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Fucoxanthin

Fucoxanthin is a carotenoid pigment found in various types of brown algae and diatoms.
It has demonstrated potential health benefits, including antioxidant, anti-inflammatory, and anti-obesity properties.
Researchers can leverage the PubCompare.ai platform to enhance their Fucoxanthin studies by identifying the most accurate and reproducibile protocols from the literature, pre-prints, and patents.
This AI-driven tool leverages advanced technology to empower researchers, enabling them to make informed decisions and drive their Fucoxanthin research forward more effectively.

Most cited protocols related to «Fucoxanthin»

1
Extraction and Purification of Fucoxanthin from Diatom
Cited 5 times
The P. tricornutum cells in different culture stages were centrifuged at 4000× g, then rinsed with ddH2O, and recollected by centrifugation. Pellets were suspended in ethanol for pigment extraction (ethanol:algae culture volume = 1:1; v/v). In agreement with others, we found ethanol to be the most effective solvent in the extraction of fucoxanthin, with the extraction yield being ethanol > acetone > ethyl acetate [20 (link)]. The extraction system was incubated at 45 °C for 2 h, and mixed by vortex mixer every half an hour. Finally, the pigment solution was separated by centrifugation at 4000× g. The visible spectrum of the pigment solution was obtained by scanning from 400 to 800 nm with a spectrometer (Perkin Elmer UV-VIS Spectrometer Lambda 25, Waltham, MA, USA).
Purification of the fucoxanthin was carried out by using solid phase extraction (SPE) columns (Agilent Bond Elut HF Mega BE-SI, 5 mg 20 mL, Santa Clara, CA, USA). The pigment extracts were dried under nitrogen and resuspended in the mobile phase (n-hexane:acetone = 6:4) [6 (link)]. The total pigments were loaded on the SPE columns, then eluted by the mobile phase. Chl a eluted first, followed by fucoxanthin.
Pigments purity was checked using silica plates (Merck TLC Silica gel 60, Darmstadt, Germany) using hexane:acetone = 6:4 as the mobile phase. The pigment spots were detected visually, scraped from the plate, and then resuspended in ethanol for spectrophotometric analysis.
Quantification of fucoxanthin by HPLC was accomplished using a HITACHI Primaide HPLC system (HITACHI, Tokyo, Japan) with a C18 reverse phase column (2.7 μm particle size, 100 × 4.6 mm). The mobile phase consisted of acetonitrile and water with a flow rate of 1 mL·min−1. After loading the column with the fucoxanthin extract in ethanol, the mobile phase was an acetonitrile:water solution with the ratio increasing from 80:20 to 100:0 over 8 min, maintained at 100:0 for 3 min, and then decreased back to 80:20 over 5 min. The chromatogram was recorded at 445 nm. Fucoxanthin standards (ChromaDex, fucoxanthin (P), ASB-00006296-010, Irvine, CA, USA) were used for the construction of standard curve in the concentration range of 0.01–1 mg∙mL−1.
Wang L.J., Fan Y., Parsons R.L., Hu G.R., Zhang P.Y, & Li F.L. (2018). A Rapid Method for the Determination of Fucoxanthin in Diatom. Marine Drugs, 16(1), 33.
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Publication 2018
Acetone acetonitrile Cell Culture Techniques Centrifugation Ethanol ethyl acetate Exanthema fucoxanthin High-Performance Liquid Chromatographies n-hexane Nitrogen Pellets, Drug Pigmentation Silica Gel Silicon Dioxide Solid Phase Extraction Solvents Spectrophotometry
2
Fucoidan and Fucoxanthin Extraction from Sargassum
Cited 4 times
LMF (Hi-Q Oligo-Fucoidans®) and HS-Fucox were derived from Sargassum hemiphyllum and prepared by Hi-Q Marine Biotech International Ltd. (New Taipei City, Taiwan). LMF was obtained by enzyme hydrolysis of original fucoidan. The characteristics of LMF-LJ were average molecular weight of 0.8 KDa (92.1%), fucose content 210.9±3.3 µmol/g, and sulfate content 38.9±0.4% (w/w). The extraction method followed the method mentioned before with technological modifications (24 (link)). HS-Fucox is a mixture of brown seaweed extract containing about 10% of fucoxanthin that is coated directly with polysaccharides of its own. It was dissolved in double-distilled H2O (ddH2O) and completely dissolved by stirring at room temperature for 30 min.
Hwang P.A., Phan N.N., Lu W.J., Ngoc Hieu B.T, & Lin Y.C. (2016). Low-molecular-weight fucoidan and high-stability fucoxanthin from brown seaweed exert prebiotics and anti-inflammatory activities in Caco-2 cells. Food & Nutrition Research, 60, 10.3402/fnr.v60.32033.
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Publication 2016
Enzymes fucoidan Fucose fucoxanthin Hydrolysis Marines Oligonucleotides Polysaccharides Sargassum Sulfates, Inorganic
3
Computational Study of Fucoxanthin Chromophore
Cited 4 times
The electronic properties of the fucoxanthin chromophore were studied by using MNDO-PSDCI,36 –40 (link) SAC-CI41 (link),42 and TD-DFT43 (link)–45 (link) molecular orbital theory. All calculations were based on a ground-state B3LYP/6-31G(d) minimized geometry.46 The MNDO-PSDCI calculations included the eight highest energy occupied π orbitals, the three highest energy occupied σ orbitals, the eight lowest energy unoccupied π orbitals and the lowest energy unoccupied σ orbital. Full single and double CI was carried out within the π system and full single CI within the σ system. In some calculations, quadruple configurations involving the π orbitals were introduced by using a coupled cluster perturbative approximation. The one photon spectra and the output from the molecular orbital calculations were analyzed by using MathScriptor, which also generated Figure 5 and Figure 6. The MNDO-PSDCI and MathScriptor programs are available by contacting RR Birge (rbirge@uconn.edu). The TD-DFT calculations used various functionals ranging from low-correlation PBE1PBE to the high correlation SVWN (or LSDA) functionals.44 ,45 (link) The SAC-CI calculations were carried out using the D95 basis set and included energy-selected single and double CI with the highest energy unoccupied and the lowest energy unoccupied orbitals.41 (link),42 The ground-state minimizations, SAC-CI and TD-DFT calculations were carried out within the Gaussian 03 program.46
Premvardhan L., Sandberg D.J., Fey H., Birge R., Büchel C, & van Grondelle R. (2008). The Charge-Transfer Properties of the S2 State of Fucoxanthin in Solution and in Fucoxanthin Chlorophyll-a/c2 Protein (FCP) Based on Stark Spectroscopy and Molecular-Orbital Theory. The journal of physical chemistry. B, 112(37), 11838-11853.
Publication 2008
fucoxanthin
4
Antimicrobial Activity of Fucoxanthin
Cited 3 times
The microbial growth inhibitory potential of the tested xanthophyll was determined by using the agar disc-diffusion method according to recommendations of the Clinical and Laboratory Standards Institute (CLSI) [39 ], and as described in our previous publication [40 (link)]. In brief, bacterial inocula of 0.5 McFarland were prepared. Next, 100 µL of all bacterial suspensions were inoculated on Mueller–Hinton agar with 5% sheep blood or Mueller–Hinton agar (Oxoid, Poland; Graso, Poland). Fucoxanthin was dissolved in 20% water solution of DMSO (Sigma-Aldrich, Poznań, Poland) in a final concentration of 1 mg/mL. A total of 25 µL of 1 mg/mL fucoxanthin (25 µg/disc) were transferred onto sterile filter papers (6 mm diameter). Additionally, sterile filter papers soaked 25 µL of 20% DMSO (negative control) were used. The plates were incubated at 35 °C for 18 h and anaerobes for two days. Results were shown as zones of growth inhibition (ZOIs). The experiments were repeated three times.
Minimal inhibitory concentration (MIC) was determined by the micro-dilution method using a 96-well plate (Nunc) according to CLSI [39 ]. Primarily, 100 µL of Mueller–Hinton broth, or Thioglicolate broth (Oxoid, Poland; Graso, Poland) for anaerobes, was placed in each well. The stock solution of fucoxanthin was transferred into the first well, and serial dilutions were performed so that concentrations in the range of 15.6 to 1000 µg/mL were obtained. The inoculums were adjusted to contain approximately 107 CFU/mL bacteria. 10 µL of the proper inoculums were added to the wells. Additionally, 10 μL of 0.2% aqueous solution of 2,3,5-triphenyltetrazolium chloride (TTC) was added to each well. TTC is converted in bacterial cells into red, insoluble formazan crystals [41 ]. Next, the plates were incubated at 35 °C for 24 h. The MIC value was taken as the lowest concentration of the extract that inhibited any visible bacterial growth. The experiments were repeated three times.
Karpiński T.M, & Adamczak A. (2019). Fucoxanthin—An Antibacterial Carotenoid. Antioxidants, 8(8), 239.
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Publication 2019
Agar Bacteria Bacteria, Anaerobic BLOOD Cells Clinical Laboratory Services Clinical Laboratory Techniques Diffusion Formazans fucoxanthin Lutein Minimum Inhibitory Concentration Psychological Inhibition Sheep Sterility, Reproductive Sulfoxide, Dimethyl Technique, Dilution triphenyltetrazolium chloride
5
Quantifying Photosynthetic Pigments in Microalgae
Cited 3 times
The extinction coefficient of the Chl a at 445 nm was calculated using a published extinction coefficient at 663 nm (ε663 nm = 82.04) [30 (link)] using Chl a prepared from various sources, including tobacco leaf, N. oceanica, and P. tricornutum. The absorbance values at 445 nm and 663 nm were measured and a calibration curve was established. This allowed for us to estimate the extinction coefficient of Chl a at 445 nm.
For detecting the concentration of fucoxanthin in P. tricornutum using a spectrophotometry, the cultures were diluted with culture medium, and the absorbance measured at 750 nm (A750 ranges from 0.1 to 0.8). In parallel, a volume of culture was centrifuged and the cells resuspended in an equal volume of ethanol, then the A445 and A663-values were detected after dilution with ethanol (A445 & A663 range from 0.2 to 1). Samples were protected from light exposure as much as possible using foil. The cells were suspended in ethanol and analyzed at A445 and A663 within 5 min. With this data, the concentration of fucoxanthin could be calculated using our formula.
A multi-mode microplate reader (SynergyTM HT, BioTek, Winooski, VT, USA) with 96-well plates was used to study the feasibility of high-throughput analysis following the method described above with the following modifications. The volume of samples in each well was 200 μL. We corrected to a 1 cm path-length using the software provided with the plate reader.
Wang L.J., Fan Y., Parsons R.L., Hu G.R., Zhang P.Y, & Li F.L. (2018). A Rapid Method for the Determination of Fucoxanthin in Diatom. Marine Drugs, 16(1), 33.
Full text: Click here
Publication 2018
Cells Culture Media Ethanol Extinction, Psychological fucoxanthin Light Nicotiana tabacum Plant Leaves Spectrophotometry Technique, Dilution

Most recents protocols related to «Fucoxanthin»

1
Fucoxanthin Stability Evaluation
Not available on PMC !
The fucoxanthin stability test was carried out using two samples, which were fucoxanthin and nano chitosan-pectin-fucoxanthin. A total of 10 mg fucoxanthin was dissolved in 50 mL of ethanol to obtain a concentration of 200 ppm. The fucoxanthin solution was left at 25°C for 5 days. At certain time intervals, a 3 mL sample was taken, and the absorbance was measured at a wavelength of 350-800 mm. The stability test of nano chitosan-pectin-fucoxanthin was conducted by modifying the Oliyaei et al. (2020) method at room temperature. A total of 50 mg of sample was put into a vial, covered by aluminum foil, and stored for 2 weeks. At certain intervals, a 3 mg sample was taken, and the fucoxanthin concentration was determined as explained in Subsection 2.7. Fucoxanthin degradation was calculated using Equation ( 2). The oxidation half-life of fucoxanthin was calculated via Equation (3).
(2)
where n T is the concentration of fucoxanthin at a certain time, W T is the weight of fucoxanthin at a certain time, W 0 is the initial weight of fucoxanthin, Nt is the amount of final fucoxanthin, N 0 is the amount of initial fucoxanthin, t is time of degradation, and t ½ is half-life of fucoxanthin oxidation.
Nahrowi R., Solehati S., Widyastuti W., Juliasih N.L.G.R., Pandiangan K.D., Setiawan A., & Hendri J. (2024). New Encapsulation of Fucoxanthin Isolated from Cyclotella striata by Nano Chitosan–Pectin using Ionic Gelation Method. Science & Technology Indonesia, 9(3), 517-528.
Publication 2024
2
Fucoxanthin Encapsulation in Nano Chitosan-Pectin
Not available on PMC !
Fucoxanthin encapsulation was carried out based on the method described by Koo et al. (2023) but with modification. The fucoxanthin powder was dissolved in ethanol so the concentration was 200 ppm. A total of 200 mL of nano chitosan-pectin solution was added to 15 mL of 200 ppm fucoxanthin solution dropwise. The process of adding the nano chitosan-pectin solution to the fucoxanthin solution was carried out for 1 hour with constant stirring. The nano chitosan-pectin-fucoxanthin solution was then frozen using a freezer for 40 hours and dried using a freeze dryer. The nano chitosan-pectin-fucoxanthin powder was then analyzed via encapsulation efficiency and stability test.
Nahrowi R., Solehati S., Widyastuti W., Juliasih N.L.G.R., Pandiangan K.D., Setiawan A., & Hendri J. (2024). New Encapsulation of Fucoxanthin Isolated from Cyclotella striata by Nano Chitosan–Pectin using Ionic Gelation Method. Science & Technology Indonesia, 9(3), 517-528.
Publication 2024
3
Purification of Fucoxanthin from Marine Algae
Not available on PMC !
The concentration of fucoxanthin in marine algae is relatively low, necessitating purification processes to enhance its purity and yield. Currently, common purification techniques include organic solvent extraction, chromatography, and crystallization [13, 14] .
Organic solvent extraction is a frequently employed method, utilizing solvents such as acetone, ethanol, n-butanol, and chloroform. By mixing the algal powder with the organic solvent, followed by stirring and oscillation, an extract containing fucoxanthin is obtained. Subsequently, the extract is centrifuged, and the supernatant is collected and concentrated using a rotary evaporator, resulting in a concentrated fucoxanthin solution.
Chromatography, another widely used purification technique, encompasses gel chromatography, reverse-phase high-performance liquid chromatography (RP-HPLC), and ion-exchange chromatography. Among these, RP-HPLC stands out as an effective method for achieving high-purity fucoxanthin. In RP-HPLC, a C18 column with hydrophobic groups is employed. Through interactions between fucoxanthin and the column, and by adjusting the polarity and flow rate of the elution solvent, fucoxanthin can be effectively separated.
Apart from these two primary purification methods, crystallization is also commonly utilized, capable of purifying fucoxanthin to over 99% purity. Solvents commonly used in the crystallization process include acetone, ethanol, n-butanol, and water. By carefully controlling the crystallization temperature and rate, fucoxanthin crystals with various purities and morphologies can be obtained.
Pang Y., Song W., & Lin F. (2024). Progress in the Preparation and Application of Fucoxanthin from Algae. International Journal of Biology and Life Sciences, 5(3), 46-51.
Publication 2024
4
Encapsulation Efficiency of Fucoxanthin
Not available on PMC !
Analysis of the encapsulation efficiency of fucoxanthin was obtained as a comparison of the mass of encapsulated fucoxanthin with the total mass of fucoxanthin used (Oliyaei et al., 2020) . A total of 15 mg of sample was added to 5 mL of ethanol and sonicated for 30 minutes. Approximately 3 mL of sample was taken, and the absorbance was measured using a UV-Vis spectrophotometer at 448 nm. Fucoxanthin concentration was determined based on a standard curve obtained from a standard solution with different concentrations. The encapsulation efficiency was determined based on the Equation (1).
Where EE is the encapsulation efficiency, W R is the actual weight of fucoxanthin, and W T is the theoretical weight of fucoxanthin.
Nahrowi R., Solehati S., Widyastuti W., Juliasih N.L.G.R., Pandiangan K.D., Setiawan A., & Hendri J. (2024). New Encapsulation of Fucoxanthin Isolated from Cyclotella striata by Nano Chitosan–Pectin using Ionic Gelation Method. Science & Technology Indonesia, 9(3), 517-528.
Publication 2024
5
Isolation and Purification of Fucoxanthin from C. striata Microalgae
Not available on PMC !
Isolation of fucoxanthin from C. striata microalgae was carried out through several steps, including cultivation, extraction, and purification. Initially, the stock solution of C. striata was cultivated in seawater media for 14 days referring to the modified method of Kusumaningtyas et al. (2017) . On the 14 th day after cultivation, the seawater culture was centrifuged to obtain C. striata microalgae biomass. A total of 6.1 g of biomass was extracted by 18 mL of ethanol and then centrifuged to separate extract and waste (Perez et al., 2019) . The fucoxanthin extract was purified using medium-pressure liquid chromatography (MPLC). Approximately 1 mL of ethanol extract was injected into the MPLC at a flow rate of 25 mL/min. The sample was then fractionated using SiO 2 as the stationary phase and ethanol as the mobile phase. The detector used was a photodiode array at wavelengths 220, 254, and 364 nm. The MPLC fractions of fucoxanthin were then characterized using a UV-Vis spectrophotometer and stored in a dark bottle.
Nahrowi R., Solehati S., Widyastuti W., Juliasih N.L.G.R., Pandiangan K.D., Setiawan A., & Hendri J. (2024). New Encapsulation of Fucoxanthin Isolated from Cyclotella striata by Nano Chitosan–Pectin using Ionic Gelation Method. Science & Technology Indonesia, 9(3), 517-528.
Publication 2024

Top products related to «Fucoxanthin»

1
Fucoxanthin by Merck Group
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Fucoxanthin is a carotenoid pigment found in brown algae and diatoms. It is a natural compound with antioxidant properties. Fucoxanthin is used as a research tool in biochemical and cell biology applications.
2
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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Dimethyl sulfoxide (dmso) by Merck Group
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.
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β carotene by Merck Group
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β-carotene is a carotenoid compound commonly used in laboratory research and product development. It functions as a provitamin, which means it can be converted into vitamin A in the body. β-carotene is a natural colorant and antioxidant with potential applications in various industries.
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Fucoxanthin standard by Merck Group
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Fucoxanthin standard is a reference material used for the identification and quantification of fucoxanthin, a naturally occurring carotenoid compound. It is typically used in analytical procedures, such as high-performance liquid chromatography (HPLC) or spectrophotometric analysis, to establish the presence and concentration of fucoxanthin in various samples.
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Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
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Trizol reagent by Thermo Fisher Scientific
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
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DPPH is a chemical compound used as a free radical scavenger in various analytical techniques. It is commonly used to assess the antioxidant activity of substances. The core function of DPPH is to serve as a stable free radical that can be reduced, resulting in a color change that can be measured spectrophotometrically.

More about "Fucoxanthin"

Fucoxanthin is a carotenoid pigment found in various types of brown algae and diatoms, such as Undaria pinnatifida, Sargassum fusiforme, and Phaeodactylum tricornutum.
This natural compound has demonstrated a range of potential health benefits, including antioxidant, anti-inflammatory, and anti-obesity properties.
Researchers can leverage the PubCompare.ai platform to enhance their Fucoxanthin studies by identifying the most accurate and reproducible protocols from the literature, pre-prints, and patents.
PubCompare.ai is an AI-driven tool that empowers researchers to make informed decisions and drive their Fucoxanthin research forward more effectively.
The platform utilizes advanced technology to locate and compare Fucoxanthin-related protocols from a variety of sources, including scientific journals, preprint servers, and patent databases.
This allows researchers to identify the most reliable and reproducible methodologies, saving time and resources.
When studying Fucoxanthin, researchers may also consider related compounds and materials, such as fetal bovine serum (FBS), dimethyl sulfoxide (DMSO), gallic acid, and β-carotene.
These substances can be used in conjunction with Fucoxanthin in cell culture experiments or as reference standards.
Additionally, the use of Fucoxanthin standard, penicillin/streptomycin, and TRIzol reagent may be relevant to Fucoxanthin research protocols.
By leveraging the insights and capabilities of PubCompare.ai, researchers can enhance their Fucoxanthin studies and make more informed decisions, ultimately driving their research forward and contributing to the scientific understanding of this promising natural compound.