GC, and extraction processes.
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Methanol is a colorless, volatile, and flammable liquid chemical compound. It is commonly used as a solvent, fuel, and feedstock in various industrial processes.
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Formic acid is a clear, colorless liquid chemical compound used in various industrial and laboratory applications. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid has a pungent odor and is highly corrosive. It is commonly used as a preservative, pH adjuster, and analytical reagent in laboratory settings.
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Sodium carbonate is a water-soluble inorganic compound with the chemical formula Na2CO3. It is a white, crystalline solid that is commonly used as a pH regulator, water softener, and cleaning agent in various industrial and laboratory applications.
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Acetic acid is a colorless, vinegar-like liquid chemical compound. It is a commonly used laboratory reagent with the molecular formula CH3COOH. Acetic acid serves as a solvent, a pH adjuster, and a reactant in various chemical processes.
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Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.

More about "Formic acid, sodium salt"

Formic acid, sodium salt, also known as sodium formate, is a versatile chemical compound with the molecular formula HCOONa.
It is a white, crystalline solid that is widely used in various industrial and agricultural applications.
This salt is soluble in water and has a range of applications, including as a preservative, pH regulator, and in the production of other chemicals like acetic acid, methanol, and sodium carbonate.
Researchers working with formic acid, sodium salt can optimize their protocols by utilizing the PubCompare.ai platform.
This AI-driven platform provides access to relevant literature, preprints, and patent information, as well as intelligent comparisons to identify the best protocols and products.
This streamlines the research process and allows for more efficient and effective experimentation.
In addition to formic acid, sodium salt, researchers may also be interested in exploring related compounds like formic acid, acetonitrile, gallic acid, and sodium hydroxide.
These substances can have complementary or overlapping uses, and the PubCompare.ai platform can help researchers navigate the complex landscape of chemical research and development.
By leveraging the power of PubCompare.ai's tools and resources, researchers can optimize their workflows, save time, and ultimately drive more impactful discoveries in the field of chemistry and beyond.