Log In Sign up
The largest database of trusted experimental protocols
> Chemicals & Drugs > Organic Chemical > G 130

G 130

G 130 is a biomolecular compound with potential applications in various research and therapeutic fields.
This compound is known to have specific biological activities and interactions that may be of interest to researchers.
PubCompare.ai, an AI-driven analysis tool, can help locate the most effective G 130 protocols from literature, preprints, and patents, enhancing research reproducibility.
By leveraging powerful AI comparisons, scientists can identify the most effective protocols and products, streamlining their research process and improving overall reproducibility.
Discover the power of PubCompare.ai today and unlock the potential of G 130 in your research endeavors.

Most cited protocols related to «G 130»

1
Standardized Proteomic Sample Preparation Protocol
Cited 9 times
HEK293 cells (ATCC—low passage cells—not verified or mycoplasma tested) were cultured in DMEM (10% FCS, 50 μg ml−1 penicillin, 50 μg ml−1 streptomycin). HEK293 cells were selected as they are a common cell line used in molecular biology research with many published orthogonal data sets. Cell pellets were lysed on ice by using a lysis buffer containing 8 M urea (EuroBio), 40 mM Tris-base (Sigma-Aldrich), 10 mM DTT (AppliChem), and complete protease inhibitor cocktail (Roche). The mixture was sonicated at 4 °C for 5 min using a VialTweeter device (Hielscher-Ultrasound Technology) at the highest setting and centrifuged at 21,130×g, 4 °C for 1 h to remove the insoluble material. The supernatant protein mixtures were transferred and the protein amount was determined with a Bradford assay (Bio-Rad). Then five volumes of precooled precipitation solution containing 50% acetone, 50% ethanol, and 0.1% acetic acid were added to the protein mixture and kept at −20 °C overnight. The mixture was centrifuged at 20,400×g for 40 min. The pellets were further washed with 100% acetone and 70% ethanol with centrifugation at 20,400×g for 40 min. Aliquots of 2 mg protein mixtures were reduced by 5 mM tris(carboxyethyl)phosphine (Sigma-Aldrich) and alkylated by 30 mM iodoacetamide (Sigma-Aldrich). The samples were then digested with sequencing-grade porcine trypsin (Promega) at a protease/protein ratio of 1:50 overnight at 37 °C in 100 mM NH4HCO3 (ref. 70 (link)). Digests were combined together and purified with Sep-Pak C18 Vac Cartridge (Waters). The peptide amount was determined by using Nanodrop ND-1000 (Thermo Scientific). An aliquot of retention time calibration peptides from an iRT-Kit (Biognosys) was spiked into the sample at a ratio of 1:20 or 1:25 (v/v) to correct relative retention times between acquisitions71 (link).
Thirty heavy labeled synthetic peptides that were previously used in an SRM study focused on limits of detection in mammalian cells48 (link) were selected. As such, these peptides are expected to perform well in LC–MS analysis. The MS response for each peptide was measured. The peptides were ranked by MS response and assigned to five groups (A–E) to ensure there was a range of responses across in each group. These peptides groups were diluted into the matrix described above across a concentration range to create the five different samples to be analyzed (Fig 1a, Supplementary Tables 1 and 2). Finally, samples were shipped on dry ice to the 11 sites.
Collins B.C., Hunter C.L., Liu Y., Schilling B., Rosenberger G., Bader S.L., Chan D.W., Gibson B.W., Gingras A.C., Held J.M., Hirayama-Kurogi M., Hou G., Krisp C., Larsen B., Lin L., Liu S., Molloy M.P., Moritz R.L., Ohtsuki S., Schlapbach R., Selevsek N., Thomas S.N., Tzeng S.C., Zhang H, & Aebersold R. (2017). Multi-laboratory assessment of reproducibility, qualitative and quantitative performance of SWATH-mass spectrometry. Nature Communications, 8, 291.
Full text: Click here
Publication 2017
Acetic Acid Acetone Biological Assay Buffers Cell Lines Cells Centrifugation Dry Ice Ethanol G 130 HEK293 Cells Iodoacetamide Mammals Medical Devices Mycoplasma Pellets, Drug Penicillins Peptide Hydrolases Peptides phosphine Pigs Promega Protease Inhibitors Proteins Retention (Psychology) Sep-Pak C18 Staphylococcal Protein A Streptomycin Tromethamine Trypsin Ultrasonography Urea
2
Dissociation of human cortical tissue
Cited 9 times
hCSs were enzymatically dissociated to make a single cell suspension using a method used previously for dissociating brain tissue42 (link),43 (link). Briefly, the tissue was incubated at 33 °C for 45 min in 20 ml of a papain solution containing Earle’s balanced salts (EBSS, Sigma, E7510), D-(+)-glucose (22.5 mM), NaHCO3 (26 mM), DNase (125 U/ml, Worthington, LS002007), papain (30 U/ml, Worthington LS03126), and L-cysteine (1 mM, Sigma, C7880). The papain solution was equilibrated with 5% CO2 and 95% O2 gas before and during treatment. The tissue was subsequently washed three times with an inhibitor buffer containing BSA (1.0 mg/ml, Sigma A-8806) and ovomucoid (also known as trypsin inhibitor, 1.0 mg/ml, Roche Diagnostics Corporation 109878) and then mechanically dissociated by gentle sequential trituration. Dissociated cells were layered on top of high concentration inhibitor solution with 5 mg/ml BSA and 5 mg/ml ovomucoid and centrifuged at 130 g for five minutes. The cell pellet was then resuspended in Dulbecco’s phosphate-buffered saline (DPBS, Invitrogen, 14287) containing 0.02% BSA and 12.5U/ml DNase and filtered through a 20 μm Nitex mesh (Sefar America Inc., Lab Pak 03-20/14) to remove undissociated cell clumps. Cell health was assessed by trypan blue exclusion. Only single cell suspensions with > 85% viability were used for experiments.
Paşca A.M., Sloan S.A., Clarke L.E., Tian Y., Makinson C.D., Huber N., Kim C.H., Park J.Y., O’Rourke N.A., Nguyen K.D., Smith S.J., Huguenard J.R., Geschwind D.H., Barres B.A, & Paşca S.P. (2015). Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture. Nature methods, 12(7), 671-678.
Publication 2015
Bicarbonate, Sodium Brain Cells Cysteine Deoxyribonucleases Diagnosis Epidermolysis Bullosa Simplex Superficialis G 130 Glucose Ovomucin Papain Phosphates Saline Solution Salts Tissues Trypan Blue Trypsin Inhibitors
3
Quantitative Analysis of Carotenoids in Tomato
Cited 9 times
All solvents and NaCl were obtained from Fisher Scientific (Pittsburgh, PA, USA). Acetone and methyl tert-butyl ether (MTBE) were HPLC grade and methanol and water were Optima grade. Ammonium acetate was purchased from J.T. Baker (Phillipsburg, NJ, USA). Lycopene was isolated and crystallized from tomato paste as previously described [25 (link)]. Phytoene, phytofluene, ζ-carotene, neurosporene and tetra-cis-lycopene were isolated from tangerine tomato extracts using preparative HPLC. Identity and purity (>95%) was confirmed with HPLC/accurate mass before using as an external calibrant.
Carotenoids from tomato juices were analyzed using HPLC-DAD (Alliance 2695, 996 DAD, Waters Corporation, Milford, MA, USA) and TRL extracts were analyzed using HPLC-DAD-MS/MS (Agilent 1260, Santa Clara, CA, interfaced with an AB Sciex QTrap 5500 mass spectrometer, Foster City, CA, USA). Analytes were separated on a C30 column (4.6×250 mm, 3 μm, YMC Inc., Wilmington, NC, USA) at 35 °C using a gradient of A: 60% methanol, 35% MTBE, 3% water, 2% aqueous ammonium acetate (2% w/v), and B: 78% MTBE, 20% methanol, 2% aqueous ammonium acetate (2% w/v) flowing at 1.3 mL/min. A linear gradient was applied as follows: 0% B to 35.6% B over 9 min, to 100% B over the next 6.5 min, hold for 3.5 min at 100% B, and equilibrate for 3.5 min at initial conditions. Tomato juice extracts were re-dissolved in 2 mL of 1:1 MTBE:methanol, filtered using a 13 mm, 0.2 μm pore nylon filter, and 10 μL was injected. TRL extracts were re-dissolved in 200 μL 1:1 MTBE:methanol, centrifuged (model 5424, Eppendorf, Hamburg, Germany) at 21,130 × g for 2 min, and 20 μL of the supernatant was injected. Phytoene, phytofluene and ζ-carotene were quantified using DAD while neurosporene and all lycopene isomers were quantified using MS/MS. HPLC-DAD-MS/MS parameters are shown in Table 2.
Cooperstone J.L., Ralston R.A., Riedl K.M., Haufe T.C., Schweiggert R.M., King S.A., Timmers C.D., Francis D.M., Lesinski G.B., Clinton S.K, & Schwartz S.J. (2015). Enhanced bioavailability of lycopene when consumed as cis-isomers from tangerine compared to red tomato juice, a randomized, cross-over clinical trial. Molecular nutrition & food research, 59(4), 658-669.
Publication 2015
Acetone ammonium acetate Carotene Carotenoids Citrus reticulata G 130 High-Performance Liquid Chromatographies Isomerism Lycopene Methanol methyl tert-butyl ether neurosporene Nylons Paste phytoene, (15-cis)-isomer phytofluene Sodium Chloride Solvents Tandem Mass Spectrometry Tetragonopterus Tomatoes
4
Metabolic Syndrome in Chinese Children
Cited 8 times
Overweight was defined as a BMI ≥85th percentile and <95th percentile for gender and age, and obesity was defined as a BMI greater than or equal to the gender- and age-specific 95th percentile according to the Chinese BMI classification for children [25 ]. No universally-accepted threshold defines hyperuricemia in children; in this study, we defined hyperuricemia using the threshold of a UA value ≥357 μmol/L in accordance with previous studies [26 (link)]. Anaemia was defined according to the WHO criteria as a Hb <115 g/L for children aged ≥5 and <12 years, <120 g/L for children aged ≥12 and <15 years, <120 g/L for girls aged ≥15 years, and <130 g/L for boys aged ≥15 years [27 ]. Insulin resistance (IR) is affected by age and pubertal status [28 (link)], but no Tanner stage data was available for all participants in the database. To assess the age-related associations of MetS and IR, all children were divided into three age groups (7–10, 11–13, and 14–18 for girls; 7–11, 12–14, and 15–18 for boys) to reflect the prepubertal, pubertal, and postpubertal stages, respectively, according to the Chinese classification [29 (link),30 (link),31 (link)]. Currently, no universal definition of IR is applicable in normal and overweight children, so we adopted the 75th percentile of the homeostasis model assessment (HOMA: fasting serum insulin (μU/mL) × fasting plasma glucose (mmol/L)/22.5) within each age group as the threshold of IR [5 (link),32 (link)]. The IR thresholds assessed by the HOMA index are listed in Table 1.
In this study, MetS and its components in children aged 7–18 years were defined according to the modified criteria of the NCEP-ATP III [6 (link)]. MetS was identified when three or more of the following five components were present: (1) abdominal obesity: a WC equal to or above the gender- and age-specific 90th percentile for Chinese children [33 (link)]; (2) elevated TG: a TG ≥110 mg/dL; (3) low HDL: a HDL ≤40 mg/dL; (4) elevated blood pressure: an SBP and/or a DBP ≥90th percentile for gender, age, and height [24 (link)]; (5) elevated fasting glucose: a glucose ≥110 mg/dL. Moreover, the IDF definition was also applied to explore the concordance with the NCEP-ATP III definition in children aged 10–18 years [8 (link)].
Song P., Yu J., Chang X., Wang M, & An L. (2017). Prevalence and Correlates of Metabolic Syndrome in Chinese Children: The China Health and Nutrition Survey. Nutrients, 9(1), 79.
Full text: Click here
Publication 2017
Age Groups Anemia Blood Pressure Boys Child Chinese G 130 Glucose Homeostasis Hyperuricemia Insulin Insulin Resistance Obesity Plasma Puberty Serum Woman
5
Genome assembly of Drosophila melanogaster
Cited 7 times
The genomes were assembled following the approach described in Chakraborty et al.22 (link). For all calculations of sequence coverage, a genome size of 130Mbp is assumed (G = 130 × 106 bp). For individual strain, we generated a hybrid assembly with DBG2OLC65 (link) and longest 30X PacBio reads, and a PacBio assembly with canu v1.366 (link) (Supplementary Data 3). The paired end Illumina reads were obtained from King et al.24 (link). The hybrid assemblies were merged with the PacBio only assemblies with quickmerge v0.222 (link),67 (l = 2 Mb, ml = 20000, hco = 5.0, c = 1.5), with the hybrid assembly being used as the query. Because the PacBio assembly sizes were closer to the genome size of D. melanogaster, we added the contigs that were present only in the PacBio only assembly but not the hybrid assembly by performing a second round of quickmerge67 . For the second round of quickmerge (l = 5 mb, ml = 20000, hco = 5.0, c = 1.5), the PacBio assembly was used as the query and the merged assembly from the first merging round the reference assembly. The resulting merged assembly was processed with finisherSC to remove the redundant sequences and additional gap filling using raw reads68 (link). The assemblies were then polished twice with quiver (SMRTanalysis v2.3.0p5) and once with Pilon v1.1669 (link) using the same Illumina reads as used for the hybrid assemblies.
Chakraborty M., Emerson J.J., Macdonald S.J, & Long A.D. (2019). Structural variants exhibit widespread allelic heterogeneity and shape variation in complex traits. Nature Communications, 10, 4872.
Full text: Click here
Publication 2019
G 130 Genome Hybrids Strains Tremor

Most recents protocols related to «G 130»

1
Synthesis and Characterization of Porous Chromatography Particles
Not available on PMC !

Example 1

<Step (A): Synthesis of porous particle having glycidyl group>

27.8 g of glycidyl methacrylate (trade name: Blemmer G (registered trademark) manufactured by NOF Corporation), 11.3 g of glycerin-1,3-dimethacrylate (trade name: NK Ester 701, SHIN-NAKAMURA CHEMICAL Co., Ltd.), and 1.9 g of 2,2′-azobis(2,4-dimethylvaleronitrile) were dissolved in 58.7 g of diethyl succinate as a diluent, and nitrogen gas was bubbled for 30 minutes to provide an oil phase.

Next, separately from the oil phase, 10.0 g of PVA-224 (manufactured by Kuraray Co., Ltd., polyvinyl alcohol having a degree of saponification of 87.0% to 89.0%) as a dispersion stabilizer and 10.0 g of sodium chloride as a salting-out agent were dissolved in 480 g of ion exchanged water to provide an aqueous phase.

The aqueous phase and the oil phase were placed in a separable flask and dispersed at a rotation speed of 430 rpm for 20 minutes using a stirring rod equipped with a half-moon stirring blade, then the inside of the reactor was purged with nitrogen, and the reaction was carried out at 60° C. for 16 hours.

After that, the resulting polymer was transferred onto a glass filter and thoroughly washed with hot water at about 50 to 80° C., denatured alcohol, and water in the order presented to obtain 100.4 g of a porous particle (carrier al).

The amount of glycidyl methacrylate used was 79.8 mol % based on the total amount of the monomers, and the amount of glycerin-1,3-dimethacrylate used was 20.2 mol % based on the total amount of the monomers.

<Step (B): Introduction reaction of alkylene group>

98 g of the carrier α1 was weighed onto a glass filter and thoroughly cleaned with diethylene glycol dimethyl ether. After cleaning, the carrier α1 was placed in a 1 L separable flask, 150 g of diethylene glycol dimethyl ether and 150 g (920 mol % based on glycidyl methacrylate) of 1,4-butanediol were placed in the separable flask, and stirring and dispersion were carried out.

After that, 1.5 ml of a boron trifluoride diethyl ether complex was added, the temperature was raised to 80° C. while stirring at 200 rpm, and the resulting mixture was subjected to the reaction for 4 hours.

The mixture was cooled, then the porous particle (carrier β1) bonded to a diol compound including an alkylene group in the structure thereof was collected by filtration and then washed with 1 L of ion exchanged water to obtain 152 g of a carrier β1.

The progress of the reaction was confirmed by the following procedure.

A part of the dry porous particle into which an alkylene group had been introduced was mixed with potassium bromide, and the resulting mixture was pelletized by applying a pressure and then measured using FT-IR (trade name: Nicolet (registered trademark) iS10, manufactured by Thermo Fisher Scientific Inc.) to check the height of an absorbance peak at 908 cm−1 due to the glycidyl group in the infrared absorption spectrum.

As a result, no absorbance peak at 908 cm−1 was observed by FT-IR.

<Step (C): Introduction Reaction of Glycidyl Group>

150 g of the carrier β1 was weighed onto a glass filter and thoroughly cleaned with dimethylsulfoxide.

After cleaning, the carrier β1 was placed in a separable flask, 262.5 g of dimethyl sulfoxide and 150 g of epichlorohydrin were added, the resulting mixture was stirred at room temperature, 37.5 ml of a 30% sodium hydroxide aqueous solution (manufactured by KANTO CHEMICAL CO., INC.) was further added, and the resulting mixture was heated to 30° C. and stirred for 6 hours.

After completion of the reaction, the obtained product was transferred onto a glass filter and thoroughly washed with water, acetone, and water in the order presented to obtain 172 g of a porous particle into which a glycidyl group had been introduced (carrier γ1).

The introduction density of the glycidyl group in the obtained carrier γ1 was measured by the following procedure.

5.0 g of the carrier γ1 was sampled, and the dry mass thereof was measured and as a result, found to be 1.47 g. Next, the same amount of the carrier γ1 was weighed into a separable flask and dispersed in 40 g of water, 16 mL of diethylamine was added while stirring at room temperature, and the resulting mixture was heated to 50° C. and stirred for 4 hours. After completion of the reaction, the reaction product was transferred onto a glass filter and thoroughly washed with water to obtain a porous particle A into which diethylamine had been introduced.

The obtained porous particle A was transferred into a beaker and dispersed in 150 mL of a 0.5 mol/L potassium chloride aqueous solution, and titration was carried out using 0.1 mol/L hydrochloric acid with the point at which the pH reached 4.0 as the neutralization point.

From this, the amount of diethylamine introduced into the porous particle A into which diethylamine had been introduced was calculated, and the density of the glycidyl group of the carrier γ1 was calculated from the following expression.

As a result, the density of the glycidyl group was 880 μmol/g.
Density(μmol/g) of glycidyl group={0.1×volume(μL) of hydrochloric acid at neutralization point/dry mass(g) of porous particle into which glycidyl group has been introduced}<Step (D): Introduction Reaction of Polyol>

150 g of the carrier γ1, 600 mL of water, and 1000 g (13000 mol % based on glycidyl group) of D-sorbitol (log P=−2.20, manufactured by KANTO CHEMICAL CO., INC.) were placed in a 3 L separable flask and stirred to form a dispersion.

After that, 10 g of potassium hydroxide was added, the temperature was raised to 60° C. while stirring at 200 rpm, and the resulting mixture was subjected to the reaction for 15 hours.

The mixture was cooled, and then the reaction product was collected by filtration and washed thoroughly with water to obtain 152 g of a porous particle into which polyol had been introduced (carrier 61).

The obtained carrier 61 was classified into 16 to 37 μm using a sieve to obtain 140.5 g of a packing material 1.

<Evaluation of Alkali Resistance>

The alkali resistance was evaluated by calculating the amount of a carboxy group produced by hydrolysis of sodium hydroxide according to the following procedure.

First, 4 g of the packing material was dispersed in 150 mL of a 0.5 mol/L potassium chloride aqueous solution, and titration was carried out using 0.1 mol/L sodium hydroxide aqueous solution with the point at which the pH reached 7.0 as the neutralization point. From this, the amount of a carboxy group before hydrolysis included in the packing material was calculated from the following expression.
Amount(μmol/mL) of carboxy group=0.1×volume(μL) of sodium hydroxide aqueous solution at the time of neutralization/apparent volume (mL) of packing material

Here, the apparent volume of the packing material is the volume of the packing material phase measured after preparing a slurry liquid by dispersing 4 g of the packing material in water, transferring the slurry liquid to a graduated cylinder, and then allowing the same to stand for a sufficient time.

Subsequently, 4 g of the packing material was weighed into a separable flask, 20 mL of a 5 mol/L sodium hydroxide aqueous solution was added, and the resulting mixture was treated at 50° C. for 20 hours while stirring at 200 rpm. The mixture was cooled, then the packing material was collected by filtration, then washed with a 0.1 mol/L HCl aqueous solution and water in the order presented, and the amount of a carboxy group contained in the obtained packing material was calculated by the same method as above. From the difference between the amount of a carboxy group before and that after the reaction with the 5 mol/L sodium hydroxide aqueous solution, the amount of a carboxy group produced by the reaction with the 5 mol/L sodium hydroxide aqueous solution was calculated. As a result, the amount of a carboxy group produced was 21 μmol/mL.

If the amount of a carboxy group produced is 40 μmol/mL or less, the alkali resistance is considered to be high.

<Evaluation of Non-Specific Adsorption>

The obtained packing material was packed into a stainless steel column (manufactured by Sugiyama Shoji Co., Ltd.) having an inner diameter of 8 mm and a length of 300 mm by a balanced slurry method. Using the obtained column, a non-specific adsorption test was carried out by the method shown below.

The column packed with the packing material was connected to a Shimadzu Corporation HPLC system (liquid feed pump (trade name: LC-10AT, manufactured by Shimadzu Corporation), autosampler (trade name: SIL-10AF, manufactured by Shimadzu Corporation), and photodiode array detector (trade name: SPD-M10A, manufactured by Shimadzu Corporation)), and a 50 mmol/L sodium phosphate buffer aqueous solution as a mobile phase was passed at a flow rate of 0.6 mL/min.

Using the same sodium phosphate aqueous solution as the mobile phase as a solvent, their respective sample solutions of 0.7 mg/mL thyroglobulin (Mw of 6.7×105), 0.6 mg/mL γ-globulin (Mw of 1.6×105), 0.96 mg/mL BSA (Mw of 6.65×104), 0.7 mg/mL ribonuclease (Mw of 1.3×104), 0.4 mg/mL aprotinin (Mw of 6.5×103), and 0.02 mg/mL uridine (Mw of 244) (all manufactured by Merck Sigma-Aldrich) are prepared, and 10 μL of each is injected from the autosampler.

The elution time of each observed using the photodiode array detector at a wavelength of 280 nm was compared to confirm that there was no contradiction between the order of elution volume and the order of molecular weight size.

As a result, the elution volumes of the samples from the column packed with the packing material 1 were 8.713 mL, 9.691 mL, 9.743 mL, 10.396 mL, 11.053 mL, and 11.645 mL, and it was confirmed that there was no contradiction between the order of the molecular weights of the samples and the order of the elution volumes thereof and that no non-specific adsorption was induced. When there was no contradiction between the order of the molecular weights of the samples and the order of the elution volumes thereof, there was no non-specific adsorption, which is indicated as 0 in Table 1, and when there was a contradiction therebetween, non-specific adsorption was induced, which is thus indicated as X.

The porous particle (carrier al) obtained in the same manner as in Example 1 was subjected to the step D of Example 1.

<Step (D): Introduction Reaction of Polyol>

98 g of carrier al, 600 mL of water, and 1000 g (3050 mol % based on glycidyl group) of D-sorbitol (manufactured by KANTO CHEMICAL CO., INC.) were placed in a 3 L separable flask and stirred to form a dispersion.

After that, 10 g of potassium hydroxide was added, the temperature was raised to 60° C. while stirring at 200 rpm, and the resulting mixture was subjected to the reaction for 15 hours.

The mixture was cooled, and then the reaction product was collected by filtration and washed thoroughly with water to obtain 130 g of a porous particle into which a polyol had been introduced (carrier δ7).

The carrier δ7 was classified into 16 to 37 μm using a sieve to obtain 115 g of a packing material 7.

The alkali resistance of the obtained packing material 7 was evaluated in the same manner as in Example 1. As a result, the amount of a carboxy group produced in the packing material 7 was 120.3 μmol/mL, resulting in poor alkali resistance.

Further, the non-specific adsorption of the obtained packing material 7 was evaluated in the same manner as in Example 1. As a result, the elution volumes of the samples were 8.606 mL, 9.769 mL, 9.9567 mL, 10.703 mL, 11.470 mL, and 12.112 mL, and it was confirmed that there was no contradiction between the order of the molecular weights of the samples and the order of the elution volumes thereof and that no non-specific adsorption was induced.

Example 2

A porous particle (carrier al) was obtained in the same manner as in Example 1, and then a packing material 2 was obtained as follows.

98 g of the carrier α1 was weighed onto a glass filter and thoroughly cleaned with diethylene glycol dimethyl ether.

After cleaning, the porous particle was placed in a 1 L separable flask, 150 g of diethylene glycol dimethyl ether and 150 g (580 mol % based on the glycidyl group) of 1,4-cyclohexanedimethanol were placed in the separable flask, and stirring and dispersion were carried out.

After that, 1.5 ml of a boron trifluoride diethyl ether complex was added, the temperature was raised to 80° C. while stirring at 200 rpm, and the resulting mixture was subjected to the reaction for 4 hours.

The mixture was cooled, then the resulting porous particle (carrier $2) bonded to a diol compound including an alkylene group in the structure thereof was collected by filtration and then washed with 1 L of ion exchanged water to obtain 165 g of a carrier 32.

The progress of the reaction was confirmed by the following procedure.

A part of the dry porous particle into which an alkylene group had been introduced was mixed with potassium bromide, and the resulting mixture was pelletized by applying a pressure and then measured using FT-IR (trade name: Nicolet (registered trademark) iS10, manufactured by Thermo Fisher Scientific Inc.) to check the height of a absorbance peak at 908 cm−1 due to the glycidyl group in the infrared absorption spectrum.

As a result, no absorbance peak at 908 cm−1 was observed by FT-IR.

<Step (C): Introduction Reaction of Glycidyl Group>

150 g of the carrier $2 was weighed onto a glass filter and thoroughly cleaned with dimethylsulfoxide. After cleaning, the carrier $2 was placed in a separable flask, 262.5 g of dimethyl sulfoxide and 150 g of epichlorohydrin were added, the resulting mixture was stirred at room temperature, 37.5 ml of a 30% sodium hydroxide aqueous solution (manufactured by KANTO CHEMICAL CO., INC.) was further added, and the resulting mixture was heated to 30° C. and stirred for 6 hours. After completion of the reaction, the porous particle was transferred onto a glass filter and thoroughly washed with water, acetone, and water in the order presented to obtain 180 g of a porous particle into which a glycidyl group had been introduced (carrier γ2).

The introduction density of the glycidyl group in the obtained carrier γ2 was measured in the same manner as in Example 1. As a result, the density of the glycidyl group was 900 μmol/g.

<Step (D): Introduction Reaction of Polyol>

150 g of the carrier γ2 was weighed onto a glass filter and thoroughly cleaned with diethylene glycol dimethyl ether. After cleaning, the carrier γ2 was placed in a 1 L separable flask, 150 g of diethylene glycol dimethyl ether and 150 g (5760 mol % based on the glycidyl group) of ethylene glycol (log P=−1.36) were placed in the separable flask, and stirring and dispersion were carried out. After that, 1.5 mL of a boron trifluoride diethyl ether complex was added, the temperature was raised to 80° C. while stirring at 200 rpm, and the resulting mixture was subjected to the reaction for 4 hours. The mixture was cooled, and then the reaction product was collected by filtration and washed thoroughly with water to obtain 152 g of a polyol-introduced porous particle (carrier δ2). The carrier δ2 was classified into 16 to 37 μm using a sieve to obtain 140.5 g of a packing material 2.

The alkali resistance of the obtained packing material 2 was evaluated in the same manner as in Example 1. As a result, the amount of a carboxy group produced was 15.2 μmol/mL, and it was confirmed that the packing material 2 had excellent alkali resistance.

Further, the non-specific adsorption of the obtained packing material 2 was evaluated in the same manner as in Example 1. As a result, the elution volumes of the samples were 8.814 mL, 9.635 mL, 9.778 mL, 10.37 mL, 10.898 mL, and 12.347 mL, and it was confirmed that there was no contradiction between the order of the molecular weights of the samples and the order of the elution volumes thereof and that no non-specific adsorption was induced.

A packing material 8 was obtained in the same manner as in Example 1 except that 150 g of ethylene glycol was used instead of 1,4-butanediol as an alkylene group-introducing agent.

The alkali resistance of the obtained packing material 8 was evaluated in the same manner as in Example 1. As a result, the amount of a carboxy group produced in the packing material 8 was 108.4 μmol/mL, resulting in poor alkali resistance.

Further, the non-specific adsorption of the obtained packing material 8 was evaluated in the same manner as in Example 1. As a result, the elution volumes of the samples were 9.708 mL, 9.8946 mL, 10.6452 mL, 11.5374 mL, and 12.1656 mL, and it was confirmed that there was no contradiction between the order of the molecular weights of the samples and the order of the elution volumes thereof and that no non-specific adsorption was induced.

Example 3

A carrier γ2 was obtained in the same manner as in Example 2.

150 g of the obtained carrier γ2 was weighed onto a glass filter and thoroughly cleaned with diethylene glycol dimethyl ether.

After cleaning, the porous particle was placed in a 1 L separable flask, 150 g of diethylene glycol dimethyl ether and 150 g of polyethylene glycol #200 (manufactured by KANTO CHEMICAL CO., INC., average molecular weight of 190 to 210, log P is unclear, but the close compound tetraethylene glycol (Mw of 194) has a log P of −2.02) (1790 mol % based on glycidyl group) were placed in the separable flask, and stirring and dispersion were carried out.

After that, 1.5 mL of a boron trifluoride diethyl ether complex was added, the temperature was raised to 80° C. while stirring at 200 rpm, and the resulting mixture was subjected to the reaction for 4 hours.

The mixture was cooled, and then the reaction product was collected by filtration and washed thoroughly with water to obtain 152 g of a porous particle into which a polyol had been introduced (carrier 63).

The carrier δ3 was classified into 16 to 37 μm using a sieve to obtain 140.5 g of a packing material 3.

The alkali resistance of the obtained packing material 3 was evaluated in the same manner as in Example 1. As a result, the amount of a carboxy group produced was 16.1 μmol/mL, and it was confirmed that the packing material 3 had excellent alkali resistance.

Further, the non-specific adsorption of the obtained packing material 3 was evaluated in the same manner as in Example 1. As a result, the elution volumes of the samples were 8.517 mL, 9.241 mL, 9.47 mL, 10.034 mL, 10.484 mL, and 11.927 mL, and it was confirmed that there was no contradiction between the order of the molecular weights of the samples and the order of the elution volumes thereof and that no non-specific adsorption was induced.

A packing material 9 was obtained in the same manner as in Example 2 except that no glycidyl group was introduced and no polyol was introduced. That is, the carrier $2 obtained in the step (B) of Example 2 was used as the packing material 9.

The non-specific adsorption of the obtained packing material 9 was evaluated in the same manner as in Example 1. As a result, the elution volumes of the samples were 8.590 mL, 10.316 mL, 9.603 mL, 10.484 mL, 13.863 mL, and 12.861 mL, and it was confirmed that there was a contradiction between the order of the molecular weights of the samples and the order of the elution volumes thereof and that non-specific adsorption was induced. Because of this, the alkali resistance was not evaluated.

Example 4

A packing material 4 was obtained in the same manner as in Example 3 except that 33.2 g of glycidyl methacrylate (trade name: Blemmer G (registered trademark) manufactured by NOF Corporation), 5.9 g of glycerin-1,3-dimethacrylate (trade name: NK Ester 701, SHIN-NAKAMURA CHEMICAL Co., Ltd.), 58.7 g of diethyl succinate, and 1.9 g of 2,2′-azobis(2,4-dimethylvaleronitrile) were used to provide an oil phase. The amount of glycidyl methacrylate used was 90.0 mol % based on the total amount of the monomers, and the amount of glycerin-1,3-dimethacrylate used was 10.0 mol % based on the total amount of the monomers.

The alkali resistance of the obtained packing material 4 was evaluated in the same manner as in Example 1. As a result, the amount of a carboxy group produced was 11.5 μmol/mL, and it was confirmed that the packing material 4 had excellent alkali resistance.

Further, the non-specific adsorption of the obtained packing material 4 was evaluated in the same manner as in Example 1. As a result, the elution volumes of the samples were 7.52 mL, 8.214 mL, 8.451 mL, 9.062 mL, 9.511 mL, and 11.915 mL, and it was confirmed that there was no contradiction between the order of the molecular weights of the samples and the order of the elution volumes thereof and that no non-specific adsorption was induced.

A packing material 10 was obtained in the same manner as in Example 1 except that 150 g (480 mol % based on glycidyl methacrylate) of 1,10-decanediol was used instead of 1,4-butanediol as an alkylene group-introducing agent.

The non-specific adsorption of the obtained packing material 10 was evaluated in the same manner as in Example 1. As a result, the elution volumes of the samples were 9.991 mL, 10.15 mL, 10.063 mL, 10.691 mL, 12.172 mL, and 11.531 mL, and it was confirmed that there was a contradiction between the order of the molecular weights of the samples and the order of the elution volumes thereof and that non-specific adsorption was induced. Because of this, the alkali resistance was not evaluated.

Example 5

A packing material 5 was obtained in the same manner as in Example 3 except that 21.5 g of glycidyl methacrylate (trade name: Blemmer G (registered trademark) manufactured by NOF Corporation), 17.6 g of glycerin-1,3-dimethacrylate (trade name: NK Ester 701, SHIN-NAKAMURA CHEMICAL Co., Ltd.), 58.7 g of diethyl succinate, and 1.9 g of 2,2′-azobis(2,4-dimethylvaleronitrile) were used to provide an oil phase.

The amount of glycidyl methacrylate used was 66.2 mol % based on the total amount of the monomers, and the amount of glycerin-1,3-dimethacrylate used was 33.8 mol % based on the total amount of the monomers.

The alkali resistance of the obtained packing material 5 was evaluated in the same manner as in Example 1. As a result, the amount of a carboxy group produced was 18.3 μmol/mL, and it was confirmed that the packing material 5 had excellent alkali resistance.

Further, the non-specific adsorption of the obtained packing material 5 was evaluated in the same manner as in Example 1. As a result, the elution volumes of the samples were 8.692 mL, 9.434 mL, 9.625 mL, 10.236 mL, 10.759 mL, and 12.457 mL, and it was confirmed that there was no contradiction between the order of the molecular weights of the samples and the order of the elution volumes thereof and that no non-specific adsorption was induced.

A packing material 11 was obtained in the same manner as in Example 3 except that 13.7 g of glycidyl methacrylate (trade name: Blemmer G (registered trademark) manufactured by NOF Corporation), 25.4 g of glycerin-1,3-dimethacrylate (trade name: NK Ester 701, SHIN-NAKAMURA CHEMICAL Co., Ltd.), 58.7 g of diethyl succinate, and 1.9 g of 2,2′-azobis(2,4-dimethylvaleronitrile) were used to provide an oil phase. The amount of glycidyl methacrylate used was 46.4 mol % based on the total amount of the monomers, and the amount of glycerin-1,3-dimethacrylate used was 53.6 mol % based on the total amount of the monomers.

The non-specific adsorption of the obtained packing material 11 was evaluated in the same manner as in Example 1. As a result, the elution volumes of the samples were 8.872 mL, 10.131 mL, 9.82 mL, 10.422 mL, 12.782 mL, and 12.553 mL, and it was confirmed that there was a contradiction between the order of the molecular weights of the samples and the order of the elution volumes thereof and that non-specific adsorption was induced. Because of this, the alkali resistance was not evaluated.

It was confirmed that the exclusion limit molecular weights of the packing materials obtained in Examples 1 to 6 and Comparative Examples 1 to 5 were all 1,000,000 or more.

Example 6

A packing material 6 was obtained in the same manner as in Example 3 except that 33.2 g of glycidyl methacrylate (trade name: Blemmer G (registered trademark) manufactured by NOF Corporation), 5.9 g of ethylene glycol dimethacrylate (trade name: NK Ester 1G, SHIN-NAKAMURA CHEMICAL Co., Ltd.), 29.3 g of butyl acetate, 29.3 g of chlorobenzene, and 1.9 g of 2,2′-azobis(2,4-dimethylvaleronitrile) were used to provide an oil phase. The amount of glycidyl methacrylate used was 88.7 mol % based on the total amount of the monomers, and the amount of ethylene glycol dimethacrylate used was 11.3 mol % based on the total amount of the monomers.

The alkali resistance of the obtained packing material 6 was evaluated in the same manner as in Example 1. As a result, the amount of a carboxy group produced was 12.5 μmol/mL, and it was confirmed that the packing material 6 had excellent alkali resistance.

Further, the non-specific adsorption of the obtained packing material 6 was evaluated in the same manner as in Example 1. As a result, the elution volumes of the samples were 9.613 mL, 10.427 mL, 10.444 mL, 11.066 mL, 11.582 mL, and 12.575 mL, and it was confirmed that there was no contradiction between the order of the molecular weights of the samples and the order of the elution volumes thereof and that no non-specific adsorption was induced.

A packing material 12 was obtained in the same manner as in Example 3 except that 37.1 g of glycidyl methacrylate (trade name: Blemmer G (registered trademark) manufactured by NOF Corporation), 2.0 g of glycerin-1,3-dimethacrylate (trade name: NK Ester 701, SHIN-NAKAMURA CHEMICAL Co., Ltd.), 58.7 g of diethyl succinate, and 1.9 g of 2,2′-azobis(2,4-dimethylvaleronitrile) were used to provide an oil phase. The amount of glycidyl methacrylate used was 96.7 mol % based on the total amount of the monomers, and the amount of glycerin-1,3-dimethacrylate used was 3.3 mol % based on the total amount of the monomers.

Packing into a stainless steel column using the obtained packing material 12 was attempted. However, the back pressure was high, making liquid feeding difficult, and this made it impossible to carry out the packing. Because of this, neither of the evaluations was able to be carried out.

Results of the above Examples and Comparative Examples are shown in Table 1.

From the above results, by adopting the configuration of the present invention, a packing material having suppressed non-specific adsorption and high alkali resistance can be obtained.

When no hydrophobic portion is provided or when the alkylene chain is short, the alkali resistance is low as shown in Comparative Examples 1 and 2. In addition, it was found that when the alkylene chain is too long or when no hydrophilic portion is provided, the hydrophobicity is strong, and non-specific adsorption is induced as shown in Comparative Examples 3 and 4. In addition, in Comparative Example 5 having many repeating units derived from a polyfunctional monomer, it was found that non-specific adsorption was induced, and in Comparative Example 6 having fewer repeating units derived from a polyfunctional monomer, it was found that the back pressure applied to the apparatus was high, making column packing difficult.

TABLE 1
Amount of
carboxy
Degree ofgroup
PolyfunctionalcrosslinkingNon-specificproduced
Monomer[mol %]Alkylene groupPolyoladsorption5)[μmol/mL]
Ex. 1GDMA1)20.2Butylene groupSorbitol◯21
Ex. 2GDMA20.2Cyclohexane-1,4-dimethyleneEG3)◯15.2
group
Ex. 3GDMA20.2Cyclohexane-1,4-dimethylenePEG2004)◯16.1
group
Ex. 4GDMA10Cyclohexane-1,4-dimethylenePEG200◯11.5
group
Ex. 5GDMA33.8Cyclohexane-1,4-dimethylenePEG200◯18.3
group
Ex. 6EDMA2)11.3Cyclohexane-1,4-dimethylenePEG200◯12.5
group
Comp.GDMA20.2—Sorbitol◯120.3
Ex. 1
Comp.GDMA20.2Ethylene groupEG◯108.4
Ex. 2
Comp.GDMA20.2Cyclohexane-1,4-dimethylene—X—
Ex. 3group
Comp.GDMA20.2Decanylene groupSorbitolX—
Ex. 4
Comp.GDMA53.6Cyclohexane-1,4-dimethylenePEG200X—
Ex. 5group
Comp.GDMA3.3Cyclohexane-1,4-dimethylenePEG200Unmeasurable
Ex. 6group
1)GDMA: Glycerin-1,3-dimethacrylate
2)EDMA: Ethylene glycol dimethacrylate
3)EG: Ethylene glycol
4)PEG200: Polyethylene glycol #200
5)◯: No non-specific adsorption, X: Non-specific adsorption

US11857949B2. Packing material and method for producing the same, and column for size exclusion chromatography (2024-01-02). Resonac Corporation [JP]. Inventors: Naoki Uchiyama [JP], Yuzuru Kokido [JP].
Full text: Click here
Patent 2024
A 300 Acetone Adsorption Alkalies Anabolism Aprotinin boron trifluoride Buffers butyl acetate butylene Butylene Glycols chlorobenzene COMP protocol Cyclohexane cyclohexanedimethanol diethylamine diethyl succinate diglyme Epichlorohydrin Esters Ethanol ethylene dimethacrylate Ethylenes Ethyl Ether Filtration G 130 gamma-Globulin Gel Chromatography Glycerin glycidyl methacrylate Glycol, Ethylene High-Performance Liquid Chromatographies Hydrochloric acid Hydrolysis Nitrogen Polyethylene Glycols Polymers polyol Polyvinyl Alcohol potassium bromide Potassium Chloride potassium hydroxide Pressure Ribonucleases Sodium Hydroxide sodium phosphate Solvents Sorbitol Stainless Steel Sulfoxide, Dimethyl tetraethylene glycol Thyroglobulin Titrimetry Uridine
2
Hemoglobin Criteria for Anemia
Anemia was described as a hemoglobin concentration < 120 g/L for women and < 130 g/L for men (23 (link)).
Huang J., Xu J., Ye P, & Xin X. (2023). Association between magnesium intake and the risk of anemia among adults in the United States. Frontiers in Nutrition, 10, 1046749.
Full text: Click here
Publication 2023
Anemia G 130 Hemoglobin A Woman
3
Anemia, Myocardial Infarction, and Bleeding Outcomes
Anemia was defined according to World Health Organization recommendations: a serum hemoglobin level of <130 g/L for men and <120 g/L for women (24 ). The hemoglobin level was determined at admission. The MI was confirmed in patients by a history of chest pain, diagnostic electrocardiographic changes, and serial elevations of cardiac biomarkers (above the 99th percentile URL in our laboratory) according to published guidelines (25 –27 (link)). The Bleeding Academic Research Consortium (BARC) 3a bleeding criteria were used (28 (link)). Renal dysfunction was defined as a glomerular filtration rate (GFR) of less than 60 ml/kg/1.73 m2. The ventricular ejection fraction was assessed by bedside echocardiography in the first 48 h after admission. Heart failure was defined according to clinical criteria (bilateral pulmonary rales, S3 gallop, edema) and/or pulmonary edema on chest X-ray and/or ejection fraction <30%. The diagnosis of cardiogenic shock (CS) was made based on the accepted definition of a systolic blood pressure ≤90 mm Hg for ≥30 min or the need for supportive measures to maintain a systolic blood pressure of >90 mm Hg, clinical signs of pulmonary congestion, and signs of end-organ hypoperfusion.
Kanic V., Kompara G, & Suran D. (2023). Differential impact of anemia in relation to sex in patients with myocardial infarction. Frontiers in Cardiovascular Medicine, 10, 1108710.
Full text: Click here
Publication 2023
Anemia Biological Markers Chest Pain Diagnosis Echocardiography Edema Electrocardiography G 130 Glomerular Filtration Rate Heart Heart Failure Hemoglobin Hemoglobin A Kidney Failure Lung Patients Pulmonary Edema Radiography, Thoracic Serum Shock, Cardiogenic Systolic Pressure Ventricular Ejection Fraction Woman
4
Automated Segmentation Workflow for Multiplexed IF
For mask generation, it is necessary to determine the cut-off values for positive IF signals and remove false-positive signals due to artifacts, registration errors, or non-specific signals from blood cells.
Inconsistencies between the intensities of the DAPI nuclear channel in the IF image and the hematoxylin component in the H&E-stained image, indicating the existence of artifacts or registration errors, were detected by calculating the Pearson’s correlation coefficient between the two signal intensities. Patches with correlation coefficients below 0.5 were removed for further analysis. False-positive signals derived from the autofluorescence of RBCs were removed by masking the positively predicted regions using the RBC segmentation neural network trained on the anti-CD235a antibody-stained dataset. Based on visual inspection, an IF signal intensity >50 (epithelium, smooth muscle, and RBCs) or 25 (others) was regarded as a positive signal in the initial mask generation step.
For the epithelium and smooth muscle, the positive signal area was used as a segmentation mask without modification. For RBCs, the area that was positive in the IF image and red in the H&E-stained image (R > 100 and G < 130, and R > B) was used as a segmentation mask. For leukocytes, myeloid cells, lymphocytes, plasma cells, and endothelial cells, positive signals from the target cells were transferred into the nuclei based on the IF staining pattern to obtain a more consistent result and improve the interpretability of the segmentation model. Cellpose version 0.6.519 (link) was applied to the DAPI nuclear channel in the IF images to detect the nuclei. We selected a model with the following parameters: diameter = 30, channels = [3,0], batch_size = 64, and cellprob_threshold = 0.1. Nuclei were masked if over 40% of them contained positive signals. Finally, one iteration of morphological erosion with a 3 × 3 kernel was applied to each region of the nuclei to prevent multiple cells from sticking together, which could cause an underestimation of the cell count.
For deep neural network training during the mask generation process, all patches were divided into training, validation, or test sets so that all patches from the same TMA spot belonged to the same set. TMA spots in each TMA were detected as clusters by applying the DBSCAN clustering algorithm40 implemented in scikit-learn to patches using the x and y coordinates as the input features, maximum distance set to 3,000 pixels, and min_samples set to 5. The validation and test sets contained patches from two TMA spots in each TMA slide, and the rest were placed into the training set. For deep neural network training after mask generation, we moved the training/validation patches from the patient in the test set to the test set and training patches from the patients in the validation set to the validation set, so that the patches from the same patient did not span the training/validation/test sets.
Komura D., Onoyama T., Shinbo K., Odaka H., Hayakawa M., Ochi M., Herdiantoputri R.R., Endo H., Katoh H., Ikeda T., Ushiku T, & Ishikawa S. (2023). Restaining-based annotation for cancer histology segmentation to overcome annotation-related limitations among pathologists. Patterns, 4(2), 100688.
Full text: Click here
Publication 2023
Antibodies, Anti-Idiotypic BLOOD Cell Nucleus Cells Conditioning, Psychology DAPI Endothelial Cells Epithelium Erythrocytes Exanthema G 130 Glycophorin A Hydrogen Leukocytes Lymphocyte Myeloid Cells Patch Tests Patients Plasma Cells Signal Transduction Smooth Muscles
5
Anemia and Death Risk in PLWHA
According to the WHO diagnostic criteria, anemia is defined as a hemoglobin level of <130 g/L in males or < 120 g/L in females. To more comprehensively investigate the association of anemia with the death risk of PLWHA, anemia was further classified into two categories, in which hemoglobin from 110 to 129 g/L in males or 110 to 119 g/L in females was defined as mild anemia, and moderate or severe anemia was defined as hemoglobin <110 g/L (18 ).
Jin M., Wang Y., Li J., Wu Z., Liu X., Wang H., Chen Y., Wang Z., Tong Z., Li X., Ren F., Zhu X., Yang Z, & Mao G. (2023). Anemia is independently associated with mortality in people living with human immunodeficiency virus/acquired immune deficiency syndrome: A propensity score matching-based retrospective cohort study in China. Frontiers in Medicine, 10, 1055115.
Full text: Click here
Publication 2023
Anemia Diagnosis Females G 130 Hemoglobin Hemoglobin A Males

Top products related to «G 130»

1
Benzonase by Merck Group
Sourced in United States, Germany, Switzerland, United Kingdom, France, Denmark, Canada, Japan, Macao
Benzonase is a recombinant endonuclease enzyme that can degrade DNA and RNA. It is commonly used in various laboratory applications to remove nucleic acid contaminants from protein samples.
2
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
3
Protease inhibitor cocktail by Roche
Sourced in United States, Switzerland, Germany, China, United Kingdom, France, Canada, Japan, Italy, Australia, Austria, Sweden, Spain, Cameroon, India, Macao, Belgium, Israel
Protease inhibitor cocktail is a laboratory reagent used to inhibit the activity of proteases, which are enzymes that break down proteins. It is commonly used in protein extraction and purification procedures to prevent protein degradation.
4
Biorupter by Diagenode
Sourced in Belgium
The Biorupter is a laboratory equipment designed for the disruption and homogenization of biological samples. It utilizes high-frequency sound waves to break down cellular structures and release cellular contents. The Biorupter is a versatile tool that can be used in a variety of applications, including DNA, RNA, and protein extraction, as well as cell lysis and sample preparation for downstream analysis.
5
Pierce bca protein assay kit by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, Australia, Switzerland, France, Italy, Canada, Spain, Japan, Belgium, Sweden, Lithuania, Austria, Denmark, Poland, Ireland, Portugal, Finland, Czechia, Norway, Macao, India, Singapore
The Pierce BCA Protein Assay Kit is a colorimetric-based method for the quantification of total protein in a sample. It utilizes the bicinchoninic acid (BCA) reaction, where proteins reduce Cu2+ to Cu+ in an alkaline environment, and the resulting purple-colored reaction is measured spectrophotometrically.
6
Tissuelyser by Qiagen
Sourced in Germany, United States, United Kingdom, France, Switzerland, Netherlands, Canada, Spain, Italy, Japan, Denmark, Australia, Sweden
The TissueLyser is a laboratory equipment designed for rapid and efficient disruption of biological samples. It utilizes bead-beating technology to homogenize a wide range of sample types, including plant, animal, and microbial tissues. The TissueLyser is a versatile tool that enables efficient sample preparation for various downstream applications.
7
Dc protein assay by Bio-Rad
Sourced in United States, Germany, United Kingdom, Canada, France, Australia, Sweden, Switzerland, Italy, Spain, Belgium, Netherlands, Finland
The DC Protein Assay is a colorimetric assay for the determination of protein concentration. It is based on the reaction of protein with an alkaline copper tartrate solution and Folin reagent, resulting in the reduction of the Folin reagent by the copper-treated proteins. The color change is measured spectrophotometrically, and the protein concentration is determined by comparison to a standard curve.
8
Protein g dynabead by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, Australia, Japan
Protein G Dynabeads are magnetic beads coated with recombinant Protein G. Protein G is a bacterial cell wall protein that binds to the Fc region of immunoglobulins. These beads can be used for the rapid and efficient purification of antibodies from various samples.
9
Histopaque 1077 by Merck Group
Sourced in United States, Germany, United Kingdom, Sao Tome and Principe, Italy, Canada, Macao, Switzerland, Spain, France, Poland, Japan, Ireland, Hungary, Brazil, Norway, Israel, Belgium
Histopaque-1077 is a density gradient medium used for the isolation of mononuclear cells from whole blood. It is a sterile, endotoxin-tested solution composed of polysucrose and sodium diatrizoate, adjusted to a density of 1.077 g/mL.
10
Complete protease inhibitor cocktail by Roche
Sourced in United States, Germany, Switzerland, United Kingdom, China, France, Japan, Canada, Spain, Belgium, Australia, Sweden, Italy, Ireland, Macao
The Complete Protease Inhibitor Cocktail is a laboratory product designed to inhibit a broad spectrum of proteases. It is a concentrated solution containing a mixture of protease inhibitors effective against a variety of protease classes. This product is intended to be used in research applications to preserve the integrity of target proteins by preventing their degradation by proteolytic enzymes.

More about "G 130"

G 130 is a biomolecular compound with diverse research and therapeutic applications.
This versatile molecule exhibits specific biological activities and interactions, making it a subject of keen interest for scientists.
PubCompare.ai, an AI-powered analysis tool, can help researchers locate the most effective G 130 protocols from the vast corpus of literature, preprints, and patents, enhancing the reproducibility of their studies.
By leveraging the power of AI-driven comparisons, PubCompare.ai enables researchers to identify the most effective protocols and products related to G 130, streamlining their research process and improving overall reproducibility.
This can be particularly useful when working with other biomolecules and compounds, such as Benzonase (a recombinant endonuclease), FBS (fetal bovine serum), Protease inhibitor cocktail (a mixture of protease inhibitors), Biorupter (a sonication device), Pierce BCA Protein Assay Kit (a colorimetric assay for protein quantification), TissueLyser (a bead-based homogenization system), DC Protein Assay (a detergent-compatible protein quantification method), Protein G Dynabeads (magnetic beads for protein purification), Histopaque-1077 (a density gradient medium for cell separation), and Complete protease inhibitor cocktail (a comprehensive mixture of protease inhibitors).
Discover the power of PubCompare.ai today and unlock the full potential of G 130 in your research endeavors, enhancing reproducibility and driving your scientific discoveries forward.